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  • Scanning electron microscopy  (15)
  • Springer  (15)
  • Frontiers Media
  • 2015-2019
  • 1975-1979  (15)
  • 1975  (15)
Sammlung
Schlagwörter
Verlag/Herausgeber
  • Springer  (15)
  • Frontiers Media
Erscheinungszeitraum
  • 2015-2019
  • 1975-1979  (15)
Jahr
  • 1
    Digitale Medien
    Digitale Medien
    The surface of the pecten oculi in the pigeon (1975)
    Springer
    Cell & tissue research 157 (1975), S. 457-465 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Pecten oculi ; Pigeon ; Surface ; Function ; Scanning electron microscopy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary A scanning electron microscopic study of the pecten reveals the rib-like character of the pectinal folds, the nature of their connections with the base and bridge of the pecten as well as the presence of interconnections between the bundles of superficial collagenous fibres. It has been suggested that the pecten may have a mechanical significance, namely protection of the retina from the excessive movements of the vitreous humour. The relationship between the superficial membrane of the folds which is continuous, the intercellular spaces and the process of diffussion has also been discussed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00222599
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  • 2
    Digitale Medien
    Digitale Medien
    Osteoblasts and collagen orientation (1975)
    Springer
    Cell & tissue research 159 (1975), S. 73-80 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Osteoblasts ; Collagen orientation ; Parietal bone ; Rat, Rhesus monkey ; Scanning electron microscopy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Bone was removed from the calvaria of anaesthetized 70 g rats or freshly killed young monkeys and the fibrous periosteum dissected off the inner, formative surface under 0.15 M cacodylate buffer. The bone and undisturbed osteoblasts were fixed in 3% glutaraldehyde in the same buffer for 24 to 48 hours, critical point dried and coated with evaporated carbon and gold for scanning electron microscopy (SEM). Fields of osteoblasts were photographed and chosen cells dissected off the osteoid using a tungsten needle. The control of the dissection was made possible by the use of a system of real-time stereo TV-speed SEM. The fields were rephotographed and the orientations of the osteoblasts were compared with that of the underlying collagen fibres. 62% of all osteoblasts lay with their long axes within 15° of the collagen fibre orientation below and 80% within 30°. Montages of large areas of osteoblasts were also made, and then compared with ones of the same area after the cells had been stripped off on adhesive tape. In general, the orientation of the collagen tended to be the same as the cell that formed it. Collagen fibres below cells at the periphery of a domain sometimes had the orientation of the cells in the adjacent patch. It is not possible to determine whether the cells controlled the orientation of the collagen, or vice versa, from this experiment, but other SEM evidence suggests that the collagen orientation in hard tissue matrices depends on the freedom of cells to move with respect to the matrix surface.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00231996
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  • 3
    Digitale Medien
    Digitale Medien
    Scanning electron microscopic studies of the rat incisor odontoblastema (1975)
    Springer
    Cell & tissue research 159 (1975), S. 233-243 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Odontoblasts ; Predentine ; Dentine ; Calcification ; Scanning electron microscopy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary A scanning electron microscopic technique was used to investigate the surface structure of dentinogenically active odontoblasts. Thin pieces of rat incisors were fixed, rapidly frozen, freezedried at -70° C and fractured to expose new surfaces prior to examination in the SEM. Differences in the appearance of odontoblastic cell surfaces were seen, with the most extensive ridge formations at the distal part of the sides of the odontoblasts. The predentine area displayed a spongy structure which contrasted to the compact appearance of dentine. Results are discussed in relation to previous studies at the light microscopic and transmission electron microscopic levels.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00219159
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  • 4
    Digitale Medien
    Digitale Medien
    Electron microscopic studies of the lung of the frog (1975)
    Springer
    Cell & tissue research 160 (1975), S. 399-410 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Lung ; Frog ; Alveolar epithelium ; Mucus layer ; Scanning electron microscopy ; Transmission electron microscopy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Scanning and transmission electron microscopy were used to study the inner architecture of the frog lung. In some specimens the alveolar surface mucus layer was removed to permit the examination of underlying features. The inner surface of the frog's lung is covered by a layer of microvilli belonging to only one type of epithelial cells. The boundaries of these epithelial cells are demarcated by small ridges. Different degrees of lung expansion cause variations of the surface topography. The morphology of certain surface features is examined in detail. Several methods of drying the specimens are compared.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00222048
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  • 5
    Digitale Medien
    Digitale Medien
    Comparative scanning electron microscope study of boar, bull and ram spermatozoa (1975)
    Springer
    Cell & tissue research 161 (1975), S. 329-341 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Spermatozoa ; Boar, bull, ram ; Surface ultrastructure ; Scanning electron microscopy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Beschreibung / Inhaltsverzeichnis: Résumé La morphologie comparée des spermatozoïdes éjaculés de Verrat, Taureau et Bélier a été étudiée au microscope à balayage. Le sperme lavé est fixé dans le glutaraldéhyde ou le mélange acide picrique-formaldéhyde-glutaraldéhyde. Les échantillons sont le plus souvent désséchés par la méthode du point critique (Fréon) sur un filtre et aussi dans l'air sur une lamelle de verre. La tête des spermatozoïdes de ces trois espèces présente la même forme en pagaie aplatie formée de trois régions principales: les deux segments, antérieur, entouré d'un épaississement marginal, et équatorial de l'acrosome et la région postacrosomique. La plupart des differentiations de la lame postacrosomique décrites en microscopie électronique à transmission sont visibles à travers la membrane plasmique, particulièrement après dessication à l'air. La morphologie superficielle du cou et des différentes parties du flagelle est aussi observable. Des différences spécifiques sont mises en évidence: chez le verrat seulement, par exemple, la surface de l'acrosome apparaît granuleuse, et aucune bordure antérieure dentelée de la lame postacrosomique n'est visible. La microscopie à balayage permet d'observer les grands traits et de fins détails de la morphologie superficielle d'un échantillon de sperme et aussi d'étudier les effects de traitements sur des spermatozoïdes (congélation, extraction de l'acrosome).
    Notizen: Summary The comparative ultrastructure of ejaculated boar, bull and ram spermatozoa is studied by scanning electron microscopy. After washing, the spermatozoa are fixed in glutaraldehyde or in picric acid-formaldehyde-glutaraldehyde mixture. Samples are prepared either by critical point drying (Freon) on Millipore filters or by air drying on glass cover slips. In all the species studied, three regions may be distinguished in the paddle-shaped head of the sperm: an anterior segment (surrounded by the marginal thickening) and an equatorial segment constituting together the acrosome, and the postacrosomal region. Most of the feature of the postacrosomal lamina described in transmission electron microscopy are visible through the plasma membrane, particularly after air drying. The surface morphology of the neck and of the different segments of the flagellum is also evident. Some species differences are encountered, e.g. rough surface of acrosome and absence of serrations in postacrosomal lamina of boar spermatozoa only. The techniques employed result in good general morphology and fine resolution of surface detail of the sperm samples; they also permit analysis of spermatozoa treated by freezing or submitted to acrosomal extraction.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00220002
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  • 6
    Digitale Medien
    Digitale Medien
    The surface fine structure of the bovine subcommissural organ (1975)
    Springer
    Cell & tissue research 163 (1975), S. 125-132 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Bovine subcommissural organ ; Scanning electron microscopy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The bovine subcommissural organ was studied by using scanning electron microscopy. The most prominent finding was the existence of protruded and dilated endings of the ependymal cells. The majority of these cells were ciliated with two or more cilia; only a few unciliated cells were seen. Some pore-like structures were also seen on the surface. From the functional point of view, the most interesting finding was an amorphous heterogeneous material on the subcommissural ependyma. Especially in the caudal part of the organ this material accumulated in abundance. No real filamentous structures such as Reissner's fibre could be seen, however, it was assumed that the heterogeneous material corresponds to this formation. No supraependymal neurones were demonstrated.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00221721
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  • 7
    Digitale Medien
    Digitale Medien
    Neuronal migration during the early development of the cerebral cortex (1975)
    Springer
    Cell & tissue research 163 (1975), S. 313-325 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Neurogenesis ; Cerebral cortex ; Scanning electron microscopy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Fixed cerebral vesicles of mouse foetuses were fractured and examined with the scanning electron microscope. This method provides a study of the three dimensional developmental features of the pseudostratified columnar epithelium up to the formation of the early cortex plate. Matrix cells are a cell population of homogeneous shape, however, mitotic cells are easily identified by their spherical form. The external surface of the brain is formed by the closely packed end feet of these cells covered by a basal membrane. The formation of the cortical plate is the result of a continuous cell migration in columnar arrangement towards the pia. Glioependymal cells extend along the whole brain wall and most likely provide guidance for the migrating cell cords. The formation of the so-called migratory zone is a consequence of the growth of the basal and the horizontal prolongations of emigrating cells. The significance of the cell to cell contacts for the neuronal migration processes is discussed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00219466
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  • 8
    Digitale Medien
    Digitale Medien
    The distal ommatidium of the compound eye of the housefly (Musca domestica): A scanning electron microscope study (1975)
    Springer
    Cell & tissue research 159 (1975), S. 379-385 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Compound eye ; Musca domestica ; Ommatidium ; Distal retinula ; Scanning electron microscopy ; Corneal lens ; Corneal pigment cell ; Pseudocone ; Semper cell ; Basement membrane
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The distal aspect of the housefly ommatidium was surveyed by the scanning electron microscope. Attention was directed to the somal eminence of the superior central cell and the lens to large pigment cell junction. The underside of each lens facet exhibits six hexagonally arranged incisures. Into each of these indentations are fitted several large pigment cells. This hexagonal indentation appears to be a tenacious anchorage. Two corneal pigment cells laterally encircle the pseudocone and at their proximal extension they enclose the Semper cells and neck of the retinula. The somal eminence of the superior central cell is about 10 μm from the base of the corneal pigment cell enclosure. Micrographs were used to construct a diagram of the ommatidium above the basement membrane. Suggestions are made as to the functional correlates of the observed ommatidial structures.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00221784
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  • 9
    Digitale Medien
    Digitale Medien
    Light- and scanning electron microscopical studies of the hypothalamo-adenohypophysial portal vessels of the toad Bufo bufo (L.) (1975)
    Springer
    Cell & tissue research 162 (1975), S. 131-139 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Hypothalamo-adenohypophysial region Bufo bufo (L.) ; Portal vessels ; Methyl-methacrylate casts ; Light microscopy ; Scanning electron microscopy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The vascularization of the hypothalamo-adenohypophysial region of the toad Bufo bufo (L.) (Amphibia, Anura) was studied by means of light- and scanning electron microscopy. Special attention was given to the portal vascular system of the median eminence and the pars distalis. Course and arrangement of these vessels are described.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00223268
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  • 10
    Digitale Medien
    Digitale Medien
    Surface fine structure of the globiferous pedicellariae of the regular echinoid, Psammechinus miliaris gmelin (1975)
    Springer
    Cell & tissue research 162 (1975), S. 377-385 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Globiferous pedicellariae ; Psammechinus miliaris ; Microvilli ; Chemoreceptor ; Scanning electron microscopy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The globiferous pedicellariae of Psammechinus miliaris are described. Two fixation methods giving minimal distortion and rapid tissue hardening were adapted for soft tissue preparation for scanning electron microscopy. The pedicellarial valves are covered by a microvillous epithelium. The outer valve epithelial microvilli overlying red spherulocytes in the epidermis are characterized by a filament matrix radiating out from each microvillus. These microvilli may function in epidermal absorption of organic solutes. The inner valve microvilli are more densely packed and the filament matrix is absent. Ciliation is confined to the inner valve surface where the cilia are concentrated to form a distal sensory pad and sensory hillock. Behavioural evidence suggests a chemo- and mechanosensory role for the inner valve surface.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00220184
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  • 11
    Digitale Medien
    Digitale Medien
    A scanning electron microscopic study of the rat liver sinusoid (1975)
    Springer
    Cell & tissue research 164 (1975), S. 371-385 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Kupffer cell ; Endothelial cell ; Sinusoid ; Liver ; Scanning electron microscopy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The surface ultrastructure of Kupffer cells in the rat liver has been studied by scanning electron microscopy (SEM). The results demonstrate that Kupffer cells are both significantly different and clearly distinct from endothelial cells. Kupffer cells have neither pores (and/or “sieve plates”) nor fenestrations, all of which are present in endothelial cells. They possess a stellate shape, and only indirectly, with slender and irregular evaginations, contribute to the lining of the sinusoidal wall. Furthermore, the luminal surface in some areas contains a large population of short microvilli, microplicae and invaginations. These elements form a kind of microlabyrinth which may correspond to the “worm-like” structures described by transmission electron microscopy (TEM). In the present study, transition forms between endothelial and Kupffer cells were never found. On the contrary, considering the highly fenestrated nature of the endothelial cells, the Kupffer cells may, by ameboid movements, easily cross the overlapping barrier of the sinusoid and protrude into the lumen. Thus, acting as activated macrophages, the Kupffer cells might function to prevent the entrance of foreign material into the tissues of the liver through the fragile and highly fenestrated endothelium. Finally, the topographical reconstruction of the sinusoid by correlated SEM and TEM studies demonstrates that Kupffer cells, with their protruding cytoplasm and ability to extend into the lumen of the sinusoid, may actually change the caliber of the vessel, and thus function as a “sphincter” which causes a temporary arrest of the blood flow when the diameter of the sinusoidal lumen is reduced.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00223015
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  • 12
    Digitale Medien
    Digitale Medien
    Scanning electron microscope observations on the muscle innervation of Oikopleura dioica fol (appendicularia, tunicata) with notes on the arrangement of connective tissue fibres (1975)
    Springer
    Cell & tissue research 164 (1975), S. 357-369 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Neuromuscular junctions ; Appendicularia (Oikopleura dioica) ; Connective tissue fibrils ; Scanning electron microscopy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Critical point dried and fractured appendicularia of the species Oikopleura dioica have been examined in the scanning electron microscope. The dorsal nerve cord with ganglion cells and peripheral nerve fibres could easily be observed. Thick peripheral nerve fibres leave the nerve cord as bilateral pairs at constant intervals along the tail. Most of these fibres branch from the naked nerve cord, but some evidently originate in ganglion perikarya bulging out from the nerve cord itself. These paired peripheral nerves always have elaborate end-arborizations on the medial surface of the lateral muscle cells. They are accordingly interpreted as motor axons. Some thinner peripheral nerve fibres originate at irregular intervals from both the nerve cord and the ganglion cells. Due to the numerous extracellular fibrils that connect the bilateral layers of the epidermal fins and the muscle cells to each other, these thin nerve fibres can seldom be traced to their termination. A few ones can, however, be traced ventrally between the notochord and the muscle cells and seem to end in singular bulb-like expansions. Clusters of synaptic vesicles are present in transmission electron micrographs of such nerves, and they are accordingly believed to carry efferent impulses. The extracellular fibrils are arranged in a highly ordered pattern with thick bundles crossing the gap between the structures to be interconnected and with numerous radiating insertions on the surface of the tissues.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00223014
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  • 13
    Digitale Medien
    Digitale Medien
    The Surface structure of isolated pancreatic islet cells (1975)
    Springer
    Cell & tissue research 164 (1975), S. 467-471 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Pancreas ; Islets ; Scanning electron microscopy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The surfaces of isolated pancreatic islet cells were studied with the scanning and transmission electron microscopes. Islets were isolated from the pancreas of Wistar rats by collagenase treatment and were incubated either in glucose-free medium or in 300 mg% glucose for one hour. Immunoreactive insulin (IRI) in the media of both control and experimental preparations was assayed. Islets were then transferred to 4% glutaraldehyde, buffered with cacodylate, pH 7.4, and prepared for scanning and transmission electron microscopy. Cell masses average 200 μ in diameter. Alpha cells appear pyramidal in shape, are about 8 μ in diameter and appear in groups. Beta cells are round or oval in shape and have an average diameter of 10 μ. Glucose stimulation raised the IRI value tenfold and increased the number of blebs and other surface irregularities per unit area of beta cell surface. Comparison with transmission electron micrographs suggests that the blebs are related to the process of emiocytosis.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00219938
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  • 14
    Digitale Medien
    Digitale Medien
    A scanning electron microscopic study of rabbit spermatozoa in the female reproductive tract following coitus (1975)
    Springer
    Cell & tissue research 163 (1975), S. 29-44 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Spermatozoa ; Capacitation ; Uterus ; Acrosome Reaction ; Scanning electron microscopy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The surface morphology of rabbit spermatozoa, fixed in situ (female reproductive tract) and prepared for scanning electron microscopy by critical point drying, was studied for as many as 36 hours post coitum. The findings demonstrate that 1) spermatozoa in the reproductive tract following coitus exist as a heterogenous, morphological population and 2) with time, shifts within this population from one predominant morphology to another take place. In the fresh ejaculate, most spermatozoa have intact surfaces free of membranous disruptions. With time, a process of labilization (denudation) of the membranes covering the acrosomal region occurs in a progressively larger proportion of spermatozoa. The labilization originates by a process of vesiculation and/or vacuolation and leads to the appearance of a series of small fenestrations or perforations of the surface membranes. The perforations coalesce, and gradually larger areas of the surface membranes are eroded such that by 15 hours post coitum, the outer acrosomal membrane, as well as other acrosomal areas, are to varying degrees, directly exposed to the uterine milieu. Secretory granules, picked up by cilia and transferred to the spermatozoa become localized over the acrosomal region shortly after coitus. The possible significance of these time-dependent, morphological events with the phenomena of capacitation and the “true” and “false” acrosome reactions are discussed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00218589
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  • 15
    Digitale Medien
    Digitale Medien
    The reality of arthropod cuticular laminae (1975)
    Springer
    Cell & tissue research 163 (1975), S. 411-413 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Cuticle ; Laminae ; Arthropod ; Scanning electron microscopy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Examination of etched pyramids of decapod crustacean cuticles with the scanning electron microscope indicates that laminae are continuous around the angles of the pyramids. This observation is in direct contrast to the result expected on Bouligand's (1965, 1971) hypothesis and suggests that laminae may be real structures.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00219475
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