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  • Articles  (12)
  • Autoradiography  (12)
  • Springer  (12)
  • Blackwell Publishers Ltd
  • Blackwell Publishing Ltd
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  • International Union of Crystallography
  • 2020-2022
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  • 1976  (12)
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  • Articles  (12)
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  • Springer  (12)
  • Blackwell Publishers Ltd
  • Blackwell Publishing Ltd
  • Elsevier
  • Emerald
    • +
Years
  • 2020-2022
  • 1995-1999
  • 1985-1989
  • 1975-1979  (12)
  • 1965-1969
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  • Biology  (12)
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  • Natural Sciences in General
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  • 1
    Electronic Resource
    Electronic Resource
    Autoradiography ofCoelopa frigida (Diptera) embryos, after labeling with3H-uridine during various phases of oogenesis (1976)
    Springer
    Development genes and evolution 180 (1976), S. 311-314 
    Details
    ISSN: 1432-041X
    Keywords: Autoradiography ; Nucleotides ; Embryogenesis ; Dipteran Oogenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The distribution of tritiated (3H) uridine or its derivatives in embryos of the kelp flyCoelopa frigida is analyzed by autoradiography. The radioactive label is introduced into the growing oocyte, after injectio into females at different time periods post eclosion. The majority of the label remains in the cytoplasm for the entire embryonic development and is presumably incorporated into stable RNA. Only when the precursor is injected toward the final stages of oogenesis, can presence of label be detected in the nuclei, for a short time period during the blastodern stage. Since no preferential location in nucleoli is found, it appears possible that labeled cytidine could have been derived from the original precursor which would be available for incorporation into DNA.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00848777
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  • 2
    Electronic Resource
    Electronic Resource
    Export of protein from the osteoclast as studied by electron microscopic autoradiography (1976)
    Springer
    Cell & tissue research 168 (1976), S. 89-99 
    Details
    ISSN: 1432-0878
    Keywords: Osteoclast ; Protein export ; Lysosome ; Electron microscopy ; Autoradiography
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The present electron microscopic autoradiographic study includes a quantitative analysis of osteoclasts in vitro using tritiated leucin as a protein tracer. A significant increase in the grain density over the ruffled border and the underlying resorption zone was demonstrated two hours post pulse whereas the grain density of the remaining cytoplasm was relatively constant. This indicates a transport of newly synthesized protein from the osteoclast to the extracellular resorption zone. Earlier histochemical and biochemical experiments suggest that the exported protein may represent lysosomal enzymes to be used in the extracellular bone degradation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00219726
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  • 3
    Electronic Resource
    Electronic Resource
    Quantitative autoradiographic assessment of 3H-estradiol uptake in immunocytochemically characterized pituitary cells (1976)
    Springer
    Cell & tissue research 166 (1976), S. 25-35 
    Details
    ISSN: 1432-0878
    Keywords: Pituitary gland ; Cell types ; Estrogen ; Autoradiography ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Dry-mount autoradiography was combined with peroxidase immunocytochemistry to examine estrogen uptake in four pituitary cell types. Quantification by silver grain counts was used to compare 3H-estradiol uptake in nuclei of pituitary cells 60 min after i.v. injection into short-term (control) and long-term ovariectomized and in long-term thyroidectomized rats. Under all three hormonal states, the order of labeling intensity was: gonadotropes 〉 somatotropes 〉 lactotropes 〉 thyrotropes. Long-term ovariectomy caused a significant increase in estrogen uptake of gonadotropes, somatotropes and lactotropes, while uptake in thyrotropes decreased. Long-term thyroidectomy decreased uptake in somatotropes, lactotropes and thyrotropes while gonadotropes remained unchanged.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00215122
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  • 4
    Electronic Resource
    Electronic Resource
    The saccus dorsalis of the rainbow trout, Salmo gairdneri Richardson (1976)
    Springer
    Cell & tissue research 167 (1976), S. 467-491 
    Details
    ISSN: 1432-0878
    Keywords: Saccus dorsalis, Salmo gairdneri Richardson ; Histology ; Ultrastructure ; Cytochemistry ; Enzyme-cytochemistry ; Autoradiography
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The saccus dorsalis of the brain of the rainbow trout, Salmo gairdneri Richardson, has been investigated by means of histological, cytochemical, enzyme-cytochemical, electron microscopical and autoradiographical techniques. The saccus dorsalis is a rostro-dorsal evagination of the diencephalic roof, and consists of a partly folded epithelial wall separating the cerebrospinal fluid from the meningeal matrix fluid. The well-developed vascular system around the epithelial wall, consisting of capillaries with different diameters, seems to be part of the pineal vascular system. No structures were found that may be involved in a possible mechanical or nervous blood flow control. The single-layered epithelium consists of highly specialized cells of one specific type. These cells are mainly characterized by infolded basal membranes, long microvilli of a peculiar shape, non-folded lateral membranes bordering intercellular spaces, apical concentrations of elongate and cupshaped macromitochondria, a basally located rough endoplasmic reticulum, an apically situated smooth endoplasmic reticulum and apical concentrations of micropinocytotic vesicles. Morphological evidence is presented of a multiple function of these cells: (1) fluid secretion, (2) extrusion of low molecular weight organic substances into the ventricular system, (3) uptake of high molecular weight substances, and (4) uptake of low molecular weight organic substances (aminergic neurotransmitters [GABA]) from the cerebrospinal fluid. The significance of light and dark cells is discussed. Indications of a possible innervation of the saccus dorsalis epithelial cells were not observed. The functional significance of the saccus dorsalis (possible analogue of the choroid plexus?) is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00215179
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  • 5
    Electronic Resource
    Electronic Resource
    Fluorescence histochemistry and autoradiography of adrenergic nerves in the renal juxtaglomerular complex of mammals and man, with special regard to the efferent arteriole (1976)
    Springer
    Cell & tissue research 169 (1976), S. 211-220 
    Details
    ISSN: 1432-0878
    Keywords: Kidney ; Fluorescence histochemistry ; Autoradiography ; Sympathetic nerves ; Efferent arteriole ; Juxtaglomerular apparatus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The adrenergic innervation of the juxtaglomerular complex was studied in kidneys from mice, rats, guinea-pigs, rabbits, cats, dogs, pigs, monkeys, and humans using fluorescence histochemistry of neuronal nor-adrenaline and autoradiography of 3H-noradrenaline. The localization of the nerves was established by phase contrast optics or by perfusing the vascular system with India ink. Adrenergic nerve terminals, exhibiting a formaldehyde-induced fluorescence and having the ability to take up and accumulate 3H-noradrenaline, were easily identified when they enclosed the glomerular afferent arteriole. They continued in between and close to the macula densa and lacis cells to supply the glomerular efferent arteriole. The nerves could be seen to accompany this arteriole for a considerable distance until they branched off to the vasa recta in the juxtamedullary region and to adjacent cortical veins. This innervation pattern was found to be a constant feature except in kidneys from guinea-pigs and cats, in which post-glomerular adrenergic nerves were not found in some of the superficial glomerular units. The fluorescence in all adrenergic fibres supplying the juxtaglomerular complex disappeared after removal of the aortico-renal ganglion, showing that they belong to a common system of renal sympathetic nerves.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00214209
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  • 6
    Electronic Resource
    Electronic Resource
    Specific binding of anti-myosin and -actin γ-globulins to the surface of trypsin-dissociated embryonic chick cells (1976)
    Springer
    Cell & tissue research 171 (1976), S. 351-358 
    Details
    ISSN: 1432-0878
    Keywords: Actin ; Myosin ; Plasma membrane ; Binding ; Antibodies 125I ; Autoradiography ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary 125I-labelled sheep anti-rabbit γ-globulin antibodies were used to locate rabbit antibodies to smooth- and striated-muscle actomyosins at the surface of trypsin-dissociated embryonic chick cells. Statistical analysis of electron microscope autoradiographs revealed that the plasma membrane of these cells was significantly labelled with both antibodies. Further tests revealed that there were a significantly greater number of antigenic sites present on the cell surface for the gizzard smooth-muscle antibodies than for those against pectoralis striated-muscle actomyosin. It was further shown that both the rate and extent of binding of the 125Ilabelled smooth-muscle actomyosin antibodies to the cells were greater than for anti-striated-muscle γ-globulins. Binding of the former was reduced to a level similar to that of 125I-NIS conjugate by preincubation of the y-globulins with smooth-muscle heavy meromyosin, while a similar reduction was observed when anti-pectoralis actomyosin was treated with actin. It was concluded that actin- and myosin-like proteins must now be considered as integral components of the plasma membrane. The authors wish to thank Dr. W. Sinclair (Zoology) and Miss S. Lutkins (Statistics Department) for assistance with the statistical analysis and are grateful to Professor N. A. Mitchison (Zoology Department, University College London) for providing a control sample of 125I-labelled sheep anti-rabbit γ-globulin, Dr. D. Catty (Experimental Pathology Department, Birmingham University) for donating sheep anti-rabbit serum and Dr. U. Gröschel-Stewart (Zoologisches Institut der TH., Darmstadt, Federal Republic of Germany) for the rabbit anti-actomyosin antibodies. Miss B. Morris and Messrs. P. C. Lloyd, D. Williams and J. Meredith gave skilled technical assistance
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00224659
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  • 7
    Electronic Resource
    Electronic Resource
    Investigations on the turnover of adrenocortical mitochondria (1976)
    Springer
    Cell & tissue research 172 (1976), S. 149-156 
    Details
    ISSN: 1432-0878
    Keywords: Adrenal cortex ; Mitochondria ; Ultrastructure ; Autoradiography
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The radioactivity decay of the mitochondrial compartment of 3H-thymidine-injected rats was followed by high resolution autoradiography. The number of days in which the radioactivity of the mitochondrial compartment is reduced to a half was calculated from the semilogarithmic plots of radioactivity versus time. Since DNA is a very stable molecule, it was assumed that this parameter can be an estimate of the half-life of adrenocortical mitochondria. It was found that the half-lives of mitochondria from the zonae glomerulosa, fasciculata and reticularis are, respectively, 8.20, 11.51 and 8.16 days.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00226023
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  • 8
    Electronic Resource
    Electronic Resource
    Autoradiographic study on the localization of estradiol-17β in neonatal mouse uterus and cervix (1976)
    Springer
    Cell & tissue research 174 (1976), S. 329-337 
    Details
    ISSN: 1432-0878
    Keywords: Estradiol ; Neonatal uterus ; Neonatal cervix ; Autoradiography
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The uptake of 3H-estradiol-17β in the neonatal mouse uterus and cervix has been studied by an autoradiographic method. When the radio-active hormone is administered in vivo and in vitro, grains are found to be concentrated above the nuclei both in the uterine and cervical epithelium and stroma. Grain counts revealed that the nuclear concentration of grains is higher at 4 h than at 2 h after isotope injection. The cervical epithelium has a higher nuclear concentration than the uterine epithelium both in vivo and in vitro. In the stroma, this situation is reversed except after in vitro treatment of the tissues. In the cervix, more of the hormone seems to be located within the nucleus while in the uterus a higher proportion of the grains are found in the vicinity of the nuclear periphery. Although the nuclear concentration of grains is higher at 4 h than at 2 h, the number of grains above the sections is lower at 4 h. Both in vivo and in vitro, the number of grains is higher above the stromal than above the epithelial compartments of the uterus and cervix. Five days old animals showed the same labeling pattern. The differences in uptake and distribution of 3H-estradiol are discussed in relation to other known differences in the hormone responsiveness in these tissues.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00220679
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  • 9
    Electronic Resource
    Electronic Resource
    Localization of K+-Stimulated p-NPPase in the lachrymal ‘Salt’ gland of Malaclemys, using cytochemical and autoradiographical techniques (1976)
    Springer
    Cell & tissue research 174 (1976), S. 417-426 
    Details
    ISSN: 1432-0878
    Keywords: Salt transport ; Reptilia ; Enzyme E.C. 3.6.1.3 ; Cytochemistry ; Autoradiography
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Using two independent techniques, histochemistry and autoradiography, an enzyme (E.C. 3.6.1.3.) has been localized on basolateral cell membranes of salt secreting cells in the lachrymal gland of Malaclemys. This enzyme is ouabain sensitive. In addition an L-tetramisole sensitive alkaline phosphatase is found in the same sites, and an ethacrynic acid sensitive K+-stimulated p-NPPase is found on the apical membrane. The significance of these results with regard to the location of the pump responsible for net transepithelial sodium transport is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00220684
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  • 10
    Electronic Resource
    Electronic Resource
    Non-diffusional distribution of radioactivity in the rat median eminence after intraventricular injection of 3H-LH-RH (1976)
    Springer
    Cell & tissue research 168 (1976), S. 411-418 
    Details
    ISSN: 1432-0878
    Keywords: Luteinizing hormone-releasing hormone ; Hypothalamus ; Rat ; Autoradiography
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The distribution of radioactivity in the rat hypothalamus after intraventricular injection of tritiated luteinizing hormone-releasing hormone (LH-RH) was studied by autoradiography and compared with that expected from Fick's second law which defines the character of ordinary diffusion. The results suggest that LH-RH penetrates through the ependyma and thin subependymal layer in the median eminence and into the suprachiasmatic area by ordinary diffusion. By contrast, concentration of radioactivity in outer layers of the median eminence is increased and, therefore, can not be explained by the diffusion law. In this region incorporation of radioactivity into cell bodies was demonstrated. In the subependymal layer of the median eminence a few rows of silver grains seemingly located in cellular processes were also observed. The diffusion coefficient in the periventricular zone was lower than that in the deeper tissue. It is suggested that the ependymal-subependymal complex of the third and lateral ventricles acts as a passive membrane towards LH-RH contained in the cerebrospinal fluid. The significance of this mechanism for the intrahypothalamic transport of RHs is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00215317
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