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  • Light and electron microscopy  (15)
  • Immunocytochemistry
  • Springer  (16)
  • American Association for the Advancement of Science
  • 1990-1994
  • 1970-1974  (16)
  • 1950-1954
  • 1974  (16)
Collection
Keywords
Publisher
  • Springer  (16)
  • American Association for the Advancement of Science
Years
  • 1990-1994
  • 1970-1974  (16)
  • 1950-1954
Year
  • 1
    Electronic Resource
    Electronic Resource
    Anatomy of the giant dopamine-containing neurone in the left pedal ganglion of Planorbis corneus (1974)
    Springer
    Cell & tissue research 151 (1974), S. 369-384 
    Details
    ISSN: 1432-0878
    Keywords: Giant dopamine neurone ; Planorbis corneus ; Localization of dopamine ; Axon tracing ; Light and electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary There is a giant dopamine-containing cell (GDC) in the left pedal ganglion of Planorbis corneus. Some presynaptic endings of the GDC are located within the visceral and left parietal ganglia, other endings are located peripherally. Dense-cored vesicles of 50–250 nm diameter were observed in the perikaryon and primary axon of the GDC. Electron microscope histochemistry suggests that these vesicles contain dopamine. Vesicles with a similar appearance are present in some axonal processes located in areas of the nervous system known to contain presynaptic endings of the GDC. This neurone offers unique advantages for studying the role of neuronal dopamine.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00224547
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  • 2
    Electronic Resource
    Electronic Resource
    Some observations on the ultrastructure of the central amygdaloid nucleus in the cat (1974)
    Springer
    Cell & tissue research 151 (1974), S. 489-498 
    Details
    ISSN: 1432-0878
    Keywords: Limbic system ; Cat ; Amygdaloid body ; Light and electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The present study was undertaken to re-examine the central nucleus of the cat in Nissl stained sections, to describe its fine structural characteristics, and to compare the lateral subdivision of this nucleus with the putamen. In Nissl preparations, it was observed that the lateral subdivision of the central nucleus contains a fairly homogeneous population of small cells while the putamen contains both small and large cells. It is the presence of a few large cells at the lateral periphery of the lateral part of the central nucleus that makes the boundary between the two nuclei indistinct. Examination of the fine structure of the medial and lateral subdivisions of the central nucleus revealed the presence of many boutons containing flattened vesicles and fewer with spheroid vesicles. In contrast, most of the boutons in the putamen have spheroid vesicles. It is concluded that the lateral subdivision of the central nucleus may be distinguished from the putamen, except at its most lateral border, by its homogeneous population of small cells and its many boutons containing flattened vesicles.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00222994
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  • 3
    Electronic Resource
    Electronic Resource
    Luteinizing hormone —Releasing hormone (LH-RH) pathway of the rat hypothalamus revealed by the unlabeled antibody peroxidase-antiperoxidase method (1974)
    Springer
    Cell & tissue research 153 (1974), S. 211-217 
    Details
    ISSN: 1432-0878
    Keywords: Luteinizing hormone-releasing hormone ; Hypothalamus ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Utilizing the unlabeled antibody enzyme method, we report the distribution of hypothalamic elements immunoreactive with antibodies to luteinizing hormone-releasing hormone (LH-RH) in the rat. Immunostained elements, resembling neural processes, were distributed along a pathway corresponding to the tuberoinfundibular tract which appeared to terminate near vascular elements in the external layer of the preand post-infundibular median eminence. No cell bodies stained specifically for LH-RH. Similar topographic arrangements were noted (in coronal and sagittal sections) in diestrous females, ovariectomized females and a hypophysectomized male. The same results were obtained with three different preparations of antisera to LH-RH. Our studies agree with those of other investigators using immunohistochemical techniques as well as with localization studies of LH-RH in the hypothalamus using bioassay and radioimmunoassay. Our results suggest that the unlabeled antibody enzyme technique will have unique value for identifying and tracing fiber systems related to specific functions within the hypothalamus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00226609
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  • 4
    Electronic Resource
    Electronic Resource
    Regional differences in the distribution of golgi cells in the cerebellar cortex of man and some other mammals (1974)
    Springer
    Cell & tissue research 153 (1974), S. 219-226 
    Details
    ISSN: 1432-0878
    Keywords: Cerebellar cortex ; Man and other mammals ; Golgi cells ; Regional differences ; Light and electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The number of Golgi cells per unit volume was determined in different regions of the cerebellar cortex of man and of ten other mammals. Despite the general belief in the uniform architecture of the cerebellar cortex, regional differences in the distribution of Golgi cells were found. In the inferior parts of the vermis, the number of Golgi cells per unit volume is twice that in the corresponding hemispheres. In addition, there are differences between the anterior and inferior parts of the vermis. These differences are a feature of the cytoarchitecture of the cerebellum in man and all the investigated mammals. The ratio of Purkinje cells to Golgi cells was also determined and found to differ in different species. In man, this ratio is 1∶1.5, while in the monkey and cat it is almost 1∶1.9 and in the rat 1∶3.3. These differences in the ratio of Purkinje cells to Golgi cells are discussed from the point of view of cerebellar evolution.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00226610
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  • 5
    Electronic Resource
    Electronic Resource
    The aesthetes of chitons (1974)
    Springer
    Cell & tissue research 153 (1974), S. 383-398 
    Details
    ISSN: 1432-0878
    Keywords: Aesthete Organs ; Lepidochitona cinereus ; Cell types ; Secretory cells ; Light and electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The structure of the aesthete organs of the chiton Lepidochitona cinereus (L.) is described by light and electron microscopy. The observations do not discount a sensory function for these organs, but possible sensory structures such as microvilli and cilia are poorly organised. Most of the aesthete is taken up with large cells actively forming secretory products and apparently passing them proximally in the aesthete canal towards the animal. Comparisons are drawn with the brachiopod caecae.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00229166
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  • 6
    Electronic Resource
    Electronic Resource
    Development of rat dorsal root ganglion neurones (1974)
    Springer
    Cell & tissue research 153 (1974), S. 399-413 
    Details
    ISSN: 1432-0878
    Keywords: Spinal ganglia (Rat) ; Cell division ; Autoradiography ; Neurone morphogenesis ; Light and electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Pulse labelling with tritiated thymidine was used to determine the time of the final division of the neuroblasts which subsequently form rat lumbar dorsal root ganglion neurones. The final division occurred during a 4 day period, the maximum frequency being on day 12 of gestation. Separation of the ganglion cells into large light neurones and small dark neurones showed that the large light neurones were formed earlier than the small dark neurones. In both cases the final divisions occurred over a period of 3–4 days, but the peak rate of formation of large neurones was on day 12, and that of the small neurones was on day 13. Low power electron micrographs were used to measure mean cell diameter throughout development from day 11 of gestation until a postnatal age of 225 days. A marked increase in cell diameter occurred on day 15–15.5, about 3 days after the final cell divisions of the majority of the cells. The rate of growth increased just before birth, but no increase in mean cell diameter was found between day 21 of gestation and the third day postnatal. The growth was again rapid after this period until a plateau in cell diameter was reached about 33 days after birth.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00229167
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  • 7
    Electronic Resource
    Electronic Resource
    Chicken pars distalis development (1974)
    Springer
    Cell & tissue research 155 (1974), S. 291-320 
    Details
    ISSN: 1432-0878
    Keywords: Adenohypophysis ; Chicken ; Developmental endocrinology ; Epithelial differentiation ; Light and electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Anlagen of the pars distalis (Rathke's pouch), adjacent ectoderm, endoderm, infundibulum and mesenchyme left in situ were put in 3% glutaraldehyde between 1–3 pm and fixed overnight. Epon sections of this material from six White Leghorn, Gallus gallus, embryos fixed at each stage, and of two control partes distales from laying hens of the same flock were examined. At stage 17 (∼12 hours after formation) Rathke's pouch cells were stratified, uninnervated, non-vascularized and stellate, with high nucleo-cytoplasmic ratios and few organelles. Except for lipid inclusions, pouch cells did not appear appreciably different by stage 27, either regionally within the pouch wall or from the adjacent epithelioid cells. Apparent major changes indicative of cytoplasmic maturation by stage 27 included: reduction in number of polysomes; appearance of single-membraned, dumb-bell shaped to rounded, dense granula which were usually in basal position but also in areas of the Golgi apparatus; greater prominence of rough endoplasmic reticulum with cisternae containing dense material, and of the Golgi apparatus, notably with variously dense and/or coated vesicles and material; reduction in lipid inclusions by stage 24. Non-terminating axons in the infundibulum were first seen at stage 27. We found no convincing evidence for any possible morphogenetic or other relationship between the pars distalis and surrounding tissues. “Melanophore-stimulating hormone-activity”, reported to appear on day 5 of incubation, “stored thyroid-stimulating hormone” or pars distalis hormone granula or activities could not be identified. Rathke's pouch and other epithelioid structures may have been secreting, taking up nutrients and/or differentiating, activities which could be important for yolk sac development, nutrition and/or cytodifferentiation, respectively.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00222808
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  • 8
    Electronic Resource
    Electronic Resource
    Development of secondary lamellae of the gills of the trout, Salmo gairdneri (Richardson) (1974)
    Springer
    Cell & tissue research 151 (1974), S. 509-523 
    Details
    ISSN: 1432-0878
    Keywords: Gill ; Trout ; Secondary lamellae ; Development ; Light and electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A light and electron microscope study of secondary lamellae of trout developing at 10° C is described. Collagen is secreted by mesenchyme cells in the connective tissue of the developing gill filament. This becomes enclosed in infoldings around the peripheries of mesenchyme cells. These cells become aligned in a single plane within folds of basement membrane and epithelium. The basement membrane of opposite sides of the fold becomes connected by a thick layer of collagen. Blood spaces form around the margin of the fold, connecting afferent and efferent filament blood vessels. Endothelial granules form in cells lining the outer border of the marginal channel when blood flow begins. Rows of pillar cells separate from the proximal layer of mesenchyme cells as the secondary lamellae develops further. New secondary lamellae are added at the filament tips. At 28 days, the basement membrane consists of 2 layers, a fine fibrous layer and 5–15 orthogonally arranged layers of collagen fibres. By 31 days, the collagen is arranged at random and the layer is thinner. A clear layer is also present by 67 days. A multilayered epithelium containing mucous and chloride cells is present at 28 days. By 102 days, the secondary lamellae are covered by 2 layers of epithelial cells only. Chloride cells are present in much greater numbers in developing gills than in the adult.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00222996
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  • 9
    Electronic Resource
    Electronic Resource
    Coronet cells in the neurohypophysis of Mugil capito cuvier and valenciennes (Teleostei) from a hypersaline lagoon (1974)
    Springer
    Cell & tissue research 155 (1974), S. 449-453 
    Details
    ISSN: 1432-0878
    Keywords: Coronet cells ; Teleostei ; Neurohypophysis ; Hypersalinity ; Light and electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In several adult specimens of Mugil capito caught in a hypersaline lagoon, an intra-neurohypophyseal channel system lined with coronet cells has been observed. Coronet cells are normally found only in the saccus vasculosus, and in modern Teleostei, this structure is always completely separated from the neurohypophysis. The penetration of coronet cells into the neural lobe, may be an adaptive phenomenon possibly connected with an osmo-protective role of these cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00227008
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  • 10
    Electronic Resource
    Electronic Resource
    Microtubules and movement in the archigregarine, Selenidium fallax (1974)
    Springer
    Cell & tissue research 148 (1974), S. 331-345 
    Details
    ISSN: 1432-0878
    Keywords: Microtubules ; Movement ; Selenidium ; Pellicle ; Light and electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The archigregarine, Selenidium, is parasitic in the digestive tracts of some polychaete worms. The trophozoites of Selenidium are worm-like in appearance, and are attached by one end to the intestinal epithelium of the host, while the remainder of the organism performs rhythmic bending movements, reminiscent of nematode worms. The trophozoites have a multilayered, longitudinally folded pellicle, and beneath this are longitudinally oriented microtubules, arranged in precise fashions. The arrangements of both the pellicle, and the microtubules change during the bending movements. Furthermore, if trophozoites are treated with the drug colchicine, the sub-pellicular microtubules are destroyed, the patterns of pellicular folding are altered, and the trophozoites cease to move. The contribution of the pellicle and the microtubules to the characteristic movements of the trophozoites is evaluated and discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00224261
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  • 11
    Electronic Resource
    Electronic Resource
    The fine structure of somatic muscles of tardigrada (1974)
    Springer
    Cell & tissue research 149 (1974), S. 81-89 
    Details
    ISSN: 1432-0878
    Keywords: Muscle cells ; Tardigrada ; Light and electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The somatic muscles of the tardigrades Macrobiotus hufelandi and Milnesium tardigradum consist of elongated cells which are structurally independent of each other and insert in the body wall. The contractile part of the muscle cells contains thick and thin myofilaments. The thin filaments run together in typical dense bodies. In relaxed muscles clear A-, I- and H-zones are formed, and the dense bodies are often accumulated in groups. In ribbon like I-zones they are sometimes arranged side by side in rows and resemble fragmented Z-rods. The sarcoplasmic reticulum is localized primarily in the small region between the contractile apparatus and sarcolemma, and is represented by a system of smooth, flat, reticulated cisternae. The contractile apparatus of the somatic muscles is compared to that of smooth and obliquely striated muscles of several invertebrates. On the basis of certain distinguishing features of the contractile part of somatic muscles of tardigrades, it is suggested that these muscles should be referred to as a type intermediate between smooth and obliquely striated muscles.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00209051
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  • 12
    Electronic Resource
    Electronic Resource
    The aquatic egg of Nymphula nymphaeata (Lepidoptera: Pyralidae) (1974)
    Springer
    Cell & tissue research 149 (1974), S. 473-479 
    Details
    ISSN: 1432-0878
    Keywords: Aquatic eggs ; Insects ; Envelopes ; Respiration ; Light and electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The aquatic egg of Nymphula has no trabecular layer and no aeropyles. Four tubular micropyles only traverse the anterior part of the chorion. Presumably, the egg contains a sufficient quantity of air to allow the development of the caterpillar when the egg is immersed in paraffin oil. Under the chorion, the vitelline membrane possesses crypts and furrows which seem filled by air. This air can enter through the permeable regions of the vitelline membrane. The mucus, between the chorion and the under surface of the leaf, may constitute an external air reserve dependent on photosynthesis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00223026
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  • 13
    Electronic Resource
    Electronic Resource
    The innervation of purkinje fibres in the atrium of the avion heart (1974)
    Springer
    Cell & tissue research 150 (1974), S. 57-66 
    Details
    ISSN: 1432-0878
    Keywords: Purkinje Fibres ; Avian heart ; Innervation ; Light and electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The innervation of the Purkinje fibres in the atrium of the heart of the adult fowl was investigated by light microscopy, using the Champy-Maillet OsO4-ZnI2 technique and the cholinesterase reaction and by electron microscopy. After impregnation of the tissue with OsO4-ZnI2, the dark-stained nerve fibres were clearly visible on the unstained Purkinje fibres. In the upper part of the posterior wall of the right atrium, the diffuse portion of the conducting system is especially richly innervated by varicose and smooth nerve fibres. Some of these fibres are cholinesterase-positive. The terminal axons run in the space between the Purkinje fibres and the fibrocytic envelope. They are either naked or accompanied by Schwann cell processes. In addition to varicosities containing granular vesicles, there are varicosities containing agranular vesicles with oval profiles. In the “en passant” synapses, the width of the synaptic cleft between a varicosity and the Purkinje cell is about 600 Å. The innervation of the Purkinje fibres appears more like the innervation of smooth muscle than that of striated skeletal muscle. The possible role of Purkinje fibres as mechanical receptors is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00220380
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  • 14
    Electronic Resource
    Electronic Resource
    Hypothalamic projections to the amygdala in the cat (1974)
    Springer
    Cell & tissue research 151 (1974), S. 499-508 
    Details
    ISSN: 1432-0878
    Keywords: Limbic system ; Cat ; Amygdaloid body ; Hypothalamus ; Stereotaxic lesions ; Light and electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In order to determine the origin of the hypothalamo-amygdaloid connections in the cat, small lesions were placed at various rostro-caudal levels of the hypothalamus. The animals were sacrificed after a period of 4, 8 or 11 days and the brains stained with the Nauta (1957), Fink and Heimer (1967) or Wiitanen (1969) method for the demonstration of degenerating axons and their terminals. It was observed that the anterior hypothalamic nucleus sends a small projection to the medial subdivision of the central nucleus and to the basal and lateral nuclei of the amygdala. The lateral preoptic area sends a larger projection to the anterior amygdaloid area, both subdivisions of the central and basal nuclei, and to the lateral and medial nuclei. No degeneration was observed in the amygdala following lesions in the medial preoptic area, the ventromedial nucleus or the lateral hypothalamic area caudal to the anterior hypothalamic area. In a series of animals with lateral preoptic lesions, the site of termination of degenerating boutons on neurons of the amygdaloid nuclei was determined and the course of the degenerative process followed over a period ranging from 2 to 15 days. Many of the boutons, especially in the earlier stages of degeneration, appeared to be of the B3 type, containing flattened vesicles and forming symmetrical synaptic contacts with dendrites or somata. With longer post-operative survival times, however, they became increasingly electron dense and shrunken, so that the bouton type could not be determined.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00222995
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  • 15
    Electronic Resource
    Electronic Resource
    Morphology of the gill epithelium of the Lungfish, Lepidosiren paradoxa (1974)
    Springer
    Cell & tissue research 153 (1974), S. 365-381 
    Details
    ISSN: 1432-0878
    Keywords: Gills ; Lepidosiren paradoxa ; Epithelia ; Transport adaptions ; Light and electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The gills of Lepidosiren are very much reduced, consisting of a few lobe-like filaments. The gill epithelium differs from most other fish in being 4–7 layers thick. Three varieties of cell are described which occur in the inner epithelial layers. It is suggested that these represent stages in the synthesis of a granular secretory product. The superficial cells show characteristics of all 3 cell varieties, the granules apparently being voided into the microvillar pits at the surface. Surface cells are joined by junctional complexes typical of fish epithelia. Epithelial cells are separated by interstitial channels which are bridged by long cytoplasmic processes united by prominent desmosomes. Elongate mucous cells occur in large numbers. The Golgi apparatus is exceptionally large and well defined. It is characterised by accumulation of secretory products at numerous points along the cisternae. Wandering cells containing large granules and 2 types of leucocytes are also present in the interstitial channels. It is suggested that the gill epithelium is involved in active transport; pinocytotic vesicles are present on both sides of the inner epithelial cell layer and the blind interstitial channels are characteristic of transporting epithelia in which steady osmotic gradients operate.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00229165
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  • 16
    Electronic Resource
    Electronic Resource
    Effects of preganglionic sympathectomy on secretory changes in parotid acinar cells of rats on eating (1974)
    Springer
    Cell & tissue research 154 (1974), S. 135-150 
    Details
    ISSN: 1432-0878
    Keywords: Parotid glands (Rat) ; Secretion ; Sympathetic ; Autonomic innervation ; Cervical sympathectomy ; Light and electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Rats starved for 40 hours showed similar appearances in both parotid glands despite the fact that cervical sympathectomy had been performed on one side 24 hours before the tissues were removed. The acini of both glands of each animal were loaded with secretory granules and amylase estimations on both sides were similar. Rats starved for 40 hours and then fed chow for 1 1/2–2 1/2 hours showed variable depletion of secretory granules from their parotid acinar cells and corresponding alterations of amylase concentration. There was marked asynchrony in both granule depletion and the phase of resynthesis of individual cells. Rats starved during 40 hours and having had unilateral cervical sympathectomy 24 hours prior to a terminal eating phase of 1 1/2–2 1/2 hours showed big differences between the parotid glands on the different sides of each animal. The sympathectomised gland showed little or no depletion of secretory granules whereas the normally innervated gland showed a variable loss of granules, which was always greater than on the denervated side. Amylase estimations were also lower on the normally innervated side. It is concluded that normal reflex secretion of acinar granules in parotid glands of rats on eating requires intact sympathetic nerve pathways.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00223160
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