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  • Immunocytochemistry  (121)
  • Yeast  (96)
  • Elasticity
  • Rheology
  • Springer  (224)
  • Air Force Geophysics Laboratory  (3)
  • Am. Geophys. Union  (3)
  • Gordon and Breach Sc. Publ.  (3)
  • Amsterdam ; New York : North-Holland  (1)
  • Inst. für Meteorologie und Geophysik der Johann Wolfgang von Goethe-Universität Frankfurt am Main  (1)
  • Blackwell Publishing Ltd
  • International Union of Crystallography
  • 1985-1989  (235)
  • 1975-1979
  • 1960-1964
  • 1988  (80)
  • 1987  (90)
  • 1986  (65)
Collection
Keywords
Publisher
Language
Years
  • 1985-1989  (235)
  • 1975-1979
  • 1960-1964
Year
  • 1
    Unknown
    Mathematical elasticity (1988)
    Amsterdam ; New York : North-Holland
    Details
    Keywords: DDC 531/.381 ; LC QA931 ; Elasticity
    Pages: Online-Ressource (3 v)
    ISBN: 9780444702593
    URL: http://www.sciencedirect.com/science/publication?issn=01682024&volume=20
    Language: English
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    E-BOOK
  • 2
    Electronic Resource
    Electronic Resource
    Thermotolerant single cell protein production byKluyveromyces marxianus var.marxianus (1988)
    Springer
    Journal of industrial microbiology and biotechnology 3 (1988), S. 9-14 
    Details
    ISSN: 1476-5535
    Keywords: Single cell protein ; Sucrose ; Yeast ; Thermotolerance ; Fermentation ; Kluyveromyces marxianus var.marxianus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Amino acid analyses were undertaken on single cell protein (SCP) produced by thermotolerant strains ofKluyveromyces marxianus var.marxianus grown on sugar cane molasses at 40°C. The maximum conversion of available sugars to biomass at 45°C was only 10.8% (g dry wt.·g−1 total sugars). The amino acid composition of the SCP did not differ markedly from that reported for other yeast species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01569436
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  • 3
    Electronic Resource
    Electronic Resource
    Rheological and thermal properties of polysaccharide gels extracted fromAhnfeltia plicata (1986)
    Springer
    Colloid & polymer science 264 (1986), S. 877-882 
    Details
    ISSN: 1435-1536
    Keywords: Rheology ; DSC ; gel ; polysaccharide ; Ahnfeltia plicata
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract The dynamic modulus began to decrease rapidly and loss tanδ showed maxima at the temperature range from 40 °C to 65 °C in polysaccharide gels extracted fromAhnfeltia plicata. An exothermic peak appeared at about 30 °C in heating DSC thermograms for these gels. Endothermic DSC peaks accompanying gel-sol transition appeared at the temperature range from 60 °C to 80 °C. The effects of alkali pretreatment and of maturation on the rheological and thermal properties of these gels were examined.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01410638
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  • 4
    Electronic Resource
    Electronic Resource
    Immunological homologies between yeast ribosomal protein L2 and rat liver ribosomal proteins L4 and L24 (1988)
    Springer
    Current genetics 13 (1988), S. 235-239 
    Details
    ISSN: 1432-0983
    Keywords: Ribosomal protein ; Immunological homology ; Yeast ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Polyclonal antibodies raised against ribosomal protein (r-protein) L2 of Schizosaccharomyces pombe were used to check for cross-reaktions with total r-proteins of rat liver. Using this procedure, the rat liver r-proteins, L4 and L24, were identified as being immunologically related to yeast L2. In addtional, homologies between rat liver L4 and L24 were detected. The possible implications for the regulation of r-protein synthesis are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00387769
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  • 5
    Electronic Resource
    Electronic Resource
    Plasmid associations with residual nuclear structures in Saccharomyces cerevisiae (1988)
    Springer
    Current genetics 13 (1988), S. 291-297 
    Details
    ISSN: 1432-0983
    Keywords: Yeast ; Nuclear matrix ; Plasmid stability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Acentric yeast plasmids are mitotically unstable, apparently because they cannot freely diffuse after replicating and therefore are not included in the daughter nucleus. This behavior could result if plasmids remain attached to structural elements of the nucleus after replicating. Since DNA replication is believed to take place on the nuclear matrix, we tested whether there was a correlation between the mitotic stability of a given plasmid and the extent to which it was found associated with residual nuclear structures. Residual nuclei were prepared from yeast nuclei by extraction with either high salt, 2 M NaCl, or low salt, 10 mM lithium diiodosalicylate (LIS). Hybridization analysis was used to estimate the fraction of plasmid molecules remaining after nuclei were extracted. We examined the extent of matrix association of three ARSI plasmids, Trpl-RI circle (1.45 kb), YRp7 (5.7 kb) and pXBAT (45.1 kb) with mitotic loss rates ranging from 3–25%. In addition we examined the matrix binding of the endogenous 2 μm plasmid and the 2 μm-derived YEp 13 which is relatively stable in the presence of 2 μm and less stable in cir° strains. Among the ARS1 plasmids we observed a negative correlation between stability and matrix association, consistent with models in which binding to the nuclear matrix prevents passive segregation of ARS1 plasmid molecules. No such correlation was observed among the 2 μn plasmids. Among all plasmids examined there is a positive correlation between size and matrix association.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00424422
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  • 6
    Electronic Resource
    Electronic Resource
    Cloning of the orotidine 5′-phosphate decarboxylase (ODC) gene of Schwanniomyces occidentalis by complementation of the ura3 mutation in S. cerevisiae (1988)
    Springer
    Current genetics 13 (1988), S. 29-35 
    Details
    ISSN: 1432-0983
    Keywords: Yeast ; Cloning ; ODC ; Complementation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A DNA fragment containing the gene encoding orotidine 5′-phosphate decarboxylase (ODC) from the yeast, Schwanniomyces occidentalis (formerly castellii) has been isolated from a genomic library constructed in the S. cerevisiae expression vector, pYcDE8. A recombinant plasmid, p2-lA, containing a 2.47 kb insert was shown to complement the ura3-52 mutation of several strains of S. cerevisiae. This DNA insert was shown to be from Schwanniomyces occidentalis by Southern hybridization analysis. A restriction enzyme cleavage map of the insert has been derived and the ODC gene localized to a 1.1 kb region by deletion analysis. In addition, we have demonstrated that expression of ODC is not dependent on the ADHI promoter carried on pYcDE8. This is the first report of the cloning of a gene from a member of the genus Schwanniomyces.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00365753
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  • 7
    Electronic Resource
    Electronic Resource
    The glucose- and ethanol-dependent regulation of PDC1 from Saccharomyces cerevisiae are controlled by two distinct promoter regions (1988)
    Springer
    Current genetics 14 (1988), S. 337-344 
    Details
    ISSN: 1432-0983
    Keywords: Yeast ; Gene regulation ; Saccharomyces cerevisiae ; PDCI promoter
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A 870 by promoter fragment of the PDC1 gene that includes the carbon source dependent regulatory regions was investigated using 5′ and 3′ promoter deletions. The results indicate that glucose and ethanol regulation of PDC1 transcription are independently controlled by distinct cis-acting regions. The consensus sequence AAATCGATA may play a role in this regulation, while the sequence (ATCA)AACCT may be important in transcription initiation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00419991
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  • 8
    Electronic Resource
    Electronic Resource
    Multiple elements regulate expression of the cell cycle-regulated thymidylate synthase gene of Saccharomyces cerevisiae (1988)
    Springer
    Current genetics 14 (1988), S. 363-373 
    Details
    ISSN: 1432-0983
    Keywords: Gene regulation ; Cell cycle ; Yeast
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Expression of the thymidylate synthase gene (TMPI) of Saccharomyces cerevisiae increases during the late G1 phase of the cell cycle. Using a series of gene fusions, which have placed the Escherichia coli lacZ gene under transcriptional and translational control of different portions of the TMPI gene, we have demonstrated the existence of three different regions which are important for expression. One of these regions, which was localized to within 270 base pairs of the translation start codon, is involved in the periodic expression of TMPI transcript. A second region, the deletion of which resulted in reduced levels of TMPI expression, is at least partially encoded by DNA sequences between 270 and 377 base pairs upstream of the translation start codon. A third region, located within the N-terminal 112 codons of the TMPI gene, apparently encodes information involved in a post-translational control mechanism.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00419994
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  • 9
    Electronic Resource
    Electronic Resource
    The cellular level of yeast ribosomal protein L25 is controlled principally by rapid degradation of excess protein (1986)
    Springer
    Current genetics 10 (1986), S. 733-739 
    Details
    ISSN: 1432-0983
    Keywords: Yeast ; Ribosome synthesis ; Regulation ; Ribosomal protein turnover
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary When the gene dosage for the primary rRNA-binding ribosomal protein L25 in yeast cells was raised about 50-fold, the level of mature L25 transcripts was found to increase almost proportionally. The plasmid-derived L25 transcripts were structurally indistinguishable from their genomic counterparts, freely entered polysomes in vivo and were fully translatable in a heterologous in vitro system. Nevertheless, pulse-labelling for periods varying from 3–20 min did not reveal a significant elevation of the intracellular level of L25 protein. When pulse-times were decreased to 10–45 s, however, we did detect a substantial over production of L25. We conclude that, despite the strong RNA-binding capacity of the protein, accumulation of L25 is not controlled by an autogenous (pre-)mRNA-targeted mechanism similar to that operating in bacteria, but rather by extremely rapid degradation of excess protein produced.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00405095
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  • 10
    Electronic Resource
    Electronic Resource
    Yeast centromere sequences do not confer mitotic stability on circular plasmids containing ARS elements of Tetrahymena thermophila rDNA (1987)
    Springer
    Current genetics 11 (1987), S. 353-357 
    Details
    ISSN: 1432-0983
    Keywords: Tetrahymena ; Replication ; Segregation ; Yeast
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We have previously demonstrated that a 657 bp TaqI-XbaI and a 427 by XbaI-XbaI fragment from the 5′ non-transcribed spacer of the extrachromosomal ribosomal DNA of Tetrahymena thermophila function as autonomously replicating sequences (ARS) in Saccharomyces cerevisiae. These fragments are adjacent to each other in a region that encompasses the in vivo origin of bidirectional replication of rDNA. The presence of a yeast centromere (CEN) fragment does not confer mitotic stability on these plasmids. A sensitive yeast colony colour assay (Hieter et al. 1985a) has been used to evaluate the cis-acting effect of each ARS segment on the pattern of inheritance of a plasmid containing CEN5:URA3:SUP4. Colonies of transformed cells obtained both in the presence and absence of selection were red with no detectable white or pink sectors. The lack of sectoring indicates that both plasmids are lost at an extremely high rate, likely due to 1:0 segregation events. We conclude that while these ARS elements confer a high frequency transformation phenotype, they lack a function which is required in cis for the maintenance of mitotic stability in the presence of a centromere. This missing cis-acting function may result in the inability of the plasmids to be brought under the control of cell-cycle regulated replication.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00378177
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