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  • Fine structure  (10)
  • Springer  (10)
  • 2020-2024
  • 1990-1994
  • 1970-1974  (10)
  • 1973  (10)
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  • Springer  (10)
Years
  • 2020-2024
  • 1990-1994
  • 1970-1974  (10)
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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 138 (1973), S. 299-314 
    ISSN: 1432-0878
    Keywords: Adenohypophysis ; Chicken ; Cytogenesis ; Fine structure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In the chick embryo the first membrane-bound secretory granules occur in the cytoplasm of occasional cells in the cephalic lobe of pars distalis at the 7th day of incubation. On the 8th day most of the cells in both the cephalic and caudal lobes contain secretory granules that are variable in size, form and density. On the 9th day at least two types of glandular cells are distinguishable in the cephalic and in the caudal lobes; however, these cells are not comparable with those of the adult gland. Differentiation of acidophils and basophils occurs, apparently simultaneously, in 11-day embryos. The cells of the cephalic and caudal lobes are morphologically distinct from their first appearance. Thus it is concluded that these two lobes develop independently and differently from an early stage of ontogenesis. The secretory granules are formed in the Golgi area of the hypophysial cells after the 8th day of incubation. However, secretory material may be synthesized also by a process not involving the Golgi apparatus. Nerve fibers containing granules first appear in the superficial layer of the median eminence on the 8th embryonic day and by the 12th day three types of granules and two types of clear vesicles are identifiable.
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 136 (1973), S. 191-205 
    ISSN: 1432-0878
    Keywords: Thymus ; Fine structure ; Avian ; Gallus domesticus ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The ultrastructure of the normal thymus of the young chicken (Gallus domesticus) is described. Four main cell types, lymphoid cells, epithelial cells, macrophages and myoid cells, can be distinguished. The lymphocytes are more numerous in the cortex than in the medulla, and are quantitatively the most important component of the thymus. The epithelial cells vary greatly in morphology. Reticular epithelial cells, which have long cytoplasmic processes connected by desmosomes, and which appear to afford a supporting network for the free cells of the thymus, are present in the cortex and medulla. Undifferentiated epithelial cells are present in the medulla and cortico-medullary regions and have few intracytoplasmic fibrils or desmosomes. Cystic epithelial cells, showing intercellular and intracellular cyst formation are frequent in the medulla. Also present in the medulla are squamous epithelial cells which contain many intracytoplasmic fibrils and have numerous desmosomes, and which are involved in the formation of Hassall's corpuscles. Macrophages are present in moderate numbers in the cortex and medulla, and immature and fully developed myoid cells are common in the medulla. Other cell types present include granule-containing cells with desmosomes, large pale cells with few cytoplasmic organelles, mast cells, plasma cells, red blood corpuscles and cells of the granular leukocyte series.
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 136 (1973), S. 277-289 
    ISSN: 1432-0878
    Keywords: Protonephridial system ; Turbanella cornuta Remane (Gastrotricha) ; Cyrtocytes ; Fine structure ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Die Protonephridien des marinen Gastrotrichs Turbanella cornuta Remane werden elektronenmikroskopisch untersucht. Die Terminalapparate der Macrodasyoidea sind Cyrtocyten. Ein Reusenröhrchen besteht aus acht Längsstäben mit netzartigen Zwischenwänden aus dünnen Fibrillen. Im Lumen des Röhrchens schwingt eine Geißel. Die Wand eines Terminalbechers bildet ein zusätzliches Reusensystem, das mit Poren und Schlitzen versehen ist. Die drei bis vier Cyrtocyten eines Protonephridiums münden in eine Sammelzelle ein. Von dieser geht ein Exkretionsröhrchen mit Treibwimperflamme aus, welches von einer Ausleitungszelle gebildet wird. Die Cyrtocyten der beiden Gastrotrichenordnungen (Chaetonotoidea u. Macrodasyoidea) grenzen sich strukturell gegenüber bisher bekannten Formen dieses Zelltyps ab.
    Notes: Summary The protonephridial system of the marine gastrotrich Turbanella cornuta Remane was studied with the electron microscope. The terminal cells of the Macrodasyoidea are Cyrtocytes. Each tube for filtration consists of eight longitudinal rods with a net of fine fibrils between them; it contains a single whip. The wall of a terminal cup with its pores and slits is an additional system for filtration. Three or four filtration tubes are ending in a collecting cell. From this cell an excretory channel with one whip formed by an outlet cell arises. The filtration tubes of Macrodasyoidea are similar to those of Chaetonotoidea and differ from other forms of this cell-type.
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 138 (1973), S. 455-474 
    ISSN: 1432-0878
    Keywords: Adenohypophysis ; Bird ; Prolactin cell ; Reproduction ; Fine structure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The anterior pituitaries from a series of female White-crowned Sparrows,Zonotrichia leucophrys pugetensis, in the periods of oviposition, incubation, and brooding under natural conditions, have been investigated by electron microscopy. The prolactin cells occur in cephalic lobe and are characterized by large (ca. 300–600 mμ), polymorphic electron-dense secretory granules and an extremely well developed, lamellated endoplasmic reticulum. During incubation and brooding it is only these prolactin cells that are in an activated secretory phase, as indicated by increase in number and size, extremely well developed endoplasmic reticulum, decrease in number of mature secretory granules, and by active formation of granules in the enlarged Golgi apparatus. In the late stages of brooding, and post-breeding, the prolactin cells regress with involution of the endoplasmic reticulum and Golgi apparatus, reaccumulation of granules, and the appearance of lysosomes. The gonadotropes of both the cephalic and caudal lobes undergo progressive morphologic changes through the course of the breeding period. They are numerous and active in the ovulating bird. They undergo gradual regression during the periods of incubation and brooding to become typical “broody” cells.
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 136 (1973), S. 579-590 
    ISSN: 1432-0878
    Keywords: Caddisfly larvae ; Abdominal chloride epithelia ; Fine structure ; Histochemical chloride localization ; Electrolyte transport ; Osmoregulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The larvae of the caddisfly Anabolia nervosa Curt. (Limnephilidae) possess 10 fields on the dorsal and ventral sides of the 3rd to 7th abdominal segments, which were formerly regarded as specialized sites of respiration. The epithelial fine structure and histochemical localization of chloride unequivocally show that the main function of these sites is the transport of electrolytes. They probably participate in osmoregulation by the absorption of salt. Therefore, these specialized areas of the hypodermis are termed chloride epithelia.
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 137 (1973), S. 177-194 
    ISSN: 1432-0878
    Keywords: Thyroid gland (Salamander) ; Epithelial cells ; Fine structure ; Vacuolar bodies ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The thyroid gland of adult salamanders, Hynobius nebulosus, in the breeding season was studied by electron microscopy. The follicular cells are different in cell height and fine structures; the taller cells with many cell organelles and granules and the lower cells with a few cell organelles and granules are both present in the same follicle. In the cytoplasm, three types of membrane-bounded granules, namely, cytosomes, colloid droplets, and vacuolar bodies and circular membrane complexes occur. The vacuolar bodies are subdivided into two types; the ordinary type having loosely distributed particles and the specific type containing tubules and/or closely packed filaments, crystalloid structures, except for the particles. The chromophobe colloids within the Bensley-cells correspond to extremely large, ordinary type vacuolar bodies, while the Langendorff-colloid cells possess increased numbers of granular cisternae of endoplasmic reticulum and a ribosome-rich, dense cytoplasmic matrix but not extremely large colloid. The intracytoplasmic circular membrane complexes appear in the Golgi area of cytosome-rich cells. It is suggested that they originate from the Golgi apparatus which was activated to produce many cytosomes. Intranuclear inclusions consisting of microtubules and filaments and tight junctions between two adjacent lateral plasma membranes are occasionally encountered.
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 140 (1973), S. 389-399 
    ISSN: 1432-0878
    Keywords: Muscle tissue ; Tardigrada ; Fine structure ; Polarisation and Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Die Muskelzellen des Pharynx-Bulbus der Tardigraden Macrobiotus hufelandi und Milnesium tardigradum sind bis zu 15 μm lang und bilden zwischen Basalmembran und cuticularer Intima des Lumens ein einschichtiges Epithel. Die Grenzen zwischen den Nachbarzellen zeigen einen geschwungenen Verlauf. Das Sarcolemm stülpt sich tief zwischen die Myofribrillen ein und bildet ein ausgeprägtes E-System, mit dem das sarcoplasmatische Reticulum unter Bildung von Diaden und Triaden korrespondiert. Die Myofibrillen verlaufen radial. Die dünnen Filamente entspringen am inneren und äußeren Sarcolemm aus hemidesmosomenartigen Strukturen in Form dichter Bündel, die sich im mittleren Teil der Fibrille, der dicke und dünne Filamente enthält, erweitern. Maximal 11 dünne Filamente konnten um die nicht immer streng hexagonal angeordneten dicken Filamente herum gezählt werden. Wie polarisationsmikroskopisch bestätigt werden konnte, besitzt jede Myofibrille eine breite A-Zone in der Mitte und an ihren Enden je eine schmalere I-Zone. Eine H-Zone ist undeutlich. Jeder Myofibrille kann der funktionelle und morphologische Wert einer Sarcomere zugeschrieben werden. Die Bedeutung dieser Befunde für die Evolution der Tardigraden wird diskutiert.
    Notes: Summary The muscle cells of the pharyngeal bulb of tardigrades Macrobiotus hufelandi and Milnesium tardigradum are up to 15 μm long and form a single layer between the basal lamina and the cuticle-coated lumen. The boundaries between adjacent cells are sinuous. The sarcolemma invaginates between the myofibrils whereby a marked E-system is formed. The sarcoplasmic reticulum is associated to the latter, constituting diads and triads. The myofibrils run radially. Thin filaments originate from hemidesmosome-like structures at the inner and outer sarcolemma in dense bundles which widen in the middle part of the fibrils. Each fibril contains thick and thin filaments in this region. As many as 11 thin filaments could be counted around a thick filament. The latter are not always arranged hexagonally. As it is confirmed by polarisation microscopy each myofibril has a wide A-Zone in the middle which is flanked by shorter I-zones. An H-zone is marked but indistinctly. Each myofibril is interpreted to have the functional and morphological equivalent to one sarcomere. The relevance of these findings in the evolution of Tardigrada is discussed.
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 146 (1973), S. 297-307 
    ISSN: 1432-0878
    Keywords: Adrenal cortex ; Prostaglandins E1 and E2 ; Fine structure ; Light and electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Histological and fine structural studies of adrenal cortices were performed on male Sprague-Dawley rats which had been given intravenous injections of prostaglandin E1 or E2. It was found that there were increased numbers of intracellular lipid droplets, free ribonucleoprotein particles, cholesterol ester clefts and coated vesicles of both the small and large varieties. A reorganization of the internal mitochondrial membranes and the appearance of protrusions of parenchymal cytoplasm into the sinusoidal lumina accompanied by vasodilation were also observed. These alterations are not typical of those observed following exogenous ACTH administration and are therefore considered to be prostaglandin-induced.
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  • 9
    ISSN: 1432-0878
    Keywords: Branchial gland ; Cephalopoda ; Fine structure ; Hemocyanin ; Histochemistry ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Licht- und elektronenmikroskopische Untersuchungen an der Branchialdrüse verschiedener Cephalopoden (Sepia officinalis, Octopus vulgaris, Loligo vulgaris, Eledone moschata) zeigen, daß das Drüsengewebe des stark vaskularisierten Organs aus einem sekretorisch aktiven Zelltypus mit ausgeprägtem endoplasmatischen Retikulum besteht. In den Zisternen des endoplasmatischen Retikulums lassen sich granuläre und ringförmige Strukturen (Durchmesser: 45–65 Å und 170 Å) nachweisen, die in Größe und Gestalt mit den Hämocyanin-Einheiten in den Gefäßen und Lakunen übereinstimmen und durch randlichen Zerfall der Zellen in den Blutraum auszuwandern scheinen. Die histochemischen Untersuchungen ergeben eine positive Reaktion für Monoaminoxydase, aber vergleichsweise geringe Phosphatase- und Dehydrogenase-Aktivitäten. Diese Befunde sowie der histochemisch nachgewiesene hohe Kupfergehalt des Drüsengewebes sprechen für eine hämopoetische, d.h. hämocyaninbildende Funktion der Kiemendrüse.
    Notes: Summary Light and electron microscopic investigations on the branchial gland of different Cephalopoda (Sepia officinalis, Octopus vulgaris, Loligo vulgaris, Eledone moschata) show that the gland tissue, strongly vascularized, consists of an actively secreting cell type with a remarkable endoplasmatic reticulum. In the cisternae of the endoplasmatic reticulum granular and ring-shaped structures can be identified (diameter: 45–65 Å and 170 Å) which correspond in size and shape with the hemocyanin units in the vessels and lacunae and which seem to emigrate into the blood space caused by the peripheral decay of the cells. Histochemical investigations show a positive reaction for monoaminoxidase, but comparatively low phosphatase and dehydrogenase activities. These results, and also the high content of histochemically identified copper in the gland tissue, seem to indicate a hemopoetic, i.e. hemocyanin-forming function for the branchial gland.
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 141 (1973), S. 223-233 
    ISSN: 1432-0878
    Keywords: Laryngeal muscles ; Tree Frog, Hyla aborea arborea (L.) ; Fine structure ; Motor endplates ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Um Hinweise auf die Funktion des Kehlkopfes bei der Rufabgabe zu bekommen, führten wir eine vergleichende elektronenmikroskopische Untersuchung an den vier Kehlkopfmuskeln rufaktiver Laubfrösche durch. Zum weiteren Vergleich studierten wir auch einen Extremitätenmuskel. Die Kehlkopfmuskeln sind untereinander gleich gebaut, unterscheiden sich aber in mehrfacher Hinsicht vom Extremitätenmuskel: Ihre Myofibrillen sind nur ein Drittel so dick. Die Kehlkopfmuskeln enthalten sehr viele, auffallend große und dichte Mitochondrien; das sarcoplasmatische Retikulum ist mächtig entwickelt. Sie enthalten außerdem sehr viel Fett, das stets mit den Mitochondrien assoziiert ist. In beiden Muskeltypen kommt Glykogen vor; seine Menge ist in den Kehlkopfmuskeln jedoch größer als im Extremitätenmuskel. Die Zahl der motorischen Endplatten ist in den Kehlkopfmuskeln 25 mal höher als im M. plantaris longus. Die Zahl der Triaden pro Sarcomer und ihre Anordnung ist bei den beiden Muskeltypen gleich. Die Kehlkopfmuskeln winterstarrer Hyla-Männchen unterscheiden sich in ihrem Feinbau und Fettgehalt nicht von den Muskeln rufaktiver Tiere.
    Notes: Summary In order to obtain information on the function of the larynx during vocalization, a comparative electron microscopic study was made of the four laryngeal muscles of male tree frogs druing their vocalizing phase. A skeletal muscle (m. plantaris longus) was examined for comparison. The fine structure of the laryngeal muscles is uniform, but differs from skeletal muscles in several respects: the diameter of the myofibrils of the laryngeal muscles is only 1/3 that of the skeletal muscle. They contain many strikingly large and dense mitochondria. There is a strongly developed sarcoplasmic reticulum. Laryngeal muscles contain large quantities of fat which is always associated with mitochondria. Laryngeal muscles contain more glycogen than skeletal muscle. The number of motor endplates in laryngeal muscles is 25 times larger than in m. plantaris longus. The number of triads per sarcomere and their location is the same in both types of muscle. The laryngeal muscles of hibernating and active tree frogs do not show any difference with respect to fine structure and amount of fat.
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