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  • Immunohistochemistry
  • Springer  (20)
  • American Society of Hematology
  • Annual Reviews
  • Copernicus
  • Institute of Physics
  • Nature Publishing Group
  • 2005-2009
  • 1990-1994
  • 1980-1984  (20)
  • 1960-1964
  • 1983  (20)
Collection
Keywords
Publisher
  • Springer  (20)
  • American Society of Hematology
  • Annual Reviews
  • Copernicus
  • Institute of Physics
    • +
Years
  • 2005-2009
  • 1990-1994
  • 1980-1984  (20)
  • 1960-1964
Year
  • 1
    Electronic Resource
    Electronic Resource
    Immunohistochemical localization of types I, II, and III collagens in the ossified posterior longitudinal ligament of the human cervical spine (1983)
    Springer
    Calcified tissue international 35 (1983), S. 159-163 
    Details
    ISSN: 1432-0827
    Keywords: Anticollagen antibodies ; Collagen types ; Immunohistochemistry ; Ossified posterior longitudinal ligament
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary Immunohistochemical localization of types I, II, and III collagen in the ossified posterior longitudinal ligament of the cervical spine was studied using type-specific anticollagen antibodies. In contrast to the normal ligament which contains both types I and III collagens, the ossified matrix, composed of lamellar bone, contains only type I collagen, except for Haversian canals where type III is located in the inner wall. In the transitional region of preossifying ligaments, types III and I are both present. Type II collagen is present in the hyperplastic matrix of the ligament, and cartilage-like cells surrounded by type II collagen are aligned along nonossified ligaments adjacent to the preossifying region. A possible mechanism of matrix transition during the ossification process is given attention.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02405024
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  • 2
    Electronic Resource
    Electronic Resource
    Distribution of monoamine-containing nerve fibers in the pineal organ of untreated and sympathectomized dogs (1983)
    Springer
    Cell & tissue research 234 (1983), S. 519-531 
    Details
    ISSN: 1432-0878
    Keywords: Pineal organ ; Serotonin ; Immunohistochemistry ; Fluorescence histochemistry ; Sympathectomy ; Dog
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Monoamine (noradrenaline and serotonin)-containing nerve fibers distributed in the pineal organ of the dog were studied by means of histochemistry (formaldehyde-induced fluorescence) and immunohistochemistry (peroxidase-antiperoxidase-PAP method) with the use of a serotonin antiserum. With the fluorescence-histochemical technique a dense network of blue-green fluorescent fibers was demonstrated in the pineal organ. Most of these fibers formed a perivascular plexus and their branches penetrated into the intercellular spaces of the parenchymal cells. Since these fibers completely disappeared ten days after bilateral removal of the superior cervical ganglia, it was confirmed that they are noradrenergic post-ganglionic sympathetic nerve fibers. A few yellow-fluorescent fibers were detected in the proximal part of the organ after ganglionectomy. By the use of the PAP method, intensively immunoreactive parenchymal cells and nerve fibers were demonstrated. The distribution pattern of these fibers was similar to that of the fluorescent sympathetic fibers. After almost all immunopositive fibers had been abolished by sympathectomy, some serotonin-containing fibers remained. The latter could be traced back to a system of serotonin fibers in the epithalamic region. These findings suggest that 1) the sympathetic noradrenergic fibers in the pineal organ of the dog take up serotonin which is released from the pinealocytes, and 2) this organ receives a dual monoamine innervation via peripheral noradrenergic and central serotonergic nerve fibers.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00218648
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  • 3
    Electronic Resource
    Electronic Resource
    CRF-immunoreactive nerve fibers in the circumventricular organs of the monkey, Macaca fuscata (1983)
    Springer
    Cell & tissue research 232 (1983), S. 679-683 
    Details
    ISSN: 1432-0878
    Keywords: CRF-immunoreactive nerve fibers ; Circumventricular organs ; Immunohistochemistry ; Monkey, Macaca fuscata
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The occurrence of CRF (corticotropin-releasing factor)-immunoreactive nerve fibers in the circumventricular organs of adult male monkeys, Macaca fuscata, was studied on serially sectioned brains, by means of the peroxidase-antiperoxidase technique in combination with a highly specific and sensitive CRF antiserum. CRF-containing nerve fibers were found in high concentrations in the infundibulum and, in addition, in small numbers in the posterior lobe, organum vasculosum laminae terminalis, subfornical organ, and area postrema; they were missing in the pineal body and the subcommissural organ. The CRF immunoreactive nerve fibers distributed in these organs were located in the proximity of the blood vessels.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00216438
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  • 4
    Electronic Resource
    Electronic Resource
    Distribution of immunoreactive thyrotropin-releasing hormone in the forebrain and hypophysis of the bullfrog, Rana catesbeiana (1983)
    Springer
    Cell & tissue research 233 (1983), S. 507-516 
    Details
    ISSN: 1432-0878
    Keywords: Thyrotropin releasing hormone ; Rana catesbeiana ; Hypothalamus ; Immunohistochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The distribution of immunoreactive thyrotropin-releasing hormone (TRH) in the forebrain and hypophysis of Rana catesbeiana was studied by means of specific radioimmunoassay and immunohistochemistry based on peroxidase-antiperoxidase (PAP) techniques. A relatively high concentration of immunoassayable TRH is present in the hypothalamus. Immunoreactive TRH cell bodies are found in the anterior part of the preoptic nucleus, the dorsal infundibular nucleus, the nucleus of diagonal band of Broca, and the medial part of the amygdala. Immunoreactive nerve terminals are observed in the neurohypophysis and the external layer of the median eminence, where the terminals are in close contact with the capillary loops of the hypophyseal portal vessels. The possible role of TRH in the frog brain is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00212220
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  • 5
    Electronic Resource
    Electronic Resource
    Immunofluorescent localization of cGMP, cGMP-dependent protein kinase, calmodulin and cAMP in the rat uterus (1983)
    Springer
    Cell & tissue research 233 (1983), S. 639-646 
    Details
    ISSN: 1432-0878
    Keywords: cGMP ; Calmodulin ; Immunohistochemistry ; Estrogen ; Cell growth regulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Cyclic guanosine 3′, 5′ monophosphate (cGMP), cGMP-dependent protein kinase, calmodulin and cyclic adenosine 3′, 5′ monophosphate (cAMP) were localized in the uterus of the immature rat by an indirect immunofluorescence technique. cGMP, cGMP-dependent protein kinase and calmodulin were detected predominantly along epithelial and myometrial plasma membranes and in the adjacent cytoplasm. In contrast, cAMP immunoreactive material was found principally in the cytoplasm of connective tissue. After administration of 17 β estradiol, similar time-dependent changes were observed in the localization of cGMP, cGMP-dependent protein kinase and calmodulin in all uterine cell types. For the three compounds, nucleolar-like distribution of the immunofluorescence appeared approximately 12 h after treatment. A more dispersed, reticular distribution of the nuclear fluorescent staining was observed 20–24 h after hormonal treatment. Estrogen did not affect the localization of cAMP. The simultaneous mobilization of cGMP, cGMP-dependent protein kinase and calmodulin towards the same nuclear loci suggests concerted roles for these three molecules in nuclear metabolic processes during the development of the uterotrophic action of estrogens.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00212231
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  • 6
    Electronic Resource
    Electronic Resource
    An immunohistochemical survey of endocrine cells and nerves in the proximal small intestine of the platypus, Ornithorhynchus anatinus (1983)
    Springer
    Cell & tissue research 234 (1983), S. 153-164 
    Details
    ISSN: 1432-0878
    Keywords: Immunohistochemistry ; Gastrointestinal endocrine cells ; Brain-gut peptides ; Small intestine ; Platypus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The relative frequencies of endocrine cells and peptidergic nerve elements in the proximal small intestine of the adult platypus were studied by immunohistochemistry. Six kinds of endocrine cells — serotonin (5-HT)-, somatostatin-, gastrin-, motilin-, cholecystokinin (CCK) and bovine pancreatic polypeptide (BPP)-immunoreactive cells — were identified in this study. These endocrine cells were found most frequently in the intestinal glands, in moderate numbers in the tubular ducts and were infrequent in the surface folds. 5-HT-immunoreactive cells were most numerous, somatostatin-, gastrin-, motilin and BPP-immunoreactive cells were moderately numerous, whereas CCK-immunoreactive cells were rare. Five kinds of neuropeptides: substance P, vasoactive intestinal polypeptide (VIP), gastrin releasing peptide (GRP), somatostatin and leuenkephalin, were detected in the intramural nerve elements. Substance P-, VIP and GRP-immunoreactive nerve fibers were found most frequently in the lamina propria mucosae of the surface folds. The relationships between the possible functions of the peptides and amine detected in this study as well as the characteristic structure of the digestive tract of the adult platypus are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00217409
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  • 7
    Electronic Resource
    Electronic Resource
    Bombesin-, gastrin/CCK-, 5-hydroxytryptamine-, neurotensin-, somatostatin-, and VIP-like immunoreactivity and catecholamine fluorescence in the gut of the elasmobranch, Squalus acanthias (1983)
    Springer
    Cell & tissue research 234 (1983), S. 595-618 
    Details
    ISSN: 1432-0878
    Keywords: Neuropeptides ; Gut hormones ; Enteric nervous system ; Immunohistochemistry ; Elasmobranchs
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The presence of peptides and 5-hydroxytryptamine (5-HT) in neurons and endocrine cells in the gastrointestinal tract of the spiny dogfish, Squalus acanthias, was investigated by means of immunohisto-chemistry, and the distribution of catecholamines by use of the Falck-Hillarp fluorescence-histochemical technique. Bombesin-like immunore-activity was present in numerous nerves in all layers and all parts of the gut, and also in endocrine cells in the mucosa throughout the stomach, rectum and intestine. VIP-like immunoreactivity occurred in an abundance of nerve fibres and in nerve cell bodies in all parts of the gut except the oesophagus, while 5-HT-like immunoreactivity was found sparsely in nerve fibres and more frequently in endocrine cells throughout the gut. Gastrin/CCK-like immunoreactivity was present in numerous nerve fibres in the rectum, but only in scattered fibres in the other parts of the gut. Endocrine cells showing gastrin/CCK-like immunoreactivity were present in the intestine only. Somatostatin-like immunoreactivity occurred in both nerve fibres and endocrine cells of the stomach and intestine, but only in nerves in the rectum. Neurotensin-like immunoreactivity was confined to endocrine cells of the intestine. Falck-Hillarp fluorescence histochemistry revealed 5-HT in endocrine cells and catecholamines in nerve fibres (and possibly also in endocrine cells) throughout the gut. Bombesin-, VIP-, gastrin/CCK- and somatostatin-like immunoreactivities and catecholamine fluorescence were present in nerve fibres of the rectal gland and, with the exception of gastrin/CCK-like immunoreactivity, also in nerve bundles in the walls of the coeliac and mesenteric arteries. The findings of the present study form an anatomical basis for the assumption that several of the neuropeptides and amines could function as neurotransmitters or neuromodulators in the gut of Squalus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00218654
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  • 8
    Electronic Resource
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    Serotonin axons of the ependyma and circumventricular organs in the forebrain of the guinea pig (1983)
    Springer
    Cell & tissue research 228 (1983), S. 297-311 
    Details
    ISSN: 1432-0878
    Keywords: Serotonin ; Supraependymal axons ; Circumventricular organs ; Forebrain ; Immunohistochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The distribution of supraependymal nerve fibers (SEF) containing serotonin (5-HT) was investigated immunohistochemically in the forebrain of the guinea pig. The highest densities of immunoreactive axons were found in the pars centralis and the inferior horn of the lateral ventricle and also in the superior part of the third ventricle. Because of the special development of the choroid plexus in these ventricular regions, it is suggested that 5-HT SEF might be involved in the regulation of the composition of the cerebrospinal fluid. The ependyma lining the circumventricular organs located in the forebrain, was not observed to receive a significant 5-HT-SEF innervation. In the pituitary gland, a loose but constant network of 5-HT axons, resembling those which course in the anterobasal hypothalamus, arcuate nucleus and internal layer of the median eminence, was observed in the neural lobe. In the epiphysis, immunoreactive 5-HT was detected in all pinealocytes (the entire cell was filled with reaction product) and in fibers running between them.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00204880
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  • 9
    Electronic Resource
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    Substance P-containing nerve fibres in large peripheral blood vessels of the rat (1983)
    Springer
    Cell & tissue research 229 (1983), S. 411-422 
    Details
    ISSN: 1432-0878
    Keywords: Substance P ; Neuropeptides ; Immunohistochemistry ; Vascular smooth muscle
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Substance P-immunoreactive nerve fibres were localized by the indirect immunohistochemical method in the adventitia and the adventitial-medial border of large peripheral arteries and veins of the rat. Arteries showed a richer substance P-containing innervation than veins. The superior mesenteric artery was densely innervated, whereas no substance P-containing fibres were found around the carotid artery. Substance P produced a vasoconstriction of the veins, but was basically without effect on arteries, although with the carotid artery a dose-dependent relaxation was observed. The absence of a correlation between the degree of innervation of the blood vessels and their responsiveness to exogenous substance P suggests that these nerves do not subserve a vasomotor function. The depletion of substance P immunoreactivity from nerves in arteries and veins by capsaicin suggests that substance P-containing vascular nerves are primarily sensory in nature.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00214982
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  • 10
    Electronic Resource
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    Differences in the distributional pattern of CRF-, oxytocin-, and vasopressin-immunoreactive nerve fibers in the median eminence of the rat (1983)
    Springer
    Cell & tissue research 230 (1983), S. 247-258 
    Details
    ISSN: 1432-0878
    Keywords: CRF ; Oxytocin ; Vasopressin ; Immunohistochemistry ; Median eminence ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Conspicuous differences in the distributional pattern of nerve fibers containing corticotropin-releasing factor (CRF) or posterior lobe hormones, respectively, were shown in the median eminence of the adult male rat by means of immunoperoxidase histochemistry, with the use of anti-CRF, anti-oxytocin, and anti-vasopressin sera. In the rostral and central divisions of the median eminence, a high concentration of CRF-immunoreactive nerve fibers was found in the median portion of the external layer; these fibers terminated on the capillary loops of the hypophysial portal system. In the caudal division of the median eminence, the CRF-immunoreactive nerve fibers were located in the median to paramedian portions of the external layer. Numerous oxytocin- and vasopressin-immunoreactive nerve fibers were observed evenly distributed throughout the internal layer of the median eminence. In the external layer, a small number of the oxytocin- and vasopressin-containing nerve fibers was found around the capillary loops, particularly in the median to paramedian portions. The distributional patterns of the CRF and the posterior lobe hormones in the hypothalamo-hypophysial system and their functional interrelationship are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00213803
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  • 11
    Electronic Resource
    Electronic Resource
    Association of substance-P-immunoreactive nerves with the murine olfactory mucosa (1983)
    Springer
    Cell & tissue research 230 (1983), S. 517-525 
    Details
    ISSN: 1432-0878
    Keywords: Substance P ; Olfactory mucosa ; Nerve ; Capsaicin ; Immunohistochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Location and distribution of nerve fibers immunoreactive to substance P were studied in the mouse olfactory mucosa. A moderately dense plexus of fibers is present at the interface of the olfactory epithelium and the connective tissue of the lamina propria. In addition, many immunoreactive nerve fibers are noted in close association with Bowman's glands and blood vessels in the lamina propria. However, such fibers were not observed in olfactory epithelium proper nor in the fila olfactoria. Substance-P-immunoreactivity is almost totally abolished by treatment of animals with capsaicin, an agent known to deplete substance P from primary sensory neurons. It is suggested that the substance-P-immunoreactive fibers are of sensory origin, with their perikarya most likely located in the trigeminal ganglia. Functionally, they might influence local blood flow and/or the secretion of Bowman's glands.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00216198
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  • 12
    Electronic Resource
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    Identification of serotonin- and vasopressin immunoreactivities in the suprachiasmatic nucleus of four mammalian species (1983)
    Springer
    Cell & tissue research 234 (1983), S. 237-248 
    Details
    ISSN: 1432-0878
    Keywords: Serotonin ; Vasopressin ; Immunohistochemistry ; Suprachiasmatic nucleus ; Mammals (rat, hamster, cat, Macaca fuscata)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The distribution of serotonin- and vasopressin immunoreactivities in the suprachiasmatic nucleus (SCN) of four mammalian species was studied with the use of the modified peroxidase-antiperoxidase (PAP) method and antisera to serotonin and vasopressin. In the SCN of the rat, hamster and cat, we noted a large number of serotoninimmunoreactive nerve fibers particularly in the ventral area, where these fibers containing small varicosities (less than 1μm in diameter) formed a dense plexus. In the monkey (Macaca fuscata), however, only few serotonin-containing fibers were evident throughout the SCN. Vasopressin-immunoreactive somata and fibers were distributed in large numbers in the SCN of the rat, hamster, cat and monkey, especially in the dorsal nuclear area. Regional and species-related differences of serotonin- and vasopressin distribution in the SCN were elucidated; possible functional differences between the ventral and dorsal areas of the SCN are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00213766
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  • 13
    Electronic Resource
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    Immunohistochemical study on the localization of serotonin fibers and terminals in the spinal cord of the monkey (Macaca fuscata) (1983)
    Springer
    Cell & tissue research 229 (1983), S. 23-36 
    Details
    ISSN: 1432-0878
    Keywords: Serotonin fibers ; Spinal cord ; Immunohistochemistry ; Monkey (Macaca fuscata)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A modified procedure of PAP-immunohistochemistry with the use of a rabbit antiserum against serotonin was applied to investigate the pattern of serotonin-containing nerve fibers in the spinal cord of the monkey, Macaca fuscata. The majority of descending serotonin fibers in the white matter is located immediately below the pia mater in the ventrolateral funiculi. Lamina I and the outer zone of lamina II are supplied with numerous serotonin fibers. In the intermediate gray, two prominent bundles composed of longitudinal fibers, i.e., lateral and medial longitudinal serotonin bundles, were recognized at the lateral column and in the vicinity of the central canal, respectively. The motoneurons of the anterior horn are encompassed by fine networks of serotonin fibers and terminals. The results obtained from studies with the monkey spinal cord closely resemble those characteristic of the dog spinal cord as presented in a previous paper, except for portions of the lumbar level. In segments L3–L4, intercalated cell groups between the medial and lateral motor nuclei receive particularly rich inputs of serotonin fibers in the same manner as the neurons of the nucleus intermediolateralis. This peculiar finding may suggest the presence of a specialized nucleus in the anterior column of the simian and also human spinal cord.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00217878
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  • 14
    Electronic Resource
    Electronic Resource
    Structure of the pars distalis in the adult tammar wallaby (Macropus eugenii) (1983)
    Springer
    Cell & tissue research 229 (1983), S. 155-174 
    Details
    ISSN: 1432-0878
    Keywords: Pituitary ; Tammar wallaby ; Marsupialia ; Ultrastructure ; Immunohistochemistry ; Cell types
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary An immunohistochemical, light- and electron-microscopial study was made of the pars distalis in adult tammar wallabies (Macropus eugenii). The pars distalis of this marsupial mammal was divided into three regions, based on the distribution of cell types within the gland. Somatotropic, mammotropic, luteotropic, folliculotropic, corticotropic and thyrotropic cells were identified on the basis of their immunohistochemistry, cytology and ultrastructure. Non-granulated (folliculo-stellate) cells, identified in electron micrographs, were found throughout the pars distalis. Somatotropic cells were predominant in the posterior pars distalis in all animals examined. In the single male specimen and in the non-lactating females examined, small numbers of apparently inactive mammotropic cells were scattered throughout the pars distalis; the same cell type was apparently active and present in considerable numbers in lactating females. Only one morphological type of gonadotropic cell was evident; these cells were scattered throughout the pars distalis, but in largest numbers in the median region. Small numbers of thyrotropic cells were found, most commonly in the anterior pars distalis. Corticotrops were also observed in moderate numbers, predominantly in the anterior regions of the pars distalis.
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    URL: http://dx.doi.org/10.1007/BF00217888
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  • 15
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    Immunohistochemical identification of neurons containing corticotropin-releasing factor in the rat hypothalamus (1983)
    Springer
    Cell & tissue research 230 (1983), S. 239-246 
    Details
    ISSN: 1432-0878
    Keywords: CRF neurons ; Hypothalamus ; Immunohistochemistry ; PAP ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A specific rabbit anti-CRF serum and the immunoperoxidase technique were used to show that CRF-containing neurons are mainly distributed in the paraventricular and supraoptic nuclei of the rat hypothalamus. In addition, immunoreactive neurons are scattered in other hypothalamic regions. These neurons are 20–30 μm in diameter. From the present and previous investigations it may be concluded that the hypothalamic magnocellular nuclei, i.e., paraventricular and supraoptic, and other hypothalamic accessory nuclei, are the producing sites not only for vasopressin and oxytocin, but also for corticotropin-releasing factor.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00213802
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  • 16
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    Differing postnatal development of the somatostatin- and luliberin- systems in the male and female rat (1983)
    Springer
    Cell & tissue research 232 (1983), S. 357-378 
    Details
    ISSN: 1432-0878
    Keywords: Somatostatin ; Luliberin (LRF) ; Postnatal development (rat) ; Immunohistochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary By means of light-microscopic immunohistochemistry the perikarya of the luliberin-(LRF-) and somatostatin systems of neonate rats were found to be in differing stages of development. At a time point when the LRF-producing neurons had obviously attained their final shape and size, the somatostatin-immunoreactive perikarya were still in a postnatal phase of maturation. Whereas the number of the latter perikarya increases with advancing age, the number of LRF-immunoreactive perikarya decreases significantly from postnatal day 7 onward. Both peptide-hormone systems do not project concomitantly and to the same extent to their principal neurohemal regions in the organum vasculosum laminae terminalis (OVLT) and the median eminence (ME). In all presently studied stages of development, despite considerable individual variations in one age group, among the components of the LRFsystem the OVLT displays a more intense immunoreactivity than the ME. The somatostatin system, however, projects to the OVLT with a conspicuous temporal delay compared to the ME, and, furthermore, in the OVLT the pattern of immunoreactivity characteristic of adult rats is not yet attained at postnatal day 21. Evidence for differences in the immunoreactivity between male and female animals was restricted to the LRF-system. Finally, the results obtained on the stria terminalis speak in favour of the fact that the long-range extrahypothalamic projections of the somatostatin system also undergo postnatal maturation. In the stria terminalis, somatostatin-immunoreactive fibers can be demonstrated initially on postnatal day 7. They attain their full immunoreactivity on postnatal day 21. Furthermore, in the bed nucleus of the stria terminalis an intermittent cytoplasmic immunoreactivity is observed, which is limited to the animals of postnatal day 7 and disappears completely during the further course of development.
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    URL: http://dx.doi.org/10.1007/BF00213793
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  • 17
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    Structure of the pars distalis in pouch-young tammar wallabies (Macropus eugenii) (1983)
    Springer
    Cell & tissue research 230 (1983), S. 587-603 
    Details
    ISSN: 1432-0878
    Keywords: Pituitary ; Tammar wallaby ; Marsupial ; Neonate ; Ultrastructure ; Immunohistochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The pars distalis of pouch-young wallabies (Macropus eugenii) aged 1 to 50 days was studied by means of light-microscopic immunohistochemistry and electron microscopy. In the pars distalis of these pouch-young presumptive somatotrops, which constituted up to 70% of the gland, and nongranulated cells were the most numerous cell types. Small numbers (together representing less than 30% of the pars distalis) of immunoreactive mammotrops, thyrotrops, gonadotrops and corticotrops were also found. The presumptive mammotrops, gonadotrops and thyrotrops increased in number and apparent activity between 1 and 50 days postpartum. Presumptive corticotrop cells in 25 to 30 day-old animals were relatively most numerous, and apparently more active than at any other stage of pouch life; these cells decreased in apparent activity and relative number in older animals. The changes in number and activity of cell types in the pars distalis correlated well with major developmental events such as the onset of adrenal activity, the rapid growth phase in the first 100 days postpartum, and the generally low thyroid activity in pouch-young of less than 50 days of age.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00216203
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  • 18
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    Immunohistochemical investigations of neuropeptides in the brain, corpora cardiaca, and corpora allata of an adult lepidopteran insect, Manduca sexta (L) (1983)
    Springer
    Cell & tissue research 232 (1983), S. 295-317 
    Details
    ISSN: 1432-0878
    Keywords: Insect brain ; Corpus cardiacum/corpus allatum ; Neuropeptides ; Immunohistochemistry ; Tobacco hornworm
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In the brain of adult specimens of the tobacco hornworm moth, Manduca sexta (L), cells immunoreactive for several kinds of neuropeptides were localized by means of the PAP procedure, by use of antisera raised against mammalian hormones or hormonal peptides. In contrast, no such neurosecretory cells were found in the corpora cardiaca and corpora allata (CC/CA); in the CC/CA, however, immunoreactive nerve fibres were observed, reaching these organs from the brain. The neurosecretory cells found in the brain were immunoreactive with at least one of the following mammalian antisera, namely those raised against the insulin B-chain, somatostatin, glucagon C-terminal, glucagon N-terminal, pancreatic polypeptide (PP), secretin, vasoactive intestinal polypeptide (VIP), glucose-dependent insulinotropic peptide (GIP), gastrin C-terminus, enkephalin, α-and β-endorphin, Substance P, and calcitonin. No cells were immunoreactive with antisera specific for detecting neurons containing the insulin A-chain, nerve growth factor, epidermal growth factor, insulin connecting peptide (C-peptide), polypeptide YY (PYY), gastrin mid-portion (sequence 6–13), cholecystokinin (CCK) mid-portion (sequences 9–20 and 9–25), neurotensin C-terminus, bombesin, motilin, ACTH, or serotonin. All the neuropeptide-immunoreactive cells observed emitted nerve fibers passing through the brain to the CC and in some cases also to the CA. In CC these immunoreactive nerve fibers tended to accumulate near the aorta. It was speculated that neuropeptides are released into the circulating haemolymph and act as neurohormones.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00213788
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  • 19
    Electronic Resource
    Electronic Resource
    Morphological studies on neuroglia (1983)
    Springer
    Cell & tissue research 229 (1983), S. 85-95 
    Details
    ISSN: 1432-0878
    Keywords: Amoeboid microglial cells ; Brain macrophages ; Immunohistochemistry ; Macrophage antiserum ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Immunohistochemical studies with the use of the peroxidase-antiperoxidase (PAP) method revealed that “amoeboid microglial cells”, in the brains of neonatal rats and “brain macrophages” in lesioned brains of adult rats react positively to an antiserum raised against macrophages. In brains of neonatal rats, “amoeboid microglial cells” stained by means of the PAP-method were observed in the corpus callosum, internal capsule, dorso-lateral region of the thalamus, subventricular zone of the lateral ventricle, and the subependymal layer of the ventricular system. These cellular elements were not detected in brains of rats aged 21 days or older. Resting microglial cells displaying a typical ramified structure were not specifically stained. Cells reacting positively to the macrophage antiserum appeared (i) in the cerebral cortex of adult rats following placement of a stab wound, or (ii) in the hippocampal formation after kainic acid-induced lesions; in the damaged areas immunoreactive cells exhibited the typical features of “brain macrophages”. “Brain macrophages” and “amoeboid microglial cells” are considered to belong to the class of exudate macrophages derived from blood monocytes. Thus, elements of hematogenous origin do exist in the intact brain parenchyma of neonatal rats and in lesioned brains of adult rats. The relationship between brain macrophages and resting microglial cells is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00217882
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  • 20
    Electronic Resource
    Electronic Resource
    Immunohistochemical demonstration of serotonin-containing nerve fibers in the inferior olivary complex of the rat, cat and monkey (1983)
    Springer
    Cell & tissue research 231 (1983), S. 17-28 
    Details
    ISSN: 1432-0878
    Keywords: Serotonin-immunoreactive nerve fibers ; Inferior olivary complex ; Immunohistochemistry ; Rat, cat, monkey
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary By the use of a modified peroxidase-antiperoxidase immunohistochemical method, the distribution of serotonin-containing nerve fibers was studied in the inferior olivary complex of the rat, cat and monkey. The entire inferior olivary complex of all three species contained serotonin fibers, and species-related differences in the distribution of serotonin fibers were observed. In the rat, the overall density of serotonin fibers was sparser than that in the other two species; the highest concentration of varicose serotonergic fibers was noted in the lateral portion of the dorsal accessory olive. In the cat, the densest distribution of serotonin fibers occurred in the caudal portion of the medial accessory olive, the dorsomedial cell column and the lateral portion of the dorsal accessory olive, where intervaricose segments of serotonin-immunoreactive fibers were not so distinct. In the monkey, the caudal medial accessory olive, the lateral portion of the dorsal accessory olive and the dorsal as well as the lateral lamella of the principal olive showed a maximum density of serotonin fibers. Apart from the influence of afferent serotonergic projections to the cerebellum, serotonergic neurons of the brainstem are considered to affect Purkinje cells via neurons of the inferior olivary complex projecting to the cerebellum particularly in the cat and monkey.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00215770
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