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  • Immunocytochemistry  (17)
  • Elasticity
  • Rheology
  • Yeast
  • Springer  (21)
  • Air Force Geophysics Laboratory
  • Am. Geophys. Union
  • Amsterdam ; New York : North-Holland
  • Blackwell Publishing Ltd
  • Gordon and Breach Sc. Publ.
  • Inst. für Meteorologie und Geophysik der Johann Wolfgang von Goethe-Universität Frankfurt am Main
  • International Union of Crystallography
  • 1985-1989
  • 1975-1979  (21)
  • 1960-1964
  • 1977  (21)
Collection
Keywords
Publisher
  • Springer  (21)
  • Air Force Geophysics Laboratory
  • Am. Geophys. Union
  • Amsterdam ; New York : North-Holland
  • Blackwell Publishing Ltd
    • +
Years
  • 1985-1989
  • 1975-1979  (21)
  • 1960-1964
Year
  • 1
    Electronic Resource
    Electronic Resource
    Demonstration of enamel matrix proteins on root-analogue surfaces of rabbit permanent incisor teeth (1977)
    Springer
    Calcified tissue international 24 (1977), S. 223-229 
    Details
    ISSN: 1432-0827
    Keywords: Enamel-cementum-morphology ; Immunocytochemistry ; Biochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary The continuously erupting rabbit incisor tooth is normally thought of as having an enamel covered “crown” on its labial surface and a cementum covered “root” on its lingual surface. We have examined both surfaces of continuously erupting rabbit incisor teeth taken from near term embryos by a variety of means, including transmission and scanning electron microscopy, biochemical fractionation, and immunohistochemistry. In all cases, we could detect no qualitative difference in the early extracellular matrices taken from the labial and lingual surfaces of the teeth. Both matrices were shown to be composed of dentin and enamel, although the thickness and geometry of the enamel matrix on the lingual surface was somewhat different from that on the labial surface.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02223320
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  • 2
    Electronic Resource
    Electronic Resource
    Reversion by Fe(III) of the inhibition by hydroxamic acids of the cyanide-Insensitive respiration in the yeast Saccharomycopsis lipolytica (1977)
    Springer
    Archives of microbiology 114 (1977), S. 171-174 
    Details
    ISSN: 1432-072X
    Keywords: Iron ; Cyanide-resistance ; Hydroxamates ; Alternative respiration ; Yeast ; Saccharomycopsis lipolytica
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The specific inhibitory effect of benzhydroxamic acid on the cyanide-insensitive respiration could be reversed in whole cells of the yeast Saccharomycopsis lipolytica, by addition of Fe(III), in a way suggesting a competition between the added iron and an enzyme-bound metallic ion, both central atoms for the ligand benzhydroxamic acid. The possibility that added metal ions modify the penetration of BHAM into the cells was ruled out. Co(II), Cu(II) and Al(III) could substitute for Fe(III). A linear relation between the concentration in added Fe(III) and the reversed respiration rate was observed. At a given cell concentration. the reversion by added Fe(III) of the inhibitory effect of benzhydroxamic acid on the alternative respiration appeared more related to the degree of inhibition rather than to the concentration in added inhibitor. Increasing cell concentrations required increasing amounts of Fe(III) to reach the same level of reversion. No reversal occurred at concentrations in added Fe(III) lower than 0.1 mM, whatever the benzhydroxamic concentration, the cell concentration or the yeast batch.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00410780
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  • 3
    Electronic Resource
    Electronic Resource
    Location of mannan and chitin on thin sections of budding yeasts with gold markers (1977)
    Springer
    Archives of microbiology 115 (1977), S. 1-7 
    Details
    ISSN: 1432-072X
    Keywords: Cell walls ; Chitin ; Colloidal gold ; Concanavalin A ; Cytochemistry ; Mannan ; Wheat germ agglutinin ; Yeast ; Saccharomyces cerevisiae ; Candida utilis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Mannan was located on thin sections of Saccharomyces cerevisiae and Candida utilis with the homologous anti-mannan antibodies or with Concanavalin A, both labelled with gold granules. Fully synthesized mannan was found in the cell walls, on the plasmalemma and within the cytoplasm sometimes associated with vesicles and vacuoles. Chitin or its oligomers were located with wheat germ agglutinin in the bud scars but also in the cell wall and the cytoplasm near the plasmalemma. Both mannan and chitin or its oligomers were found in the forming septum and are synthesized within the cytoplasm. The gold method was also suitable for marking mannan and chitin simultaneously.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00427837
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  • 4
    Electronic Resource
    Electronic Resource
    Autoradiographic studies of 3H-dexamethasone uptake by immunocytochemically characterized cells of the rat pituitary (1977)
    Springer
    Cell & tissue research 182 (1977), S. 347-356 
    Details
    ISSN: 1432-0878
    Keywords: Pituitary ; Dexamethasone ; ACTH ; Autoradiography ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary 3H-Dexamethasone (10 μg/kg) was injected intravenously in adrenalectomized rats and after survival times of 5, 30, 60, and 180 min its uptake within the pituitary was studied by autoradiography. Radioactivity was concentrated in cell nuclei in the pars nervosa and pars distalis. Within the pars intermedia, only cells of the marginal zone were labeled. In the pars distalis, some cells showed a weak nuclear accumulation of radioactivity as early as 5 min after injection. The tissue radioactivity was nearly maximal at 5 min, and the proportion of radioactivity in nuclei reached a maximum of 60–70% by 30 min. In competition experiments, non-radioactive steroids (1 mg/kg) were injected 5 min before 3H-dexamethasone and sacrifice was 30 min later. Dexamethasone markedly diminished the nuclear accumulation in the pars distalis, but corticosterone and progesterone did not. In the pars nervosa, corticosterone and progesterone competed for nuclear uptake of 3H-dexamethasone, although less effectively than dexamethasone itself. Different cell types in the pars distalis were characterized by treating autoradiograms with an immuno-peroxidase bridge procedure. Cells treated with anti-ACTH 17–39 had the greatest nuclear concentration of radioactivity, and those stained with anti-TSH were least heavily labeled. Cells treated with antisera to GH, PRL, and hCG were moderately labeled.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00219770
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  • 5
    Electronic Resource
    Electronic Resource
    Isolation, characterization and localization of bovine adrenal medullary myosin (1977)
    Springer
    Cell & tissue research 178 (1977), S. 17-38 
    Details
    ISSN: 1432-0878
    Keywords: Myosin ; Immunocytochemistry ; Adrenal medulla ; Exocytosis ; Secretion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Myosin was isolated in high purity from the bovine adrenal medulla by gel filtration and ion exchange chromatography. The purified myosin was analyzed by electrophoresis in gels containing SDS and found to contain a 200,000 molecular weight heavy chain and major light chains of molecular weights 20,000 and 17,000 in a 1∶1∶1 molar ratio. At high ionic strength the myosin had high Ca-ATPase and K-EDTA-ATPase activities and low Mg-ATPase activity. At low ionic strength, the Mg-ATPase was activated to a low level by rabbit muscle actin. The myosin was found to decorate F-actin in the absence, but not the presence of ATP. In low ionic strength solutions, the myosin assembled into characteristic bipolar filaments. The distribution of this myosin in the adrenal medulla and of cross-reacting myosin in several other bovine tissues was determined with the use of antimedullary myosin immunoglobulin G as a specific stain that was detected by direct and indirect immunofluorescence. In the medulla strong staining was seen between the chords of chromaffin cells indicating the presence of a highly muscular vasculature that may perform functions analogous to those of the myoepithelium of exocrine glands. The chromaffin cells showed weak positive staining around the nuclei and in a pattern radiating toward adjacent blood vessels. Cells of the inner zone of the adrenal cortex showed strong staining in the peripheral cytoplasm while cells in the intermediate and outer zones did not stain. In a blood smear, platelets and the cytoplasm of leukocytes stained strongly while erythrocytes did not stain. In striated muscle and the gray and white matter of the cerebrum only the capillaries and larger vessels stained. In the liver the phagocytic cells bordering vascular sinuses stained strongly while the hepatocytes were separated from one another by a 2 micron trilaminar band possibly representing the microfilament web surrounding the bile canaliculi and associated with junctional complexes. The results suggest that myosin is present in several highly differentiated, non-motile tissue cells where it may play a role in secretion or other specialized functions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00232821
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  • 6
    Electronic Resource
    Electronic Resource
    Distribution, ontogeny and ultrastructure of somatostatin immunoreactive cells in the pancreas and gut (1977)
    Springer
    Cell & tissue research 185 (1977), S. 465-479 
    Details
    ISSN: 1432-0878
    Keywords: Somatostatin cells ; Pancreas ; Gut ; Immunocytochemistry ; Comparative study
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Somatostatin cells are numerous in the pancreas and digestive tract of mammals as well as birds. In the pancreas of chicken, cat and dog they occur in both the exocrine parenchyma and in the islets. In the rat and rabbit, somatostatin cells have a peripheral location in the islets, whereas in the cat, dog and man the cells are usually more randomly distributed. In the stomach of rabbits and pigs, somatostatin cells are more numerous in the oxyntic gland area than in the pyloric gland area, whereas the reverse is true for the cat, dog and man. In the cat, pig and man, somatostatin cells are fairly numerous in the duodenum, whereas in the rat, rabbit and dog they are few in this location. In the remainder of the intestines somatostatin cells are few but regularly observed. Somatostatin cells are numerous in the human fetal pancreas and gut. In the fetal rat, somatostatin cells first appear in the pancreas and duodenum (at about the 16–17th day of gestation) and subsequently in the remainder of the intestine. Somatostatin cells do not appear in the gastric mucosa until after birth. Three weeks after birth, somatostatin cells show the adult frequency of occurrence and pattern of distribution. In the chicken, somatostatin cells are numerous in the proventriculus, absent from the gizzard, abundant in the gizzard-duodenal junction (antrum), infrequent in the duodenum and virtually absent from the remainder of the intestines. No immunoreactive cells can be observed in the thyroid of any species nor in the ultimobranchial gland of the chicken. In the chick embryo, somatostatin cells are first detected in the pancreas and proventriculus (at about the 12th day of incubation). They appear in the remainder of the gut much later, in the duodenum at the 16th day, in the antrum at about the 19th day and still later in the lower small intestine. The ultrastructure of the somatostatin cells was studied in the chicken, rat, cat and man; the cells were identified by the consecutive semithin/ultrathin section technique. The somatostatin cells display the properties of the D cell. There was no difference in granule ultrastructure between somatostatin cells in the gut and the pancreas. The granules, which are the storage site of the peptide, are round, supplied with a tightly fitting membrane and have a moderately electron-dense, fine-granulated core. The mean diameter of the somatostatin granules is smallest in rat (155–170 nm) and largest in the chicken (270–290 nm).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00220651
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  • 7
    Electronic Resource
    Electronic Resource
    Elastic and strength properties of canine long bones (1977)
    Springer
    Medical & biological engineering & computing 15 (1977), S. 72-74 
    Details
    ISSN: 1741-0444
    Keywords: Bone ; Elasticity ; Mechanical strength
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Summary The mechanical properties of canine long bones have been determined. The mean and standard deviation of the maximum load supported by six fresh canine femurs in 3-point bending was 1366±253 N. In all cases, the bones sustained a considerable amount of plastic deformation before failure. The modulus of elasticity (GN/m2), ultimate tensile stress (MN/m2) and yield stress (MN/m2) were 13·86, 108·3 and 88·3 for six humeral specimens and 13·88, 142·1 and 104·0 for 13 tibial specimens, respectively. The tibial specimens showed a statistically significant higher ultimate strength than the humeral specimens.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02441578
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  • 8
    Electronic Resource
    Electronic Resource
    Hydrodynamics of blood flow through the inferior vena cava (1977)
    Springer
    Medical & biological engineering & computing 15 (1977), S. 248-253 
    Details
    ISSN: 1741-0444
    Keywords: Elasticity ; Haemodynamics ; Inferior vena cava ; Negative-resistance effects
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Description / Table of Contents: Sommaire La veine cave inférieure est un vaisseau affaissable soumis á des variations de la pression de respiration. Les observations cliniques sur le corps humain démontrent que, au cours d'une aspiration profonde, l'accroissement de la pente de pression propulsive provoque l'affaissement de la veine cave inférieure près du diaphragme, et réduit paradoxalement la circulation sanguine. Les empreintes faites au cours des autopsies démontrent qu'il se produit une constriction élastique fortement localisée à l'endroit où la veine cave inférieure, traverse le diaphragme. Un modèle mécanique passif, comportant une constriction élastique semblable, présente le même comportement paradoxal de résistance négative, conformément aux principes de l'écoulement à travers des tubes affaissables.
    Abstract: Zusammenfassung Die Vena cava inferior ist ein kollabierendes Gefäss, das Respirationsdruckschwankungen unterliegt. Klinische Beobachtungen an Menschen zeigen, dass ein Anstieg in dem propulsiven Druckgefälle bewirkt, dass die untere Hohlvene nahe der Membrane kollabiert und paradoxerweise daher den Blutstrom reduziert. Postmortale Abbildungengen zeigen, dass eine stark lokalisierte elastiche Verengung daher den Blutstrom reduziert. Postmortale Abbildungen zeigen, dasse eine stark lokalisierte elastische Verengung eintritt, wo die untere Hohlvene durch die Membrane führt. Ein passives mechanisches Modell mit einer ähnlichen elastischen Verengung zeigt ein ähnliches paradoxes oder negatives Widerstands-Verhalten, das den Prinzipien über den Strom durch kollabierende-Gefässe entspricht.
    Notes: Abstract The inferior vena cava (i.v.c.) is a collapsible vessel which is subject to respiratory pressure variations. Clinical observations on humans show that, during deep inspiration, an increase in the propulsive pressure gradient causes the i.v.c. to collapse near the diaphragm and paradoxically reduces the blood flow. Postmortem casts show that there is a sharply localised elastic constriction where the i.v.c. passes through the diaphragm. A passive mechanical model, incorporating a similar elastic constriction, exhibits similar paradoxical or negative-resistance behaviour, which is in accordance with the principles governing flow through collapsible tubes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02441045
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  • 9
    Electronic Resource
    Electronic Resource
    Immunocytochemical demonstration of the hypothalamo-hypophysial vasotocinergic system of Lampetra fluviatilis (1977)
    Springer
    Cell & tissue research 177 (1977), S. 317-323 
    Details
    ISSN: 1432-0878
    Keywords: Hypothalamus ; Lamprey ; Vasotocinergic system ; Adenohypophysis ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary With the use of the unlabeled antibody peroxidase-antiperoxidase (PAP) technique at the light microscopic level, it was shown that the preoptico-hypophysial neurosecretory system of the adult migrating Lampetra fluviatilis is a vasotocinergic system. It does not synthesize vasopressin. The results are entirely consistent with earlier chromatographic and pharmacological indications that it produces little or no oxytocin-like peptide hormone. In the adenohypophysis, immunoreactive neurohypophysial peptidergic fibres are absent.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00220307
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  • 10
    Electronic Resource
    Electronic Resource
    Electron microscopic immunocytochemical demonstration of separate mesotocinergic and vasotocinergic nerve fibres in the pars intermedia of the amphibian hypophysis (1977)
    Springer
    Cell & tissue research 178 (1977), S. 175-181 
    Details
    ISSN: 1432-0878
    Keywords: Pars intermedia of hypophysis ; Rana temporaria ; Mesotocinergic and vasotocinergic fibres ; Immunocytochemistry ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary With the use of the unlabeled antibody peroxidase-antiperoxidase (PAP) method at the electron microscopic level, it has been shown that the pars intermedia of the hypophysis of Rana temporaria contains a diffuse intercellular network of separate mesotocinergic and vasotocinergic nerve fibres.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00219045
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