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  • Spektralphotometrie, Atomabsorption  (19)
  • Immunocytochemistry  (17)
  • Springer  (36)
  • 1995-1999
  • 1980-1984
  • 1975-1979  (36)
  • 1925-1929
  • 1978  (36)
Collection
Keywords
Publisher
  • Springer  (36)
Years
  • 1995-1999
  • 1980-1984
  • 1975-1979  (36)
  • 1925-1929
Year
  • 1
    Electronic Resource
    Electronic Resource
    Phytochrome photoreversibility: Empirical test of the hypothesis that it varies as a consequence of pigment compartmentation (1978)
    Springer
    Planta 141 (1978), S. 129-134 
    Details
    ISSN: 1432-2048
    Keywords: Avena ; Immunocytochemistry ; Phytochrome
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Phytochrome of oat (Avena sativa L., cv. Garry) coleoptile cells in the red-light-absorbing form, Pr, is diffusely distributed while after conversion to the far-red-light-absorbing form, Pfr, it is observed only in very small areas within the cell. Comparison of phytochrome photoversibility measurements to the distribution of the pigment within the cell indicates that the spectral assay is not influenced by the observed compartmentalization of the chromoprotein. However, the observed compartmentalization of phytochrome is correlated with a loss in spectrophotometrically detectable Pr.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00387878
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  • 2
    Electronic Resource
    Electronic Resource
    Anreicherung und Bestimmung von Blei in Boden- und Sedimentaufschlüssen und -extrakten mit der Flammenatomabsorption (1978)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 291 (1978), S. 213-216 
    Details
    ISSN: 1618-2650
    Keywords: Best. von Blei in Boden, Sedimenten ; Spektralphotometrie, Atomabsorption ; Flamme, Reextraktion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Zusammenfassung In Vorversuchen wurde gezeigt, da\ beim Aufbewahren der organischen APDC/MIBK-Extrakte starke Bleiverluste auftraten. Daher war es notwendig, das Blei mit SalpetersÄure in die wÄ\erige Phase zu reextrahieren. Wiederfindungsversuche bestÄtigten, da\ Extraktion und Reextraktion quantitativ verlaufen. Das Blei wird dabei um den Faktor fünf angereichert. Es werden mittlere Standardabweichungen für vier Konzentrationsbereiche angegeben. Als Nachweisgrenze wurde eine Konzentration von 0,16 mg Pb/l gefunden, die durch die Anreicherung auf ca. 0,03 mg Pb/l gesenkt wird. Arbeitsvorschriften für das Verfahren sind angegeben.
    Notes: Summary Preliminary investigations have shown, that considerable losses of lead occur during storage of the organic APDC/MIBK-extracts. Therefore, it was necessary to reextract lead into the aqueous phase by nitric acid. Investigations of recoveries confirmed, that extraction and reextraction of lead were quantitative. In this way the concentration of lead is increased by a factor of five. Mean standard deviations for four concentration ranges are given. A detection limit of 0.16 mg of Pb/l is obtained, which is lowered by the preceding enrichment to about 0.03 mg of Pb/l. Details of the procedure are given.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00480692
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  • 3
    Electronic Resource
    Electronic Resource
    Zur AAS-Bestimmung von Quecksilber in Zahnwurzeln und Kieferknochen (1978)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 293 (1978), S. 141-144 
    Details
    ISSN: 1618-2650
    Keywords: Best. von Quecksilber in Zähnen, Knochen ; Spektralphotometrie, Atomabsorption ; HNO3/HF-Aufschluß, Kaltdampfverfahren
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Zusammenfassung Es wird ein Verfahren zur Bestimmung von Quecksilber in Zahnwurzeln und Kieferknochen beschrieben. Die homogenisierten Proben werden in Teflonbomben unter Druck mit einem Gemisch von HNO3/HF aufgeschlossen. Die Quecksilberbestimmung erfolgt mit einem Atomabsorptionsspektrophotometer nach dem Kaltdampfverfahren. Unter Einsatz von 200 mg Probe können Quecksilbergehalte bis zu 0,1 μg/g bestimmt werden. Die relativen Standardabweichungen liegen im Arbeitsbereich von 10–100 ng Quecksilber zwischen 2,7 und 5,6 %. Es wurden Quecksilbergehalte von 〈0,1–187 μg/g in den Proben gefunden.
    Notes: Summary The homogenized samples are decomposed with a mixture of HNO3/HF in teflon vessels under pressure. The determination of mercury is carried out with an atomic absorption spectrophotometer by means of the cold-vapour technique. The limit of detection is 0.1 μg/g for a 200 mg sample. A relative standard deviation of 2.7–5.6% is achieved in the working range of 10–100 ng mercury. Mercury contents of 〈0.1–187 μg/g were found in the samples.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00486994
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  • 4
    Electronic Resource
    Electronic Resource
    Extraction and atomic-absorption spectrometric determination of traces of bismuth with zinc dibenzyldithiocarbamate in aluminium alloys and solder alloys (1978)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 292 (1978), S. 282-284 
    Details
    ISSN: 1618-2650
    Keywords: Best. von Wismut in Aluminiumlegierungen, Lötlegierungen ; Spektralphotometrie, Atomabsorption ; Zinkdibenzyldithiocarbamat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Summary A sensitive method for the extraction and atomic-absorption spectrometric determination of bismuth with zinc dibenzyldithiocarbamate (ZnDBC) has been developed for aluminium alloys and solder alloys. Bismuth is extracted from relatively strong acidic solution into methyl isobutyl ketone (MIBK) as BiDBC complex and its concentration is determined by spraying the MIBK extract into an air-acetylene flame. Iron and copper up to 1 mg resp. 100 μg did not interfere with the extraction of bismuth in the range of 0–80 μg. The limit of detection was about 0.12 ppm in MIBK.
    Notes: Zusammenfassung Ein empfindliches Verfahren zur Wismutbestimmung mit Zinkdibenzyldithiocarbamat wurde ausgearbeitet. Wismut wird dabei aus relativ stark saurer Lösung als Bi-DBC-Komplex mit Methylisobutylketon extrahiert und zur Messung der Extrakt direkt in die Luft-Acetylen-Flamme gesprüht. Bis zu 1 mg Fe und 100 μg Cu stören die Bestimmung von 0–80 μg Bi nicht. Die Nachweisgrenze beträgt 0,12 ppm in Methylisobutylketon.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00493696
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  • 5
    Electronic Resource
    Electronic Resource
    Comparative study of sodium borohydride tablet and tin(II) chloride reducing systems in the determination of mercury by atomic-absorption spectrophotometry (1978)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 292 (1978), S. 273-277 
    Details
    ISSN: 1618-2650
    Keywords: Best. von Quecksilber ; Spektralphotometrie, Atomabsorption ; Kaltdampf, Vergleich der Reduktion mit NaBH4-Tablette oder SnCl2-Lösung
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Summary An atomic-absorption spectrophotometric method for the rapid and precise determination of mercury has been studied by using the sodium borohydride tablet reduction and cold-vapour atomicabsorption procedure coupled with amalgamation on gold. The comparison of the sodium borohydride tablet and tin(II) chloride solution reducing systems are described in detail. The use of the sodium borohydride tablet wrapped in a wafer sheet is very simple and gives more precise results. From the comparison of the effect of diverse ions, it is obvious that both reducing systems have their merits and demerits.
    Notes: Zusammenfassung Ein atomabsorptions-spektralphotometrisches Kaltdampf-Verfahren zur schnellen Quecksilberbestimmung wird beschrieben, das auf der Reduktion mit Hilfe der Natriumborhydridtablette und der Amalgamierung mit Gold beruht. Der Vergleich mit der Zinn(II)-Chloridmethode wird diskutiert. Genaue Ergebnisse werden durch einfaches Einhüllen der Reduktionstablette in ein Waffelblatt erzielt, wodurch der Reaktionsbeginn verzögert wird. In bezug auf Störelemente ergeben sich für beide Verfahren Vor- und Nachteile.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00493694
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  • 6
    Electronic Resource
    Electronic Resource
    Bestimmung von Blei in kleinen Proben der marinen Diatomee Skeletonema costatum (Grev.) Cleve durch flammenlose Atom-Absorptions-Spektrometrie nach Flußsäureveraschung (1978)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 292 (1978), S. 278-281 
    Details
    ISSN: 1618-2650
    Keywords: Best. von Blei in Algen, Diatomeen ; Spektralphotometrie, Atomabsorption ; flammenlos, Störungen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Zusammenfassung Es wird eine nasse HF-HClO4Veraschungsmethode von biologischem Material in offenen PTFE-Reagensgläsern beschrieben. In anschließenden flammenlosen AAS-Messungen mit Hilfe der Deuterium-Untergrundkompensation und des automatischen Probenwechslers AS-1 konnte gezeigt werden, daß Silicium vollständig entfernt worden, kein meßbarer Matrix-Effekt festzustellen war und die Bleikonzentrationsbestimmung durch eine Eichgerade und durch Standard-Addition zu gleichen Werten führte. Die Methode ist mit einem Variations-Koeffizient von 0,45% behaftet.
    Notes: Summary A wet HF-HClO4 decomposition method for biological material in open PTFE-test-tubes is described. Flameless AAS measurements with the aid of deuterium background compensation using the auto sampling system AS-1 showed that Si was completely eliminated by the decomposition, that no measurable matrix effect could be detected, and that the values found for the lead concentration by calibration curve and standard addition were identical. The coefficient of variation for the method was V=0.45%.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00493695
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  • 7
    Electronic Resource
    Electronic Resource
    Determination of organosilicon compounds in water by atomic absorption spectroscopy (1978)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 292 (1978), S. 362-364 
    Details
    ISSN: 1618-2650
    Keywords: Best. von Siliciumorganoverbindungen in Wasser ; Spektralphotometrie, Atomabsorption ; Extraktionsverfahren
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Summary An extraction technique is described for the determination of ppm concentrations of both hydrophobic and hydrophilic methylsiloxane compounds in water, based on a single extraction with a mixed solvent of (1∶1) l-pentanol and methyl isobutyl ketone followed by the determination of the silicone compounds extracted into the mixed solvent by atomic absorption spectroscopy. The method is applicable over the concentration range 0.3–30 ppm Si. Standard deviation in the case of 10 ppm Si is 0.313.
    Notes: Zusammenfassung Zur Bestimmung von ppm-Konzentrationen an hydrophoben und hydrophilen Methylsiloxanverbindungen im Wasser wird ein Extraktionsverfahren beschrieben. Hierbei wird nach einfacher Extraktion mit l-Pentanol/Methylisobutylketon (1∶1) das Silicium im Extrakt mit Hilfe der AAS bestimmt. Der erfaßbare Konzentrationsbereich liegt bei 0,3–30 ppm Si. Die Standardabweichung beträgt bei 10 ppm Si 0,313.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00481552
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  • 8
    Electronic Resource
    Electronic Resource
    Localization of thyrotropin (TSH) in the dog pituitary gland (1978)
    Springer
    Cell & tissue research 186 (1978), S. 399-412 
    Details
    ISSN: 1432-0878
    Keywords: Pituitary gland ; Dog ; Pars distalis ; Thyrotropin (TSH) ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Using the immunoperoxidase technique and antisera to the specific beta (β) subunits of bovine and rat TSH1, selective immunocytochemical staining was localized in a specific cell population in the pars distalis of the dog pituitary gland. These TSH cells were found to be positive to aldehyde fuchsin, alcian blue, periodic acid-Schiff (PAS) and aniline blue. With the performic acidalcian blue (pH 0.2) -PAS-orange G procedure these cells stained blue-purple, demonstrating FSH/LH cells (blue or turquoise), ACTH/MSH cells (redpurple) and PRL cells (orange-red). The TSH cells were further differentiated from other functional cell types of the pars distalis on the basis of their typical cytological features, intraglandular distribution and by immunocytochemical double staining. In the pars distalis of adult male dogs the TSH cells were mostly shown to be smaller in size and less numerous than in bitches in the anestrous phase of the sexual cycle. Moreover, cytological alterations in the immunoreactive thyrotrophs in the pituitary of male and female dogs generally paralleled the spontaneous changes in thyroid function associated with thyroid atrophy and/or pituitary insufficiency, and thyroid hyperplasia or goiter. In conclusion, because of their specificity and high potency, the antisera to the β-subunits of bovine and rat TSH represent an effective tool for the selective immunocytochemical localization of TSH in the dog pituitary. This allows the study of the morphology and function of TSH cells under different physiological, pathological and experimental conditions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00224930
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  • 9
    Electronic Resource
    Electronic Resource
    Immunocytochemical identification of an LHRH-producing system originating in the preoptic nucleus of the duck (1978)
    Springer
    Cell & tissue research 188 (1978), S. 99-106 
    Details
    ISSN: 1432-0878
    Keywords: LHRH-neurosecretion ; Avian hypothalamus ; Vasotocin neurosecretion ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A fluorescent technique applying specific LHRH and vasotocin antisera was used for the immunocytochemical localization of the respective neurosecretory systems in the hypothalamus of gonadectomized, testosteronetreated and/or serotonin injected male domestic ducks. An immunoreactive (IR) LHRH-producing system, with perikarya located in the preoptic nucleus, could be traced through the ventral hypothalamus down to the external layer of the rostral and caudal ME, in close vicinity to the hypophysial portal system. An IR-vasotocin system originating in the paraventricular and supraoptic nuclei ran through the ventral hypothalamus, but terminated in (i) the external layer of the rostral ME, and (ii) in the posterior lobe of the hypophysis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00220517
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  • 10
    Electronic Resource
    Electronic Resource
    Immunocytochemical study of the hypothalamo-neurohypophysial system (1978)
    Springer
    Cell & tissue research 188 (1978), S. 119-132 
    Details
    ISSN: 1432-0878
    Keywords: Neurophysin ; Paraventricular nucleus ; Supraoptic nucleus ; Sheep ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary An antiserum cross-reactive against ovine neurophysins-I-II and -III has been used in conjunction with the immunoperoxidase histochemical procedure to localize the cells of the sheep paraventricular (PVN) and supraoptic nuclei (SON). In order to describe the topographical distribution of the SON and PVN a study was made on the serial sections cut (a) transversely from rostral to caudal positions and (b) sagittally from lateral to medial positions of the hypothalamus. The cells of the SON, when examined in the transverse aspect, extended approximately 1900 μ caudally and when examined in the sagittal plane were contained within a lateral-medial distance of 4830 μ. In each case the SON cells lay adjacent to the optic chiasm. As sections were cut transversely, the cells of the PVN first appeared in a rostral position defined as 0 μ and close to the ventral lining of the third ventricle. This general ventral and ventro-lateral distribution of cells maintained up to a caudal distance of approximately 840 μ. From positions 1260–2310 μ there was a dramatic dorsal shift of the PVN cells which by this time had also extended laterally. The total rostral-caudal distance occupied by the PVN cells was 3150 μ. That the lateral-medial distance occupied by the PVN was small (1050 μ) was determined on examining the magnocellular nuclei in sagittal section.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00220519
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  • 11
    Electronic Resource
    Electronic Resource
    Immunocytological evidence for oxytocin neurons in the human fetal hypothalamus (1978)
    Springer
    Cell & tissue research 188 (1978), S. 259-264 
    Details
    ISSN: 1432-0878
    Keywords: Hypothalamus ; Human fetus ; Oxytocin ; Neurophysin ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The use of antibodies against oxytocin or neurophysin enabled the detection by immunocytochemistry of oxytocin-neurophysin neurons in the hypothalamus in the human fetus. The perikarya of these neurons are located in the paraventricular and supraoptic nuclei. Immunoreactive neurons occur in the median eminence. The neurophysin immunoreactive neurons were more numerous than the oxytocin immunoreactive neurons. The specificity of the immunocytological reaction was controlled. The first oxytocin-neurophysin neurons are seen as early as the 14th week of gestation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00222635
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  • 12
    Electronic Resource
    Electronic Resource
    Immunocytochemical demonstration of calcitonin-containing c-cells in the thyroid glands of different mammals (1978)
    Springer
    Cell & tissue research 186 (1978), S. 551-558 
    Details
    ISSN: 1432-0878
    Keywords: C-cells ; Thyroid gland ; Immunocytochemistry ; Calcitonin antibody ; Mammals
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In the thyroid glands of the horse, pig, deer, mole, and rat, C-cells could be demonstrated by means of the immunocytochemical PAP-technique using rabbit antisera against human calcitonin. Only in ruminants, the crossreaction between the intracellularly stored antigen and the antibodies used appeared to be incomplete.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00224943
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  • 13
    Electronic Resource
    Electronic Resource
    Light and electron microscopic immunocytochemical localization of thyroglobulin in the thyroid gland of the anadromous sea lamprey, Petromyzon marinus L., during its upstream migration (1978)
    Springer
    Cell & tissue research 187 (1978), S. 473-478 
    Details
    ISSN: 1432-0878
    Keywords: Thyroid gland ; Immunocytochemistry ; Thyroglobulin ; Petromyzon marinus L
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Antibodies made against thyroglobulin (TG) were used in an immunocytochemical study for the light and electron microscopic localization of TG in the thyroid gland of the anadromous sea lamprey, Petromyzon marinus, during its upstream migration. TG was found in the follicular lumen and in some colloid droplets within the follicular cells. Except for an immunoreactive product observed in a small portion of the interstitial connective tissue, the location of TG in the lamprey was similar to that in the thyroid of the rat.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00229611
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  • 14
    Electronic Resource
    Electronic Resource
    Immunocytochemical study of the neurohypophysial hormone producing system of the lungfish, Protopterus aethiopicus (1978)
    Springer
    Cell & tissue research 190 (1978), S. 69-77 
    Details
    ISSN: 1432-0878
    Keywords: Hypothalamus ; Dipnoi (Protopterus) ; Neurohypophysial hormones ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Using the unlabeled antibody peroxidase-antiperoxidase complex (PAP) technique at the light microscopic level, it has been shown that, in the dipnoan preoptico-hypophysial neurosecretory system, vasotocin and mesotocin are synthesized in separate neurons. In the preoptic nuclei, the perikarya of these two types of neurosecretory neurons are not located preferentially. The two types of neurosecretory perikarya give rise to separate vasotocinergic and mesotocinergic axons, respectively. The dipnoan median eminence and neural lobe contain separate vasotocinergic and mesotocinergic nerve fibres, the general distribution of which is described. In the pars distalis and the pars intermedia of the hypophysis, neurohypophysial hormone-containing nerve fibres have not been found.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00210037
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  • 15
    Electronic Resource
    Electronic Resource
    Effect of cyproterone acetate, d-norgestrel and progesterone on cells of the pars distalis of the adenohypophysis in the beagle bitch (1978)
    Springer
    Cell & tissue research 191 (1978), S. 205-218 
    Details
    ISSN: 1432-0878
    Keywords: Cyproterone acetate ; d-Norgestrel ; Progesterone ; Pars distalis ; adenohypophysis ; Immunocytochemistry ; Dog (Beagle)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The effects of short-term (8 weeks) treatment with different doses of cyproterone acetate (CPA), d-norgestrel (d-N) and progesterone on cells of the pars distalis, as revealed by the immunoperoxidase technique, were studied in cycle-synchronized beagle bitches (first anoestrus). Pituitary glands from non-treated primiparous beagle bitches at the 6th and 9th week of pregnancy were also included. For immunochemical staining specific antisera to the following hormones were used: canine GH, canine PRL, porcine ACTH, bovine TSHβ, bovine LHβ and human FSHβ. Morphological features of high secretory activity in GH cells were evident even after the human oral contraceptive doses of CPA and d-N, and after a dose as low as 0.1 mg/kg/day subcutaneously (s.c.) of progesterone. In contrast, PRL cells did not show any significant treatment-related effects except in those animals which received the highest dose of d-N (0.5 mg/kg/day per os). In this group, as well as in all pregnant bitches, hyperplasia and hypertrophy of PRL cells were found. In the animals treated with the highest doses of CPA (4.0 mg/kg/day per os) and progesterone (42.5 mg/kg/day s.c.) as well as in pregnant bitches, ACTH/MSH and TSH cells showed marked atrophy and regressive changes. Similar morphological signs of depressed secretory activity were also observed in the cells shown to contain FSHβ and/or LHβ as a result of treatment with the highest dose of progesterone and at the 9th week of pregnancy. These structural responses indicate that quantitative and/or qualitative differences may exist between progesterone, the synthetic progesterone derivative CPA and the nortestosterone type progestagen d-N with regard to their effect on pituitary hormone secretion in the beagle bitch.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00222420
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  • 16
    Electronic Resource
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    Ultrastructural and cytochemical studies on the cytodifferentiation of duodenal endocrine cells (1978)
    Springer
    Cell & tissue research 194 (1978), S. 79-102 
    Details
    ISSN: 1432-0878
    Keywords: Duodenum ; Endocrine cells ; Differentiation ; Immunocytochemistry ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The development and cytodifferentiation of endocrine cells that produce the gastrointestinal hormones gastrin, cholecystokinin and secretin have been studied by a combined fluorescence-cytochemical, immunocytochemical and ultrastructural approach. The results show that, during development, several ultrastructurally distinct cell types exhibit COOH-terminal gastrin and cholecystokinin immunoreactivity. Furthermore, some cells simultaneously contain both gastrin- and cholecystokinin-specific antigenic determinants. Studies on the time course of development of gastrin and cholecystokinin cells, together with the above-mentioned data, suggest that gastrin cells may be converted into cholecystokinin cells in development. During this period, gastrin, cholecystokinin and secretin cells store the biogenic monoamine, 5-hydroxytryptamine a feature not displayed by the adult counter-parts of these cells. In the adult duodenum, characteristic enterochromaffin (EC) cells store 5-hydroxytryptamin for which, evidence for a possible hormonal role has been presented. Taken together, our data indicate that the differentiation of duodenal endocrine cells occurs in distinct steps, each involving a restriction in the biosynthetic repertoire of the cell.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00209235
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  • 17
    Electronic Resource
    Electronic Resource
    Distribution, ontogeny and ultrastructure of pancreatic polypeptide (PP) cells in the pancreas and gut of the chicken (1978)
    Springer
    Cell & tissue research 194 (1978), S. 377-386 
    Details
    ISSN: 1432-0878
    Keywords: Pancreas ; Gut ; Pancreatic polypeptide (PP) cells ; Immunocytochemistry ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Pancreatic polypeptide (PP) cells in the pancreas and gut of chickens were studied by immunocytochemistry. In the pancreas PP cells were numerous and disseminated in the exocrine parenchyma. In this location they were first seen at the 9th day of incubation, i.e. several days after the appearance of glucagon, insulin and somatostatin cells. Very large numbers of these cells occurred from about the 14th day until shortly after hatching when the PP cell frequency was somewhat reduced. At the 17th day of incubation PP cells appeared in the duodenum. Subsequently the number of PP cells in the duodenum increased, and PP cells began to appear also in the jejunum-ileum (19th day) and in the proventriculus and colon (21st day). At hatching and a few days thereafter, PP cells were relatively numerous in the small intestines but much less frequent in the proventriculus and colon. One week after hatching PP cells had disappeared from the colon but remained in the proventriculus and small intestines. Ultrastructurally the PP cell was clearly distinguishable from the insulin, glucagon and somatostatin cells. It was characterized by the presence of spherical cytoplasmic granules which were membrane-bound and moderately electron dense. Areas of firm adhesion between PP cells and acinar cells in the form of desmosomes and possibly also gap junctions were observed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00236159
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  • 18
    Electronic Resource
    Electronic Resource
    Identification by immunofluorescence of prolactin-and somatotropin-producing cells in the pituitary gland of the Mexican axolotl (Ambystoma mexicanum, Shaw) (1978)
    Springer
    Cell & tissue research 194 (1978), S. 497-501 
    Details
    ISSN: 1432-0878
    Keywords: Pituitary gland ; Mexican axolotl ; Prolactin ; Somatotropin ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The indirect immunofluorescence procedure was used to identify prolactin (LTH)-and somatotropin (STH)-producing cells in the pituitary of the Mexican axolotl. Histological staining techniques were employed to corroborate immunocytological results. The LTH cells are large, orange-staining cells (acidophils 1) distributed in the posterior two-thirds of the pars distalis. The STH cells are small, erythrosinophilic elements (acidophils 2) principally concentrated in the dorsal part of the pars distalis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00236168
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    Identification of the LH and TSH-secreting cells in the pituitary gland of the rhesus monkey (1978)
    Springer
    Cell & tissue research 190 (1978), S. 151-161 
    Details
    ISSN: 1432-0878
    Keywords: Pituitary gland ; Rhesus monkey ; Immunocytochemistry ; Luteinizing hormone ; Thyroid stimulating hormone
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Pituitary glands from juvenile (pre-pubertal) and adult male and female rhesus monkeys were examined following immunocytochemical staining with antisera to the beta subunits of ovine luteinizing hormone (LHβ) and of human thyroid stimulating hormone (TSHβ). The LHβ antiserum reacts with a cell that is PAS-positive, occurs singly and is randomly distributed throughout the pars distalis. The diameter of these cells is approximately 11.5 μm. They do not seem to vary in number in either juveniles (pre-pubertals) or adults, or in males or females. There appears to be fewer LH cells in the pituitary glands of pregnant and lactating females. In addition to staining cells in the pars distalis, the antiserum also reacts with a population of cells located in the pars tuberalis. The cells that stain with the anti-TSHβ serum are confined primarily to the pars distalis. They are approximately 15.8 μm in diameter and are generally found in groups or clusters located in the anterior and medial regions of the gland. The TSH cells vary in number from one animal to another; however, this variability is unrelated to the age or the sex of the animals. No demonstrable changes occur in the number of TSH cells during pregnancy or lactation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00210044
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  • 20
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    Effect of 17β-estradiol on cells stained for FSHβ and/or LHβ in the dog pituitary gland (1978)
    Springer
    Cell & tissue research 193 (1978), S. 211-218 
    Details
    ISSN: 1432-0878
    Keywords: 17β-estradiol ; Pituitary gland ; Dog (Beagle) ; Immunocytochemistry ; Glycoprotein hormones (FSH, LH, TSH)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The effect of long-term treatment (52 weeks) with high doses of 17β-estradiol (1.28 mg/kg/week intramuscularly) on gonadotrophs was studied in the pituitary gland of the beagle bitch. For immunochemical staining the immunoperoxidase technique and antisera to the specific beta (β) subunits of FSH and LH were employed. For control purposes antisera to the following hormones were also used: bovine TSHβ, canine GH, canine PRL and porcine ACTH1. In the pars distalis and pars tuberalis of control bitches, in addition to the cells which react solely with antisera to either LHβ or FSHβ, most cells were reactive to both antisera. The cells stained for FSHβ were less numerous than those shown to contain LHβ. TSHβ, PRL, GH and ACTH/MSH were localized in distinctly different cell types in the pars distalis of all control animals. In the treated bitches, almost complete regression of cells classically identified as gonadotrophs and stained for LHβ was observed. On the other hand, using the antiserum to FSHβ, selective immunochemical staining was localized in cells fitting the morphological characteristics of TSH cells. All these cells were also stained for TSHβ. However, a few cells were also shown to react solely with the antiserum to TSHβ. These cells, which seem to contain both TSHβ and FSHβ, were further clearly differentiated from PRL, GH and ACTH/MSH cells on the basis of their cytological features, intraglandular distribution and by immunochemical double staining. These observations support the concept that the one cell-one hormone theory may not necessarily apply to the glycoprotein hormones of the dog pituitary gland.
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    URL: http://dx.doi.org/10.1007/BF00209035
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  • 21
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    Immunocytochemical localisation of prolactin and growth hormone in the ovine pituitary (1978)
    Springer
    Cell & tissue research 194 (1978), S. 327-336 
    Details
    ISSN: 1432-0878
    Keywords: Prolactin ; Growth hormone ; Ovine pituitary ; Immunocytochemistry ; Granule size
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Using the peroxidase-antiperoxidase immunocytochemical staining technique, prolactin and growth hormone cells have been identified and described in the ovine pituitary. The image analysing computer, Quantimet 720, was used to assess accurately the size range of the secretory granules in these cell types. The area size distributions of the prolactin and growth hormone granules are similar. An increased proportion of larger granules was observed in the prolactin cells post-partum. Serial sections stained alternately for prolactin or growth hormone confirmed that the cells contain either prolactin or growth hormone but not both.
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    URL: http://dx.doi.org/10.1007/BF00220399
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  • 22
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    Immunocytochemical demonstration of somatostatin-containing neurons in the hypothalamus of the domestic mallard (1978)
    Springer
    Cell & tissue research 195 (1978), S. 183-187 
    Details
    ISSN: 1432-0878
    Keywords: Somatostatin-containing neurons ; Hypothalamus ; Median eminence ; Immunocytochemistry ; Domestic mallard
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In the hypothalamus of the adult domestic mallard, small to medium-sized perikarya are stained specifically with rabbit antiserum against cyclic somatostatin (PAP technique of Sternberger). The somatostatin-immunoreactive material is located in neurons different from those containing immunoreactive LHRH, vasotocin or mesotocin. Somatostatin-containing perikarya are observed 1) in a chain-like arrangement extending from the area of the median division of the supraoptic nucleus to the caudal end of the paraventricular nucleus; 2) as single cells in the preoptic region; and 3) as a conspicuous formation in the optic tract division of the supraoptic nucleus. In the rostral portion of the median eminence, somatostatin-immunoreactive axons penetrate into the external zone. Fine accessory fiber bundles project to the neural lobe.
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    URL: http://dx.doi.org/10.1007/BF00233685
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  • 23
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    β-lipotropin in brain: Localization in hypothalamic neurons by immunoperoxidase technique (1978)
    Springer
    Cell & tissue research 186 (1978), S. 393-398 
    Details
    ISSN: 1432-0878
    Keywords: β-Lipotropin ; Hypothalamus ; Hypophysial portal capillaries ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary β-Lipotropin (β-LPH) has been localized in hypothalamus and pituitary of sheep and ox by the immunoperoxidase technique. In both species β-LPH was found in perikarya of arcuate neurons as well as in cells of the anterior and intermediate lobes of the pituitary. A large number of immunoreactive axons were found in the arcuate region; some appeared to innervate other neurons and others projected to portal capillaries. Stained fiber segments were also scattered throughout the hypothalamus. The presence of β-LPH in hypothalamic neurons supports the possibility that brain β-LPH may be a precursor for opiate-like or other peptides which may be involved in neuromodulation or neurohormonal activities.
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    URL: http://dx.doi.org/10.1007/BF00224929
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  • 24
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    Beiträge zur automatisierten Spurenanalyse (1978)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 291 (1978), S. 20-26 
    Details
    ISSN: 1618-2650
    Keywords: Best. von Quecksilber in Biolog. Material, Umweltmaterial ; Spektralphotometrie, Atomabsorption ; AutoAnalyzer, Anreicherung an Silberwolle, ng-Bereich.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Zusammenfassung Ein modifiziertes automatisiertes Kaltdampf-AAS-Verfahren mit einem AutoAnalyzer II®-System wird beschrieben. Das Verfahren erlaubt sowohl die Bestimmung von anorganischem wie die von Gesamtquecksilber und erlaubt bis zu 120 Analysen/Tag. Die Anreicherung von Quecksilber als Amalgam an Silberwolle, gefolgt von rascher thermischer Zersetzung mit Hilfe einer Graphitküvette ermöglicht eine Nachweisgrenze von 0.01 ng/ml Probenlösung. Die relative Standardabweichung für homogenes Probenmaterial und das Gesamtverfahren ist ≤15%. Während der Analyse von Standard-Referenz-Proben wurden keine systematischen Abweichungen beobachtet. Der mittlere Gesamt-(2σ)fehler für eine einzelne Probe sollte daher 30% nicht überschreiten.
    Notes: Summary A modified automated cold vapour AAS procedure using an AutoAnalyzerII® system is described. The procedure permits the determination of inorganic as well as total mercury and enables to perform up to 120 analyses/day. The enrichment of mercury as amalgam on silver wool, followed by a fast thermal decomposition with the aid of a graphite cuvette enables a detection limit of 0.01 ng/ml sample solution. The relative standard deviation for homogeneous samples and the overall procedure is ≤15%. During the analysis of certified standard reference materials no systematic deviations were observed. The average total (2σ) error for a single sample should therefore not exceed 30%.
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    URL: http://dx.doi.org/10.1007/BF00468548
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    Flameless atomic absorption determination of beryllium in air filters (1978)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 291 (1978), S. 127-127 
    Details
    ISSN: 1618-2650
    Keywords: Best. von Beryllium in Luft ; Spektralphotometrie, Atomabsorption ; flammenlos
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00482552
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  • 26
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    Universelles Programm für die Auswertung von Extinktionen bei absorptionsspektroskopischen Meßverfahren mit einem Kleinrechner (1978)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 289 (1978), S. 17-23 
    Details
    ISSN: 1618-2650
    Keywords: Spektralphotometrie, Atomabsorption ; Auswertung, Rechnerprogramm
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Zusammenfassung Für die Auswertung der Messungen am Atomabsorptions-Spektrometer wurde ein Rechner-programm erarbeitet, das universell für die Berechnung von Analysenergebnissen nach der Bestimmung von Extinktionen verwendbar ist. Das Programm wird an einem Beispiel dargestellt.
    Notes: Summary For the calculation of analytical results a computer program is developed. It is an universal program for calculating concentrations with absorbance values of absorption methods, esp. for measurements in atomic-absorption spectroscopy.
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    URL: http://dx.doi.org/10.1007/BF00449233
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    Application of furnace AAS for the determination of the lanthanoids and the study of related phenomena (1978)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 289 (1978), S. 337-345 
    Details
    ISSN: 1618-2650
    Keywords: Best. von Lanthanoiden ; Spektralphotometrie, Atomabsorption ; Graphitrohr, pyrolyt. Graphit
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Summary The lanthanoids were determined by furnace AAS. The influence of coating the graphite tube with pyrolytic graphite is discussed and standard conditions and detection limits (10−8 to 10−12 g absolute) are presented. Resemblance of atomization with flame AAS is discussed and a proposal as to its mechanism is given.
    Notes: Zusammenfassung Der Einfluß einer Beschichtung der Graphitröhre mit pyrolytischem Graphit wird diskutiert und Standardbedingungen sowie Nachweisgrenzen (10−8 bis 10−12 g abs.) werden angegeben. Die Ähnlichkeit der Atomisierung bei der Flammen-AAS wird erörtert und der mögliche Mechanismus dargestellt.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00439724
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    Bestimmung von Arsen und Selen in Körperflüssigkeiten und Geweben mit der Hydrid-Methode (1978)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 290 (1978), S. 106-107 
    Details
    ISSN: 1618-2650
    Keywords: Best. von Arsen, Selen in Körperflüssigkeiten, Geweben ; Spektralphotometrie, Atomabsorption ; Hydrid-methode
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00482270
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    Eine einfache, schnelle und zuverlässige Bleibestimmung in Blut mittels Atomabsorptionsspektrometrie (1978)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 290 (1978), S. 108-109 
    Details
    ISSN: 1618-2650
    Keywords: Best. von Blei in Blut ; Spektralphotometrie, Atomabsorption ; Graphitrohr
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00482272
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  • 30
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    Richtigkeit bei der Bestimmung von Spurenelementen in Lebensmitteln mit der Graphitrohrtechnik AAS (1978)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 290 (1978), S. 144-145 
    Details
    ISSN: 1618-2650
    Keywords: Best. von Spurenelementen in Lebensmitteln ; Spektralphotometrie, Atomabsorption ; Graphitrohr, Richtigkeit
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00482303
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    Vergleich von Probenaufbereitungsverfahren beim quantitativen Nachweis von Schwermetallen in Fruchtsäften mit der flammenlosen Atomabsorptionsspektrometrie (1978)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 290 (1978), S. 145-146 
    Details
    ISSN: 1618-2650
    Keywords: Best. von Schwermetallen, Blei in Fruchtsäften ; Spektralphotometrie, Atomabsorption ; flammenlos, Vergleich von Aufschlußverfahren
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00482304
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    Determination of iron in ultrapure water by atomic absorption spectroscopy (1978)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 290 (1978), S. 289-291 
    Details
    ISSN: 1618-2650
    Keywords: Best. von Eisen in Wasser ; Spektralphotometrie, Atomabsorption ; hochreines Wasser, Anreicherung
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Summary Beside ionic impurities, DI water contains non-ionic compounds, which mainly consist of the oxyhydrates of the elements Fe, Ti, and Si present as colloids. This paper describes an analytical method for Fe, which is based on a simple enrichment by evaporation and the subsequent determination by flameless AAS. It is demonstrated that DI water systems, which are mainly based on coprecipitation and ion exchange, remove the Fe from the main water only to a small extent. The Fe content in DI water shows significant variations.
    Notes: Zusammenfassung Neben ionischen Bestandteilen enthält selbst hochreines Wasser auch nicht-ionische Komponenten, hauptsächlich Oxidhydrate der Elemente Ti, Fe und Si, die als Kolloide vorliegen. Die analytische Bestimmung des Eisens durch Eindampfen in einer einfachen Anordnung mit anschließender flammenloser AAS wird beschrieben. Es wird gezeigt, daß DI-Wasseranlagen, die heute zumeist noch nach dem Prinzip der Mitfällung und anschließendem Ionenaustausch arbeiten, nur begrenzt kolloidal vorliegendes Eisen entfernen und daß das Reinstwasser in seinem Fe-Gehalt stärkeren Schwankungen unterworfen ist.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00470129
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    Beitrag zur Multielement-Anreicherung aus Chrom (III)-salzen mit Hexamethylenammonium-hexamethylendithiocarbamidat (1978)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 290 (1978), S. 369-371 
    Details
    ISSN: 1618-2650
    Keywords: Best. von Elementspuren in Chrom(III)-salzen ; Spektralphotometrie, Atomabsorption ; Anreicherung an Aktivkohle mit Hilfe von Hexamethylenammonium-hexamethylendithiocarbamidat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Zusammenfassung Ein Verfahren wird beschrieben zur Anreicherung von 10 Elementen aus Chrom(III)-salzen durch nur einen Trennschritt. Als Reagens dient Hexamethylenammonium-hexamethylendithiocarbamidat; die Spurenverbindungen werden von Aktivkohle sorbiert. Nach Behandlung mit SalpetersÄure erhÄlt man eine praktisch chromfreie Lösung. Die Spuren werden durch Flammen-Atomabsorptionspektrometrie bestimmt.
    Notes: Summary A procedure is proposed for the enrichment of 10 elements from chromium(III) salts within only one separation step. Hexamethyleneammonium-hexamethylenedithiocarbamate is used as reagent and the trace compounds are sorbed on activated charcoal. Treatment with nitric acid yields a solution which is practically free from chromium. The traces are determined by flame atomic-absorption spectrometry.
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    URL: http://dx.doi.org/10.1007/BF00487381
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    Determination of lead in fruit juices after concentration using immobilized ED3A (1978)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 291 (1978), S. 128-128 
    Details
    ISSN: 1618-2650
    Keywords: Best. von Blei in Fruchtsäften ; Spektralphotometrie, Atomabsorption ; Konzentrierung mit immobil. ED3A
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00482554
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    Bestimmung von Beryllium in extraterrestrischer Materie mit Hilfe der flammenlosen Atomabsorptionsspektroskopie (1978)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 293 (1978), S. 410-411 
    Details
    ISSN: 1618-2650
    Keywords: Best. von Beryllium in extraterrestrischer Materie ; Spektralphotometrie, Atomabsorption ; Graphitrohrküvette
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00471462
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  • 36
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    Verwendung von hydrophilen Glykolmethacrylat-Gelen mit chemisch gebundenem 8-Hydroxychinolin bei Spurenanalysen (1978)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 292 (1978), S. 213-215 
    Details
    ISSN: 1618-2650
    Keywords: Verwendung von Chelatationsionenaustauschern zur Spurenanalyse ; Gele mit Hydroxychinolin. Best. von Kobalt, Eisen, Mangan, Nickel, Blei, Uran ; Spektralphotometrie, Atomabsorption ; Glykolmethacrylatgel
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Zusammenfassung Die Sorption wird bei einem Probelösung/Sorbens-Verhältnis von 103 ml/g ausgeführt, wozu die einfachste experimentelle Technik benutzt wird: 1 h Schütteln, Dekantieren, Zentrifugieren. Hohe Konzentrationen von Ammonium-, Alkali- und Erdalkalisalzen stören nicht. Mit Ausnahme von Co können alle gebundenen Metalle reversibel mit kleinen Säurevolumina statisch oder dynamisch eluiert werden. Die quantitativen Bestimmungen werden spektralphotometrisch (U) oder mit Hilfe der Atomabsorption (Co, Fe, Mn, Ni, Pb) ausgeführt.
    Notes: Summary The sorption is performed at a sample-sorbent ratio of 103 ml/g using the simplest experimental technique: shaking for 1 h, decantation, centrifugation. High concentrations of ammonium, alkali and alkaline earth salts do not interfere. All bound metals, except Co, can be reversibly eluted by small acid volumes in a batch or from a column. The eluted metals are quantitatively determined by spectrophotometry (U) or atomic absorption (Co, Fe, Mn, Ni, Pb).
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    URL: http://dx.doi.org/10.1007/BF00491810
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