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  • Transfection
  • American Association for the Advancement of Science (AAAS)  (8)
  • American Association of Petroleum Geologists (AAPG)
  • Wiley
  • 1990-1994
  • 1985-1989  (8)
  • 1955-1959
  • 1986  (8)
Collection
Keywords
Publisher
  • American Association for the Advancement of Science (AAAS)  (8)
  • American Association of Petroleum Geologists (AAPG)
  • Wiley
Years
  • 1990-1994
  • 1985-1989  (8)
  • 1955-1959
Year
  • 1
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    Structural heterogeneity and functional domains of murine immunoglobulin G Fc receptors (1986)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1986-11-07
    Description: Binding of antibodies to effector cells by way of receptors to their constant regions (Fc receptors) is central to the pathway that leads to clearance of antigens by the immune system. The structure and function of this important class of receptors on immune cells is addressed through the molecular characterization of Fc receptors (FcR) specific for the murine immunoglobulin G isotype. Structural diversity is encoded by two genes that by alternative splicing result in expression of molecules with highly conserved extracellular domains and different transmembrane and intracytoplasmic domains. The proteins encoded by these genes are members of the immunoglobulin supergene family, most homologous to the major histocompatibility complex molecule E beta. Functional reconstitution of ligand binding by transfection of individual FcR genes demonstrates that the requirements for ligand binding are encoded in a single gene. These studies demonstrate the molecular basis for the functional heterogeneity of FcR's, accounting for the possible transduction of different signals in response to a single ligand.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Ravetch, J V -- Luster, A D -- Weinshank, R -- Kochan, J -- Pavlovec, A -- Portnoy, D A -- Hulmes, J -- Pan, Y C -- Unkeless, J C -- AI 24322/AI/NIAID NIH HHS/ -- GM 36306/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 1986 Nov 7;234(4777):718-25.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/2946078" target="_blank"〉PubMed〈/a〉
    Keywords: Amino Acid Sequence ; Animals ; Base Sequence ; DNA/genetics ; Gene Expression Regulation ; Histocompatibility Antigens Class II/genetics ; Immunoglobulin G ; Lymphocytes/*physiology ; Macrophages/*physiology ; Membrane Proteins ; Mice ; Protein Conformation ; *Receptors, Fc/genetics ; Receptors, IgG ; Transcription, Genetic ; Transfection
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
    Published by American Association for the Advancement of Science (AAAS)
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  • 2
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    Expression and characterization of the trans-activator of HTLV-III/LAV virus (1986)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1986-11-21
    Description: The human T-lymphotropic retrovirus HTLV-III/LAV encodes a trans-activator that increases viral gene expression. We expressed this trans-activator in animal cells and studied its structural and functional characteristics. The putative trans-activator protein was immunoprecipitated from overproducing stable cell lines and shown to migrate as a 14-kilodalton polypeptide on sodium dodecyl sulfate-polyacrylamide gels. S1 nuclease mapping experiments showed that the trans-activator increases the levels of steady-state messenger RNA transcribed from the viral long terminal repeat promoter. Sequences within the R region of the HTLV-III/LAV long terminal repeat are essential for trans-activation. Quantitations of messenger RNA and protein showed that the protein increase was greater than the messenger RNA increase in CV1 and HeLa cells, indicating that more than one mechanism was responsible for the trans-activation and that cell type-specific factors may determine the final level of trans-activation.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Wright, C M -- Felber, B K -- Paskalis, H -- Pavlakis, G N -- N01-CO-23909/CO/NCI NIH HHS/ -- New York, N.Y. -- Science. 1986 Nov 21;234(4779):988-92.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/3490693" target="_blank"〉PubMed〈/a〉
    Keywords: Cell Line ; Electrophoresis, Polyacrylamide Gel ; Gene Products, rev ; HIV/*genetics ; Molecular Sequence Data ; Nucleic Acid Hybridization ; RNA, Messenger/analysis ; Retroviridae Proteins/*metabolism ; Transfection ; Viral Proteins/*biosynthesis ; Virus Activation ; rev Gene Products, Human Immunodeficiency Virus
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
    Published by American Association for the Advancement of Science (AAAS)
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  • 3
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    Tissue-specific expression in transgenic mice of a fused gene containing RSV terminal sequences (1986)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1986-03-28
    Description: Transgenic mice were generated with pRSV-CAT, a chimeric gene construct containing the long terminal repeat of Rous sarcoma virus (RSV) linked to the bacterial gene encoding chloramphenicol acetyltransferase (CAT). CAT expression, detected in adult animals of five independent strains, was preferentially directed to organs rich in tendon, bone, and muscle. This pattern reflects the disease specificity of the intact virus and suggests that the tissue tropism of RSV is determined at least in part by the presence of endogenous tissue-specific factors that can promote expression of genetic information linked to the long terminal repeat. In two of the mouse strains, insertion of the pRSV-CAT DNA resulted in developmental abnormalities. One of these strains was characterized by a dominant trait of embryonic lethality, the other by a recessive trait of fused toes in all four feet.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Overbeek, P A -- Lai, S P -- Van Quill, K R -- Westphal, H -- New York, N.Y. -- Science. 1986 Mar 28;231(4745):1574-7.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/3006249" target="_blank"〉PubMed〈/a〉
    Keywords: Acetyltransferases/genetics ; Age Factors ; Animals ; Avian Sarcoma Viruses/*genetics ; Chloramphenicol O-Acetyltransferase ; DNA, Viral/*genetics ; Gene Expression Regulation ; Genes, Regulator ; Limb Deformities, Congenital ; Mice ; Repetitive Sequences, Nucleic Acid ; Tissue Distribution ; Transcription, Genetic ; Transfection
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
    Published by American Association for the Advancement of Science (AAAS)
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  • 4
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    In vivo competition between a metallothionein regulatory element and the SV40 enhancer (1986)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1986-04-04
    Description: The human metallothionein-IIA (hMT-IIA) gene contains an enhancer element within its 5' regulatory region. This enhancer element can compete with the SV40 enhancer for one or more cellular factors in vivo. The competition between the two elements is modulated by cadmium, an inducer of hMT-IIA transcription. The data presented are consistent with a model in which heavy metal ions control the ability of the hMT-IIA enhancer to bind a positive factor, leading to increased transcription. The same factor is required for maximal activity of the SV40 enhancer, which suggests that viruses utilize factors that have a normal role in cellular gene expression to control their own genes.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Scholer, H -- Haslinger, A -- Heguy, A -- Holtgreve, H -- Karin, M -- ES03222/ES/NIEHS NIH HHS/ -- New York, N.Y. -- Science. 1986 Apr 4;232(4746):76-80.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/3006253" target="_blank"〉PubMed〈/a〉
    Keywords: Acetyltransferases/genetics ; Animals ; Base Sequence ; Cadmium/pharmacology ; Cell Line ; Cercopithecus aethiops ; Chloramphenicol O-Acetyltransferase ; *Enhancer Elements, Genetic ; *Genes ; *Genes, Regulator ; *Genes, Viral ; Humans ; Kidney ; Kinetics ; Metallothionein/*genetics ; Plasmids ; Simian virus 40/*genetics ; Transcription, Genetic/drug effects ; Transfection
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
    Published by American Association for the Advancement of Science (AAAS)
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  • 5
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    Primary rat embryo cells transformed by one or two oncogenes show different metastatic potentials (1986)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1986-04-11
    Description: Second-passage rat embryo cells were transfected with a neomycin resistance gene and the activated form of the c-Ha-ras I gene, or with these two genes plus the adenovirus type 2 E1a gene. Foci of morphologically transformed cells were observed in both cases; however, the frequency of transformation was at least ten times higher with two oncogenes than with the ras gene alone. All the transformed cell lines gave rise to rapidly growing tumors when injected subcutaneously into nude mice. All but one of the cell lines transformed by the ras oncogene alone formed metastatic nodules in the lungs of animals that had been injected subcutaneously with transformed cells. When transformed cells were injected intravenously, all the ras single-gene transformants gave rise to many metastatic lung nodules. In contrast, cell lines transformed with ras and E1a did not generate metastases after subcutaneous injection and gave rise to very few metastatic lung nodules after intravenous injection. These data demonstrate that a fully malignant cell with metastatic potential, as measured in an immunodeficient animal, can be obtained from early passage embryo cells by the transfection of the ras oncogene alone.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Pozzatti, R -- Muschel, R -- Williams, J -- Padmanabhan, R -- Howard, B -- Liotta, L -- Khoury, G -- 3F32CA07245-02/CA/NCI NIH HHS/ -- New York, N.Y. -- Science. 1986 Apr 11;232(4747):223-7.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/3456644" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Carcinoma/genetics ; Cell Line ; Cell Transformation, Neoplastic/*metabolism ; Cricetinae ; Genetic Engineering ; Mice ; Mice, Nude ; *Oncogenes ; Plasmids ; Rats/embryology ; Rats, Inbred Strains/embryology ; Transfection ; Urinary Bladder Neoplasms/genetics
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
    Published by American Association for the Advancement of Science (AAAS)
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  • 6
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    Important advance in gene therapy (1986)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1986-07-11
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Lewin, R -- New York, N.Y. -- Science. 1986 Jul 11;233(4760):160.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/3460175" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; *Genetic Engineering ; Humans ; Mice ; Transfection
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 7
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    The application of bone marrow transplantation to the treatment of genetic diseases (1986)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1986-06-13
    Description: Genetic diseases can be treated by transplantation of either normal allogeneic bone marrow or, potentially, autologous bone marrow into which the normal gene has been inserted in vitro (gene therapy). Histocompatible allogeneic bone marrow transplantation is used for the treatment of genetic diseases whose clinical expression is restricted to lymphoid or hematopoietic cells. The therapeutic role of bone marrow transplantation in the treatment of generalized genetic diseases, especially those affecting the central nervous system, is under investigation. The response of a generalized genetic disease to allogeneic bone marrow transplantation may be predicted by experiments in vitro. Gene therapy can be used only when the gene responsible for the disease has been characterized. Success of gene therapy for a specific genetic disease may be predicted by its clinical response to allogeneic bone marrow transplantation.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Parkman, R -- New York, N.Y. -- Science. 1986 Jun 13;232(4756):1373-8.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/3520819" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; *Bone Marrow Transplantation ; Enzymes/deficiency/genetics ; Genetic Diseases, Inborn/*therapy ; Genetic Engineering ; Humans ; Mice ; Transfection
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 8
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    AIDS retrovirus (ARV-2) clone replicates in transfected human and animal fibroblasts (1986)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1986-05-23
    Description: A molecular clone of the AIDS-associated retrovirus (ARV-2) was transfected into human T lymphocyte and monocyte cell lines as well as mouse, mink, monkey, and human fibroblast lines. A replicating virus with cytopathic and biologic properties of ARV-2 was recovered from all the cell lines. The animal and human fibroblast cells are resistant to direct infection by ARV, and in these experiments virus production in the fibroblast lines, especially mouse, was reduced compared to human lymphocytes. However, human fibroblasts were more permissive to virus expression than mouse cells. These results show that, whereas the primary block to ARV infection in certain cells may occur at the cell surface, intracellular mechanisms can also participate in controlling virus replication. The results have relevance to vaccine development and encourage further work with modified molecular clones to examine regions of the ARV genome necessary for cytopathology and replication.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Levy, J A -- Cheng-Mayer, C -- Dina, D -- Luciw, P A -- CA34980/CA/NCI NIH HHS/ -- New York, N.Y. -- Science. 1986 May 23;232(4753):998-1001.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/3010461" target="_blank"〉PubMed〈/a〉
    Keywords: Acquired Immunodeficiency Syndrome/*microbiology ; Animals ; Cloning, Molecular ; Cytopathogenic Effect, Viral ; Deltaretrovirus/*growth & development ; Fibroblasts/microbiology ; Humans ; Species Specificity ; Transfection ; Virus Replication
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
    Published by American Association for the Advancement of Science (AAAS)
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