ISSN:
1476-5535
Schlagwort(e):
Keywords: kojic acid; Aspergillus flavus; fermentation kinetics; resuspended cell system; C/N ratio
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Biologie
,
Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
Notizen:
Kinetics of kojic acid fermentation by Aspergillus flavus Link 44-1 using various sources of carbon [glucose, xylose, sucrose, starch, maltose, lactose or fructose] and nitrogen [NH4Cl, (NH4)2S2O8, (NH4)2NO3, yeast extract or peptone] were analyzed using models based on logistic and Luedeking–Piret equations. The highest kojic acid production (39.90 g l−1) in submerged batch fermentation was obtained when 100 g l−1 glucose was used as a carbon source. Organic nitrogen sources such as peptone and yeast extract were favorable for kojic acid production as compared to inorganic nitrogen sources. Yeast extract at 5 g l−1 was optimal. The optimal carbon to nitrogen (C/N) ratio for kojic acid fermentation was 93.3. In a resuspended cell system, the rate of glucose conversion to kojic acid by cell-bound enzymes increased with increasing glucose concentration up to 70 g l−1, suggesting that the reaction followed the Michaelis–Menten enzyme kinetic model. The value of K m and V max for the reaction was 18.47 g l−1 glucose and 0.154 g l−1 h−1, respectively. Journal of Industrial Microbiology & Biotechnology (2000) 25, 20–24.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1038/sj.jim.7000017
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