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  • Articles  (150)
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  • 101
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    Cell & tissue research 208 (1980), S. 507-510 
    ISSN: 1432-0878
    Keywords: Pancreatic B-cell ; Tubuloampullar structures ; Rough endoplasmic reticulum ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Unusual aggregations of ampullar dilated tubules (tubuloampullar structures, TAS) were observed in pancreatic B-cells of rats and in one case of human malignant insulinoma. The TAS were invariably associated with dilated rough endoplasmic reticulum, but lacked ribosomes. They were frequently seen to be closely associated with the Golgi apparatus. Furthermore, TAS were located mainly in degranulated areas of B-cells.
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  • 102
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    Cell & tissue research 209 (1980), S. 187-200 
    ISSN: 1432-0878
    Keywords: Pineal organ ; Teleostei ; Syngnathidae ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A comparative ultrastructural study was made of the pineal organ of two related species of the Syngnathidae, the pipefish, Syngnathus acus, and the seahorse, Hippocampus hudsonius. Both species have a relatively small pineal organ, which ultrastructurally shows several similarities with the pineal of other teleosts. In the pineal organ of Syngnathus acus, the photoreceptor cells have well-developed outer segments consisting of about 15 to 60 saccules. The pineal supporting cells are characterized by the presence of numerous myeloid bodies. In Hippocampus hudsonius the pineal outer segments are scarce and poorly developed; generally, they show not more than 20 saccules. The apical parts of the supporting cells contain dense-core vesicles with a diameter varying from 100 to 180 nm. Corresponding vesicles were not observed in the pineal organ of Syngnathus acus. Arguments are presented for the suggestion that the pineal organ of the Syngnathidae, in spite of its relatively small size, still has a functional significance. In Syngnathus acus, light reaching the pineal organ directly might have a regulating effect on pineal activity. In Hippocampus hudsonius, however, light acting directly on the pineal organ appears to have minor effect on pineal function.
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  • 103
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    Cell & tissue research 209 (1980), S. 329-343 
    ISSN: 1432-0878
    Keywords: Stomach ; Innervation ; Myenteric plexus ; Guinea-pig ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The ultrastructure of the vesicle-containing axon profiles in the myenteric ganglia of the guinea-pig stomach was studied in specimens fixed by vascular perfusion and in specimens fixed by immersion in cold fixative after incubation in 5-hydroxydopamine. Three major types of vesicle-containing axon profile were identified in the ganglia: 1. Profiles containing numerous small, mainly spherical vesicles and only limited numbers of large dense-cored vesicles. In perfusion-fixed specimens, the small vesicles in these profiles were all clear. In specimens fixed by immersion after incubation in 5-hydroxydopamine, the profiles could be divided into three types: a) profiles containing small clear vesicles; b) profiles containing small dense-cored vesicles; c) profiles in which the small vesicles contained a peripheral rim of dense material. In these profiles, large vesicles which contained dense cores were rare. The mean diameter of the vesicles in the profiles was also significantly higher than in the profiles containing small clear vesicles. 5. Profiles containing flattened membrane-bounded structures. Reconstructions prepared from serial sections suggested that these structures represented sections through networks of smooth endoplasmic reticulum-like tubules rather than flattened vesicles. 6. Profiles containing many large dense-cored vesicles and few small clear vesicles. In perfusion-fixed specimens, the diameter of the dense-cored vesicles in these profiles was significantly higher than in the type 1 profiles. Type 1 a and type 1 c profiles were much more numerous than either type 2 or type 3 profiles, and type 1 b profiles were few. Synaptic junctions were found in association only with type 1 a profiles. Type 1 a and type 1 b profiles resembled cholinergic and adrenergic axon terminals. The remaining profiles may represent the terminals of different forms of peptidergic axon or of other as yet unidentified types of axon.
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  • 104
    ISSN: 1432-0878
    Keywords: Carbonic anhydrase ; Ultrastructure ; Shell formation ; Ionic regulation ; Lymnaea stagnalis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The ultrastructural localization of carbonic anhydrase (CA) was investigated with the cobalt-bicarbonate method in three epithelia of the pond snail Lymnaea stagnalis. In the epidermis a selective population of “positive cells” was observed. In these cells, CA is confined to the apical and to small parts of the lateral plasma membrane. In cells of the outer mantle epithelium, CA is localized in the lateral and basal parts of the plasma membrane. In cells of the ureter, CA was found apically as well as basally. The localization of CA is discussed in relation to the different functions of the epidermis (electrolyte uptake), mantle (HCO 3 − secretion, calcification) and ureter (electrolyte uptake, acid-base regulation).
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  • 105
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    Cell & tissue research 210 (1980), S. 21-32 
    ISSN: 1432-0878
    Keywords: Eyespots ; Photoreceptor ; Chemoreceptor ; Turbellaria ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The eyespots of Microstomum lineare were studied by electron microscopy, light microscopy, and fluorescence microscopy. Each eyespot consists of two ciliary photoreceptor cells shielded by pigment cells and additional sensory cells. The photoreceptor cells are characterized by a distal intracellular cavity lined with 50–100 interwoven cilia. The other sensory cells are of two ultrastructurally different types, one with long cilia predominating and the other with balloonlike cilia. The pigment cells, which envelop processes of the sensory cells, contain pigment vacuoles varying in size and content and give a bright red fluorescence by the Falck-Hillarp method. The eyespots are suggested to perform a dual function as photoreceptors and chemoreceptors. The evolutionary significance of ciliary photoreceptors in Turbellaria is discussed.
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  • 106
    ISSN: 1432-0878
    Keywords: Myocardial cells ; Ultrastructure ; Differentiation ; T-tubule formation ; Opossum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Cardiac muscle cells in the right ventricle of the postnatal opposum were studied ultrastructurally, with particular attention to the developmental stage of T-tubule formation. Animals ranging from 5.5cm (day 29 of postnatal life) to 41 cm (young adult) in body length were used. Typical T-tubules were first recognized in a few myocardial cells of the 7.5cm (day 43) opossum. T-tubules increased in number as cardiac muscle cells continued to differentiate until 22cm in body length (about 105 days after birth). At this stage of development most of the T-tubules were completely established. The general mode of differentiation and development of cardiac muscle cells appeared essentially the same as in other mammals.
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  • 107
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    Cell & tissue research 210 (1980), S. 155-165 
    ISSN: 1432-0878
    Keywords: Sympathetic ganglion ; Teleost ; Ultrastructure ; Histochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In order to compare the structure of a teleost sympathetic ganglion with those of other vertebrates, light, fluorescence histochemical and electron microscopy were carried out on the coeliac ganglion of the scorpion fish, Myoxocephalus scorpius. In common with studies on other vertebrates, fluorescence histochemistry distinguished two cell types: a) principal neurones which exhibited low levels of specific catecholamine fluorescence and comprise the majority of neurones in the ganglia, and b) smaller intensely fluorescent cells, some of which had processes tens of micrometers long. With the electron microscope, the principal cells were seen to make axodendritic and axosomatic synapses with axons containing mainly 30 nm agranular vesicles at the synaptic site while in other vertebrates usually only one or other synaptic association is present. Both the somata and the processes of intensely fluorescent cells contain 300–600 nm diameter vesicles many of which have electron dense cores. These cells are also innervated by axons containing 30 nm agranular vesicles.
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  • 108
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    Cell & tissue research 210 (1980), S. 249-256 
    ISSN: 1432-0878
    Keywords: Synaptic ribbons ; Pineal organ ; Goldfish ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Synaptic ribbons in the pineal organ of the goldfish were examined electron microscopically with particular attention to their topography. These structures were formed of parallel membranes, which were poorly preserved with OsO4 fixation and could be extracted from thin sections with pronase indicating their proteinaceous nature. Synaptic ribbons were closely apposed to the plasma membrane bordering dendrites of ganglion cells, but were also related to processes of both photoreceptor and supportive cells. Their close proximity to invaginations of the plasma membrane and portions of the endoplasmic reticulum suggest that they are involved in the turnover of cytoplasmic membranes. Tubular and spherical organelles of unknown function are also described.
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  • 109
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    Cell & tissue research 210 (1980), S. 359-369 
    ISSN: 1432-0878
    Keywords: Epidermis ; Acidophilic cell ; Histochemistry ; Ultrastructure ; Salmo trutta L
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Acidophilic cells occur in the epidermis of several species of salmonid fish, although their abundance fluctuates considerably between individuals within the same population and at different times during the life cycle. The histology, histochemistry and ultrastructure of an acidophilic, granular celltype in the epidermis of the brown trout, Salmo trutta L., is described. At the light microscope level this cell type is easily distinguished from the large, mucus-secreting, epidermal goblet cells by its acidophilic, proteinaceous secretion. At the ultrastructural level this secretion consists of membrane-bound granules formed by the very active Golgi region. It is argued that the acidophilic, granular cell is not a transformed blood cell but constitutes a normal epidermal component of the brown trout. Possible roles of this cell in the function(s) of the epidermis are discussed.
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  • 110
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    Cell & tissue research 210 (1980), S. 435-445 
    ISSN: 1432-0878
    Keywords: Ultrastructure ; Mechanoreceptor ; Skin ; Reptile
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The histological and ultrastructural organisation of the epidermal sensory organs in Amphibolurus barbatus has been described with respect to their position and possible functions. The sensory organs, located at the scale's edge, are most numerous in scales of the dorsal surface of the head. Most other scales of the body surface have two receptors located laterally to the spine or keel of the scale. In the imbricate scales of the ventral body region, the receptors lie just beneath the reinforced scale lip. Scanning electron microscopy has revealed the surface of the organ to be a crater lacking any surface projections. These sensory organs have a dermal papilla consisting of a nerve plexus and loose connective tissue. The nerve fibres arising from the plexus, pass to the epidermal columnar cells, where some form nerve terminals at the base of the cells, while others pass between them to form nerve terminals embedded in a superficial layer of cuboidal cells. The superficial terminals are held against the overlying α keratin by masses of tonofilaments. The β keratin is thickened to form a collar around the periphery of the organ but is only about 0.5 μm thick immediately above it. Mechanical deformation of the scale's spine or reinforced scale lip may initiate stimulation of the nerve terminals described.
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  • 111
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    Cell & tissue research 211 (1980), S. 31-40 
    ISSN: 1432-0878
    Keywords: Nervous system ; Turbellaria ; Fluorescence histochemistry ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The nervous system (NS) of Microstomum lineare (Turbellaria, Macrostomida) was studied by electron and light microscopy, combined with fluorescence histochemistry (Falck-Hillarp method for biogenic monoamines). The NS is primitively organized, with a bilobed brain, two lateral nerve cords lacking commissures, and peripheral nerve cells scattered along the nerve cords. The stomatogastric NS, with a pharyngeal nerve ring, is joined to the central NS by a pair of connective ganglia. A green fluorescence in all parts of the NS indicates catecholaminergic neurons as the dominant neuron type. Ultrastructurally, two types of neurons were identified on the basis of their vesicle content: 1. Aminergic (catecholaminergic) neurons containing densecore vesicles of varying electron-density and size, i.e., small dense-core vesicles (diameter 50–100 nm), vesicles with a highly electron-dense core (60–140 nm), and vesicles with an eccentric dense-core. 2. Presumed peptidergic neuro-secretory neurons containing large granular vesicles (diameter about 200 nm) in the stomatogastric NS and peripheral parts of the central NS. In light microscopy, paraldehyde-thionin stained neurons were observed in the same areas.
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  • 112
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    Cell & tissue research 211 (1980), S. 151-161 
    ISSN: 1432-0878
    Keywords: Pinealocytes ; Pig ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Pinealocytes of female pigs were studied electron-microscopically and compared with those of other mammals. A prominent Golgi apparatus forming dense-cored vesicles was widely dispersed in the cytoplasm of the cell body. A very characteristic feature of the pig pinealocytes was the presence of membrane-bounded bodies showing wide variations in internal structure. Possible roles of the dense-cored vesicles and membrane-bounded bodies in secretory processes of pinealocytes are discussed.
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  • 113
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    Cell & tissue research 211 (1980), S. 391-403 
    ISSN: 1432-0878
    Keywords: Ocelli ; Dinopid spider ; Ultrastructure ; Optics ; Evolution of nocturnal eye
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The posterior median (pm) eyes of the dinopid spider Menneus unifasciatus L. Koch are described and compared with the pm eyes of Dinopis, which are highly specialised for night vision. The lenses of Menneus have F-numbers of 0.72 compared to 0.58 in Dinopis, the distance between receptors is ca. 4.0 μm compared to 20–22 μm for Dinopis, and image quality is matched to receptor spacing. The lens of Menneus is simple, while that of Dinopis comprises two components of different refractive indices (Blest and Land 1977). Receptive segments of the pm eyes of Dinopis are hexagonal in transverse section and those of adjacent cells are tightly contiguous, allowing the possibility of both optical and electrical coupling (Blest 1978). Receptive segments of Menneus are separated from each other by glial processes containing little pigment, and each segment possesses two rhabdomeres on opposite faces of the cell. Rhabdomere volumes undergo a daily cycle similar to that described for Dinopis, but of relatively minor extent. It is shown that the pm eye of Dinopis could have evolved from that of Menneus by a simple series of transformations, and that a gain of two logarithmic units of sensitivity can be attributed to changes in optical design alone.
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  • 114
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    Cell & tissue research 211 (1980), S. 449-463 
    ISSN: 1432-0878
    Keywords: Ependyma ; Choroid plexus ; Leptomeninges ; Ultrastructure ; Bullfrog ; Tela choroidea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The lateral areas of the rhombencephalic tela of the bullfrog contain long, irregular islands of ependymal cells that are similar in fine structure to the epithelium of the rhombencephalic choroid plexus. These cells are characterized by apical microvilli, numerous mitochondria and pinocytotic vesicles, and basal infoldings of the plasma membrane. Dorsally a basal lamina and varying amounts of collagen occur. The pia mater associated with this ependyma includes two cell types. Fibroblast-like, loosely arranged cells without organized junctions line the subarachnoid space. The most abundant cells of the pia in this area, however, contain numerous intermediate filaments and frequent desmosomes. Caveolae lie along their plasma membranes. Closely organized sheets of similar filament-containing cells are also seen in the arachnoid mater of this animal. These findings demonstrate ependymal cells in the lateral areas of the rhombencephalic tela of the bullfrog that have the essential features of choroid plexus epithelium, with ultrastructural characteristics that suggest transport function. They are, however, usually separated from neighboring, nonfenestrated vessels by several layers of leptomeningeal cells joined by desmosomes. The relationship between structure and function of these cells is enigmatic.
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  • 115
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    Cell & tissue research 212 (1980), S. 475-495 
    ISSN: 1432-0878
    Keywords: Distal tubule ; Connecting tubule ; Collecting duct ; Ultrastructure ; Desert rodent (Psammomys obesus)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The transition from the nephron to the collecting duct is formed by three tubular segments (convoluted part of the distal tubule, connecting tubule, cortical collecting duct), which in the desert rodent, Psammomys obesus, transform gradually from one segment to the next, due to intermingling of their different cell types. The convoluted part of the distal tubule (DTC) starts abruptly, shortly beyond the macula densa and initially is homogeneously composed of characteristic DTC-cells. Subsequently, the DTC-cells intermingle with intercalated cells. The first appearance of the connecting-tubule cell, which gradually replaces the DTC-cell, is regarded as the beginning of the connecting tubule. The major portion of the connecting tubule is lined by connecting-tubule cells and intercalated cells. The first appearance of the principal cell between them defines the beginning of the cortical collecting duct, which in the medullary ray is lined by principal and intercalated cells only. Each cell type is described in detail and discussed in relation to the assumed function of the tubular segments. Interspecies differences in the cellular composition of the transitional zone from the nephron to the collecting duct are discussed in relation to the different organization of the collecting duct system.
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  • 116
    ISSN: 1432-0878
    Keywords: Anterior pituitary ; Microtubules ; Paracrystalline aggregates ; Ultrastructure ; Chinchilla
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Unusual paracrystalline aggregates of microtubules which have not been described in any other mammalian species were observed in cultured anterior pituitary cells of normal chinchillas as well as in situ in the pituitary glands of these animals. These aggregates appeared as regularly arranged tubular structures in the longitudinal plane, and as a checkerboard pattern of closely and regularly packed microtubules when examined in transverse section. Supplementation with vinblastine, colcemide or colchicine in the culture medium did not change these structures morphologically. Each unit of tubules consisted of an outer wall or parellelogram profile and an inner wall composed of a single hexagonal doublet or in a figure “8” form. The outer wall of the parallelogram was 35×28 nm in length for both sides, while the diagonal of the inner wall was 18×28 nm. These paracrystalline aggregates of microtubules in the chinchilla pituitary cells are morphologically distinct from the paracrystalline assembly of cytoplasmic microtubules induced by vinblastine or other alkaloids. The function and significance of these paracrystalline aggregates in anterior pituitary cells of the chinchilla are uncertain.
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  • 117
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    Cell & tissue research 222 (1982), S. 41-51 
    ISSN: 1432-0878
    Keywords: Ultrastructure ; Interstitial cell ; Myenteric plexus ; Colon ; Rabbit
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The interstitial cells associated with the myenteric plexus of the rabbit colon were studied by scanning and transmission electron microscopy. It was demonstrated that the interstitial cells were stellate or fusiform in shape and located over the ganglia, over nerve bundles and between muscle cells. They were characterized by many slender processes, and resemble fibroblasts. No basal lamina was observed between the interstitial cells and muscle cells. It was concluded that structural features of the interstitial cells are distinctly different from those of neurons, Schwann cells, or of smooth muscle cells, while they show clear similarities to those of fibroblasts. By scanning electron microscopy the shapes and the relations of these cells could be demonstrated in great detail.
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  • 118
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    Cell & tissue research 222 (1982), S. 359-378 
    ISSN: 1432-0878
    Keywords: Axon terminals ; Substantia gelatinosa ; Spinal cord ; Noradrenaline ; Ultrastructure ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The noradrenergic terminals in the substantia gelatinosa of the dorsal horn of the cervical spinal cord of the rat were investigated by means of the histofluorescence technique and electron-microscopic cytochemistry using the glyoxylic acid-KMnO4 fixation technique. In accordance with the topographical distribution of fluorescent catecholaminergic fibers, noradrenergic terminals containing small granular vesicles were frequently observed electron microscopically in the outer layer of the substantia gelatinosa. These terminals were most frequently found to appose without showing typical synaptic features, small-caliber dendrites, spine apparatus, and rarely, large caliber dendrites. Only in a few cases, the noradrenergic terminals exhibited typical synaptic contacts with dendritic elements of small size. In addition, noradrenergic terminals apposed non-noradrenergic terminals containing small agranular vesicles. In rats bearing surgical lesions of the dorsal roots, no noradrenergic terminal were found in contact with the degenerated axon terminals in the substantia gelatinosa. These findings suggest that the noradrenergic afferents to the substantia gelatinosa may exert their influence on sensory transmission via dorsal horn cells.
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  • 119
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    Cell & tissue research 222 (1982), S. 695-698 
    ISSN: 1432-0878
    Keywords: Pineal gland ; Ultrastructure ; Dense-core vesicles ; Cat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Dense-core vesicles, 90–200 nm in diameter, were found in pinealocytes of the cat. They were present both in perikarya and cell processes.
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  • 120
    ISSN: 1432-0878
    Keywords: Immunoelectron microscopy ; Preoptic nucleus ; Goldfish ; Magnocellular neuroendocrine cells ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary We describe the ultrastructural localization of isotocin, vasotocin and neurophysin in the magnocellular preoptic nucleus of the goldfish. With the aid of immunocytochemical techniques, we see staining both in classical neurosecretory granules and in diffuse agranular form throughout somata and processes. Signs of cellular and synaptic interactions between chemically identified neurons include axon terminals which contain vasotocin immunoreactivity and membrane specializations (puncta adhaerentia) between adjacent somata. Our investigations provide an anatomical basis for neuroendocrine and neurotransmitter-like functions of peptidergic neurons in the teleost preoptic nucleus.
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  • 121
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    Cell & tissue research 224 (1982), S. 195-206 
    ISSN: 1432-0878
    Keywords: Pinealocytes ; Nucleolus ; Rat ; Ultrastructure ; Twenty-four-hour changes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary An ultrastructural and morphometric analysis was made of the nucleolar components in pinealocytes of 40 male Fischer rats sampled at eight times in an LD 12∶12 photoperiod cycle. Comparisons of results from the eight times showed variation in estimated mean volume of the granular component of ±29%, and of the fibrillar component ±11%, in relation to daily means. Peaks in mean volume of total nucleolus and its granular component occurred at 1 h of light. Near maximal and minimal mean volumes of the fibrillar component both occurred during both light and dark. Fibrillar centers (nucleolar organizer regions) of different sizes were found at all sampling times. It is concluded that temporal patterns in 24-h changes in the nucleolar components are most prominent in the granular component, and are more complex than suggested by changes in total nucleolar size or mean dimensions, and than represented by a simple biphasic circadian rhythm. Examples of different stages in the migration of the granular component, and of possible sites of nucleo-cytoplasmic transfer of nucleolar material, are described.
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  • 122
    ISSN: 1432-0878
    Keywords: GALT (gut-associated lymphoid tissue) ; Urodeles ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Résumé L'ultrastructure du tissu lymphoïde associé au tube digestif (GALT) a été étudiée chez l'amphibien urodèle, Pleurodeles waltlii. Les follicules lymphoïdes se présentent comme de vrais infiltrés entre les éléments conjonctifs de la muqueuse. Ils se trouvent principalement constitués par des plasmocytes mûrs et en développement, des macrophages et des granulocytes. Les cellules lymphoïdes migratrices provoquent une invasion massive de l'épithelium intestinal qui présente des modifications notables comme la disparition de la membrane basale et une diminution du nombre de cellules muqueuses. D'après son organisation et ses composants cellulaires, le GALT de P. waltlii semble représenter un précurseur phylogénétique primitif de la “barrière immunologique intestinale” des mammifères.
    Notes: Summary The ultrastructure of gut-associated lymphoid tissue (GALT) has been studied in the salamander, Pleurodeles waltlii. Lymphoid accumulations appear as true infiltrates scattered throughout the lamina propria cell elements. The most important components of these infiltrates are small and medium sized lymphocytes, and, in lesser amounts, developing and mature plasma cells, macrophages and granulocytes. Migrating lymphoid cells massively invade the intestinal epithelium inducing noticeable modifications, such as the disappearance of the basement membrane and decreased numbers of mucous cells. Thus, in its organization and cell composition, the GALT of P. waltlii appears to represent a primitive phylogenetic precursor of the mammalian “intestinalimmunologic ” barrier.
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  • 123
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    Cell & tissue research 225 (1982), S. 111-127 
    ISSN: 1432-0878
    Keywords: Loops of Henle ; Thin limbs ; Kidney (hamster) ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In the kidney of the Syrian hamster the descending thin limbs of both the short and long loops of Henle are not spatially separated from each other and descend between the vascular bundles. Ultrastructurally, five different epithelial types are distinguished in the thin limbs of the short and long loops of Henle. Short loops possess only a descending thin limb with a simply organized epithelium (type 1). Long loops comprise an upper and a lower part of the descending thin limb and the ascending thin limb. The upper part of the long descending thin limb is equipped with a complex and highly interdigitating epithelium with shallow junctions (type 2), which gradually transforms into the simple noninterdigitating type-3 epithelium of the lower part. In a minor portion of long descending thin limbs, however, the upper part begins with an even more complexly organized epithelium (type 2a) than type 2. Type-2a epithelium is conspicuously thicker and possesses a more elaborate mode of cellular interdigitation. Along the descent of this tubular part through the inner stripe of the outer medulla, type-2a epithelium transforms into type-2 epithelium. It is suggested that the long descending thin limbs, which start with type-2a epithelium, belong to the longest loops. The type-4 epithelium of the ascending thin limbs is characterized by flat and extensively interdigitating cells with shallow junctions. The unique pattern of the type-2 a epithelium favors the assumption that solute secretion essentially contributes to the increase in concentration of tubular fluid in long descending thin limbs.
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  • 124
    ISSN: 1432-0878
    Keywords: Opiate peptides ; Pituitary ; Pro-opiomelanocortin cells ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The effects of acute injections of synthetic opiate peptides into the lateral cerebral ventricle of young adult male rats on cells of the intermediate lobe of the pituitary were studied. Met-enkephalin (100/μg) injected into anesthetized rats, or 20 μg beta-endorphin administered via a previously implanted cannula to unanesthetized animals, will lead to cell degranulation and often to expanded Golgi zones and prominent regions of rough endoplasmic reticulum in secretory cells when tissue is fixed 45–60 min after peptide administration. Treatment of animals with the opiate antagonist naloxone hydrochloride prior to enkephalin injection appeared to prevent the cellular changes elicited with peptide alone. Observations suggest that opiate peptides administered to the cerebrospinal fluid may stimulate release of pro-opiomelanocortin-peptide from pituitary cells.
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    Cell & tissue research 226 (1982), S. 493-510 
    ISSN: 1432-0878
    Keywords: Ovarian cords ; Surface epithelium ; Fetal germ cells ; Mesonephros ; Ultrastructure ; SEM
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The ovarian surface and associated germ cells have been studied in human fetuses from 12 weeks of age until near term, using light, TEM and SEM techniques. The surface epithelium and related cords proliferate extensively, especially at midterm. The cords in the ovarian cortex appear to be linked with ingrowths from the surface epithelium, and both structures have a common basal lamina. Germ cells are always interspersed among the somatic cells of the surface epithelium and associated cords. These results indicate that both the proliferating cords and surface epithelium may contribute to the formation of early follicles. Furthermore, the occurrence, of elements having some of the features of primitive steroidogenic cells in the regions of cordsurface epithelium continuity, suggests that both structures (surface epithelium and cords) contribute somatic cells, which in addition to becoming granulosa cells, might also contribute to the provision of primitive interstitial cells. Gonocytes tend to migrate through the developing ovarian tissue towards the surface where they become extruded into the peritoneal cavity. This phenomenon might contribute to the reduction in the number of germ cells at birth and parallels the atretic processes within the ovary.
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  • 126
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    Cell & tissue research 226 (1982), S. 541-554 
    ISSN: 1432-0878
    Keywords: Oral epithelium ; Cell differentiation ; Vitamin A ; Ultrastructure ; Stereology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The ultrastructural changes induced by the topical application of retinol acetate on hamster cheek pouch epithelium were evaluated using stereological analysis. Electron micrographs were prepared of the basal and superficial regions of the nucleated cell layer of the epithelium obtained from 3 treated and 3 control animals and examined at two levels of magnification. A total of 528 micrographs were analyzed using a coherent double lattice test system. Although the mean thickness of the nucleated cell layer did not change significantly after 10 days of treatment with retinol acetate the formation of keratinized squames was completely inhibited. This was paralleled by significant changes in the volume density of a number of organelles in both the basal and superficial strata. Rough endoplasmic reticulum increased significantly whereas filaments, which maintained a constant diameter of approximately 9 nm, keratohyalin granules and membrane-coating granules decreased in both strata. Desmosomes also showed a significant decrease in numerical area density in the treated tissues. In contrast, no changes were observed in the volume density of the Golgi apparatus, free ribosomes or mitochondria in the treated epithelium. It is concluded that this treatment provides an epithelium lacking all features of keratinization and may be a useful model for examining metabolic activities specifically associated with keratinization.
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  • 127
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    Cell & tissue research 226 (1982), S. 565-578 
    ISSN: 1432-0878
    Keywords: Mechanosensory hair ; Chordotonal organ ; Ultrastructure ; Crustacea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In this study we examine the fine structure of mechanosensory hairs in the antennule of crayfish. The sensory hair is a stiff shaft with feather-like filaments. The hair's base is a large expansion of membrane which allows the hair shaft to deflect. The sensory transducing elements are located far from the hair, but are coupled mechanically with the hair shaft by a fine extracellular chorda. The sensory element is a type of scolopidium which consists of a scolopale cell and three sensory cells with a 9 + 0 type ciliary process. This type of scolopidium is characteristic of the chordotonal organ that has no cuticular structure on the surface of the exoskeleton. In this crustacean hair receptor, the deflection of the cuticular hair is transmitted through the chorda to the scolopidium which is a tension-sensitive transducer. The present study reveals that the mechanosensory hair of decapod crustaceans is a chordotonal organ accompanied by a cuticular hair structure. We also discuss comparative aspects of cuticular and subcuticular chordotonal organs in arthropods.
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  • 128
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    Cell & tissue research 227 (1982), S. 129-137 
    ISSN: 1432-0878
    Keywords: Mitochondria ; Ultrastructure ; Microinjection ; ATP ; ADP ; Amoeba proteus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Microinjection of adenine nucleotides and substrates into the cytoplasm of Amoeba proteus followed by EM examination has been used in an attempt to relate alterations in mitochondrial morphology with functional changes. Contracted mitochondria with dark matrix and wide cristae (Type I), and expanded mitochondria with light matrix and narrower cristae (Type II) coexist in normal active amoebae, but their numbers can be varied according to different cell activity states. Following injection of ATP, the mitochondria of the amoebae showed a time-dependent movement towards a predominately Type II form, whilst injections of ADP produced predominately the Type I form. Injection of succinate or deionised water, even in large amounts, had little effect on the numbers of Type I or Type II forms. The change induced by ATP was of long duration; that induced by ADP was influenced by both concentration injected and the cell's substrate levels. With 3 mM solutions of ADP the mitochondrial population was primarily of Type I organelles; higher ADP concentrations or the simultaneous injection of succinate, however, resulted in a switch with time to increased proportions of Type II mitochondria. The results extend the findings of previous in vivo and in vitro mitochondrial studies and are discussed in the light of these.
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  • 129
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    Cell & tissue research 227 (1982), S. 139-151 
    ISSN: 1432-0878
    Keywords: Migration ; Primordial germ cell ; Teleost ; Oryzias latipes ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The primordial germ cells (PGCs) of Oryzias latipes in migration to the gonadal anlage have been investigated by light and electron microscopy. The ultrastructure of the PGCs, which occur in the subendodermal space on the syncytial periblast, differ conspicuously from that of the surrounding endodermal cells. After the PGCs move to the cavity between lateral plate and ectoderm, they are taken into the somatomesodermal layer and transferred to the dorsal mesentery where they form gonadal anlage with mesodermal cells. During their translocation to the dorsal mesentery through the somatic mesoderm, apparently without formation of pseudopods, the PGCs are completely surrounded by mesodermal cells. Since these conditions seem unfavorable to the active translocation of the PGCs to the dorsal mesentery, it is more likely that the PGCs are transferred passively by the morphogenic activity of the lateral-plate mesoderm. Counts of the number of the PGCs revealed that they are mitotically dormant during the migratory period. After the completion of the migration, they regain their proliferative activity. The PGCs in the female proliferate more actively than those in the male, which provides the first morphological indication of sex differentiation in this species of fish.
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    Cell & tissue research 227 (1982), S. 201-211 
    ISSN: 1432-0878
    Keywords: Optic systems ; Axonal growth ; Myelination ; Ultrastructure ; Teleosts
    Source: Springer Online Journal Archives 1860-2000
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    Notes: Summary The early differentiation of the optic pathway of the trout was studied by means of autoradiography, silver impregnation and electron microscopy. Ingrowth of optic nerve fibers into the optic tectum was consistently shown by tracer application and Golgi studies to occur at stage 28, about one week before hatching. Fibers being arranged in discrete bundles were rapidly growing through the longitudinal axis of tectum and at stage 33 reached its posterior end. Cross sections of these fiber bundles at different positions revealed myelin ensheatment to be initiated at the end of stage 34 at the anterior pole of the tectum. Since in the optic nerve of the trout the onset of myelination occurred even earlier (stage 33), it is assumed that this differentiation process follows a rostro-caudal gradient during development of the optic pathway.
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    Cell & tissue research 227 (1982), S. 371-386 
    ISSN: 1432-0878
    Keywords: Tentacle sensory input system ; Neuroendocrine control of growth ; Ultrastructure ; L. stagnalis
    Source: Springer Online Journal Archives 1860-2000
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    Notes: Summary Several environmental factors influence the growth of the basommatophoran freshwater snail Lymnaea stagnalis. Growth is hormonally controlled by 4 cerebral clusters of ca 50–75 peptidergic, neuroendocrine Light Green Cells (LGC). The present light, transmission, and scanning electron microscopic study shows that the LGC are synaptically contacted by a tentacle sensory system (TSS). The TSS consists of 2 types of primary sensory neurone, viz. ca 150 S1-cells and ca 50–100 S2-cells. A S1-cell has a non-ciliated dendrite and an axon branch that synaptically contacts the soma of a S2-cell. A S2-cell has a branching, ciliated dendrite. Probably, S1- and S2-cells have different sensory modalities and can integrate sensory information by intersensory interaction. The S2-axons run through the tentacular nerves, the cerebral ganglia, and the intercerebral commissure. In each ganglion S2-axons branch and form synaptic contacts on the axons and somata of the LGC and on glial cells that surround the LGC. In an LGC-cluster, 1–3 LGC-somata are particularly strongly innervated. Probably, the TSS is involved in the environmental control of growth in L. stagnalis.
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    Cell & tissue research 213 (1980), S. 475-482 
    ISSN: 1432-0878
    Keywords: Vaginal epithelium ; Prenatal estrogen treatment ; Mucification ; Ultrastructure ; Mouse
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary After transplacental treatment of mice with estrogens, a heavy mucification was found in the fornices of the vaginae of the offsping. The resulting mucified stratified epithelium is described by light and electron microscopy. It consists of two different cell types: cells forming mucus on top of cells forming tonofilaments. Mucus formation is usually attributed to treatment with progestagens, or with with estrogens combined with vitamin A. Estrogen treatment per se has been shown to be responsible for tonofilament formation. Our unexpected findings are discussed against the background of different theories of development of vaginal epithelium as well as their possible interpretation as beginning of adenosis.
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    Cell & tissue research 221 (1982), S. 551-581 
    ISSN: 1432-0878
    Keywords: Non-adrenergic, non-cholinergic nerves ; Cholinergic nerves ; Ultrastructure ; Nervous transmission
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In order to test the premise that non-adrenergic, non-cholinergic (NANC) autonomic nerves have a distinctive ultrastructural appearance, clearly different from that of cholinergic nerves, a detailed quantitative ultrastructural analysis has been made of the non-adrenergic innervation of 15 tissues thought from pharmacological evidence to be innervated by NANC nerves (rat and rabbit anococcygeus muscles; rabbit hepatic portal vein; extrinsically denervated toad lung); cholinergic nerves (atria of rat, rabbit, guinea-pig and toad); or both (guinea-pig cervical and thoracic trachealis muscle; rabbit rectococcygeus muscle; urinary bladder of rat, rabbit, guinea-pig and toad) in addition to their adrenergic supply. Following fixation with a modified chromaffin procedure allowing identification of adrenergic nerves, large, randomly selected samples of non-adrenergic nerve profiles from each tissue were analysed with respect to numbers, relative proportions, and size frequency distributions of different vesicle classes within the profiles. The neuromuscular relationships within each tissue were also analysed. On the basis of these analyses, it is clear that there are no consistent ultrastructural differences between cholinergic and NANC autonomic nerves: neither proportions nor sizes of the vesicles provide any clue as to the transmitter used by a particular nerve. The great majority of nerve profiles, whether cholinergic or NANC, contain predominantly small clear “synaptic” vesicles. Large filled “peptidergic” vesicles are no more common in most NANC nerves than in most cholinergic ones. It is concluded, on ultrastructural grounds, that the primary transmitter in these NANC autonomie nerves is most likely to be stored in and released from the small clear vesicles.
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    Cell & tissue research 221 (1982), S. 607-615 
    ISSN: 1432-0878
    Keywords: Hemocytes ; Drosophila ; Ultrastructure ; Phagocytosis
    Source: Springer Online Journal Archives 1860-2000
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    Notes: Summary Hemocytes of Drosophila melanogaster and Drosophila yakuba larvae have been defined in terms of their ultrastructure and functions in “coagulation”, wound healing, encapsulation, phenol-oxydase activity, and phagocytosis. The position of these cells among the classical hemocyte types of insects is determined. We distinguish two plasmatocyte types (macrophage plasmatocytes and lamellocytes) which do not seem to belong to the same lineage, and oenocytoids which are the crystal cells of the literature.
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  • 135
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    Keywords: Melanophores ; Periodic albinism ; Ultrastructure ; Physiology ; Xenopus laevis, tadpoles
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    Notes: Summary Pigment of tail-fin melanophores in periodic albino Xenopus laevis tadpoles is dispersed in response to darkness and to α-MSH in a manner similar to wild-type melanophores. However, periodic albino tadpoles lack the response to different background conditions and the melatonin-induced aggregation in darkness. The tyrosinase activity in cells of the latter type tadpoles is weak compared to the wild-type cells. Ultrastructural examination of melanophores from periodic albino mutants and cells from wild-type tadpoles shows similar organelles at corresponding sites. A morphological difference can be observed in the fine structure of the melanosomes, which in albinos resembles an earlier stage of development. It is postulated that periodic albino Xenopus laevis possess the cellular mechanism to disperse pigment in the melanophores, but that under physiological conditions the release of α-MSH appears to be absent or scarce.
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    Cell & tissue research 222 (1982), S. 143-152 
    ISSN: 1432-0878
    Keywords: Sertoli cells ; Seminiferous tubules ; Irradiated rats ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The ultrastructure of the seminiferous tubules was studied in rats that had been subjected to whole body irradiation on the 19th day of gestation. The seminiferous tubules from 3 months-old irradiated animals are devoid of germ cells and contain only Sertoli cells. Compared with controls of the same age, the seminiferous tubule basal membrane is thickened and multilayered and several alterations are observed in the Sertoli cells. The most characteristic of these alterations are: (a) an abnormal number of nuclear heterochromatin clumps, (b) the presence of numerous cytoplasmic vacuoles and various sized lipid droplets, (c) elaborate interdigitations and junctions between adjacent cells, and (d) the presence of anomalous ectoplasmic specializations disposed perpendicularly to the Sertoli cell membrane.
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    Cell & tissue research 222 (1982), S. 531-546 
    ISSN: 1432-0878
    Keywords: Pineal gland ; Ultrastructure ; Chinese hamster (Cricetulus griseus)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The ultrastructure of the pineal parenchymal cells, pinealocytes and glia-like cells, of the Chinese hamster (Cricetulus griseus) is described. Pinealocyte nuclei contain spindle-shaped inclusions consisting of bundles of closely packed parallel filaments of about 8 nm in thickness. Mitochondria contain lamellar and tubular cristae. Smaller and larger mitochondria are usually localized separately in individual pinealocytes. Flattened mitochondria contain two to four closely arranged, unusual lamellar cristae, which lie parallel to the surface of the mitochondria. These cristae exhibit particular structures. Rough endoplasmic reticulum is frequently found in close association with such flattened mitochondria. Tubular expansions of the outer membrane of mitochondria occur frequently. The pinealocyte cytoplasm contains stacks of flattened cisternae, which are continuous with both smooth and rough endoplasmic reticulum. Pinealocyte cell bodies and processes contain granulated vesicles. Although larger granulated vesicles measuring up to 300 nm in diameter occasionally occur, smaller granulated vesicles (about 100 nm in diameter) and larger ones are usually localized separately in individual cells. Vacuoles containing flocculent material are found in groups in close association with rough endoplasmic reticulum. Glia-like cells with darker cytoplasm and nuclei lie surrounding pericapillary or intercellular spaces. The glia-like cells are characterized by the presence of abundant lipid droplets and occasional pigment granules. Bundles of filaments run parallel to the long axis of the processes of the glia-like cells.
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    Cell & tissue research 222 (1982), S. 479-491 
    ISSN: 1432-0878
    Keywords: Gastrin cell ; Secretory granules ; Secretory process ; Peptide hormones ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The ultrastructure of gastrin cells in the rat antrum was analyzed with standardized and quantitative planimetric methods. Resting and active cells were compared. The gastrin cells were activated by removal of the acidproducing part of the stomach (fundectomy). As a result the serum gastrin concentrations were greatly elevated. Compared with gastrin cells in fasted control rats the gastrin cells in fundectomized rats were increased in number, contained fewer cytoplasmic granules, increased amount of endoplasmic reticulum, and an enlarged Golgi area. Generally, the secretory granules of the gastrin cell displayed a wide range of electron density from highly electron-dense to electron-lucent. They exhibited certain characteristic features: 1) Electron-dense granules made up a greater proportion of the total granule population in active gastrin cells than in resting cells. 2) Electron-dense granules were more frequent near the Golgi stacks than in the periphery of the cell. 3) Electron-dense granules were smaller in size than the electron-lucent granules; hence, small electron-dense granules probably represent young granules (progranules), while large, electron-lucent granules represent mature (old) granules. 4) Electron-dense granules invariably displayed a more intense immunoreactivity than electron-lucent granules. The gastrins are generated from a large precursor molecule. The posttranslational processing of this precursor is reflected in the gastrin-component pattern. The gastrin-component pattern in antral extracts of fundectomized and normal fasting rats differed in that the proportion of the gastrin-4-like component was reduced, whereas the gastrin-34-like component was increased in the fundectomized rats. The results suggest a greater proportion of small gastrin components in the mature granules than in the newly formed ones, presumably due to more extensive conversion of larger forms into smaller forms with a longer granule half-life. As a result gastrin-17-and gastrin-34-like components make up a larger proportion of total gastrin in active gastrin cells than in resting gastrin cells.
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    Cell & tissue research 223 (1982), S. 201-215 
    ISSN: 1432-0878
    Keywords: Liver ; Endothelial cells ; Primary culture ; Ultrastructure ; Adsorptive endocytosis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A new isolation and purification procedure for endothelial cells of the rat liver and the conditions for large scale survival of these cells in maintenance culture are reported. Cells isolated by this new method and cultured with homologous rat serum on a collagen matrix show the restoration of several ultrastructural characteristics typical of rat liver endothelial cells in situ, including the broad cytoplasmic extensions that contain the sieve plates. These fenestrated cytoplasmic projections, which cover the liver sinusoids in vivo, are well preserved and are reformed in a manner reminiscent of the situation in situ. Reformation of specific membrane receptors is indicated by the reappearance of the capacity to take up horseradish peroxidase by adsorptive endocytosis, a characteristic that is lost during the cell isolation procedure. From the results obtained in this study, maintenance culture of rat liver endothelial cells seems to be a promising system for studying the regulation of pore size of the fenestrated sieve plates by alcohol and certain hormones, for studying the interaction of endothelial cells with other liver cells and tumor cells, and for studying the mechanisms of adsorptive endocytosis.
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  • 140
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    Keywords: Spleen (rat) ; Ultrastructure ; Periarteriolar lymphatic sheath ; Interdigitating cells
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary To obtain more information concerning the origin of interdigitating cells, the postnatal development and morphology of the periarteriolar lymphatic sheath in splenic white pulp of rats was investigated by light- and electron-microscopy. Special attention was paid to the ontogeny of interdigitating cells. The spleens of the animals were studied in the age range from 1 h to 28 days after birth. The splenic white pulp of neonatal rats consists only of a few reticuloblasts, which are concentrically arranged around central arterioles. After 21 h an increase in promonocytes and monocytes was noted. Between the fifth and seventh postnatal day monocytogenic cells with a light and almost translucent cytoplasm appear, which display long cytoplasmic projections between the adjacent cells. Neighbouring lymphocytes often insert finger-like processes into the invaginated cellular membrane of these transitional forms. This intimate cellular contact is supported by zonulae occludentes. These cells represent transitional forms between monocytes and interdigitating cells. From seven days of age onwards typical interdigitating cells were present as in adult animals. After the differentiation into an inner and outer periarteriolar lymphatic sheath, the T-cell-dependent area of splenic white pulp has attained its adult appearance and further changes are not to be expected. On the basis of these findings, it is highly probable that interdigitating cells develop via transformation of monocytes.
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    Cell & tissue research 223 (1982), S. 349-367 
    ISSN: 1432-0878
    Keywords: Teleost scales ; Ultrastructure ; Rapid freeze-fixation ; Mineralization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Resumé Des précisions concernant les aspects ultrastructuraux des dépôts minéraux dans les écailles deCarassius auratus ont été obtenues grâce à l'utilisation de la congélation ultra-rapide suivie d'une cryosubstitution en milieu anhydre. Ces données sont comparées à celles fournies par les méthodes usuelles utilisant des fixateurs aqueux. La couche externe des écailles comprend des fibres collagènes disposées sans ordre apparent. Les dépôts minéraux se produisent surtout dans la substance interfibrillaire où des granules denses semblent représenter des sites actifs au cours de la minéralisation apparentée au type sphéritique. La plaque basale comporte deux catégories de fibres collagènes. Les unes, les plus nombreuses, de plus fort diamètre, sont organisées en lamelles formant une structure en contre-plaqué; les autres appelées “fibres TC”, orientées de la base de l'écaille vers la zone superficielle, jouent un rôle important dans les premières phases de la minéralisation de type inotropique dans cette partie de l'écaille. Dans les deux couches de l'écaille, la phase minérale est surtout trouvée dans la substance interfibrillaire. De ce fait, les écailles élasmoides des Téleostéens peuvent être distinguées des autres écailles dermiques connues de Vertébrés inférieurs.
    Notes: Summary New data on the ultrastructural features of the elasmoid scales ofCarassius auratus have been obtained by use of rapid freezing with subsequent freeze-substitution in anhydrous solvents. These are compared with the results obtained using conventional aqueous fixatives. The external layer of the scales is composed of randomly oriented collagen fibres. In the first stages of mineralization, mineral deposits are located in the interfibrillary substance where dense granules appear to be active sites of mineralization. Spheritic mineralization occurs in this layer. The fibrillary plate is composed of two kinds of collagen fibres. Most of them are organized in lamellae forming the “plywood-like structure”. They are thicker than the so-called “TC fibres”, which are oriented from the basal part towards the superficial layer. These TC fibres are involved in the first stages of mineral deposition in the fibrillary plate where inotropic mineralization occurs. The mineral phase is almost always located in the interfibrillary matrix in both layers of the elasmoid scale. In this respect, teleost scales differ from those described so far in other lower vertebrates.
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    Cell & tissue research 223 (1982), S. 431-443 
    ISSN: 1432-0878
    Keywords: Eye ; Ultrastructure ; Gambusia affinis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The ultrastructure of the tissue components of the eye ofGambusia affinis, excluding the sensory cells, is described. The cornea consists of two different sections of collagenous layers of different density. The choroid includes an argentea composed ofα- andβ-melanophores, lipopterinophores and a choriocapillaris associated with the rete mirabile of the choroid body. Bruch's membrane, underlying the retinal pigment layer, can develop complex associations with fibroblasts delimiting the choriocapillaris. The outer section (stroma) of the iris includes several cell types that are not found in the inner or vitread section. In adultGambusia the lens capsule is well developed, but in twoweek-oldSarotherodon larvae the lens epithelium is covered only by a glycocalyx.
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  • 143
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    Keywords: Microtubules ; Cattle ; Mammary gland ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Ultrastructural examination of milk secretory cells from lactating bovine mammary gland revealed presence of numerous microtubules in the apical and paranuclear cytoplasm, particularly in the vicinity of Golgi components. Most microtubules were oriented perpendicular to the apical plasma membrane and appeared to form a framework around Golgi dictyosomal elements and secretory vesicles. In comparison, non-secretory cells obtained from involuting glands displayed few microtubules and these were randomly located throughout the cytoplasm with no particular orientation.
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    Cell & tissue research 224 (1982), S. 449-454 
    ISSN: 1432-0878
    Keywords: Gallbladder epithelium ; Brush border ; Apocrine secretion ; Ultrastructure ; Teleosts
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The free surface of the epithelial cells in the gallbladder of the rainbow trout (a predatory fish) and the tench (an omnivorous fish) is characterized by well-developed microvilli. They are irregularly arranged in the tench, but form a true brush border consisting of regularly aligned microvilli in the rainbow trout. In both species membrane-bounded cytoplasmic protrusions, up to 5 μm in diameter, extend from the apical surface. These protrusions are free of granules, secretory vesicles or other organelles; only in the tench some of them contain glycogen. Thus, the previously used terms “apocrine and droplet secretion” are not justified; the epithelial protrusions are now to be regarded as droplets of degenerated cytoplasm. Since they resemble the protrusions reported in fetal and postnatal epithelial cells in the gallbladder of higher vertebrates, interspecific differences are discussed. There is no significant correlation between the seasonal activity of the fish and the abundance or structure of the cytoplasmic protrusions.
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  • 145
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    Keywords: Interdigitating cells ; Thymus ; Birds ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Interdigitating cells (IDC) in the thymus of the spotless starling, Sturnus unicolor, were examined by electron microscopy. They occur principally in the thymic medulla and corticomedullary border. They possess an irregular nucleus and a perinuclear area of cytoplasm, containing most of the membranous organelles, surrounded by a peripheral electron-lucent zone. Clusters of smooth Golgi vesicles and complicated labyrinthine membrane-membrane contacts are the most characteristic cytological features. Birbeck granules are absent. Lymphocytes, plasma cells and even myoid cells can be found embedded in the cytoplasm. Immature elements, intermediate between epithelial-reticular cells and interdigitating cells, are tentatively identified as prointerdigitating cells. The functional significance of IDCs, and their phylogenetic significance in the vertebrate immune system, is discussed.
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    Cell & tissue research 226 (1982), S. 27-35 
    ISSN: 1432-0878
    Keywords: Median eminence ; Ultrastructure ; In vitro systems ; Neurosecretion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Isolated medio-basal hypothalami of adult rats were continuously superfused in a chamber with controllable inputs and outputs, for periods from 30 to 240 min. The median eminence was prepared for transmission electron microscopy under carefully controlled conditions by immersion fixation with osmium tetroxide. The ultrastructure of superfused median eminence was compared with that of directly fixed, non-superfused median eminence. Even after 4h of superfusion, the median eminence displays remarkably well preserved histological and cytological patterns; cytomembranes, cell organelles, intercellular relationships, and extracellular spaces were remarkably similar in superfused and non-superfused tissues. As a consequence of osmium tetroxide fixation, microtubules were not observable. The ultrastructural information obtained from unstimulated rat median eminence superfused in vitro provides a basis for future morphofunctional correlations in the study of neurosecretion.
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  • 147
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    Springer
    Cell & tissue research 226 (1982), S. 51-62 
    ISSN: 1432-0878
    Keywords: Neurohypophysis ; Ultrastructure ; Ageing ; Hormone-containing vesicles
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The neurohypophyses (neural lobes of the pituitary) of young adult (3–6 months) and aged (12–30 months) male rats were studied by means of electron microscopy. Stereomorphometric analyses were performed to determine the size, number and relative volume of the hormone-containing vesicles. The principal observations included: 1) the conspicuous depletion in aged rats of the granular hormone-containing vesicles from the axon terminals and the Herring bodies, with a decrease in the relative volume fraction of the vesicles from 4.8 Vv % in the control animals to 1.1 Vv % in the aged rats; 2) a change in the size-distribution of the hormone vesicles; 3) an increase in the extracellular space around the nerve terminals, axons and capillaries; and 4) lipid accumulations and signs of activation in the pituicytes. The possible physiological significance of the findings is discussed in the light of several regulatory functions known to be altered during the process of ageing.
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  • 148
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    Springer
    Cell & tissue research 226 (1982), S. 237-240 
    ISSN: 1432-0878
    Keywords: Polychaetes ; Neuroendocrine system ; Nerve endings ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In the brain of Ophryotrocha puerilis swollen nerve endings filled with electron-lucent vesicles and aggregates of vesicles were observed. The vesicles do not resemble elementary neurosecretory granules. Tests for biogenic amines were negative; no dense-core vesicles were found. The vesicle type described here cannot be related to any of the types thus far found in nerve cells.
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  • 149
    ISSN: 1432-0878
    Keywords: Exocytosis ; Estrogen ; Ultrastructure ; Canine prostate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Glandular cells in the prostate of the intact, adult dog contain numerous, large secretory granules that are released by exocytosis. Following hypophysectomy or castration, the glandular epithelium atrophies and the secretory granules degenerate and eventually disappear. Pharmacologic doses of estradiol-17β 17-cyclopentylpropionate cause the regressed glandular cells to synthesize a new population of smaller granules that are also released by exocytosis, even though estrogen is known to inhibit fluid secretion by the canine prostate. Thus, the mechanisms involved in prostatic synthesis and exocytosis of secretory granules are independent of those regulating fluid secretion and are operative in the absence of androgen or pituitary hormones.
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  • 150
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    Cell & tissue research 227 (1982), S. 277-290 
    ISSN: 1432-0878
    Keywords: Lymph node (rat) ; Macrophages ; Dendritic reticulum cells ; Immune response to paratyphoid vaccine ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The morphology and kinetics of macrophages and reticulum cells of rat lymph nodes have been studied in relation to the immune response to a second exposure to antigen. During the first 24 h after stimulation monocyte-like exudate macrophages, including some scattered interdigitating cells (IDC), contain granules similar to those present in epidermal Langerhans cells and lymph-borne veiled cells. In this induction phase these macrophages migrate from the marginal sinus into the paracortex and during the migration they gradually transform into IDC. In the proliferation phase the paracortex is mainly populated by transitional macrophages and there are almost no typical IDC present between the lymphoblasts. In the memory phase the relative number of IDC again rapidly increases. During this period in the paracortex there are often typical IDC which contain partially digested necrotic lymphocytes, thus resembling tingible body macrophages (TBM) of the germinal centre in this respect. It is suggested that the newly arrived macrophages induce the lymphoblast reaction, while mature IDC may have an inhibitory function in the memory phase of the immune response. In this phase the phagocytic potential of IDC is clearly shown.
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