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  • Pisum  (19)
  • Springer  (19)
  • American Chemical Society
  • American Chemical Society (ACS)
  • 1975-1979  (19)
  • 1978  (19)
Collection
Keywords
Publisher
  • Springer  (19)
  • American Chemical Society
  • American Chemical Society (ACS)
Years
  • 1975-1979  (19)
Year
  • 1
    Electronic Resource
    Electronic Resource
    Growth of Avena coleoptiles and pH drop of protoplast suspensions induced by chlorinated indoleacetic acids (1978)
    Springer
    Planta 140 (1978), S. 89-92 
    Details
    ISSN: 1432-2048
    Keywords: Auxin ; Avena ; Helianthus ; pH drop ; Pisum ; Protoplast suspension
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Several indoleacetic acids, substituted in the benzene ring, were compared in the Avena straight growth bioassay. 4-Chloroindoleacetic acid, a naturally occurring plant hormone, is one of the strongest hormones in this bioassay. With an optimum at 10-6 mol l-1, it is more active than indoleacetic acid, 2,4-dichlorphenoxyacetic acid and naphthaleneacetic acid. 5-Chloro- and 6-chloroindoleacetic acids are very strong auxins as well. Other derivatives tested have a lower activity. 5,7-Dichloro- and 5-hydroxyindoleacetic acids have very low auxin activity at 10-4 mol l-1 and may be anti-auxins. Some of the derivatives were compared for their effect on pH decline in stem protoplast suspensions of Helianthus annuus L. and Pisum sativum L. The change of pH occurs without a lag period or with only a very short one. Derivatives which are very active in the Avena straight growth assay cause a larger pH decline than indoleacetic acid, while inactive derivatives cause effectively no pH decline.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00389385
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  • 2
    Electronic Resource
    Electronic Resource
    Concanavalin A-mediated agglutination of plant plastids (1978)
    Springer
    Planta 138 (1978), S. 35-39 
    Details
    ISSN: 1432-2048
    Keywords: Agglutination ; Chloroplasts ; Concanavalin A ; Cucumis ; Pisum ; Pyrus ; Proplastids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Cucumber (Cucumis sativus L.) and pear (Pyrus domestica Medik.) fruit proplastids, and pea (Pisum sativum L., cv. Meteor) leaf chloroplasts, extracted by osmotic rupture of protoplasts isolated after degradation of the cell walls by cellulase and pectinase, agglutinated in the presence of Con A. Agglutination of cucumber proplastids was inhibited by anti-Con A and by methyl α D-gluco/manno pyranosides but not by methyl α D-galactopyranoside. Fluorescein isothiocyanate-conjugated Con A (FITC-Con A) rendered agglutinated clumps fluorescent. If cellulase was omitted from the macerating medium, Con A-mediated agglutination did not occur even if proplatids were subsequently incubated with cellulase. Proplastids and chloroplasts extracted by conventional mechanical disruption methods were not agglutinated by Con A and did not acquire fluorescence with FITC-Con A. However, cucumber proplastids so extracted could be agglutinated by Con A if incubated with cellulase after preparation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00392911
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  • 3
    Electronic Resource
    Electronic Resource
    Correlation between deoxyribonuclease activity and DNA replication in the embryonic axes of germinating peas (Pisum sativum L.) (1978)
    Springer
    Planta 138 (1978), S. 99-103 
    Details
    ISSN: 1432-2048
    Keywords: Chromatin ; DNA ; Germination ; Pisum ; Replication (DNA)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Crude chromatin preparations from pea seedlings contain calcium-dependent deoxyribonuclease activity, at least part of which is endonucleolytic. During germination, there is a dramatic increase in chromatin-bound deoxyribonuclease activity in the embryonic axis immediately prior to the onset of DNA replication. Evidence has been obtained for the presence of an inhibitor of deoxyribonuclease activity in chromatin preparations from embryonic axes not undergoing DNA replication.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00392923
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  • 4
    Electronic Resource
    Electronic Resource
    Asymbiotic association of Rhizobium with pea epicotyls treated with a plant hormone (1978)
    Springer
    Planta 138 (1978), S. 107-110 
    Details
    ISSN: 1432-2048
    Keywords: Auxin ; Nitrogen fixation (asymbiotic) ; Pisum ; Rhizobium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Treatment of epicotyls of dark-grown pea (Pisum sativum L.) seedlings with indole-3-acetic acid causes swelling of the tissue. Application of Rhizobium to the cut surface of the swollen tissue results in the development of an “infection”. The infection spreads in the cortical cells and proceeds 2–3 mm deep into the stem within 3–4 days. An acetylene reduction assay used for detecting nitrogen-fixation capacity of the infected tissue was negative at 10% [O2]; however, if [O2] was reduced to below 1%, some activity could be detected. Ultrastructural observations indicate that the cytoplasmic contents of the infected cells are destroyed and no membrane structure around the bacteria is formed during this infection. Rhizobium does not appear to have developed any symbiotic relationship with the host. Failure to develop symbiosis appears to result in a parasitic or saprophytic association and the nitrogen fixed under such conditions may not be of any use to the plant.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00392925
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  • 5
    Electronic Resource
    Electronic Resource
    Partial purification and characterization of the soluble DNA polymerase (polymerase-α) from seedlings of Pisum sativum L. (1978)
    Springer
    Planta 138 (1978), S. 127-132 
    Details
    ISSN: 1432-2048
    Keywords: DNA polymerase ; Pisum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Soluble DNA polymerase has been extracted from pea seedlings and partially purified by chromatography on columns of DEAE-cellulose or DEAE-Sephadex. The enzyme elutes from DEAE-cellulose as a single peak, but is fractionated into three peaks, SI, SIa and SII by DEAE-Sephadex chromatography. SIa and SII may be derived from SI by freeze-thaw treatment or by treatment with (NH4)2SO4. The ion and pH requirements and the sensitivity to N-ethyl maleimide of the pea seedling soluble DNA polymerase are similar to those of the DNA polymerase-α from vertebrates.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00391168
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  • 6
    Electronic Resource
    Electronic Resource
    The effect of neutral salt anions on the oxidative deamination activity of plant glutamate dehydrogenase (1978)
    Springer
    Planta 138 (1978), S. 161-165 
    Details
    ISSN: 1432-2048
    Keywords: Activity interconversion ; Chaotropic anions ; Cooperative effect ; Enzyme properties ; Glutamate dehydrogenase ; Pisum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Increasing concentrations of anions of the Hofmeister series decrease the activity of highly purified glutamate dehydrogenase (EC 1.4.1.2.) from Pisum sativum L. The extent of the inactivation, as estimated by the ion concentration which causes a 50% transformation of the native form to the low activity form of the enzyme (approximately “halfmaximal activity”), follows the ranking Cl−〈F−〈Br−〈NO 3 − 〈SCN−. Sulfate has a slightly activating effect. At salt concentrations higher than 1 M (with SCN− higher than 200 mM), the activity decreases to a value from 3–6% of the initial activity and remains then stable over a wide range of higher anion concentrations. From kinetic investigations it is seen that the treatment of the enzyme with anions decreases the affinity for the cosubstrate NAD+ and the substrate L-glutamate (K M-values increased) and also increases the dissociation constant for NAD+. The salt induced inactivation is reversible by dilution. From a mathematical treatment of the kinetic data of the inactivation, it is seen that increasing concentrations of the anions exert cooperative effects on the inactivation process.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00391173
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  • 7
    Electronic Resource
    Electronic Resource
    Cytochemical localization of adenosine triphosphatase in the phloem of Pisum sativum and its relation to the function of transfer cells (1978)
    Springer
    Planta 140 (1978), S. 111-120 
    Details
    ISSN: 1432-2048
    Keywords: ATPase ; Phloem ; Pisum ; Transfer cells (phloem) ; Translocation (short-distance)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The cytochemical localization of ATPase in differentiating and mature phloem cells of Pisum sativum L. has been studied using a lead precipitation technique. Phloem transfer cells at early stages of differentiation exhibit strong enzyme activity in the endoplasmic reticulum (ER) and some reaction product is deposited on the vacuolar and plasma membranes. As the phloem transfer cells mature and develop their characteristic wall structures, strong enzyme activity can be observed in association with the plasma membranes and nuclear envelopes. Mature phloem transfer cells with elaborate cell-wall ingrowths show ATPase activity evenly distributed on plasma-membrane surfaces. Differentiating sieve elements show little or no enzyme activity. When sieve elements are fully mature they have reaction product in the parietal and stacked cisternae of the ER. There is no ATPase activity associated with P-protein at any stage of sieve-element differentiation or with the sieve-element plasma membranes. It is suggested that the intensive ATPase activity on the plasma membranes of the transfer cells is evidence for a transport system involved in the active movement of photosynthetic products through these cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00384909
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  • 8
    Electronic Resource
    Electronic Resource
    Photoperiod-induced changes in gibberellin metabolism in relation to apical growth and senescence in genetic lines of peas (Pisum sativum L.) (1978)
    Springer
    Planta 141 (1978), S. 231-238 
    Details
    ISSN: 1432-2048
    Keywords: Apical senescence ; Gibberellins ; Photomorphogenesis ; Pisum ; Senescence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In an early-flowering line of pea (G2) apical senescence occurs only in long days (LD), while growth in short days (SD) is indeterminate. In SD, G2 plants are known to produce a graft-transmissible substance which delays apical senescence in related lines that are photoperiod-insensitive with regard to apical senescence. Gibberellic acid (GA3) applied to the apical bud of G2 plants in LD delayed apical senescence indefinitely, while N6-benzyladenine and α-naphthaleneacetic acid were ineffective. Of the gibberellins native to pea, GA9 had no effect whereas GA20 had a moderate senescence-delaying effect. [3H]GA9 metabolism in intact leaves of G2 plants was inhibited by LD and was restored by placing the plants back in SD. Leaves of photoperiod-insensitive lines (I-types) metabolized GA9 readily regardless of photoperiod, but the metabolites differed qualitatively from those in G2 leaves. A polar GA9 metabolite, GAE, was found only in G2 plants in SD. The level of GA-like substances in methanol extracts from G2 plants dropped about 10-fold after the plants were moved from SD to LD; it was restored by transferring the plants back to SD. A polar zone of these GA-like materials co-chromatographed with GAE. It is suggested that a polar gibberellin is synthesized by G2 plants in SD; this gibberellin promotes shoot growth and meristematic activity in the shoot apex, preventing senescence.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00388337
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  • 9
    Electronic Resource
    Electronic Resource
    Metabolism of gibberellin A29 in seeds of Pisum sativum cv. Progress No. 9; Use of [2H] and [3H]GAs, and the identification of a new GA catabolite (1978)
    Springer
    Planta 144 (1978), S. 69-78 
    Details
    ISSN: 1432-2048
    Keywords: Gas chromatography-mass spectrometry ; Gibberellin metabolism ; Internal standards ; Isotope ratios ; Pisum ; Seeds (gibberellins)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The metabolism of GA29 in maturing seeds of Pisum sativum cv. Progress No. 9 was further investigated, and the utility of 2H-labelled GAs in conjuction with GC-MS is illustrated. Using [2α-2H1]GA29 as an internal standard, endogenous GA29 was shown to reach a maximal level (ca. 10 μg/seed) 27 days from anthesis, and to decline to ca. 1.6 μg/seed in mature seeds. In a time-course feed the metabolism of [2α-2H1] [2α-3H1]GA29 applied to 27 day old seeds, and of endogenous GA29, was compared from the 1H:2H ratios in the recovered GA29. Although both [2α-2H1] [2α-3H1]GA29 and endogenous GA29 were metabolised to the same limited extent to a putative conjugate, in the main metabolic process endogenous GA29 was preferentially converted to an untraceable (i.e. unlabelled) metabolite. In contrast, endogenous GA29 and [1β,3α-2H2] [1β,3α-3H2]GA29, derived from [1β,3α-2H2] [1β,3α-3H2]GA20 in a time-course feed, were metabolised in an identical manner. In the latter case isotope loss precluded identification of the metabolite. The structure (8) has been assigned to a GA catabolite present in maturing seeds and seedlings of pea. The isotope data are consistent with this compound being the hitherto untraced metabolite of GA29 in pea.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00385009
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  • 10
    Electronic Resource
    Electronic Resource
    Interaction of gibberellic and indole-3-acetic acid on root formation in pea (Pisum sativum L.) epicotyl cuttings (1978)
    Springer
    Planta 143 (1978), S. 331-332 
    Details
    ISSN: 1432-2048
    Keywords: auxin ; Gibberellin ; Interaction (hormones) ; Pisum ; Root formation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Indole-3-acetic acid (IAA) strongly enhanced rooting of etiolated pea epicotyl cuttings while gibberellic acid (GA3) enhanced rooting only slightly. The promoting effects of the hormones appeared not until 14 d after the onset of treatment. When GA3 and IAA were applied together, the initiation of rooting started already after 6 d after onset of treatment. It is suggested that gibberellin plays an important role, in combination with auxin, in the initiation of root formation in Pisum cuttings.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00392007
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