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  • Zea  (23)
  • Spektralphotometrie, Atomabsorption  (19)
  • Immunocytochemistry  (17)
  • Springer  (59)
  • 1975-1979  (59)
  • 1978  (59)
Collection
Keywords
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  • Springer  (59)
Years
  • 1975-1979  (59)
Year
  • 1
    Electronic Resource
    Electronic Resource
    A comparison of regenerated cell walls in tobacco and cereal protoplasts (1978)
    Springer
    Planta 139 (1978), S. 155-158 
    Details
    ISSN: 1432-2048
    Keywords: Avena ; Cell-wall regeneration ; Electrical potential ; Nicotiana ; Protoplast culture ; Turgor pressure ; Zea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Four independent kinds of observations indicate that the cell wall regenerated by oat (Avena sativa L.) and corn (Zea mays L.) protoplasts in culture is less well developed than that regenerated by tobacco (Nicotiana tabacum L.) protoplasts. Following wall regeneration the cereal protoplasts remained susceptible to osmotic shock upon transfer to water, showed great enlargement, stained poorly with calcofluor white, and maintained a positive internal electrical potential. The development of a negative membrane potential by tobacco protoplasts in culture often occurred simultaneously with the onset of cell division. Since division was observed only in protoplasts which had regenerated good cell walls and had re-established negative membrane potentials it is suggested that culture conditions which favor these two processes should improve protoplast viability.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00387141
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  • 2
    Electronic Resource
    Electronic Resource
    Properties of auxin binding sites in different subcellular fractions from maize coleoptiles (1978)
    Springer
    Planta 140 (1978), S. 97-106 
    Details
    ISSN: 1432-2048
    Keywords: Auxin binding ; Coleoptiles ; Multiple receptors ; Tonoplast ; Zea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In-vitro binding of labeled auxins to sedimentable particles was tested in subcellular fractions from homogenates of maize (Zea mays L.) coleoptiles. The material was fractionated by differential centrifugation or on sucrose density gradients. It was confirmed that the major saturable binding activity (site I) for 1-naphthyl[1-14C]acetic acid is associated with vesicles derived from the endoplasmatic reticulum. A second type of specific auxin binding (site II) could be distinguished by several criteria, e.g. by the low affinity towards phenylacetic acid. The particles carrying site II could be clearly separated from markers of the endoplasmatic reticulum, the plasmalemma, the mitochondria and the nuclei, while their density as well as sedimentation velocity correlated with particle-bound acid phosphatase, indicating a localization at the tonoplast. In contrast to site I, binding at site II was hardly affected by a supernatant factor and by sulfhydryl groups. However, the specificity pattern of site II towards auxins and auxin analogs was very similar to that of site I tested in the presence of supernatant factor. The existence of a third auxin receptor localized in plasma membrane-rich gradient fractions was indicated by a preferential in-vitro binding of 2,4-dichlorophenoxyacetic acid.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00384907
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  • 3
    Electronic Resource
    Electronic Resource
    Continuous measurement of initial curvature of maize coleoptiles induced by lateral auxin application (1978)
    Springer
    Planta 140 (1978), S. 201-211 
    Details
    ISSN: 1432-2048
    Keywords: Auxin ; Cell elongation ; Coleoptiles ; Fusicoccin ; Zea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract To analyze early effects of auxin application, an apparatus was developed which continuously and simultaneously registered the curvature of 10 individual maize (Zea mays L.) coleoptiles. Resolution was less than 5 μm over a range of ±0.5 mm. The data were evaluated and plotted via paper tape and Hewlett-Packard-computer. Unilateral application of 3×10-5 M indoleacetic acid (IAA) resulted in a transient inhibition of growth on the side of application for ca. 10 min (Phase I), followed by a strong stimulation (Phase II). The phytotoxin fusicoccin (FC) caused an immediate stimulation of elongation. The initial negative reaction of Phase I is auxin-specific. Only active auxins such as IAA and 1-naphtaleneacetic acid produced this initial inhibition; chemical analogs-inhibitory or neutral in long-term growth tests, e.g. phenylacetic acid-did not show any significant effects on Phase I. When the coleoptiles were symmetrically preloaded with different levels of auxin, only a large step-up of subsequent unilateral auxin application resulted in a negative phase I; a small step-up led to an immediate positive reaction. The results are discussed in context with the parallel kinetics for various other auxin-induced reactions of coleoptile cells which have already been published.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00390249
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  • 4
    Electronic Resource
    Electronic Resource
    Light-induced fluorescence decay during the greening of normal and lincomycin-treated maize leaves (1978)
    Springer
    Planta 141 (1978), S. 135-139 
    Details
    ISSN: 1432-2048
    Keywords: Chlorophyll (fluorescence) ; Fluorescence (chlorophyll) ; Lincomycin ; Zea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Light-induced fluorescence decay was examined during the greening of control and lincomycintreated maize (Zea mays L.) leaves. Assuming that this decay to a first approximation is the result of two parallel first-order reactions, the fluorescence induction curves were linearized on the logarithm plot and the parameters were determined. The variable fluorescence increased, and the parameters of the two linear sections of the fluorescence decay—that is, the kinetics of the induction curves—changed during the greening of the control leaves. Lincomycin treatment caused some chlorophyll deficiency and the lowering of the chlorophyll a/b ratio, changed the fluorescence emission spectra and the effect of Mg2+ on the regulation of the excitation energy distribution. The structure of the thylakoids and the kinetics of the fluorescence decay were also changed in the treated leaves. The possible relationship between the change of the kinetics of the fluorescence decay and the change of spillover during greening and after lincomycin treatment is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00387879
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  • 5
    Electronic Resource
    Electronic Resource
    Changes in the secretory activity of the Golgi apparatus during the cell cycle in root tips of maize (Zea Mays L.) (1978)
    Springer
    Planta 138 (1978), S. 113-118 
    Details
    ISSN: 1432-2048
    Keywords: Cell division ; Cell wall ; Golgi apparatus ; Secretory vesicles ; Zea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Epidermal cells of maize roots were studied to determine the distribution of Golgi apparatus-derived secretory vesicles in various stages of cell division. The following conclusions were reached: 1) The pattern of Golgi apparatus secretion varies with the cell cycle. 2) Large numbers of secretory vesicles are incorporated into the cell plate. 3) Secretory vesicles from the Golgi apparatus are incorporated primarily in walls undergoing expansion. 4) Secretory vesicles are smaller during mitosis and the first part of cytokinesis than they are during interphase. 5) Secretory vesicles account for at least 12–23% of cell-plate plasma membrane and an estimated 25% of cell-plate volume.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00391165
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  • 6
    Electronic Resource
    Electronic Resource
    α-and β-Glycosidases in maize roots (1978)
    Springer
    Planta 138 (1978), S. 119-122 
    Details
    ISSN: 1432-2048
    Keywords: Glycosidases ; Root ; Zea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Four glycosidases were analyzed in 10 mm apical segments prepared from growing roots (15 mm) of Zea mays L. The pH optima were found to be 5.8 for β-glucosidase, 4.4 for β-galactosidase, 6.4 for α-glucosidase and 6.0 for α-galactosidase. The β-glucosidase showed 4-fold higher activity than the β-galactosidase. The distribution of the β-glucosidase activity was signifcantly different from that of the β-galactosidase, α-glucosidase and α-galactosidase.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00391166
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  • 7
    Electronic Resource
    Electronic Resource
    3-O-Methyl glucose uptake stimulation by auxin and by fusicoccin in plant materials and its relationships with proton extrusion (1978)
    Springer
    Planta 138 (1978), S. 249-256 
    Details
    ISSN: 1432-2048
    Keywords: Auxin ; Fusicoccin ; Glucose transport ; Proton flux ; Roots ; Zea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Auxin and fusicoccin (FC) stimulate the active uptake of 3-O-methyl glucose (3-O-MG) in those materials in which they have been shown to activate an electrogenic proton extrusion (Pisum sativum L. stems, Zea mays L. coleoptiles and roots). In maize roots the curve relating 3-O-MG influx to external concentrations indicated that the values of the apparent Km increase in the 3-O-MG concentration range between 2×10-5 mol l-1 and 2×10-2 mol l-1. FC did not alter the Km values and its stimulating effect was nearly constant at all 3-O-MG concentrations tested. Basal and FC-induced uptake of 3-O-MG appeared associated with a transient proton influx suggesting that also in maize roots a sugar-proton contransport occurs. Diethyl stilbestrol, which inhibits proton extrusion, inhibited also basal and FC-induced 3-O-MG uptake. The data support the view that the stimulation by FC of 3-O-MG uptake is closely related to that of proton extrusion. The stimulation by FC of 3-O-MG uptake cannot be replaced by increasing extracellular proton concentration, nor may be explained only by the FC-induced hyperpolarization of transmembrane potential difference. The hypothesis is proposed that the effect of FC on 3-O-MG uptake depends on an increase of cytoplasmic pH, following the activation of the proton extruding system.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00386819
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  • 8
    Electronic Resource
    Electronic Resource
    Phytochrome photoreversibility: Empirical test of the hypothesis that it varies as a consequence of pigment compartmentation (1978)
    Springer
    Planta 141 (1978), S. 129-134 
    Details
    ISSN: 1432-2048
    Keywords: Avena ; Immunocytochemistry ; Phytochrome
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Phytochrome of oat (Avena sativa L., cv. Garry) coleoptile cells in the red-light-absorbing form, Pr, is diffusely distributed while after conversion to the far-red-light-absorbing form, Pfr, it is observed only in very small areas within the cell. Comparison of phytochrome photoversibility measurements to the distribution of the pigment within the cell indicates that the spectral assay is not influenced by the observed compartmentalization of the chromoprotein. However, the observed compartmentalization of phytochrome is correlated with a loss in spectrophotometrically detectable Pr.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00387878
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  • 9
    Electronic Resource
    Electronic Resource
    Auxin gradient along the root of the maize seedling (1978)
    Springer
    Planta 141 (1978), S. 179-181 
    Details
    ISSN: 1432-2048
    Keywords: Auxin ; Maize ; Root tip ; Zea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract [5-3H]Indol-3yl-acetic acid (IAA) applied to the shoot apices of intact 6-day-old maize (Zea mays L.) plants moved into the primary root and accumulated at the root apex. IAA from the shoot could partially satisfy the requirement of the primary root for IAA for growth.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00387886
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  • 10
    Electronic Resource
    Electronic Resource
    Identification of embryonal antigens of maize: Globulins as primary reserve proteins of the embryo (1978)
    Springer
    Planta 143 (1978), S. 11-20 
    Details
    ISSN: 1432-2048
    Keywords: Antigen spectra ; Embryo (proteins) ; γ-Plantlets ; Germination (seeds) ; Globulins ; Proteins (storage) ; Seedling growth ; Storage proteins ; Zea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Normal and γ-irradiated caryopses of Zea mays L. were germinated, and the degradation of embryonal antigens (EA) was followed in the endosperms, scutella and embryonic axes of the seedlings, using double immunodiffusion, immunoelectrophoresis and quantitative immunoprecipitation. The predominant transient EA were presumed to be storage proteins related to the reserve globulins of dicotyledonous seeds. Therefore globulins were isolated from maize scutella, purified by (NH4)2SO4 fractionation and isoelectric precipitation, and the molecular weights of the polypeptide units were estimated by discontinuous sodium-dodecyl-sulphate slab electrophoresis. The globulins were found to be identical with the predominant EA and amounted to about 40% of the protein nitrogen in the embryos of mature, non-germinated caryopses. The presumed reserve function of the globulins and the characteristic time course of their degradation in embryonic axes and scutella of maize seedlings are discussed in relation to the two-step pattern of mobilization of nitrogen reserves in germinating cereal caryopses.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00389046
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