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  • Articles  (12)
  • Bone  (12)
  • climate change
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  • Springer  (12)
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  • 2000-2004
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  • 1983  (12)
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  • Articles  (12)
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  • Springer  (12)
  • American Geophysical Union
  • De Gruyter
  • Emerald
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  • 2000-2004
  • 1980-1984  (12)
  • 1970-1974
  • 1930-1934
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  • Physics  (11)
  • Technology  (1)
  • Education
  • Geosciences
  • Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
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  • 1
    Electronic Resource
    Electronic Resource
    Autoradiographic localization of target cells for 1α, 25-dihydroxyvitamin D3 in bones from fetal rats (1983)
    Springer
    Calcified tissue international 35 (1983), S. 177-182 
    Details
    ISSN: 1432-0827
    Keywords: Vitamin D ; Bone ; Osteoblasts ; Receptors ; 1,25-dihydroxyvitamin D3
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary Thaw-mount autoradiographic studies after injection of3H-1,25-D3 were conducted on 18-and 20-day-old rat fetuses. In maxillary bones, ribs, and tibia, nuclear concentration of radioactivity was found in osteoprogenitor cells and osteoblasts. Osteocytes and chondrocytes in epiphyseal plates were either unlabeled or weakly labeled. In competition experiments, nuclear concentration of radioactivity was blocked by the injection of a high dose of nonradioactive 1,25-D3 prior to the administration of the labeled hormone, but not by a similar dose of nonradioactive 25-D3. The results are interpreted as indicating that osteoprogenitor cells and osteoblasts are target cells for the direct action of 1,25-D3 on fetal bone.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02405028
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  • 2
    Electronic Resource
    Electronic Resource
    Studies on the role of vitamin D in early skeletal development, mineralization, and growth in rats (1983)
    Springer
    Calcified tissue international 35 (1983), S. 455-460 
    Details
    ISSN: 1432-0827
    Keywords: Vitamin D ; Vitamin D deficiency ; Bone ; Cartilage ; Bone development
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary The role of vitamin D in early skeletal development was studied by measuring serum calcium and phosphorus, osseous tissue quantity and mineralization, and endochondral bone elongation in rat fetuses and pups from vitamin D-replete and vitamin D-deficient mothers. At the 20th day of pregnancy there was a slight, yet significant, increase in the amount of osteoid on trabecular bone surfaces in fetuses from vitamin D-deficient mothers. The fetal bones otherwise appeared normal in spite of severe skeletal changes in the vitamin D-deficient mothers. After parturition, the importance of vitamin D in skeletal development becomes progressively more obvious. Serum calcium levels were slightly, yet significantly, lower in vitamin D-deficient than in vitamin D-replete pups and these levels continued to fall in the vitamin D-deficient pups through lactation and after weaning. At 3 days postpartum, there was a small, yet significant, increase in the amount of osteoid on bone surfaces of the vitamin D-deficient pups. The relative amounts of osteoid in the vitamin D-deficient pups continued to increase through lactation and after weaning when compared with vitamin D-replete pups. By the 14th day of lactation and at later periods, there were significant reductions in metaphyseal mineralized tissues in the vitamin D-deficient pups when compared with the vitamin D-replete pups. At weaning and after weaning, there were substantial increases in growth plate thickness and decreases in longitudinal bone growth in the vitamin D-deficient pups when compared with the vitamin D-replete pups. The results from this study indicate that vitamin D does not appear to play a major role in fetal skeletal development. However, after birth, vitamin D becomes progressively more important with age for normal bone development, mineralization, and endochondral growth.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02405076
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  • 3
    Electronic Resource
    Electronic Resource
    Identification of a bone matrix-derived chemotactic factor (1983)
    Springer
    Calcified tissue international 35 (1983), S. 481-485 
    Details
    ISSN: 1432-0827
    Keywords: Chemotaxis ; Bone ; Osteoblasts ; Bone proteins
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary When demineralized bone matrix powder is implanted subcutaneously in the rat, the early responses involve the appearance and proliferation of mesenchymal cells at the site of implantation, followed by cartilage and bone formation. The ability of cells to migrate to the implant suggests that chemotaxis may be a critical event in this process. Therefore, using the modified Boyden chamber assay, we tested extracts of demineralized bone matrix for chemotactic activity. We have identified and partially purified, on molecular sieve chromatography, a heat labile and trypsin-sensitive protein (Mr=60,000–70,000) that is a potent chemoattractant for mouse calvaria, osteoblast-like cells (MMB-1), but not for monocytes (putative osteoclast precursors). These findings suggest that chemotactic protein(s) have a significant role in the recruitment of osteoprogenitor cells to a site of bone repair.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02405081
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  • 4
    Electronic Resource
    Electronic Resource
    Cyclical uptake pattern of tetracycline in post-secretory maturation phase enamel demonstrated in rooted teeth (1983)
    Springer
    Calcified tissue international 35 (1983), S. 762-766 
    Details
    ISSN: 1432-0827
    Keywords: Enamel ; Maturation cycles ; Tetracycline ; Dentine ; Bone
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary Uptake of tetracycline by enamel in the short-term was studied at an advanced stage of crown formation and after completion of crown formation in deciduous molars in the cat. Both secretory phase enamel and bands of postsecretory, maturation phase enamel labeled rapidly. The pattern of labeling mimicked that seen in the continuously growing, rootless incisor teeth of the rat, with narrow doublets fusing to form narrow bands with wide unlabeled intervals in the short term. This is a physiological demonstration which indicates that cyclical activity and changes may occurin vivo during the maturation phase of amelogenesis in rooted teeth. It is also noted that dentine did not, and that some circumscribed patches of bone did label in the same animals in the same time interval. Short-term tetracycline labels are lost following conventional histological processing, but are retained after freeze-drying or air-drying.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02405120
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  • 5
    Electronic Resource
    Electronic Resource
    Errors in measuring trabecular bone by computed tomography due to marrow and bone composition (1983)
    Springer
    Calcified tissue international 35 (1983), S. 148-152 
    Details
    ISSN: 1432-0827
    Keywords: Bone ; Computed tomography ; Densitometry ; Osteoporosis ; Osteomalacia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary The linear attenuation coefficient (μ in cm1) of trabecular bone was modeled for different conditions of bone and marrow composition in order to assess their influence on computed tomography (CT) quantitation. A large relative change (10% of TBV at 15% TBV) of bone concentration resulted in small changes of μ: 2.3% at 60 keV, 3.4% at 44 keV, 5.2% at 29 keV. Relative changes of trabecular bone volume (TBV) on the order of 3% could be detectedin vivo by CT were it not for errors of relocation and for compositional influences on accuracy. The μ (and density) depended critically not only on amounts of bone substance and marrow but on their compositions. Normal variation in the composition of bone substance produced an uncertainty in μ equivalent to 0.5 to 1% TBV. Increases of yellow marrow produced a decrease of μ which could be mistaken for a decrease of bone concentration. The biological variation (90% confidence limit) of marrow composition gives an uncertainty at 15% TBV of about 2.4% TBV at 60 keV, 1.7% at 44 keV, and 1.3% at 29 keV. These correspond to relative uncertainities of 16, 11, and 9% respectively. These factors help explain the large accuracy errors (30%) observed in all studies of trabecular bone where single-energy CT was used. Marrow composition also can affect precision of bone measurement. Systematic shifts of red and yellow marrow could mask biological changes such as those occurring with aging or treatment.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02405022
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  • 6
    Electronic Resource
    Electronic Resource
    Regional measurement of bone calcium accretion rate and exchangeable pool with a whole-body counter: Method and studies in subjects without bone disease (1983)
    Springer
    Calcified tissue international 35 (1983), S. 21-28 
    Details
    ISSN: 1432-0827
    Keywords: Bone ; Calcium metabolism ; Whole-body counting
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary A whole-body counter was used to measure the retention and distribution of radioactivity along the longitudinal axis of the body during 10 days after the intravenous injection of 50 µCi of47Ca. These data and the simultaneous measurements of the serum specific activity allowed us to calculate, by the Bauer-Carlsson-Lindquist (BCL) formulas generalized by Marshall, the calcium accretion rate and exchangeable pool in 7 areas: skull, thorax, pelvis, thighs, knees, legs, and ankles and feet. For the whole body, the accretion rate was 336±115 mg/24 h, and the exchangeable pool 5668±1221 mg, in 26 subjects without bone disease. Both parameters were significantly correlated with body height; the exchangeable pool was significantly higher in men than in women. The accretion rate and exchangeable pool expressed on a basis of bone content varied widely from one area to another, reflecting the ratio of spongy to compact bone: the thorax, pelvis, and arms, which contain 49% of the skeletal mass, accounted for 57% of the total accretion rate and 53% of the exchangeable pool; the skull, 18% of the skeletal mass, accounted for 14% of the accretion and 11% of the pool.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02405001
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  • 7
    Electronic Resource
    Electronic Resource
    Parathyroid hormone stimulates dentin and bone apposition in the thyroparathyroidectomized rat in a dose-dependent fashion (1983)
    Springer
    Calcified tissue international 35 (1983), S. 586-590 
    Details
    ISSN: 1432-0827
    Keywords: Dentin ; Bone ; Apposition rate ; Parathyroid hormone
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary The dose-dependency of the effects of parathyroid hormone (PTH) on bone and dentin apposition after both intermittent and continuous administration of the hormone was investigated. The purpose was to compare the sensitivity of these two mineralizing tissues to parathyroid hormone and to provide additional information regarding the direct effect of PTH on mineralized tissue formation. Adult rats were thyroparathyroidectomized and 5 groups of 4 or 5 rats each were given daily subcutaneous injections with different doses of bovine parathyroid hormone. Five more groups of 4 rats each were administered equivalent dosages by means of a continuous infusion pump implanted subcutaneously. An additional group of 4 rats served as controls. All animals were labeled with tetracycline injected on days 7, 9, and 11. The animals were killed on day 12 and blood samples were collected for serum calcium determination. The lower metaphysis of the femur and the left and right mandibles were dissected out, and undecalcified sections of plastic-embedded tissues were prepared. The distances between the three tetracycline bands were measured to determine the amount of bone or dentin formation. Results indicated that both dentin and bone apposition increased with higher dosages of hormone. No overall effect of the method of administration was evident. For both methods, bone apposition showed a more pronounced increase over the control levels than dentin apposition. This suggests that, although both osteoblasts and odontoblasts appear to respond directly to PTH, differences do exist in the magnitude and dose-dependency of the response. No causal relationship was found between increases in serum calcium levels and either bone or dentin apposition at the lower dose levels.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02405098
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  • 8
    Electronic Resource
    Electronic Resource
    A correlation analysis between bone formation rate and bioelectric potentials in rabbit tibia (1983)
    Springer
    Calcified tissue international 35 (1983), S. 728-731 
    Details
    ISSN: 1432-0827
    Keywords: Bone ; Bioelectricity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary The origin of the bioelectric potentials in unstressed living bones is still an open question. Blood-flow in vessels, stationary potentials on peripheral nerves, muscle injury potentials, and viability of bone cells are claimed to be possible sites of origin of the electric potentials recorded on bone surface. The present data show that the topographic quantitative distribution of tetracycline labeling at sequential levels of rabbit tibia and the distribution pattern of the bioelectric potentials in this bone are significantly superimposable. This coincidence of bone formation rates and bioelectric potentials seems to support the view that the latter are in some way linked with the laying down of new bone.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02405114
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  • 9
    Electronic Resource
    Electronic Resource
    Neutral peptidase activities in matrix vesicles from bovine fetal alveolar bone and dog osteosarcoma (1983)
    Springer
    Calcified tissue international 35 (1983), S. 791-797 
    Details
    ISSN: 1432-0827
    Keywords: Bone ; Osteosarcoma ; Matrix vesicles ; Alkaline phosphatase ; Aminopeptidase ; Naphthylamidase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary Extracellular matrix vesicles from bovine fetal alveolar bone and from a dog osteosarcoma were isolated by differential centrifugation and then fractionated on a discontinuous sucrose density gradient. The fractions were examined by electron microscopy and were analyzed for protein, alkaline phosphatase, aminotripeptidase, and four different β-naphthylamidase activities. The low-density peak of enzyme activities was shown by electron microscopy to be much more homogeneous than the crude matrix vesicle fraction. Two major peaks of protein and enzyme activities were present, one in the high and one in the low density layers. There was good correlation between the activities of alkaline phosphatase and the various peptidases in the fractions from the sucrose density gradient. These results indicate a coexistence of peptidase and alkaline phosphatase in matrix vesicles. On the other hand, there was generally no correlation between the peptidase and alkaline phosphatase activities in vesicular specimens from bovine liver obtained in the same way. Most of the peptidase activity and about half of the alkaline phosphatase activity were solubilized from bone matrix vesicles by detergents. The extracted alkaline phosphatase and alanyl β-naphthylamidase activities were separated from each other on a DEAE-cellulose column.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02405125
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  • 10
    Electronic Resource
    Electronic Resource
    X-ray diffraction studies of the crystallinity of bone mineral in newly synthesized and density fractionated bone (1983)
    Springer
    Calcified tissue international 35 (1983), S. 202-209 
    Details
    ISSN: 1432-0827
    Keywords: Bone ; Mineral ; Crystallinity ; Maturation ; Age
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary The crystallinity of bone mineral at different stages of maturation has been measured by quantitative X-ray diffraction methods. Crystallinity measurements were made on tibial middiaphyses from 17-day embryonic chicks, newlyformed periosteal bone from embryonic chicks, and density-fractionated bone from post-hatch chickens from 5 weeks to 2 years of age. For a given animal age and degree of mineralization, crystallinity increases with animal age, indicating that changes in bone mineral occur even after mineralization is complete or nearly complete.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02405032
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