ALBERT

Notes: A breccia vein sampled from a shear zone in greenschist facies metapelites at Mount Isa, Queensland, Australia, shows a systematic variation in vein geometry that is related to the geometry of folding and faulting within the sample. Calcite vein-fill is coarse grained and equigranular, suggesting precipitation in a fluid-filled space. Partially folded veins suggest that veining occurred during folding and faulting. The breccia vein contains a central zone in which dilation has occurred simultaneously in all directions in the plane of section, implying that this was a zone of high fluid pressure and nearly isostatic differential stress during folding and faulting. From these observations, it can be inferred that the breccia vein was a zone of high permeability and a likely fluid channel during deformation. This hypothesis was tested by stable isotope analysis of veins and host rocks. The calcite veins have δ13C values of -11.1 ± 0.1% and δ18O values of 6-10%o, whereas the host metapelite has δ13C values of -10.62 and -10.11% and δ18O values of 14-15%o. These values are consistent with an igneous-derived, H2O-dominated fluid that exchanged little oxygen with the host rocks, but derived much of its carbon from the wall rock. The isotopic disequilibrium between the veins and the wall rock confirms that the fluid was externally derived, and that the breccia vein acted as a channel for large-volume fluid flow within the shear zone.

Notes: This paper describes the progressive metamorphism and deformation of a series of metasediments, Le Conquet Schists and their higher grade equivalents, which occur as tectonically emplaced screens within a sequence of foliated gneisses, the Gneiss de Brest and Gneiss de Lesneven. The sequence exhibits a steep south to north increase in metamorphic grade from garnet-staurolite schist to sillimanite gneiss and sillimanite-K-feldspar migmatite. The relationship of mineral growth to foliation development has been established for individual screens. At least five phases of deformation (D1-D5) are preserved. Analysis of porphyroblast inclusion trails is used to demonstrate sequential mineral growth during the successive development of orthogonal foliations S1-S4. Porphyroblasts continued to grow during the subsequent development of C-S mylonite fabrics and extensional crenulation cleavages which are genetically related to a series of high-strain zones (D5). Mineral assemblages, phase relations and mineral chemistry are consistent with porphyroblast growth being the result of continuous reactions. Microstructure-porphyroblast relations are used to show that although mineral growth proceeded during continuous reactions, these only operated episodically. Phase relations, mineral chemistry and P-T estimates are used to constrain P-T trajectories and these are linked to the deformation histories within individual screens. A comparison between the resulting pressure-temperature-deformation paths is used to demonstrate that the metamorphic peak occurred progressively later and at successively lower pressures with increasing metamorphic grade. It is suggested that the early evolution of the belt is the result of crustal thickening by overthrusting. The subsequent history is one of progressive heating and unroofing of the higher grade rocks in a dextral strike-slip transtensional shear zone.

Notes: Porphyroblast inclusion trails have the potential to provide critical information about tectonometamorphic events. Recently, however, traditional interpretations of inclusion trails have been called into question by the suggestions that porphyroblasts do not rotate during non-coaxial deformation and that apparent spiral inclusion trails can be generated in coaxial deformation. We present a new computer model that simulates inclusion trail development. Model results suggest: (1) that the extent of porphyroblast rotation is controlled by conditions at the porphyroblast-matrix boundary; (2) that curved inclusion trails may develop in unrotated porphyroblasts; (3) that classic “snowball” inclusion trails are most simply explained by rotational growth histories; and (4) that some of the observations used to support the view that porphyroblasts do not rotate (e.g. weakly sigmoidal inclusion trails, apparent truncations of inclusion trails) can be accounted for by variations in the growth rate of rotating porphyroblasts.

Notes: The feasibility of measuring soil salinity with electromagnetic induction (EM) for determining riparian restoration potential was investigated on a 28-hectare plot at the Bosque del Apache National Wildlife Refuge in central New Mexico. The plot was cleared of exotic Tamarix chinensis (saltcedar), surveyed and gridded into 1370.2 hectare sections. Soil samples and EM measurements were taken at each section. We compared laboratory-determined ECe values from the soil samples with ECa values calculated from the EM measurements using a model developed by Rhoades et al. (1990). Direct comparison of ECe values determined from the two methods yields a low correlation due to sample-size differences but the calculated ECa was able to accurately predict whether the measured ECe would lie above or below some threshold value. An assessment of general site suitability for riparian restoration with electromagnetic induction has proven to be a rapid, accurate, and cost-effective alternative to intensive soil sampling.

Notes: Extensive areas of the tropics have been converted into pasture for cattle ranching. Frequently, abandoned pasture does not revert to forest. The goal of this project was to identify barriers to lowland moist forest regeneration in highly degraded grasslands in the Sierra Nevada de Santa Marta, Colombia. The barriers we considered were seed source, seed predation, competition with grasses, microclimate and soil limitations on plant growth, and fire. Seed dispersal into the grasslands is limited to within 10 meters of forest fragments, but this barrier can be overcome by sowing seeds and planting seedlings and by establishing perches to attract dispersers. In these degraded grasslands, seed predation was lower than in the adjacent forest patches, and there was no evidence that grasses inhibited the establishment of woody species. The most important barrier was the severe degradation of the soils. In much of the area, the A and B horizons have been eroded away, leaving saprolite at the soil surface. Seedlings of two fast-growing pioneer species, Ochroma pyramidale and Cochlospermum vitifolium, grew to a maximum height of only 2.5 and 12 cm, respectively, during the first eight months. The slow plant growth in the degraded grassland soils compared to forest soils was associated with lower levels of cation-exchange capacity, calcium, magnesium, and potassium. Even if these barriers could be overcome, the frequent and extensive use of fire in the region must be controlled to avoid killing established woody plants.

Notes: Biological resources can be more usefully incorporated into many aspects of restoration ecology. During the planning and design stage, the wide genotypic variation in natural plant populations must be recognized and exploited. This will ensure that genotypes used on a site are best adapted to local conditions and have a greater probability of survivorship than arbitrarily chosen material. Also, certain unusual genotypes can be located using the principles of evolutionary ecology and can be installed in areas with extreme conditions, such as soils contaminated with heavy metals, in areas where rapid colonizing ability (high seed set and/or clonal growth) is particularly advantageous, or where soils are of poor quality. Similarly, where high herbivore pressure is a threat to restoration, genotypes that are well defended, chemically or mechanically, against animal enemies should be selected to initiate the restoration process. The nursery industry can be encouraged to supply an ecologically wider selection of material for restoration, originating from local biological reserves and natural habitats. During the management phase of a restoration, local natural habitats are critical as reservoirs of biological control agents, seed sources for plant species, and members of higher trophic levels and additional plant species needed during succession. Mutualists such as pollinators, seed dispersers, and mycorrhizal fungi are vital to the success of a restoration project, and these must invade from nearby natural habitats or must be deliberately introduced. During the evaluation phase of restoration, local natural areas should be used as templates of community composition and structure from which one measures success. A functioning restoration project will interact biologically with surrounding areas, the exchange of species and genes being particularly important. Analysis of the microbial and invertebrate communities that have invaded the installed plant community may be useful and accurate determinants of ecological function. For these latter stages of the restoration process, the value of preserving local habitat remnants is high and complements their usefulness as a source of ecologically precise material for installation.

Notes: Community classification has emerged from ecology textbooks into the arena of environmental impact evaluation and mitigation. This development is especially apparent in southern California, where the fate of a community called coastal sage scrub is being decided. Regional plans for development, mitigation, preservation, and restoration are being formulated that will permanently affect the natural landscape. This paper demonstrates the potential for the name of a plant community to affect tradeoffs in planning processes that are intended to offset removal of habitat by development. The discussion focuses on two types of classification systems. One system is hierarchical, with established nomenclatorial rules that allow natural variation to refine the community definitions. Names of plant associations directly reflect the dominant species in the association, as in Artemisia californica—Eriogonum fasciculatum (California sagebrush—California buckwheat). The second system is nonhierarchical, in which observations of natural landscapes are fit into established definitions of community types, such as Diegan coastal sage scrub. Advantages and disadvantages of both types of systems are discussed and illustrated by two examples of problems in classifying coastal sage scrub. The discussion concludes with the point that the name of a community has the potential to significantly affect the extent to which equal tradeoffs between community types are actually achieved in mitigation and restoration efforts.

Notes: This article contributes to the worldwide trend of using microbiological methods for bioindication of the influence of abiotic, biotic, and anthropogenic factors on the soil environment, especially on the plough layer of soils. It compares two approaches to assess microbial biomass-C: (1) chemical treatment of soil samples by CHCl3 vapor, followed by extraction, and (2) heat treatment in the range of 62–65°C for 24 hours, followed by extraction. It recommends the analysis of different forms of organic carbon, the total carbon of soil organic mass, and microbial biomass-C and other forms of organic carbon extractable with 0.5 M K2SO4 and 0.5 M (NH4)2SO4 respectively. It presents a simple mathematical model suitable for assessment of microbial recuperation of the topsoil (0–15 cm) after restoration. The relation between the measured values and the values calculated with the model expressed as percentages expresses the quality of restoration achieved and the stage of disturbance of the living microbial part of the topsoil. It indicates not only the ecological impact but also the deficit and stress situations, to which the living microbial part of the topsoil is exposed in the long term.

Notes: Books reviewed in this article: Ecological Principles of Nature Conservation. L. Hansson, editor Saltmarsh Ecology. P. Adam

Notes: Within an urban park in southern California, the relationship between the structure and floristics of vegetation and the distribution, abundance, and behavior of wildlife was studied in relatively undisturbed areas (San Luis Rey) and in contiguous areas (Guajome Park) in need of restoration. These data were used to develop recommendations for the enhancement of native animal species in the park. The abundance of amphibians and reptiles was highest in native upland scrub and willow (Salix)-riparian vegetation types, and lowest in dry, disturbed sites. Western fence lizards (Sceloporus occidentalis) were the most abundant reptile throughout both study areas. Overall, bullfrogs (Rana catesbiana), an exotic species, were the dominant amphibians; the native Pacific treefrog (Hyla regilla) was rare throughout. At both study areas, the small mammal community was dominated by western harvest mice (Reithrodontomys megalotis) and deer mice (Peromyscus maniculatus) and, to a lesser extent, by brush mice (P. boylii) and exotic house mice (Mus musculus). Negative correlations in abundance existed between house mice and harvest mice, and between house mice abundance and overall small mammal abundance. In riparian sites, cottonwood (Populus fremontii) and various height classes of willow were the dominant factors in the majority of bird abundance–habitat‘correlations and where foraging activities were concentrated. Recommendations for enhancing native animal species include reduction of marsh sedimentation, removal of feral species, and development of connections between the park and nearby natural areas. A corridor of native riparian vegetation (primarily cottonwood-willow) should be developed to replace the existing agricultural fields, thereby linking Guajome with the San Luis Rey River.

Notes: Eight individual rock iguanas (Iguana pinguis) from Anegada Island were relocated to Guana Island by Lazell, 1984–1987, in order to establish a second population reservoir for this endangered species. The species may have originally occupied the entire Puerto Rico Bank. The relocation has been successful and, in the area currently providing the best habitat, we estimate a density of 9 or 12 animals of various age classes per 19 ha. The optimal area contains a sheep exclosure with relatively dense understory vegetation and numerous exotic as well as native species of plants. Iguana activity is concentrated on east facing slopes and ridge-tops that get morning sun. Outside the exclosure most edible ground cover and shrubs have been eaten by sheep, leaving toxic or noxious species, such as Croton or Lantana, in the understory where I. pinguis adults generally forage. Removal of sheep may be critical to continued population growth of these reptiles. Views on relocation or repatriation of other endangered Antillean Iguana species are advanced, with some ideas on minimum viable population sizes and a possible explanation for the extirpation of I. pinguis from much of its former range.

Notes: Books reviewed in this article: Functions of Nature Evaluation of Nature in Environmental Planning, Management and Decision Making. Rudolf S. de Groot Landscape Restorastion Handbook D. Harker, S. Evans, M. Evans, and K. Harker

Notes: While the economic approach to the politics of regulation emphasizes the importance of organized economic interests in shaping policies, political institutions in which regulatory agencies are embedded may also have significant effects. By including both economic influences and characteristics of political institutions in a model of price setting by state regulators, this paper demonstrates that both shape regulatory behavior in the telecommunications industry. Whether commissioners are elected or appointed, whether they face confirmation by a legislature, and whether a single party controls both executive and legislative branches of state governments influence the level of prices charged for basic services.

Notes: We investigate the choice of compensation scheme by firms. Our basic model shows that the unique equilibrium choice for profit maximizing duopsonists in a labor market is for one firm to offer a wage rate and for the other to offer a piece rate. This result arises because the firms recognize that, by offering different compensation schemes, they induce self-selection among workers, which thereby decreases the intensity of competition in the labor market. We find this asymmetry to be robust to allowing for firing, free entry, and a class of more general compensation schemes. When we broaden our model to permit firms to be differentiated in the eyes of workers (either geographically or by “other working conditions,” e.g.), we find that our results are preserved when differentiation is low, but that both firms choose to offer a piece rate when differentiation is high.

Notes: In the present study I identify an inherent characteristic of health care markets that may lead to excessive investment by hospitals even when compensated according to a prospective reimbursement rule. It is demonstrated that the stochastic nature of the demand for medical services combined with the lumpiness of investment decisions may give rise to excessive investment when multiple hospitals select independently their levels of capacities. The source for the excessive incentives to invest is the difficulty of one hospital to internalize properly the externality generated by its investment decisions. Such an externality arises because when one hospital expands its capacity, it is more likely to be able to serve not only patients residing in its region but patients residing in neighboring regions as well.

Notes: What is the nature of the industrial organization of the market for physician services? Is the market “competitive?” Are there pareto-relevant market failures, such that there is room for welfare-improving policies? Economists have devoted a great deal of attention to this market, but it remains relatively poorly understood. The key features of this market are that the product being sold is a professional service, and the pervasive presence of insurance for consumers. A professional service is inherently heterogeneous, nonretradable, and subject to an asymmetry of information between buyers and sellers. These characteristics are what bestow market power on sellers, further strengthened by the fact that consumers face only a small fraction of the price of any service due to insurance. This paper considers the implications of these characteristics for agency relationships between patients and physicians, and insurers (both private and public) and physicians. Agency relationships within physician firms are also considered. Both theoretical and empirical modeling of contracting between insurers and physicians and of the joint agency problems between patient and physician and insurer and physician are recommended as areas for future research. Because failures in this market are seen to derive largely from the structure of information, the potential gains from government intervention may be sharply circumscribed. Nonetheless, careful consideration of the competitive implications of contracting between physicians, insurers, and other health care providers is an important area for antitrust policy.

Notes: Books reviewed in this article: Economics, Organization and Management, by Paul Milgrom and John Roberts

Notes: The publication of the passive learning model of Jovanovic and subsequently the active exploration model of Ericson and Pakes led to a resurgence in research on firm growth and survival. Empirical work measuring the relationship of growth and survival relative to size and age uncovered a number of strong regularities, consistent with the new theoretical models and also with a rather broad class of models of which the above represent special cases. But recent theoretical work has shown that when the learning models are applied to the special case of the labor managed firm, most of the sharp empirical predictions derived for profit maximization are rendered indeterminate. Given the impact of ownership structure on the theoretical results of the learning models, we use a dataset of French producer cooperatives to determine whether firms of different ownership structures actually have substantially different growth and survival patterns. We find that French cooperatives survive and grow in very much the same way as has been found for U.S. firms, suggesting that the empirical regularities previously uncovered hold for a much broader class of firms. We are left to conclude that the underlying empirical growth and survival relationships continue to hold for firms with sharply differing ownership structures, and the theoretical models are attempting to explain these broad-based relationships by means of overly specific modeling.

Notes: This paper uses origin-destination data to define geographic local hospital markets in large metropolitan statistical areas (MSAs). Results support past findings of service rather than price competition, with negative-cost Herfandahl-Hirschman indexes relationships at the market level (and sub-MSA level) and with profit margins negatively related to hospital market competition. The effects of total (direct plus indirect) market competition are unchanged between 1983 and 1988, precisely estimated, robust to estimation techniques, and unaffected by whether the number of direct competitors is included as an explanatory variable.

Notes: Users of photogrammetric data have often been puzzled by conflicting advice given by companies regarding achievable height accuracies which could be expected from varying scales of aerial photography. The author chairs the Photogrammetry Group which is convened under the auspices of the Council of the UK Land and Hydrographic Survey Association (TSA). The first aim of this Group was to establish some common ground among TSA member companies with regard to achievable heighting accuracies. A series of tests was carried out on the most commonly used photographic scales and guidelines were published to clients based on these tests. 〈section xml:id="abs1-2"〉〈title type="main"〉Résumé Les utilisateurs des données photogrammétriques ont été souvent déconcertés par les avis contraires émanant des entreprises concernant les précisions altimétriques accessibles à partir de diverses échelles des photographies aériennes. Dans ce contexte, le Conseil de ľ‘Association britannique des levées terrestres et hydrographiques (TSA) a mis sur pied un Groupe “Photogrammétrie” et en a confié la présidence àľauteur. Le premier objectif de ce Groupe a consistéàétablir des bases communes aux entreprises membres de la TSA, en ce qui concerne les précisions altimétriques réalisables. On a effectué un ensemble ď essais sur les échelles des photographies aériennes les plus communément utilisées et ľ on a publicé, à partir de ces essais un opuscule destinéà guider les clients. 〈section xml:id="abs1-3"〉〈title type="main"〉Zusammenfassung Die Nutzer photogrammetrischer Daten werden oft durch widerspriich-liche Ratschläge der Firmen in bezug auf die bei verschiedenen Bildmaβstäben erreichbare Höhengenauigkeit verwirrt. Der Autor leitet den photogrammetrischen Ausschuβ, der unter Aufsicht des Council des UK Land and Hydrographic Survey Association (TSA) einberufen wurde. Erstes Ziel dieses Gremiums war es, eine gemeinsame Grundlage unter den TSA-Mitgliedsgesellschaften im Hinblick auf erreichbare Höhengenauigkeit zu schaffen. Es wurde eine Reihe von Versuchen zu den am meisten verwendeten Bildmaβstäben durchgeführt, undes wurden daraufberuhende Richtlinien für Kunden veröffentlicht.

Notes: Cartography andphotogrammetry have always been strongly linked and this paper emphasises a view of cartography as a process of spatial data representation, rather than as a method of creating a product, a map. Of course, the definition of cartography must change with developments in its practice. An outline of the historical background to the subject is presented, followed by a consideration of the relationship between the cartographer and the user of the product. The basic problems involved in the transfer of information from the complex real world to a generalized cartographic product are analysed and technological aspects of the discipline are described. Contemporary applications of cartography include hazard mapping, links with GIS, tactile products and electronic hydrographic representation, all of which represent customer driven progress. It is concluded that the future of cartography is, overall, dependent on a graphical representation of high quality being supplied to the end user, rather than on the methods used to attain this objective. 〈section xml:id="abs1-2"〉〈title type="main"〉Résumé La cartographie et la photogramétrie ont toujours été liées étroitement ľune àľautre, et ľ‘on souligne dans cet article ľ‘idée que la cartographie est un systéme de représentation des données à trois dimensions, plutôt qu‘un ensemble de procédés de fabrication ďun produit, la carte. II est évident que la définition de la cartographie doit changer au fur et à mesure que des évolutions apparaissent dans sa pratique. Aprés un aperçu du contexte historique de ce thème, on examine les relations qui se développent entre le cartographe et ľutilisateur du produit. On analyse les problèmes fondamentaux du transfert ďinformations du monde réel, complexe, à un produit cartographique géneralisé et ľ‘on décrit la technologie qu'utilise cette discipline. Les applications récentes de la cartographie concernent la cartographie des risques naturels, les relations avec les SIG, les cartes qui se lisent par le toucher, et la représentation electronique de ľ‘hydrographic produits qui tous sont issus de cheminements pilotés en aval, par le client. On en déduit que la cartographie est, àľ'avenir, par dessus tout fonction ďune represséntation graphique de haute qualityé fournie à un utilisateur final, plutôt que de processus utilisé pour parvenir à cet objectif.〈section xml:id="abs1-3"〉〈title type="main"〉Zusammenfassung Kartographie und Photogrammetrie waren immer eng verbunden, und dieser Beitrag betont starker eine Auffassung von der Kartographie als einem Prozeβ der Darstellung von Raumdaten als ein Verfahren zur Herstellung eines Erzeugnisses, der Karte. Natürlich muβ sich die Definition der Kartographie bei Entwicklungen in ihrer Praxis ändern. Es erfolgt eine Skizzierung des historischen Hintergrundes des Gegenstands, gefolgt von Betrachtungen zur Beziehung zwischen Kartographen und dem Nutzer des Erzeugnisses. Die mit der Übertragung von Informationen aus der komplexen realen Welt in ein generalisiertes kartographisches System verbundenen grundlegenden Probleme werden analysiert, und es werden technologische Aspekte der Disziplin beschrieben. Zeitgenössische Anwendungen der Kartographie schlieβen die Kartierung von Katastrophen, Verbindungen zu GPS, gefühlsmäβge Erzeugnisse und elektronische hydrographische Darstellungen ein, die alle den vom Nutzer beeinflufiten Fortschritt darstellen. Es wird geschluffolgert, daft die Zukunft der Kartographie vor allem von der graphischen Darstellung hoher Qualität, die dem Endnutzer geliefert wird, abhängig ist undnicht so sehr von den Methoden, me dieses Ziel erreicht wird.

Notes: Since its foundation in 1982, the Institut Cartogràfic de Catalunya has been dealing with digital images. Though digital images and techniques were initially confined to remote sensing, they have been gradually transferred to and combined with photogrammetry. In addition to the development of remote sensing and digital orthophotography systems, this expertise has facilitated the introduction of commercially available digital photogrammetric systems (scanning and stereocompilation) which are now in full operation. The paper describes the experience in scanning, orthophotography production and stereocompilation, draws some conclusions and gives an insight of the future of digital photogrammetry. 〈section xml:id="abs1-2"〉〈title type="main"〉Résumé L‘Institut Cartographique de Catalogue s‘est intéressé au traitement des images numériques depuis le moment où il a été crée, en 1982. Bien qu'au début les images numériques et leur technologie aient été réservées à la télédétection, elles se sont trouvées peu à peu transférées et intégrées à la photogrammétrie. Ce savoir-faire a permis le développement des systèmes de télédétection et ď orthophotographie numérique et a, de plus, facilité la mise à disposition sur le marché de systèmes de photogrammétrie numérique qui sont maintenant pleinement opérationnels. On présente dans cet article ľ expérience acquise dans le domaine du balayage, de la production des orthophotographies et de la stéréocompilation, on en tire quelques conclusions et ľ on termine par un aperçu sur ľ‘avenir de la photogrammétrie numérique. 〈section xml:id="abs1-3"〉〈title type="main"〉Zusammenfassung Seit seiner Gründung im Jahre 1982 hat sich das Institut Cartogràfic de Catalunya mit digitalen Bildern beschäftigt. Obwohl digitate Bilder und Verfahren ursprünglich auf die Fernerkundung begrenzt waren, wurden sie schrittweise auf die Photogrammetrie übertragen bzw. mit ihr kombiniert. Zusätzlich zur Entwicklung von Fernerkundung und digitaler Orthophoto-Systeme hat diese Erfahrung die Einführung kommerziell verfügbarer digitaler photogrammetrischer Systeme erleichtert, die jetzt überall in der Praxis angewendet werden. Der Beitrag beschreibt die Erfahrung bei der Abtastung, der Herstellung von Orthophotos und bei der Stereoauswertung und gibt einige Schluβfolgerungen und Aussichten in bezug auf die Digitalphotogrammetrie wieder.

Notes: The paper entitled The Photogrammetric Society Analogue Instrument Project was published in The Photogrammetric Record, 14(82): 565–582 (October 1993) and contained, as Appendix A, an extract from the Archive concerned with the history of the Zeiss Stereoplanigraph in Great Britain. In The Photogrammetric Record, 14(83): 769–782 (April 1994), three further Appendices were published which dealt with the Williamson Multiplex equipment and other instruments. In this third extract, the history of five other instruments of the Multiplex type are described. This brings to a close that section of the Archive dealing with instruments using direct optical projection.〈section xml:id="abs1-2"〉〈title type="main"〉Résumé On a publicé dans le numéro 14(82) (pp 565–582) ďoctobre 1993 du“Photogrammetric Record”ľarticle intitulé“le projet de la Société (britannique) de Photogrammétrie sur les instruments analogiques” et inséré dans ľannexe A un extrait des Archives relatif a ľhistorie du Stereoplan-igraphe Zeiss en Grande-Bretagne. On a publicé dans le numéro 14 (83) ďavril 1994 du“Photogrammetric Record”(pp 769–782) trois annexes supplémentaires qui traitaient de ľappareil Multiplex de Williamson et ďautres instruments. Dans ce troisième extrait, on présente ľhistorie de cinq autres appareils du type Multiplex. Cette partie des Archives qui traitait des appareils basés sur ľemploi ďuneprojection optique directe, se trouve ainsi achevee.〈section xml:id="abs1-3"〉〈title type="main"〉Zusammenfassung Der Beitrag mit dem Titel“The Photogrammetric Society Analogue Instrument Project”wurde im Photogrammetric Record, 14(82): 565–582 (Oktober 1993) veröffentlicht und enthielt als Anlage A einen Extrakt vom Archiv, das mit der Geschichte des Zeiss Steroplanigraph in Groβbritannien verbunden war. Im Photogrammetric Record, 14(83); 769–782 (April 1984) wurden 3 weitere Anlagen veröffentlicht, die vom Williamson Multiplex und anderen Geräten handelten. In diesem 3. Auszug wird die Geschichte von 5 anderen Geräten des Multiplex-Typs beschrieben. Das führt zum Abschluβ desjenigen Teils des Archivs, das sich mit Geräten mit direkter optischer Projektion befaβt.

Notes: An analysis is given of the modelling of XY stage errors in an analytical plotter by polynomial functions. This is discussed as an alternative to demanding strict mechanical accuracy in the stage construction. In an era of plentiful computational resources, it is concluded that apportioning more of the responsibility to the mathematical co-ordinate refinement is an effective strategy. A second degree polynomial is used for each of the two stages in an analytical plotter, modelling the systematic stage errors to within the variability of a single observation. A requirement for instrument stability is emphasized. Such techniques have application for other instruments such as image scanners. 〈section xml:id="abs1-2"〉〈title type="main"〉Résumé On étudie la modélisation des erreurs des chariots XY ďun restituteur analytique en utilisant des fonctions polynomials. II s‘agit en fait de trouver une alternative à des exigences de prêcision mêcanique très strictes dans la réalisation de ces chariots. A une époque oùľon dispose ďune abondance de ressources en moyens de calcul, il apparait en effet relever ďune stratégie efficace de s'appuyer davantage sur les traitements mathématiques pour améliorer les valeurs des coordonnées. On utilise, pour chacun des deux chariots, un polynome du second degrè pour modéliser les erreurs systématiques des chariots, et les rendre inférieures aux erreurs propres à une observation unique. On insiste sur la nécessitéďune grande stabilityé instrumental. Ces processus peuvent également s'appliquer àďautres instruments comme les scanneurs ďimages. 〈section xml:id="abs1-3"〉〈title type="main"〉Zusammenfassung Es wird die Modellierung von Fehlern des xy-Wagens bei analytischen Auswertegeräten mit Hilfe von Polynomen analysiert. Des wird als Alternative zur Forderung nach einer strengen mechanischen Genauigkeit bei der Bildwagenkonstruktion diskutiert. Im Zeitalter hervorragender Computer-Ressourcen, so wird geschluβfolgert, ist es eine effektive Strategie, sich mehr der mathematischen Koordinatenverbesserung zuzuwenden. Ein Polynom 2. Grades wird für jeden der 2 Bildträger eines analytischen Auswertegerätes genutzt, um die systematischen Fehler im Bereich der Variation von einzelnen Beobachtungen zu modellieren. Bezüglich der Stabilität des Instruments wird eine Anforderung formuliert. Solche Verfahren kännen auch bei anderen Geräten, wie zum Beispiel Bildscanner angewendet werden.

Notes: Photogrammetry is used extensively in British Coal Opencast to provide surface profiles of both new sites and existing sites. This paper will discuss the development of GPS within Opencast and its deployment as a tool to provide the ground control required for mapping. Comparisons with conventional equipment will also be made and relative cost effectiveness assessed. 〈section xml:id="abs1-2"〉〈title type="main"〉Résumé On utilise largement la photogrammétrie dans British Coal Opencast, pour déterminer des profiles de surface, à la fois dans les sites nouveaux et dans les sites en cours. On examine dans cet article ľ emploi du GPS dans ces mines et son développement comme outil permettant ď obtenir les points ď appui sur le terrain nécessaires à la cartographie. On se propose defaire les comparaisons avec les équipements classiques et ďévaluer les avantages relatifs coût/efficacité. 〈section xml:id="abs1-3"〉〈title type="main"〉Zusammenfassung Die Photogrammetrie wird bei den Britischen Kohlen-Tagebauen extensiv zur Beschaffung von Oberflächenprofilen sowohl bei Neuaufschlüssen als auch bei bestehenden Gruben eingesetzt. Im Artikel wird die Entwicklung von GPS und seine Entwicklung als Hilfsmittel zur Beschaffung der für die Kartenherstellung benötigten Paβpunkte diskutiert. Es erfolgen auch Vergleiche mit der konventionellen Ausrüstung und in bezug auf die relative Kosteneffektivität.

Notes: An investigation has been made into the feasibility of using a low cost GPS receiver fitted to a light fixed wing aircraft as an aid to flying parallel flight lines for block aerial vertical photography. Two cases were examined: (I) the pilot controlled the heading of the aircraft according to the indications on the course deviation indicator of the GPS receiver; (2) the autopilot, being connected to the GPS receiver, controlled the heading of the aircraft. The results obtained for both sidelap and endlap were equal to or superior to those obtained with a CCTV system. 〈section xml:id="abs1-2"〉〈title type="main"〉RésuméOn a mené une recherche sur la faisabilité du GPS comme aide au maintien du parallélisme des axes de prises de vues d'un bloc de photographies aériennes verticales, en utilisant un récepteur GPS bon marché fié a un avion léger. On a examiné deux cas: (i) celui ou le pilote a maintenu le cap de l'avion en suivant les indications de l'index de changement de route du récepteur GPS; (ii) celui ou c'est le pilote automatique qui, par une liaison au récepteur GPS, a maintenu le cap de l'uvion. Les résultats obtenus pour le recouvrement latéral et l'extrgmité des bandes se sont révélés égaux ou supérieurs ii ceux obtenus avec un systPme CCTV.〈section xml:id="abs1-3"〉〈title type="main"〉ZusammenfassungEs wurde eine Untersuchung über die mögliche Nutzung billiger GPS-Empfanger, die in ein Kleinjlugzeug als Hilfsmittel zum Flug paralleler Bildstreifen für die blockweise Luftbildaufnahme eingebaut wurden, durchgefuhrt. Dabei wurden 2 Falle geprüft: (1) Der Pilot steuerte das Flugzeug entsprechend der Anzeige auf dem Kursindikator des GPS-Empfangers. (2) Der mit dem GPS-Empfänger verbundene Autopilot steuerte das Flugzeug. Die Ergebnisse sowohl für Querals auch Längsüberdeckung waren gleich oder besser als die mit einem CCTV-System erhaltenen.

Notes: The authors provide an account of current Directorate of Military Survey approaches to the provision of photocontrol by use of GPS and they pay special attention to the requirements of height control. 〈section xml:id="abs1-2"〉〈title type="main"〉SummaryGPS now provides us with a rapid method for determining plan and height for photogrammetric control directly over the points required by the photogrammetrist. By utilizing the slope of the geoid model, orthometric heights can be obtained to an accuracy of 0.15 m to 0.2 m. Surveys can be carried out quickly in all weather conditions and with none of the traditional terrestrial survey skills. Time, cost and manpower are greatly reduced. The plan accuracies are well within any requirements of the photogrammetrist and heights can be achieved to within 0.15 m to 0.2 m on test areas of UK. As photogrammetric height accuracy requires at best 1 m for plotting a 10 metre contour interval, heighting by GPS and the use of a geoid model solves the heighting problem.〈section xml:id="abs1-3"〉〈title type="main"〉Résumé Les auteurs fournissent un compte-rendu de la façon dont le Directorate of Military Survey aborde actuellement le problème de la fourniture de points ďappui photos avec ľemploi du GPS et prêterit une attention particulière à celui des points ďappui altimétriques.〈section xml:id="abs1-4"〉〈title type="main"〉Zusammenfassung Die Autoren geben einen Bericht über die gegenwärtigen Versuche beim Directorate of Military Survey zur Paβpunktbestimmung mit GPS, wobei sie den Anforderungen an die Höhenbestimmung besondere Aufmerksamkeit zollen.

Notes: The operational situation of precise airborne GPS positioning of camera air stations and its use in combined block adjustment for aerial triangulation is reviewed. The acceptance of systematic GPS errors, which occur especially with biased phase ambiguity solutions after loss of lock of signals in-flight, has motivated the method of additional linear GPS drift parameters which are to be solved in the combined block adjustment. It also implies that no absolute GPS co-ordinates are required and that the datum transformation is provided via a few ground control points. A great number of practical examples demonstrates that the method has been successfully applied within a wide range of photoscales and of block sizes. It has proved to be fully operational under various practical conditions. The high accuracy performance of GPS blocks and the economic benefit have been confirmed. It is particularly noticeable that the stationary receiver can be at a great distance, up to 500 km or more from the mission area. The conclusion is drawn that GPS supported aerial triangulation is ready to become standard. The potential improvement from dual frequency receivers and fast ambiguity solutions only enhances that conclusion. 〈section xml:id="abs1-2"〉〈title type="main"〉Résumé On examine, dans cet article, le degréďopérationnalité atteint par le GPS aéroporté dans le positionnement précis des sommets de prises de vues des caméras aériennes, et son insertion dans la compensation par blocs en aérotriangulation. La possibilityéďerreurs systématiques dans le GPS, en particulier en cas de perte des signaux en vol, qui se manifeste par des ambiguïtés de phase et des biais dans les résultats, à conduit a introduire dans la compensation en bloc des inconnues supplémental s, traduisant une dérive linéaire du GPS. II en résulte qu'il n'est pas nécessaire de disposer de coordonnées GPS absolues et que quelques points ďappui sur le terrain suffisent à assurer les changements de coordonnées. On a utilisé avec succès cette méthode dans de nombreux essaispratiques comportant un large eventail ďéchelles de prises de vues et de tallies des blocs. On a pu ainsi mettre en évidence ľentière opérationnalité de cette méthode, dans des conditions réelles différentes. On a également confirmé la haute précision des résultats des blocs GPS et les avantages économiques de ce système. En particulier, il est tout à fait remarquable que le récepteur fixe puisse se trouver à une grande distance de la zone du lasé, 500 km ou plus. On peut en conclure que ľaérotriangulation appuyée sur le GPS est en train de devenir une méthode standard. Les améliorations possibles que peuvent apporter les récepteurs à deux fréquences et la résolution rapide des ambiguïtés, ne font que renforcer cette conclusion. 〈section xml:id="abs1-3"〉〈title type="main"〉Zusammenfassung Die hochgenaue Kamera Positionierung durch GPS und ihre Anwendung für die Aerotriangulation wird zusammengefaβt dargestellt. Die Existenz systematischer GPS Fehler, wie sie inbesondere durch nur genäherte Phasen-Mehrdeutigkeitslösungen nach Signalabrissen im Flug verursacht werden, haben zu der speziellen Methode zusätzlicher linearer GPS Drift Parameter geführt, die in der kombinierten Blockausgleichung bestimmt werden. Ferner wird dabei die Datumtransformation mit Hilfe einiger weniger Paβpunkte bestimmt, d.h. es werden keine absoluten GPS Koordinaten vorausgesetzt. Mit einer groβen Zahl praktischer Beispiele wird demonstriert, daβ die Methode in einem groβen Bereich von Bildmaβstäben und Blockgröβen bereits erfolgreich angewendet wird. Sie hat sich unter den verschiedensten praktischen Bedingungen als voll operationell erwiesen. Dabei wurde die theoretisch erwartete hohe Genauigkeit der GPS Blöcke und ihre Wirtschaftlichkeit bestätigt. Besonders bedeutsam ist der empirische Nachweis, daβ der stationäre GPS Empfänger sehr weit, bis 500 km oder mehr, von dem Fluggebiet entfernt sein kann. Als Ergebnis wird empfohlen, die GPS gestützte Aerotriangulation als verfügbares photogrammetrisches Standardverfahren allgemein zu verwenden. Diese Schluβfolgerung wird durch die neuesten Entwicklungen von Zwei-Frequenz Empfängern und schnellen Mehrdeutigkeitslösungen, in die hohe Erwartungen gesetzt werden, nur noch bekräftigt.

Notes: A description is given of a heighting accuracy test carried out at Cartographical Services Limited on 1:2000 scale aerial photography. Premarked points, with ground surveyed heights, were established on a dual carriageway road near Worcester and photography was flown using a Leica RC20 aerial camera with EMC. Photogrammetric height measurement was carried out with both a Zeiss P3 analytical plotter and a Cartographic Engineering Limited/Carto Instruments AP190 plotter; respective r.m.s.e. accuracies of ± 25 mm and ± 30 mm were obtained. The new Cartographic Engineering Limited CP2 analytical plotter has subsequently been used for height measurement on similar photography and comparable accuracy has been achieved. 〈section xml:id="abs1-2"〉〈title type="main"〉Résumé On présente un essai de précision altimétrique, effectué par la Société“Cartographical Services” de Southampton, sur des photographies aérienne àľéchelledu 1:2000. On a utilisé des points pré-balisés, repartis sur une route à double voie près de Worcester, et dont ľaltitude a été déterminé sur le terrain. La prise de vue a été fait avec une caméra Leica RC20 munie ďun système de compensation du filé longitudinal. On a effectué les déterminations photogrammétriques des altitudes en utilisant à la fois un restituteur analytique Zeiss P3 et une restituteur AP190 de la Société“Cartographic Engineering/Carto Instruments.” On a obtenu comme erreurs moyennes quadratique les valeurs de ± 25 mm et ± 30 mm respectivement. On a enfin utilisé le noveau restituteur analytique CP2 de la Société“Cartographic Engineering” pour ces déterminations altimétriques et ľon a obtenu une précision comparable. 〈section xml:id="abs1-3"〉〈title type="main"〉Zusammenfassung Es wird ein Versuch zur Bestimmung der Höhengenauigkeit, der beim Cartographical Services Ltd. in Southampton für den Bildmaβstab 1:2000 durchgeführt wurde beschrieben. Signalisierte Punkte, deren Höhen im Gelände bestimmt wurden, wurden aufeiner Doppelfahrbahn in der Nähe von Worcester angeordnet undmit einerLeica RC20 mit EMC aufgenommen. Die photogrammetrische Höhenbestimmung erfolgte mit einem analytischen Auswertegerät P3 von Zeiss und einem AP 190 Plotter von Cartographic Engineering Ltd./Carto Instruments. Dabei wurden mittlere Fehler von ± 25 mm bzw. ± 30 mm erhalten. Das neue analytische Auswertegerät CP 2 von Cartographic Engineering Ltd. wurde danach für Höhenmessungen bei ähnlichen Luftbildern genutzt, wobei vergleichbare Genauigkeiten erreicht wurden.

Notes: This paper describes a method for modelling the SPOT satellite imaging process using a single Ground Control Point (GCP) and for extracting ground planimetric information. Satellite position is derived using the collinearity condition equations. Initial orbit is obtained from the given ephemeris data and refinement is carried out using an iterative least squares solution. Attitude variations are taken into account by fitting a third order polynomial in time to the attitudes derived from the ephemeris by integration. After refining the satellite position, planimetric co-ordinates can be derived using the collinearity equations with an accuracy of up to 38 m in latitude and 52 m in longitude (average of the r.m.s. errors from two tests) when the GCP used for modelling is obtained from the 1:50 000 scale map. These figures include the measurement errors as well as the modelling error. It has been found that the method is not sensitive to the location of the GCP used for modelling. Résumé On présente dans cet article une méthode de modélisation du traitement des images du satellite SPOT ne faisant appel qu'à un seul point ďappui au sol et permettant ďextraire des informations planimétriques. On obtient la position du satellite à partir des équations traduisant la condition de colinéarité. On part ďune orbite initiale issue des données fournies par les éphémerides, et on ľameliore par une résolution itèrative, basée sur les moindres carrés. On tient compte des variations ďattitude par ľintermédiaire ďun polynome du troisième ordre fonction du temps et correspondant aux attitudes dérivées des éphémérides par intégration. Une fois obtenue la position définitive du satellite, on peut tirer les coordonnees planimétriques des équations de colinéarité avec une précision qui atteint 38 m en latitude et 52 m en longitude (valeurs moyennes des erreurs moyennes quadratiques rèsultant de deux essais), lorsque le point ďappui au sol utilisé dans la modélisation provident de la carte au 1:50 000. Ces chiffres incluent les erreurs de mesure ainsi que ľerreur de modélisation. On a trouvé que la méthode nétait pas du tout sensible à la position qu'occupait le point ďappui au sol utilisé dans le modélisation. 〈section xml:id="abs1-2"〉〈title type="main"〉Zusammenfassung Im Artikel wird ein Verfahren zur Geländemodellierung des SPOT-Abbildungsprozesses unter Verwendung eines einzelnen Geländepaβpunktes (GCP) und zur Ableitung planimetrischer Informational beschrieben. Die Satellitenposition wird mit Hilfe der Kollinearitätsgleichungen beschrieben. Die Näherungswerte für die Bahnkurve werden aus gegebenen Ephemeriden bestimmt, die durch einen iterativen Prozeβ nach der Methode der kleinsten Quadrate verbessert werden. Veränderungen der Bahn werden rechtzeitig durch Anpassung mit einem Polynom 3. Grades an die aus den Ephemeriden abgeleitete Bahn durch Integration angepaβt. Nach Verbesserung der Satelliten-position können die Lagekoordinaten mit Hilfe der Kollinearitätsgleichungen mit einer Genauigkeit bis zu 38 m in der Breite und 52 m in der Länge (Mittelwert der mittleren Fehler aus 2 Versuchen) bestimmt werden, wenn der Paβpunkt (GCP) aus einer Karte 1:50 000 entnommen wurde. Dabei ist der Meβfehler ebenso wie der Modellierungsfehler enthalten. Es zeigte sich, daβ das Verfahren nicht empfindlich ist in bezug aufdie Lage des zur Modellierung benutzten Paβpunktes.

Notes: A method of delineating the extent of floods has been devised involving the establishment of flood surfaces and the creation of photogrammetric digital terrain models. These two data sources are combined in a computer aided map production system and comparisons are drawn between the efficacy of different forms of terrain modelling in a floodplain environment.〈section xml:id="abs1-2"〉〈title type="main"〉RésuméOn a mis au point, en vue de déterminer l'étendue d'innondations, une méthode numérique combinant les notions de surface innondable et de modèle numérique de terrain (MNT); on se pose le problème du choix du MNT dans un environnement de plaine innondable.〈section xml:id="abs1-3"〉〈title type="main"〉ZusammenfassungAngabe eines Verfahrens zur Ableitung der Ausdehnung von Überschwemmungen, wobei die Flut-Oberfläche bestimmt und photogrammetrisch digitale Geländemodelle erzeugt werden. Diese beiden Datenquellen werden in einem rechnergestützten Kartiersystem kombiniert, und es werden Vergleiche bezüglich der Wirksamkeit verschiedener Verfahren der Geländemodellierung in Flussniederungen gezogen.

Notes: The role of the cilia in the locomotion (“gliding”) of Tetrahymena thermophila in a semi-solid medium has been studied when cells were migrating in gradients of attractant. Video recordings and computer-aided motion analysis of migrating cells and their ciliary activity show that Tetrahymena thermophila migrate by swimming forward in semi-solid methyl cellulose, using their cilia. Ciliary reversals occur at certain intervals and cause a termination (“stop”) of cellular migration. Cells with reversed cilia resume forward migration when normal ciliary beating resumes. In gradients of attractants, cells migrating towards the attractant suppress ciliary reversals, which leads to longer runs between stops than in control cells. Cells migrating away from the attractant have a higher frequency of ciliary reversals than the control cells resulting in shorter runs. Stimulated cells adapt to a particular ambient concentration of attractant several times during migration in the gradient. Adaptation is followed by de-adaptation, which occurs during the “stop”. In the presence of cycloheximide, a strong inhibitor of chemoattraction, the attractant-induced suppression of ciliary reversal is abolished (cells become desensitized to the attractant). It is concluded that Tetrahymena has a short-term memory during adaptation. This is important for the efficiency of migration towards an attractant.

Notes: Descriptive information is provided for five ciliates collected from marine coastal British Columbian surface waters. In this paper we identify three new species, Strobilidium neptuni n. sp., Strobilidium veniliae n. sp., and Strombidinopsis multiauris n. sp.; provide a new name and description for one previously described species, Strombidium siculum nom. nov. and provide more taxonomic data for Strombidinopsis cheshiri Snyder and Ohman, 1991.

Notes: Giardia lamblia trophozoites contain acetylated α-tubulin but lack detectable levels of tyrosinolated α-tubulin, as demonstrated in immunoblots with monoclonal antibodies specific for these tubulin forms. By immunofluorescence microscopy, acetylated α-tubulin is localized in axonemes, median bodies and in the adhesive disk. Post-embeddment immunogold labeling of thin sections of cells was used to evaluate acetylation at the level of individual microtubules by electron microscopy. Cells were fixed with glutaraldehyde and embedded in the acrylic resin LR Gold. Results indicate all microtubules in adhesive disk, axonemes, basal bodies, funis and the median bodies contain acetylated α-tubulin. Unlike immunofluorescence labeling, all microtubules of the adhesive disk and the funis could be gold labeled. No nonspecific labeling of the cytoplasm or of structures other than microtubules was observed. Acetylated microtubules in G. lamblia do not appear to be a subset of microtubules and acetylation appears uniform along the entire length of individual microtubules. Acetylation and the tyrosinolation state of microtubules in Giardia are discussed in the context of microtubule stability and crosslinked features of the cytoskeleton.

Notes: A model for the in vivo evaluation of antipneumocystis drugs has been developed in SCID mice infected intratracheally with cryopreserved mouse-derived Pneumocystis carinii. The development of a highly reproducible fatal P. carinii pneumonia occured within 10 weeks (mean survival time ± SEM = 72.2 ± 1.2 days). Continuous administration of dexamethasone (2 mg/liter in the drinking water) exacerbated the rate of onset of severe P. carinii pneumonia (mean survival time ± SEM = 63 ± 1.3 days) in SCID mice. The number of cysts per g of lung homogenate (homogenate counts) were maximal with an inoculum of 20,000 cysts at 6 weeks post infection. Homogenate counts correlated with infection scores (graded assessments of immunofluorescent cysts on lung impression smears) suggesting that infection scoring accurately and rapidly reflects the severity of P. carinii pneumonia in SCID mice. These studies led to the development of a drug screening protocol in which Pneumocystis-free female SCID mice (20–25 g) were started on dexamethasone 7 days prior to IT inoculation with a single dose of 20,000 cysts. Drugs were evaluated either for: a) prophylaxis (continuously from day 1 post infection) or b) treatment (from day 21 post infection) until day 42 post infection, when all mice were killed and infection scores determined. Co-trimoxazole (at 250 mg sulfamethoxazole + 50 mg trimethoprim/kg/day) given in the drinking water was found to be highly effective in both the prophylaxis and treatment of mouse P. carinii pneumonia. Co-trimoxazole remained very effective in the prophylaxis P. carinii pneumonia in the SCID mouse at 125 mg sulfamethoxazole + 25 mg trimethoprim/kg/day p.o. and showed some enhancement of efficacy over sulfamethoxazole alone at 125 mg/kg/day p.o., suggesting limited synergy between sulfamethoxazole and trimethoprim. The results presented provide confirmation of the usefulness and predictability of the model.

Notes: Immunofluorescence was used to reveal the presence of a rhodopsin-like molecule on the plasma membrane of the photoresponsive ciliate Fabrea salina. The ciliate, fixed on a glass support, showed a spontaneous red-orange fluorescence. Cells incubated with a fluoresceinated, anti-bovine opsin, sheep polyclonal antiserum showed instead a green fluorescence typical of fluorochrome excited at 436 nm, whereas cells incubated with a fluoresceinated, nonimmune sheep serum kept their original red fluorescence. Excess bovine opsin did not inhibit the antibody reaction, but detergent-coated purified bovine opsin was proven able to bind to the cell membrane. Since rhodopsin belongs to a gene-related protein family, which includes adrenergic or cholinergic receptors, the cell response to acute exposure to muscarinic and adrenergic agonist and antagonist drugs was investigated. Acetylcholine, atropine, hexamethonium, noradrenaline, or phenoxybenzamine in the culture medium (10-4 M) did not influence motion behaviour nor phototaxis of Fabrea salina. These findings raise the possibility that a rhodopsin-like molecule is present on the surface of Fabrea salina. Nevertheless, they do not prove that this surface antigen is a rhodopsin, or that this molecule is responsible for photoresponsiveness in this organism.

Notes: The life cycle of malaria parasites in the mosquito vector is completed when the sporozoites infect the salivary gland and are ready to be injected into the vertebrate host. This paper describes the fine structure of the invasive process of mosquito salivary glands by malaria parasites. Plasmodium gallinaceum sporozoites start the invasion process by attaching to and crossing the basal lamina and then penetrating the host plasma membrane of the salivary cells. The penetration process appears to involve the formation of membrane junctions. Once inside the host cells, the sporozoites are seen within vacuoles attached by their anterior end to the vacuolar membrane. Mitochondria surround, and are closely associated with, the invading sporozoites. After the disruption of the membrane vacuole, the parasites traverse the cytoplasm, attach to, and invade the secretory cavity through the apical plasma membrane of the cells. Inside the secretory cavity, sporozoites are seen again inside vacuoles. Upon escaping from these vacuoles, sporozoites are positioned in parallel arrays forming large bundles attached by multilammelar membrane junctions. Several sporozoites are seen around and inside the secretory duct. Except for the penetration of the chitinous salivary duct, our observations have morphologically characterized the entire process of sporozoite passage through the salivary gland.

Notes: CLARKE BRADBURY, Department of Zoology, North Carolina State University, Raleigh, North Carolina 27695-7617.

Notes: Hybridization using kDNA and rDNA sequences as probes was performed to study phylogenetic relatedness of different species of trypanosomatids. Using this approach, we identified five organisms which had been classified as Phytomonas and Herpetomonas that were more closely correlated to each other phylogenetically than to any other species or isolates from either genera. These findings raise doubts about the validity of the current classification of Trypanosomatidae. Finally, we demonstrated the usefulness of kDNA sequences as an alternative to genomic sequences in obtaining phylogenetic information on trypanosomatids.

Notes: The relative capacity of Na+, K+ and Cl- to stimulate germination of spores of the microsporidian Nosema algerae, a pathogen of mosquitoes, was examined by ion substitution experiments. Sodium at 0.1 M was ineffective to produce the high percentage of germination that typically occurs with 0.1 M NaCl (the normal stimulation solution) if Cl- was substituted with the usually impermeant anions SO42-, HPO42-, or the organic acids oxalate, cacodylate, EGTA, MES and HEPES. However, substantial concentration- and pH-dependent germination was seen with Na2SO4 in the 0.2-0.8 M Na+ range. Similar results were obtained with solutions of K+ accompanied by impermeant anions. In contrast, the chloride salts of usually impermeant cations, like choline and triethanolamine, failed to germinate spores even at 0.8 M unless Na+ or K+ was independently added. The presence of 0.5 M choline chloride in the medium reduced the levels of Na2SO4 required to produce germination down to equivalence with those of Na+ in the normal stimulation solution. Monensin, a Na+ ionophore, facilitated the germination induced by a medium-level stimulus (0.04 M NaCl) in sonicated samples. These findings indicate that N. algerae spores germinate in response to the alkali metal cations, while CI- plays a passive role by diffusing to maintain internal electroneutrality during cation influx. A possible mechanism of cation action in spore germination is suggested on the basis of these results and observations on other systems of intracellular motility.

Notes: Certain monoclonal antibodies interact with proteins of Tetrahymena thermophila found in the conjugation junction as well as around the gametic nuclei (pronuclei) of conjugating cells; they also react with the oral primordium and fission zone of vegetative cells and with the cytoproct and contractile vacuole pores of all cells. One of these (FXIX-3A7) was investigated in detail. Immunogold labelling suggests that the material labelled by the 3A7 monoclonal antibody, which we call “fenestrin,” is located beneath the epiplasm (membrane skeleton). Immunoblots reveal that the major and perhaps sole antigen is a 64 kDa polypeptide, found in two isoelectric variants. Developmental studies implicate fenestrin in two processes involved in conjugation. The first is “tip transformation.” During preliminary starvation (“initiation”), labelling of fenestrin first appeared as a spot at the anterior end of starved mature cells, then after mixing of different mating types (“costimulation”) it extended posteriorly along the anterior suture. After pairing, this region spread to form a widened plate. The second process is pronuclear transfer. Fenestrations representing channels between the conjugating cells began to appear 0.5 to 1 h after the conjugants united, and eventually merged to form a small number of temporary large holes during exchange of the transfer pronuclei. A fenestrin envelope also enclosed both the transfer and resident pronuclei; a strand of fenestrin connected the two. Shortly after pronuclear transfer, both transfer and resident pronuclei were released from fenestrin caps and fused to produce a zygotic nucleus (synkaryon) not associated with fenestrin. Fenestrin thus appears to be intimately involved in the process of pronuclear exchange.

Notes: . The ultrastructure of meiospores of six different species of Amblyospora found infecting larval mosquitoes of Aedes abserratus, Aedes aurifer, Aedes cinereus, Aedes excruciates, Aedes sticticus, and Aedes stimulans are described. Meiospores of all species exhibited characteristics typical for the genus Amblyospora including: a single nucleus, a large posterior vacuole, a thick undulating exospore and thinner endospore, a bipartite and lamellate polaroplast with more tightly stacked membranes in the proximal region, and an anisofilar polar filament with distal coils reduced in thickness. Distinct differences were found in the arrangement and number/ ratio of coils formed by the broad basal and narrow distal portions of the polar filament. These differences, when quantified and averaged, were unique from all other mosquito-parasitic species that have been examined ultrastructurally. Information on parasite development, natural field prevalence and transmission to suspected intermediate copepod hosts is presented. The creation of six new species, Amblyospora abserrati, Amblyospora auriferi, Amblyospora cinerei, Amblyospora excrucii, Amblyospora stictici, and Amblyospora stimuli is proposed. Synonymies and complete host lists of all forms and species of Amblyospora described from mosquitoes are given.

Notes: . The α- and the β-tubulin genes of the hypotrichous ciliate Euplotes octocarinatus were isolated from a size-selected macronuclear DNA library. The α-tubulin gene is located on a 1,587 bp macronuclear DNA molecule and the β-tubulin gene on a 1,524 bp macronuclear DNA molecule. Sequencing revealed that all the cysteine residues of the two genes are encoded by the common cysteine codons UGU and UGC and none by an UGA codon. This is in contrast to the genes of E. octocarinatus sequenced so far, where some of the cysteines are encoded by the opal codon UGA. The tubulin genes end like other Euplotes genes with a TAA. They do not contain introns. The last codon for an amino acid in the α-tubulin gene is a GAA which codes for glutamic acid. This is in contrast to what has been reported for most α-tubulin genes, but it supports findings for other hypotrichous ciliates. No evidence for the existence of more than one type of α- and one type of β-tubulin genes could be obtained.

Notes: Intrachromosomal variant surface glycoprotein (VSG) genes in Trypanosoma brucei are expressed by a mechanism involving gene conversion. The 3’boundary of gene conversion is usually within the last 130 bp of the VSG gene, a region of partially conserved sequences. We report here the loss of the predominant telomeric A VSG gene in the cloned variant antigenic type (VAT) 5A3, leaving only an intrachromosomal A VSG gene (the A-B gene). The nucleotide sequence of the A-B VSG gene reveals that it lacks the normal VSG 3′ sequence. Surprisingly, we find cells expressing this A-B VSG gene in relapse populations arising from VAT 5A3. Since the A VSG mRNAs from these cells have a normal 3′ sequence, the incomplete A-B VSG gene must be expressed via a partial gene conversion that supplies the functional 3’end. Although the A-B VSG gene is no longer predominant like the telomeric A VSG gene, it is still expressed more frequently than other intrachromosomal VSG genes, suggesting that factors other than a telomeric location determine whether a VSG gene is expressed early in a serodeme.

Notes: Mixed life cycle stages of rat-derived Pneumocystis carinii were isolated from host lungs and their sterols were compared with those present in lungs from normal and immunosuppressed uninfected rats. Gas-liquid chromatography consistently detected, resolved, and quantified 9, 10, and 20 sterol components in the total nonsaponifiable neutral lipid fraction of lungs from normal rats, lungs from immunosuppressed uninfected rats, and P. carinii preparations, respectively. In all samples, cholesterol was the most abundant sterol present, comprising 97%, 93%, and 78% of total sterols in lungs from normal rats, lungs from immunosuppressed uninfected rats, and P. carinii, respectively. Tentative identifications of several rat lung and P. carinii minor sterols were made based on gas-liquid chromatogram retention times and fragmentation patterns from mass spectral analyses. Campesterol (ergost-5-en-3-ol), cholest-5-en-3-one, and β-sitosterol (stigmast-5-en-3-ol) were among the minor components present in both types of lung controls, and were also components of P. carinii sterols. In contrast to lung controls, the sterols of P. carinii were enriched in C28 and C29 sterols with one or two double bonds, and a hydroxyl group at C-3 (ergost-5-en-3-ol, ergost-7-en-3-ol, ergosta-dien-3-ol, stigmast-5-en-3-ol, stigmast-7-en-3-ol and stigmasta-dien-3-ol). Steryl esters of P. carinii, probably stored in cytoplasmic lipid droplets, were dominated by those present in the host lung. In separate studies. 3-hydroxy-3-methylglutaryl coenzyme A activity, a key enzyme in the regulation of sterol biosynthesis, was detected in purified P. carinii preparations and incorporation of radiolabeled squalene and mevalonate was observed. Together, these results suggest that the parasite readily takes up and incorporates host sterols, and that the organism synthesizes some of its own “metabolic sterols”

Notes: SYNOPSIS. Thymidylate synthetase (E.C.2.1.1.45) has been demonstrated in unsporulated oocysts of Eimeria tenella. The properties of this enzyme have also been investigated in Tetrahymena pyriformis, as a protozoan model, and 7-day-old chick embryo, as a host model. The enzymes from E. tenella and chick embryo were inhibited by all concentrations of MnCl2 and MgCl2 tested. Tetrahymena pyriformis thymidylate synthetase was stimulated by low concentrations of both these cations but was inhibited by high concentrations. Subsequent data refer to chick embryo, E. tenella and T. pyriformis respectively: the apparent Km was 5.89 μM, 5.94 μM, and 0.53 M for the substrate dUMP: and 5.13 μM, 1.10 μM and 4.65 μM, respectively for the cofactor N5N10-methylenetetrahydrofolate. The pH optimum for the enzyme from both chick embryo and T. pyriformis was 8.0, with Tris-HCl buffer; activity of E. tenella thymidylate synthetase was still increasing at pH 8.2. The E. tenella enzyme was found to have a molecular weight of 4.6–4.9 × 105 daltons. The effects of nucleotides, inhibitors, and the omission of assay components on each enzyme are presented. Thymidylate synthetase from E. tenella is not greatly different from that of chick embryo, but does not resemble the enzyme from T. pyriformis. A case for using thymidylate synthetase as a chemotherapeutic target in the treatment of Eimeria infections remains. Indeed Eimeria may be considered as a model for infections caused by other protozoan parasites, such as Toxoplasma and Plasmodium, provided that suitable inhibitors can be found that are not toxic to the host.

Notes: SYNOPSIS. A protocol based on density differences between starved and fed cells and employing density gradient centrifugation has been devised to facilitate the isolation of auxotrophic mutants of cell lines derived from Tetrahymena thermophila strain B1868. First, a mass phenotype screening procedure was established whereby true auxotrophic mutants and slow-growing wild-type cells such as strain C* could readily be distinguished. Second, simulation experiments were performed in which wild-type cells starved first in non-nutritive buffer, then suspended in a defined medium lacking a single essential amino acid became significantly denser than the same cells when starved, then suspended in a complete defined medium. Finally, using the same protocol, a reconstruction experiment was carried out which resulted in effective separation of wild-type cells from cells of a tyrosine auxotroph. The overall procedure resulted in a 9-fold increase in the relative frequency of auxotrophic cells, while the density gradient centrifugation alone provided a 400-fold enrichment.

Notes: SYNOPSIS. Infectivity of Plasmodium gallinaceum (Brumpt) sporozoites isolated from midguts and salivary glands of experimentally infected Aedes fluviatilis (Lutz) was studied. The 2 populations were compared at 7, 8, and 9 days postisolation from mosquitoes, which were maintained at 27 C ± 1C and ∼75% relative humidity. Infectivity of the parasites was evaluated by the length of the prepatent period of the infection in 2-week-old chicks inoculated intramuscularly. Infection was caused by 7-day-old sporozoites from salivary glands, but not from midguts. Older sporozoites induced infection in all the inoculated chicks. The results suggested a somewhat higher infectivity of the 8- and 9-day salivary-gland parasites than of the oocyst sporozoites. However, unlike sporozoites from mammalian malaria, oocyst sporozoites from avian malaria were highly infective at this age.

Notes: SYNOPSIS. Sorogena stoianovitchae Bradbury & Olive, an epiphytic ciliate found in various parts of the world, has a trophic stage that feeds on members of the ciliate genus Colpoda. When grown in the presence of the food ciliate, it multiplies rapidly. When the cells become abundant they aggregate at the water surface on inserted plant fragments or floating pollen grains, the sides of culture dishes, or on floating films such as those deposited by bacteria or pollen grains. an aggregate mounds up and becomes ensheathed above the water level, after which the mass of cells called a sorogen rises aerially at the apex of a stalk deposited at its base. the tapering, noncellular stalk consists of a conspicuously furrowed sheath that encloses a mucilaginous matrix. At completion of stalk development the cells of the sorogen become encysted. the sorocysts are commonly discharged by fracturing of the drying sorus. Alternating light and dark conditions are required for sorocarp development.

Notes: SYNOPSIS. the antigenic types in populations of metacyclic trypanosomes of Trypanosoma brucei isolated from Glossina morsitans head-salivary gland trypanosome cultures and bloodstream forms in the early parasitemias produced from whole culture supernatant fluids containing metacyclic forms, were analyzed by the indirect fluorescent antibody test using clone-specific antisera. Metacyclic trypanosomes in cultures initiated with cloned bloodstream forms were heterogeneous with respect to their variable antigenic type (VAT). Trypanosomes comprising early parasitemias in immunosuppressed mice infected with metacyclics produced in cultures also had a range of VATs. Three of the VATs detected in the early parasitemias in mice have also been identified by other investigators in tsetse fly-transmitted populations of the same stock.

Notes: SYNOPSIS. A simple method is described for plating and cloning ciliates and other protozoa, based on a principle differing from that traditionally used for plating and cloning bacteria and other microorganisms. This procedure, referred to as the silicone-oil-plating-procedure (SOPP), involves vortexing small volumes of culture medium containing protozoa with larger volumes of a non-toxic silicone oil and plating the resulting unstable emulsion in small plastic petri plates. Discrete microdroplets of culture medium form containing protozoa entrapped and immobilized between the hydrophobic surfaces of the plastic petri dish and the oil. Protozoa, isolated by this method grow, divide, and multiply to form clones. the procedure may be used for plating and cloning protozoa in bacterized and axenic culture. Variations of the basic method may be applied to isolating protozoa from the wild, washing protozoa to remove microorganisms, screening for potential mutants, and for replica plating.

Notes: SYNOPSIS. Cyclic nucleotide phosphodiesterase [EC 3.1.4.17] was examined in Tetrahymena pyriformis strain NT-1. Enzymic activity was associated with the soluble and the particulate fractions, whereas most of the cyclic GMP phosphodiesterase activity was localized in the soluble fraction: the activities were optimal at pH 8.0–9.0. Although very low activities were detected in the absence of divalent cations, they were significantly increased by the addition of either Mg2+ or Mn2-. A kinetic analysis of the properties of the enzymes yielded 2 apparent KIII values ranging in concentration from 0.5 to 50 μM and from 0.1 to 62 μ M for cyclic AMP and GMP. respectively. A Ca2+-dependent activating factor for cyclic nucleotide phosphodiesterase was extracted from Tetrahymena cells, but this factor did not stimulate guanylate cyclase [EC 4.6.1.2] activity in this organism. On the other hand, Tetrahymena also contained a protein activator which stimulated guanylate cyclase in the presence of Ca2+, although this activator did not stimulate the phosphodiesterase. the results suggested that Tetrahymena might contain 2 types of Ca2+-dependent activators, one specific for phosphodiesterase and the other for guanylate cyclase.

Notes: SYNOPSIS. the cell size of Didinium nasutum was found to be dependent on the size of the Paramecium species available as prey. Didinium feeding on P. tetraurelia averaged 5.6 × 105μm3. the cell volume of Didinium increased with increasing prey size for the 5 prey species tested, to 9.1 × 105μm3 for Didinium feeding on P. caudatum. Didinium nearing a cell division ranged in size from 8.6 × 105μm3 on P. tetraurelia to 12.9 × 105μm3 on P. caudatum. the range in cell volume is such that Didinium feeding on P. caudatum are larger than the size at which Didinium divide when feeding on P. tetraurelia. This morphologic plasticity in cell volume allows Didinium to exploit a wide size range of Paramecium species as prey. It is proposed that the size of a Didinium may have profound effects on its ability to encounter and capture prey of different sizes.

Notes: RESUME. Chacun des 45–80 organelles adoraux de Bursaria truncatella O. F. Müller est constitué de 3 rangées de cinétosomes et l'aire buccale droite est couverte de nombreuses doubles rangées de cinétosomes. La stomatogenèse débute par la désorganisation et la résorption des organelles buccaux postérieurs. Puis, il y a désorganisation des rangées parorales de cinétosomes et multiplication des cinétosomes sur l'aire orale droite, en měme temps que sont rompues, selon une ligne oblique, un certain nombre de cinéties somatiques. La prolifération des cinétosomes aux extrémités des cinéties. de part et d'autre de la ligne de rupture, aboutit, d'une part, à la formation d'un champ anarchique qui est le primordium oral droit de l'opisthe, d'autre part, à la formation de nombreux doublets qui constituent chacun le primordium de chaque organelle adoral. Après la séparation des tomites, les cinétosomes de l'aire droite s'ordonnent en doubles rangées et les organelles adoraux se complètent par addition d'une 3ème rangée de cinétosomes. Les cinétosomes somatiques sont jumelés, reliés par 2 desmoses. Les fibres transverses postérieures et les fibres postciliaires forment de longs rubans de microtubules dirigés vers l'arrière et juxtaposés dans les crětes intercinétiennes. Les doubles rangées droites de cinétosomes buccaux sont assimilables à des stichodyades. Les organelles des cinétosomes adoraux portent des rideaux de fibres postciliaires convergents ou divergents. La rangée postérieure de chaque organelle est non ciliée. Par son type de stomatogenèse, par sa structure corticale, par l'ultrastructure des organelles adoraux, Bursaria appartient aux Colpodidea, ce qui suggère des remarques de plusieurs types.SYNOPSIS. In Bursaria truncatella O. F. Müller, each of the 45–80 adoral organelles is composed of 3 rows of kinetosomes, and the right buccal area is covered by many double rows of kinetosomes. Stomatogenesis begins by disorganization and disappearance of the posterior buccal organelles. Next, there is disorganization of the paroral rows of kinetosomes and multiplication of kinetosomes in the right oral area; at the same time, some somatic kineties are disrupted along an oblique line. Multiplication of kinetosomes at the extremities of the kineties, on both sides of the disruption, leads to the formation of an anarchic field which is the right oral primordium of the opisthe and the formation of doublets each of which constitutes an adoral organelle. After the separation of the tomites. the kinetosomes in the right buccal area position themselves, and the adoral organelles are completed by the addition of a 3rd row of kinetosomes. Somatic kineties are formed by successive pairs of ciliated kinetosomes united by 2 desmoses. the long posterior transverse ribbons and the postciliary ribbons extend posteriad, overlapping in the pellicular ridges. Oral rows of kinetosomes on the right can be compared with stichodyads. the adoral kinetosomes have convergent or divergent postciliary ribbons. the posterior row of kinetosomes in each organelle is not ciliated. By the type of stomatogenesis, the cortical ultrastructure, the ultrastructure adoral of its organelles, Bursaria belongs to the Colpodidea.

Notes: SYNOPSIS Free-living marine ciliates occur in the interstitial spaces of a wide vareity of filamentous and particulate substrata, on the surfaces of planar substrata, and in the plankton. In addition, they are found in association with a wide variety of plant and animal hosts. In this paper I review the progress during the past decade in understanding the distribution of marine ciliates, with particular emphasis on the relationship between ciliate biogeography and the species problem. It is concluded that as a general rule among marine ciliates, genera and species complexes are cosmopolitan. Specific locales may support a confusing array of sibling species or subspecific morphologic variants. Because the distributional processes and breeding biology of marine ciliates are only beginning to be understood, conventional ideas that marine ciliate species are cosmopolitan may require modification.

Notes: SYNOPSIS The subkingdom Protozoa now includes over 65,000 named species, of which over half are fossil and ∼ 10,000 are parasitic. Among living species, this includes ∼ 250 parasitic and 11,300 free-living sarcodines (of which ∼ 4,600 are foraminiferids); 1.800 parasitic and 5,100 free-living flagellates: ∼ 5,600 parasitic “Sporozoa” (including Apicomplexa, Microspora, Myxospora, and Aseetospora); and ∼ 2,500 parasitic and 4,700 free-living ciliates. There are undoubtedly thousands more still unmamed. Seven phyla of PROTOZOA are accepted in this classification—SARCOMASTIGOPHORA. LABYRINTHOMORPHA, APICOMPLEXA, MICROSPORA, ASCETOSPORA, MYXOSPORA, and CILIOPHORA. Diagnoses are given for these and for all higher taxa through suborders, and representative genera of each are named. the present scheme is a considerable revision of the Society's 1964 classification, which was prepared at a time when perhaps 48,000 species had been named. It has been necessitated by the acquisition of a great deal of new taxonomic information, much of it through electron microscopy. It is hoped that the present classification incorporates most of the major changes that will be made for some time. and that it will be used for many years by both protozoologists and non-protozoologists.

Notes: SYNOPSIS Leishmania donovani amastigote-to-promastigote transformation is inhibited by homogenates of infected hamster liver and spleen. This inhibitory activity is localized in the 100,000 g pellet fraction. Tests with lysates of adherent (macrophyages) and nonadherent (lymphocytes) spleen cells indicated that the inhibitory activity resided in the lymphocytes, specifically in the 100,000 g pellet fraction.

Notes: SYNOPSIS Antibodies induced in rabbits against Paramecium multimicronucleatum syngen 2 prevent sexually reactive cells from clumping, pairing, and forming cytoplasmic fusions. A biologic assay for the detection of these antibodies (designated blocking antibodies) is described. the blocking antibodies, unlike the immobilization antibodies, are produced against breis of sexually reactive cells and nonreactive cells of 2 types, nonstarved and immature. Isolated cilia from reactive cells of either mating type are weak immunogens for blocking antibodies. No correlation between the mating type specificity (III or IV) and these antibodies has been detected. Blocking antibodies can be absorbed with living cells, of which sexually reactive ones are the most effective absorbers, while immature ones are the least effective.

Notes: SYNOPSIS A method is described for the axenic mass cultivation of Paramecium tetraurelia strains 51s and 299s. the ciliate is grown in an enriched axenic medium developed by Soldo, Godoy & van Wagtendonk. Under continuous shaking on a rotary shaker, cultures were grown in one-liter Erlenmeyer flasks with 330 ml medium yield cell densities of 32,000 cell/ml and 20,000 cells/ml for strains 299s and 51s respectively. Doubling time is considerably shorter under these conditions than in the conventional static cultures. A 20-liter airlift bioreactor is described in detail which can be used successfully to otain up to 100 g wet weight of Paramecium in a single run; in this reactor the cell density reaches 38,000 cells/ml for strain 299s. and 23,000 cells/ml for 51s. This technic should facilitate the study of minor protein components of the ciliate.

Notes: SYNOPSIS The cadmium ion (Cd2+) was accumulated by Amoeba proteus in all cellular fractions, the highest level being associated with the cytosol fraction. On gel separation of the cytosol fraction, Cd-binding protein appeared in 2 peaks: one 〉45,000 MW (peak I) and the other 12,000 MW (peak II). Added cysteine increased the total Cd2+ taken up by the cells and resulted in disproportionate increase of Cd incorporated into the Cd-binding protein of peak II. the Cd-binding protein of peak II is analogous to the low-MW, Cdbinding proteins in Anacystis nidulans, Mytilus edulis, and to the metalloprotein of some vertebrates.

Notes: Cultured P. carinii rapidly took up a variety of fatty acids. The relative rates of uptake for four fatty acids were 18:1 〉〉 16:0 ≅ 18:0 ≅ 18:2. Fatty acids were primarily incorporated into phospholipids and the uptake process was specifically inhibited by 2.2 and 22 μM primaquine, a concentration having no effect on host cells. Amino acids were also taken up by cultured P. carinii in a primaquine sensitive process. Radiolabeled leucine was incorporated into the major surface glycoprotein of P. carinii. The formation of radioactive P. carinii-specific proteins indicated that the cultured organisms carried out transcription and translation and that the incorporation of amino acids was dependent upon P. carinii rather than rare HEL human embryonic lung cells. The spinner flask culture system provides convenient access to P. carinii for metabolic studies in defined medium for a period of 5–14 days after inoculation.

Notes: The roles of the myosin I class of mechanoenzymes have been investigated by single and double gene knockout studies in the amoeba Dictyostelium discoideum. Cells lacking different myosin I pairs (myoA-/myoB-, myoB-/myoC-, and myoA-/myoC-) were examined with respect to their cytoskeletal organization. F-actin localization by rhodamine-phalloidin staining of cells indicates that the myoA-/myoB-, myoB-/myoC-, and myoA-/myoC- cells appear to redistribute their F-actin more slowly than wild type cells upon adhesion to a substrate. These studies suggest that Dictyostelium myoA, myoB, and myoC may have overlapping roles in maintaining the integrity or organization of the cortical membrane cytoskeleton.

Notes: The new microsporidium, Napamichum cellatum, a parasite of the adipose tissue of midge larva of the genus Endochironomus in Sweden, is described based on light microscopic and ultrastructural characteristics. Plurinucleate Plasmodia with nuclei arranged as diplokarya divide, probably by plasmotomy, producing a small number of diplokaryotic merozoites. The number of merogonial cycles is unknown. Each diplokaryotic sporont yields eight monokaryotic sporoblasts in a thin-walled, more or less fusiform sporophorous vesicle. A small number of multisporoblastic sporophorous vesicles were observed, in which a part of the sporoblasts were anomalous. The sporogony probably begins with a meiotic division. The mature spores are slightly pyriform. Fixed and stained spores measure 2.1-2.4 × 3.7-4.5 μm. The five-layered spore wall is of the Napamichum type. The polar filament is anisofilar with seven to eight coils (142-156 and 120 nm wide). The angle of tilt is 55-65°. The polaroplast has an anterior lamellar and a posterior tubular part. The granular, tubular and crystal-like inclusions of the episporontal space disappear more or less completely when the spores mature. The crystal-like inclusions are prominent in haematoxylin staining, but not visible with the Giemsa technique. The microsporidium is compared to other octosporoblastic microsporidia of midge larva and to the species of the genera Chapmanium and Napamichum.

Notes: CB1-glycoprotein is a component of flagellar pocket, endosome, and lysosome membranes of long, slender bloodstream forms of the Trypanosoma brucei subgroup of African trypanosomes. We have used immunoblotting, immunofluorescence, and cryoimmunoelectron microscopy to study CB1-glycoprotein expression as long, slender bloodstream forms of pleomorphic T. b. brucei and T. b. gambiense transform through intermediate stages into short, stumpy forms. Intermediate and stumpy forms express more CB1-glycoprotein than long, slender forms. These results, coupled with previous work showing that procyclic forms do not express CB1-glycoprotein, show that the expression of lysosomal membrane glycoproteins is regulated coordinately with other aspects of lysosome and endosome function as these trypanosomes go through their life cycle.

Notes: Trichocyst discharge is an effective defense of Paramecium against Dileptus margaritifer. The possible defensive function of backward swimming, which often follows trichocyst discharge upon Paramecium-Dileptus encounters was studied. Mutants incapable of backward swimming (pawnA in P. tetraurelia, cnrA in P. caudatum) escaped from dilepti nearly as frequently as wild-type cells. Double mutants (pawnA-nd7, cnrA-tnd2) were eaten nearly as frequently as mutants incapable of trichocyst discharge. Thus, in the defense of Paramecium against D. margaritifer, the role of backward swimming is minor, if any, compared to trichocyst discharge. Among escaped cells, about a half of wild-type and essentially none of pawnA (cnrA) cells showed backward swimming. Paramecium behavior during the encounter can be mimicked by the local, not global, application of lysozyme which is a strong secretagogue of trichocyst.

Notes: A total of 4.1% infestation with Chilodonella spp. was found among fish studied in 144 tanks in 1987–1989, representing 14.0% of the tanks in which fish are reared at four salmonid farms in northern Finland. Two species were found, C. hexasticha and C. piscicola, and both occurred on salmon (Salmo salar L.), sea trout [S. trutta m. trutta (L.)] and brown trout [S. t. m. lacustris (L.)]. Variability was observed in the length and width of the C. piscicola specimens and the number of ciliary rows or kineties. Large specimens which had more kinetics than average for C. piscicola were found mainly on the skin of salmon aged 1–2 years. The number of kinetics in the right ciliary band was found in stepwise logistic regression analysis to be of importance when typing C. piscicola specimens. Fingerlings were found to be more susceptible to Chilodonella infestation than older fish, and mortality varied in the range 2–10% in the course of the epizootics in the three fish species. Most mortality cases were caused by C. hexasticha, occurring mainly on the gills of the fish. Chilodonella piscicola was most often found in salmon and occurred at lower water temperatures than C. hexasticha (mean water temperature when found for the first time being 13° C and 16° C, respectively).

Notes: Chlamydomonas reinhardtii exhibits photophobic and positive and negative phototactic responses that can be defined for cell populations using computerized cell tracking and motion analysis. Mutants CC-2359 and FN68 are pigment deficient mutants that are blocked in carotenoid synthesis and lack these photo responses. In particular, neither mutant exhibits flash-induced photophobic responses to visible light stimuli to which wild-type gametic cells exhibit a strong response, with several behavioral stages. Upon addition of all-trans retinal to these mutants, the photophobic responses are restored with minor quantitative differences from wild-type populations. Using both light and electron microscopy, we have compared the ultrastructural characteristics of wild-type C. reinhardtii to those of both mutants. As previously described, wild-type cells contain an eyespot consisting of 2–4 layers of pigmented granules encased within thylakoid membranes, located between the distal extremities of the flagellar root. This structure is also visible as an orange-red spot in light microscopy. The photoreceptor is thought to be concentrated in the plasma membrane above the eyespot. The mutant, CC-2359, lacks this eyespot as seen by both light and electron microscopy, even when the photophobic response has been restored. FN68-like mutants studied earlier by Morel-Laurens and Feinlieb and others contain an eyespot which can be seen only by electron microscopy. In FN-68, the eyespot generally has the same dimensions as in wt cells, differing mainly in pigment granule appearance. Consistent with these findings, several laboratories have shown that the full range of phototactic responses can be reconstituted in FN68 and CC-2359, but that negative phototaxis requires a significantly stronger light stimulus in the latter strain. We confirm the suggestion that the eyespot is not necessary for the photophobic response, and is not critical for the appropriate assembly and function of the photophobic response receptor in the membrane. Furthermore, the locus of reconstitution of the functional receptor is not the eyespot. Because of the definitive demonstration of the absence of the eyespot in CC-2359, however, the eyespot may play a role in negative phototaxis.

Notes: The rDNA repeat unit from a new human Giardia duodenalis strain shows significant differences from the previously reported G. duodenalis rDNA repeat. Twelve base-pair changes occurred in 490 bp of the SSrRNA gene and new restriction enzyme sites occurred in the LSrRNA gene. The overall length of the rRNA genes is the same but the spacer is 76 bp longer than previously reported. A boundary within the spacers of the two different rDNA units divides a region of 50% homology near the LSrRNA gene from a region of 80% homology toward the SSrRNA gene. This boundary region includes two copies of a 78 bp repeat.

Notes: Twenty-one different strains of Euplotes of the cirrotype 10, double dargyrome morphotype were collected in marine and brackish habitats from widely varying geographic locations. The strains were cultured under similar conditions prior to fixation and staining. The morphological features considered included the cell lengths and widths, the number and position of all the ventral cirri, adoral membranelles and dorsal cilia, and the position of the peristomal shelf. Univariate and multivariate analyses revealed continua in these measurements among the strains, as well as significant correlations between cell length and the number of adoral membranelles, the number of dorsal cilia, and the length, width and area of the oral cavity. Based on data from this study and from previous studies, we conclude that the traditional criteria used for species descriptions within this morphotype are insufficient to provide robust distinctions. Therefore, we submerge all of the descriptions within this morphotype under the nomenclaturally superior Euplotes charon Müller, 1786.

Notes: The Hartmannella vermiformis small-subunit rRNA coding region was amplified, and the amplified DNA was cloned and sequenced. The coding region is 1,840 nucleotides long, and is typical of eukaryotic rRNA genes in both size and composition. Different clones contained different nucleotides at three positions.

Notes: The micronuclear version of the gene encoding β-telomere binding protein (β-TBP) in Oxytricha nova has been sequenced and compared to the macronuclear β-TBP gene, previously described. The micronuclear gene contains three AT-rich internal eliminated sequences (IES) of 37, 40, and 43 bp and four macronuclear destined sequences (MDS). The IES interrupt the gene once near the 5′ end of the coding region and twice in the 3′ trailer downstream from the TGA stop codon. The sequences of the micronuclear and macronuclear genes are colinear. Thus, the micronuclear β-TBP gene is not scrambled, which contrasts with the highly scrambled state among the 14 MDS in the micronuclear α;-TBP gene.

Notes: A new species of marine interstitial ciliate, Tracheloraphis primitarum sp. n., is described from intertidal sands (160-200-μm medium grain size) along the Northwest Atlantic coast. Living specimens are not transparent under incident light, 0.4-0.9 mm in length, and are characterized by an elongated body, bulbous anterior and rounded posterior regions. Cytostome is simple without a cleft on the lip. Protargol silver impregnation shows 18-29 longitudinal kineties. The glabrous zone is very narrow, 6-7 μm wide, and corresponds to the area occupied by one kinety and two interkinetal spaces. Ten to fifteen kineties terminate against the glabrous zone. Interkinetal extrusomes are present. The nuclear apparatus is a single centrally-placed nuclear group that consists of three large irregular macronuclei and 2-3 micronuclei; these are either clustered or encapsulated.

Notes: The most commonly encountered protostelids are nominal members of the genus Protostelium Olive & Stoianovitch. These are Protostelium mycophaga Olive & Stoianovitch and P. irregularis Olive & Stoianovitch. Both species share the common features of long-stalked fruiting bodies with single, uninucleate, deciduous spores and trophic states that consist solely of uninucleate amoebae, but they are quite different with respect to their detailed structure at both the light and electron microscopic levels. Based on this evidence, it seems unlikely that the species are congeneric, and it is proposed that P. irregularis be assigned to Soliformovum n. g. Examination of other species of Protostelium indicates that P. nocturnum Spiegel should be retained in the genus and that P. expulsum Olive & Stoianovitch should be reassigned to Soliformovum. The group most closely related to Protostelium, under this new concept, is the genus Planoprotostelium Olive & Stoianovitch, while Soliformovum is best treated as a genus of Eumycetozoa incertae sedis, though it does share some similarities with the group that includes the genera Ceratiomyxella Olive & Stoianovitch, Nematostelium Olive & Stoianovitch, and Schizoplasmodium Olive & Stoianovitch.

Notes: Dyer, B. D. & Obar, R. A. 1994. Tracing the History of Eukaryotic Cells. The Enigmatic Smile. Dyer, B. D. & Obar, R. A. 1994. Tracing the History of Eukaryotic Cells. The Enigmatic Smile. Plattner, H. 1993. Advances in Cell and Molecular Biology of Membranes.