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  • Elasticity
  • Immunocytochemistry
  • Rheology
  • Springer  (5)
  • Air Force Geophysics Laboratory
  • Am. Geophys. Union
  • Blackwell Publishing Ltd
  • International Union of Crystallography
  • Springer Nature
  • 1985-1989
  • 1975-1979  (5)
  • 1960-1964
  • 1975  (5)
Collection
Publisher
  • Springer  (5)
  • Air Force Geophysics Laboratory
  • Am. Geophys. Union
  • Blackwell Publishing Ltd
  • International Union of Crystallography
  • +
Years
  • 1985-1989
  • 1975-1979  (5)
  • 1960-1964
Year
  • 1
    ISSN: 1432-0878
    Keywords: Growth hormone-release inhibiting hormone ; Somatostatin ; Hypothalamus ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Utilizing the unlabeled antibody enzyme method, we investigated the distribution of hypothalamic elements immunoreactive with antibodies to growth hormonerelease inhibiting hormone (GH-RIH). Immunostained elements, resembling neural processes, are distributed along a pathway corresponding to a portion of the tuberoinfundibular tract. However, GH-RIH fibers are caudal, dorsal and medial to LH-RH fibers detected by the same technique. Similar topographic arrangements are noted in coronal and sagittal sections. Comparable results were obtained with two different preparations of antisera to GH-RIH. No cell bodies specifically stained by anti-GH-RIH were detected. Our data agree with those of other investigators using immunohistochemical techniques.
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  • 2
    ISSN: 1432-0878
    Keywords: Neurophysin ; Vasopressin ; Median eminence ; Electron microscopy ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary With the use of tissue prepared by freeze-substitution and the unlabelled antibody enzyme technique, neurophysin and vasopressin were localized at the ultrastructural level in the posterior pituitary and median eminence of the guinea pig. In the posterior pituitary neurophysin was found in the large neurosecretory granules (1300–1500 Å) of axons, Herring bodies, and nerve terminals. In some of these axons immunoreactive neurophysin was found outside of granules in the axoplasm. By light microscopy neurophysin was found in both the zona interna and zona externa of the median eminence; this was confirmed by electron microscopy. In the zona interna as in the posterior pituitary, neurophysin was localized both inside and outside the large neurosecretory granules. In the zona externa, immunoreactive deposit was primarily located in granules with a diameter of 900–1100 Å in nerve terminals abutting on the primary portal plexus. The distribution of vasopressin paralleled that of neurophysin except that the hormone was rarely extragranular. These results demonstrate for the first time that both neurophysin and vasopressin are present in granules of axons that are in contact with the hypophysial portal vasculature.
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 164 (1975), S. 543-557 
    ISSN: 1432-0878
    Keywords: Magnocellular hypothalamic nuclei ; Fetal and neonatal pigs ; Anti-porcine neurophysin serum ; Immunocytochemistry ; Slab gradient polyacrylamide gel electrophoresis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The immunoperoxidase cytochemical reaction was applied to the localization of neurophysin-containing elements in the fetal and adult pig hypothalamus. In the 60 day fetal pig, cells of the supraoptic nucleus (SON) were the only structures in the hypothalamus in which neurophysin was detected. However, by 87 days the cell bodies in both the SON and paraventricular nucleus (PVN) contained neurophysin-like material. The distribution of immunoreactive material in the 111 day fetal animal was similar to that found in the adult pig. In transverse section of the mature pig the SON exists in two discrete components; an antero-lateral group of cells connected by scattered cells to a smaller postero-medial group. Anteriorly, the PVN appears as a line of cells bordering the third ventricle but as we proceed posteriorly the dorsal aspect expands laterally to give a wedge-shaped group of cells. In mid-sagittal sections, the cells of the PVN are distributed over a wide area of the anterior hypothalamus in a triangular profile. The borders between the SON and PVN became more difficult to define in medial sections than in lateral sections. Continuous gradient polyacrylamide gel electrophoresis was carried out on the neural lobe extracts from fetal, newborn and adult pigs. Proteins with an electrophoretic mobility similar to that of porcine neurophysins-I,-II and -III were present in the newborn and 98 day fetal pig. It is concluded that material immunoreactive with anti-neurophysin serum is present in the hypothalamus of the 60 day fetal pig. Furthermore, at late fetal development and during the postnatal period it is tentatively suggested that the neurophysin present in the pituitaries of these animals is chemically identical with that of adult neurophysin.
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  • 4
    ISSN: 1432-0878
    Keywords: Pituitary gland ; Cytology ; Pars tuberalis ; Immunocytochemistry ; Luteinizing hormone
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The objective was to acquire evidence regarding the secretory capacity of cells in the pars tuberalis of the rat pituitary by the application of immunocytochemical staining. For this purpose the conjugated antibody and immunoglobulin-enzyme bridge techniques were utilized with antisera to the following hormones of the pars distalis: human somatotropin, human thyrotropin, human β-melanotropin, ovine luteinizing hormone (LH), porcine β17–39-corticotropin, and β1–24-corticotropin. Only LH-containing cells were demonstrated. They were exceedingly rare in the cephalic pars tuberalis beneath the median eminence. The frequency of LH-cells was greater in the pars tuberalis associated with the infundibulum and increased distally. LH-cells were most common ventrolateral to the infundibular stem and occurred singly and in clusters. These results indicate that following hypophysectomy the portion of the pars tuberalis that remains in situ has the capacity to secrete only LH of all the pars distalis hormones.
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Medical & biological engineering & computing 13 (1975), S. 151-161 
    ISSN: 1741-0444
    Keywords: Elasticity ; Human left ventricle ; Modulus ; Stress/strain ; Thick-walled model
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Description / Table of Contents: Sommaire Le ventricule gauche (VG) est représenté par une enveloppe musculaire dont la performance est caractérisée par la pression de la chambre et la contrainte et la déformation sur la paroi ventriculaire; le module d'élasticité ventriculaire effectif du VG décrit la relation entre ces variables de la performance et représente donc la fonction du transfer du système physiologique du ventricule gauche. On présente une méthode de détermination indirecte du module effectifE du ventricule gauche. La méthode fait usage d'un modèle mathématique à paroi épaisse du VG et ayant un milieu isotropique homogène. On détermine des valeurs instantanées deE pour des sujets atteints de maladies cardiaques à étiologies variables, de façon à évaluer la réponse du VG aux surcharges chroniques de pression et de volume. Les valeurs résultantes deE sont utilisées diagnostiquement pour caractériser l'état physiologique du VG. Des valeurs normales de,E à la systole indiquent que la force de la contraction exercée par le VG est normale et qu'il s'est donc ajusté à la maladie cardiaque.
    Abstract: Zusammenfassung Das linke Ventrikel (LV) wird als Muskelschale dargestellt, dessen Leistung durch Kammerdruck und Beanspruchung der Ventrikulärwand beschrieben wird. Das effektive elastische Modul des LV bringt diese Leistungsvariablen aufeindander in Bezug und stellt daher die Ubertragungsfunktion des linken physiologischen Ventrikelsystems dar. Es wird ein Verfahren zur indirekten Bestimmung des effektiven Moduls (E) für das linke Ventrikel dargestellt. Das Verfahren verwendet das starkwandige mathematische Modell eines LV mit einem homogenen isotropen Medium. Sofortige E-Werte werden für Fälle mit Herzkrankheiten verschiedener Ursachen betimmt, um die Reaktion des LV auf chronische Überbelastung durch Druck und Volumen zu beaurteilen. Die sich für E ergebenden Werte werden diagnostisch dazu verwendent, den physiologischen Zustand des LV zu bestimmen. Normale Werte für E bei Systole bedeuten, daß die vom LV ausgeführte Kontraktion normal stark ist, was bedeutet, daß sich das LV auf die Herzkrankheit eingestellt hat.
    Notes: Abstract The left ventricle (l.v.) is represented as a shell of muscle whose performance is characterised in terms of the chamber pressure and stress/strain in the ventricular wall; the effective elastic modulus of the l.v. relates these peerformance variables, and hence represents the transfer function of the left ventricular physiological system. A method is presented for indirectly determining the effective modulusE for the left ventricle. The method employs a thick-walled mathematical model of the l.v. having a homogeneous isotropic medium. Instantaneous values ofE are determined for subjects with heart diseases of varied etiologies, in order to assess the responses of the l.v. to chronic overloads of pressure and volume. Resulting values forE are used diagnostically to characterise the physiological state of the l.v. Normal values ofE, at systole, indicate that the strength of contraction exercised by the l.v. is normal, and hence is an indication of the l.v. having adjusted to the heart disease.
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