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  • Articles  (4)
  • sperm  (4)
  • Wiley-Blackwell  (4)
  • American Association for the Advancement of Science
  • American Physical Society
  • Annual Reviews
  • Wiley
  • 1980-1984  (4)
  • 1935-1939
  • 1980  (4)
  • Biology  (4)
Collection
  • Articles  (4)
Source
Keywords
Publisher
  • Wiley-Blackwell  (4)
  • American Association for the Advancement of Science
  • American Physical Society
  • Annual Reviews
  • Wiley
Years
  • 1980-1984  (4)
  • 1935-1939
Year
Topic
  • Biology  (4)
  • 1
    Electronic Resource
    Electronic Resource
    Quantitation of avian spermatozoan motility: Neurochemical regulation (1980)
    New York, NY : Wiley-Blackwell
    Gamete Research 3 (1980), S. 17-24 
    Details
    ISSN: 0148-7280
    Keywords: sperm ; motility ; neurochemical ; paraoxon ; acetylcholine ; cholinesterase ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: The hypothesis that motility of avian sperm is regulated by acetylcholine was examined by treating rooster (Gallus domesticus) sperm with choline analogs and paraoxon, an inhibitor of colonesterases. Acetylcholine chloride (AChCl) was most effective, acetylthiocholine iodide and butyrylthiocholine iodide were less effective, and choline chloride was ineffective in stimulating sperm motility. Histochemical localization of cholinesterase activity with the electron microscope showed enzyme activity to be associated with membranes of the head and within fibrillar components of the tail. Increasing concentrations of paraoxon decreased cholinesterase activity and increased sperm motility. The data provide evidence that the motility of avian sperm, like that of mammal and sea urchins, may be regulated in part by a system with similarities to the cholinergic neurotransmitter system.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrd.1120030104
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  • 2
    Electronic Resource
    Electronic Resource
    Artificial fertilization of gametes from the South African clawed frog, Xenopus laevis (1980)
    New York, NY : Wiley-Blackwell
    Gamete Research 3 (1980), S. 45-57 
    Details
    ISSN: 0148-7280
    Keywords: clawed frog ; egg ; fertilization ; jelly coat ; motility ; sperm ; Xenopus laevis ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: A reproducible and effective method for fertilization eggs of Xenopus laevis was developed based of systematic manipulation of environmental factors. The effects of varying concentrations of individual components of a fertilization medium were tested by measuring jelly swelling, sperm motility, and sperm longevity. Results were used to develop an improved medium for fertilization, consisting of 41.25 mM NaCl, 1.25 mM KCl, 0.25 mM CaCl2, 0.0625 mM MgCl2, 0.5 mM Na2HPO4, 2.5 mM HEPES, 1.9 mM NaOH, final pH(2°) 7.8.
    Additional Material: 12 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrd.1120030106
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  • 3
    Electronic Resource
    Electronic Resource
    Fragmentation of condensed chromatin in nuclei of a plant sperm, pteridium aquilinum (L.) Kuhn (1980)
    New York, NY : Wiley-Blackwell
    Gamete Research 3 (1980), S. 291-298 
    Details
    ISSN: 0148-7280
    Keywords: condensed chromatin ; sperm ; Pteridium ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Nuclei of pteridium sperm have been dispersed by turbulence in natural or slightly alkaline buffer after stripping off the cytoplasm with nonionic detergent. The nuclei tended to break up into fragments arranged in a linear order. These fragments fluoresced brightly with acridine orange as did intact nuclei. Grounds are given for identifying the smaller fragments with chromosomes. It is proposed that the sperm nucleus of British Pteridium, possibly an autotetrapolid, consists of a sequence of paired homologues.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrd.1120030311
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  • 4
    Electronic Resource
    Electronic Resource
    Rabbit testis proacrosin: Immunological similarities between testis, sperm proacrosins and the initially formed testis acrosin (1980)
    New York, NY : Wiley-Blackwell
    Gamete Research 3 (1980), S. 241-246 
    Details
    ISSN: 0148-7280
    Keywords: testis ; sperm ; proacrosin ; acrosin ; immunological ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Anti-rabbit proacrosin IgG was prepared from goat serum following immunization with a homogeneous preparation of rabbit testis proacrosin. The “auto-activation” products of purified testis proacrosin were separated into 68,000 and 34,000 molecular weight (mol wt) acrosins by Sephadex G-100 column chromatography. Immunodiffusion analysis of testis and epididymal sperm proacrosins and acrosins on agarose gel against goat anti-rabbit testis proacrosin showed immunological identity between rabbit testis and sperm proacrosins and the initial testis acrosin (mol wt 68,000). However, the 34,000 mol wt form of testis acrosin showed weaker reaction with the antibody and only partial identity with the proacrosin and the 68,000 mol wt form of acrosin. These results suggest that there is no major structural difference between testis and sperm proacrosins and between proacrosin and the 68,000 mol wt acrosin, but such a structual change occurs when the 34,000 mol wt acrosin is formed.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrd.1120030306
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