ALBERT

All Library Books, journals and Electronic Records Telegrafenberg

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
Filter
  • Books
  • Articles  (43)
  • Zea  (22)
  • Autoradiography  (21)
  • Springer  (43)
  • Oxford University Press
  • 1980-1984  (43)
  • 1960-1964
  • 1981  (43)
Collection
  • Books
  • Articles  (43)
Source
Keywords
Publisher
Years
  • 1980-1984  (43)
  • 1960-1964
Year
Topic
  • 1
    Electronic Resource
    Electronic Resource
    Incorporation of35S-sulphate in chick blastoderms during elongation and during shortening of the primitive streak (1981)
    Springer
    Development genes and evolution 190 (1981), S. 233-236 
    Details
    ISSN: 1432-041X
    Keywords: Sulphate ; Glycosaminoglycan ; Autoradiography ; Gastrulation ; Chick
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Chick blastoderms were cultured for 2 h in the presence of35S-sulphate. The distribution of the grains after light microscope autoradiography was compared in blastoderms during the elongation and during the shortening of the primitive streak. A uniform labeling was observed over the cells in both groups. Accumulation of grains was present in both groups at the ventral side of the upper layer, where transmission electron microscope studies have revealed a basal lamina. An additional accumulation of grains occurred over the cells and in the extracellular spaces of the head process and of the rostral part of blastoderms with shortening primitive streaks. This positivity could be correlated with the presence of ingressing and recently ingressed notochordal cells. Treatment of the sections with chondroitinase ABC and/or HNO2 before dipping in the nuclear emulsion demonstrated that at least chondroitin sulphate and N-sulphated heparan sulphate were present.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00848309
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Auxin uptake and action of N-1-naphthylphthalamic acid in corn coleoptiles (1981)
    Springer
    Planta 151 (1981), S. 15-25 
    Details
    ISSN: 1432-2048
    Keywords: Auxin (uptake, transport) ; Benzoic acid ; N-1-Naphthylphthalamic acid ; Zea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The validity of a chemiosmotic hypothesis for uptake of weak acids as an explanation for the accumulation of auxin by cells has been explored further by comparing the uptake of indole-3-acetic acid (IAA) by 1-mm segments of corn (Zea mays L.) coleoptiles with that of benzoic acid and two neutral indoles, indoleethanol and indoleacetonitrile, which do not ionize. These substances, while structurally related to IAA lack both auxin activity and polar transport. Uptake of IAA and benzoic acid increase with decreasing external pH, whereas the uptake of the two neutral indoles is independent of external pH. Although metabolism of IAA, during 90 min or less, is minimal and without significant effect on its uptake, metabolism of benzoic acid appears responsible for the apparent saturation of benzoic acid uptake at high concentrations. An inhibitor of auxin transport, N-1-naphthylphathalamic acid (NPA), stimulates uptake of IAA but has no effect on uptake of either benzoic acid or the two neutral indoles. Thus, NPA does not affect the driving forces for accumulation of weak acids but probably specifically decreases the flux of the auxin anions relative to undissociated auxin. Since the electrochemical potential of auxin anions is usually higher in than outside cells, blocking the anion flux with NPA would enhance auxin uptake. Azide, which abolishes accumulation of both IAA and benzoic acid, may simply collapse the pH gradient across the plasma membrane. In the absence of NPA, increasing concentrations of auxins or the analogoue β-naphthaleneacetic acid (β-NAA) exert two opposing effects on the uptake of IAA-depression and stimulation. Stimulation results from saturating the anion flux. With uptake fully stimulated by NPA, however, increasing concentrations of auxins or analogues only depress uptake of [3H]IAA. These results are consistent with more than one path for auxin transport each with a different dependence on concentration. In depressing NPA-stimulated IAA uptake, the effectiveness of β-NAA≧IAA≫α-NAA≫ benzoic acid, a specificity similar to that of an auxin binding site in vitro that has been implicated by others in auxin transport. The results support the general hypothesis that cellular auxin uptake and polar transport through tissues are chemiosmotically coupled to the electrochemical potential of auxin and protons.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00384232
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Isolation and characterization of aminopterin-resistant cell lines in maize (1981)
    Springer
    Planta 153 (1981), S. 436-442 
    Details
    ISSN: 1432-2048
    Keywords: Aminopterin-resistant variants ; Cell culture ; Folate analog ; Variant selection ; Zea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Aminopterin-resistant cell lines of maize were isolated by two different procedures of callus selection and by plating suspension cultures on drugcontaining medium after mutagen treatment. Efficiencies of different methods of variant selection were compared. Four aminopterin-resistant cell lines were shown to be 10–40 times more resistant than the parental cell line, and they were also resistant to another folate analog, methotrexate. The results suggest that alterations in at least three different cell properties could be responsible for resistance; 1) increased dihydrofolate reductase activity, 2) altered aminopterin sensitivity of dihydrofolate reductase, and 3) reduced drug uptake. One of the resistant cell lines showed more than one alteration, but its resistance proved to be unstable. The results suggest that stable changes which may or may not be of genetic origin and also unstable physiological changes or a combination of both could lead to aminopterin resistance in maize cell cultures.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00394982
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    Effect of zearalenone (F-2) on pea stem, maize root, and rat liver mitochondria (1981)
    Springer
    Planta 153 (1981), S. 443-446 
    Details
    ISSN: 1432-2048
    Keywords: ATPase ; Mitochondria ; Mycotoxin ; Pisum ; Zea ; Zearalenone
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract At 5 and 10 μg ml-1 concentration, zearalenone (F-2), a mycotoxin produced by a number of species of the genus Fusarium, causes an inhibition of the oxidative phosphorylation of isolated plant mitochondria, while at 20 and 40 μg ml-1 it causes uncoupling. However, when the mitochondria are pre-incubated for 20 min with F-2, the uncoupling appears to be the prevailing effect. F-2 is also able to inhibit the mitochondrial ATPase activity (Mg2+-dependent). Conversely, F-2 (40 μg ml-1) does not alter the ATP level of maize roots and only slightly affects the ATPase activity of pea stem and maize root microsomal fractions. In addition, F-2 (10–40 μg ml-1) inhibits ATP synthesis catalyzed by rat liver mitochondria. It is suggested that the phytotoxicity of F-2, also known for its ability to collapse the transmembrane electric potential of maize roots, may be mainly linked to its ability to increase the proton permeability of the cell, similar to the common uncouplers.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00394983
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 5
    Electronic Resource
    Electronic Resource
    Light-induced inhibitors from intact and cultured caps of Zea roots (1981)
    Springer
    Planta 153 (1981), S. 471-475 
    Details
    ISSN: 1432-2048
    Keywords: Abscisic acid ; Geotropism (root) ; Growth inhibitor ; Light and root-cap inhibitor ; Root cap (inhibitor) ; Zea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Growth inhibitors were assayed from extracts of intact (attached) and of excised (cultured) root caps of Zea mays L., cv. Merit, the roots of which show a positive geotropic response only after exposure to light. If caps are intact at the time of illumination, at least two inhibitory substances are produced, an acid inhibitor and a neutral inhibitor, whereas if caps are detached from roots, placed in culture and then illuminated only the neutral inhibitor is formed. Cycloheximide retards inhibitor production in both intact and cultured caps. When [14C]mevalonic acid is included in the culture medium and the caps are illuminated, 15–25% of the recoverable 14C cochromatographs with the neutral inhibitor, whereas in caps cultured in the dark, this radiolabelling pattern is not observed. Cyloheximide in the light reduces the incorporation of 14C into compounds cochromatographing at the Rf of the neutral inhibitor. It is suggested that the neutral inhibitor may be important in the light-induced bending of roots.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00394989
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 6
    Electronic Resource
    Electronic Resource
    Levels of indole-3-acetic acid in intact and decapitated coleoptiles as determined by a specific and highly sensitive solid-phase enzyme immunoassay (1981)
    Springer
    Planta 153 (1981), S. 561-571 
    Details
    ISSN: 1432-2048
    Keywords: Auxin (immunoassay) ; Avena ; Coleoptiles (physiological tip) ; Enzyme immunoassay ; Zea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A specific solid-phase enzyme immunoassay for the detection of as little as 3–4 pg of indole-3-acetic acid (IAA) is described. The assay involves minimal procedural efforts and requires only standard laboratory equipment. Up to 50 samples in triplicate, processed simultaneously, can be assayed and evaluated in 2.5 h. As little as 1 mg oat coleoptile tissue is sufficient for a quantitative IAA analysis and little or no extract purification is necessary. Using this assay, levels of IAA have been determined in coleoptiles of maize and oat. The distribution of IAA within single coleoptiles was quantitated and the production of IAA during the regeneration of the physiological tip in Avena coleoptiles was investigated. The changes in levels of IAA and other major phytohormones were quantitated during the growth of oat coleoptiles.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00385542
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 7
    Electronic Resource
    Electronic Resource
    Partial purification of endo- and exo-β-D-glucanase enzymes from Zea mays L. seedlings and their involvement in cell-wall autohydrolysis (1981)
    Springer
    Planta 151 (1981), S. 206-214 
    Details
    ISSN: 1432-2048
    Keywords: Cell-wall autohydrolysis ; β-D-Glucan ; Glucanase ; Hemicellulose ; Zea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The proteins dissociated from isolated Zea seedling cell wall using high-ionic-strength salt solutions have been found to include a number of enzymes which appear to participate in autolytic reactions of the cell wall. These enzymes caused extensive degradation of enzymatically inactive cell wall, liberating as much as 100 μg/mg dry weight over a 48-h period. Lithium chloride (3M) was shown to be most effective in yielding protein and wall-degrading activities. Molecular-sieve chromatography of the cell-wall protein resolved endo-β-D-glucanase and exo-β-1,3-glucanase (EC 3.2.1.58) activities when Avena glucan and laminarin, respectively, were employed as substrates. The exoenzyme (molecular weight around 60,000) was strongly inhibited by inorganic mercury at a concentration which suppressed the release of monosaccharide from autolytically active cell wall. The endo-β-D-glucanase (MW around 26,000), which showed a marked preference for substrates of mixed-linkage, exhibited features indicating that it initiates the autolytic solubilization of wall glucan. Cell-wall β-D-glucan, recovered as a component of an alkali-soluble cell-wall fraction, served as a substrate for the purified glucanases. Their hydrolysis pattern, assessed using gel exclusion chromatography and product analysis, confirmed that they hydrolyze β-D-glucan. The products generated by the endoglucanase were similar in molecular-size distribution to those liberated from autolytically active-wall. Exoglucanase activity was required for extensive hydrolysis of β-D-glucan in vitro. During coleoptile development the autolytic activity of the cell wall increased dramatically. This increased activity, however, did not parallel the growth potential of the tissue, but more closely reflected an increase in cell-wall β-D-glucan, the primary substrate for autolytic reactions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00395171
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 8
    Electronic Resource
    Electronic Resource
    Acid growth effects in maize roots: Evidence for a link between auxin-economy and proton extrusion in the control of root growth (1981)
    Springer
    Planta 152 (1981), S. 285-291 
    Details
    ISSN: 1432-2048
    Keywords: Acid growth ; Auxin ; Growth ; Root ; Zea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The role of proton excretion in the growth of apical segments of maize roots has been examined. Growth is stimulated by acidic buffers and inhibited by neutral buffers. Organic buffers such as 2[N-morpholino] ethane sulphonic acid (MES) — 2-amino-2-(hydroxymethyl)propane-1,3 diol (Tris) are more effective than phosphate buffers in inhibiting growth. Fusicoccin(FC)-induced growth is also inhibited by neutral buffers. The antiauxins 4-chlorophenoxyisobutyric acid (PCIB) and 2-(naphthylmethylthio) propionic acid (NMSP) promote growth and H+-excretion over short time periods; this growth is also inhibited by neutral buffers. We conclude that growth of maize roots requires proton extrusion and that regulation of root growth by indol-3yl-acetic acid (IAA) may be mediated by control of this proton extrusion.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00388251
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 9
    Electronic Resource
    Electronic Resource
    Effects of high temperature on the germination of maize (Zea mays L.) (1981)
    Springer
    Planta 151 (1981), S. 68-74 
    Details
    ISSN: 1432-2048
    Keywords: Energy metabolism ; Germination (seeds) ; Protein synthesis ; Temperature and seed germination ; Zea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Poor emergence of maize seedlings, due to high soil temperatures, is a major limitation of crop potential in the lowland tropics. Ability to germinate at high temperature (〉c. 37° C) is related to the temperature sensitivity of the embryo, and there is considerable genotypic variation for this character. Respiration and mitochondrial phosphorylation proceed normally in seeds imbibing at 41° C, and ATP levels are adequate for germination. However, the specific activities of several important enzymes are lower, and the rate of protein synthesis is severely reduced compared with seeds imbibing at 28° C. The depression of the rate of protein synthesis in the embryos of several tropical hybrids imbibing at high temperature correlated with their known temperature sensitivity. It is concluded that protein synthesis is an especially temperature sensitive process in germinating maize embryos, and that this is the principal reason for the sensitivity of germinating maize seeds to high temperature.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00384239
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 10
    Electronic Resource
    Electronic Resource
    Effects of high temperature on protein synthesis during germination of maize (Zea mays L.) (1981)
    Springer
    Planta 151 (1981), S. 75-80 
    Details
    ISSN: 1432-2048
    Keywords: Embryos (protein synthesis) ; Germination (seeds) ; Protein synthesis and temperature ; Temperature and protein synthesis ; Zea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The poor germination of maize seeds at high temperatures (〉37°C) is related to the low rate of protein synthesis by the embryo. The apparatus of translation was not heat-labile when embryos were incubated for 2 h at 41°C, and cell free extracts from seeds imbibed for 16 h at this temperature were able to translate exogenous mRNA, indicating that ribosomes and other subcellular components were present and functional. Analysis of polysome profiles from embryos imbibing at high temperature indicated that the low rate of protein synthesis was due to the non-availability of active mRNA.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00384240
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 11
    Electronic Resource
    Electronic Resource
    Uptake of isolated plant chromosomes by plant protoplasts (1981)
    Springer
    Planta 151 (1981), S. 141-145 
    Details
    ISSN: 1432-2048
    Keywords: Chromosome isolation ; Petroselinum ; Polyethylene-glycol ; Protoplast (uptake of chromosomes) ; Triticum ; Zea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract For mass isolation of plant metaphase chromosomes, cultured cells of wheat (Triticum monococcum) and parsley (Petroselinum hortense) were synchronized by hydroxyurea and colchicine treatment. This synchronization procedure resulted in high mitotic synchrony, especially in suspension cultures of parsley in which 80% of the cells were found to be at the metaphase stage. Mitotic protoplasts isolated from these synchronized cell cultures served as a source for isolation of chromosomes. The described isolation and purification method yielded relatively pure chromosome suspension. The uptake of the isolated plant chromosomes into recipient wheat, parsley, and maize protoplasts was induced by polyethylene-glycol treatment. Cytological studies provided evidences for uptake of plant chromosomes into plant protoplasts.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00387815
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 12
    Electronic Resource
    Electronic Resource
    Differential growth of georeacting maize roots (1981)
    Springer
    Planta 151 (1981), S. 146-150 
    Details
    ISSN: 1432-2048
    Keywords: Georeaction (root) ; Growth inhibitors ; Growth (root) ; Root (georeaction, growth) ; Zea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The growth rate of the two sides of 10-mm apical segments prepared from primary roots and of intact primary roots of maize has been analyzed in both vertical and horizontal positions, using a filming method allowing continuous growth recording. The data showed that the georeaction began by a decrease in the overall elongation rate of the roots. This inhibition is effective on the lower side of the bending zone, where the growth is practically stopped during the period of maximum rate of geocurvature. In contrast, the growth is slightly enhanced on the upper part of the elongating zone.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00387816
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 13
    Electronic Resource
    Electronic Resource
    Daily changes in nitrate influx, efflux and metabolism in maize and pearl millet (1981)
    Springer
    Planta 152 (1981), S. 319-324 
    Details
    ISSN: 1432-2048
    Keywords: Nitrate influx (efflux, metabolism) ; Pennisetum ; Zea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Maize (Zea mays L.) and pearl millet (Pennisetum americanum (L.) Leeke) seedlings were exposed to [15N]nitrate for 1-h periods at eight times during a 24-h period (16–8 h light-dark for maize; 14–10 h for millet). Influx of [15N]nitrate as well as its reduction and translocation were determined during each period. The efflux of previously absorbed [14N]nitrate to the uptake solution was also estimated. No marked diurnal changes in [14N]nitrate efflux or [15N]nitrate influx were evident in maize. In contrast, [14N]nitrate efflux from millet increased and eventually exceeded [15N]nitrate influx during the late dark and early light periods, resulting in net nitrate efflux from the roots. The dissimilarity of their diurnal patterns indicates that influx and efflux are independently regulated. In both species, [15N]nitrate reduction and 15N translocation to shoots were curtailed more by darkness than was [15N]nitrate influx. In the light, maize reduced 15% and millet 24% of the incoming [15N]nitrate. In darkness, reduction dropped to 11 and 17%, respectively. Since the accumulation of reduced-15N in shoots declined abruptly in darkness, whereas that in roots was little affected, it is suggested that in darkness [15N]nitrate reduction occurred primarily in roots. The decrease in nitrate uptake and reduction in darkness was not related to efflux, which remained constant in maize and did not respond immediately to darkness in pearl millet.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00388256
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 14
    Electronic Resource
    Electronic Resource
    Energetics of the response of maize coleoptile tissue to indoleacetic acid (1981)
    Springer
    Planta 151 (1981), S. 499-505 
    Details
    ISSN: 1432-2048
    Keywords: Auxin ; Calorimetry ; Coleoptile ; Energetics ; Growth regulator ; Zea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Indole-3-acetic acid (IAA) promotes an increase in steady-state heat production by corn (Zea mays L.) coleoptile tissue; this increase is associated with an elevation in aerobic respiration rates. A detailed time dependence of the exothermic response to IAA was obtained using flow calorimetry. The latent period and magnitude of response were evaluated as a function of IAA concentration and pH. The data indicate that more than one response may occur. The optimal change in heat production was produced by an IAA concentration of 3·10-5 M. It was initiated within 5 min after the start of the IAA treatment, and reached a magnitude in excess of 25% of the tissue's basal heat production. Concentrations of IAA greater than 1·10-4 M resulted in diminished response(s), but the effect was strongly pH dependent. Several possibilities for the increased heat production triggered by IAA are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00387426
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 15
    Electronic Resource
    Electronic Resource
    A possible plasma membrane particle containing malic enzyme activity (1981)
    Springer
    Planta 151 (1981), S. 506-511 
    Details
    ISSN: 1432-2048
    Keywords: Cell fractionation ; Cucurbita ; Malic enzyme ; Plasma membrane ; Subcellular localization ; Zea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A definite membrane fraction from Cucurbita hypocotyls, maize coleoptiles, and other plant tissues contains a NADP-dependent malic enzyme activity, up to 10% of overall tissue activity, and probably other soluble proteins. This “malic enzyme particle” is identified as plasmalemma on the basis of sedimentation behavior, density distribution in sucrose gradients, in comparison with enzyme markers, and sluggish penetration by the sugar Metrizamide. Enzyme binding to the plasma membrane is stable and scarcely sensitive to salts and EDTA, although all activity is released to the supernatant in the presence of Triton-X-100 or under hypotonic conditions. The properties of bound enzyme are similar to those of free enzyme in cell extracts. It is proposed that osmotically sensitive plasma membrane vesicles, containing cytoplasm fragments, are formed during homogenization. Low malic enzyme activities are also associated with Cucurbita proplastids.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00387427
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 16
    Electronic Resource
    Electronic Resource
    Comparative analyses of the effect of triacontanol on photosynthesis, photorespiration and growth of tomato (C3-plant) and maize (C4-plant) (1981)
    Springer
    Planta 152 (1981), S. 44-49 
    Details
    ISSN: 1432-2048
    Keywords: Lycopersicon ; Phetorespiration ; Photosynthesis ; Triacontanol ; Zea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Tomato (C3-plants) and maize (C4-plants) were grown in a nutrient solution to which triacontanol was added twice a week. After about 4 weeks the triacontanol treatment caused a significant increase in the dry weight of the tomato plants. Leaf area and dry weight measurements of tomato leaves at different stages of development showed that the largest increase in growth was obtained when triacontanol treatment was initiated before bud formation. In maize, no effect of the triacontanol treatment on dry wieght was observed. Photosynthesis was inhibited by 27% in young leaves from triacontanol-treated tomato plants and 39% in the controls, when the oxygen concentration was raised from 2% to 21%. In maize no change in photosynthesis could be observed, neither after altered oxygen concentration nor after triacontanol treatment. The difference in the response of C3- and C4-plants to triacontanol indicates that it regulates processes related to photosynthesis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00384983
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 17
    Electronic Resource
    Electronic Resource
    Extent of intracellular pH changes during H+ extrusion by maize root-tip cells (1981)
    Springer
    Planta 152 (1981), S. 74-78 
    Details
    ISSN: 1432-2048
    Keywords: Cytoplasmic pH ; Fusicoccin ; Hydrogen-ion extrusion ; pH-stat ; Root ; Zea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract 31P-Nuclear-magnetic-resonance spectra of maize (Zea mays L.) root tips, that had been induced to extrude large amounts of H+ in response to fusicoccin (FC) in the presence of potassium salts, indicate that the cytoplasmic pH does not become higher than that of controls. In fact, the cytoplasmic pH may become slightly (approx. 0.1 pH unit) lower in cells extruding H+. Estimations of the buffer capacity of the cells show that without active intracellular pH regulation, H+ extrusion caused by FC would cause the intracellular pH to rise by at least 0.6 pH unit h-1. Our results indicate that intracellular pH is tightly regulated even during extreme rates of acid extrusion, and that a rise in cytoplasmic pH is not the signal linking H+ extrusion with enhanced organic-acid synthesis or other intracellular responses to H+ pumping.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00384988
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 18
    Electronic Resource
    Electronic Resource
    Synthesis of the intranuclear crystals in Scolopendrium vulgare (L.) SM (1981)
    Springer
    Planta 152 (1981), S. 137-144 
    Details
    ISSN: 1432-2048
    Keywords: Autoradiography ; Intranuclear crystals ; Nucleus, crystolo-Protein transfer-Pteridophytes ; Scolopendrium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Proteinaceous intranuclear crystals are found in the fern Scolopendrium vulgare. During mitosis these crystals are eliminated from the nucleus into the cytoplasm, where they are dissolved. New crystals appear in the nucleus. The site of synthesis of intranuclear crystal proteins was investigated using quantitative ultrastructural autoradiography after incubation with tritiated lysine. The results suggest a migration of cytoplasmic proteins to the nucleus, part of which would then be incorporated into the intranuclear crystals.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00391185
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 19
    Electronic Resource
    Electronic Resource
    Correlations between proton-efflux patterns and growth patterns during geotropism and phototropism in maize and sunflower (1981)
    Springer
    Planta 152 (1981), S. 239-241 
    Details
    ISSN: 1432-2048
    Keywords: Acid growth ; Geotropism ; Helianthus ; Phototropism ; Proton secretion ; Zea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract By placing seedlings of sunflower (Helianthus annuus L.) or maize (Zea mays L.) on agar plates containing a pH indicator dye it is possible to observe surface pH patterns along the growing seedling by observing color changes of the indicator dye. Using this method we find that in geotropically stimulated sunflower hypocotyls or maize coleoptiles there is enhanced proton efflux on the lower surface of the organ prior to the initiation of curvature. As curvature develops the pattern of differential acid efflux becomes more intense. A similar phenomenon is observed when these organs are exposed to unilateral illumination, i.e. enhanced acid efflux occurs on the dark side of the organ prior to the initiation of phototropic curvature and the pattern of differential acid efflux intensifies as phototropic curvature develops. These observations indicate that differential acid efflux occurs in response to tropistic stimuli and that the acid efflux pattern may mediate the development of tropistic curvatures.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00385150
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 20
    Electronic Resource
    Electronic Resource
    The action of specific inhibitors of auxin transport on uptake of auxin and binding of N-1-naphthylphthalamic acid to a membrane site in maize coleoptiles (1981)
    Springer
    Planta 152 (1981), S. 13-18 
    Details
    ISSN: 1432-2048
    Keywords: Auxin uptake ; Coleoptile ; Fluorescein ; Morphactin ; Naphthylphthalamic acid ; Triiodobenzoic acid ; Zea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Using both 1-mm segments of corn (Zea mays L.) coleoptiles and a preparation of membranes isolated from the same source, we have compared the effectiveness of several inhibitors of geotropism and polar transport in stimulating uptake of auxin (indole-3-acetic acid, IAA) into the tissue and in competing with N-1-naphthylphthalamic acid (NPA) for a membrane-bound site. Low concentrations of 2,3,5-triiodobenzoic acid (TIBA), NPA, 2-chloro-9-hydroxyfluorene-9-carboxylic acid (morphactin), and fluorescein, eosin, and mercurochrome all stimulated net uptake of [3H]IAA by corn coleoptile tissues while higher concentrations reduced the uptake of both [3H]IAA and another lipophilic weak acid, [14C]benzoic acid. Since low concentrations of fluorescein and its derivatives competed for the same membrane-bound site in vitro as did morphactin and NPA, the basis for both the specific stimulation of auxin accumulation and the inhibition of polar auxin transport by all these compounds may be their ability to interfere with the carrier-mediated efflux of auxin anions from cells. At higher concentrations, the decrease in accumulation of weak acids was nonspecific and thus may be the result of acidification of the cytoplasm and a general decrease in the driving force for uptake of the weak acids. Triiodobenzoic acid was an exception. Low concentration of TIBA (0.1–1 μM) were much less effective than NPA in competing for the NPA receptor in vitro, but little different from NPA in ability to stimulate auxin uptake. One possibility is that TIBA, a substance which is polarly transported, may compete with auxin for the polar transport site while NPA, morphactin, and the fluorescein derivatives may render this site inactive.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00384978
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 21
    Electronic Resource
    Electronic Resource
    Low and high voltage electron microscopy of mitosis and cytokinesis in maize roots (1981)
    Springer
    Planta 152 (1981), S. 397-407 
    Details
    ISSN: 1432-2048
    Keywords: Cytokinesis ; High voltage electron microscopy ; Mitosis ; Zea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The structure and distribution of cytoplasmic membranes during mitosis and cytokinesis in maize root tip meristematic cells was investigated by low and high voltage electron microscopy. The electron opacity of the nuclear envelope and endoplasmic reticulum (ER) was enhanced by staining the tissue in a mixture of zinc iodide and osmium tetroxide. Thin sections show the nuclear envelope to disassemble at prophase and become indistinguishable from the surrounding ER and polar aggregations of ER. In thick sections under the high voltage electron microscope the spindle is seen to be surrounded by a mass of tubular (TER) and cisternal (CER) endoplasmic reticulum derived from both the nuclear envelope and ER, which persists through metaphase and anaphase. At anaphase strands of TER traverse the spindle between the arms of the chromosomes. The octagonal nuclear pore complexes disappear by metaphase, but irregular-shaped pores persist in the membranes during mitosis. It is suggested that these form a template for pore-complex reformation during telophase. Phragmoplast formation is preceded by an aggregation of TER across the spindle at anaphase. Evidence is presented to suggest that the formation of the desmotubule of a plasmodesma is by the squeezing of a strand of endoplasmic reticulum between the vesicles of the cell plate.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00385355
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 22
    Electronic Resource
    Electronic Resource
    Auxin binding in roots: A comparison between maize roots and coleoptiles (1981)
    Springer
    Planta 153 (1981), S. 447-452 
    Details
    ISSN: 1432-2048
    Keywords: Auxin binding ; Root (auxin) ; Zea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Auxin binding onto membrane fractions of primary roots of maize seedlings has been demonstrated using naphth-1yl-acetic acid (NAA) and indol-3yl-acetic acid (IAA) as ligands. This binding is compared with the already well characterized interaction between auxins and coleoptile membranes. The results indicate that while kinetic parameters are of the same order for root and coleoptile binding, a number of differences occur with respect to location in cells and relative affinity. The possible significance of the existence of such binding sites in root cells is discussed in relation to auxin action.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00394984
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 23
    Electronic Resource
    Electronic Resource
    Abscisic acid distribution in horizontal maize root segments (1981)
    Springer
    Planta 153 (1981), S. 453-458 
    Details
    ISSN: 1432-2048
    Keywords: Abscisic acid ; Gravireaction ; Growth inhibitors ; Root ; Zea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Using gas chromatography-mass spectrometry (GC/MS) techniques of analyses, it has been found that endogenous abscisic acid (ABA) becomes asymmetrically distributed in the elongation zone of horizontal Zea mays (cv. LG 11) roots which are showing a positive gravitropic response. There is a relative increase in the ABA content of the lower half and a concomitant decrease for the upper half in such roots. Asymmetric distribution of ABA is also detected in the elongation zone of half-decapped roots.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00394985
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 24
    Electronic Resource
    Electronic Resource
    Inhibition by silver ions of gas space (aerenchyma) formation in adventitious roots of Zea mays L. subjected to exogenous ethylene or to oxygen deficiency (1981)
    Springer
    Planta 153 (1981), S. 217-224 
    Details
    ISSN: 1432-2048
    Keywords: Aerenchyma ; Ethylene ; Gas space ; Oxygen and gas-space formation ; Roots, adventitious ; Silver ions ; Zea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have studied the role of ethylene in accelerating the lytic formation of gas spaces (aerenchyma) in the cortex of adventitious roots of maize (Zea mays L.) growing in poorly aerated conditions. Such roots had previously been shown to contain increased concentrations of ethylene. Ten day-old maize plants bearing seminal roots and one whorl of emerging adventitious roots were grown in nutrient solution bubbled with air, ethylene in air (0.1 to 5.0 μl l−1), or allowed to become oxygen-deficient in nonaerated (but not completely anaerobic) solution. Additions of 0.1 μl l−1 ethylene or more promoted the formation of aerenchyma, with lysis of up to 47% of the cortical cells. The effects of non-aeration were similar to those of exogenous ethylene. When silver ions, an ethylene antagonist, were present at low, non-toxic concentrations (circa 0.6 μM), aerenchyma formation was prevented in ethylene treated roots and in those exposed to oxygen deficiency. Silver ions also blocked the inhibiting effect of exogenous ethylene on root extension. By contrast, the suppression of aerenchyma formation by silver ions under oxygendeficient conditions was associated with a retardation of root extension, indicating the importance of aerenchyma for root growth in poorly aerated media. Rates of production of ethylene by excised roots were stimulated by a previous non-aeration treatment. The effectiveness of Ag+ in inhibiting equally the action on cortical cells of exogenous ethylene and of non-aeration, supports the view that gas space (aerenchyma) formation in adventitious roots ‘adpted’ to oxygendeficient environments is mediated by increased concentrations of endogenous ethylene. The possibility that extra ethylene could arise from increased biosynthesis of a precursor in root tissues with a restricted oxygen supply is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00383890
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 25
    Electronic Resource
    Electronic Resource
    Autoradiographic assessment of 3H-proline uptake by osteoblasts following guanethidine-induced sympathectomy in the rat (1981)
    Springer
    Cell & tissue research 216 (1981), S. 215-220 
    Details
    ISSN: 1432-0878
    Keywords: Autoradiography ; 3H-proline ; Osteoblasts ; Sympathectomy ; Guanethidine-sulfate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Sympathectomy was carried out in rats by injections of guanethidine-sulfate from birth to 14 days of age. At 45 days of age, the activity of osteoblastic cells was monitored by 3H-proline autoradiography. Effectiveness of sympathectomy was verified by light-microscopic examination of superior cervical and celiac ganglia. Grain counts over periosteal osteoblasts of the femoral diaphysis and osteoblasts mesial to the first molar in the mandible demonstrated a significantly reduced uptake of 3H-proline in the sympathectomized rats. The data provide direct evidence of sympathetic influence on osteoblastic activity and suggest that sympathectomy may result in the loss of a trophic influence which is important in the regulation of osteogenesis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00234556
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 26
    Electronic Resource
    Electronic Resource
    Ultrastructural morphometry of blastogenesis (1981)
    Springer
    Cell & tissue research 215 (1981), S. 643-649 
    Details
    ISSN: 1432-0878
    Keywords: T-lymphocytes ; Blast cells ; Autoradiography ; Electron microscopy ; Stereology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary As a companion to an earlier study, the morphometric attributes of stimulated (blast-derived) lymphocytes in mouse axillary lymph nodes have been established using stereological and autoradiographic methods. Blast transformation was induced in vivo with dinitrochlorobenzene (DNCB) and stimulated cells were labelled with tritiated thymidine. Random samples of cells were taken for light and electron microscopic morphometry. In comparison to the unstimulated lymphocyte, the stimulated cell increased in size and possessed a greater plasma membrane surface area. Increase in cell size was the result of increases in the volumes of all measured subcellular compartments both in the nucleus and the cytoplasm. Heterogeneity of the stimulated cell population precludes firm conclusions regarding the significance of all these ultrastructural changes, though alterations in cell surface are discussed in the context of known biochemical differences accompanying blastogenesis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00233538
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 27
    Electronic Resource
    Electronic Resource
    Hyphal growth ofHypomyces chlorinus Tul.: An autoradiographic study (1981)
    Springer
    Protoplasma 107 (1981), S. 221-233 
    Details
    ISSN: 1615-6102
    Keywords: Autoradiography ; Cell wall synthesis ; Chitin ; Hypomyces
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The incorporation of the chitin precursor N-acetyl-D-(1-3H) glucosamine byH. chlorinus has been studied by light and electron microscopic autoradiography. Light microscopic autoradiography showed that the incorporation occured preferentially at the hyphal apex. Autoradiograms from electron microscopy were quantitatively evaluated to determine the relative radioactivity incorporation between the cell wall and cytoplasm: this showed that (3H) incorporation took place mainly in the plasmalemma-wall complex. However a small amount of N-acetyl glucosamine can enter into the cytoplasmic space and is then transported by endomembranes (Golgi apparatus-vesicles) to the plasmalemma-cell wall interface before polymerization.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01276827
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 28
    Electronic Resource
    Electronic Resource
    Histochemical, cytochemical and autoradiographic studies on the rostellum ofHymenolepis diminuta (1981)
    Springer
    Parasitology research 64 (1981), S. 335-345 
    Details
    ISSN: 1432-1955
    Keywords: Hymenolepis diminuta ; Autoradiography ; Ultracytochemistry ; Histochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Histochemical studies on the rostellum ofHymenolepis diminuta revealed diastase-stable, protein/neutral carbohydrate-rich material localized in the rostellum tegument. The remainder of the rostellum, primarily composed of the glycogen-rich myocytons of the rostellum musculature, is protein-poor, but rich in diastase-labile, neutral and acidic carbohydrates. Ultrastructural cytochemical studies, using the periodic acid-thiocarbohydrazideosmium (PATCO) technique, indicated that the granules of the rostellar tegumental cytons and distal cytoplasm are carbohydrate-rich. Lipids are present in the rostellar myocytons but not in the tegumental cytons. Autoradiography using a pulse-labeling with [3H]leucine revealed an apical translocation of tegumental granules, but at a slower rate than had been reported for the strobilar tegument of the same organism. Neither [3H]galactose nor [3H]glucose were incorporated into the rostellar tegumental granules. The function of the secretory glycoprotein(s) produced in the rostellar tegument and its (their) possible role in the regulation of maturation and/or strobilization remain enigmatic.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00927380
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 29
    Electronic Resource
    Electronic Resource
    Observations on cytoplasmic transport along ovarian nutritive tubes of polyphagous coleopterans (1981)
    Springer
    Cell & tissue research 220 (1981), S. 153-161 
    Details
    ISSN: 1432-0878
    Keywords: Cytoplasmic transport ; Insect ovary ; Autoradiography ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The ovarioles of Coccinella and Tenebrio are shown to be telotrophic — a characteristic normally associated with hemipterans rather than coleopterans. They possess an anterior region of trophic cells and a chain of oocytes. The trophic cells are connected with the latter by a series of nutritive tubes, and autoradiography has shown that RNA is transported along the tubes to the oocytes. However, the system in these beetles differs markedly from that of hemipterans in that the nutritive tubes do not contain an extensive complement of aligned microtubules. The significance of this to both the mechanism and the selectivity of transport is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00209974
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 30
    Electronic Resource
    Electronic Resource
    Bilaterally asymmetrical uptake of [14C]2-deoxyglucose by the octavo-lateralis complexes in flatfish (1981)
    Springer
    Cell & tissue research 214 (1981), S. 659-662 
    Details
    ISSN: 1432-0878
    Keywords: Vestibular system ; Postural control ; Deoxyglucose method ; Autoradiography ; Flatfish
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary [14C]2-deoxyglucose uptake by neurons located in the octavo-lateralis complex of adult flatfish is asymmetrical on the two sides of the brain. It appears that the neuronal activity on the side oriented upward is higher than that on the side of the brain facing downward. This finding may be significant with respect to the mechanisms of metamorphosis of flatfish and may account for the peculiar fact that these animals swim on one body side during adult live.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00233505
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 31
    Electronic Resource
    Electronic Resource
    The effect of colchicine on the intracellular transport of 3H-fucose-labelled glycoproteins in the absorptive cells of cultured human small-intestinal tissue (1981)
    Springer
    Cell & tissue research 215 (1981), S. 1-12 
    Details
    ISSN: 1432-0878
    Keywords: Colchicine ; Human enterocyte ; Glycoprotein transport ; Autoradiography ; Biochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The effect of colchicine on the intracellular transport of 3H-fucose-labelled glycoproteins in the absorptive cells of cultured biopsy specimens of the human intestine was investigated by light- and electron-microscopical autoradiography and by biochemical methods. The results showed a decrease in the radioactivity of the cell coat on the microvilli and an increase in the Golgi apparatus and in the apical vesicles and tubules. This divergence is attributed to a colchicine-induced impairment of the normal transport of cell-coat glycoproteins from the Golgi apparatus, via the apical vesicles and tubules, to the apex of the cell. The radioactivity of the lysosome-like bodies in the absorptive cells cultured with colchicine also increased. This finding supports a crinophagic function of these organelles in the degradation of excess cell-coat material. The investigations were supported in part by the Foundation for Medical Research (FUNGO), which is subsidized by the Netherlands Organisation for the Advancement of Pure Research
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00236244
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 32
    Electronic Resource
    Electronic Resource
    Induction by progesterone of a sexual dimorphism of estrogen uptake by anterior pituitary cells in situ (1981)
    Springer
    Cell & tissue research 215 (1981), S. 75-86 
    Details
    ISSN: 1432-0878
    Keywords: Pituitary cells ; Estrogen ; Progesterone ; Autoradiography ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The effects of progesterone pretreatment on in vivo 3H-estrogen uptake by five anterior pituitary cell types was analyzed by means of a quantitative autoradiographic-immunocytochemical technique. Male and female rats castrated for 14 days show nuclear concentration of label in all five cell types one h after injection of 3H-estradiol, whether progesterone treated or not. The order of labeling intensity is gonadotropes ≧ lactotropes = somatotropes 〉 thyrotropes = corticotropes. Progesterone treatment induces a dramatic sexual dimorphism in estrogen uptake; it significantly increases 3H-estrogen uptake in all female cell types. In males, progesterone decreases uptake in gonadotropes while not altering uptake in other cell types.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00236250
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 33
    Electronic Resource
    Electronic Resource
    Monoamine precursor uptake in ganglia of a mollusc Anodonta cygnea L. (Bivalvia) (1981)
    Springer
    Cell & tissue research 219 (1981), S. 403-415 
    Details
    ISSN: 1432-0878
    Keywords: Monoamine precursors ; Ganglia ; Molluscs ; Autoradiography
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In vitro-uptake and localization of 3H-5-hydroxytryptophan and 3H-dihydroxyphenylalanine have been investigated by means of light microscopic autoradiography in the central nervous system of the freshwater mussel, Anodonta cygnea L. Accumulation of the labelled monoamine precursors could be observed both over neuronal perikarya and neuropil in the ganglia. 3H-5-hydroxytryptophan and 3H-dihydroxyphenylalanine were taken up by neurons characterized by a small size (10–20 μm), a polygonal form and an intense staining with toluidine blue. Labelled axon branches situated either in the neuropil or between nerve cells in the cortex exhibited only a moderate activity, frequently of a diffuse character. After pretreatment for 24 h with colchicine, silver grains were also seen over the neuronal perikarya and some axon profiles. This shows that local uptake of the monoamine precursors and, presumably, the synthesis of the monoamines takes place both at perikaryal and axonal/terminal level. The nerve cells accumulating the monoamine precursors contain a nucleus with rich chromatin content and a deeply invaginated nuclear envelope. Further ultrastructural characteristics of these neurons are their well-developed rough endoplasmic reticulum system, a great number of mitochondria and glycogen granules, and the presence of dense-cored vesicles of different types. All these features are responsible for the electron-dense appearance of the cytoplasm.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00210158
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 34
    Electronic Resource
    Electronic Resource
    In vitro binding of 125I-HCG to the rat ovary from birth to puberty (1981)
    Springer
    Cell & tissue research 214 (1981), S. 633-640 
    Details
    ISSN: 1432-0878
    Keywords: Rat ovary ; 125I-HCG binding ; Autoradiography ; Hormone receptors
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Neither homogenates nor frozen ovarian sections of rats aged 1, 5 and 7 days demonstrated specific binding of 125I-HCG. However, from day 10 to day 35, the homogenates could bind specifically, with an equilibrium association constant in the range of 0.9–2.7 × 1010 M−1. The binding capacity increased from 5.8 fmol/mg tissue at day 10 to 15.7 fmol/mg tissue at day 35. As revealed by autoradiography, the frozen ovarian sections from 10-day-old rats showed 125I-HCG binding localized over the interstitial and thecal tissues and, in the older age groups, also in the granulosa cells of follicles larger than 500 μm in diameter. These results indicate that LH/HCG receptors appear in the rat ovary at the beginning of the second postnatal week, and that the interstitial cells are the main site of action of LH before puberty.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00233502
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 35
    Electronic Resource
    Electronic Resource
    Localization of 3H-dihydrotestosterone in the pituitary gland of the rhesus monkey (1981)
    Springer
    Cell & tissue research 215 (1981), S. 499-504 
    Details
    ISSN: 1432-0878
    Keywords: Androgen receptors ; Pituitary gland ; Rhesus monkey ; Autoradiography ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The uptake and retention of radiolabelled dihydrotestosterone by the pituitary gland was examined in the rhesus monkey. Two animals were given an intravenous injection of 1.0μg/kg 3H-dihydrotestosterone (DHT) alone while one monkey received both the labelled androgen and 100μg/kg of unlabelled steroid. One and a half hours later, they were sacrificed. The pituitary glands were removed and processed for autoradiography and immunocytochemistry. Autoradiographic localization of DHT was discernible in the partes nervosa, intermedia and distalis, albeit the highest concentration of radiolabelled cells was noted in the pars distalis. Immunocytochemical staining with antibodies to rat PRL, human TSHβ and ovine LHβ revealed a population of steroid-concentrating cells that contained TSH and a second group that contained LH. None of the cells that reacted with the anti-PRL serum were radiolabelled.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00233526
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 36
    Electronic Resource
    Electronic Resource
    Evaluation of the effects of sensory denervation on osteoblasts by 3H-proline autoradiography (1981)
    Springer
    Cell & tissue research 217 (1981), S. 569-576 
    Details
    ISSN: 1432-0878
    Keywords: Autoradiography ; 3H-proline ; Osteoblasts ; Denervation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The inferior alveolar nerve was unilaterally resected in 30-day-old mice; other animals were unilaterally sham-operated. At 15, 30, 60, 90, or 150 days after surgery, the mice were injected with 2μCi of 3H-proline (sp. act. 1.0 Ci/mM) per g of body weight and killed 15, 30, or 60 min later. Autoradiographs were prepared from 5μm decalcified sagittal sections of mandibles and grain counts made over periosteal osteoblasts mesial to the first molar. In denervated mandibles, osteoblasts incorporated less isotope compared to controls with differences being maximal at the early intervals. These differences became attenuated with time, possibly due to an intrinsic compensatory mechanism, secondary to neurotrophic regulation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00219365
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 37
    Electronic Resource
    Electronic Resource
    Germinal centers and the B-cell system (1981)
    Springer
    Cell & tissue research 218 (1981), S. 59-73 
    Details
    ISSN: 1432-0878
    Keywords: Germinal centers ; B-cell differentiation ; Lymphocyte migration ; Autoradiography ; Lymphoid tissue ; Appendix (rabbit)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The migration pattern of germinal center cells of the rabbit appendix was studied and compared with that of appendix dome cells, spleen cells, thymus cells and thoracic duct lymphocytes. To discriminate T-and B-cell migration pathways, normal or T-cell-depleted rabbits were used as donors. Cell suspensions were labeled in vitro with 3H-leucine followed by intravenous transfer. The migration of labeled cells in lymphoid organs was studied using autoradiography, particular attention being paid to the spleen of the recipient. B-cells from the appendix dome, spleen and thoracic-duct lymph migrate to primary follicles or the corona of secondary follicles via thymus-dependent areas of peripheral lymphoid organs. In contrast, a B-cell subpopulation from the germinal centers of the appendix migrates to the center of splenic primary follicles and into germinal centers. The migration of germinal center cells to splenic follicle centers is not enhanced by specific antigens. The migration properties of B-cells, possibly changing during differentiation, may be instrumental in the two types of immune reactions, i.e., plasma-cell reaction and germinal-center reaction.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00210091
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 38
    Electronic Resource
    Electronic Resource
    Localization of 3H-estradiol in the uterus of pregnant and non-pregnant armadillos (1981)
    Springer
    Cell & tissue research 220 (1981), S. 773-780 
    Details
    ISSN: 1432-0878
    Keywords: Estrogen receptors ; Uterus ; Armadillo ; Autoradiography
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The uptake and retention of radiolabeled estradiol by the uterus was examined in the armadillo. One pregnant and two non-pregnant armadillos were treated with 1.4 μg/kg body weight of 3H-estradiol (E2) by injection into the left ventricle, and one non-pregnant animal was injected with both the labeled hormone and 140 μg/kg body weight of unlabeled E2. One and a half hour after injection, the animals were sacrificed and the uteri were removed and processed for autoradiography. In the non-pregnant animals, nuclear localization was observed in the interstitial cells and glandular epithelium of the endometrium and the connective tissue cells and smooth muscle of the myometrium. Additionally, there was a gradation of uptake in the epithelial cells of the endometrium in that the glandular cells of the basal region were heavily labeled, while those cells in the sinusoidal, and luminal regions contained successively less label. The luminal cells were poorly labeled. In the pregnant female, the smooth muscle and glandular cells hypertrophied and their nuclei contained less label than was observed in the non-pregnant animals. The arteries of the myometrium were more easily distinguished in the pregnant animals and the nuclei of the endothelial cells and smooth muscle were more consistently labeled than those of the non-pregnant armadillos.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00210460
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 39
    Electronic Resource
    Electronic Resource
    Autoradiographic localization of 3H-GABA uptake in the thyroid gland of the rat (1981)
    Springer
    Cell & tissue research 220 (1981), S. 873-879 
    Details
    ISSN: 1432-0878
    Keywords: γ-Aminobutyric acid (GABA) ; Autoradiography ; Thyroid gland ; Transport ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The uptake of γ-aminobutyric acid (GABA) in the thyroid gland of the rat was studied autoradiographically following in vitro incubation. High-affinity GABA uptake was localized in follicle cells, whereas C cells (parafollicular cells) in general did not accumulate GABA by high-affinity transport. The follicle cells were also the main sites of low-affinity GABA uptake. Additionally, some nerve fibres were found to accumulate GABA. The predominant localization of GABA uptake in follicle cells is discussed in view of a presumed role of GABA in thyroid function.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00210468
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 40
    Electronic Resource
    Electronic Resource
    Autoradiographic studies with 3H 1,25 (OH)2 vitamin D3 and 3H 25 (OH) vitamin D3 in rat parathyroid glands (1981)
    Springer
    Cell & tissue research 221 (1981), S. 333-338 
    Details
    ISSN: 1432-0878
    Keywords: 1,25(OH)2 ; Vitamin D3 ; Receptors ; Parathyroid glands ; Autoradiography
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary After injection of radiolabeled 1,25 (OH)2 vitamin D3, nuclear concentration of radioactivity is observed in parenchymal cells of the parathyroid gland in pregnant, adult male, and 10-day male neonatal rats. In competition studies with unlabeled 1,25 (OH)2 vitamin D3, but not with 25 (OH) vitamin D3, nuclear uptake is prevented. Experiments with 3H 25 (OH) vitamin D3, in contrast to 3H 1,25 (OH)2 vitamin D3, do not show nuclear concentration in cells of the parathyroid. The results of the autoradiographic studies suggest the presence of receptors for a direct effect of 1,25 (OH)2 vitamin D3 on the parathyroid gland for modulation of parathyroid hormone secretion.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00216737
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 41
    Electronic Resource
    Electronic Resource
    Functions of digestive diverticula in marine invertebrates (1981)
    Springer
    Cell & tissue research 219 (1981), S. 185-195 
    Details
    ISSN: 1432-0878
    Keywords: Glucose ; Pyloric gland ; Ascidian ; Autoradiography
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The movement of labelled glucose along the digestive tract of two ascidian species, Sidnyum argus and Dendrodoa grossularia is compared. In Dendrodoa grossularia, glucose absorption takes place in all parts of the digestive tract except the oesophagus. Labelled glucose passes through the digestive tract in less than 12 h, and it accumulates in the pyloric ampullae in less than 3 days. In this species, the pyloric gland has more capacity to stock glucidic compounds than other tissues. Under normal feeding conditions, after five days, there is no transfer of labelled glucose to other tissues. Whatever the substances given to Sidnyum argus, its pyloric gland never accumulates labelled products.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00210027
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 42
    Electronic Resource
    Electronic Resource
    Aspects of cellular proliferation during follicular atresia in the dog ovary (1981)
    Springer
    Cell & tissue research 219 (1981), S. 173-183 
    Details
    ISSN: 1432-0878
    Keywords: Ovary (dog) ; Atresia ; Thymidine ; Autoradiography
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Autoradiography after pulse labelling with [3H] thymidine was applied to investigate the proliferation processes in the granulosa and theca related to follicular atresia of the dog ovary during metestrus. The number of proliferating cells depends on the follicle type and its atretic stage. There is less proliferation in smaller secondary follicles than either in larger ones or tertiary follicles. While in early atresia tertiary follicles show the highest labelling indices, in advanced atresia the larger secondary follicles are those with the highest values. For each follicle type a decline in the labelling indices can be observed from early to terminal atresia. Tertiary follicles show a precipitous decrease in the labelling index between early and advanced atresia. There is a continuous gradient of proliferation from the center of the follicle over the peripheral granulosa to the theca. In tertiary follicles, an inverse correlation between labelling and necrosis of granulosa cells can be observed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00210026
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 43
    Electronic Resource
    Electronic Resource
    p-Chlorophenylalanine-induced proliferation of the seminal vesicle epithelium (1981)
    Springer
    Cell & tissue research 219 (1981), S. 159-172 
    Details
    ISSN: 1432-0878
    Keywords: Seminal vesicles ; Proliferation ; Autoradiography ; Biochemistry ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Intraperitoneal injection of p-chlorophenylalanine (pCPA) methylester (100 mg/kg body weight) results in an activation of the lysosomal system of the secretory cells in the rat seminal vesicle and an elevation of the activities of lysosomal enzymes within 15 min following the injection. Large autophagic vacuoles are formed, sequestering rough endoplasmic reticulum and part of the Golgi apparatus within 2 h. Shortly after the activation of the lysosomal system an elevation of both DNA and protein synthesis is measured biochemically. 6 h subsequent to the injection a wave of mitoses of the secretory cells begins, reaching a maximum 6 h later and then declining within 3 h. About 12 h following the injection a second rise in lysosomal activity begins, declining within 24 h. The entire sequence of lysosomal and proliferative activities is inhibited in antiandrogen-pretreated rats. Deduced from these findings the following hypothesis of growth regulation of the accessory sex glands is advanced: enhanced loss of intracellular material during autophagocytosis diminishes the intracellular concentration of a substance curtailing cell division below its effective threshold resulting in division of the secretory cells. The prerequisites of this mechanism are (i) a sufficient distributive capacity of the stroma for hormones (androgens) and metabolic precursors, and (ii) sufficient capacity of the basal cells for transporting the precursors to the secretory cells. Sloughing of the secretory cells separates them from these auxiliary structures (stroma and basal cells) and enables the basal cells to divide.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00210025
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...