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  • Yeast  (38)
  • Springer  (38)
  • American Chemical Society (ACS)
  • National Academy of Sciences
  • 1985-1989  (38)
  • 1980-1984
  • 1987  (38)
Collection
Keywords
Publisher
  • Springer  (38)
  • American Chemical Society (ACS)
  • National Academy of Sciences
  • Wiley-Blackwell  (1)
Years
  • 1985-1989  (38)
  • 1980-1984
Year
  • 1
    Electronic Resource
    Electronic Resource
    Yeast centromere sequences do not confer mitotic stability on circular plasmids containing ARS elements of Tetrahymena thermophila rDNA (1987)
    Springer
    Current genetics 11 (1987), S. 353-357 
    Details
    ISSN: 1432-0983
    Keywords: Tetrahymena ; Replication ; Segregation ; Yeast
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We have previously demonstrated that a 657 bp TaqI-XbaI and a 427 by XbaI-XbaI fragment from the 5′ non-transcribed spacer of the extrachromosomal ribosomal DNA of Tetrahymena thermophila function as autonomously replicating sequences (ARS) in Saccharomyces cerevisiae. These fragments are adjacent to each other in a region that encompasses the in vivo origin of bidirectional replication of rDNA. The presence of a yeast centromere (CEN) fragment does not confer mitotic stability on these plasmids. A sensitive yeast colony colour assay (Hieter et al. 1985a) has been used to evaluate the cis-acting effect of each ARS segment on the pattern of inheritance of a plasmid containing CEN5:URA3:SUP4. Colonies of transformed cells obtained both in the presence and absence of selection were red with no detectable white or pink sectors. The lack of sectoring indicates that both plasmids are lost at an extremely high rate, likely due to 1:0 segregation events. We conclude that while these ARS elements confer a high frequency transformation phenotype, they lack a function which is required in cis for the maintenance of mitotic stability in the presence of a centromere. This missing cis-acting function may result in the inability of the plasmids to be brought under the control of cell-cycle regulated replication.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00378177
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  • 2
    Electronic Resource
    Electronic Resource
    Temperature sensitive pet mutants in yeast Saccharomyces cerevisiae that lose mitochondrial RNA (1987)
    Springer
    Current genetics 11 (1987), S. 359-367 
    Details
    ISSN: 1432-0983
    Keywords: Yeast ; Mitochondrial ; Mutants ; RNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary This is a description of a new class of temperature sensitive pet mutants in Saccharomyces cereviase that lose all or part of their mitochondrial RNA at the restrictive temperature. These mutants fall into 8 different complementation groups, mna1 to mna8, and 2 different classes based on their phenotype. Class I mutations, mna1-1 through mna5-1, cause complete or partial loss of mitochondrial RNA at the restrictive temperature. The mutation, mna1-1, is especially interesting since it causes a loss of both mitochondrial DNA and RNA when the mutant is grown on a fermentable carbon source at the restrictive temperature. However, when this mutant is grown at the permissive temperature on a non-fermentable carbon source then shifted to the restrictive temperature, only the mitochondrial RNA is lost. This indicates that the primary cause for the pet phenotype is due to the loss of mitochondrial RNA and not DNA. Class II mutations, mna6-1 through man8-1, cause complete loss of the 14S rRNA after growth at the restrictive temperature in a fermentable carbon source. This loss appears to be specific for the 14S rRNA, since all other transcripts probed by Northern analysis are normal.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00378178
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  • 3
    Electronic Resource
    Electronic Resource
    The disomy for chromosome IV and the spontaneous rho- mutability in Saccharomyces cerevisiae (1987)
    Springer
    Current genetics 11 (1987), S. 407-410 
    Details
    ISSN: 1432-0983
    Keywords: Yeast ; Disomy for chromosome IV ; Mitochondrial rho − mutability ; Mitotic chromosome loss
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The disomy for chromosome IV in the strains studied led to: i) a reduction in the red pigmentation of ade1 mutant colonies; ii) a decrease of the spontaneous rho − mutant frequency, and iii) an impairment of sporulation in hybrids descended from disomic parents. The nuclear srm1 mutation decreasing the spontaneous rho − mutability promoted the spontaneous extra chromosome loss in the disomes for chromosome IV. This result suggests a close connexion between the spontaneous rho − mutability and mitotic chromosome stability.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00378184
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  • 4
    Electronic Resource
    Electronic Resource
    The Yarrowia lipolytica LEU2 gene (1987)
    Springer
    Current genetics 11 (1987), S. 377-383 
    Details
    ISSN: 1432-0983
    Keywords: Y. lipolytica ; LEU2 ; Yeast ; Leucine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A 2810 by DNA fragment containing the beta-isopropylmalate dehydrogenase gene of the dimorphic yeast Yarrowia lipolytica has been sequenced. The sequence contains an open reading frame of 405 codons, predicting a protein of 43,366 molecular weight. Protein sequence homology with the polypeptide encoded by the LEU2 gene of Saccharomyces cerevisiae is 64%, whereas DNA sequence homology is 61%. The 5′- and 3′-flanking regions of the Y. lipolytica LEU2 gene share only some general structural features common to genes of S. cereviside such as the presence and location of TATA boxes, CAAT boxes, CACACA repeats, the lack of G residues in the 5′-untranslated region and 3′-transcription terminators. Transcription of a 1.4 kb mRNA begins at a small cluster of sites approximately 40 base pairs before the initial ATG.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00378180
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  • 5
    Electronic Resource
    Electronic Resource
    Genetic modification of the spontaneous rho − mutability in Saccharomyces cerevisiaee (1987)
    Springer
    Current genetics 11 (1987), S. 411-413 
    Details
    ISSN: 1432-0983
    Keywords: Yeast ; Mitochondrial rho − mutability ; Genetic analysis ; Modifying genes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The phenotypic trait “starry colony” in Saccharomyces is associated with a high spontaneous rho − petite mutability. Genetic analysis of this trait has shown the high rho − mutability to be caused by several modifying genes present together in the strains studied. Every single modifying gene produces only a relatively small enhancement of the rho − mutability.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00378185
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  • 6
    Electronic Resource
    Electronic Resource
    Base analogue mutagenesis in yeast and its modulation by pyrimidine deoxynucleotide pool imbalances: incorporation of bromodeoxyuridylate and iododeoxyuridylate (1987)
    Springer
    Current genetics 11 (1987), S. 421-427 
    Details
    ISSN: 1432-0983
    Keywords: Yeast ; Mutagenesis ; Base analogues
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Cells of the yeast, Saccharomyces cerevisiae, which are auxotrophic for thymidylate (tmp1) can also incorporate analogues of thymidylate. When the base analogue, 5-bromodeoxyuridylate, is incorporated into tmp1 yeast cells it is lethal and mutagenic. Both lethality and mutation induction can be drastically altered by perturbation of the pyrimidine nucleotide pools. Analysis of mutation induction, bromodeoxyuridylate incorporation into DNA, and cell viability under various conditions revealed: (1) lethality and mutagenesis can be uncoupled, (2) thymidylate enhances mutagenesis and deoxycytidylate suppresses it, (3) mutation induction is not correlated with the magnitude of bromodeoxyuridylate incorporation into DNA. Therefore, in yeast, the pyrimidine nucleotide pools have a powerful effect on bromodeoxyuridylate mutagenesis. Both bromodeoxyuridylate and iododeoxyuridylate are extensively incorporated into the DNA of tmp1 yeast cells; however, iododeoxyuridylate is non-mutagenic. Replication proceeds at the same rate in the presence of the natural substrate or either analogue. When cells are supplied with thymidylate and bromodeoxyuridylate together, there is no discrimination against bromodeoxyuridylate as a DNA precursor. However, in the presence of thymidylate and iododeoxyuridylate, there is a 3 to 1 discrimination against iododeoxyuridylate as compared to thymidylate.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00384602
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  • 7
    Electronic Resource
    Electronic Resource
    Extrachromosomal genetics in the yeast Kluyveromyces lactis. Isolation and characterization of antimycin-resistant mutants (1987)
    Springer
    Current genetics 11 (1987), S. 475-482 
    Details
    ISSN: 1432-0983
    Keywords: Kluyveromyces lactis ; Yeast ; Extrachromosomal inheritance ; Antimycin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Antimycin-resistant (AR) mutants of the yeast Kluyveromyces lactis, obtained either spontaneously or after manganese treatment, were isolated and genetically characterized. Most of the mutants obtained after manganese mutagenesis and two spontaneous mutants, tolerated high antimycin concentrations (more than 10 /gmg/ml) and were extrachromosomal. One mutant which grew only in low antimycin (1 /gmg/ml) showed a Mendelian type of inheritance. The extrachromosomal mutants could be assigned to at least two genetic loci (A I R and A II R ). Mutants representative of these two groups showed increased resistance to the antibiotic when the respiration of whole cells or mitochondria was studied. Extrachromosomal mutants of Saccharomyces cerevisiae resistant to antimycin were also induced with manganese, isolated and characterized. Comparative studies of the antimycin-resistant mutants of K. lactis and S. cerevisiae permitted the following observations: a) K. lactis is more resistant to antimycin, funiculosin, mucidin and diuron than S. cerevisiae, as are the AR mutants; b) K. lactis shows correlated sensitivity to funiculosin differing in this aspect from S. cerevisiae; c) the antimycin-resistant mutants of K. lactis belonging to group 11 (A II R ) were also resistant to diuron, tolerating concentrations of more than 200 /gmg/ml; d) all extrachromosomal antimycin-resistant-mutants of S. cerevisiae and some of the AR mutants of K. lactis were more sensitive to mucidin than the wild type.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00384609
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  • 8
    Electronic Resource
    Electronic Resource
    Phenotypic suppression and nuclear accommodation of the mit − oxi1-V25 mutation in isolated yeast mitochondria (1987)
    Springer
    Current genetics 12 (1987), S. 305-310 
    Details
    ISSN: 1432-0983
    Keywords: Yeast ; Mitochondria ; Translation ; Informational Suppression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Phenotypic suppression by the antibiotic, paromomycin, of the mitochondrial oxi1 −-V25 mutation, a mutation which arrests by premature ochre codon the synthesis of the cox 11 subunit, was studied in isolated yeast mitochondria competent in translation. This antibiotic is known to suppress the mutation in vivo (Dujardin et al. 1984) and allowed in vitro, at concentrations of 20–1100 Mg per ml. the synthesis of the cox II subunit. This strongly suggests that phenotypic suppression of mit − mutations is due to the direct action of paromomycin on mitochondrial ribosomes. The effect of paromomycin bears a resemblance to the function of the omnipotent nuclear suppressor mutation R705. The nuclear suppression was expressed in isolated mitochondria; suppressor mutation influenced the structure of the mitoribosome. Therefore, it appears that mitoribosomes are indeed the common target in the phenotypical and genetic nuclear suppression of the oxi1-V25 mutation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00405752
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  • 9
    Electronic Resource
    Electronic Resource
    ARS activity along the yeast mitochondrial apocytochrome b region: correlation with the location of petite genomes and consensus sequences (1987)
    Springer
    Current genetics 12 (1987), S. 583-589 
    Details
    ISSN: 1432-0983
    Keywords: Yeast ; ARS-like activity ; Petite genome replication
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Seven MboI fragments spanning the mitochondrial apocytochrome b gene in Saccharomyces cerevisiae strain D273-10B were cloned in the BamHI site of the integrative yeast vector YIp5 and the capacity for autonomous replication was subsequently assayed in yeast. The positive correlation found between the ars-like activity in four fragments and the presence of regions common to multiple ethidium bromide-induced petite (rho−) genomes suggests that the mitochondrial sequences possibly active as origins of replication in low-complexity neutral or weakly suppressive rho− mutants could be functionally related to the yeast nuclear replicator 11 nucleotide motif defined by Broach et al. (1983).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00368060
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  • 10
    Electronic Resource
    Electronic Resource
    A mitochondrial frameshift-suppressor (+) of the yeast S. cerevisiae maps in the mitochondrial 15S rRNA locus (1987)
    Springer
    Current genetics 11 (1987), S. 295-301 
    Details
    ISSN: 1432-0983
    Keywords: Yeast ; Mitochondrial ; Frameshift-Suppression ; 15S rRNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The first case of a +1 “extrageneic” frameshift suppressor (MF1), mapping in the yeast mitochondrial 15S rRNA gene is reported. The suppressor was identified by genetic analyses in a leaky mitochondrial oxi1 frameshift mutant and the respective wild-type strain 777-3A of the yeast S. cerevisiae. This is in accordance with the finding that all mitochondrial frameshift mutants isolated from this strain tend to be leaky to a variable degree. MF1 does not suppress known nonsense mutations created by a direct basepair exchange in strain 777-3A. These mutants exhibit a non-leaky phenotype (Weiss-Brummer et al. 1984).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00355403
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