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  • Drosophila melanogaster
  • Springer  (12)
  • Cambridge University Press
  • Frontiers Media
  • Molecular Diversity Preservation International
  • 2020-2022
  • 1980-1984  (12)
  • 1975-1979
  • 1984  (12)
Collection
Publisher
  • Springer  (12)
  • Cambridge University Press
  • Frontiers Media
  • Molecular Diversity Preservation International
  • Wiley-Blackwell  (1)
Years
  • 2020-2022
  • 1980-1984  (12)
  • 1975-1979
Year
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Cellular and molecular life sciences 40 (1984), S. 1396-1397 
    ISSN: 1420-9071
    Keywords: Drosophila melanogaster ; ovicidal activity ; dehydromatricaria ester ; toxicity, enhancement by UV ; polyacetylenic compounds
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Freshly laid eggs ofDrosophila melanogaster were treated with the polyacetylenic compoundcis-dehydromatricaria ester. The toxicity of the chemical was enhanced by treatments with long-wavelength ultraviolet light, and the maximum effect was observed when the UV irradiation was performed 4–5 h after the initial contact of the eggs with the chemical.
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Development genes and evolution 193 (1984), S. 133-138 
    ISSN: 1432-041X
    Keywords: Drosophila melanogaster ; Compartment boundary ; Operculum seam
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Histochemical staining of the hypoderm ofDrosophila larvae for aldehyde-oxidase activity allowed detection of a row of cells destined to form the operculum seam, along which the pupal case opens when the adult ecloses. Analysis ofmal clones in hypoderms of gynandromorph larvae showed that the prospective operculum seam coincides, in part, with a line of clonal restriction that divides the thorax into dorsal and ventral halves. We propose that this line represents the embryonic dorsal/ventral compartment boundary
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Development genes and evolution 193 (1984), S. 226-233 
    ISSN: 1432-041X
    Keywords: Wing development ; Sensory neurons ; Differentiation ; Axonal outgrowth ; Drosophila melanogaster
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary TheDrosophila wing is a simple structure bearing many sensory neurons which form an elementary pattern of nerves within the veins, and provides a simple system for studying the formation of nerve pathways. A light-and electron-microscope study of the developing pupal wing was undertaken to establish the time at which the different classes of sensillum differentiate and to determine the arrangement of tissues within the wing during sensory axon out-growth in order to assess possible candidates for directing axon outgrowth. Major findings were 1. Wing development passes through three main stages: at 6–12 h after puparium formation the wing is flantened and secreting pupal cuticle; at 15–18 h the wing is dramatically inflated but is still a simple epithelium secreting pupal cuticle; at 21–24 h the wing collapses and begins to differentiate adult structures. 2. There are no persisting larval nerves which might act as pioneers or pathfinders for the later developing adult neurons. 3. Axon bundles are first observed during the 15–18 h stage prior to the pupal moult when the wing is still secreting pupal cuticle. 4. At this stage the wing is an inflated sac, without any veins or orderly arrangements of tracheae which might act as guides for axon outgrowth. Vein formation takes placeafter formation of the axon pathways. 5. The bristle axons grow along the anterior wing margin in close contact with the basal lamina of the epithelial cells, often within a gap between the processes of the epithelial cells, which could mechanically channel their out-growth. 6. The campaniform sensillum axons appear to navigate along the inner surface of the wing epithelium rather like the pioneer axons found in embryonic appendages of other insects. 7. Differentiation of sensory neurons takes place long before differentiation of the other cells associated with sensilla; most axons are present by 18 h when the wing is still secreting pupal cuticle but morphological differentiation of the bristle shaft and socket cells is not observed until about 42 h when the wing is secreting the cuticulin layer of the adult cuticle.
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Development genes and evolution 193 (1984), S. 242-245 
    ISSN: 1432-041X
    Keywords: Chaetae ; Sensillae ; Differentiation ; Genetic regulation ; Drosophila melanogaster
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Mutants in two loci,hairy (h +) andextramacrochaetae (emc +), produce phenotypes corresponding to an excess of function of theachaete-scute complex (AS-C), that is, they cause the appearance of extra chaetae. These mutants, although recessive in normal flies, become dominant in the presence of extra doses of AS-C. Here we study the interactions between these three genes, in an attempt to elucidate their relationships. The results show that the insufficiency produced byh oremc mutants can be titrated by altering the number of copies of AS-C. Moreover, excess of function of AS-C produced by derepression mutants within the complex (Hairy-wing) can also be titrated by altering the number of wild type copies of+ oremc +. These specific interactions indicate that bothh + andemc + code for “repressors” of AS-C that interact with theachaete andscute region of the complex respectively.
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  • 5
    Electronic Resource
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    Springer
    Development genes and evolution 193 (1984), S. 246-251 
    ISSN: 1432-041X
    Keywords: Chaetae ; Differentiation ; Genetic regulation ; Pattern formation ; Drosophila melanogaster
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We have already shown that theachaetae-scute complex (AS-C) ofDrosophila is regulated by two genes,hairy andextramacrochaetae. Using mutants in these genes, we have analysed how different levels of expression of AS-C affect the pattern of chaetae. The results indicate that the spatial distribution of chaetae results from cell interactions, probably by a mechanism of lateral inhibition. The results are discussed in view of the different theories of pattern formation.
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  • 6
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    Springer
    Biochemical genetics 22 (1984), S. 551-566 
    ISSN: 1573-4927
    Keywords: Drosophila melanogaster ; larval serum protein polymorphisms ; genetic heterogeneity ; northern populations
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract LSP-1βS is present in Michigan and Massachusetts Drosophila melanogaster natural populations. Its frequency, 10%, is significantly higher in an East Jordan, Mich. (latitude, 45.10° N), population than in East Lansing, Mich. (latitude 42.44° N), or Hadley, Mass. (latitude, 42.21° N), populations, where it averages 3% at each location. The average frequency of LSP-2S is more comparable, 6, 5, and 7% at East Jordan, East Lansing, and Hadley, respectively. LSP-1γF variants are also present. A total of 342 single third-instar larvae was scored for LSP-1 autosomal variants, and 323 for LSP-2 variants. Each larva represented a newly established isofemale line from collections at East Jordan in 1981 and 1983, East Lansing in 1982, and Hadley in 1981, 1982, and 1983. Within localities, frequencies of hemolymph protein variants did not differ significantly between years. Proteins 9, 10, 11, and 15 correspond to the LSP-1γ, β, and α triplet and LSP-2 polypeptide in D. melanogaster. Our results together with those of Singh and Coulthart [(1982). Genetics 102:437] indicate that D. melanogaster populations in north temperate climates maintain considerable genetic heterogeneity for the larval hemolymph proteins.
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  • 7
    ISSN: 1573-4927
    Keywords: larval serum protein ; gene dosage ; Drosophila melanogaster
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Larval serum protein-1 (LSP-1) and LSP-2 are the major proteins of Drosophila larval serum. The amount of LSP-1 synthesized is strictly proportional to the number of LSP-1 genes present within the range 1–10. The normal number in female flies is 6. Flies with extreme amounts of LSP-1 were, by our criteria, as fit as the wild type. The ratio of LSP-2:LSP-1 was analyzed in 169 different stocks and was constant in 164 of these. The significantly different ratios in five stocks were all due to the lack of one of the LSP-1 gene products.
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  • 8
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    Springer
    Biochemical genetics 22 (1984), S. 453-465 
    ISSN: 1573-4927
    Keywords: Drosophila melanogaster ; ribosomal DNA ; compensation ; selective amplification
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Compensation is a mechanism by which the X-chromosome nucleolus organizer region of Drosophila melanogaster can increase its ribosomal DNA content up to twofold. It occurs in somatic cells under specific genetic conditions and is mediated by a defined genetic site, the compensatory response locus. The In and various type I ribosomal DNA repeat units were separated by restriction endonuclease digestion. Comparison of the percentages of these repeat unit types between compensating and noncompensating genotypes showed the same distribution. Therefore no selective amplification of these repeat unit types occurs during ribosomal DNA compensation. These results demonstrate that two processes of rDNA amplification in somatic cells, compensation and independent rDNA polytenization, are exclusive events.
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  • 9
    ISSN: 1573-3297
    Keywords: sympatric populations ; Drosophila melanogaster ; Drosophila simulans ; olfaction ; alcohols ; methanol ; ethanol ; propanol ; iso-propanol ; butanol ; hexanol ; octanol ; “threshold response model.”
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Psychology
    Notes: Abstract Alcohol preference by adults (1–2 days old) of sympatric populations ofDrosophila melanogaster andD. simulans were studied in a T-shaped glass maze. Concentrations for ethanol, methanol, propanol, and hexanol alcohols (group I) were 10, 25, 35, and 50%; for iso-propanol, butanol, and octanol alcohols (group II), they were 1.5, 2.5, 3.5, and 10%.D. simulans preferred the four alcohols in group I, more thanD. melanogaster. While moreD. melanogaster thanD. simulans preferred iso-propanol, moreD. simulans thanD. melanogaster preferred butanol. The two species had a similar preference for octanol. Regardless of the position of alcohol in the maze,D. melanogaster preferred to turn right in the presence of all alcohols, whileD. simulans preferred to turn right in the presence of group I alcohols only. At 10% concentration, males of both species preferred to turn right and females preferred to turn left. More females than males ofD. simulans preferred group II alcohols. There was no significant difference among concentrations for all alcohols in group I forD. melanogaster. Highly significant differences among concentrations were detected for group I alcohols forD. simulans and group II alcohols for both species.D. simulans was more dependent on ethanol concentration thanD. melanogaster. There was also an alcohol by concentration interaction forD. simulans group I andD. melanogaster group II. At the common concentration (10%) there was no difference between the two species in their preference for any alcohol, with the following ranking order in attractiveness: methanol〉ethanol〉propanol 〉hexanol〉octanol〉butanol〉iso-propanol. There was a negative correlation between some physical properties of alcohols and their attractiveness. In general, the results are consistent with the “threshold response model.”
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  • 10
    ISSN: 1573-3297
    Keywords: oviposition behavior ; circadian rhythm ; Drosophila melanogaster ; photoperiodism ; genetic drift
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Psychology
    Notes: Abstract Under photoperiodic conditions, flies recently collected in nature exhibit, at the beginning of the scotophase, an oviposition peak which has a higher amplitude in Afrotropical than in European temperate populations. Several old laboratory strains failed, however, to show this peak. In each cross between genetically different strains, the oviposition curves of F1 and F2 were usually close to the midparent curve. Ten isofemale lines from an Afrotropical populations were submitted to inbreeding and drift. After 100 generations, two of the four surviving lines had retained the high peak typical of the origin population while the two others had lost it. Chromosome substitutions between these lines demonstrated a polygenic inheritance with a significant effect of the three major chromosomes. Presumably, the variations of amplitude of the oviposition peak were not caused by a modification of the ovarian activity but by a behavioral change toward the external signals of the environment.
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  • 11
    Electronic Resource
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    Springer
    Behavior genetics 14 (1984), S. 269-277 
    ISSN: 1573-3297
    Keywords: negative phototaxis ; artificial selection ; dispersal behavior ; habitat choice ; Drosophila melanogaster
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Psychology
    Notes: Abstract Population dispersal ofDrosophila melanogaster in the wild has been studied with the release and recapture of 60,000 flies from two photonegative selection lines and their photopositive control. The results show evidence of phototaxis-dependent habitat choice of at least one photonegative population. For this reason, phototactic behavior measured in Y mazes could be of evolutionary significance inDrosophila melanogaster.
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  • 12
    Electronic Resource
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    Springer
    Cell & tissue research 236 (1984), S. 279-288 
    ISSN: 1432-0878
    Keywords: Nucleoli ; Fibrillar centres ; Chromatin ; Drosophila melanogaster
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In embryonic cell-line derivative KCo of Drosophila melanogaster, the nucleolus, like most nucleoli, contains a small proportion of ribosomal DNA (1–2% of the total nucleolar DNA). The ribosomal DNA is virtually the only active gene set in the nucleolus and is found among long stretches of inactive supercoiled heterochromatic segments. We have demonstrated by use of a Feulgen-like ammine-osmium staining procedure that, depending on the state of growth, more or less fibres of decondensed DNA emanating from the intra-nucleolar chromatin (which is in continuity with the nucleolus-associated chromatin) ramify and unravel within the central nucleolar core to be transcribed. The nucleolus expands or contracts with the variation of activity and could belong to a supramolecular matricial structure such as is shown after extraction of the nuclei. After a long period of exposure to high doses of actinomycin D, the central nucleolar core became an homogeneous fibrous structure that could be interpreted as an aggregate of protein skeletal elements. The mechanism of repression and derepression of the nucleolar chromatin could thus be explained by a mechanism involving in part a sub-nucleolar structure. We propose a schematic organization of the nucleolar chromatin in KCo cells of Drosophila and discuss it in relation with other nucleolar organizations.
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