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  • Artikel  (27)
  • Light microscopy  (17)
  • Autoradiography  (11)
  • Springer  (27)
  • Wiley
  • 2020-2020
  • 1970-1974  (27)
  • 1945-1949
  • 1973  (27)
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  • Artikel  (27)
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  • Springer  (27)
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  • 2020-2020
  • 1970-1974  (27)
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  • 1
    Digitale Medien
    Digitale Medien
    Springer
    Calcified tissue international 12 (1973), S. 169-173 
    ISSN: 1432-0827
    Schlagwort(e): Calcification ; Histochemistry ; Autoradiography ; Fetal ; Mandible
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin , Physik
    Beschreibung / Inhaltsverzeichnis: Résumé Des rats femelles reçoivent du45Ca ou du32P au 13ème et 18ème jour de la grossesse. Les coupes sériées des têtes de foetus sont étudiées par autoradiographie et par diverses méthodes histologiques pour déterminer la calcification. La détection la plus précoce de45Ca s'observe simultanément comme une réaction positive pour le calcium avec une des méthodes histologiques utilisées.32P est en évidence par les méthodes autoradiographiques un peu plus tard que le45Ca et sa présence coincide avec la réaction positive la plus précoce observée avec les autres méthodes histologiques utilisées. Les os de ces têtes foetales commencent à se calcifier selon un mode particulier commençant par la mandibule, puis l'os frontal et le maxillaire supérieur, suivis par les os nasaux, pariétaux et interpariétaux.
    Kurzfassung: Zusammenfassung Weibliche Ratten erhielten45Ca und32P zwischen dem 13.–18. Tag der Schwangerschaft. Zur Ermittlung der Verkalkung wurden Serienschnitte der Köpfe ihrer Feten mittels Autoradiographie sowie durch verschiedene histologische Methoden untersucht. Das erste Auftreten von45Ca war ebenfalls von einer positiven Calciumreaktion mittels einer der verwendeten histologischen Methoden begleitet.32P wurde in der Autoradiographie erst etwas später als45Ca festgestellt und dessen Nachweis deckte sich mit den ersten positiven Reaktonen aller anderen verwendeten histologischen Methoden. Die Knochen in diesen Fetusköpfen begannen in einer bestimmten Sequenz zu verkalken: zuerst der Unterkiefer, dann das Stirnbein und der Oberkiefer, dann das Nasenbein, die Parietal- und Interparietalknochen.
    Notizen: Abstract Female rats were given45Ca or32P from 13 to 18 days of pegnancy. Serial sections from the heads of their fetuses were studied by autoradiography, as well as by several histological methods for assessing calcification. The earliest detection of45calcium occured at the same time as a positive reaction for calcium with one of the histological methods used.32P was not detectable by autoradiographic methods until somewhat later than45Ca, and its presence coincided with the earliest positive reaction with all of the other histological methods employed. The bones in these fetal heads began to calcify in a partcular sequence, the mandible first, then the frontal bone and maxilla, followed by the nasal, parietal and interparietal bones.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Springer
    Calcified tissue international 12 (1973), S. 125-136 
    ISSN: 1432-0827
    Schlagwort(e): Mineralization ; Molt ; Isopod ; Chemistry ; Light microscopy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin , Physik
    Beschreibung / Inhaltsverzeichnis: Résumé Au cours de la phase inhabituelle de mue d'un isopode d'eau courante,Lirceuts brachyrus, la moitié postérieure de l'exosquelette est éliminée 24 heures avant la moitié antérieure. A ce stade, une reminéralisation se développe dans la partie postérieure alors que la partie antérieure est dans un stade de pré-mue. Le pourcentage de différence en calcium dans les deux moitiés à mi-mue et mue complète est respectivement de 22% (p〈0.01) et 33% (p〈0.01), indiquant une complexation du calcium pendant la mue. La rapidité de la reminéralisation est illustrée par le fait que le contenu minéral total double dans la partie postérieure entre la mi-mue et la mue totale et dans la partie antérieure entre la fin de la mue et un jour après. Le carbonate de calcium, sous forme de calcite, a pu être identifié par diffraction électronique de coupes fines des téguments.
    Kurzfassung: Zusammenfassung Während der ungewöhnlichen Häutungssequenz des Frischwasser-IsopodenLirceus brachyurus (Harger) wird die hintere Hälfte des äußeren Skeletts 24 Std vor der vorderen Hälfte abgestoßen. In der halbgehäuteten Phase erfolgt Remineralisation im hinteren Teil, während der vordere Teil in einem Vorhäutungszustand ist. Der prozentuale Unterschied des Calciums in den zwei Hälften bei Halb- und Vollhäutungszustand ist 22% (p〈0,01) bzw. 33% (p〈0,01), was andeutet, daß Calcium während der Häutung abgesondert wird. Die Geschwindigkeit der Remineralisation erhellt aus der Tatsache, daß sich der Gesamtmineralgehalt im hinteren Teil zwischen Halt- und Vollhäutung, in der vorderen Hälfte jedoch zwischen Endhäutung und einem Tag nach der Häutung verdoppelt. Calciumcarbonat in kristalliner Calcitform wurde mittels Elektronendiffraktion von dünnen Hautschnitten nachgewiesen.
    Notizen: Abstract During the unusual molt sequence of the fresh-water isopod,Lirceus brachyrus (Harger), the posterior half of the exoskeleton is shed 24 hours before the anterior half. At the half-molt stage, occurs in the posterior part while the anterior portion is in a pre-molt condition. The percentage difference in calcium in the two halves at half-molt and full-molt is22 (p〈0.01) and33 (p〈0.01) respectively, an indication that calcium is sequestered during The rapidity of remineralization is illustrated by the fact that the total mineral content doubles in the posterior part between half and full molt and in the anterior half between the end of molt and one day after ecdysis. Calcium carbonate in the calcite cystalline form was demonstrated by electron diffraction of thin sections of the integument.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 3
    Digitale Medien
    Digitale Medien
    Springer
    Cell & tissue research 139 (1973), S. 83-94 
    ISSN: 1432-0878
    Schlagwort(e): Subcommissural organ ; Reissner's fibre ; Rana esculenta ; Light/Darkness ; Autoradiography
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The influence of long daily photoperiods and of continuous darkness on the secretory activity of the subcommissural organ (SCO) and on the growth rate of Reissner's fibre (RF) in Rana esculenta was studied in winter. The amount of aldehyde-fuchsin (AF) positive material in the apical cell parts of the SCO is smaller in light-adapted than in darkness-adapted animals. Differences in incorporation rate of 35S-cysteine between the SCO of light-adapted and of darkness-adapted animals, however, are small. The conclusion must be that light/darkness conditions only slightly influence the secretory activity of the SCO cells of Rana esculenta. In agreement with this conclusion is the observation that RF grew only slightly faster in the light-adapted than in the darkness-adapted animals. Under the conditions used RF was completely renewed in about 3 to 4 days. Influences of temperature on the activity of the SCO have been observed.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 4
    Digitale Medien
    Digitale Medien
    Springer
    Cell & tissue research 139 (1973), S. 179-200 
    ISSN: 1432-0878
    Schlagwort(e): Retino-preoptic pathway ; Optic tracts ; Rana temporaria ; Light microscopy ; Autoradiography
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The optic tracts and centres of optic terminals of Rana temporaria have been investigated with silver impregnation techniques after unilaterally cutting the optic nerve and autoradiographically after injection of a mixture of tritiated amino acids into the vitreous body of the left eye. The observations on the course of the optic tracts and on the optic terminals in the thalamus and optic tectum are to a great extent in agreement with those of other authors. The probability of a retino-preoptic pathway is supported by the detection of fibres running in dorsal direction in front of the place where the optic nerve penetrates the brain. In horizontal sections these fibres can be seen deviating from the optic tract, covering a short distance in frontal direction and then turning upward under an angle of about 90 degrees. They disappear between the ventral aldehyde-fuchsin positive cells of the preoptic nucleus. The ventral and median parts of the ipsilateral preoptic nucleus contain fragments of degenerated fibres. Autoradiographic data are also in favour of the presence of an ipsilateral retino-preoptic tract. After carefully counting the number of grains over left and right preoptic nucleus, it appeared that over the ventral and median parts of the left preoptic nucleus more grains occur than over the identical areas of the right one.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 5
    Digitale Medien
    Digitale Medien
    Springer
    Cell & tissue research 136 (1973), S. 355-364 
    ISSN: 1432-0878
    Schlagwort(e): Hypothalamo-hypophysial system ; Rana temporaria ; Blinding ; Illumination ; Autoradiography
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The influence of a long daily photoperiod and continuous darkness on the hypothalamo-hypophysial system of blinded frogs was studied. In the preoptic nucleus no difference could be observed between the two groups of blinded frogs neither in the amount of incorporated 35S-cysteine nor in the amount of aldehydefuchsin positive material nor in the nuclear volume of the secretory cells. These results were compared with those of an experiment with intact frogs kept simultaneously under the same environmental conditions. It appeared that the activity of the dark-treated blinded group was enhanced compared with the activity of the dark-treated intact group while the light-treated blinded frogs showed a decrease in activity compared with the similarly treated intact animals. Likewise the influence of a long daily photoperiod on the hypothalamo-hypophysial system of unilaterally blinded frogs was studied. The activity of the preoptic nucleus was lower on the side where the optic nerve had been cut than on the intact side. In the dorsal part of the preoptic nucleus a difference between the two sides could not be observed. The significance of these results is discussed with regards to a possible connection between retina and preoptic nucleus.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 6
    Digitale Medien
    Digitale Medien
    Springer
    Cell & tissue research 139 (1973), S. 473-485 
    ISSN: 1432-0878
    Schlagwort(e): Pineal gland ; Rabbit ; Architecture ; Autonomic innervation ; Light microscopy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary A light microscopic investigation of the rabbit pineal gland with the aid of silver-stained sections gave the following results. In the gland a medulla and a cortex can be distinguished, the medulla containing so-called light and dark pinealocytes, the cortex only light ones. Autonomic nerve fibres reach the pineal organ by two routes: (1) via the perivascular spaces of pineal blood vessels and (2) via two distinct nerve bundles, the nervi conarii. Bilateral superior cervical ganglionectomy revealed that these pinealo-petal nerve fibres are mainly orthosympathetic postganglionic. Intramural pineal neurones with synaptic-like structures on their cell bodies and dendrites point to the presence of a parasympathetic innervation next to the orthosympathetic one. Direct afferent or efferent neural connections with the brain appeared to be absent.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 7
    ISSN: 1432-0878
    Schlagwort(e): Neurosecretion ; Annelida ; Sexuality and Development ; Autoradiography
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Beschreibung / Inhaltsverzeichnis: Zusammenfassung Während der sexuellen Entwicklung vonPlatynereis dumerilii steigt die Zahl der Neurone, die Merkmale einer sekretorischen Aktivität aufweisen, parallel zum Wachstum und zur Reifung der Keimzelle an. Die cytologische Differenzierung eines Neurons zur neurosekretorischen Zelle ist mit einer Steigerung der Synthese Cystin-haltigen Materials gegenüber konventionellen Neuronen gekoppelt. Neurosekretorische Zellen treten erstmals in Erscheinung, kurz bevor die Oocyten in die zweite Oogeniephase eintreten, während der die Vitellogenese abläuft. Aufgetragen gegen den Oocytendurchmesser ergibt die Zahl der Neurone, die sekretorisch aktiv werden und3H-Cystin inkorporieren, eine ansteigende Kurve. Die Kurve biegt auf ein Plateau, wenn die Oocyten eine kritische Größe erreichen. Diese Größe markiert jenes Entwicklungsstadium, von dem ab eine Enthirnung eine normale Reifung nicht mehr verhindert. Die Zahl synthetisch tätiger Neurone steight erneut an, wenn die Oocyten die kortikale Struktur des reifen Eies entwickeln. Das Maximum wird kurz vor der Metamorphose erreicht. Während der epitoken Umwandlung sinkt die synthetische Aktivität rasch ab, um im schwärmbereiten Tier gänzlich zum Stillstand zu kommen. In Tieren, die nach der kritischen Phase zur caudalen Regeneration veranlaßt werden, fällt die Zahl markierter neurosekretorischer Zellen auf ein Niveau ab, das für junge, „vorkritische“ Individuen charakteristisch ist. Entsprechend wird in jungen, vorkritischen Tieren, die erst wenige endokrine Neurone besitzen, die Differenzierung weiterer neurosekretorischer Zellen durch eine Regeneration nicht stimuliert. Der zeitliche Verlauf der synthetischen Aktivität im Zuge der Entwicklung und die durch eine Regeneration erzwungenen Veränderungen sind somit reziprok zur Aktivität des Gehirns in Bezug auf die Ausschüttung des Juvenilhormons. Die Hypothese wird zur Diskussion gestellt, die sinkende Produktion des Juvenilhormons werde von der steigenden Produktion eines antagonistischen neuroendokrinen Prinzips begleitet oder verursacht.
    Notizen: Summary During sexual development ofPlatynereis dumerilii, the number of neurones exhibiting secretory activity rises in parallel with the growth and maturation of the germ cells. The cytological differentiation of neuroglandular properties is accompanied with an increased uptake of tritiated cystine. The first neurones developing the characteristics of secretory cells and incorporating cystine appear just before the oocytes enter the second growth phase which corresponds with vitellogenesis. When plotted against the oocyte diameter the number of labelled neurosecretory cells yields an ascending line, which becomes a plateau once the oocytes approach a critical size. This critical size defines the developmental stage beyond which decerebration does not prevent normal maturation. The neuronal synthetic activity rises again when the oocytes differentiate the cortical structure of ripe eggs. The curve displays its maximum slope at the onset of metamorphosis. During the epitokous transformation the synthetic activity decreases rapidly and is almost totally depressed when swarming is imminent. In animals induced to regenerate their posterior segments after they have passed the critical stage, the number of labelled neurosecretory cells becomes reduced to the precritical level. According to this, regeneration in young precritical animals containing only few endocrine neurones does not stimulate the development of further secretory neurones. Thus, the time course during maturation and the changes caused by regeneration are reciprocal to the activity of the brain with respect to the release of juvenile hormone. The question is thus raised as to whether the declining titer of juvenile hormone may be accompanied or caused by an increased production of an antagonistic principle related to maturation.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 8
    Digitale Medien
    Digitale Medien
    Springer
    Cell & tissue research 138 (1973), S. 63-73 
    ISSN: 1432-0878
    Schlagwort(e): Adenohypophysis ; Rat ; Gonadotrophic cells ; Cycle, influence of testosterone ; Light microscopy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary 4-day cyclic adult female Wistar rats were injected subcutaneously with testosterone propionate on diestrus 1 at 16:00 and on diestrus 2 at 10:00 respectively. Non-injected females served as controls. Autopsy was performed on diestrus 2 at 23:00, and on proestrus at 14:00 and 17:00 respectively. The blue Alcian-PAS staining was used to evidence FSH (β) and LH (γ) pituitary cells. In control animals and in diestrus 2 injected females only a small number of FSH cells could be detected on diestrus 2 at 23:00. This number increased markedly on proestrus at 14:00 and decreased on proestrus at 17:00. A similar evolution was observed in diestrus 1 testosterone injected females, but the number of FSH cells appeared higher at any stage of autopsy in these females than in diestrus 2 injected females and in control rats. In control females, numerous LH cells were observed on diestrus 2 at 23:00. The number of these cells was diminished on proestrus at 14:00 and still more at 17:00. On the contrary few LH cells were detected in testosterone injected females on the evening of diestrus 2. An increase of these cells occurred on proestrus at 14:00, followed at 17:00 by only a weak diminution as established by comparison with control animals. An inhibition of FSH release and a suppression of the proestrus surge of LH were therefore supposed to cause, on one hand, the slowing up of follicular growth observed in diestrus 1 injected females and, on the other hand, the blockage of ovulation noted in both diestrus 1 and diestrus 2 treated animals.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 9
    ISSN: 1432-0878
    Schlagwort(e): Hippocampal region (rat) ; Distribution of dehydrogenases ; Postnatal development ; Histochemistry ; Light microscopy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Succinate-, α-glycerophosphate-, NADH-, and NADPH dehydrogenases (tetrazolium reductases) were studied histochemically in brain sections. The distribution of these enzymes in the hippocampal region was mapped in different postnatal stages. In addition to the hippocampus and the dentate area which have also been dealt with by other authors, the description includes the entorhinal area, parasubiculum, presubiculum, and subiculum. In all the areas of the hippocampal region the enzyme activity is first localized mainly in cell bodies. Activity in the neuropil shows a tendency to increase with age throughout the stages studied. Examples of particularly early or late differentiation of the histochemical pattern exist. Thus already after 4–6 days the differentiated staining pattern in the hippocampal layers as seen in the adult, begins to appear. In the entorhinal area the early enzyme distribution agrees with that in the adult and facilitates identification of its layers. After 22 days the fundamental enzyme chemoarchitectonics of the adult rat brain has been obtained. Histochemical characteristics of the different areas, support the view that the hippocampal region constitutes an entity.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 10
    Digitale Medien
    Digitale Medien
    Springer
    Cell & tissue research 141 (1973), S. 375-400 
    ISSN: 1432-0878
    Schlagwort(e): Hippocampal region (rat) ; AChE distribution ; Postnatal development ; Histochemistry ; Light microscopy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Acetylcholinesterase (AChE) in the hippocampal region was studied histochemically in different postnatal stages. Enzyme changes are described for the hippocampus proper, the dentate area, the entorhinal area, parasubiculum, area retrosplenialis e, presubiculum, and subiculum. In 1 day old animals there are only traces of AChE in solitary perikarya of the hippocampal stratum radiatum, while there is no activity in the neuropil. This is in contrast to other regions of the brain, e.g. the corpus striatum and the medial septum. Enzyme activity in the neuropil, although very weak, is seen clearly on the 2nd day. While the enzyme reaction in some cell soma seems to be permanent and also present in adult rats, other cell bodies, e.g. in the entorhinal area lose AChE activity during the last part of the 2nd week and in the 3rd week. Similar observations have been made on other parts of the central nervous system. The main features of chemodifferentiation in the hippocampal region seem to be completed by the end of the third week, although most layers and areas have not yet achieved the same high staining intensity as seen in the adult rats.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 11
    Digitale Medien
    Digitale Medien
    Springer
    Cell & tissue research 142 (1973), S. 131-146 
    ISSN: 1432-0878
    Schlagwort(e): Epidermis (mouse) ; Pattern of organization ; Proliferation kinetics ; Three-dimensional analysis ; Light microscopy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Beschreibung / Inhaltsverzeichnis: Zusammenfassung 1. Es wird eine Methode zur Herstellung, Montierung und Färbung von epidermalen Häutchenpräparaten zur dreidimensionalen Analyse des Epidermis-Aufbaues beschrieben. Eine Kombination mit der Histoautoradiographie ist bedingt möglich. Damit ist eine Basis für weitere Untersuchungen mit neuer Fragestellung geschaffen. 2. Die untersuchten Objekte Mäuseohr und Rückenhaut zeigen eine bisher unbekannte, auffallende Musterbildung mit Zuordnung bestimmter interfollikulärer basaler Gruppen zu Zellen des Stratum spinosum und des Stratum corneum. Es handelt sich um rosettenähnliche Gebilde aus mehreren peripheren Basalzellen und meist einer zentralen Spinosumzelle. 3. Die Bedeutung der Befunde für die normale wie für die pathologische Regeneration und Neoplasie der Epidermis ist offen. Es werden Beziehungen zum Regenerationsmodus und damit zur Proliferationskinetik der Epidermis diskutiert. In diesem Zusammenhang sind die Untersuchungen auch für die Humanpathologie relevant.
    Notizen: Summary 1. A method for the preparation, mounting, and staining of thin preparations of mouse epidermis is described, which allows a three-dimensional analysis of epidermal structure. A limited combination of this technique with histo-autoradiography is possible, thereby presenting a broader basis for further investigations. 2. Both ear and back skin show a striking, previously unrecognized pattern of organization, in which definite interfollicular basal cell groups are oriented towards cells of the stratum spinosum and stratum corneum. A rosettelike picture of many peripheral basal cells and, in most instances, a central spinosum cell is found. 3. The significance of our findings for normal and pathological regenerations as well as neoplasia of the epidermis remains open. We discuss relationships to the mode of regeneration and thus the kinetics of proliferation in the epidermis. Our investigations are also relevant for human pathology in this regard.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 12
    Digitale Medien
    Digitale Medien
    Springer
    Cell & tissue research 146 (1973), S. 491-500 
    ISSN: 1432-0878
    Schlagwort(e): Hypothalamo-hypophysial system ; Pineal complex ; Rana esculenta ; Light/darkness ; Autoradiography
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary InRana esculenta the activity of the hypothalamo-hypophysial system has been investigated in intact and blinded animals, after removal of the frontal organ and after elimination of the whole pineal complex. The results on intact and blinded animals are practically the same as those found earlier inRana temporaria. Light increases the activity of the preoptic nucleus, blinding changes the difference between light- and darkness-treated intact animals. Compared with the intact animals the other two groups (without frontal organ and without pineal complex) show a marked increase in incorporation of35S-cysteine in the preoptic nucleus. A difference between the animals without frontal organ and those without pineal complex, however, can hardly be observed. It follows that the absence of the frontal organ has the same influence on the preoptic nucleus as elimination of the whole pineal complex. In intact animals the influence of the frontal organ on the preoptic nucleus consists of an inhibition of neurosecretory activity as well under light as under darkness conditions. The enhanced synthesis of labelled material in the preoptic nucleus after removal of the frontal organ, results in a transport along the hypothalamo-hypophysial tract mainly to the outer zone of the median eminence and not to the posterior lobe.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 13
    Digitale Medien
    Digitale Medien
    Springer
    Cell & tissue research 144 (1973), S. 573-583 
    ISSN: 1432-0878
    Schlagwort(e): Gills ; Tilapia mossambica ; Blood vessels ; Arteriovenous anastomoses ; Light microscopy ; Quantitative evaluation
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The non-respiratory vascular system of T. mossambica gill filaments was studied in serial longitudinal and cross sections. Comparatively few scattered vascular communications occur between the afferent filament artery and the central venous sinus (AVAaff). The efferent filament artery, however, is connected by regularly arranged anastomoses (AVAeff), directly, and sometimes indirectly via nutritive vessels, to the central sinus. These AVAeff are about as numerous as lamellae counted on one side of each filament, although they diminish slightly in number towards the filament base. The relation AVAeff to AVAaff was 17.6:1 in the distal and 17.8:1 in the basal filamental region, while in the tip region of 7 filaments 126 AVAeff but only 1 AVAaff were encountered. No direct connection between the lamellar lacunae and the central sinus was detected. According to these results, non-respiratory intrafilamental blood shunting appears unlikely. AVAeff are assumed to be the main route for blood entering the central venous sinus which would consequently flow into the branchial veins.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 14
    Digitale Medien
    Digitale Medien
    Springer
    Cell & tissue research 139 (1973), S. 431-450 
    ISSN: 1432-0878
    Schlagwort(e): Cardiac muscle ; Frog ; Regeneration ; Electron microscopy ; Autoradiography
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary From the 5th day up to the end of 3rd week following local crushing of the frog ventricle myocardium, ca. 13% of myocyte nuclei, in the vicinity of the damaged zone, were labelled after a single 3H-thymidine (3HTdr) injection, and 30–50% of these were labelled after repeated 3HTdr administration. The number of myocyte mitoses was maximal (ca. 1.3%) at the beginning of the 3rd week. The reactive proliferation of myocytes was accompanied by their “partial dedifferentiation”. This involved the nuclear euchromatic rearrangement, increase in size of nuclei and nucleoli, accumulation of the sarcoplasm rich in free ribosomes and rough endoplasmic reticulum, hyperplasia of the Golgi apparatus, and the appearance of 80–100 Å in diameter cytofilaments. Electron microscope autoradiography has shown that all these changes may be more or less pronounced in myocytes incorporating 3HTdr. The myofibril ultrastructure was found to be unchanged during S phase. However, in the mitotically dividing myocytes, the majority of Z-disks were disintegrated resulting in progressive release of myofilament bundles. Both 3HTdr labelled and mitotic myocytes were anchored to the adjacent ones by desmosomes and intercalated disks. No free myoblasts were observed.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 15
    Digitale Medien
    Digitale Medien
    Springer
    Cell & tissue research 141 (1973), S. 493-503 
    ISSN: 1432-0878
    Schlagwort(e): Pituitary ; Glomerular body ; Marine teleost ; Hexagrammos otakii ; Larval osmoregulation ; Light microscopy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary In the larvae of the marine teleost, Hexagrammos otakii, the prolactin, ACTH, STH and pars-intermedia cells as well as the neurohypophysis and glomerular body showed no histological change at the time of hatching. These cells or organs do not seem to be specifically involved in the mechanisms of adjustment to the hypertonic osmotic stress at this time. The size of the nuclei of the prolactin cells increased in fresh-water (F. W.) and that of the ACTH cells decreased in 2/3, 1/2 and 1/3 sea-water (S.W.). The amount of chrome-alum haematoxylin-positive material in the neurohypophysis of fish immersed in 1/2 S.W., 1/3 S.W. and F.W. was less than that of the control fish. There was a difference in the glomerular ratio between the fish kept in F. W. and control fish. And the red blood cells in the glomerulus of the larvae kept in 1/2 and 1/3 S.W. apparently increased in number in comparison with the control fish.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 16
    ISSN: 1432-0878
    Schlagwort(e): Chloride cells ; Eel ; Gills ; Ionic transport ; Autoradiography
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Beschreibung / Inhaltsverzeichnis: Résumé Nous avons montré par une technique autoradiographique que les ions Cl− et Na+ sont concentrés dans les cellules à chlorure de la branchie d'anguille adaptée à l'eau de mer. La signification de cette accumulation ionique plus marquée vers le pôle apical de ces cellules a été discutée par rapport à l'excrétion branchiale de ces ions en eau de mer.
    Notizen: Summary With an autoradiographic technique Cl− and Na+ ions have been shown to be localized in the chloride cells of sea water eel gills. The significance of this accumulation, more marked towards the apical pole of these cells, is discussed with regard to branchial excretion of these ions in sea water.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 17
    Digitale Medien
    Digitale Medien
    Springer
    Cell & tissue research 143 (1973), S. 535-547 
    ISSN: 1432-0878
    Schlagwort(e): Adenohypophysis ; Rat ; Mitoses, topographic distribution ; Influence of fixation ; Light microscopy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Beschreibung / Inhaltsverzeichnis: Résumé Les mitoses de l'adénohypophyse du rat mâle ont été examinées comparativement après fixation par perfusion et après fixation par immersion. Après fixation par perfusion la répartition topographique des mitoses dans le parenchyme est homogène, et l'index mitotique est de 1,85 pour 1 000 cellules. Après fixation par immersion, la densité des mitoses décroit de la périphérie vers le centre, et l'index mitotique n'est que de 0,80 mitoses pour 1 000 cellules. La lenteur de pénétration du fixateur pendant l'immersion est tenue pour principal responsable des différences observées. Elle entraîne une modification de l'aspect des mitoses, telle que plus de la moitié d'entre elles ne sont pas identifiables et échappent aux comptages.
    Notizen: Summary The mitoses of the adenohypophysis in male rats have been observed comparatively after fixation by perfusion and fixation by immersion. After fixation by perfusion, the topographic distribution of mitoses throughout the parenchyma is homogenous and the mitotic index is 1.85 for 1 000 cells. After fixation by immersion, the density of the mitoses is decreasing from the periphery towards the center and the mitotic index is only 0.80 for 1 000 cells. The slow penetration of the fixative during fixation by immersion is considered to be the main cause in the differences observed. This slow action causes a modification in the aspect of the mitoses, and more than fifty percent of them cannot be identified and escape to the counts.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 18
    Digitale Medien
    Digitale Medien
    Springer
    Cell & tissue research 144 (1973), S. 353-368 
    ISSN: 1432-0878
    Schlagwort(e): Epidermis ; Bufo bufo ; Moulting cycle ; Structure ; Light microscopy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Skin samples were taken from 17 toads (Bufo bufo) in various phases of the moulting cycle. The phase was determined by recording a number of moulting intervals prior to sacrifice and by study of the macroscopic appearance of the skin and the moulting behaviour at sacrifice. Eight of the toads were in the moult when the samples were taken. Characteristic morphological changes were found to be restricted to a period immediately prior to and after shedding of the slough. Chemical changes of the membranes of stratum corneum (or material adhered to them) were observed already prior to adoption of the moulting posture. The separation from the underlying epidermis prior to shedding was accompanied by a swelling of the stratum corneum cells. After final detachment of the slough the replacement layer was differentiated into a new stratum corneum within 24 hours. Significant changes in the morphology of the flask cells were not observed. The findings are discussed with emphasis on the processes of separation and differentiation of the stratum corneum. Based on the morphology of the epidermis the following terminology is proposed for the phases of the moulting cycle: Intermoult phase, preparation phase, early shedding phase, late shedding phase, and differentiation phase.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 19
    Digitale Medien
    Digitale Medien
    Springer
    Cell & tissue research 142 (1973), S. 409-430 
    ISSN: 1432-0878
    Schlagwort(e): Oocyte ; Honeybee ; Vitellogenesis ; Yolk pattern ; Autoradiography
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Beschreibung / Inhaltsverzeichnis: Zusammenfassung Die Oogenese der Bienenkönigin (Apis mellifica) kann in 6 Stadien eingeteilt werden. Proteinsynthese und Dottereinlagerung in der Oocyte werden autoradiographisch und mittels Trypanblau-Vitalfärbung untersucht. Euplasmatische Proteinsynthesen finden in allen Eifollikeln bis zum frühen Stadium 5 statt. Vitellogenese-aktiv sind nur die Oogenese-Stadien 3 und 4. Die pinocytäre Einschleusung dotterpflichtiger Haemolymphproteine ins Ooplasma nimmt etwa 20 min in Anspruch. 10 min später sind kleinste neugebildete Dotterkugeln in der äußersten Oocytenperipherie zu erkennen. Aus ihnen werden binnen 1 $${\raise0.5ex\hbox{$\scriptstyle 1$}\kern-0.1em/\kern-0.15em\lower0.25ex\hbox{$\scriptstyle 2$}}$$ —2 h große Dotterschollen von ca. 20 μm φ. Die Vitellogenese-Phase eines einzelnen Follikels dauert insgesamt 3 Tage. Dotterschollen werden nur im Bereich des freien Follikelepithels gebildet. Sie wandern einzeln ins zentrale Ooplasma ein, wo unterschiedlich alte Schollen durchmischt abgelagert werden. Dotterfrei bleibt ein Euplasmahof, der den Vorderpol des Eies markiert. Im Ooplasma und in den Nährzellen erfolgt sicher keine Synthese von Dottermaterial. Ein Beitrag der Follikelepithelzellen zur Dotterbildung kann nicht vollständig ausgeschlossen werden. Das zeitliche und räumliche Muster der Dottereinlagerung wird diskutiert unter Berücksichtigung der Determination der Oocyten-Achsen und der Präformation von ooplasmatischen Kontrollzentren für die frühe Embryogenese.
    Notizen: Summary Oogenesis in the honeybee queen (Apis mellifica) can be divided into 6 developmental stages. By autoradiography and vital staining with trypan blue protein synthesis and yolk incorporation into the oocyte were studied. Euplasmic protein synthesis occurs in all egg follicles up to the early stage 5. Only the stages 3 and 4 are active in vitellogenesis. The resorption of vitellogenic haemolymph proteins by pinocytosis needs about 20 min. 10 min later very small newly formed yolk globules can be recognized in the outermost periphery of the oocyte. After 1 $${\raise0.5ex\hbox{$\scriptstyle 1$}\kern-0.1em/\kern-0.15em\lower0.25ex\hbox{$\scriptstyle 2$}}$$ —2 hrs large yolk platelets of ca. 20 μm in diameter arise from them. The whole vitellogenesis of a single follicle lasts 3 days. Yolk globules are formed only in the area of the free follicle epithelium. They migrate separately into the central ooplasm where yolk platelets of different age are deposited in a mixed pattern. An euplasmic halo remains free of yolk thus marking the anterior pole of the egg. It is shown that no yolk material is synthesized in the ooplasm and in the trophocytes. A contribution of the follicle epithelium to yolk formation can not totally be excluded. The temporal and spatial pattern of yolk deposition is discussed. The determination of the oocyte axis and the preformed ooplasmic reaction centers for early embryogenesis are considered.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 20
    Digitale Medien
    Digitale Medien
    Springer
    Cell & tissue research 136 (1973), S. 45-58 
    ISSN: 1432-0878
    Schlagwort(e): Chicken ; Bursa Fabricii ; Thymus ; Spleen ; Tonsilla caecalis ; Light microscopy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Beschreibung / Inhaltsverzeichnis: Zusammenfassung Bursa Fabricii, Thymus, Milz und Zäkaltonsillen von 63 Hühnern im Alter von 1 Tag bis zu 1 Jahr werden histologisch untersucht, wobei das Vorkommen der als bursaabhängig geltenden Keimzentren und Plasmazellen quantitativ erfaßt wird. Beide treten in der Milz und Tonsilla caecalis erst während des Reifestadiums der Bursa in zunehmender Menge auf. Mit Beginn der Bursainvolution werden Keimzentren und pyroninophile Zellen in der Milz selten, während in den Zäkaltonsillen kaum Veränderungen auftreten. Im Thymus verschiebt sich das Verhältnis zwischen Mark- und Rindenbreite zugunsten des Marks. Bei 8 Monate alten Tieren fehlt die Rinde vollständig. Plasmazellen treten im Thymusmark während des Reifestadiums der Bursa auf und nehmen mit dem Beginn deren Involution zu. In dem Zeitraum zwischen 2 Wochen und 5 Monaten ändert sich das Verhältnis zwischen reifen und unreifen Plasmazellen beständig zugunsten der reifen Plasmazellen.
    Notizen: Summary Bursa of Fabricius, thymus, spleen and cecal tonsils were histologically examined with special reference to the number of bursa-dependent germinal centers and plasma cells in 63 chickens, 1 day to 1 year old. Both structures appear in spleen and cecal tonsils increasingly during the bursal maturity stage. At the beginning bursal involution, germinal centers and pyroninophilic cells become rare in the spleen, in contrary no changes appear in the cecal tonsils. The medulla cortex ratio of the thymus changes in favour of the medulla, in animals 8 months of age the cortex is absent. Plasma cells appear in the medulla of the thymus during the bursal maturity stage and increase in number with beginning involution. During the period of 2 weeks to 5 months the proportion between mature and immature plasma cells changes in favour of mature cells.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 21
    Digitale Medien
    Digitale Medien
    Springer
    Cell & tissue research 139 (1973), S. 149-170 
    ISSN: 1432-0878
    Schlagwort(e): Suctorial disc ; Echeneis naucrates ; Sustentacular tissue ; Sense organs ; Light microscopy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Beschreibung / Inhaltsverzeichnis: Zusammenfassung Die lichtmikroskopische Untersuchung der Haftscheibe des Teleostiers Echeneis naucrates führte unter anderen zu folgenden Ergebnissen: 1. Das mehrschichtige, Schleimzellen enthaltende Epithel der Scheibe weist deutliche regionale Unterschiede auf. So ist auf der Dorsalfläche der flexiblen Randlippe eine an Schleimdrüsen besonders reiche bandförmige Zone ausgebildet. Ferner werden die Eingänge in die Taschen zwischen den Lamellen des Saugapparates und die Oberfläche der medianen Hautleiste von stark schleimbildendem Epithel umgeben bzw. bedeckt. Der Schleimstreifen der Haftscheibe wird als Einrichtung zur Abdichtung der Haftfläche gedeutet. 2. Im Drüsenfeld auf der Dorsalfläche der Randlippe liegen große Sinnesorgane vom Typus der Chemorezeptoren. Ihre Funktion besteht möglicherweise in der Prüfung der Oberfläche des Wirtes, an dem sich Echeneis festsaugt. Die Angaben von Houy (1910), wonach diese Sinnesorgane in der Wand eines Kanalsystems liegen und nicht intraepithelial, beruhen auf der Fehlinterpretation artifiziell entstandener Spalten. 3. Die interlamellären Taschen der Saugplatte werden von einem verhältnismäßig niedrigen und an Schleimzellen armen, gefältelten Epithel ausgekleidet. Es ist der Beanspruchung auf Dehnung angepaßt, die bei dem Ansaugen infolge Vertikalstellung der Lamellen der Scheibe auftritt. 4. Der ovale abgeplattete Stützgewebsstreifen innerhalb der Randlippe, dessen Gewebe von Houy (1910) nicht klassifiziert werden konnte, besteht aus hyalinzelligem Chondroidgewebe (Schaffer), das von kollagenen und elastischen Fasern, ferner von einem weitmaschigen Kapillarnetz durchsetzt wird. Zarte Septen aus Kollagenfilamenten umgeben jede einzelne Hyalinzelle. Das im Zentrum des Reifens gelegene univakuoläre Fettgewebe differenziert sich aus den Hyalinzellen des Chondroidgewebes, die ihrerseits von perichondralen Fibrozyten abstammen. Auch die innerhalb der Lamellen des Saugapparates gelegenen Knorpelformationen werden von hyalinzelligem Chondroidgewebe gebildet, das mit Fettgewebe in Zusammenhang steht. 5. Die Faserarchitektur des Randstreifens entspricht der trajektoriellen Bauweise des Hyalinknorpels.
    Notizen: Summary A light microscopical study of the suctorial disc of the teleost Echeneis naucrates revealed the following details: 1. The stratified mucous-cell bearing epithelium of the disc shows distinctive regional differences. Thus the dorsal surface of the flexible marginal lip contains a peculiar glandular zone which is particularly rich in mucous cells. Furthermore, the openings to the pockets between the lamellae of the suction apparatus and the surface of the median dermal ridge are mucus producing zones. These areas are interpreted as devices for tightening. 2. In the mucous zone of the dorsal surface of the marginal lip are large sensory organs of chemoreceptor type. Possibly their function consists in the chemical assessment of the host's surface. The statement by Houy (1910) according to which these organs are located in the wall of a canalicular system rather than intraepithelially stems from his erroneous interpretation of artifactitious clefts. 3. The interlamellar pockets of the suction disc are lined by a low folded epithelium with relatively few mucous cells. It is capable of adjusting to the stretch caused by the tilting of the disc's lamellae during suction. 4. The oval flattened ring of sustentacular tissue within the marginal lip, which Houy (1910) was unable to classify, represents a chondroid tissue consisting mainly of hyaline cells (“hyalinzelliges Chondroidgewebe, Schaffer 1930”) and traversed by collagenous and elastic fibres. It also contains a wide-meshed capillary network. Each hyaline cell is surrounded by delicate septa formed by collagenous filaments. The unilocular adipose tissue in the ring's center develops from the hyaline cells of the chondroid tissue which in turn are derived from perichondrial fibrocytes. Also the cartilaginous structures within the lamellae of the suction apparatus are formed by the same chondroid tissue that is in connection with adipose tissue. 5. The spatial arrangement of the fibres and fibrils traversing the sustentacular tissue within the marginal lip corresponds to the trajectorial pattern of hyaline cartilage.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 22
    Digitale Medien
    Digitale Medien
    Springer
    Cell & tissue research 137 (1973), S. 313-329 
    ISSN: 1432-0878
    Schlagwort(e): Nervous system ; Limulus polyphemus ; Abdominal ganglion ; Neurons ; Light microscopy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The morphology of the first abdominal ganglion of Limulus polyphemus (L.) was examined by reconstructions from stained serial sections. This ganglion, one of a chain of eight which form the ventral cord, is bilaterally symmetrical and shows the typical invertebrate division into two zones, cellular rind and fibrous core. The core of the ganglion comprises two major longitudinal tracts which are continuous with the connectives between the ganglia and four areas of regional neuropile which occur at the roots of the nerves developed from the ganglion. Minor tracts and neuropile are present. The rind is divided into several cell groups: U-shaped lobes around the nerve roots, a lateral lobe between the nerve roots and two cell layers along the ventral aspect. Six cell types are recognized: encapsulated unipolar cells, secretory unipolar cells (two types), encapsulated bipolar cells, “neuroglandular” bipolar cells and small non-encapsulated cells.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 23
    Digitale Medien
    Digitale Medien
    Springer
    Cell & tissue research 140 (1973), S. 509-534 
    ISSN: 1432-0878
    Schlagwort(e): Genital tract ; Viviparous toad ; Cyclic variations ; Light microscopy ; Biometry
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Beschreibung / Inhaltsverzeichnis: Résumé L'oviducte de Nectophrynoïdes occidentalis Angel, petit crapaud vivipare d'Afrique occidentale, peut être divisé en quatre parties: le pavillon, la trompe, l'utérus et le tronc commun utérin distal. Seul le pavillon ne varie pas au cours de l'année; il reste toujours transparent et dépourvu de cellules glandulaires. Un épithélium cilié tapisse sa lumière. La trompe est un conduit rectiligne de 7 à 10 millimètres de long qui peut être divisé en quatre segments principaux, différenciés par la constitution chimique des substances de la muqueuse. La structure de la trompe est relativement simple et comparable à celle des autres Vertébrés inférieurs. Elle apparait alternativement sous deux aspects principaux: trompe au repos et trompe active. La période de repos se poursuit pendant une bonne partie de la gestation; la reprise d'activité sécrétrice s'effectue plusieurs semaines avant la parturition et atteint son maximum dans les jours qui précèdent l'ovulation. L'utérus subit continuellement des remaniements. Tour à tour vide et gravide, il passe par trois phases essentielles: une phase de nécrose s'étendant sur les douze jours qui suivent la parturition et correspondant à l'élimination de la couche superficielle de la muqueuse; une phase de prolifération, allant du douzième jour après la mise-bas jusqu'à lóvulation suivante; caractérisée par le régénération progressive de la muqueuse à partir de la couche profonde restée en dehors des processus de nécrose; une phase de sécrétion et d'hypérémie qui dure pendant toute la gestation. La partie terminale de l'oviducte, formée par la confluence des deux conduits müllériens, subit les mêmes variations que l'utérus gestant. Son épithélium est tout-à-fait comparable à celui de l'utérus; son chorion, en revanche, est beaucoup plus dense et développé mais ne possède qu'une faible vascularisation.
    Notizen: Summary The oviduct of Nectophrynoides occidentalis Angel, a small viviparous toad of West Africa, is divided into four parts: ostium, tube, uterus and the distal common uterine segment. Only the ostium does not show seasonal variations: it always remains transparent and is devoid of glandular cells. A ciliated epithelium lines its lumen. The tube is rectilinear, about 7–10 millimeters long and may be subdivided into four principal segments, on the basis of the chemical composition of the substances of the mucosa. Its structure is relatively simple and comparable to that of other lower vertebrates. Its appearance differs according to whether it is at rest or in activity. For most of the gestation period the tube is at rest but secretory activity starts several weeks before birth. It reaches its maximum during the days immediately preceding ovulation. Unlike the tube, the uterus undergoes continuous changes, which can be pregnant, divided into three essential phases: a phase of necrosis, extending to twelve days following birth, and corresponding to the elimination of the superficial layer of the mucosa; a phase of proliferation, extending from the twelfth day after birth until the subsequent ovulation, characterized by the progressive regeneration of the mucosa, which is initiated by the deep layer which was not involved in necrosis; a phase of secretion and hyperemia which lasts during the whole period of gestation. The terminal part of the oviduct, formed by the confluence of the two Müllerian ducts, undergoes the same variations as the uterus, its epithelium being completely comparable to that of the uterus; its chorion, however, is much denser and better developed but its vascularization is less intense.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 24
    Digitale Medien
    Digitale Medien
    Springer
    Cell & tissue research 141 (1973), S. 93-101 
    ISSN: 1432-0878
    Schlagwort(e): Optic system (Teleosts) ; Gangliosides and Sialo-glycoproteins ; Site of synthesis ; Autoradiography
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Beschreibung / Inhaltsverzeichnis: Zusammenfassung Der Einbau von intracranial sowie intraocular-appliziertem N-acetyl-3H-Mannosamin (N-Ac-3H-Man) in das optische System (Tectum opticum bzw. Tractus opticus) von Scardinius erythrophthalmus und Carassius carassius (cyprinidae, Telosteer) wurde autoradiographisch untersucht und mit dem Einbau von 3H-Histidin verglichen. 1. Intracranial appliziertes N-Ac-3H-Man wird im Tectum opticum in Glykoproteine und Ganglioside im Verhältnis von 2:1 eingebaut. 2. Die Synthese von Gangliosiden und Sialo-Glycoproteinen erfolgt im Nervengewebe im Gegensatz zu der von Proteinen ubiquitär, d. h. sowohl in den Perikaryen als auch in den Fasern. 3. Nach einseitiger intraocularer Applikation von N-Ac-3H-Man werden bereits nach 24 stündiger Inkorporationszeit ausschließlich die mit dem injizierten Auge zusammenhängenden Sehschichten (Stratum opticum) des kontralateralen Tectum opticum radioaktiv markiert. Im Zusammenhang mit ergänzenden biochemischen Analysen ergibt sich, daß die Radioaktivität niedermolekularer Aminozucker mit dem schnellen axonalen Transport (〉40 mm/d) zum Zwecke einer peripheren Gangliosid-Synthese im Tractus opticus proximo-distal verlagert wird.
    Notizen: Summary The incorporation of intra-cranially and intra-ocularly injected (N-Ac-3H-Man) and 3H-histidine into the optic systems (optic tectum and optic tract) of Scardinius erythrophthalmus and Carassius carassius was investigated by means of autoradiography. 1. N-Ac-3H-Man injected intra-cranially, is incorporated into gangliosides and glycorproteins in a ratio of 1:2 in the optic tectum. 2. In nervous tissue gangliosides and sialo-glycoproteins are labeled ubiquitously, i.e. in the perikarya as well as in the fibers. 3. Following an intra-ocular injection of N-Ac-3H-Man and after an incorporation period of 24 hrs, only the optic layers in the contralateral optic tectum are labelled (gangliosides). Together with corresponding biochemical data, this shows, that in the optic tract radioactivity of low molecular amino sugars is transported proximo-distally by means of rapid axonal flow (〉40 mm/d), before the incorporation into high molecular compounds.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 25
    Digitale Medien
    Digitale Medien
    Springer
    Cell & tissue research 146 (1973), S. 309-317 
    ISSN: 1432-0878
    Schlagwort(e): Adrenal cortex ; Prostaglandin E1 ; Autoradiography ; Electron microscopy ; Lipid analysis
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary To determine whether or not prostaglandins enter adrenocortical parenchymal cells,3H-PGE1 was injected intravenously into rats. In histological preparations, grains denoting activity were noted in intracellular lipid droplets and nuclei and in sinusoids. At the fine structural level, activity was observed in lipid droplets, mitochondria, the agranular endoplasmic reticulum, nuclei and the plasma membrane. Biochemical lipid analyses of the adrenals revealed activity in the cholesterol and cholesterol ester fractions. Large amounts of unaltered3H-PGE1 and its degradation products were also present. Compared to the liver, the adrenal was more effective in degrading prostaglandin, when expressed on a weight basis. The possible roles of the organelles in PGE1 degradation and in prostaglandin-related hormone synthesis are discussed.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 26
    Digitale Medien
    Digitale Medien
    Springer
    Cell & tissue research 146 (1973), S. 1-14 
    ISSN: 1432-0878
    Schlagwort(e): Ventral cord ganglia ; Limulus polyphemus ; Cell types ; Neurosecretion ; Cytochemistry ; Light microscopy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The ventral cord ganglia of the horseshoe crab, Limulus polyphemus, contains six distinct cell types: three appear to be ordinary neurons and three exhibit the staining affinities of neurosecretory cells. The presumed neurosecretory cells have been termed neurosecretory cell I (NSC I), NSC II and NSC III. NSC I cells contain a colloid-like inclusion which may occur as a single small vacuole or occupy more than one-half of the cell volume. Colloid inclusions occur with greater frequency toward the periphery, although small cells of similar staining affinity occur in cords extending to the fibrous core. The histochemical tests suggest that the cytoplasm is positive for proteins, but contains no strong acidic groups which may have been derived from S-S or S-H groups. The presence of carbohydrate is also indicated. NSC II cells exhibit distinct secretory cycles. Early in the cycle the cytoplasm becomes phloxinophilic and progresses to a distinct fuchsinophilic stage. Small homogeneous irregular inclusions are found in the axon hillock during the latter stages of the cycle. Histochemical tests suggest the presence of a carbohydrate and strong acidic groups which may have been derived from S-S or S-H groups. There are small cells present which appear to be immature neurosecretory cells. NSC III cells are characterized by a perinuclear ring of cytoplasm which is stained by chrome alum hematoxylin but not by paraldehyde fuchsin. A secretory cycle may also be present in this cell type. The three cell types presumed to be ordinary neurons exhibit no particular staining affinity for the stains or tests used in this study.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 27
    Digitale Medien
    Digitale Medien
    Springer
    Cell & tissue research 146 (1973), S. 261-279 
    ISSN: 1432-0878
    Schlagwort(e): Skeletal muscle ; Rat, Cat ; Motor endplate ; Reinnervation ; Light microscopy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary 1. The formation of endplates outside the original endplate region of a muscle fibre was studied in slow and fast rat muscles. It was found that such new endplates are readily formed on the soleus muscle, whereas hardly at all in the fast extensor digitorum longus. Most new endplates appear to be morphologically normal within 2 months after nerve implantation. 2. The time course of recovery of slow and fast cat muscles was followed after crushing the sciatic nerve. It was found that the slow soleus muscle recovers more rapidly than the fast flexor hallucis longus muscle. 3. The endplates of reinnervated cat muscles are more complicated than those of the control muscles, but have nevertheless fewer nerve terminals per endplate. Reinnervated muscles are more sensitive to curare and it is suggested that this is due to a decrease in transmitter release, for it was found that less acetylcholine is released from reinnervated rat hemidiaphragms than from control ones. 4. Motor and sensory reinnervation of spindles and tendon organs was studied. At the time when motor reinnervation is almost completed the sensory endings from spindles and tendon organs are highly abnormal. Thus sensory reinnervation proceeds much more slowly than motor.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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