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  • Articles  (31)
  • Scanning electron microscopy  (31)
  • Springer  (31)
  • American Physical Society (APS)
  • Blackwell Publishing Ltd
  • 1975-1979  (31)
  • 1979  (14)
  • 1977  (17)
Collection
  • Articles  (31)
Source
Keywords
Publisher
  • Springer  (31)
  • American Physical Society (APS)
  • Blackwell Publishing Ltd
Years
  • 1975-1979  (31)
Year
Topic
  • 1
    Electronic Resource
    Electronic Resource
    SEM analysis of amphibian mesodermal migration (1977)
    Springer
    Development genes and evolution 181 (1977), S. 31-40 
    Details
    ISSN: 1432-041X
    Keywords: Cell migration ; Mesoderm ; Gastrulation ; Scanning electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary At the end of gastrulation, the lateral mesoderm of amphibian embryos migrates ventrally between the ectoderm and the endoderm. The present study is an examination of the morphology of the leading cells of the mesodermal sheet and of the substratum over which they move (the inner surface of the ectoderm). The cells of the leading edge of the mesoderm are generally round, with very short and narrow flattened projections in the forward direction. These projections do not have a “ruffled” morphology, regardless of whether fixation is carried out before or after the ectoderm and mesoderm are dissected away from the endoderm. The inner surface of the ectoderm is covered with fine (450–500A) filamentous extracellular material and the ectoderm cells sometimes extend cytoplasmic processes (approx. 0.1 μ wide) onto the leading surface of the mesoderm or onto adjacent ectoderm cells. These studies indicate that the morphology of cell migration in amphibians is closer to that seen inFundulus than to that characteristic of chick or mammalian cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00857266
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  • 2
    Electronic Resource
    Electronic Resource
    Growth of embryonic chick tibiae in ovo and in vitro: A scanning electron microscope study (1979)
    Springer
    Calcified tissue international 27 (1979), S. 33-40 
    Details
    ISSN: 1432-0827
    Keywords: Chick embryo ; Bone ; Organ culture ; Scanning electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary The study describes the ultrastructure of the mineralized portion of chick tibiae from 10 days in ovo to 2 days post-hatch. At 10 days a single mineralized cylinder surrounds the diaphysis. On its outer surface columnar trabeculae join to form ridges parallel to the long axis of the bone. These ridges are covered by another cylinder and form the haversian canals. At 11 days vascular invasion of the marrow cavity occurs and resorption of the endosteal surface begins. This type of periosteal deposition and endosteal resorption is repeated during and subsequent to embryonic development. The mineralized portion of 10-day chick tibiae cultured for 2 days in modified BGJ medium was compared with 10-, 11-, and 12-day tibiae in ovo. Cultured tibiae were similar in length and calcium content to 11-day tibiae in ovo. The form of mineral deposited in ovo and in culture was the same, namely, aggregates of spherical mineral clusters. Differences in culture included the following: (a) few concentric cylinders were deposited as compared with tibiae in ovo; (b) trabeculae were not arranged in rows and ridges in culture; (c) osteocytic lacunae were restricted to bases of trabeculae rather than uniformly distributed as in ovo; and (d) the endosteal surface of tibiae in culture appeared etched.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02441158
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  • 3
    Electronic Resource
    Electronic Resource
    Structure and association of wall fibrils produced by regenerating tobacco protoplasts (1979)
    Springer
    Planta 146 (1979), S. 203-210 
    Details
    ISSN: 1432-2048
    Keywords: Cellulose ; Microfibrils ; Negative staining ; Nicotiana ; Protoplasts ; Scanning electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A study has been made of the wall fibrils produced by tobacco protoplasts, using scanning electron microscopy in conjunction with negative staining. It has been shown that the fibres seen in scanning electron microscopy correspond to aggregates of microfibrils. These aggregates are only visible where they are lifted clear of the protoplast surface. Negative staining of fixed protoplasts shows that the aggregation of microfibrils into the fibres visible in scanning electron microscopy is probably produced by air-drying. Gentle disruption of microfibrils produces both random broken fragments and bundles of short pieces of fibrillar material about 60 nm in length. This material is present in undisrupted young walls, but not in undisrupted older walls. The microfibrils in young walls seem much more fragile and liable to breakage than those in older walls. These results are discussed in terms of the interpretation of scanning electron microscope images and the mechanism of cellulose microfibril formation by higher plants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00388233
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  • 4
    Electronic Resource
    Electronic Resource
    Localization of α-galactomannan on the surface of Schizosaccharomyces pombe cells by scanning electron microscopy (1977)
    Springer
    Archives of microbiology 112 (1977), S. 123-126 
    Details
    ISSN: 1432-072X
    Keywords: Bandeiraea simplicifolia ; Schizosaccharomyces pombe ; Colloidal gold ; Cytochemistry ; α-Galactomannan-lectin ; Scanning electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Galactomannan was localized by scanning and transmission electron microscopy on the cells and cell walls of Schizosaccharomyces pombe. The markers were prepared from colloidal gold granules labelled with an α-galactopyranosyl-binding lectin isolated from the seeds of Bandeiraea simplicifolia. Part or all of this α-galactomannan was present in the outer layer of the cell wall and was uniformly distributed even on the fission scars.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00429323
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  • 5
    Electronic Resource
    Electronic Resource
    Scanning electron microscopy of dissociated pancreatic acinar cell surfaces (1977)
    Springer
    Cell & tissue research 176 (1977), S. 493-504 
    Details
    ISSN: 1432-0878
    Keywords: Pancreas ; Acinar cells ; Cell surface ; Dissociation ; Scanning electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The pancreatic acinar cell surfaces have been studied by SEM with a dissection technique and correlated with results obtained by TEM. The SEM results demonstrate characteristic arrangement of microplicae which in some areas are densely packed. In many areas, the microplicae are distributed in such a manner that they create zones with typical geometrical shapes and show a relatively smooth surface. These smooth areas may coincide, as indicated by correlated TEM results, with the limits of intimate contact between adjacent acinar cells which, in turn, represent part of the junctional complex. Another aspect revealed by these SEM preparations concerns the presence of groups of densely packed microplicae, arranged in regular rows and distributed along some grooves and/or infoldings of the cellular surface. On the basis of SEM and TEM information, it is likely that these structures correspond to intercellular (and possibly, in some cases, intracellular) canaliculi which topographically form a kind of extensive microlabyrinthine arrangement running along all the cell sides. One final point revealed by fractured samples concerns the finding of spherical zymogen droplets within the vesicles of the Golgi complex. Because in many scanning images these vesicles appear connected by small openings, it is suggested that they may represent a system of intercommunicating chambers (vacuoles) through which the zymogen droplets can be continuously accumulated and discharged into the acinar lumen.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00231404
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  • 6
    Electronic Resource
    Electronic Resource
    The third ventricle of monkeys (1977)
    Springer
    Cell & tissue research 177 (1977), S. 307-316 
    Details
    ISSN: 1432-0878
    Keywords: Third ventricle ; Mature monkeys ; Scanning electron microscopy ; Ependyma
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Surface features of the ependymal lining of the third ventricle in mature male and female monkeys have been investigated with scanning electron microscopy (SEM). Broad aspects of third ventricular morphology from three species of monkey are similar regardless of sex. The lateral walls are heavily ciliated whereas the ventral floor and most ventral parts of the lateral walls are not. Clumps of cilia on the lateral walls are so dense that underlying surface details are usually obscured. There is a transition zone between the ciliated lateral wall and nonciliated ventral floor. The floor and lower part of the lateral walls of the third ventricle exhibit a characteristic polygonal pattern upon which surface specializations such as microvilli, blebs and polymorphous membrane protrusions are superimposed. Ependyma of the choroid plexus of the third ventricle also display membrane specializations. Supraependymal cells are more visible in nonciliated regions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00220306
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  • 7
    Electronic Resource
    Electronic Resource
    Three-dimensional development of luminal projections and junctional complexes in the developing central nervous system blood vessels of the rat (1977)
    Springer
    Cell & tissue research 175 (1977), S. 541-549 
    Details
    ISSN: 1432-0878
    Keywords: Blood vessels ; High voltage electron microscopy ; Scanning electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The luminal surface features and Junctional complexes from developing blood vessels in the rat central nervous system have been studied by high-voltage electron microscopy and scanning electron microscopy. Developing blood vessels exhibit three types of luminal projections; marginal folds or ridges at Junctional complexes, ridges not at Junctional complexes and microvilli. Both types of ridges are associated with troughs or depressions in the luminal surface of the endothelial cell. Those ridges not associated with Junctional complexes take part in the production of enclosed tunnels in the endothelial cell cytoplasm. Fusion of the external leaflets of Junctional complexes between adjacent endothelial cells occurred, initially, near the luminal surface of the blood vessel with other small fusion sites forming in the direction of the basal lamina secondarily. Further fusion activity to produce the zonula occludens type junction appeared to spread outwards from the smaller fusion sites.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00222417
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  • 8
    Electronic Resource
    Electronic Resource
    Classification of human placental villi (1979)
    Springer
    Cell & tissue research 200 (1979), S. 409-423 
    Details
    ISSN: 1432-0878
    Keywords: Human placenta ; Classification of villi ; Histology ; Scanning electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The classification of human placental villi was reviewed on the basis of material prepared by means of special methods. The material from in situ normal-term placentae was biopsied by aspiration into glutaraldehyde. The classification was made on the basis of light-microscopic observations of semithin sections, reconstructions from serial sections, and scanning-electron micrographs. The peripheral villous tree is roughly divided into stem (ramuli), intermediate and terminal villi. The intermediate villi may be further subdivided as mature and immature types, which are found between the stem and terminal villi. Some of the terminal villi possess a local specialization described as the neck region. The histological characteristics and the branching pattern of each type are described, and the basis of the proposed classification is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00234852
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  • 9
    Electronic Resource
    Electronic Resource
    Photoreceptor-like outer segments in the pineal organ of the lovebird, Uroloncha domestica (Aves: Passeriformes) (1977)
    Springer
    Cell & tissue research 182 (1977), S. 139-143 
    Details
    ISSN: 1432-0878
    Keywords: Pineal organ ; Uroloncha domestica (Aves, Passeriformes) ; Photoreceptor-like cells ; Scanning electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In the pineal organ of the lovebird, Uroloncha domestica, bulbous, cup-shaped and elongated outer segments of photoreceptor-like pinealocytes are demonstrated by scanning electron microscopy. These scarce outer segments, 4–11 μm in length, extend into the pineal lumen. The present structural observations speak in favor of photosensitive pinealocytes in the pineal organ of Uroloncha domestica. The relation of the photoreceptor-like pinealocytes to acetylcholinesterase-positive nerve cells and a nervous connection between the pineal and the brain indicate that the pineal organ of this passeriform species may be the site of neuroendocrine and photoreceptive functions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00222062
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  • 10
    Electronic Resource
    Electronic Resource
    Scanning electron microscopy of peripheral blood leukocytes of the chicken (1979)
    Springer
    Cell & tissue research 204 (1979), S. 147-153 
    Details
    ISSN: 1432-0878
    Keywords: Granulocytes ; Lymphocytes ; Monocytes ; Scanning electron microscopy ; Chicken
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Polymorphonuclear leukocytes, e.g., neutrophilic granulocytes, were enriched from heparinized blood by a Ficoll-step-gradient centrifugation procedure. Scanning electron microscopy (SEM) revealed a surface morphology of narrow ridge-like profiles and small ruffles with occasional microprocesses. Mononuclear leukocytes were isolated by centrifugation over a Ficoll-Metrizoat gradient. The lymphocytes showed varying numbers of microvilli of different length, size and shape. B lymphocytes, characterized by their capability of “sheep red blood cell (SRBC)-rosette formation”, displayed a similar surface morphology. Completely smooth lymphocytes, described in the literature as T lymphocytes, could not be detected, although many lymphocytes with few microprocesses were observed. Thus, SEM is not a useful tool for distinguishing between B and T lymphocytes in the peripheral blood of chickens. Monocytes were characterized by prominent membrane-like ruffles, but in some cases they closely resembled granulocytes. An influence of the various separation media on the surface morphology of the isolated cells could not be detected when compared with cells isolated by the buffy-coat method.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00235171
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