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  • Articles  (31)
  • Growth  (31)
  • Springer  (31)
  • American Physical Society (APS)
  • Blackwell Publishing Ltd
  • 1975-1979  (31)
  • 1979  (22)
  • 1977  (9)
Collection
  • Articles  (31)
Publisher
  • Springer  (31)
  • American Physical Society (APS)
  • Blackwell Publishing Ltd
Years
  • 1975-1979  (31)
Year
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Calcified tissue international 23 (1977), S. 161-170 
    ISSN: 1432-0827
    Keywords: Bone ; Growth ; Rat ; Remodeling ; Tetracyclines
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary Cortical bone remodeling along the femur diaphysis was determined in normal female rats (Sprague-Dawley) with the tetracycline technique. Three segments on the cortical bone circumference (the anterolateral, the medial, and the posterior) were found to be most suitable for the study of the remodeling process. Oxytetracycline was administered at age 60 and 75 days, and groups of animals were killed at age 75, 85, 95, and 105 days. The accumulated endosteal growth during age 60 to 75 days in the anterolateral segment was found to increase uniformly in the distal direction along the femur diaphysis. A method is described where this accumulated endosteal growth is used. This method eliminates the use of calipers to determine the section level and makes it possible to study comparable sections even after varying periods of time. The proximal part of the diaphysis showed periosteal apposition in all three segments. The periosteal apposition turned into resorption in the distal part of the diaphysis in the anterolateral and medial segments, whereas the periosteal appsition increased in the posterior segment. The endosteal growth increased in the distal direction in the anterolateral and medial segments. Irregular OTC bands made measurements of endosteal remodeling in the posterior segment impossible. The cortical width decreased in the distal direction along the femoral shaft. Comparison between the different age groups is described and also the relation between the accumulated endosteal growth and the diameter of the medullary cavity.
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Development genes and evolution 183 (1977), S. 107-117 
    ISSN: 1432-041X
    Keywords: RNA polymerases ; Enzyme localization ; Isolated nuclei ; Growth ; Differentiation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Isolated nuclei ofPhysarum contain endogenous RNA polymerase activity. We provide evidence for four different states of RNA polymerase B: 1. free enzyme (85%); 2. weakly bound enzyme (10%) and 3. tightly bound enzyme (0–4%), which can be solubilized from isolated nuclei with 0.5 M and 1.5 M NaCl respectively; 4. “initiated” enzyme. The latter fraction (1–5% of the total RNA polymerase B) is not soluble in salt extractions, does not accept external templates, shows high salt optimum for transcription (0.4 M NaCl) and produces by elongation RNA molecules of mainly 10 S. Treatment of isolated nuclei from differentiating cultures with Triton X-100 increases the proportion of the “initiated” enzyme at the expense of the tightly bound enzyme fraction. This indicates a potential transcription control mechanism which operates at the chromatin level and results in variable proportions of silent and transcribing RNA polymerase B molecules during differentiation of Physarum.
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Planta 147 (1979), S. 43-49 
    ISSN: 1432-2048
    Keywords: Growth ; Roots ; Shoots ; Solute accumulation ; Water stress ; Zea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Potted maize seedlings were subjected to a single period of water stress. As the severity of water stress increased, measurements were made of leaf and root solute and water potentials, leaf diffusive conductance and leaf and root growth. After day four of the drying cycle, the rate of leaf extension and the development of leaf area were reduced. This reduction correlated well with a reduction in leaf turgor which occurred at this time. A significant accumulation of solutes in the root tips of the unwatered plants resulted in the maintenance of root turgor for the duration of the water stress treatment. Root growth of the unwatered plants was also maintained as the severity of water stress increased. A mild degree of water stress resulted in a net increase in root growth compared to the situation in well-watered plants. The significance of solute regulation and continued root growth for plants growing in drying soil is discussed.
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Planta 146 (1979), S. 363-368 
    ISSN: 1432-2048
    Keywords: Cell walls ; Gibberellin ; Growth ; Lactuca
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Radioactive 2,000 g pellets (2KP) derived from lettuce (Lactuca sativa) hypocotyl sections incubated with [3H]gibberellin A1 ([3H]GA1) were subjected to a range of treatments to test the stability of labelling. The labelled association was stable in 0.1 M buffer at pH 3 and pH 9 and also in 1 M salt, organic solvents, protease or cellulase. Both 1 M potassium hydroxide (KOH) and a quaternary ammonium hydroxide tissue solubiliser effected an 80% release of incorporated [3H]. No radioactivity was released during polyacrylamide gel electrophoresis. Gel chromatography of KOH digests indicated the presence of labelled compounds with a higher molecular weight than GA1. This material remained near to the origin during high-voltage paper electrophoresis. Calculations based upon KOH extraction of 2KP material suggested that 5 to 20% of the total uptake of [3H]GA1 could be recovered from this fraction. The implications of these findings are discussed.
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Planta 146 (1979), S. 405-408 
    ISSN: 1432-2048
    Keywords: Auxin ; Growth ; Root growth ; Zea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The endogenous indol-3yl-acetic acid (IAA) of detipped apical segments from roots of maize (cv ORLA) was greatly reduced by an exodiffusion technique which depended upon the preferential acropetal transport of the phytohormone into buffered agar. When IAA was applied to the basal cut ends of freshly prepared root segments only growth inhibitions were demonstrable but after the endogenous auxin concentration had been reduced by the exodiffusion technique it became possible to stimulate growth by IAA application. The implications of the interaction between exogenous and endogenous IAA in the control of root segment growth are discussed with special reference to the role of endogenous IAA in the regulation of root growth and geotropism.
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  • 6
    ISSN: 1432-2048
    Keywords: Gibberellin ; Growth ; Lactuca ; Protein-synthesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The relationship between protein synthesis and the incorporation of [3H]gibberellin A1 ([3H]GA1) into a 2,000xg pelletable (2KP) fraction from lettuce (Lactuca sativa L.) hypocotyl sections has been investigated. Concentrations of D-2-(4-methyl-2,6-dinitroanilino)-N-methylpropionamide (MDMP) between 10-7 M and 10-4 M caused increasing inhibition of growth, 2KP labelling and incorporation of [14C]leucine into soluble protein. Growth and 2KP radioactivity were highly correlated (r=0.996). Transfer to MDMP early or late in the course of GA response caused reductions in both growth and incorporation into the 2KP fraction. Exposure to the inhibitor had more effect at 4 h than at 20 h. The proportions of alkali-soluble and insoluble radioactivity in the 2KP fraction were also altered by this treatment. The implications of these findings are discussed.
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  • 7
    ISSN: 1432-2048
    Keywords: Avena coleoptile segments ; Growth ; Indoleacetic acid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The intial phases of auxin-induced growth in coleoptile segments of Avena sativa L. were investigated using a high resolution growth recording technique, based on an angular position sensing transducer. The first response to the hormone is a slight, transient reduction of the growth rate lasting about 5 min. After this phase growth rate increases to a maximum. The duration of the increase and the maximum clearly depend on the concentration of the hormone. With increasing auxin concentration the duration of the growth rate increase is reduced from about 80 min in 10-9 M indoleacetic acid (IAA) to about 14 min in 10-4 M IAA. After the maximum the growth rate declines. Looking at the maximum of the growth rate, we obtained a dose-response curve with a sharp increase between 10-9 M and 10-6 M IAA and a slight decline between 10-6 M and 10-4 M IAA. This result is confirmed by growth rates measured one and two hours after the application of the hormone.
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  • 8
    ISSN: 1432-072X
    Keywords: Methane oxidizing bacteria ; Growth ; Excretion ; Cell cycle control
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The growth rates of the methane oxidizing strain M 102 were unstable. In unlimited batch cultures they ranged from 0 to 1.4 d-1 with most frequent values around 0.25 d-1. The yield coefficients were between 0.1 and 1.6 mg dry weight per mg methane and varied from 1.1 to 0.4 even in a continuous culture with the limiting substrate in the gas phase. It was possible to prove intermitten release and uptake of organic compounds by the definition of a “net” yield coefficient and its theoretical boundaries. The response of the fluctuations to varying dilution frequencies and culture volumes showed analogies to controlled systems. The simultaneous variations in the age distribution and in the excretion pattern revealed the dependence of the dividing stages on a product of the young cells. The mathematical description of the findings suggests that the young cells favor division at their own disadvantage. The consequent autonomous synchronisations are the reason for the unstable growth rates of strain M 102.
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  • 9
    ISSN: 1432-072X
    Keywords: Neurospora crassa ; Adenylyl cyclase ; Crips mutant ; Nutritional deficiencies ; Growth ; Fungi ; Cyclic AMP
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The inability to synthesize cyclic AMP drastically affects the nutritional metabolism of Neurospora crassa. The adenylyl cyclase-less mutant cr-1 (crisp) did not utilize several carbon sources, including glycerol, mannitol, arabinose, and casaminoacids. However, in glucose or acetate it grew as well as the wild type. The following evidence suggested that these nutritional deficiencies were a direct result of the cr-1 mutation: (i), in crosses to wild type they segregated together with the crisp morphological marker; (ii), cyclic AMP added to the cr-1 mutant growth medium overcame the nutritional deficiencies; (iii), the cyclic AMP effect was specific for the crisp mutant, for it was not observed with the wild type, nor with a spontaneous glycerolutilizing cr-1 strain.
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 121 (1979), S. 91-96 
    ISSN: 1432-072X
    Keywords: Phosphate inhibition ; Cyclic AMP ; Antibiotic biosynthesis ; Secondary metabolism ; Growth ; Protein synthesis ; Turmycin ; Streptomyces hygroscopicus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Inorganic phosphate inhibited the biosynthesis of the macrolide antibiotic turimycin in different strains of Streptomyces hygroscopicus. In the wild type strain a depression was observed with increasing phosphate concentrations. A total inhibition was found at 0.1 M phosphate. In a high producing mutant a minimum of turimycin production occured when the phosphate concentration was between 5 mM and 10 mM. Above this concentration the antibiotic synthesis increased again but the production period shifted to a later period of cultivation. Addition of inorganic phosphate resulted in an initial increase of intracellular cyclic AMP content. But a second elevation characterizing the normal level of cyclic AMP throughout the growth phase was prevented by phosphate. Exogenous cyclic AMP as well as positive effectors of the adenylyl cyclase system were able to overcome the phosphate suppression. Cyclic AMP abolished the reduction of protein synthesis following phosphate addition and caused the reappearance of a protein band which may be responsible for the turimycin biosynthesis.
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