ALBERT

Description: Many of the difficulties of staining plastic embedded tissues for light and electron microscopy derive from physical exclusion of hydrophilic staining reagents by hydrophobic embedding media. Structures which stain most intensely with hydrophilic reagents usually contain less hydrophobic plastic than do non-staining structures. Such incomplete infiltration is apparently caused by exclusion of viscous, hydrophobic monomers by physically dense and/or well hydrated tissue elements. In keeping with this, generalized staining of tissues embedded in hydrophobic media does occur when hydrophobic reagents are used. Staining of plastic-free structures with single hydrophilic reagents or with sequences of such reagents, is, however, largely rate-controlled. The surprising similarity of hydrophilic and hydrophobic plastic embedding media is discussed. Limits of this simple model are explored, with a consideration of the roles of fixative and of monomer-tissue reactions

Description: The behaviour of Chelophyes has been analysed from the point of view of coordination between stem and nectophores, and an electron microscope study of the effectors and conducting elements has been carried out. Coordination between the stem and anterior nectophore involves two pathways, one epithelial and the other nervous. The nervous link consists of a bundle of small neurites and a single giant axon. There is some evidence that this mediates rapid escape behaviour. After the nerves have been cut, coordination is maintained via the epithelial route. Impulses can jump from epithelial cells into nerves but the transmission process is unclear. Neuro‐epithelial transmission involves conventional synapses. As in physonectid siphonophores, the stem has two nervous systems each with its own giant fibre, and a slow system, the endodermal epithelium. In the nectophore, marginal nerve centres generate a swimming rhythm. Conduction in the subumbrellar muscle is myoid. The exumbrellar epithelium and the subumbrellar endoderm are conducting tissues. Histological study reveals synapses in the predicted locations and gives details of myo‐epithelial organization and nervous layout. Novel histological features include elements resembling steroid‐secreting cells, which ensheath nerves and are innervated by them, and innervated giant non‐nervous cells lying between the nerve ring and the hydroecium. The subumbrellar muscle cells are shown to have sarcolemmal invaginations reminiscent of the f‐tubule system of vertebrate muscle.

Description: We studied the formation of exocellular precipitates of struvite (Mg NH4PO4.6H2O) by 96 kinds of calcite‐pro‐ducing bacterial strains isolated from soil. We also studied the influence of calcium ions on struvite precipitation. The number of strains producing struvite was 20. Only four consistently formed large amounts. These results seem to indicate that the bacterial precipitation of struvite is not a general phenomenon. The strains studied were taxonomically identified, and no relationship was found between the production of struvite and the taxonomic identity of such strains. Calcium, supplied as Ca acetate in the culture medium, appeared to inhibit the biological precipitation of struvite.

Description: There are many kinds of squid and cuttlefish in the oceans of the world, and the stock sizes of some are presumed to be large. However, few attempts have been made to quantitatively assess standing stocks. In Japan, the commercial yield ranges from 400,000 to 900,000 metric tons annually, accounting for about 50 to 80% of the total cephalopod catch in the world. Jigging surveys have been conducted since 1971 in the Sea of Japan and since 1973 in the Pacific Ocean off Japan. Their purpose has been to assess the abundance of oceanic squid. The stock size index (N) and the density index (F) for Todarodes pacificus, Ommastrephes bartrami, and Onychoteuthis borealijaponica were calculated for the Pacific from 1968 to 1979 and for the Sea of Japan from 1971 to 1979. N and F correspond reasonably well to periodic changes in the annual yield (Y) of T. pacificus in the Pacific and the Sea of Japan. Since Y is presumed to correspond well to abundance, the results of these surveys give rough estimates of T. pacificus abundance. Changes in N and F for O. bartrami and O. borealijaponica do not show good correspondence with Y, probably because the study area covers only a part of the range of the two species.

Description: Three large data sets on cephalopods are critically examined with respect to several sources of error: day-night avoidance, net size, mode of fishing, and patchiness. Catches are low and variable and results only suggest problems with present sampling techniques. Specific field sampling is suggested to quantify several of these sources of error. Volume filtered and time fished are compared as measures of "effort." Variability of volume filtered and net speed within a single tow are examined.

Description: This is a study of larval abundance and distributional pattern of the winter population of the Japanese common squid, Todarodes pacificus Steenstrup, based on plankton net collections made by the R/V Soyo-Maru during January to March (1959–1976) in the seas extending from the south of Kyushu to the Pacific coast of central and eastern Honshu. The larval abundance index (LI) was compared with CPUE (catch per boat day), an index of recruitment of adult stock size. The main spawning ground of this population is located to the southwest of Kyushu. The larvae (rhynchoteuthion) grow while they are advected by the Kuroshio Current to the northeast. Hence early larvae are more abundant in the west, while advanced larvae are more abundant in the east. Mortality seems to be highest for stages up to several millimeters DML than for later stages. The stock size of the Pacific winter population has fluctuated greatly. It declined since the peak 1960 year class to very low levels. The decrease of the stock size took place in three phases. 1960–1963: While the stock size (CPUE) of the 1960 year class was large, the larval population (LI) produced by this year class was very low, resulting in a decrease of recruitment (CPUE) in following years. 1964–1969: The larval abundance for the 1964 year class was second highest (next to 1960) but the recruitment was remarkably low, probably because of unfavorable conditions for survival during the period between larval and advanced stages. This failure caused a further decrease of larval abundance for the 1965 and subsequent year classes. 1970–1976: The larval abundance remains at a very low level and CPUE has declined since 1970. Because of the short life-span (one year) of this squid, abrupt decreases of larval abundance and/or recruitment have a serious effect on stock size. The future recovery of the stock will depend on biotic and physical conditions that are favorable for the survival of early stages south of Kyushu. A significant positive correlation between catch per effort of adults and the abundance of larvae (LI) in the following year indicates that larval abundance can be used to assess the size of the spawning stock of the winter population of T. pacificas.

Description: An examination of the knowledge about sampling Loligo opalescens populations leads to two general conclusions regarding the assessment of their abundance. First, it is suggested that studies concentrate on spawning ground organisms, since they aggregate during spawning, are commercially fished at this time, and their numbers can be assessed using a combination of data from market catch, adult and egg case densities, acoustic sensing, and perhaps larval densities. Second, it is suggested that large-scale acoustic surveys coupled with large midwater trawling activities be used to qualitatively assess adult organisms off the spawning grounds.