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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Cellular and molecular life sciences 40 (1984), S. 1004-1006 
    ISSN: 1420-9071
    Keywords: Rat ; adrenocortical responsiveness ; ACTH ; plasma ; corticosterone ; plasma ; corticotropin releasing factor (CRF)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In the course of studying the plasma adrenocorticotropic hormone (ACTH) and corticosterone responses to synthetic corticotropin releasing factor (CRF), we noted some disparity in the responses. A higher dose (20 μg compared with 5 μg per rat i.a.) produced an equal plasma ACTH but greater plasma corticosterone response in adult male rats. Thus, we examined the possibility that CRF increases adrenocortical responsiveness to ACTH. CRF significantly (p〈0.0005) increased the plasma corticosterone response to ACTH in rats pretreated with dexamethasone. Thus, synthetic CRF increases corticosterone secretion in rats not only by stimulating ACTH secretion, but also by increasing the adrenocortical responsiveness to ACTH.
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  • 2
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    Cellular and molecular life sciences 40 (1984), S. 974-975 
    ISSN: 1420-9071
    Keywords: Rat ; prostaglandins ; gastric lesion ; intragastric distension model ; stress model ; indomethacin ; somatostatin preventive effect
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The inhibition of endogenous prostaglandin synthesis by indomethacin treatment blocks the somatostatin preventive effect on the gastric lesions induced in a stress model and has no preventive effect on an intragastric distension model.
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  • 3
    ISSN: 1420-9071
    Keywords: Rat ; cerebrospinal fluid, human ; analgesia ; naloxone ; pain indifference, congenital ; opiates, endogenous
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary CSF from a patient with congential indifference to pain was found to produce analgesia in the rat following intracerebroventricular injections. The analgesic effect was attenuated by pretreatment with naloxone suggesting the involvement of hyperactive endogenous opiate mechanisms in this patient.
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  • 4
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    Cellular and molecular life sciences 40 (1984), S. 1368-1369 
    ISSN: 1420-9071
    Keywords: Rat ; kidney ; hypertensive ; prostaglandin dehydrogenase ; hexokinase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary 15-Hydroxyprostaglandin dehydrogenase (PGDH) surged in hypertensive (SHR) and normotensive (WKY) rat kidney at 8 days of age, is greatest in SHR. Hexokinase fell in SHR at 17 days of age, but thereafter was similar to WKY. This suggests multisystem enzymatic abnormalities in SHR kidney during development of hypertension.
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  • 5
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    Cellular and molecular life sciences 40 (1984), S. 1008-1010 
    ISSN: 1420-9071
    Keywords: Rat ; ethanol preference ; acetaldehyde self-administration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Peripherally self-injected acetaldehyde in interaction with environmental and nutritional variables significantly enhances alcohol drinking in rats and suggests an involvement of acetaldehyde in voluntary alcohol intake.
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  • 6
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    Springer
    Cell & tissue research 236 (1984), S. 373-381 
    ISSN: 1432-0878
    Keywords: Merkel cell surface ; Quinacrine fluorescence ; Lectins ; Vibrissae ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Surface carbohydrates on the Merkel cell of the outer root sheath (ORS) were investigated in 1to 4-day-old rat vibrissae by use of rhodamine isothiocyanate (RITC)-conjugated lectins. The red fluorescence of RITC provided a convenient assay for lectin binding to the Merkel cell, which is itself identified by its green fluorescence following selective uptake of the dye quinacrine. In monolayers or suspensions of freshly dissociated ORS cells, the Merkel cell showed high affinity for the α-fucose-specific lectin, Ulex europeus agglutinin I (UEA-I), thus revealing a novel feature for a basally located cell. Other high-affinity lectins included concanavalin A (Con A), wheat germ agglutinin (WGA), soybean agglutinin (SBA), and Ricinus communis agglutinin I (RCA-I). In contrast, Dolichos biflorus (DBA), Bandeiraea simplicifolia I and II (BS-I and BS-II), and peanut agglutinin (PNA) virtually excluded the Merkel cell, though PNA-binding sites were unmasked after neuraminidase treatment. Other dispersed ORS cells had varying lectin affinities, and generally binding was inhibited by a competing haptenic sugar. The pattern of lectin binding seen in cryostat and paraffin sections of the vibrissa suggested that the Merkel cells share surface properties with their neighboring basal and/or spinous cells; however, unshared properties are likely to exist since ingrowing mechanosensory nerves recognize the Merkel cells, and not other epidermal cells, as their targets.
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  • 7
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    Cell & tissue research 236 (1984), S. 711-715 
    ISSN: 1432-0878
    Keywords: Gastric antral mucosa ; Caerulein ; Gastrointestinal hormones ; Cholecystokinin ; Trophic effect ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The growth-promoting effect of caerulein on antral gastric mucosa was explored using Wistar rats. Implanted osmotic minipumps were used to administer submaximal doses of either caerulein or saline to normal rats for up to 4 days. In one group, reflux of bile and pancreatic juice into the stomach was avoided by previous surgical diversion of the distal common bile duct to the jejunum. DNA synthetic and mitotic activity in the antrum epithelium were estimated by 3H-thymidine pulse labelling and autoradiography during the administration of the peptide. The rate of cell migration was determined in animals killed 1, 2 and 3 days after the 3H-thymidine pulse. Administration of caerulein to normal rats provoked significant increases in both labelling and mitotic indices, and a significant acceleration of the upward cell migration in the glandular tubes. In the animals with distal diversion of bile and pancreatic secretions both labelling and mitotic indices were also increased over control values under the effect of the peptide. These data indicate that administration of caerulein stimulates cell proliferation in the antral gastric mucosa. This effect cannot be explained through increased reflux of pancreaticobiliary secretions in the stomach.
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  • 8
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    Cell & tissue research 236 (1984), S. 699-709 
    ISSN: 1432-0878
    Keywords: Testis ; Spermatogenic cycle ; Sertoli cell ; Lipid ; Morphometry ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The volume and surface area of lipid inclusions often present in the cytoplasm of rat Sertoli cells was measured directly from semi-thin sections of perfusion-fixed testicular tissues using an image analyser linked to a light microscope. Sertoli cell nuclei were used as a reference for comparing any variations in the measured parameters of lipid inclusions during the rat spermatogenic cycle. Volume density of Sertoli cell lipid inclusions was assessed by morphometric analysis of Sertoli cells photographically reconstructed from electron micrographs. Maximum lipid content in Sertoli cells occurred during stages IX–XIV of the spermatogenic cycle, then declined at stages I–III and remained low from stages IV–VIII. The persistence and increase in number of many large Sertoli cell lipid inclusions beyond the stage where spermatid residual bodies are phagocytosed within the Sertoli cells (stage IX) suggests that the synthesis and lipolysis of Sertoli cell lipid inclusions represents an intrinsic functional cycle of the Sertoli cells. Stage-dependent variations in the lipid content of rat Sertoli cells offers morphological evidence that the metabolic duties of the Sertoli cells are synchronised with the spermatogenic cycle to provide local coordination of the proliferation and maturation of the germ cells.
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  • 9
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    Cell & tissue research 236 (1984), S. 717-724 
    ISSN: 1432-0878
    Keywords: Ovary ; Ovarian follicle ; Atresia ; Immunoregulation ; Immune tolerance ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Thy-1+ cells, producing Thy-1+ material, have been demonstrated by the indirect immunoperoxidase technique in the theca of growing ovarian follicles of the rat. OX-2 antigen, known as the minor glycoprotein of rat thymocytes, was detected in granulosa cells of non-growing follicles. Ia+ cells of dendritic type and/or activated macrophages were identified in the granulosa of advanced degenerating follicles, and remnants of the zona pellucida exhibited immunoglobulins. In some ovaries immunoglobulins were also bound to the zona pellucida of oocytes of early degenerating antral follicles. Medium-sized antral follicles with degenerating granulosa were occasionally invaded by cells carrying antigens of cytotoxic T lymphocytes or other T lymphocyte subsets, while degenerating large antral follicles were sometimes invaded by cells exhibiting antigen of cells with natural killer function (but not antigens of T lymphocytes). Granulosa cells of some degenerating antral follicles exhibited class-I antigens derived from the major histocompatibility complex. We suggest that cell-mediated control mechanisms of antigen expression and metabolism of tissue cells during their differentiation and degeneration should be considered in addition to the well-documented hormonal dependence of some tissues.
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  • 10
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    Cell & tissue research 237 (1984), S. 245-252 
    ISSN: 1432-0878
    Keywords: Pineal organ ; Interstitial cells ; Astrocytes ; Immunocytochemistry ; Rat ; Mouse
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Antigenic markers characteristic of astrocytes and their differentiative states (i.e., glial fibrillary acidic protein (GFAP), vimentin, and M1 and C1 antigens) were investigated in the pineal gland of mouse and rat using double immunolabeling techniques. In both species the socalled interstitial cells as characterized by TEM were shown to be astrocytes, since they expressed vimentin, but neither fibronectin (a marker for fibroblasts and endothelial cells) nor the neuron-specific L1 antigen or tetanus toxin receptors. Subpopulations of vimentin-positive pineal astrocytes were also GFAP- and C1- antigen-positive. M1- antigenpositive cells were not detected. It is concluded that a considerable proportion of interstitial cells in the pineal gland of rat and mouse are immature astrocytes which, in contrast to other parts of the central nervous system, persist into adulthood.
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  • 11
    ISSN: 1432-0878
    Keywords: Thyroid gland, fetal ; Cytoskeleton ; Cytocha lasin B ; Vinblastine ; Colchicine ; Follicular development (thyroid) ; Tissue culture ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Thyrotropic hormone (TSH) or cAMP accelerate the formation of follicular cavities in the explanted thyroid gland of the 15-day-old rat fetus. Cytochalasin B or vinblastine and nocodazole or colchicine, which disorganize microfilamental and microtubular structures respectively, inhibit or completely block in vitro-induced folliculogenesis. Exposure of the thyroid tissue to lumicolchicine, a structural isomer of colchicine deprived of antimicrotubular activity, does not inhibit the activation of folliculogenesis induced by TSH. These results are strong evidence for the supposition that microfilaments and microtubules are involved in the TSH-stimulated mechanisms resulting in thyroid folliculogenesis. Folliculogenesis requires the integrity of both microfilaments and microtubules.
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  • 12
    ISSN: 1432-0878
    Keywords: CRF-neurons ; Hypothalamus ; Development, ontogenetic ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Appearance of immunoreactive corticotropin-releasing factor (CRF)-containing neurons was studied in developing hypothalamus of the rat by use of antisera against rat- and ovine CRF. These neurons were first recognized in the lateral and paraventricular nuclei on days 15.5 and 16.5 of gestation, respectively, when antiserum against rat CRF was employed. Antiserum against ovine CRF revealed the cells two days later exclusively in the latter nucleus. In both nuclei, the neurons increased in number with development. The neurons in the paraventricular nucleus appeared to project their immunoreactive processes to the median eminence via the periventricular and lateral pathways. In the median eminence, the immunoreaction with antiserum to rat CRF was first recognized in its anterior portion in the form of dots on day 16.5 of gestation but as beaded fibers in the external layer on day 17.5; these structures increased in amount with development in rostro-caudal direction. Although antiserum to ovine CRF was less potent in immunostainability than antiserum to rat CRF, it also revealed the beaded fibers in the median eminence on day 17.5 of gestation. Since evidence is available that the paraventricular nucleus is involved in corticotropin release, it is concluded that, in rats, the hypothalamic regulatory mechanism controlling the release of corticotropin initially appears on days 16.5–17.5 of gestation.
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  • 13
    ISSN: 1432-0878
    Keywords: Caldesmon ; Actin ; Immunocytochemistry ; Small intestine ; Smooth muscle ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The distribution of caldesmon (a calmodulin-binding, F-actin-interacting protein) (Sobue et al. 1982) and of actin was studied in the rat's small intestine by means of light-microscopic immunocytochemistry. Positive immunostaining for caldesmon was seen in smooth muscle cells of the intestinal wall, and of blood vessels, and in the apical portion of the absorptive epithelial cells. The immunoreactivity in goblet cells was difficult to recognize. The positive reaction to immunostaining for actin showed almost the same pattern as that for caldesmon. These results suggest that this calmodulin-binding protein may play an important role in the control of actin-myosin interaction in smooth muscle cells and in non-muscle cells.
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  • 14
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    Cell & tissue research 235 (1984), S. 433-438 
    ISSN: 1432-0878
    Keywords: Mammary gland ; Ferritin-concanavalin A ; Concanavalin A ; Endocytosis ; Membrane reuse ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Ferritin-concanavalin A (Fer-Con A) was used to label the apical plasma membrane of the lactating cell to determine whether membrane internalization takes place. Rat glands were infused in vivo via the teat with 0.2 mg of Fer-Con A in 0.2 ml tris buffer (pH 7.0) containing 0.1% trypan blue, the latter acting as a marker of the infusate. Tissues were obtained from separate animals 5, 10 and 60 min postinfusion. Fer-Con A was seen in alveolar lumina bound to the outer surfaces of apical plasma membrane, microvilli and milk fat globules. It was observed within lactating cells on the inner membrane surfaces of endocytotic vesicles, Golgi cisternae, and secretory vesicles containing casein micelles, and in multivesicular bodies and lysosomes. Internalization of the ferritin-lectin conjugate into casein-containing secretory vesicles was detectable in the 5-min postinfusion tissue. Lysosomes were the only structures in control tissue that contained particles bearing some resemblance to Fer-Con A. The data provide evidence that apical plasma membrane is internalized and distributed to a number of intracellular compartments.
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  • 15
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    Cell & tissue research 235 (1984), S. 449-452 
    ISSN: 1432-0878
    Keywords: Suprachiasmatic nucleus ; Morphometry ; Synapses ; Sexual dimorphism ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The suprachiasmatic nucleus (SCN) of male rats was estimated to contain 16×106 synaptic appositions (unilaterally) or 250×106 appositions in 1 mm3 tissue of the nucleus with an average of 1404 appositions per neuron. There are significantly fewer synaptic appositions in the suprachiasmatic nucleus of female rats (15×106 per SCN or 236×106 in 1 mm3 tissue of SCN with 1264 appositions per neuron on an average). Additionally, numbers of various types of synapses (axo-somatic, invaginated, dendrodendritic and optic) are estimated.
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  • 16
    ISSN: 1432-0878
    Keywords: Monosodium-1-glutamate ; Neuropathology ; Rat ; Superior colliculus ; Toxicology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Systemic administration of monosodium-1-gluta-mate by single injections of 4 mg/g body weight in infant rats (2–10 days of age) results in acute swelling of cytoplasm and nuclear pyknosis of neurons in the stratum zonale and stratum griseum superficiale of the superior colliculus. Multiple daily doses of 4 mg/g body weight monosodium-1-glutamate result in an almost complete loss of neurons in these two superficial layers. The deeper layers appear not to be affected. No pathological effects were observed in the lateral geniculate body or pretectal complex. Light-and electron-microscopic studies reveal that the optic nerves are remarkably shrunken and many myelinated as well as unmyelinated axons are lost. Injection of 3Hproline into the vitreous body of one eye results in limited transport to the suprachiasmatic nucleus, lateral geniculate body and to lateral portions of the superior colliculus. The small percentage of intact axons in the optic nerve, as well as the limited proline transport from the eye, suggest that administration of monosodium-1-glutamate leaves intact some optic fibers, a portion of which belongs to the retinohypothalamic tract.
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  • 17
    ISSN: 1432-0878
    Keywords: Exocrine pancreas ; Calcium pool ; Calcium release ; Electron microscopy ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In an attempt to identify a cellular Ca2+-pool, from which calcium is released when secretagogues are applied, tissue fragments of the rat exocrine pancreas were incubated and fixed with glutaraldehyde in the presence of calcium. By means of this procedure electron-dense deposits were found on plasma membranes. X-ray microanalysis showed that these deposits contain calcium. Stimulation of tissue fragments with the use of the secretagogues carbachol or cholecystokinin reduced the number of deposits by about 80%. When the antagonist atropine was applied after carbachol stimulation, deposits reappeared on cell membranes, which then disappeared again after a second stimulation with cholecystokinin. In the presence of procaine, carbachol was inhibited and only slightly reduced the Ca2+-deposits on the plasma membranes. These results suggest that a calcium pool, from which calcium is released to induce enzyme secretion on stimulation, is located in the cell membrane
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  • 18
    ISSN: 1432-0878
    Keywords: Somatostatin ; Ontogenesis ; Electron-microscopic immunohistochemistry ; Median eminence ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The development of immunoreactive (ir) somatostatin-containing nerve terminals in the rat median eminence (ME) has been examined electron-microscopically. Nerve fibers containing ir particles scattered throughout the axoplasm are first seen in the external layer of the ME on day 18.5 of gestation, and, on day 21.5 appear to terminate on the basement membrane of the perivascular space of the portal vessels. After birth, the fiber terminals contain several membrane-limited granules, which are labeled with ir PAP particles. Ultrathin, Epon-embedded sections of ME, treated by the protein A gold-labeling method for somatostatin, demonstrate positively labeled granules in the nerve fibers in the postnatal ME, but in the prenatal tissue, no specific gold-labeling is found. These findings show that, in the external layer of the ME, somatostatin storing occurs in the granules in the axonal terminals after birth.
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  • 19
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    Cell & tissue research 236 (1984), S. 491-493 
    ISSN: 1432-0878
    Keywords: Brain vessels ; Basal lamina ; Pericytes ; Endothelial cells ; Glial cells ; Argyrophilic staining ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Vibratome sections obtained from perfusion-fixed rat brains were stained by means of silver impregnation and physical development according to Gailyas (1970). Small pieces of the cerebral cortex were postfixed with buffered osmium tetroxide solution and processed for electron microscopy to examine the localization of the silver deposit at the cellular level. The cell surfaces of pericytes and smooth muscle cells were completely outlined by silver grains. Endothelial cells and perivascular astrocytes, however, showed an asymmetric distribution of the silver deposit, i.e., the deposit was restricted to the abluminal endothelial surface and to the astrocytic membrane adjacent to the vessel wall, respectively. The method allowed a clear-cut distinction between perikarya of endothelial cells and pericytes as well as glial cells in perivascular position, even at the light-microscopic level.
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  • 20
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    Cell & tissue research 236 (1984), S. 249-255 
    ISSN: 1432-0878
    Keywords: Oocyte ; Nucleolus ; Silver staining ; Electron microscopy ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The nucleoli of dictyate-stage growing oocytes in rat ovaries were examined both with routine electron microscopy and electron microscopy after silver nitrate and ammoniacal silver nitrate (Ag-AS) staining. The nucleoli of the unilaminar follicular oocytes consist of twisted strands of dense fibrillar components, aggregates of granular components, and small fibrillar centers. After Ag-AS staining, silver grains are numerous on the dense fibrillar strands, fewer on the fibrillar centers, and very sporadic on the granular aggregates. The same stainability of three nucleolar components with the Ag-AS method was also confirmed in the nucleoli segregated by actinomycin D. During the transition of growing oocytes from bilaminar to plurilaminar follicle stage, the nucleolar dense fibrillar strands gradually conglomerate and are transformed into large and compact spherules. The stainability of dense fibrillar components with the Ag-AS method was lost along with this nucleolar transformation. These results may provide some new clues on the functional significance of AgAS-positive proteins in the nucleoli.
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  • 21
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    Cell & tissue research 237 (1984), S. 103-109 
    ISSN: 1432-0878
    Keywords: Synapses ; Synaptogenesis ; Development fetal ; Olfactory cortex ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Electron microscopy was used to study synaptogenesis in prepyriform cortex of fetal rat pups during early stages of synapse formation. Of special interest is the frequent occurrence of unapposed, developing synaptic specializations in axon and growth cone profiles. The location and morphology of the unapposed specializations suggests that thay are presynaptic in nature. These presumably immature presynaptic specializations are found in the lateral olfactory tract and subjacent cortex. Intermediate forms between uncontacted presynaptic specializations and definitive synapses suggest a synaptogenic sequence in which initial development of an immature presynaptic specialization begins without apposition of a postsynaptic element at that location. This implies that initiation of presynaptic development is not dependent upon postsynaptic contact and also raises the question of whether synaptic contacts could be established via presynaptic induction of postsynaptic formation.
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  • 22
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    Cell & tissue research 237 (1984), S. 185-186 
    ISSN: 1432-0878
    Keywords: Gap junction ; Cytoskeleton ; Heart ; Ultrarapid freezing ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Using ultrarapid-freezing techniques and freezefracture electron microscopy, we report here a close association between cardiac gap junctions and specialized membrane domains containing regularly-spaced furrows. These specialized furrowed domains are observed only during periods of gap junction re-organisation (i.e., connexon redistribution) and may reflect the presence of underlying cytoskeletal elements controlling the position of connexons in the membrane.
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  • 23
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    Cell & tissue research 237 (1984), S. 371-373 
    ISSN: 1432-0878
    Keywords: Endothelium ; Transport, intracellular ; Transport vesicles, channels ; Micropinocytosis ; Capillaries ; Endometrium ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Three types of transendothelial channels are described in the endothelium of blood capillaries in the endometrium of the rat. It is postulated that they may function as pores draining interstitial fluid to the venous blood.
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  • 24
    ISSN: 1432-0878
    Keywords: Thyroid ; Immunocytochemistry ; Caldesmon ; Actin ; Endocytosis ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The distribution of caldesmon (a calmodulin-binding, F-actin interacting protein; Sobue et al. 1982) and actin was studied in the rat thyroid gland by means of light-microscopic immunocytochemistry, and the fine-structural distribution of actin filaments was examined by use of heavy meromyosin (HMM). Caldesmon and actin were demonstrated in the apical cytoplasm of almost all the follicle epithelial cells in normal as well as TSH-treated animals. Immunoreactivities for both caldesmon and actin showed almost the same pattern in localization. The smooth muscle cells of the blood vessels were also positive for caldesmon and actin. By electron microscopy, numerous actin filaments decorated by HMM and running perpendicularly or randomly to the apical surface were recognized in the apical cytoplasm of the follicle epithelial cell. These results suggest that caldesmon and actin, in conjugation with calmodulin, play a role in the regulation of cellular activity such as exocytosis and endocytosis in the apical portion of the follicle epithelial cell.
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  • 25
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    Cell & tissue research 235 (1984), S. 187-194 
    ISSN: 1432-0878
    Keywords: Golgi apparatus ; Monensin ; Small intestine ; Cytochemistry ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The effect of short-time treatment with the ionophore monensin, administered intraluminally at concentrations of 5 and 10 μM, was studied on the Golgi apparatus of absorptive cells in the small intestine of the rat. At 2–3 min after treatment most of the Golgi stacks exhibited dilated cisternae. At 4–5 min stacked cisternae were absent; they were replaced by groups of smooth-surfaced vacuoles. Dilatation and vacuolization occurred in the entire stacks without preferential effect on any particular Golgi subcompartment. Monensin did not influence the cytochemical Golgi reaction of thiamine pyrophosphatase and acid phosphatase. The characteristic staining pattern of these two enzymes in all Golgi cisternae of absorptive cells in the proximal small intestine, and the reactivity restricted to trans cisternae in distal segments of the small intestine, were unchanged after treatment with monensin. In the distal small intestine, the cytochemical pattern allowed the monensin-induced vacuoles to be attributed to the former cisor trans-Golgi face. Further, the cytochemical results demonstrate that vacuolization is not restricted to the stacked cisternae, but includes the trans-most cisterna. The latter, usually located at some distance from the Golgi stacks, has been defined as belonging to the GERL system in several types of cells. The clear response to monensin, an agent that selectively affects the Golgi apparatus, indicates common properties between trans-most and stacked Golgi cisternae.
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  • 26
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    Cell & tissue research 235 (1984), S. 485-489 
    ISSN: 1432-0878
    Keywords: Ventromedial nucleus ; Hypothalamus ; Ultra-structure ; Nucleoli ; Estrogen effects ; Chromatin ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Estrogen is accumulated from the blood by nerve cells in the ventromedial nucleus of the hypothalamus and can facilitate female reproductive behavior by acting on this region of the brain. This cell group was examined in ovariectomized female rats, given estrogen or control treatment, by use of light and electron microscopy. A significantly greater portion of the nerve cells in the estrogen-treated animals had protuberances on their nucleolar surfaces, apparent under the light microscope. The fine structure of such protuberances included dense, aggregated material, which is shown to contain DNA by the sodium tungstate staining technique. Because increased numbers of such protuberances were found in nuclei of cells of the experimental group where previous studies demonstrated a significant increase in ultrastructural signs of biosynthetic activity, they may be associated with increased RNA synthesis. Thus, they could indicate, ultrastructurally, increased synthetic rates for RNA in nerve cells through which estrogen promotes reproductive behavior.
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  • 27
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    Cell & tissue research 235 (1984), S. 669-673 
    ISSN: 1432-0878
    Keywords: Liver-cell heterogeneity ; Hepatic venous branches ; Karyometry ; Binucleate cells ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In untreated adult male albino rats nuclear volume and the percentage of binucleate cells were determined in the first layer of hepatocytes adjacent to hepatic venous branches of varying diameters (〈40 μm, 40 μm–80 μm, 80 μm–120μm, 120 μm–160 μm, 〉160 μm), and in the third and fourth layer of hepatocytes in the remainder of the perivenous parenchyma. In the first layer of hepatocytes adjacent to the vascular structures means of nuclear volume are significantly lower and percentage of binucleate cells significantly higher than in the cells of the remainder of the perivenous parenchyma. Within each area measured distribution curves of nuclear volume classes were homogeneous but showed heterogeneity in comparison with each other. The morphometric data presented in this study strongly support the opinion of the heterogeneity of liver cells in the perivenous zone, as previously postulated on the basis of histochemical investigations.
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  • 28
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    Cell & tissue research 236 (1984), S. 305-315 
    ISSN: 1432-0878
    Keywords: Hepatocytes ; Rat ; Liver ; Circadian rhythm ; Morphometry
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    Topics: Biology , Medicine
    Notes: Summary Subcellular structures of hepatocytes in periportal and perivenous zones were examined during 24 h. The volume, surface and numerical profile densities of cytoplasmic organelles were analysed morphometrically. Most subcellular structures in periportal and perivenous hepatocytes were subject to strong circadian variations. In hepatocytes from both zones, the volume densities of smooth endoplasmic reticulum (sER), mitochondria, lysosomes, peroxisomes, polysomes and lipid droplets demonstrated peak values at 16.00 h, 20.00 h or 00.00 h; trough values were at 04.00 h, 08.00 h, or 12.00 h, except for peroxisomes (16.00 h). However, the volume densities of glycogen granules and rough endoplasmic reticulum (rER) in periportal and perivenous hepatocytes exhibited maximal values at 04.00 h, 08.00 h or 12.00 h and minimal values at 20.00 h. The surface densities of sER, mitochondria, lysosomes and peroxisomes, and the numerical profile densities of mitochondria, lysosomes and peroxisomes in periportal and perivenous hepatocytes showed similar trends. These events suggest that membranes of the rER show a partial correlation with the sER, mitochondrial and lysosomal membranes during the 24-h span. This may involve the interaction between ribosomes and rER. Almost all cytoplasmic organelles examined displayed significant differences between periportal and perivenous hepatocytes, morphometrically and in fine structure, indicating that the morphofunctional variability of hepatocytes differs depending on the location in the liver acinus.
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  • 29
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    Cell & tissue research 236 (1984), S. 321-325 
    ISSN: 1432-0878
    Keywords: Triiodothyronine ; Radioautography ; Mitochondria ; Liver ; Rat
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    Topics: Biology , Medicine
    Notes: Summary To assess the distribution of the thyroid hormone triiodothyronine (T3) within intact living cells, freshly prepared dispersed rat hepatocytes were incubated with [125I]-T3 for periods of 5 min and 30 min. Lightand electron-microscopic (EM) radioautography was carried out to determine the distribution of grains over the isolated cells. Both procedures showed the grains distributed almost entirely over the cytoplasmic matrix rather than the nucleus. Grain counts under the EM were compared with expectation based on established quantitative methods. Only the mitochondria showed obvious and statistically significant grain counts, whereas the nucleus failed to accumulate grains in excess of expectations by chance alone based on area. The findings support the existence of mitochondrial binding of T3, presumably a prerequisite for its action in direct stimulation of the mitochondria.
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  • 30
    ISSN: 1432-0878
    Keywords: Lymph node ; Steroids ; Macrophages ; Intercellular junctions ; Rat
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    Notes: Summary Intercellular junctions were often found between macrophages in sinuses of regional lymph nodes of the rat after injection of large doses of cholesterol, cortisone acetate, and estrone at the footpad. They were identified by subplasmalemmal densities, 20–50 nm in width, beneath the plasma membranes of apposed macrophages. No distinct filamentous structures were visible in those dense regions. Electron-dense amorphous materials are lined up at the center of the intercellular space in the junctional regions. Some macrophages form clusters with intercellular junctions. No significant difference in the effect of cholesterol, cortisone acetate, and estrone on the number of intercellular junctions betwene macrophages was found.
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  • 31
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    Cell & tissue research 236 (1984), S. 171-180 
    ISSN: 1432-0878
    Keywords: Immunocytochemistry ; Ultrastructure ; Supraoptic nucleus ; Neuropil ; Rat
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    Topics: Biology , Medicine
    Notes: Summary The neuropil located ventral to the SON was investigated by the use of immunoperoxidase staining for neurophysins, oxytocin and vasopressin, and electron miroscopy. The study was performed in six groups of rats: 1) control; 2) infusion of isotonic saline into the CSF; 3) infusion of hypertonic saline into the CSF; 4) drinking hypertonic saline for 4 days; 5) same as group 4 but injection of colchicine into the CSF on second day of dehydration; 6) salt loading for 3 months. In the control rats the ventral neuropil contained a few immunoreactive processes, the general morphology of which was completely different from that of the neurosecretory axons emerging from the SON at its dorsal aspect. In rats of groups 3 to 6 the ventral processes (VP) became loaded with neurosecretory granules, whereas the perikarya and axons were depleted. Based on their general morphology and reactivity pattern it is suggested that the VP are dendrites. Most of these “dendrites” were embedded in a glial cushion formed by the processes of a particular type of marginal glia. Some of these “dendrites” enveloped an arteriole penetrating the optic tract. All VP were rich in synaptic contacts. The possibility that the VP of neurosecretory cells may be functionally related to the subarachnoid CSF and the arteriolar blood flow is discussed.
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  • 32
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    Cell & tissue research 236 (1984), S. 561-566 
    ISSN: 1432-0878
    Keywords: Pituitary gland, pars intermedia ; Peptide hormones ; Dopamine ; Corticotropin-releasing hormone ; 6-Hydroxydopamine ; Rat
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    Topics: Biology , Medicine
    Notes: Summary When rats were injected with 6-hydroxydopamine the catecholaminergic nerve terminals in their intermediate lobes exhibited distinct signs of degeneration. Morphometric examination of the Golgi apparatus in cells of the intermediate lobe of these rats showed significant enlargement of Golgi cisternae. The release of adrenocorticotropin, β-endorphin/lipotropin and α-melanotropin from intermediate-lobe cells in vitro was measured by radioimmunoassay. The high basal peptide release was inhibited by dopamine and stimulated by methyl-isobutyl-xanthine. In contrast, γ-aminobutyric acid, serotonin, histamine and noradrenaline, or corticotropin-releasing hormone, rat hypothalamic extract and vasopressin had no or only very weak effects. These observations indicate that the synthetic apparatus of intermediate-lobe cells is constantly depressed by dopaminergic nerves. We were not able to stimulate peptide release from intermediate-lobe cells by use of the abovementioned endogenous agents.
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  • 33
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    Cell & tissue research 237 (1984), S. 169-179 
    ISSN: 1432-0878
    Keywords: Foetal pancreas ; β Cells ; Insulin ; Fasting mothers ; Morphometry ; Rat
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    Topics: Biology , Medicine
    Notes: Summary After maternal fasting for 72 h the pancreatic β cells of 18-day-old foetal rats show a conspicuous enrichment in secretory material, with an increase of pancreatic insulin concentration and a marked development of the rough endoplasmic reticulum and the Golgi apparatus. The morphometric analysis shows that the intracytoplasmic migration of the secretory granules is inhibited, principally inside the cell web. Consequently the number of secretory granules fused with plasma membrane decreases and this is associated with a decreased foetal plasma insulin. The difference in the ultrastructural aspect of the β cells of foetuses from fasting mothers and of foetuses from fed mothers is less conspicuous at 19 days of gestation and progressively disappears at 20 and 21 days. The modifications in ultrastructural aspect and in functional state are discussed.
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  • 34
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    Cell & tissue research 238 (1984), S. 81-85 
    ISSN: 1432-0878
    Keywords: Retina ; Ibotenic acid ; Toxicity ; Rat
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    Topics: Biology , Medicine
    Notes: Summary After 2 h intraocular injections of 19 and 190 nmoles ibotenic acid in the rat retina produced an intensive vacuolization of the inner plexiform layer and cellular alterations, in the inner nuclear layer and ganglion cell layer. These alterations consisted of either cytoplasmic swelling accompanied by clumping of the nuclear chromatin or darkening of the cytoplasm along with nuclear condensation. A week later the retinas were thinner than the controls due to the disappearance of the affected cells. Pre-treatment with diazepam prevents the morphological alterations induced by 19 nmoles ibotenic acid; mainly the swelling, which was completely prevented, while the darkening was reduced drastically, although some vacuolization of the inner plexiform layer is still present.
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  • 35
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    Cell & tissue research 238 (1984), S. 159-163 
    ISSN: 1432-0878
    Keywords: Ovary ; Rat ; Cell division ; Luteinizing hormone
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    Notes: Summary The effect of an LH pulse on the rate at which 3H-thymidine is incorporated into cultured ovaries of metestrous rats was studied. In comparison to ovaries cultured with tonic LH, an LH pulse (1) “rescued” follicles from atresia, (2) induced thecal cell proliferation, and (3) increased the rate at which granulosa cells enter mitosis. It is concluded that LH pulses increase follicular growth by first triggering thecal cell proliferation and then inducing mitotic divisions within the granulosa cells of both atretic and non-atretic follicles.
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  • 36
    ISSN: 1432-0878
    Keywords: Choroid plexus ; Immunoglobulin G ; Permeability ; Anti-HRP-IgG ; Rat
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    Notes: Summary The localization of autologous antiperoxidase immunoglobulin G (IgG) was studied in the choroid plexus of Lewis rats immunized against horseradish peroxidase (HRP). This experiment was performed to study the permeability of the choroid plexus to intravascular IgG. It was shown that autologous IgG was present in the extravascular spaces. The transendothelial transfer appeared to occur mainly via the fenestrations and some interendothelial junctions. No transfer of IgG at the level of epithelial cells toward the cerebrospinal fluid was demonstrated. Interstitial spaces in contact with the connective-tissue cells of the choroid stroma were strongly labeled. The significance of these spaces remains hypothetical and raises the question of the fate of IgG from the interstitial space.
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  • 37
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    Cell & tissue research 238 (1984), S. 191-195 
    ISSN: 1432-0878
    Keywords: Glial fibrillary acidic protein (GFAP) ; Pituicytes, neonatal ; Development, ontogenetic ; Immunofluorescence ; Organ culture ; Rat
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    Notes: Summary The appearance and intracellular localisation of glial fibrillary acidic protein (GFAP) in pituicytes in neural lobe cultures of newborn rats aged 7 to 30 days were investigated by use of the indirect immunofluorescence method. GFAP-immunoreactive cells were observed mostly in the outgrowth zone. GFAP was localised in the perikaryal cytoplasm as well as in pituicyte processes. GFAP-positive pituicytes showed considerable morphological polymorphism. The presence of GFAP — astrocytic marker — in pituicytes in vitro and the evident morphological similarity to cultured astrocytes suggest the astroglial character of these cells.
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  • 38
    ISSN: 1432-0878
    Keywords: Cyclic guanosine monophosphate-phosphodiesterase (cGMP-PDEase) ; New cytochemical method ; Retina ; Rods, outer segments ; Light perception ; 5′GMPase ; Rat
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    Notes: Summary Cyclic guanosine monophosphate-phosphodiesterase (cGMP-PDEase) activity was studied histo- and cytochemically in the retinal rods of the rat with the use of a newly developed technique. Intense activity of cGMP-PDEase was evenly distributed over the outer segments of the rods. Reaction product was observed on the plasmalemma and on the disk membranes of the outer segments. A weak reaction product occurred also on the plasmalemma of the inner segments; however, no precipitate was found in the perinuclear and synaptic portions of the rod cells. The enzyme activity was strongly inhibited by 2 mM theophilline and by 2 mM 3-isobutyl-1-methylxanthine (IBMX). To confirm the specificity of this new cGMP-PDEase method, the localization of 5′nucleotidase (5′GMPase) was also studied. In contrast to the activity of cGMP-PDEase, the activity of 5′GMPase was distributed on the plasma membrane of the photoreceptor cells extending over a wide range from the synaptic endings in the outer plexiform layer to the tip of the outer segments.
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  • 39
    ISSN: 1432-0878
    Keywords: S-100 ; Müller cell ; Astrocyte ; Development ; Rat
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    Notes: Summary The rat retina was studied by immunohistochemistry with antibody to S-100 protein during the first three postnatal weeks. Immunoreactive astrocytes are first detected subjacent to the inner limiting membrane close to the optic disc. They gradually increase in number and spread toward the ora serrata along the inner surface of the retina as the development proceeds. S-100-immunostained Müller cells are first identified on the 12th postnatal day although their immunoreactivity is much weaker than that of astrocytes at the same stage. This differential intensity of the immunoreactivity of the two cell types facilitates observation of the entire shape of the astrocyte. This characteristic reveals that cellular investments of blood vessels in the inner retina are formed by astrocytic processes whereas those in the outer plexiform layer are derived from processes of Müller cells. The cellular investment becomes complete by the 18th postnatal day.
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  • 40
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    Cell & tissue research 238 (1984), S. 559-564 
    ISSN: 1432-0878
    Keywords: Adrenal cortex ; Macrophages ; Ovariectomy ; Rat ; Estradiol administration
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    Notes: Summary Macrophages of the adrenal cortex were studied in normal male and female, ovariectomized and estradiol-injected rats. In normal male rats few macrophages with numerous granules were observed in the zona fasciculatazona reticularis border, and in the zona reticularis. Granules, identified as lysosomes, were limited by a single membrane with a heterogeneous matrix; they exhibited acid phosphatase- and aminotriazole-resistant peroxidatic activities. A larger number of macrophages had identical distributions in normal female rats. In ovariectomized and estradiol-injected rats the number and distribution of adrenal macrophages were similar to those in normal females; however, in spayed animals the number of these cells in the zona reticularis was higher than in the other experimental groups. Lysosomes in macrophages of treated animals were more numerous and their contents more complex than in normal male animals. These results indicate that the adrenal macrophage system is stimulated in experimental conditions involving high levels of circulating estrogens.
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  • 41
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    Keywords: Sympathetic ganglion ; Development, ontogenetic ; Corticosteroid treatment ; Rat
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    Notes: Summary Hydrocortisone injections into rats on postnatal days 3–9 caused an increase in the number of small granulecontaining cells in the superior cervical ganglia. These cells, corresponding to the small, intensely fluorescent cells, showed an extensive rough endoplasmic reticulum, a large Golgi apparatus and a very large number of granular vesicles. In addition to the granular vesicles, 70–160 nm in diameter, in which the dense core filled most of the vesicle, most cells of the hydrocortisone-injected rats contained also larger granular vesicles, up to 350 nm in diameter, in which the dense core was eccentrically located. A minority of the cells contained only granular vesicles 70–100 nm in diameter, which was the only type seen in the saline-treated control rats. Thirty days after discontinuation of the hydrocortisone treatment, most of the cells with large granular vesicles had disappeared, and only two profiles of such cells were seen on day 40. The other small cells contained only granular vesicles 70–160 nm in diameter, and these cells could not be distinguished from the small granule-containing cells of 40-day-old control rats treated early postnatally with saline. Hydrocortisone treatment, first on days 3–9 and subsequently on days 40–46, caused reappearance of the small granule-containing cells with large granular vesicles up to 350 nm in diameter, the dense core of which was eccentrically located. Hydrocortisone treatment on days 40–46 only was not followed by appearance of such cells in rats treated with saline on days 3–9.
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  • 42
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    Cell & tissue research 238 (1984), S. 459-474 
    ISSN: 1432-0878
    Keywords: Meningeal compartment ; Perivascular space ; Brain intercellular compartment ; Cerebral cortex ; Rat
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    Notes: Summary The intercellular clefts of the brain and the leptomeninges, and the perivascular spaces were studied with reference to the results obtained in a previous study (Krisch et al. 1983). The spatial relationships of these compartments were analyzed at the electron-microscopic level. Horse-radish peroxidase (HRP) was injected into the brain or into the contralateral ventricle. The pattern of distribution of HRP depends on the boundary situation in the individual compartments. The inner and outer pial layers accompany the vessels intruding into the brain. In the Virchow-Robin space the pial funnel obliterates within a short distance. The inner arachnoid layer is continuous with the outer arachnoid layer when it covers the vessels traversing the meningeal space. The perivascular compartment is not in communication with the arachnoid space; moreover, the pial funnel within the Virchow-Robin space is sealed off against the arachnoid space. Thus, blood vessels traversing the meningeal spaces and subsequently penetrating the brain surface are exposed to the common intercellular compartment represented by the intercellular clefts of the brain and the leptomeninges; this compartment does not communicate with the other compartments. The cerebrospinal fluid located in this intercellular compartment is preferentially drained into the upper cervical lymph nodes.
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  • 43
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    Cell & tissue research 238 (1984), S. 635-642 
    ISSN: 1432-0878
    Keywords: Liver ; Endothelium ; Kupffer cells ; Peroxidase ; Cytochemistry ; Ultrastructure ; Rat
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    Topics: Biology , Medicine
    Notes: Summary Rat liver fixed by perfusion with low glutaraldehyde concentrations was incubated in diaminobenzidine-containing medium to stain for peroxidase. Endogenous peroxidatic activity was found not only in Kupffer cells but also in the endothelial cells lining the sinusoids and central veins. The reaction product was localized in the nuclear envelope and endoplasmic reticulum. The peroxidatic activity in endothelial cells showed a concentration-dependent sensitivity to glutaraldehyde: in liver samples fixed with 0.25% glutaraldehyde, approx. 23% of the sinusoidal endothelial cells and 65% of central vein endothelium were peroxidase-positive; with 0.5% glutaraldehyde, only approx. 8% of the sinusoidal endothelial cells contained detectable amounts of the reaction product; with 1.5% glutaraldehyde all endothelial cells were consistently peroxidase-negative. No peroxidatic activity could be found in liver endothelial cells following isolation by centrifugal elutriation. Endothelial cell peroxidase may possibly be involved in defense responses of liver and/or, as a part of prostaglandin synthase system, in prostanoid production.
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  • 44
    ISSN: 1573-5117
    Keywords: Sudan ; Schistosoma mansoni ; Biomphalaria pfeifferi ; miracidia ; sporocysts ; host-parasite relations ; laboratory studies ; decoy snails
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    Topics: Biology
    Notes: Abstract Laboratory experiments have been carried out to determine the susceptibility of Gezira Biomphalaria pfeifferi snails to S. mansoni miracidia and the relationship between miracidia and daughter sporocyst production at the 10–17 day development stage. The relationship between snail numbers, miracidia numbers and water volume has also been studied. Two non susceptible snails, Bulinus truncatus and Cleopatra bulimoides, both of which occur naturally in Gezira canals, were tested to see if they act as decoys for S. mansoni miracidia. The results showed that the B. pfeifferi are 100% susceptible to S. mansoni invasion, at least to the daughter sporocyst development stage. The more miracidia that penetrated the more daughter sporocysts were produced, however individual variation and overlap were great. When one miracidium was released to find one snail it succeeded in low water volumes (5 m, 50 ml), but failed in 5 litres. When 100 miracidia were released mortality of snails was high suggesting superinfection particularly when only one or five snails were available. Among survivors daughter sporocyst counts were very high. Cleopatra and Bulinus snails do have a decoy effect when present in large numbers. In their presence the number of infected snails was marginally reduced and the number of daughter sporocysts greatly reduced. However, if superinfection is reduced by decoy effect, it is conceivable that Biomphalaria may be protected by decoy snails in circumstances where miracidia counts are high.
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  • 45
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    Hydrobiologia 110 (1984), S. 241-245 
    ISSN: 1573-5117
    Keywords: Sudan ; pearl oyster ; feeding ; growth ; Red Sea ; Sudan
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    Topics: Biology
    Notes: Abstract Plankton samples and gut contents of P. margaritifera were analysed monthly from April 1972 to March 1973. Coscinodiscus sp. was the most ingested food by pearl oysters of all ages. However, food variety increased as the oyster grew older. Experiments confirmed the absence of food selectivity in P. margaritifera. Unlike the young ones, the adult showed reduced growth during summer (July–September), which coincides with its spawning season.
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  • 46
    ISSN: 1573-5117
    Keywords: Sudan ; Schistosoma mansoni ; Biomphalaria pfeifferi ; miracidia ; sporocysts ; transmission of schistosomiasis ; host-parasite relations ; ecology
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    Topics: Biology
    Notes: Abstract The efficiency of S. mansoni miracidia in locating and infecting Biomphalaria pfeifferi in Gezira canals has been studied under field conditions. When S. mansoni eggs were introduced into clean stagnant water in small field channels, the miracidia hatched to infect 100% of 30 snails in cages at the release point. Fifteen metres upstream and downstream 13% of caged snails were infected but no infections were found in snails 20 m away. When eggs were released into the same canal in flowing water (8.3 cm · s−1), no infections were detected in any of the caged snails placed 0–100 m downstream. Releasing hatched miracidia instead of eggs resulted in infections in all cages at 5 m intervals from 0-100 m. The release of eggs into flowing water was likened to the method by which S. haematobium eggs are deposited during urination. The 0% infection suggests that eggs will be swept away from the point of contamination by the flow. Thus only urination into stagnant water will lead to heavy snail infection rates. When eggs were released into a small pond-like minor canal tail end snail infection rates were only 3%. This was probably due to the larger water volume, smaller number of caged snails, and the presence of vegetation and other fauna which may be decoys or predators. The results highlight how very high snail infection rates can be produced under ideal conditions but also show how large snail and miracidia numbers are required in natural situations.
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  • 47
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    Hydrobiologia 110 (1984), S. 213-217 
    ISSN: 1573-5117
    Keywords: Sudan ; Penaeus ; food ; food selection ; chemoreception
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    Topics: Biology
    Notes: Abstract A study of food and food selection of Penaeus monodon (Fabricius) on the Sudanese Red Sea coast was carried out. Juveniles feed mainly on algal material. Adults feed on crustacea, annelids, algae, mud and unidentified matter. Adults were opportunistic in their feeding behaviour, but their preference was towards animal protein. Antennae, mouth parts and fore-chelipeds were found to carry long, slender setae which are probably of sensory function. Some setae on the carpus of the endopodite were serrated and seem to be used in grooming. Numerous tufts of setae in the chela of the fore periopods may hold a gustatory function. The inner edges of the chela are lined with a row of peg-like setae, which are probably another site of reception.
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  • 48
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    Hydrobiologia 110 (1984), S. 57-59 
    ISSN: 1573-5117
    Keywords: Sudan ; algae ; Blue Nile ; primary productivity ; tropical rivers ; White Nile
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Phytoplankton production and respiration in the Blue Nile and White Nile at Khartoum were measured during the period November 1969–January 1971 using the light and dark bottle technique. Maximum rates of production coincided with periods of maximum phytoplankton densities. In the Blue Nile gross production varied between 0.00 gCm−3d−1 during the flood season and 2.19 gCm−3d−1 (0.49 mgO2l−1h−1) during November 1969. In the White Nile the range was from 0.41 gCm−3d−1 (0.09 MgO2l−1h−1) in May to 3.74 gCm−3d−1 (0.83 MgO2l−1h−1) in November. The maximum rates of respiration in the Blue Nile and White Nile were 0.10 and 0.63 MgO2l−1h−1 respectively. The ratios net:gross production were generally higher in the White Nile than in the Blue Nile.
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  • 49
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    Hydrobiologia 110 (1984), S. 61-77 
    ISSN: 1573-5117
    Keywords: Sudan ; algae ; Nubian desert
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    Topics: Biology
    Notes: Abstract The algae contained in some plankton samples from several localities on four wadi in N.E. Sudan were examined. The observed algae amount to 260 taxa, of which the diatoms, representing 50% of the algal flora, are the most numerous. Other large groups are the blue-green algae (17%) and the green algae (24%) among which the desmids (6%) are rather unimportant.
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  • 50
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    Hydrobiologia 110 (1984), S. 109-111 
    ISSN: 1573-5117
    Keywords: Sudan ; mangroves ; Red Sea ; ecology
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    Topics: Biology
    Notes: Abstract An ecological survey of the mangrove vegetation in the littoral salt marshes of the Red Sea coast of the Sudan was made during short periods between 1980 and 1982. Avicennia marina (Forsk) Vierh occurs in pure stands of different dimensions in four types of locations. In each location, the responses of Avicennia are expressed in the degree of compactness of aggregation, canopy, height and regeneration potential. Grazing by camels is the primary agent in degradation of accessible locations; salt pans and to a limited extend cutting contribute in part to the process of degradation. The badly damaged aggregations may benefit from protection through control of grazing by nomadic camel tribesmen.
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  • 51
    ISSN: 1573-5117
    Keywords: Sudan ; Mollusca ; Gastropoda ; fresh water snails ; trematode parasites ; schistosomiasis
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    Topics: Biology
    Notes: Abstract An account is given of the aquatic gastropod molluscs obtained in a survey made to assess future ecological effects of the Jonglei Canal now under construction in the Sudd region of southern Sudan. A total of about 23 species of aquatic snail lives in this region, of which a number are utilised as intermediate hosts by trematode parasites infecting man, livestock and wild herbivores, causing the diseases schistosomiasis, fascioliasis and amphistomiasis. Public health and veterinary authorities should be alert to the possibility that the Jonglei Canal will increase snail populations and raise the prevalence of parasitic diseases.
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  • 52
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    Hydrobiologia 110 (1984), S. 273-279 
    ISSN: 1573-5117
    Keywords: Sudan ; Alestes ; growth ; feeding ; Nile
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    Topics: Biology
    Notes: Abstract The food, feeding habits and growth of 0+ Alestes nurse (Rüppel) from the Blue Nile near Khartoum, was investigated. 0+ A. nurse were observed to change their food from phytoplankton and small zooplankton during their pre-metamorphosis stages, to larger zooplankton (Daphnia spp., Chydorus sp.) and insects (larvae, pupae and imagines) during their post-metamorphosis stage. Their growth in length and weight was characterized by two distinct stanzas; during the first stanza, the fish increased in total length at a rate of 0.196 mm mm−1 wk−1, and in wet weight at a rate of 0.430 g g−1 wk−1. These rates were reduced during the second stanza to 0.046 mm mm−1 and 0.080 g g−1 wk−1, respectively. The calculated value of the exponent b in their length-weight relationship suggested that their growth was allometric.
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    Hydrobiologia 110 (1984), S. 281-286 
    ISSN: 1573-5117
    Keywords: Sudan ; frontal bone ; age determination ; cyprinidae ; Jebel Aulia
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    Topics: Biology
    Notes: Abstract In spite of the exuberance of recent approaches to age determination of fish, many ecologists are convinced that the most valid method is age reading from permanent marks or growth rings laid on skeletal parts. Of the known structures used, only the vertebrae are suitable and readable for a large variety of species and sizes. Examination of different skeletal structures of Labeo horie, a Cyprinid collected from Jebel Aulia reservoir, revealed that the frontal bone carried well-defined growth rings. The validity of these rings was checked against other direct and indirect methods of age determination. Results proved to be statistically valid, and the frontal bone offers an additional device of age determination of L. horie.
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  • 54
    ISSN: 1573-5117
    Keywords: Sudan ; abundance ; breeding ; sex-ration ; Tilapia ; Labeo ; White Nile
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    Topics: Biology
    Notes: Abstract Seasonal abundance, maturation and sex-structure were studied in populations of T. nilotica and L. niloticus in the Jebel Aulia reservoir and in the White Nile below the dam. L. niloticus showed a single annual breeding cycle from mid-July to December and was most abundant in October in the lake, and in early November in the river. T. nilotica showed two distinct annual seasons of abundance: summer (April to June) and autumn (November to October). Spawning activity was highest in summer (April to June) and autumn (late July to September). The sex-ratio varied inversely with age (size); males were predominant in populations of older fish, perhaps because of their greater endurance to adverse conditions as evidenced by their better survival in hypoxic water.
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    Hydrobiologia 110 (1984), S. 305-314 
    ISSN: 1573-5117
    Keywords: Sudan ; fish species ; selectivity ; abundance ; distribution ; fish production
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    Topics: Biology
    Notes: Abstract Developing lake conditions in Lake Nubia were found to offer good commercial fisheries potential. This prospect initiated fishery research since 1967. In this study, 26 fish species belonging to 10 families were recorded. Compared with previous studies the total number has decreased and some of the species that were commercially important during the early years of the lake formation, are now negligible in occurrence (Distichodus, Citharinus, Bagrus). The species widely distributed in the lake are Labeo niloticus, Lates niloticus, Sarotherodon niloticus, Hydrocynus forskallii, Alestes baremose and Eutropius niloticus. Their highest production occurs during two periods: February to May and July to September. The dominant fish species feed on different items in a balanced equilibrium with the ecological and biological conditions.
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    Hydrobiologia 110 (1984), S. 295-304 
    ISSN: 1573-5117
    Keywords: Sudan ; Clarias ; breeding ; maturation ; hormones ; growth ; cultivation
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    Topics: Biology
    Notes: Abstract The seasonal abundance, population structure, reproduction, feeding, respiratory adaptations and tolerance were studied in the catfish Clarias lazera (Cuv. & Val.) in the reservoir of Jebel Aulia on the White Nile, and in the Green-Belt canals near Khartoum. Continuous monitoring of the ripe, spent and spawning females and immature fish in the population revealed that C. lazera breed synchronously in the autumn in the reservoir and rather sporadically in the canals. Medium-sized females were more fecund than old and young females. Administration of pituitary extract enhanced maturation, induced spawning in mature fish, stimulated body growth, especially in maturing specimens, and produced, as a side-effect, a diuretic response. Mature fish depended on pulmonary respiration for about 45% of the total oxygen requirement. C. lazera fed preferentially on its fry and on decayed proteinaceous food. The suitability of this species for economic cultivation is discussed.
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    Hydrobiologia 110 (1984), S. 315-317 
    ISSN: 1573-5117
    Keywords: Sudan ; Lake Nubia ; fisheries
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    Topics: Biology
    Notes: Abstract The gill nets presently used in Lake Nubia fishing were subjected to a comparative study in regard to their relative efficiency. The results obtained indicate that Mesh 6 netted with Twine 4 is the most effective net, followed by Meshes 2, 4, 8 and 10 respectively. It has also been shown that, although individual nets undergo seasonal fluctuations in CUE, this does not, substantially affect their order of merit. The role of water characteristics on fish population distribution and subsequenct changes in the CUE was also noted.
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    Hydrobiologia 110 (1984), S. 319-325 
    ISSN: 1573-5117
    Keywords: Sudan ; Pisces ; Cyprinidae ; Barbus bynni ; breeding ; Jebel Aulia Reservoir
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Barbus bynni begins to mature at Age IV. Ripening of gonads of mature fish starts in May when water temperature approaches the annual maximum. However, the spawning season coincides with the onset of the flood season in July. These facts, as well as the cyclic growth of the gonads, show that B. bynni spawns once a year. Fecundity varies with size of fish and gonads. However, this levels off in the middle size group. At this age the fecundity was estimated to be 1 424 693 eggs.
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    Hydrobiologia 110 (1984), S. 327-332 
    ISSN: 1573-5117
    Keywords: Sudan ; Pisces ; Cyprinidae ; food and feeding
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    Topics: Biology
    Notes: Abstract Basic knowledge on the feeding ecology of one of the common and commercially important fish species in Jebel Aulia Reservoir is provided. The structure of the feeding apparatus indicates that Labeo niloticus is a bottom feeder, depending on soft and decayed vegetation, organic debris and whatever small organisms found within. However, juveniles and fry are prone to explore all layers and depths of the river selectively for plankton. There is little evidence of seasonal selection of food. Changes in diet quality are governed by the availability of type of food. Variability of feeding activity is connected with climate and breeding season.
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    Hydrobiologia 110 (1984), S. 333-338 
    ISSN: 1573-5117
    Keywords: Sudan ; fish ; Characidae ; growth of rings ; back-calculation ; aging structure
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    Topics: Biology
    Notes: Abstract The scales, opercula and vertebrae from 69 Hydrocyon forskali and 63 Alestes dentex were investigated for their reliability as aging structures. In both species the scales were unreliable due to their regenerative property, in spite of the clarity of their growth rings. Back-calculated lengths from the first growth ring laid on opercula and vertebrae resulted in values that coincided with the annual growth of the two species. In H. forskali, the mean back-calculated length from the two bones were 16.2 ± 5.9 and 16.8 ± 4.4 respectively, and for A. dentex, 11.1 ± 1.7 and 12.8 ± 1.8, respectively. Predictive equations for bone radius from fish length were written for both species. In this study, reliability was maximal between the observed and back-calculated lengths for both species (r 〉 0.95).
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    Hydrobiologia 110 (1984), S. 351-363 
    ISSN: 1573-5117
    Keywords: Sudan ; Polypterus ; aerial ; branchial ; respiration ; hypoxia ; hypercarbia
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    Topics: Biology
    Notes: Abstract The respiratory behaviour and partitioning of O2 uptake between air and water were investigated in Polypterus genegalus using continuous-flow and two-phase respirometers and lung gas replacement techniques P. senegalus rarely resorts to aerial respiration under normal conditions. Partitioning of O2 consumption depends on the activity and age of fish and the availability of aquatic oxygen. Immature fish (12–22 g) cannot utilize aerial O2 but older fish exhibit age-dependent reliance on aerial respiration in hypoxic and hypercarbic waters. Pulmonary respiration accounts for 50% of the total requirement at aquatic O2 concentrations of about 3.5 mg · l−1 (or CO2 of about 5%) and fish rely exclusively on aerial respiration at O2 concentrations of less than 2.5 mg · l−1. Branchial respiration is initially stimulated by hypercarbia (CO2: 0.5–0.8%) but increased hypercarbia (CO2 − 1%) greatly depresses (by over 90%) brancial respiration and initiates (CO2: 0.5%) and sustains pulmonary respiration.
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    Hydrobiologia 110 (1984), S. 339-349 
    ISSN: 1573-5117
    Keywords: Sudan ; Protopterus ; Polypterus ; Clarias ; ATP ; GTP ; development ; hypoxia
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    Topics: Biology
    Notes: Abstract The ATP and GTP contents of erythrocytes of Protopterus annectens, Polypterus senegalus and Clarias lazera were assessed in relation to maturation and hypoxia. The steady state ‘normal’ concentrations of ATP were: 446, 316 and 475 µM/100 ml and those of GTP were 286, 93 and 85 µM/100 ml in Protopterus, Polypterus and Clarias, respectively. The level of ATP decreased and that of GTP increased with increments in body growth, especially during development of the pulmonary organs. The concentration of GTP also increased progressively with hypoxia (O2: 7.4 to 3.6 mg/l); the level ATP remained almost unaltered. The ratio of GTP increased markedly with increased hypoxia particularly in the adaptive respiratory range of aquatic oxygen concentrations (6.5–4.8 mg/l) when the fish were forced to depart from branchial respiration and became reliant on air-breathing.
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    Hydrobiologia 110 (1984), S. 1-13 
    ISSN: 1573-5117
    Keywords: Sudan ; Nile ; hydrology ; control ; utilization
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    Topics: Biology
    Notes: Abstract The Nile is a vital resource of north-eastern Africa. After a description of its basin, and its hydrology,the different phases in the process of man gaining control on the river are discussed. Presently, the Nile is used for irrigation, the generation of hydroelectric power, navigation, and, of particular importance to limnologists, fisheries. Future developments in the management of the Nile are summarized.
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    Hydrobiologia 110 (1984), S. 15-19 
    ISSN: 1573-5117
    Keywords: Sudan ; RSC programme ; tailings ; impacts on environment
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    Topics: Biology
    Notes: Abstract The unique Red Sea environment has up to now been relatively unaffected by polluting consequences of Man's activities, except for evidence of oil along the coasts and reefs from transiting ships, harbours, industrial and human wastes with growing population and industrialization. The discovery, in the mid-sixties, of deposits of metalliferous muds, rich in heavy metals, and the probable mining and processing of these was expected to add to the risk of pollution. The Saudi-Sudanese Red Sea Commission, entrusted with the development and exploration of these non-living resources, has already set out an environmental study programme, more or less directly related to the conservation of the living marine resources. Within this framework, the studies have focused on the assessment and magnitude of possible risks for the environment resulting from a tailings disposal in the Atlantis 11 Deep over an extended period of time, and on the development of methods to minimize these risks. The results obtained so far, indicate that a well-controlled tailings disposal below 1 000 metre water depth would keep the environmental impact of such an operation in acceptable dimensions. But it is hoped that the forthcoming Pilot Mining Operations will be capable of clarifying some uncertainties through further computer modelling, monitoring a full scale tailings disposal test, using realistic input conditions and evaluation of the ecosystem.
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    Hydrobiologia 110 (1984), S. 33-44 
    ISSN: 1573-5117
    Keywords: Sudan ; lakes ; fauna ; distribution ; comparative survey
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    Topics: Biology
    Notes: Abstract Lakes Kundi and Keilak show large seasonal variations in area, but have permanent dry season residual areas rich in aquatic macrophytes. The faunas of the two lakes are similar, with L. Kundi (130 species recorded) being richer than L. Keilak (91 species). The fauna of L. Kundi includes the protomonad Rhipidodendron huxleyi, which has not been previously recorded in Africa. In L. Keilak we found Neozonocypris mirabilis, previously known only from L. Tanganika.
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    Hydrobiologia 110 (1984), S. 21-32 
    ISSN: 1573-5117
    Keywords: Sudan ; Blue Nile ; chemical composition ; tropical rivers ; White Nile
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    Topics: Biology
    Notes: Abstract Fortnightly measurements of physical and chemical variables were made at two locations on the Blue and White Niles near Khartoum from August 1968 to December 1970. Variables analysed from each river were: temperature, pH, total residue, current velocity, oxygen, alkalinity, phosphate, nitrate, ammonia, silica, sulphate, iron, calcium, magnesium, sodium, potassium and oxidizable organic matter. The seasonal variations of these factors in the two Niles are compared and the interrelationships existing between some of them are discussed. Comparisons with earlier studies on the Nile and with some tropical rivers are made. In the Blue Nile, the amounts of suspended matter and nutrients are largely dependent upon the flood regime. Nitrate, phosphate, iron, oxidizable organic matter and total residue increase considerably in the Blue Nile when the river is in flood (peaks: 1 880 µg NO3-N l−1; 0.31 mg Fe l−1; 3 842 mg total residue · l−1). In the White Nile, concentrations of nitrate, phosphate, iron, oxidizable organic matter and total residue attain their peaks during the rainy season (270 µg NO3-N l−1; 163 tag PO4-P l−1; 0.46 mg Fe · l−1; 502 mg total residue · l−1). In both rivers, alkalinity, calcium, sodium and potassium tend to increase during the dry season while declining in the rainy season. Silica is depleted at certain times of the year, yet relatively high concentrations are maintained throughout the year and were not expected to limit growth of diatoms. Fall in silica concentrations, unlike nitrate, phosphate and iron, was always followed by a rapid restoration of a high level. Silica and magnesium showed no response to changes in discharge rates.
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    Hydrobiologia 110 (1984), S. 47-55 
    ISSN: 1573-5117
    Keywords: Sudan ; algae ; Blue Nile ; plankton ecology ; tropical rivers ; White Nile
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    Topics: Biology
    Notes: Abstract The variation in numbers of the major planktonic algae in the surface waters of the Blue Nile and White Nile were followed for 29 months (August 1968–December 1970). In terms of biomass, the phytoplankton was dominated by diatoms and blue-green algae; other groups were of minor importance. High phytoplankton densities occurred during winter in both rivers, and during summer in the Blue Nile, with values in the latter river greater than in the former. Melosira granulata and its variety angustissima and Anabaena flos-aquae var. intermedia f. spiroides were the most important taxa in the two Niles. Melosira distans and Attheya zachariasi, which are reported for the first time in the present study, preponderated at certain times in the Blue Nile. Microcystisflos-aqua which had been a minor component of the phytoplankton in both rivers during the early 1950s, showed a profuse growth in the Blue Nile. Lyngbya limnetica, Anabaenopsis cunningtonii and A. tanganyikae, which constituted important components of the phytoplankton in the early 1950s, either disappeared or maintained themselves in reduced numbers during the present study. This change in the algal flora of the two Niles may be attributed to the construction of the Roseres dam across the Blue Nile in 1966 and to the invasion of the White Nile by Eichhornia crassipes in 1957.
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    Hydrobiologia 110 (1984), S. 79-90 
    ISSN: 1573-5117
    Keywords: Sudan ; swamp vegetation ; Sudd
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    Topics: Biology
    Notes: Abstract This paper describes vegetation in the nothern Sudd area of the Sudan. A visit by boat was made in April 1978 along the White Nile from Malakal to Lake No; to the inflow regions of the Bahr el Jebel, the Bahr el Zeraf and to the Bahr el Ghazal. Only the vegetation along the fringe of the rivers was observed as plants were tall and dense and it was not possible to penetrate into the inner swamps. The outer edge of the vegetation was fringed by extensive mats of free-floating Eichhornia crassipes which was easily dislodged and formed floating islands to be carried downstream. The main rivers, excluding the Bahr el Ghazal, were too deep and turbid to support beds of submerged species but plants such as Ceratophyllum demersum could be found amongst floating plants. Vossia cuspidata usually produced a broad zone to the landward of Eichhornia. It dominated the shallow water with a floating raft of stems growing out from the shore over the water surface. Massive stands of Cyperus papyrus and/or Phragmites karka, over 5 metres tall, dominated the vegetation behind and sometimes excluded the Vossia zone. The papyrus could form dense, floating rafts of rhizomes at the water's edge, but Phragmites always remained firmly rooted to the bottom. Floating papyrus and Vossia would become detached from the main beds and drifted downstream with Eichhornia. Creepers were common amongst papyrus but less common amongst Phragmites where the vegetation was generally more dense. Large stands of Typa domingensis were observed behind the fringe vegetation. The vegetation of the Bahr el Ghazal, in marked contrast to the rest, was stunted, and reasons for this are suggested. Species diversity was greater and the less turbid waters allowed considerable development of floating-leaved and submerged species. Estimates of fresh weights and heights of dominant vegetation types were attained but methods were very laborious and alternative ways have been suggested. The fresh weight of Eichhornia from a one metre square quadrat in the White Nile was around 70 kg. The shoots of Phragmites weighed 18.5 kg and Cyperus papyrus, 11.0 kg m−2.
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    Hydrobiologia 113 (1984), S. 93-98 
    ISSN: 1573-5117
    Keywords: tropical zooplankton ; swamps ; Sudan
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    Topics: Biology
    Notes: Abstract Samples from the main Nile channel yield a poor zooplankton, deficient in species and heavily laden with organic debris. Samples from side arms and floodplain lakes yield a richer zooplankton, often containing 15–20 species of rotifers and ten or more species of Crustacea. When the floodplain lakes are heavily vegetated the number of species increases. For the Crustacea the index of diversity (α) normally varies between 0.6 and 2.4, but in a side arm of the Bahr el Zeraf it reaches 6.1 and in Lake Ambadi on the Bahr el Ghazal it reaches 8.4. The diversity of the rotifers is somewhat higher, normally ranging between 2 and 4.5, but reaching 23 in Lake Ambadi. The zooplankton of Lake Ambadi is the most distinctive and contains several species not found at other stations. The reasons for the peculiar zooplankton of Lake Ambadi are discussed.
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    Journal of chemical ecology 10 (1984), S. 1007-1018 
    ISSN: 1573-1561
    Keywords: Rat ; Rattus norvegicus sp. ; odorants ; stress ; behavior ; open field ; corticosterone ; fox dropping ; ketone ; sulfur ; compounds ; tans ; mercaptoketones ; repellent ; structure-activity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The stress for 12 sulfur-containing synthetized volatiles was evaluated in male Wistar rats and compared to that for fox-dropping extract concentrate. Stress behavior was analyzed by quantifying various stress responses in a standard open field and measuring the increase in plasma corticosterone concentration. Nine compounds induced stress—a dihydrothiazole, two cyclic polysulfides, five mercaptoketones, and a mercaptan. For the mercaptoketones, the following structure-activity relationships were observed. Size can vary considerably; the mercapto group can be either alpha or beta and either secondary or tertiary. The keto group is not essential, since a structurally related mercaptan remains active. The mercapto group is essential for activity in mercaptoketones, since conversion to a methyl sulfide resulted in a neutral response. This type of odorant could function as an allomone and may have potential in rat control as an area repellent.
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    Hydrobiologia 110 (1984), S. 99-108 
    ISSN: 1573-5117
    Keywords: Sudan ; water hyacinth ; nutrients ; P, N, Fe ; growth ; White Nile
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    Topics: Biology
    Notes: Abstract The possibility that the stunted growth of the water hyacinth in Bahr el Ghazal river in Sudan is influenced by nutrient elements is considered. Greenhouse experiments were carried out to determine the effects of deficiency and mineral nutrient additions on the growth of this plant. The water hyacinth was found to grow at a wide range of nutrient levels. Maximum growth was recorded at 21 mg l−1 N, 62 mg l−1 P, and 0.60 mg l−1 Fe.
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    ISSN: 1573-5117
    Keywords: Sudan ; water hyacinth ; biological control ; weevils
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    Topics: Biology
    Notes: Abstract Water hyacinth Eichhornia crassipes (Mart.) Solms. is an aquatic weed that infests most of the White Nile system in the Sudan. Serious economical and ecological problems are caused by this weed. The two weevils Neochetina eichhorniae and Neochetina bruchi were imported and released in an attempted biological control against the weed. The adults of these weevils attack the plant and feed by removing tissues from the leaf pseudolamina and petioles. The larvae tunnel inside the petioles and the crown. The optimum temperature for feeding and development of both species is 25° C. Results obtained from stocking hyacinth plants with adults and larvae of both species separately revealed that N. bruchi is more efficient in checking the growth of the plant. The progeny of a pair of N. bruchi and N. eichhorniae reared separately on 41 hyacinth plants for a period of 61 days (one generation period) reduced their population growth by 25.4% and 12.7% respectively. The progeny of both species in a mixed culture reduced the growth of the plants by 22.5% in the same period, while in the control the population of the plants increased 136.6%.
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    Hydrobiologia 110 (1984), S. 113-130 
    ISSN: 1573-5117
    Keywords: Sudan ; rotifers ; taxonomy ; biogeography ; Africa
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    Topics: Biology
    Notes: Abstract A collection of rotifers contained in samples from the Blue, White and joint Niles in the Sudan, the Red Sea Hills, and Jebel Marra mountains is studied, and the previous literature on the rotifera of Sudan is reviewed. A total of 145 rotifer taxa are now known from this country. Ecological and distributional notes on selected species are added. Besides cosmopolitan, pantropical, and tropical species, a small but significant fraction of the fauna is of northern origin, possibly reflecting climatic conditions of the late-Pleistocene.
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    Hydrobiologia 110 (1984), S. 131-134 
    ISSN: 1573-5117
    Keywords: Sudan ; amphipoda ; Bogidiellidae ; systematic ; ground water fauna
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Description of Bogidiella nubica, from interstitial waters in the Sudan. It is the first Bogidiella species known for the African continent, except for the Mediterranean area.
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    Hydrobiologia 110 (1984), S. 135-136 
    ISSN: 1573-5117
    Keywords: Sudan ; Nile ; Acari ; Halacaridae
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    Topics: Biology
    Notes: Abstract Limnohalacarus africanus and L. fontinalis are recorded from Lake No. These are respectively the third and second records of these species, and represent considerable extensions of their known ranges.
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    Hydrobiologia 110 (1984), S. 163-169 
    ISSN: 1573-5117
    Keywords: Sudan ; Cladocera ; biogeography ; taxonomy ; Sudan ; Nile
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    Topics: Biology
    Notes: Abstract Twenty species of Cladocera are reported from the Nile, where lacustrine species dominate, and from Jebel Marra and the Red Sea Hills, where chydorids dominate. The community found in the Red Sea Hills is more typically desertic than that of Jebel Marra, which appears closely related to the fauna of the West and Central African Sahel.
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    Hydrobiologia 110 (1984), S. 171-175 
    ISSN: 1573-5117
    Keywords: Sudan ; Crustacea ; Bathynellacea ; Nile
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    Topics: Biology
    Notes: Abstract Nilobathynella predynastica n.g., n.sp. from Abri, Nubia, is the first representative of the Tribus Cteniobathynellini in the Nile Valley. Its relationship with the other Cteniobathynellini, as well as its biogeographical significance, are briefly discussed.
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  • 78
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    Keywords: Sudan ; freshwater ostracods ; Africa ; taxonomy ; zoogeography
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    Notes: Abstract A collection of freshwater ostracods from the Sudan is studied and 18 species are recorded. Paracypretta amati sp.n. is new to science. A redescription of the holotype female of Paracypretta aratra (Brady, 1904) (not recorded in this collection) and a provisional key to the species of Paracypretta are given. Heterocypris giesbrechtii (G. W. Müller, 1898) is re-described; Heterocypris sobrinus (Masi, 1932) is placed in the synonymy of H. fretensis; Hemicypris intermedia (Lindroth, 1953) is recorded for the first time since its original description and is redescribed, while Cyprinotus largereticulatus Rome, 1969 is transferred to Hemicypris. The zoogeographical composition of this fauna is briefly discussed.
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    Hydrobiologia 110 (1984), S. 177-180 
    ISSN: 1573-5117
    Keywords: Sudan ; Nubia ; groundwater ; Copepoda ; Nitocrella
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    Topics: Biology
    Notes: Abstract Description of Parapseudoleptomesochra attirei from a well in a dry wadi bed at At Tire, Nubia. Its closest relative is found in a semidesert area in Iran. The chorological implications of this finding are briefly discussed.
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    Hydrobiologia 110 (1984), S. 181-190 
    ISSN: 1573-5117
    Keywords: Sudan ; Odonata ; Nile ; Red Sea Hills ; biogeography ; deserts
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Twenty-one species of dragonflies are recorded from the Nile and the Red Sea Hills in the Sudan. The majority are species of africotropical origin, but wide-ranging and tolerant of strongly fluctuating environmental conditions. A small but significant fraction is restricted to the Nile Valley, and another fraction, found only in the Red Sea Hills, is composed of Eremian species of Palaearctic origin. Besides adults, the last-instar larvae of Pseudogrion niloticum and of Paragomphus pumilio are described and figured.
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  • 81
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    Hydrobiologia 110 (1984), S. 191-212 
    ISSN: 1573-5117
    Keywords: Sudan ; Copepoda ; Calanoida ; taxonomy ; biogeography ; Africa ; Nile
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract At least 11 or 12 calanoid taxa occur in the Nile system. One species is typical of the river and of Lake Chad (Th. galebi); two are restricted to Lake Victoria (Th. galeboides, T. stuhlmanni), but both might be only subspecies to more widespread species. One is restricted to Lakes Edward and George (T. worthingtoni) but is a little known species. No calanoids are on record from Lakes Albert and Kyoga, while the species reported from Lake Turkana (T. banforanus) is out of range, and almost certainly represents an erroneous record. Among the remaining species, four are East-African, ranging from the southern tip of the continent to the Ethiopian plateau and the Nile valley (P. schultzei, Th. mixtus, T. kraepelini, T. cf orientalis), while two are Sahelian species that span Africa from east to west (M. mauretanicus, T. processifer et ssp.). Two new synonyms are introduced. One new subspecies (T. processifier friedae) is described from the Ethiopian plateau. It is suggested that Tropodiaptomus orientalis (Brady, 1886), the type species of its genus, should not be considered a nomen dubium, but should be redescribed on its type female(s) and on topotypical males from Sri Lanka.
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  • 82
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    Hydrobiologia 110 (1984), S. 219-225 
    ISSN: 1573-5117
    Keywords: Sudan ; Bivalvia ; Corbicula ; ring formation ; White Nile
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The growth of C. consobrina of the White Nile near Khartoum was studied during March–December, 1977 from material collected from fish guts every two months. It was found that the growth season extends from February to July in correlation with the rise in temperature. Food was abundant and the concentration of the dissolved calcium in the river water was also increasing. During the flood season from July to October, the clams entered a resting state as a result of the annual siltation and consequently growth was arrested. Growth rings were found to be formed as a result of the siltation-induced resting state. There is evidence that little growth occurred during the cooler months from October to December. The development of the false rings on the shells is possibly a result of the changes in the water levels. Analysis of the growth of the adult clams showed that there is a clear size-time relation; there was close agreement between the calculated maximum sizes and the actual data collected from the field. Other examples from Sudanese inland waters were also discussed. The role of the annual hydrological cycles in regulating the populations activities and hence the growth of the individuals was found to be important.
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  • 83
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    Annals of biomedical engineering 12 (1984), S. 357-369 
    ISSN: 1573-9686
    Keywords: Oscillatory flow ; High frequency ventilation ; Unsteady pipe flow ; Turbulence ; Pulsatile flow ; Transition
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Technology
    Notes: Abstract Turbulence during pulsatile flow has been suggested as a possible mechanism to enchance the transport of gases during high-frequency ventilation. Experimental studies on oscillatory flow in straight, circular tubes have identified three types of flow: (a) laminar; (b) conditionally turbulent, in which high-frequency disturbances occur during the decelerating phase of the flow cycle but relaminarize by the beginning of the subsequent accelerating phase; and (c) fully turbulent flow, in which disturbances occur throughout the flow cycle. Fully turbulent flow has been observed only when a mean flow is present, and only laminar or conditionally turbulent flow has been observed for purely oscillatory flow. A critical Reynolds number based on the Stokes layer can be defined, and transition Reynolds numbers between 400 and 550 have been experimentally determined for purely oscillatory flow in a circular tube, although lower values are expected for physiological flows. There are some indications that the structure of oscillating turbulent flow is similar to steady turbulent flow, and preliminary work in our laboratory shows that the spectral content of flows during high-frequency ventilation is similar to that in steady turbulent flow.
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  • 84
    Publication Date: 2022-05-25
    Description: © 2008 The Author. This is an open-access article distributed under the terms of the Creative Commons Attribution Noncommercial License. The definitive version was published in Environmental Fluid Mechanics 8 (2008): 551-560, doi:10.1007/s10652-008-9076-5.
    Description: Experiments are reviewed in which a two-layer salt-stratified tank of water was mixed by turbulence. The density profile began as a single step and evolved to a smooth mixed profile. The turbulence was generated by many excursions of a horizontally moving vertical rod with Richardson number Ri 〉 0.9 and Reynolds Number Re 〉 600. There was almost perfect collapse of all the profiles to one universal profile as a function of a similarity variable. We develop a theoretical model for a simple mixing law with a buoyancy flux that is a function of internal Richardson number Rii. A similarity equation is found. A flux law that increases with small Rii and decreases with large Rii is considered next. Since no analytical solution is known, the similarity concept is tested by numerically integrating the equations in space and time. With buoyancy flux monotonically increasing with internal Richardson number, the similarity approach is valid for a profile starting from a slightly smoothed step. However, a shock forms for a mixing law with higher initial Rii (so that buoyancy flux decreases with Richardson number) and the similarity approach is invalid for those initial conditions.
    Keywords: Turbulence ; Mixing ; Stratified ; Similarity solution ; Layered fluid
    Repository Name: Woods Hole Open Access Server
    Type: Article
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  • 85
    Publication Date: 2022-05-25
    Description: © 2008 The Authors. This is an open-access article distributed under the terms of the Creative Commons Attribution Noncommercial License. The definitive version was published in Environmental Fluid Mechanics 8 (2008): 495-509, doi:10.1007/s10652-008-9107-2.
    Description: Estuarine turbulence is notable in that both the dissipation rate and the buoyancy frequency extend to much higher values than in other natural environments. The high dissipation rates lead to a distinct inertial subrange in the velocity and scalar spectra, which can be exploited for quantifying the turbulence quantities. However, high buoyancy frequencies lead to small Ozmidov scales, which require high sampling rates and small spatial aperture to resolve the turbulent fluxes. A set of observations in a highly stratified estuary demonstrate the effectiveness of a vessel-mounted turbulence array for resolving turbulent processes, and for relating the turbulence to the forcing by the Reynolds-averaged flow. The observations focus on the ebb, when most of the buoyancy flux occurs. Three stages of mixing are observed: (1) intermittent and localized but intense shear instability during the early ebb; (2) continuous and relatively homogeneous shear-induced mixing during the mid-ebb, and weakly stratified, boundary-layer mixing during the late ebb. The mixing efficiency as quantified by the flux Richardson number Rf was frequently observed to be higher than the canonical value of 0.15 from Osborn (J Phys Oceanogr 10:83–89, 1980). The high efficiency may be linked to the temporal–spatial evolution of shear instabilities.
    Description: The funding for this research was obtained from ONR Grant N00014-06-1-0292 and NSF Grant OCE-0729547.
    Keywords: Turbulence ; Estuaries ; Shear instability ; Buoyancy flux
    Repository Name: Woods Hole Open Access Server
    Type: Article
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