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  • Articles  (15)
  • Nodulation  (15)
  • 1980-1984  (15)
  • 1970-1974
  • 1925-1929
  • 1984  (15)
  • Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition  (15)
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  • Articles  (15)
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Years
  • 1980-1984  (15)
  • 1970-1974
  • 1925-1929
Year
Topic
  • Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition  (15)
  • Biology  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Plant and soil 79 (1984), S. 429-434 
    ISSN: 1573-5036
    Keywords: Alnus glutinosa ; Frankia ; Nodulation ; Soil acidity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Black alder seedlings were grown from seed for 7 weeks in six soils limed to various pH levels and inoculated withFrankia in two inoculation-seeding time combinations (inoculated and seeded concurrently; inoculated then seeded 5 weeks after inoculation). Three mine soils and three non-mine soils were used. Soil pHs in the study ranged from 3.6 to 7.6. In the second inoculation-seeding time combination, a series of soil samples at each of the pH levels below 7.0 were relimed to pH 7.0 immediately prior to seeding. The purpose of the study was to examine the effects of soil acidity on the nodulation of black alder byFrankia and the viability ofFrankia in acid soils. Based on the average number of nodules established per seedling, soil pH was determined to be a significant factor affecting nodulation in the mine soils. The highest levels of nodulation occurred between soil pH 5.5 and 7.2. Below pH 5.5, nodulation was reduced. There was also evidence of decreased viability of the endophyte below pH 4.5.
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  • 2
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    Plant and soil 77 (1984), S. 53-60 
    ISSN: 1573-5036
    Keywords: β-chloroethyltrimethylammonium chloride ; Gibberellins ; Nodulation ; Rhizobium japonicum ; Soya
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The effect of exogenous applications of gibberellins (GAs) or the growth retardant β-chloroethyltrimethylammonium chloride (CCC) on root nodule formation and activity (C2H2-reduction) in soya was studied. Daily foliar application of GA3 (2.89×10−6 M) delayed the formation of nodule initials and reduced the numbers mass nodule−1 and specific activity of nodules by 43%, 31% and 47% respectively, without affecting plant growth. Similar effects on nodulation were produced by foliar application of GA4 (3.01×10−5 M) or GA7 (3.03×10−5 M), or by the addition of GA3 (2.89×10−6 M) to the rooting medium. GA effectiveness in reducing nodule numbers was decreased by delaying its application until after the initial infection process had occurred, but the nodules formed were smaller and less active than those of the untreated control plants. The GA effect on nodulation and nodule activity was not associated with alterations in root exudate or due to a direct inhibitory effect of the hormone on the nitrogenase system. When the endogenous root content of GA-like substances was reduced (86% decrease) by foliar application of CCC (6.30×10−5 M), nodule numbers were increased by 56%, but nodule size and total nodule activity were similar to those of control plants. The GA and CCC treatments had no effect on rhizobial growth in liquid culture nor on root colonisation by rhizobia. The results suggest that the endogenous content of root GA may have a regulatory role in both the infection process and in subsequent nodule morphogenesis, thus controlling both the number and effectiveness of the root nodules formed.
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  • 3
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    Plant and soil 78 (1984), S. 171-179 
    ISSN: 1573-5036
    Keywords: Actinorhizae ; Alnus crispa ; Alnus viridis spp.crispa ; Clones ; Frankia ; Nodulation ; In vitro culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary 600,000 seedlings ofAlnus crispa were inoculated with a 1∶1∶1 mixture of theFrankia strains ACN1 AG , AGN1 exo AG and MGP10i. After 3 successive inoculations and screenings, one individual, AC-4, was selected as non-nodulating (Nod−) with Frankiae. This selected individual AC-4 (Nod−) and two other clones ofA. crispa, AC-2 and AC-5, known for their ability to nodulate (Nod+) and two other clones ofA. crispa, AC-2 and AC-5, known for their ability to nodulate (Nod+) withFrankia werein vitro propagated. The different clones ofA. crispa in culture required different kinds and concentrations of sugar during the in vitro multiplication and rooting stages. Nodulation tests using 7Frankia strains indicated that the clone AC-4 (Nod−) was non-nodulating with 6 of the 7Frankia strains tested. One strain,Frankia ANNI, isolated from one unique nodule produced on the mother-plant AC-4, induced 38% of the AC-4 plantlets to nodulate but with a number of nodules 10 to 20 times less than the clones AC-2 (Nod+) and AC-5 (Nod+). Morphological observations of the roots of AC-4 (Nod−) indicated that this clone had few and abnormally short root hairs.
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  • 4
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    Plant and soil 78 (1984), S. 367-379 
    ISSN: 1573-5036
    Keywords: Acidity ; Aluminium ; Calcium ; Manganese ; Nodulation ; pH Rhizobium ; Rhizosphere ; Root elongation ; Root hairs ; Trifolium repens ; White clover
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Effects of factors associated with soil acidity (low pH, low calcium, high aluminium and high manganese) on theTrifolium repens-Rhizobium trifolii symbiosis were investigted under laboratory conditions using an axenic solution-culture technique. 200 μM manganese increased root elongation in the range pH 4.3–5.5, but had no effect on root hair formation, the number of Rhizobium in the rhizosphere, or nodule formation. Root elongation and root hair formation were unaffected at pH 4.3 when 500 or 1000μM calcium was supplied, whereas multiplication of Rhizobium in the rhizosphere and nodulation were inhibited at pH 4.3 and 4.7.50–1000μM calcium had no effect either on the multiplication of Rhizobium in the range pH 4.3–5.5, or on nodule formation in the absence of aluminium. 50 μM aluminium inhibited, root elongation and root hair formation at pH 4.3 and 4.7; the effect on root elongation was reduced by increasing the calcium concentration from 50 to 1000μM. 50μM aluminium also inhibited Rhizobium multiplication in the rhizosphere and reduced nodule formation at pH 5.5 (at which aluminium precipitated out of solution), but root elongation and root hair formation were unaffected. These, effects of aluminium at pH 5.5 may explain the poor response to inoculation by white clover in acid mineral soils after liming.
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  • 5
    ISSN: 1573-5036
    Keywords: Hill soils ; Lime ; Mycorrhiza ; Nitrogen fixation ; Nodulation ; Phosphorus ; Rhizobium ; Symbioses ; White clover
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The responses of white clover (cv NZ Grasslands Huia grown in four UK hill soil types) to additions of lime and P, to inoculation with Rhizobium and mycorrhizal fungi, and to differences in soil water status were assessed in pot and field experiments. With a deep peat soil in pots, shoot production, nodulation and N fixation by clover were increased by 160, 130 and 85% respectively following inoculation with mycorrhiza, but in the field, despite a doubling of root infection, there was no response in growth. On a brown earth soil in the field inoculation with one endophyte (Glomus mosseae L1) out of four tested depressed production of white clover shoots by 42% but enhanced that of leeks (Allium porrum) by 50%; the others were without effect. With dry peaty podzol and brown earth soils in pots, clover shoot production was highest with added P when a water holding capacity of 80% was maintained, but roots from the latter had only 2.6 compared to 68 nodules per plant from the former. Further work is required to explain poor nodulation in the brown earth soils.
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  • 6
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    Plant and soil 78 (1984), S. 381-391 
    ISSN: 1573-5036
    Keywords: Aluminium ; Caloium ; Nodulation ; pH Phosphate ; Polymeric hydrolysis ; Rhizobium ; Rhizosphere ; Root elongation ; Root hairs ; Trifolium repens ; White clover
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Effects of aluminium on theTrifolium repens var Huia-Rhizobium trifolii strain HP3 symbiosis were studied using an axenic solution-culture system. With, 10 μM phosphate, 50 μM aluminium reduced or inhibited root elongation at pH〈5.0, root hair formation at pH〈 5.0–5.5, and Rhizobium multiplication in the rhizosphere and nodule formation at pH〈6.0. In the absence of aluminium, root elongation and root hair formation were reduced at pH〈4.3, and Rhizobium multiplication and nodule formation were inhibited at pH〈5.0. Root hair formation was more sensitive to aluminium at pH〈5 than was root elongation. No effect of aluminium on Rhizobium multiplication and nodule formation at pH〈5 was detected because both were sensitive to pH alone. At pH 5.5 most of the aluminium changed immediately to a form which was susceptible to low-speed centrifugation, but which was detected by the aluminon method of analysis, and after 24 h a precipitate formed. the concentration of phosphate was reduced also, to approximately 1μM. Toxicity was overcome by either increasing the phosphate concentration from 10 to 50 μM, or by increasing the pH to 6.0 and the calcium, concentration to 1000μM.
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  • 7
    ISSN: 1573-5036
    Keywords: Glycine max ; Inoculation ; IW/CPE ratio ; Nitrogen accumulation ; Nitrogen harvest ; Nitrogen re-distribution ; Nodulation ; Rhizobium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A field experiment was conducted on soybean (Glycine max (L.) Merrill) with a view to find out the effect of seed inoculation and scheduling of irrigation on nodulation, accumulation and re-distribution of nitrogen in plant tops and soil. The eight treatment combinations consists of two seed inoculations,viz. uninoculated and inoculated with rhizobium culture, and four irrigation schedules,viz. irrigation water to the cumulative pan evaporation (IW/CPE) ratio of 0.5, 0.7, 0.9 and a control (rainfed). Seed inoculation by, rhizobium culture increased the number, dry-weight and N content of nodules per plant. Inoculation of seeds also increased the N accumulation rate in plant top and it was 2.48 kg/ha/day during the flower-initiation to the pod-initiation stage (30–60 days interval). At harvest, 32.2, 47.8 and 26.2 kg N/ha was re-distributed from the stems, leaves and pods-wall of inoculated plants to the grains, respectively. A total of 186.5 kg N/ha was harvested and 64.7 kg N/ha, was accumulated in soil under the inoculated condition. Scheduling of irrigation at 0.7 IW/CPE proved better, than other irrigation schedules and helped in increasing the nodulation, nitrogen accumulation and grain yield. As compared to control, 8.4, 17.8 and 18.4 kg more of N/ha was redistributed from the stems, leaves and pods-wall respectively when the irrigations were scheduled at 0.7 IW/CPE ratio. Under this irrigation schedule the total N harvest was 200.1 kg/ha while the total N increased by 55.9 kg over that present in soil at the time of sowing.
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  • 8
    ISSN: 1573-5036
    Keywords: Acetylene reduction ; Cicer arietinum ; Chickpea ; N fixation ; Nodulation ; Root growth ; Shoot growth ; Soil nitrate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A controlled environment experiment was used to examine the growth and nodulation response of chickpea to a range of soil nitrate (NO 3 − ) levels (0, 0.75, 1.5, 3.0, 6.0 mM). Dry matter production, nodule mass and number, dinitrogen (N2) fixation via the acetylene (C2H2) reduction method, total nitrogen content and NO 3 − concentration were measured at 56 and 90 days from inoculated and uninoculated plants. It was found that chickpeas were less sensitive to the inhibitory effects of NO 3 − than soybeans. High NO 3 − appeared to inhibit the production of nodules early in growth, however, by the second harvest nodulation was stimulated by high NO 3 − levels. Increasing NO 3 − levels gave positive responses in tops and roots dry weight production but, proportionally, these effects were greatest with uninoculated plants. 3 and 6 mM NO 3 − gave similar root and tops dry weight in inoculated plants after 90 days. Nodule dry weight production per pot was maximised at 3.0 mM NO 3 − at both plant harvests. Whilst NO 3 − at 6 mM still gave a strong stimulation of acetylene reduction compared to 0 and 0.75 mM NO 3 − , there appeared to be a trend suggesting an inhibitory effect of 6 mM NO 3 − on C2H2 reduction compared to 1.5 and 3.0 mM NO 3 − .
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  • 9
    Electronic Resource
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    Plant and soil 82 (1984), S. 377-386 
    ISSN: 1573-5036
    Keywords: Centrosema ; Desmodium ; Inoculation ; Nitrogen yield ; Nodulation ; Oxisol ; Pueraria ; Rhizobium ; Soil cores ; Stylosanthes ; Tropical forage legumes ; Zornia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Three phases of Rhizobium inoculation trials were carried out as part of a programme to select forage legume germplasm adapted to acid, infertile Oxisols of tropical America. Firstly, a range of tropical forage legumes were evaluated for their response to N fertilization or inoculation with strains previously shown to be effective in Leonard jars, using cores of undisturbed soil or in the field at Carimagua, Meta, Colombia. In pure legume stands onlyCentrosema macrocarpum andC. pubescens showed increases in N yield due to both inoculation and N fertilization;C. brasilianum responded only to N fertilization;Zornia latifolia, Z. brasiliensis andStylosanthes capitata responded to neither treatment. Trials in cores and in grass-legume mixtures showed responses ofDesmodium ovalifolium, Pueraria phaseoloides andS. capitata to N fertilization but not to inoculation. In the second phase of experiments strains were screened in soil cores with 16 ecotypes ofDesmodium, Centrosema, Stylosanthes andPueraria spp. Significant increases in N yield due to inoculation occurred with at least one strain in all the legumes exceptS. guianensis ‘tardio’, and in some trials withS. capitata. In the third phase of trials the most effective strains were tested in the field. Significant response ofP. phaseoloides andC. macrocarpum to inoculation at two sites and in the second year after establishment were shown. Further screening trials and field trials at different sites are needed in order to provide better recommendations for inoculation of grazing trials being set up in the region under study.
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  • 10
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    Plant and soil 82 (1984), S. 415-425 
    ISSN: 1573-5036
    Keywords: Host-genetic control ; Nodulation ; Pisum sativum L. ; Rhizobium leguminosarum ; Symbiotic nitrogen fixation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Using primitive and wild pea plants from Afghanistan, Iran and Turkey, three host genes were detected, which confer resistance to nodulation by Rhizobium strains of cultivated peas from Europe. A dominant gene Sym 1 controls temperature-sensitive nodulation in pea cv. Iran. Another gene Sym 2 confers general resistance to a large number of European Rhizobium strains at all temperatures used. The degree of dominance of the latter gene is dependent on the Rhizobium strain used. A third gene Sym 4 is responsible for specific resistance to a single Rhizobium strain.
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  • 11
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    Plant and soil 82 (1984), S. 427-438 
    ISSN: 1573-5036
    Keywords: Mutant ; Mutation ; Nitrate ; Nitrate reductase ; Nodulation ; Pisum sativum L. ; Rhizobium leguminosarum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary In pea (Pisum sativum L.), mutants could be induced, modified in the symbiotic interaction withRhizobium leguminosarum. Among 250 M2-families, two nodulation resistant mutants (K5 and K9) were obtained. In mutant K5 the nodulation resistance was monogenic recessive and not Rhizobium strain specific. Out of 220 M2-families one mutant nod3 was found which could form nodules at high nitrate concentrations (15 mM KNO3). This mutant nodulated abundantly with severalRhizobium strains, both in the absence and presence of nitrate. Probably as the result of a pleiotropic effect, its root morphology was also changed. Among 1800 M2-families, five nitrate reductase deficient mutants were obtained and one of them (mutant E1) was used to study the inhibitory effect of nitrate on nodulation and nitrogen fixation. The results of the present investigation show that pea mutants which are modified in their symbiosis withRhizobium leguminosarum, can readily be obtained. The significance of such mutants for fundamental studies of the legume-Rhizobium symbiosis and for applications in plant breeding is discussed.
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  • 12
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    Plant and soil 82 (1984), S. 315-327 
    ISSN: 1573-5036
    Keywords: Breeding ; Competition ; Glycine max ; Nitrogen fixation ; Nodulation ; Phaseolus vulgaris
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary While symbiotic nitrogen (N2) fixation byG. max andP. vulgaris reduces their need for combined N, N2 fixation under field conditions is rarely maximized. This paper reviews constraints to N2 fixation in these species, then examines the genetic variability recorded for traits affecting N2 fixation and the further work needed in this area. It considers emerging programs for the improvement of N2 fixation inG. max andP. vulgaris and pays particular attention to methodological considerations.
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  • 13
    ISSN: 1573-5036
    Keywords: Abscisic acid ; Gibberellin ; Polyethylene glycol ; Rhizobium ; Roots ; Nitrogenase ; Nodulation ; Soya ; Water stress
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The effect of 2-day cycles of osmotically induced leaf moisture stress followed by partial recovery on the nodulation and nitrogenase activity of 2 soya cultivars was studied. Fourteen days after plant inoculation (mid-growth stage) the total leaf electrochemical water potential (ψwleaf) of control plants ranged from −0.8 to −1.9 bars, whereas the concentrations of osmoticum (polyethylene glycol 4000) induced ψwleaf values ranging from −1.4 (recovery value) to −3.1 bars (low stress), −1.8 to −4.4 bars (mild stress), and −2.2 to −6.2 bars (medium stress). The low stress treatment reduced nodule numbers and their specific activity in both cultivars, without affecting nodule size or the time required for nodule initiation. Nodule initiation was delayed in both cultivars by the mild and medium stress treatments, the former treatment reducing the number and size of the nodules and such nodules exhibited very low specific activity. The medium stress treatment prevented the further development of nodule initials, which remained inactive throughout the experiment. Such results imply an effect of water stress on the infection process and on nodule morphogenesis. The reduction in nodule numbers observed in water stressed plants was not associated with a reduced number of rhizobia in the rhizoplane nor due to an effect on root growth or root hair formation. At a stage prior to the formation of macroscopic nodule initials, the roots of plants under medium stress (ψwleaf=−5.5 bar)s) had a higher content of abscisic acid (ABA) (4-fold increase) and a lower content of gibberellin (GA)-like substances (21.4% reduction) as compared to control plants (ψwleaf=−1.0 bar). Although the medium stress treatment slightly increased the stomatal resistance of leaves, photosynthetic and transpiration rates were unaffected. Similar alterations of the hormononal balance occurred in the nodulated roots of plants subjected to naturally induced leaf moisture stress. Since the foliar application of ABA (1.92×10−5 M) to unstressed plants inhibited nodulation (45% reduction in nodule numbers), the increased endogenous content of thishormone in the roots of plants under leaf moisture stress may provide some physiological insight into the inhibitory effect of water stress on the nodulation process.
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  • 14
    ISSN: 1573-5036
    Keywords: Breeding ; Glycine max ; Nodulation ; Rhizobium spp. ; Soybean
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Most soybean varieties fail to nodulate effectively in tropical soils unless inoculated with a competitive strain ofRhizobium japonicum. Developing countries in the tropics, with few exceptions, lack inoculant industries to produce and distribute viable inoculants to small farmers and extension programs to teach them to use inoculant. Several soybean genotypes have been identified that nodulate effectively with many strains of the “cowpea” inoculation group which is ubiquitous in tropical soils of Africa. Soybean genotypes that nodulate and grow well without inoculant application are called “promiscuous”. Methodologies for incorporation of the promiscuity character into high-yielding backgrounds are discussed.
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  • 15
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    Plant and soil 80 (1984), S. 297-300 
    ISSN: 1573-5036
    Keywords: Nodulation ; Non-nodulating soybean ; Rhizobitoxine ; Rhizobium ; Soybean
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A significant increase in nodulation of “non-nodulating” (rj1 rj1) soybeans was obtained by inoculating with very high numbers (approx. 1011 cells/pot) of certain rhizobia when compared with inoculation at a moderate dose (approx. 109 cells/pot). Nodulating ability of rhizobial strains was not correlated with their ability to produce a detectable level of chlorosis-inducing toxin in culture.
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