ALBERT

All Library Books, journals and Electronic Records Telegrafenberg

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
Filter
  • Nitrogen fixation
  • Springer  (17)
  • American Chemical Society (ACS)
  • National Academy of Sciences
  • 1980-1984
  • 1975-1979  (17)
  • 1979  (17)
Collection
Keywords
Publisher
  • Springer  (17)
  • American Chemical Society (ACS)
  • National Academy of Sciences
Years
  • 1980-1984
  • 1975-1979  (17)
Year
  • 1
    Electronic Resource
    Electronic Resource
    Organic acids and prolonged nitrogenase activity by non-growing, free-living Rhizobium japonicum (1979)
    Springer
    Archives of microbiology 122 (1979), S. 153-159 
    Details
    ISSN: 1432-072X
    Keywords: Rhizobium japonicum ; Nitrogenase activity ; Nitrogen fixation ; Organic acids ; Cell-morphology ; Bacteroids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Rhizobium japonicum 61-A-101 grew and fixed nitrogen more effectively on media containing an organic acid and a pentose sugar than on media containing only one of these carbon sources. Peak specific activities in the range 10–15 nmol C2H4 · h-1 · mg protein-1 were found for these organisms in a spot of growth about 1 cm diameter on agar surfaces exposed to air. Increasing concentrations of the organic acids (succinate or malonate) in a medium containing arabinose resulted in longer lasting activity. The inclusion of a third carbon source, glycerol, gave activity which remained at the maximum from about the 8 to the 18 day after inoculation although no growth of the bacteria occurs during the last 8 or 10 days. At low concentration of organic acid l-arabinose was a much better carbon source for supporting nitrogenase activity of these organisms that the d-form. Both organic acids affected the morphology of the bacteria. Higher concentrations, especially of malonate, gave swollen and distorted cells. When bacteria growing on organic acid-containing agar plates were suspended and plated after appropriate dilution on yeast extract — mannitolglycerol agar there was heterogeneity of colony form, with up to 90% microcolonies after growth on high malonate concentrations. The effects of malonate may be correlated with characteristics of the bacteroid form inside the nodule which contains relatively high concentrations of organic acids, especially malonate.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00411354
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Nitrogenase activity and nitrate respiration inAzospirillum spp. (1979)
    Springer
    Archives of microbiology 121 (1979), S. 141-145 
    Details
    ISSN: 1432-072X
    Keywords: Azospirillum ; Denitrification ; Nitrogen fixation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The interaction between nitrate respiration and nitrogen fixation inAzospirillum lipoferum andA. brasilense was studied. All strains examined were capable of nitrogen fixation (acetylene reduction) under conditions of severe oxygen limitation in the presence of nitrate. A lag phase of about 1 h was observed for both nitrate reduction and nitrogenase activity corresponding to the period of induction of the dissimilatory nitrate reductase. Nitrogenase activity ceased when nitrate was exhausted suggesting that the reduction of nitrate to nitrite, rather than denitrification (the further reduction of nitrite to gas) is coupled to nitrogen fixation. The addition of nitrate to nitrate reductase negative mutants (nr-) ofAzospirillum did not stimulate nitrogenase activity. Under oxygen-limited conditionsA. brasilense andA. lipoferum were also shown to reduce nitrate to ammonia, which accumulated in the medium. Both species, including strains ofA. brasilense which do not possess a dissimilatory nitrite reductase (nir-) were also capable of reducing nitrous oxide to N2.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00689978
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Temperature control of nitrogen fixation in Klebsiella pneumoniae (1979)
    Springer
    Archives of microbiology 123 (1979), S. 259-265 
    Details
    ISSN: 1432-072X
    Keywords: Nitrogen fixation ; Nitrogenase ; Gene expression ; Protein synthesis ; Regulation ; Klebsiella pneumoniae ; Microbial ecology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract At growth temperatures above 37°C, Klebsiella pneumoniae does not grow in a medium containing N2 or NO 3 - as nitrogen sources. However, both the growth in the presence of other nitrogen sources as well as the in vitro nitrogenase activity are not affected at this temperature. The inability to fix N2 at high temperature is due to the failure of the cells to synthesize nitrogenase and other nitrogen fixation (nif) gene encoded proteins. When cells grown under nitrogen fixing conditions at 30°C were shifted to 39°C, there was a rapid decrease of the rate of de novo biosynthesis of nitrogenase (component 1), nitrogenase reductase (component 2), and the nifJ gene product. There was no degradation of nitrogenase at the elevated temperature since preformed enzyme remained stable over a period of at least 3 h at 39°C. Thus, temperature seems to represent a third control system, besides NH 4 + and O2, governing the expression of nif genes of K. pneumoniae.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00406659
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    Isolation of cyanobacteria from the aquatic fern, Azolla (1979)
    Springer
    Archives of microbiology 120 (1979), S. 161-165 
    Details
    ISSN: 1432-072X
    Keywords: Azolla ; Cyanobacteria ; Blue-green algae ; Nitrogen fixation ; Symbiont ; Photoheterotroph ; Algal isolation ; Photosynthesis ; Plant metabolism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A procedure has been developed to isolate cyanobacteria from the aquatic fern Azolla. The method is based upon recovery of cyanobacterial “bundles” from digests of plants and use of this material as a massive inoculum for nitrogen-free media, followed by prolonged incubation in light. The procedure appears to select for those cells capable of growth in vitro. Isolated cyanobacteria were found to resemble Anabaena sp. morphologically but were capable of heterotrophic growth and had high nitrogenase activity when grown on fructose in the dark.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00409103
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 5
    Electronic Resource
    Electronic Resource
    Hydrogen formation by cyanobacteria cultures selected for nitrogen fixation (1979)
    Springer
    Archives of microbiology 123 (1979), S. 227-232 
    Details
    ISSN: 1432-072X
    Keywords: Hydrogen production ; Nitrogen fixation ; Photosynthesis ; Cyanobacteria ; Enrichment cultures
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Seventy-one cyanobacteria containing cultures were enriched from various soil and water locations either under aerobic and/or anaerobic conditions on agar medium selective for nitrogen fixation. Kept under argon containing 1% CO2 for 24 and 48 h most of these cultures evolved hydrogen at very variable rates up to 116 μl per mg chlorophyll and hour as a mean value over a time period of 24h. Several samples evolved hydrogen more efficiently compared with known hydrogen producing pure strains from culture collections. Thirty-one of the investigated cultures showed a hydrogen formation higher than 10 μl per mg chlorophyll and hour measured over 24 or 48 h. Among these all the morphological forms of cyanobacteria i.e. unicellular and filamentous with or without heterocysts are found. Hence, selecting for nitrogen fixing cyanobacteria seems to be a practical method to find efficient hydrogen producers.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00406654
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 6
    Electronic Resource
    Electronic Resource
    Modes of electron transfer from molecular hydrogen in Anabaena cylindrica (1979)
    Springer
    Archives of microbiology 123 (1979), S. 37-45 
    Details
    ISSN: 1432-072X
    Keywords: Cyanobacteria ; Blue-green algae ; Hydrogen metabolism ; Nitrogen fixation ; Isolated heterocysts ; Inhibitors ; Plastoquinone
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Several natural and artificial electron donors were assayed in the C2H2-reduction of heterocysts isolated from the cyanobacterium Anabaena cylindrica. Among these, molecular hydrogen was the most effective one when the assays were performed in the light. The C2H2-reduction and the Knallgas reaction of intact Anabaena filaments as well as the H2-supported C2H2-reduction of isolated heterocysts were compared for their sensitivity towards several inhibitors known to affect the photosynthetic or respiratory electron flow. Among these, dibromothymoquinone (DBMIB) affected all three reactions equally indicating that plastoquinone is a common intermediate of the H2-consumptions by either the respiratory or the photosynthetic electron transport. Metronidazole inhibited the H2-utilization via photosynthesis but did not affect the consumption of this gas by respiration and therefore allows to differentiate between the two pathways of hydrogen utilization. The studies with the inhibitors are suggestive for a segment of electron carriers on the membranes common to both photosynthesis and respiration in heterocysts of Anabaena.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00403500
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 7
    Electronic Resource
    Electronic Resource
    Isolation and characterization of rapidly-growing, marine, nitrogen-fixing strains of blue-green algae (1979)
    Springer
    Archives of microbiology 121 (1979), S. 155-159 
    Details
    ISSN: 1432-072X
    Keywords: Blue-green algae ; Nitrogen fixation ; Anabaena ; Cyanobacteria ; Marine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Five strains of heterocystous blue-green algae capable of high rates of growth and nitrogenase activity were isolated from shallow coastal environments. Growth of the organisms was characterized with respect to temperature, NaCl concentration in the medium, and nitrogen source. The temperature optima ranged from 35–42°C, and all but one of the strains displayed a requirement for added NaCl. The generation times under N2-fixing conditions were 5.1–5.9 h, and were as low as 3.4 h for growth on NH4Cl. Nitrogenase activity (C2H2 reduction) was high throughout the logarithmic growth phase of each strain. The maximum value observed for one strain was 65.5 nmoles C2H4 produced/mg protein x min, and the average values for the five strains ranged from 24.5–46.7 nmoles C2H4/mg protein x min. The organisms all belong to the genusAnabaena. The growth and nitrogenase activity of these strains are much higher than those of the heterocystous blue-green algae commonly used for investigation of nitrogen metabolism, and they thus should prove to be useful physiological tools. Their prevalence, as judged by the ease of their enrichment and isolation, in bay and estuarine environments suggests that they are important contributors of combined nitrogen.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00689980
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 8
    Electronic Resource
    Electronic Resource
    The biology ofAzospirillum-sugarcane association II. Ultrastructure (1979)
    Springer
    Protoplasma 101 (1979), S. 143-163 
    Details
    ISSN: 1615-6102
    Keywords: Azospirillum ; Nitrogen fixation ; Sugarcane ; Tissue culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Tissue cultures of sugarcane support abundant growth ofAzospirillum brasilense (SP 7). Visible after 1–2 weeks as a white or pink slime, this growth reaches 2×108 bacteria/mm2 on the surface of callus. Growth of the bacterium is strictly extracellular in viable callus, and instances of intracellular growth result from rupture of the cell wall during senescence of callus tissue. A significant proportion of the bacterial population on callus is pleomorphic. Varying the nitrogen source in the nutrient medium caused no obvious effect on callus cell structure. The presence of the bacterium caused structural alterations in callus cells which did not inhibit overall growth of the bacterium. Growth of callus as tight groups of cells lacking intercellular spaces may be important for the establishment of a long-term association withAzospirillum. The interface of bacteria and live callus tissue is at the surface of tight cell groups. Browning of the surface cell layers of these groups in the presence ofAzospirillum is not of the rapid nature known for hypersensitivity reactions. Rather, this production of phenolics appears to be due to the accumulation of extracellular bacterial metabolites. The ultrastructure of this and other callus reactions is described. As evidenced by organogenesis, the associated cultures have remained viable for at least 18–20 months.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01281569
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 9
    Electronic Resource
    Electronic Resource
    Nitrogen-fixing bacillus species from Egyptian soils: Acetylene reduction and cultural conditions (1979)
    Springer
    Plant and soil 51 (1979), S. 187-196 
    Details
    ISSN: 1573-5036
    Keywords: Acetylene reduction ; Aerobic ; Anaerobic ; Bacillus ; Nitrogen fixation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Among 390 isolates from Egytiian soils initially grown on Brown's N-free agar, 15 facultative Bacillus isolates were able to reduce acetylene in Stanier's N-poor broth under both aerobic and anaerobic (N2 atmosphere) conditions. Some of these isolates were Gram-positive, with unswollen sporangia and thin-walled endospores. Other strains were with slightly or definitely bulged sporangia. Yeast extract (0.01%) was essential for growth stimulation and N2[C2H2] fixation by these isolates. Replacing yeast extract with 20 μg/ml (NH4)2SO4 or biotin, thiamine and amino acids (singly or in combination) resulted in stimulation of growth and N2[C2H2] fixation, though at lower rates than in yeast extract. One isolate was able to grow and reduce C2H2 in Stanier's N-free liquid medium. Nitrogenase [C2H2] activity of the anaerobically grown and incubated cultures was greater than aerobic cultures. Addition of 0.1% CaCO3 to the culture media significantly increased and O2 partially inhibited, N2[C2H2] fixation by these Bacillus isolates. Studies of the characteristics and N2[C2H2] fixing activities of these isolates indicate that at least some of them are new nitrogen-fixingBacillus species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02232882
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 10
    Electronic Resource
    Electronic Resource
    Influence of a carbamate pesticide on growth, respiration (14C)-carbon metabolism and symbiosis of aRhizobium sp. (1979)
    Springer
    Plant and soil 51 (1979), S. 355-361 
    Details
    ISSN: 1573-5036
    Keywords: Aldicarb ; 14C-carbon metabolism ; Cowpea ; Growth ; Nitrogen fixation ; Nodulation ; Pesticide ; Respiration ; Rhizobium ; Soil ; Symbiosis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Addition of aldicarb (2 methyl-2(methyl thio) propionaldehyde-0-methyl carbamoyl oxime) in the growth medium enhanced the growth ofRhizobium sp. (cowpea group) at 2 ppm level while an inhibition was observed at the normal (5 ppm) and higher (10 ppm) concentrations. Respiration of the cells was also inhibited by 5 and 10 ppm levels of the chemical eventhough a stimulation was observed at 2 ppm (lower) concentration. The insecticide, when incorporated at 5 and 10 ppm levels in the medium increased the14C-glucose incorporation and considerably altered the assimilation of the radioactive carbon in different fractions of rhizobium cells. Soil application of this insecticide (Temik 10 G) reduced the number of nodules formed and the total nitrogen content in cowpea plants inoculated with theRhizobium sp. but enhanced the dry matter production of cowpea plants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02197782
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...