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  • Immunohistochemistry
  • Springer  (24)
  • American Chemical Society
  • Essen : Verl. Glückauf
  • 2015-2019
  • 2005-2009
  • 1985-1989  (24)
  • 1986  (24)
Collection
Keywords
Publisher
  • Springer  (24)
  • American Chemical Society
  • Essen : Verl. Glückauf
Years
  • 2015-2019
  • 2005-2009
  • 1985-1989  (24)
Year
  • 1
    Electronic Resource
    Electronic Resource
    Immunohistochemical detection of subunit A(M) of lactate dehydrogenase in tissues of the developing chick (1986)
    Springer
    Development genes and evolution 195 (1986), S. 409-416 
    Details
    ISSN: 1432-041X
    Keywords: Immunohistochemistry ; Tissue distribution of LDH5 ; Developing chicken embryos
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The distribution of A(M) subunits of lactate dehydrogenase (mainly LDH5) in developing muscle, heart, liver, lung, kidney and cartilage tissue of chicken embryos was examined by the indirect fluorescent antibody technique. Antibodies against porcine LDH5, purified by affinity chromatography, were used for this purpose. In special areas of newly formed myofibrils in somitic myoblasts fluorescence was already detected after 4 days of incubation, and located at the same place in muscle tissue of all advanced developmental stages examined. During the myotube stage of muscle development staining was also located in the peripheral thickened cytoplasma of the myotubes. The myocardium did not exhibit any fluorescent staining in the developmental stages examined. Endocardium, epicardium and pericardium, however, were fluorescent in young developmental stages. The liver showed fluorescence in 5- to 8-day embryos mainly in the endothelial cells of the blood sinusoids. In 9- to 12-day embryos the bile ducts became fluorescent. In lungs after 9- to 12-day development the epithelium and the surrounding tissues of bronchi exhibited strong immunofluorescence. The mesonephros exhibited faint granular fluorescence in tubule-forming cells and their membranes after 4–9 days of incubation. Advanced developmental stages only exhibited fluorescent blood cells. This latter staining is at least partly due to non-specific reactions of blood cell membranes with FITC-conjugated anti-rabbit IgG. Cartilage is characterized by non-specific fluorescence, but in embryos older than 8 days strong granular fluorescence of chondrocytes and staining of the perichondrium distinguished sections treated with anti-LDH5 antibodies from control sections reacted only with FITC-conjugated anti-rabbit IgG. In addition, strong fluorescent staining was detectable in certain areas of the 5-day neural tube and faint staining in the mucosa of the intestine from embryos older than 10 days.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00375745
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  • 2
    Electronic Resource
    Electronic Resource
    Immunohistochemical evidence for a magnocellular somatostatin cell group in the anterior paraventricular thalamus of the rat (1986)
    Springer
    Cell & tissue research 246 (1986), S. 683-685 
    Details
    ISSN: 1432-0878
    Keywords: Somatostatin (SRIF, GHR-IF) ; Neuropeptides ; Immunohistochemistry ; Thalamus ; Retrograde tracing ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary By use of the indirect immunofluorescence technique a small group of large somatostatin-positive neurons is described in the subependymal area of the anterior paraventricular thalamus of the male rat. Retrograde-tracing experiments suggest that they project to areas outside the blood-brain barrier.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00215212
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  • 3
    Electronic Resource
    Electronic Resource
    Immunocytochemical and biochemical localization of parvalbumin in the retina (1986)
    Springer
    Cell & tissue research 243 (1986), S. 213-217 
    Details
    ISSN: 1432-0878
    Keywords: Ca2+-binding protein ; Parvalbumin ; Retina ; Immunohistochemistry ; Radioimmunoassay ; Mammals
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The cellular distribution of parvalbumin-like immunoreactivity (PA-LI) in normal retina of rat, monkey, and human was investigated by immunohistochemical peroxidase antiperoxidase methods, and the levels of PA-LI in normal rat retina and brain were measured by radioimmunoassay. The antibody, raised in rabbits using rat skeletal muscle parvalbumin, did not cross-react with other Ca2+-binding proteins such as calmodulin or S-100 proteins. In rat retina, PA-LI-containing cells are located in the proximal inner nuclear layer and send processes to the external half of the internal plexiform layer, suggesting that they are amacrine cells. In monkey and human retina, PA-LI positive cells exist in the outermost sublayer of inner nuclear layer, and PA-LI-containing fibers that extend horizontally are found in the internal zone of outer plexiform layer. The radioimmunoassay revealed that the rat retina contained 1710±91 ng PA-LI/mg protein, the levels of which were higher than that of brain (881±165 ng PA-LI/mg protein). These results show an additional location for PA-LI outside skeletal muscle and brain, and also provide information on the function of interneurons of retina, which are still poorly understood.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00221870
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  • 4
    Electronic Resource
    Electronic Resource
    Distributional pattern of serotonin-immunoreactive nerve fibers in the lateral geniculate nucleus of the rat, cat and monkey (Macaca fuscata) (1986)
    Springer
    Cell & tissue research 243 (1986), S. 249-253 
    Details
    ISSN: 1432-0878
    Keywords: Serotonin-immunoreactive nerve fibers ; Lateral geniculate nucleus ; Immunohistochemistry ; Rat ; Cat ; Monkey (Macaca fuscata)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The distribution of serotonin-containing nerve fibers in the lateral geniculate nucleus (LGN) of the rat, cat, and monkey (Macaca fuscata) was studied by use of the peroxidase-antiperoxidase method and an antiserum against serotonin. In all three species, the pattern of fibers was denser in the ventral portion of the LGN (LGNv) than in the dorsal nuclear portion (LGNd). In the LGNd of rat, serotonin-immunoreactive fibers were evenly distributed in the form of a dense network, but in cat and monkey there were marked regional differences. Serotonin-immunoreactive elements were most numerous in the C complex and medial interlaminal nucleus of cat, and in the S layer and interlaminar zones of Macaca fuscata.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00251038
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  • 5
    Electronic Resource
    Electronic Resource
    Distribution and characterization of the opioid octapeptide met5-enkephalin-arg6-gly7-leu8 in the gastrointestinal tract of the rat (1986)
    Springer
    Cell & tissue research 244 (1986), S. 77-85 
    Details
    ISSN: 1432-0878
    Keywords: Met5-enkephalin-arg6-gly7-leu8 ; Enkephalin ; Gastrointestinal tract ; Immunohistochemistry ; Opioid peptides ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The distribution and characterization of the opioid octapeptide met5-enkephalin-arg6-gly7-leu8 (met5-enk-arg6-gly7-leu8) within the gastrointestinal tract of the rat has been determined by immunohistochemistry and radioimmunoassay by use of a newly developed antibody to met5-enk-arg6-gly7-leu8. With both techniques, met5-enk-arg6-gly7-leu8-immunoreactivity (met5-enk-arg6-gly7-leu8IR) was detected in all regions of the gastrointestinal (GI) tract except the esophagus. The highest concentration of immunoreactive met5-enk-arg6-gly7-leu8 was observed in the colon, while intermediate concentrations were found in the stomach, duodenum, jejunum, and ileum. Immunostained somata were observed chiefly in the myenteric plexus; immunostained processes were present primarily in the myenteric plexus and the circular muscle layer. This distribution pattern is similar to that previously observed with antiserum to met5-enkephalin-arg6-phe7 (met5-enk-arg6phe7). Chromatographic analysis of met5-enk-arg6-gly7leu8-immunoreactive peptides extracted from the GI tract revealed the presence of an immunoreactive peptide of high molecular weight which accounted for approximately three-quarters of met5-enk-arg6-gly7-leu8-IR in both stomach and colon. These findings suggest a role for peptides related to the octapeptide met5-enk-arg6-gly7-leu8 in the regulation of GI function.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00218384
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  • 6
    Electronic Resource
    Electronic Resource
    Differences in marker expression among branched histiocytic cells in T-cell areas of the lymphoreticular system and among their epidermis- and mucosa-associated equivalents (1986)
    Springer
    Cell & tissue research 244 (1986), S. 471-478 
    Details
    ISSN: 1432-0878
    Keywords: Branched histiocytic cells ; T-zone histiocytes ; Intraepithelial histiocytes ; Immunohistochemistry ; Man
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Branched histiocytic cells of the epidermis, the oral and anal mucosa, the tonsillar crypt epithelium, the thymus and of the T-cell-dependent areas of lymph node, spleen, and tonsil were examined with immunohistochemical single- and double-staining techniques. The markers used were a monoclonal anti-T6-antibody, a monoclonal anti-HLA-DR-antibody, heteroantiserum to S-100 protein and peanut agglutinin. Anti-HLA-DR and peanut agglutinin reacted with a considerable number of branched histiocytic cells, whereas anti-T6 and anti-S-100 protein only stained relatively small subpopulations. Concerning the population of branched histiocytic cells, double-staining revealed that the tissue distributions of all the markers used overlapped each other to various degrees; this was demonstrated by the different numbers of double-stained cells obtained in the experiments using all six possible combinations of primary reagents. The number of branched histiocytic cells co-expressing the markers varied depending upon marker combinations, types of tissue and microenvironment. We suggest that much of the immunologic phenotype of branched histiocytic cells is dynamic rather than static.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00212524
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  • 7
    Electronic Resource
    Electronic Resource
    A reinvestigation of the Gn-RH (gonadotrophin-releasing hormone) systems in the goldfish brain using antibodies to salmon Gn-RH (1986)
    Springer
    Cell & tissue research 244 (1986), S. 327-337 
    Details
    ISSN: 1432-0878
    Keywords: Gonadotrophin-releasing hormone ; Teleosts ; Immunohistochemistry ; Neuroendocrine control ; Reproduction ; Goldfish (Carassius auratus)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The organization of Gn-RH systems in the brain of teleosts has been investigated previously by immunohistochemistry using antibodies against the mammalian decapeptide which differs from the teleostean factor. Here, we report the distribution of immunoreactive Gn-RH in the brain of goldfish using antibodies against synthetic teleost peptide. Immunoreactive structures are found along a column extending from the rostral olfactory bulbs to the pituitary stalk. Cell bodies are observed within the olfactory nerves and bulbs, along the ventromedial telencephalon, the ventrolateral preoptic area and the latero-basal hypothalamus. Large perikarya are detected in the dorsal midbrain tegmentum, immediately caudal to the posterior commissure. A prominent pathway was traced from the cells located in the olfactory nerves through the medial olfactory tract and along all the perikarya described above to the pituitary stalk. In the pituitary, projections are restricted to the proximal pars distalis. A second immunoreactive pathway ascends more dorsally in the telencephalon and arches to the periventricular regions of the diencephalon. Part of this pathway forms a periventricular network in the dorsal and posterior hypothalamus, whereas other projections continue caudally to the medulla oblongata and the spinal cord. Lesions of the ventral preoptic area demonstrate that most of the fibers detected in the pituitary originate from the preoptic region.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00219208
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  • 8
    Electronic Resource
    Electronic Resource
    Evidence for the presence of Calbindin-D 28K (CaBP-28K) in the tibial growth cartilages of rats (1986)
    Springer
    Cell & tissue research 245 (1986), S. 331-335 
    Details
    ISSN: 1432-0878
    Keywords: Calbindin-D 28K (CaBP-28K) ; Immunohistochemistry ; Growth cartilage ; Vitamin D ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The distribution of the vitamin-D dependent calcium-binding protein (Calbindin-D 28K) (CaBP-28K) in the tibial growth plate cartilage of the rat has been studied immunohistochemically using an antibody raised against rat renal CaBP-28K. The protein was detected mainly in the nuclei of chondrocytes and occasionally in the juxta-nuclear cytoplasm. The distribution was not uniform throughout the growth plate, but concentrated in the proliferatively active chondrocytes of the resting and proliferative zones. These findings raise the possibility that CaBP-28K may be involved in the mitotic activity of the chondrocytes, acting as a regulator of the proliferative process, perhaps via intranuclear calcium.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00213939
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  • 9
    Electronic Resource
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    Immunohistochemical localization of urotensin I and other neuropeptides in the caudal neurosecretory system of three species of teleosts and two species of elasmobranchs (1986)
    Springer
    Cell & tissue research 244 (1986), S. 687-690 
    Details
    ISSN: 1432-0878
    Keywords: Caudal neurosecretory system ; Urotensin ; Neuropeptides ; Teleosts ; Elasmobranchs ; Immunohistochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In three species of teleosts — carp Cyprinus carpio; grass carp Ctenopharyngodon idella; and crucian carp Carassius auratus — the caudal neurosecretory system displays small, medium-sized and large neurons. Urotensin I (UI)-immunoreactive and UI-nonreactive neurons were found in all three groups; in general, the number of the latter neurons exceeded that of the former. Noteworthy are: (i) UI-immunoreactive fibers in the caudal spinal cord and (ii) dense accumulations of UI-immunoreactive product around the capillaries of the urophysis. In two species of elasmobranchs — cat shark Heterodontus japonicas and swell shark Cephaloscyllium umbratile — neurosecretory neurons decreased in size in rostro-caudal direction. Most of the neurosecretory perikarya, their axons and the corresponding neurohemal areas were UI-immunoreactive, but a small number of secretory neurons was devoid of immunoreaction. Oxytocin, arginine vasopressin, substance P, somatostatin, neurotensin, vasoactive intestinal polypeptide and gastrin-releasing peptide were not detected in the caudal neurosecretory system of the carp.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00212550
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  • 10
    Electronic Resource
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    Light-microscopic immunocytochemical localization of growth hormone-releasing factor in the human hypothalamus (1986)
    Springer
    Cell & tissue research 245 (1986), S. 461-463 
    Details
    ISSN: 1432-0878
    Keywords: Growth hormone ; Releasing factor ; Immunohistochemistry ; Hypothalamus ; Pituitary stalk ; Man
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The distribution of growth hormone-releasing factor (GRF)-like immunoreactivity in the human hypothalamus was studied by light-microscopic immunocytochemistry. With antibodies that we developed against synthetic human pancreatic GRF (hpGRF), we localized GRF immunoreactivity in neuronal cell bodies that were observed only in the infundibular (arcuate) nucleus. Immunostained nerve fibers were found in large numbers in the neurovascular zone of the median eminence, in the proximal portion of the pituitary stalk and in periventricular areas. These localizations are in agreement with those of studies recently performed in other species and strongly suggest that GRF can be released into the capillaries of the pituitary portal plexus to reach the anterior pituitary gland. The projections of GRF neurons in extra-infundibular regions suggest that GRF can also act as a neuromodulator or neurotransmitter in the hypothalamus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00213956
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  • 11
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    Characteristic pattern of monoaminergic nerve fibers in the pineal organ of the monkey, Macaca fuscata (1986)
    Springer
    Cell & tissue research 245 (1986), S. 453-456 
    Details
    ISSN: 1432-0878
    Keywords: Pineal organ ; Noradrenergic fibers ; Serotonin ; Fluorescence histochemistry ; Immunohistochemistry ; Monkey, Macaca fuscata
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Monoaminergic nerve fibers were studied in the pineal organ of the monkey, Macaca fuscata, by use of fluorescence and immunohistochemical procedures. Abundant formations of noradrenergic nerve fibers were observed in the pineal organ. They entered the parenchyma in the form of several coarse bundles via the capsule in the distal portion of the organ and spread throughout the organ after branching into smaller units. The density of the autonomic innervation decreased gradually toward the proximal portion of the organ. In the distal portion, numerous nerve fibers formed perivascular plexuses around the blood vessels and some fibers ran as bundles unrelated to the blood vessels in the stroma. Fine varicose fibers and bundles derived from these plexuses penetrated among the pinealocytes. However, only a few intraparenchymal fluorescent fibers were detected in the proximal third of the gland. With the use of serotonin antiserum serotonin-immunoreactive nerve fibers were clearly restricted to the ventroproximal part of the pineal organ. Although the somata of the pinealocytes showed intense immunoreactivity, their processes were not stained. In one exceptional case, clusters of pinealocytes displaying very intense immunoreactivity were found in an area extending from the distal margin of the ventral portion of the pineal stalk to the proximal portion of the pineal organ proper; these cells were bipolar or multipolar and endowed with well-stained processes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00213954
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  • 12
    Electronic Resource
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    Substance P-like-immunoreactive neurons in the photosensory pineal organ of the rainbow trout, Salmo gairdneri Richardson (Teleostei) (1986)
    Springer
    Cell & tissue research 246 (1986), S. 359-364 
    Details
    ISSN: 1432-0878
    Keywords: Pineal organ ; Neuronal types ; Substance P ; Immunohistochemistry ; Salmo gairdneri (Teleostei)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Restricted numbers of substance P-like-immuno-reactive (SPL-IR) neurons were demonstrated in the photosensory pineal organ of the rainbow trout. The small parapineal organ of this teleost species receives a distinct SPL-IR innervation via the habenular nuclei, but displays no intrinsic SPL-IR neurons. Intrapineal SPL-IR neurons were located in the rostral portion of the pineal end-vesicle. Neuronal somata were found in a lateral position with smooth axonal processes extending mediad. Immunoreactive somata and axonal processes were observed intraparenchymally as well as in the pineal lumen. The pattern of immunoreactivity was not changed in excised pineal organs that had been incubated in tissue culture medium in the dark for 18 h. The possibility that the intrapineal SPL-IR neurons are not part of the neural circuitry involved in the transduction of photic information, but may have other functions, is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00215898
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  • 13
    Electronic Resource
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    The early postnatal development of the primary immune response in TNP-KLH-stimulated popliteal lymph node in the rat (1986)
    Springer
    Cell & tissue research 246 (1986), S. 673-677 
    Details
    ISSN: 1432-0878
    Keywords: Ontogeny ; Lymph node ; Immunohistochemistry ; Primary immune response ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The popliteal lymph nodes were removed from young rats of various ages five days after a single immunization with TNP-KLH in the hind footpads. Cryostat sections of the lymph nodes were investigated by means of enzyme and immunohistochemical techniques at the light-microscopical level. The presence and localization of anti-TNP antibody-containing cells were examined using a new technique to visualize specific antibodies. Moreover, the development of the lymph nodes following exogenous antigenic stimulation was compared with that of unstimulated lymph nodes. Specific antibody-containing cells could not be found before day 15 after birth, in rats immunized at day 10. From that time these lymphoid cells were located primarily at the border between cortex and medulla. Younger popliteal lymph nodes showed only aspecific immunoglobulin-containing lymphoid cells. With age, the number of specific antibody-containing cells tended to increase. These cells were more mature, according to morphological criteria and were located nearer the medulla. The first primary follicles were seen at day 19, as was the case in unstimulated animals. The first secondary follicles, containing germinal centers, were detected at day 23, whereas in unstimulated popliteal lymph nodes they were never found. Trapping of immune complexes could not be demonstrated before day 33 after birth. The later appearance of this phenomenon might be a consequence of the techniques applied to demonstrate specific antibody-containing cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00215210
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  • 14
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    Neuropeptide Y, enkephalin and noradrenaline coexist in sympathetic neurons innervating the bovine spleen (1986)
    Springer
    Cell & tissue research 243 (1986), S. 495-508 
    Details
    ISSN: 1432-0878
    Keywords: Neuropeptide Y ; Enkephalin ; Noradrenaline ; Immunohistochemistry ; Sympathetic neurons ; Neurotransmitters ; Spleen ; Bovine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The subcellular distribution of noradrenaline (NA), neuropeptide Y (NPY), Met and Leu-enkephalin (ENK), substance P (SP), somatostatin (SOM), and vasoactive intestinal polypeptide (VIP) was investigated in homogenates of bovine splenic nerve. The distribution of noradrenergic peptide-containing nerves in the bovine celiac ganglion, splenic nerve and terminal areas in spleen was studied by indirect immunofluorescence histochemistry using antisera to tyrosine hydroxylase (TH), dopamine-β-hydroxylase (DBH), NPY, enkephalin peptides, SP, SOM, VIP and peptide HI (PHI). After density gradient centrifugation, high levels of NPY and ENK-like immunoreactivity (LI) were found in high-density gradient fractions, coinciding with the main NA peak. SP, SOM and VIP were found in fractions with a lower density, VIP being also enriched in a heavy fraction; the latter three peptides were present in low concentrations. Immunohistochemistry revealed that staining for NPYLI and ENK-LI partly overlapped that for TH and DBH in celiac ganglia, splenic nerve axons and terminal areas of spleen. Almost all principal ganglion cells were TH- and DBH-immunoreactive. Many were also NPY-immunoreactive, whereas a smaller number were ENK-positive. In the celiac ganglion patches of dense SP-positive networks and some VIP/PHI- and ENK-immunoreactive fibers were seen around cell bodies. The results indicate that NPY and ENK are stored with NA in large dense-cored vesicles in unmyelinated axons of bovine splenic nerve. SP, SOM and VIP appear in different organelles in axon populations separate from sympathetic noradrenergic nerves.
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    URL: http://dx.doi.org/10.1007/BF00218056
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  • 15
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    Immunohistochemical demonstration of nerves in the predentin and dentin of human third molars with the use of an antiserum against neurofilament protein (NFP) (1986)
    Springer
    Cell & tissue research 243 (1986), S. 469-475 
    Details
    ISSN: 1432-0878
    Keywords: Immunohistochemistry ; Neurofilament protein ; Sensory nerves ; Dentin ; Man
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Immunohistochemistry by use of an antiserum against neurofilament protein (NFP) was applied for staining nerve fibers in the predentin and dentin of human third molars. By devising methods for fixation, decalcification and immunostaining, nerve fibers were clearly and specifically demonstrated in thick (more than 50 μm) sections of teeth. Numerous NFP-positive fibers were distributed in the predentin throughout the coronal region, while a few positive fibers penetrated only a short distance into the dentin. The NFP-positive nerve fibers in the predentin took transverse and complicated courses across, rather than penetrating longitudinally through, the dentinal tubules. Pain sensation in the teeth might be attributable to these complex nerve fibers showing two or three-dimensional extensions.
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    URL: http://dx.doi.org/10.1007/BF00218053
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  • 16
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    Sex differences in pituitary LH storage and release in LHRH-stimulated pubertal rats (1986)
    Springer
    Cell & tissue research 243 (1986), S. 509-515 
    Details
    ISSN: 1432-0878
    Keywords: LHRH ; LH depletion ; Pituitary gland, pars anterior ; Immunohistochemistry ; Radioimmunoassay ; Pubertal rats
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary This study investigates the relationship between pituitary LHRH responsiveness and the depletion of LH in pubertal rats. The anterior pituitaries of 7-week-old rats of both sexes were stimulated for a maximum of 24 h with either a continuous, or pulsatile exposure to LHRH in vitro. Immunohistochemical examination revealed that most LH-cells in females became depleted of immunoreactive material, regardless of the mode of LHRH administration. In contrast, the majority of LH-cells in the male gland retained a strong immunostaining intensity. Radioimmunoassay showed that the initial pituitary LH content was significantly lower in the female rats (P〈 0.001), but, even so, they released a higher percentage of stored LH in response to LHRH stimulation in vitro. A similar result was also obtained after a single injection of LHRH in vivo. Thus, the lower LH content and higher LHRH responsiveness of the female pituitary explain why LHRH treatment induced a pronounced LH depletion in this sex. These results are discussed in relation to available data on heightened LH secretion in maturing female rats.
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    URL: http://dx.doi.org/10.1007/BF00218057
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  • 17
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    Serotonin-like immunoreactivity in the central nervous system and gonad of the scallop, Patinopecten yessoensis (1986)
    Springer
    Cell & tissue research 244 (1986), S. 515-517 
    Details
    ISSN: 1432-0878
    Keywords: Serotonin ; Immunohistochemistry ; Central nervous system ; Gonad ; Patinopecten yessoensis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The localization of neurons containing serotonin in the central nervous system and the gonad of the scallop, Patinopecten yessoensis, was examined immunohistochemically. In the central nervous system a large number of immunoreactive perikarya were observed in the following regions: a part of the anterior lobe of the cerebral ganglion; the posterior lobe of the cerebral ganglion; the pedal ganglion; and the accessory ganglion. No immunoreactive perikarya were found in the visceral ganglion. Numerous immunoreactive fibers were revealed in the neuropil of all central ganglia. In the gonadal region immunoreactive fibers were distributed around the gonoduct and along the germinal epithelium.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00212528
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  • 18
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    Immunohistochemical demonstration of histamine N-methyltransferase-like structures in rat kidney (1986)
    Springer
    Cell & tissue research 243 (1986), S. 681-684 
    Details
    ISSN: 1432-0878
    Keywords: Histamine ; Histamine N-methyltransferase ; Immunohistochemistry ; Kidney ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Histamine N-methyltransferase (S-adenosylmethionine: histamine N-methyltransferase, E.C. 2.1.1.8) was purified to homogeneity from rat kidney, and antibody was raised against it in guinea pigs. The antibody immunoprecipitated histamine N-methyltransferase. Immunofluorescent histochemical studies with anti-histamine N-methyltransferase antibody as the first antibody and goat antiguinea pig IgG conjugated with fluorescein isothiocyanate as the second, showed the presence of immunoreactive structures in the proximal tubules of rat kidney. The brain showed no immunoreaction with the antibody.
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    URL: http://dx.doi.org/10.1007/BF00218079
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  • 19
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    Urotensin I-like immunoreactivity in the midgut endocrine cells of the insects Gryllus bimaculatus and Periplaneta americana (1986)
    Springer
    Cell & tissue research 244 (1986), S. 565-568 
    Details
    ISSN: 1432-0878
    Keywords: Urotensin I ; Gut endocrine cell ; Insects ; Immunohistochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The occurrence of urotensin I (UI)-like immunoreactivity is demonstrated in the midgut of the cricket Gryllus bimaculatus and the cockroach Periplaneta americana. The immunoreactivity is restricted to endocrine cells dispersed in the epithelium of the midgut. The UI-positive endocrine cells are numerous in the cricket, but much fewer in the cockroach. These UI-positive cells are different from bovine pancreatic polypeptide (BPP)-immunoreactive cells which represent a dominant cell type in the midgut of both insects. This study suggests the possibility that the neuropeptide UI or a closely related substance may also occur in animals other than fishes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00212534
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    Electronic Resource
    Electronic Resource
    Ontogenesis of corticotropes and lactotropes in situ in the pituitary gland of the hamster (1986)
    Springer
    Cell & tissue research 245 (1986), S. 673-679 
    Details
    ISSN: 1432-0878
    Keywords: Pituitary gland ; ACTH cells ; PRL cells ; Immunohistochemistry ; Development ; Mesocricetus auratus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The development of corticotropes and lactotropes was investigated in the golden Syrian hamster using an anti-porcine ACTH antiserum and a homologous antihamster PRL antiserum. Oval corticotropes were first visible in the ventral region of the pars distalis at 13 days of gestation. By the end of gestation, corticotropes were found throughout the pars distalis and in the pars intermedia. Corticotropes in the pars distalis of postnatal hamsters were either round or irregularly-shaped, often appearing in clusters. Throughout development, corticotropes often appeared to be surrounding other cells. Scarce, very small lactotropes were first observed in the pars distalis of hamsters on the first postnatal day. The number of these cells, which were either round or polyhedral, increased dramatically between 4 and 20 days of postnatal life. These observations indicate that the sequence of appearance of corticotropes and lactotropes in the hamster is similar to that in other species and that lactotropes are confined to the pars distalis of postnatal hamsters.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00218571
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  • 21
    Electronic Resource
    Electronic Resource
    Ultrastructural and immunohistochemical studies on steroid-secreting cells of the testis and ovary of normal and 3-methylcholanthrene-treated mice (1986)
    Springer
    Cell & tissue research 245 (1986), S. 681-683 
    Details
    ISSN: 1432-0878
    Keywords: Smooth endoplasmic reticulum ; Cytochrome P-450 ; Testis ; Ovary ; Immunohistochemistry ; Mouse
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The testis and ovary of normal and 3-methylcholanthrene-treated mice were studied ultrastructurally and immunohistochemically in order to learn whether steroid-secreting cells of the gonads are involved in drug metabolism. The steroid-secreting cells, i.e., Leydig cells of the testis, and theca interna cells, interstitial gland cells, and corpus luteum cells of the ovary of 3-methylcholanthrene-treated mice, show a strong positive reaction to the antiserum against, hepatic microsomal cytochrome P-450, of liver which is the terminal oxidase of the drug-metabolizing enzyme complex. In addition, it was found that elements of smooth endoplasmic reticulum (SER) in drug-treated mice become well developed as compared with those in control animals. These findings indicate that the steroid secreting cells in testis as well as ovary are involved in the metabolism of both endogenous and exogenous chemical compounds.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00218572
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  • 22
    Electronic Resource
    Electronic Resource
    A comparative in vitro study on LHRH responsiveness of LH cells of the pars tuberalis and pars distalis (1986)
    Springer
    Cell & tissue research 245 (1986), S. 369-375 
    Details
    ISSN: 1432-0878
    Keywords: LHRH stimulation ; Pars distalis ; Pars tuberalis ; Immunohistochemistry ; Radioimmunoassay ; Rat ; Mouse
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The aim of the present study was to test whether the luteinizing-hormone (LH) cells in the pars tuberalis (PT) of the rat and mouse respond to LH-releasing hormone (LHRH) as do those of the pars distalis. A part of the basal hypothalamus containing the pituitary stalk, median eminence and the pars tuberalis (H-PT), was dissected out and incubated in vitro. The LH-secreting capacity of the PT was investigated after removal of the “pituitary body” (i.e., partes distalis, intermedia and nervosa). First, some rat and mouse H-PT tissues were treated with synthetic LHRH (100ng/ml), while others were incubated without LHRH. After 24 h of incubation, variable amounts of LH release were detected in the medium. This LH discharge, however, was not LHRH-dependent but proportional to the number of PT LH cells that were immunohistochemically detected in each incubated tissue. Since there was marked individual variation in the number of LH cells in the PT, the LH levels in the incubation medium were next compared before and after LHRH treatment using the same H-PT of the rat. An effect of LHRH could not clearly be shown in this experiment. Finally, the cytological response of the PT to LHRH was investigated by incubating both the H-PT and pituitary body connected to the intact pituitary stalk. Immunohistochemical examination of LHRH-treated tissues after 24 h revealed that, in females of both rats and mice, hormone depletion occurred in LH cells of the pars distalis but not in those of the PT. These results indicate that although LH cells in the PT can release LH in vitro, their mode of hormone synthesis and/or discharge differs from that of LH cells in the pars distalis. Since there was a marked individual variation and small LH-secreting capacity by the PT tissue, it seems unlikely, at least in rats and mice, that LH of PT origin plays an important role in the normal physiological state.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00213944
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    Electronic Resource
    Electronic Resource
    Protein absorption by tubular mesonephric and metanephric structures in the human embryo (1986)
    Springer
    Cell & tissue research 246 (1986), S. 77-80 
    Details
    ISSN: 1432-0878
    Keywords: Mesonephros ; Metanephros ; Protein reabsorption ; Immunohistochemistry ; Development, ontogenetic ; Man
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The presence of different serum proteins in the cells of the proximal tubule of both meso- and metanephric nephrons in human embryos (7th–12th week of intrauterine life) was investigated using immunohistochemistry. Endogenous lysozyme, alpha-1-antitrpysin and ferritin were detected in mesonephric proximal tubules and, starting from the 8th week, also in metanephric proximal tubules. Our observations provide information concerning the appearance and distribution of tubular protein reabsorption during the early stages of development.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00219002
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    Electronic Resource
    Electronic Resource
    Glucagon-related peptides in the rat hypothalamus (1986)
    Springer
    Cell & tissue research 246 (1986), S. 71-75 
    Details
    ISSN: 1432-0878
    Keywords: Glucagon-related peptides ; Rat hypothalamus ; Radioimmunochemistry ; Immunohistochemistry ; Food deprivation ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Immunohistochemically, nerve fibers and terminals reacting with anti-N-terminal-specific but not with anti-C-terminal-specific glucagon antiserum were observed in the following rat hypothalamic regions: paraventricular nucleus, supraoptic nucleus, anterior hypothalamus, arcuate nucleus, ventromedial hypothalamic nucleus and median eminence. Few fibers and terminals were demonstrated in the lateral hypothalamic area and dorsomedial hypothalamic nucleus. Radioimmunoassay data indicated that the concentration of gut glucagon-like immunoreactivity was higher in the ventromedial nucleus than in the lateral hypothalamic area. In food-deprived conditions, this concentration increased in both these parts. This was also verified in immunostained preparations in which a marked enhancement of gut glucagon-like immunoreactivity-containing fibers and terminals was observed in many hypothalamic regions. Several immunoreactive cell bodies were found in the ventromedial and arcuate nuclei of starved rats. Both biochemical and morphological data suggest that glucagon-related peptides may act as neurotransmitters or neuromodulators in the hypothalamus and may be involved in the central regulatory mechanism related to feeding behavior and energy metabolism.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00219001
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