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  • Spektralphotometrie, Atomabsorption  (36)
  • Immunocytochemistry  (34)
  • Springer  (70)
  • 1975-1979  (70)
  • 1978  (36)
  • 1977  (34)
Collection
Keywords
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  • Springer  (70)
Years
  • 1975-1979  (70)
Year
  • 1
    Electronic Resource
    Electronic Resource
    Demonstration of enamel matrix proteins on root-analogue surfaces of rabbit permanent incisor teeth (1977)
    Springer
    Calcified tissue international 24 (1977), S. 223-229 
    Details
    ISSN: 1432-0827
    Keywords: Enamel-cementum-morphology ; Immunocytochemistry ; Biochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary The continuously erupting rabbit incisor tooth is normally thought of as having an enamel covered “crown” on its labial surface and a cementum covered “root” on its lingual surface. We have examined both surfaces of continuously erupting rabbit incisor teeth taken from near term embryos by a variety of means, including transmission and scanning electron microscopy, biochemical fractionation, and immunohistochemistry. In all cases, we could detect no qualitative difference in the early extracellular matrices taken from the labial and lingual surfaces of the teeth. Both matrices were shown to be composed of dentin and enamel, although the thickness and geometry of the enamel matrix on the lingual surface was somewhat different from that on the labial surface.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02223320
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  • 2
    Electronic Resource
    Electronic Resource
    Phytochrome photoreversibility: Empirical test of the hypothesis that it varies as a consequence of pigment compartmentation (1978)
    Springer
    Planta 141 (1978), S. 129-134 
    Details
    ISSN: 1432-2048
    Keywords: Avena ; Immunocytochemistry ; Phytochrome
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Phytochrome of oat (Avena sativa L., cv. Garry) coleoptile cells in the red-light-absorbing form, Pr, is diffusely distributed while after conversion to the far-red-light-absorbing form, Pfr, it is observed only in very small areas within the cell. Comparison of phytochrome photoversibility measurements to the distribution of the pigment within the cell indicates that the spectral assay is not influenced by the observed compartmentalization of the chromoprotein. However, the observed compartmentalization of phytochrome is correlated with a loss in spectrophotometrically detectable Pr.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00387878
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  • 3
    Electronic Resource
    Electronic Resource
    Autoradiographic studies of 3H-dexamethasone uptake by immunocytochemically characterized cells of the rat pituitary (1977)
    Springer
    Cell & tissue research 182 (1977), S. 347-356 
    Details
    ISSN: 1432-0878
    Keywords: Pituitary ; Dexamethasone ; ACTH ; Autoradiography ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary 3H-Dexamethasone (10 μg/kg) was injected intravenously in adrenalectomized rats and after survival times of 5, 30, 60, and 180 min its uptake within the pituitary was studied by autoradiography. Radioactivity was concentrated in cell nuclei in the pars nervosa and pars distalis. Within the pars intermedia, only cells of the marginal zone were labeled. In the pars distalis, some cells showed a weak nuclear accumulation of radioactivity as early as 5 min after injection. The tissue radioactivity was nearly maximal at 5 min, and the proportion of radioactivity in nuclei reached a maximum of 60–70% by 30 min. In competition experiments, non-radioactive steroids (1 mg/kg) were injected 5 min before 3H-dexamethasone and sacrifice was 30 min later. Dexamethasone markedly diminished the nuclear accumulation in the pars distalis, but corticosterone and progesterone did not. In the pars nervosa, corticosterone and progesterone competed for nuclear uptake of 3H-dexamethasone, although less effectively than dexamethasone itself. Different cell types in the pars distalis were characterized by treating autoradiograms with an immuno-peroxidase bridge procedure. Cells treated with anti-ACTH 17–39 had the greatest nuclear concentration of radioactivity, and those stained with anti-TSH were least heavily labeled. Cells treated with antisera to GH, PRL, and hCG were moderately labeled.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00219770
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  • 4
    Electronic Resource
    Electronic Resource
    Isolation, characterization and localization of bovine adrenal medullary myosin (1977)
    Springer
    Cell & tissue research 178 (1977), S. 17-38 
    Details
    ISSN: 1432-0878
    Keywords: Myosin ; Immunocytochemistry ; Adrenal medulla ; Exocytosis ; Secretion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Myosin was isolated in high purity from the bovine adrenal medulla by gel filtration and ion exchange chromatography. The purified myosin was analyzed by electrophoresis in gels containing SDS and found to contain a 200,000 molecular weight heavy chain and major light chains of molecular weights 20,000 and 17,000 in a 1∶1∶1 molar ratio. At high ionic strength the myosin had high Ca-ATPase and K-EDTA-ATPase activities and low Mg-ATPase activity. At low ionic strength, the Mg-ATPase was activated to a low level by rabbit muscle actin. The myosin was found to decorate F-actin in the absence, but not the presence of ATP. In low ionic strength solutions, the myosin assembled into characteristic bipolar filaments. The distribution of this myosin in the adrenal medulla and of cross-reacting myosin in several other bovine tissues was determined with the use of antimedullary myosin immunoglobulin G as a specific stain that was detected by direct and indirect immunofluorescence. In the medulla strong staining was seen between the chords of chromaffin cells indicating the presence of a highly muscular vasculature that may perform functions analogous to those of the myoepithelium of exocrine glands. The chromaffin cells showed weak positive staining around the nuclei and in a pattern radiating toward adjacent blood vessels. Cells of the inner zone of the adrenal cortex showed strong staining in the peripheral cytoplasm while cells in the intermediate and outer zones did not stain. In a blood smear, platelets and the cytoplasm of leukocytes stained strongly while erythrocytes did not stain. In striated muscle and the gray and white matter of the cerebrum only the capillaries and larger vessels stained. In the liver the phagocytic cells bordering vascular sinuses stained strongly while the hepatocytes were separated from one another by a 2 micron trilaminar band possibly representing the microfilament web surrounding the bile canaliculi and associated with junctional complexes. The results suggest that myosin is present in several highly differentiated, non-motile tissue cells where it may play a role in secretion or other specialized functions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00232821
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  • 5
    Electronic Resource
    Electronic Resource
    Anreicherung und Bestimmung von Blei in Boden- und Sedimentaufschlüssen und -extrakten mit der Flammenatomabsorption (1978)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 291 (1978), S. 213-216 
    Details
    ISSN: 1618-2650
    Keywords: Best. von Blei in Boden, Sedimenten ; Spektralphotometrie, Atomabsorption ; Flamme, Reextraktion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Zusammenfassung In Vorversuchen wurde gezeigt, da\ beim Aufbewahren der organischen APDC/MIBK-Extrakte starke Bleiverluste auftraten. Daher war es notwendig, das Blei mit SalpetersÄure in die wÄ\erige Phase zu reextrahieren. Wiederfindungsversuche bestÄtigten, da\ Extraktion und Reextraktion quantitativ verlaufen. Das Blei wird dabei um den Faktor fünf angereichert. Es werden mittlere Standardabweichungen für vier Konzentrationsbereiche angegeben. Als Nachweisgrenze wurde eine Konzentration von 0,16 mg Pb/l gefunden, die durch die Anreicherung auf ca. 0,03 mg Pb/l gesenkt wird. Arbeitsvorschriften für das Verfahren sind angegeben.
    Notes: Summary Preliminary investigations have shown, that considerable losses of lead occur during storage of the organic APDC/MIBK-extracts. Therefore, it was necessary to reextract lead into the aqueous phase by nitric acid. Investigations of recoveries confirmed, that extraction and reextraction of lead were quantitative. In this way the concentration of lead is increased by a factor of five. Mean standard deviations for four concentration ranges are given. A detection limit of 0.16 mg of Pb/l is obtained, which is lowered by the preceding enrichment to about 0.03 mg of Pb/l. Details of the procedure are given.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00480692
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  • 6
    Electronic Resource
    Electronic Resource
    Zur AAS-Bestimmung von Quecksilber in Zahnwurzeln und Kieferknochen (1978)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 293 (1978), S. 141-144 
    Details
    ISSN: 1618-2650
    Keywords: Best. von Quecksilber in Zähnen, Knochen ; Spektralphotometrie, Atomabsorption ; HNO3/HF-Aufschluß, Kaltdampfverfahren
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Zusammenfassung Es wird ein Verfahren zur Bestimmung von Quecksilber in Zahnwurzeln und Kieferknochen beschrieben. Die homogenisierten Proben werden in Teflonbomben unter Druck mit einem Gemisch von HNO3/HF aufgeschlossen. Die Quecksilberbestimmung erfolgt mit einem Atomabsorptionsspektrophotometer nach dem Kaltdampfverfahren. Unter Einsatz von 200 mg Probe können Quecksilbergehalte bis zu 0,1 μg/g bestimmt werden. Die relativen Standardabweichungen liegen im Arbeitsbereich von 10–100 ng Quecksilber zwischen 2,7 und 5,6 %. Es wurden Quecksilbergehalte von 〈0,1–187 μg/g in den Proben gefunden.
    Notes: Summary The homogenized samples are decomposed with a mixture of HNO3/HF in teflon vessels under pressure. The determination of mercury is carried out with an atomic absorption spectrophotometer by means of the cold-vapour technique. The limit of detection is 0.1 μg/g for a 200 mg sample. A relative standard deviation of 2.7–5.6% is achieved in the working range of 10–100 ng mercury. Mercury contents of 〈0.1–187 μg/g were found in the samples.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00486994
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  • 7
    Electronic Resource
    Electronic Resource
    Extraction and atomic-absorption spectrometric determination of traces of bismuth with zinc dibenzyldithiocarbamate in aluminium alloys and solder alloys (1978)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 292 (1978), S. 282-284 
    Details
    ISSN: 1618-2650
    Keywords: Best. von Wismut in Aluminiumlegierungen, Lötlegierungen ; Spektralphotometrie, Atomabsorption ; Zinkdibenzyldithiocarbamat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Summary A sensitive method for the extraction and atomic-absorption spectrometric determination of bismuth with zinc dibenzyldithiocarbamate (ZnDBC) has been developed for aluminium alloys and solder alloys. Bismuth is extracted from relatively strong acidic solution into methyl isobutyl ketone (MIBK) as BiDBC complex and its concentration is determined by spraying the MIBK extract into an air-acetylene flame. Iron and copper up to 1 mg resp. 100 μg did not interfere with the extraction of bismuth in the range of 0–80 μg. The limit of detection was about 0.12 ppm in MIBK.
    Notes: Zusammenfassung Ein empfindliches Verfahren zur Wismutbestimmung mit Zinkdibenzyldithiocarbamat wurde ausgearbeitet. Wismut wird dabei aus relativ stark saurer Lösung als Bi-DBC-Komplex mit Methylisobutylketon extrahiert und zur Messung der Extrakt direkt in die Luft-Acetylen-Flamme gesprüht. Bis zu 1 mg Fe und 100 μg Cu stören die Bestimmung von 0–80 μg Bi nicht. Die Nachweisgrenze beträgt 0,12 ppm in Methylisobutylketon.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00493696
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  • 8
    Electronic Resource
    Electronic Resource
    Comparative study of sodium borohydride tablet and tin(II) chloride reducing systems in the determination of mercury by atomic-absorption spectrophotometry (1978)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 292 (1978), S. 273-277 
    Details
    ISSN: 1618-2650
    Keywords: Best. von Quecksilber ; Spektralphotometrie, Atomabsorption ; Kaltdampf, Vergleich der Reduktion mit NaBH4-Tablette oder SnCl2-Lösung
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Summary An atomic-absorption spectrophotometric method for the rapid and precise determination of mercury has been studied by using the sodium borohydride tablet reduction and cold-vapour atomicabsorption procedure coupled with amalgamation on gold. The comparison of the sodium borohydride tablet and tin(II) chloride solution reducing systems are described in detail. The use of the sodium borohydride tablet wrapped in a wafer sheet is very simple and gives more precise results. From the comparison of the effect of diverse ions, it is obvious that both reducing systems have their merits and demerits.
    Notes: Zusammenfassung Ein atomabsorptions-spektralphotometrisches Kaltdampf-Verfahren zur schnellen Quecksilberbestimmung wird beschrieben, das auf der Reduktion mit Hilfe der Natriumborhydridtablette und der Amalgamierung mit Gold beruht. Der Vergleich mit der Zinn(II)-Chloridmethode wird diskutiert. Genaue Ergebnisse werden durch einfaches Einhüllen der Reduktionstablette in ein Waffelblatt erzielt, wodurch der Reaktionsbeginn verzögert wird. In bezug auf Störelemente ergeben sich für beide Verfahren Vor- und Nachteile.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00493694
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  • 9
    Electronic Resource
    Electronic Resource
    Bestimmung von Blei in kleinen Proben der marinen Diatomee Skeletonema costatum (Grev.) Cleve durch flammenlose Atom-Absorptions-Spektrometrie nach Flußsäureveraschung (1978)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 292 (1978), S. 278-281 
    Details
    ISSN: 1618-2650
    Keywords: Best. von Blei in Algen, Diatomeen ; Spektralphotometrie, Atomabsorption ; flammenlos, Störungen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Zusammenfassung Es wird eine nasse HF-HClO4Veraschungsmethode von biologischem Material in offenen PTFE-Reagensgläsern beschrieben. In anschließenden flammenlosen AAS-Messungen mit Hilfe der Deuterium-Untergrundkompensation und des automatischen Probenwechslers AS-1 konnte gezeigt werden, daß Silicium vollständig entfernt worden, kein meßbarer Matrix-Effekt festzustellen war und die Bleikonzentrationsbestimmung durch eine Eichgerade und durch Standard-Addition zu gleichen Werten führte. Die Methode ist mit einem Variations-Koeffizient von 0,45% behaftet.
    Notes: Summary A wet HF-HClO4 decomposition method for biological material in open PTFE-test-tubes is described. Flameless AAS measurements with the aid of deuterium background compensation using the auto sampling system AS-1 showed that Si was completely eliminated by the decomposition, that no measurable matrix effect could be detected, and that the values found for the lead concentration by calibration curve and standard addition were identical. The coefficient of variation for the method was V=0.45%.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00493695
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  • 10
    Electronic Resource
    Electronic Resource
    Determination of organosilicon compounds in water by atomic absorption spectroscopy (1978)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 292 (1978), S. 362-364 
    Details
    ISSN: 1618-2650
    Keywords: Best. von Siliciumorganoverbindungen in Wasser ; Spektralphotometrie, Atomabsorption ; Extraktionsverfahren
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Summary An extraction technique is described for the determination of ppm concentrations of both hydrophobic and hydrophilic methylsiloxane compounds in water, based on a single extraction with a mixed solvent of (1∶1) l-pentanol and methyl isobutyl ketone followed by the determination of the silicone compounds extracted into the mixed solvent by atomic absorption spectroscopy. The method is applicable over the concentration range 0.3–30 ppm Si. Standard deviation in the case of 10 ppm Si is 0.313.
    Notes: Zusammenfassung Zur Bestimmung von ppm-Konzentrationen an hydrophoben und hydrophilen Methylsiloxanverbindungen im Wasser wird ein Extraktionsverfahren beschrieben. Hierbei wird nach einfacher Extraktion mit l-Pentanol/Methylisobutylketon (1∶1) das Silicium im Extrakt mit Hilfe der AAS bestimmt. Der erfaßbare Konzentrationsbereich liegt bei 0,3–30 ppm Si. Die Standardabweichung beträgt bei 10 ppm Si 0,313.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00481552
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