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  • Scanning electron microscopy  (13)
  • Springer  (13)
  • Nature Publishing Group
  • 1970-1974  (13)
  • 1974  (13)
Collection
Keywords
Publisher
  • Springer  (13)
  • Nature Publishing Group
Years
  • 1970-1974  (13)
Year
  • 1
    Electronic Resource
    Electronic Resource
    The development and fine structure of the ultimobranchial glands in larval Rana temporaria L. (1974)
    Springer
    Cell & tissue research 148 (1974), S. 69-82 
    Details
    ISSN: 1432-0878
    Keywords: Ultimobranchial glands ; Anuran larvae ; Metamorphosis ; Ultrastructure ; Scanning electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The fine structure of ultimobranchial (UB) gland cells from Rana temporaria larvae 48 h after hatching until the completion of metamorphosis is described. A single UB cell type is present, believed to be the characteristic C cell, in which secretory granules are first detectable in 8 day post-hatching larvae. These secretory granules show an intimate association with lipid droplets. Unusual membranous and crystalloid inclusions, which may represent yolk platelets, are found in UB glands of very small larvae. The significance of a range of UB organelles is discussed and some scanning electron micrographs presented. This report is believed to be the first published ultrastructural and scanning electron microscope study of larval anuran UB glands.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00224319
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  • 2
    Electronic Resource
    Electronic Resource
    Ultrastructural analysis of the human cerebral ventricular system (1974)
    Springer
    Cell & tissue research 150 (1974), S. 389-397 
    Details
    ISSN: 1432-0878
    Keywords: Choroid plexus ; Human fetus ; Modifications during development ; Scanning electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The ultrastructural organization of the human fetal choroid plexus was assessed with scanning electron microscopy. The membranous modifications of choroidal ependymal cells differ remarkably between 11 and 20 weeks of intrauterine development and suggest a variable functional capacity at different times of ontogenesis. Based upon existing data coupled with the ultra-architectural organization of cilia, clavate and linear microvilli are seen with scanning electron microscopy, a multiple functional role is hypothesized for choroidal ependymal cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00220145
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  • 3
    Electronic Resource
    Electronic Resource
    Taste bud types in fishes (1974)
    Springer
    Cell & tissue research 153 (1974), S. 151-165 
    Details
    ISSN: 1432-0878
    Keywords: Taste buds ; Fishes ; Chemoreceptors and Mechanoreceptors ; Scanning electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Within the epithelium of the head gut of the sword-tail (Xiphophorus helleri Heckel) there are three types of taste buds that can be identified with certainty in the scanning electron microscope. The breathing valves, which are situated behind the mouth region, bear relatively high epidermal papillae in which type I taste buds can be found. The middle region of the head gut is mostly occupied by low epidermal papillae containing type II taste buds. Type III taste buds are present within the metabranchial head gut; they never rise above the normal level of the epithelium. Taste buds of each type show two kinds of receptor villi within their receptor areas: tall villi (about 1,5×0,5 μm) and small villi (about 0.5×0.2 μm). A few tall villi belonging to solitary chemosensory cells are situated between the superficial epithelial cells of the tongue. In addition, the surface of the epidermal cells shows a delicate microridge system.—It is postulated that the taste buds of different types serve different functions: Taste buds within epidermal papillae may act both as chemoreceptors, and mechanoreceptors. Taste buds that never rise above the normal level of the epithelium perhaps act predominantly as chemoreceptors.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00226604
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  • 4
    Electronic Resource
    Electronic Resource
    The organization and gross mineralization patterns of the collagen fibres in sharpey fibre bone (1974)
    Springer
    Cell & tissue research 148 (1974), S. 83-96 
    Details
    ISSN: 1432-0878
    Keywords: Sharpey fibre bone ; Collagen orientation ; Mineralization ; Mammals ; Scanning electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Sharpey fibre bone specimens from cranial sutures and muscle attachment sites of rhesus monkeys and rats, and tooth sockets of several mammals (man, rhesus monkey and rat specimens mainly) were fixed, and either dried by critical point drying or freeze drying, or made anorganic with 1,2 ethane diamine or sodium hypochlorite, washed and airdried. The bone was examined by scanning electron microscopy. The packing density of the extrinsic fibres affected their cross sectional shape, the well separated fibres being the most uniform. Long parallel rows and groups of extrinsic fibres were observed. The fibre units that made up the Sharpey fibre bundles were sometimes distinct and mineralized as units. Mineralization was generally similar to the varied patterns encountered in root cementum. The diameters of the extrinsic fibres were similar at all sites and in all species; those in socket bone differed little from those in cementum. The intrinsic fibre orientation, although constant over fairly large areas, showed no consistent relation to the direction of entry of the extrinsic fibres. We would like to thank Mrs. E. Bailey and Mr. P. S. Reynolds for technical assistance.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00224320
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  • 5
    Electronic Resource
    Electronic Resource
    Scanning electron microscopy of the sensory surface of the tentacles of sea anemones and corals (1974)
    Springer
    Cell & tissue research 147 (1974), S. 149-156 
    Details
    ISSN: 1432-0878
    Keywords: Sensorysur face ; Tentacles ; Sea anemones ; Corals ; Scanning electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Examination of the sensory surface of the tentacles of sea anemones and corals prepared by the Freon critical-point method has revealed three primary structures: ciliary cones consisting of a cilium surrounded by a cluster of shorter stereocilia, single long cilia, and microvilli. The ciliary cones occur on, and the single cilia and microvilli occur both on and between, the nematocyst- and spirocyst-bearing regions of the tentacles. The similarity of the anthozoan ciliary cones to the ciliary sensory structures in the lateral line, and the auditory and vestibular organs of vertebrates is noted and the possible functional significance of the coelenterate structures is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00582789
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  • 6
    Electronic Resource
    Electronic Resource
    Morphological variations within a given area of articular surface of cartilage (1974)
    Springer
    Cell & tissue research 147 (1974), S. 163-167 
    Details
    ISSN: 1432-0878
    Keywords: Articular cartilage ; Scanning electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Samples of articular cartilage from four different human joints were obtained at surgery. Serial scanning electron micrographs taken at a magnification of 1000× were used to reconstruct an 0.25 mm2 area of articular surface. Within these given areas both normal and degenerated portions were seen. This study supports the concept that the surface morphology of articular cartilage varies from joint to joint and from area to area within a joint. This information should be useful for the interpretation of light and electron micrographs, as well as histochemical and biochemical data.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00582791
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  • 7
    Electronic Resource
    Electronic Resource
    Morphological changes in ciliary cells due to Heat exposure a scanning electron microscopic study (1974)
    Springer
    Cell & tissue research 148 (1974), S. 45-56 
    Details
    ISSN: 1432-0878
    Keywords: Ciliated cells ; Mucous membranes ; Trachea ; Heat exposure ; Scanning electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The mucociliary system of the respiratory tract is easily influenced by temperature — and humidity variations in the surrounding air. A series of functional in vitro experiments on heat-exposed rabbit trachea has been performed. The present investigation deals with the corresponding morphological changes of tracheal surface structures after exposure to temperatures between 40°C and 50°C and at a constant humidity of approximately 90 per cent. The following pathological findings were observed at a temperature of 42°C: 1. Fusion of cilia in their distal ends after 20–45 min., 2. appearance of submembranous vesicles without visible matrix after 45–165 min., 3. disappearance of vesicles and a definite deterioration of ciliary structures after 165 min.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00224317
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  • 8
    Electronic Resource
    Electronic Resource
    Experimental and structural analysis of the function of saccus vasculosus in rainbow trout, Salmo gairdneri (Richardson) (1974)
    Springer
    Cell & tissue research 148 (1974), S. 27-44 
    Details
    ISSN: 1432-0878
    Keywords: Saccus vasculosus ; Rainbow trout ; Osmoregulation ; Infundibular recess ; Scanning electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Injection of a saccus vasculosus homogenate from sea water adapted rainbow trout into the third brain ventricle of fresh-water rainbow trout increased the survival time of the latter in sea water of high salinity. This indicated an active role of saccus vasculosus in osmoregulation. Autoradiographic analysis of brains from injected fish that had received a saccus vasculosus homogenate from sea water adapted specimens, in which the coronet cells of saccus vasculosus had previously been labelled with 3H-glucoseamine, revealed actual incorporation of labelled material into brain cells, especially in the hypothalamic region. The intracellular distribution of labelled material in the coronet cells of rainbow trout labelled with 3H-glucoseamine strongly indicates that such material represents a secretory product; it is therefore probable that the observed incorporation of label into brain cells of injected fish actually reflects normal processing of material secreted from the coronet cells. In the scanning electron microscope, the inside wall of the third brain ventricle of the rainbow trout was in many areas found to be covered with cilia. These apparently serve to keep liquor in circulation. The observation, both in injected and control animals, of free globules among these cilia supports the idea of an intra-ventricular transport of detached coronet cell material to the infundibular recess, as indicated by the autoradiographic analysis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00224316
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  • 9
    Electronic Resource
    Electronic Resource
    Structure of rat liver sinusoids and associated tissue spaces as revealed by scanning electron microscopy (1974)
    Springer
    Cell & tissue research 148 (1974), S. 111-125 
    Details
    ISSN: 1432-0878
    Keywords: Liver ; Sinusoids ; Kupffer cells ; Spaces of Disse ; Scanning electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The inner surface of sinusoids and adjacent hepatocytes have been examined by scanning electron microscopy. The endothelial cells lining the sinusoids show large numbers of fenestrations which vary greatly in size and arrangement. Some are very small (0.1 μm) and arranged in clusters; others that are much larger (∼1.0 μm) are subdivided by slender strands of cytoplasm. At sites where the larger fenestrae are present it is evident that the endothelial lining of the sinusoid is double. This may represent a kind of structural assurance against complete breakdown of what seems to be a very thin and fragile endothelial wall. Junctions between adjacent endothelial cells have not been found in these preparations. The open continuity of the sinusoid is occasionally interrupted by slender extensions of cells morphologically distinct from the thin fenestrated endothelial cells. These possess a characteristically textured surface and are thought to represent stellate Kupffer cells. The SEM images describe the subendothelial Spaces of Disse as being larger and as having more extensive ramifications than is generally evident from transmission micrographs. The space, limited on one side by the hepatocyte with numerous microvilli and on the other by endothelial cells, appears actually to be only part of an extensive labyrinth of intercellular channels. These connect the more discrete Spaces of Disse and extend into the narrower spaces between the hepatocytes. The total effect of this system is to expose the greater part of the liver cell surface to the blood filtrate. Microvilli populate the hepatocyte surfaces except for narrow margins which border the bile canaliculi. Whether their presence coincides with the adsorbing surfaces and their absence with secreting surfaces can be decided best by experimental studies.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00224322
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  • 10
    Electronic Resource
    Electronic Resource
    Ovulation and the mechanism of follicle rupture (1974)
    Springer
    Cell & tissue research 149 (1974), S. 301-312 
    Details
    ISSN: 1432-0878
    Keywords: Graafian follicle ; Ovulation ; Cytoplasmic granules ; Papilloma ; Scanning electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Changes of rabbit germinal epithelium at different times after an ovulatory dose of human chorionic gonadotrophin (HCG) were studied by scanning electron microscopy. Surface cells far from follicles did not show any consistent changes after HCG. However, germinal cells on growing follicles increased in size, while their microvilli successively decreased in length and number and eventually disappeared. At six hours after HCG, the cells over the apex showed protruding blebs and vesicles. Pour hours later the surface cells at the base had increased from about 7 to 12 μ in diameter and the contours of intracellular round structures were just visible. Half-way up to the apex the surface cells were still larger and contained several prominent intracellular round structures. At the apex the cells were collapsed and occasionally missing just before rupture. As regards the interpretation of these findings and their relation to the mechanism of ovulation, much remains to be done. However, since distinct and consistent changes characteristic of the later stages before rupture were found, the scanning electron microscope appears to be a valuable and convenient tool to evaluate the effect of different substances or procedures on the ovulatory process.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00226765
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