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  • 1
    Digitale Medien
    Digitale Medien
    Springer
    Calcified tissue international 12 (1973), S. 169-173 
    ISSN: 1432-0827
    Schlagwort(e): Calcification ; Histochemistry ; Autoradiography ; Fetal ; Mandible
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin , Physik
    Beschreibung / Inhaltsverzeichnis: Résumé Des rats femelles reçoivent du45Ca ou du32P au 13ème et 18ème jour de la grossesse. Les coupes sériées des têtes de foetus sont étudiées par autoradiographie et par diverses méthodes histologiques pour déterminer la calcification. La détection la plus précoce de45Ca s'observe simultanément comme une réaction positive pour le calcium avec une des méthodes histologiques utilisées.32P est en évidence par les méthodes autoradiographiques un peu plus tard que le45Ca et sa présence coincide avec la réaction positive la plus précoce observée avec les autres méthodes histologiques utilisées. Les os de ces têtes foetales commencent à se calcifier selon un mode particulier commençant par la mandibule, puis l'os frontal et le maxillaire supérieur, suivis par les os nasaux, pariétaux et interpariétaux.
    Kurzfassung: Zusammenfassung Weibliche Ratten erhielten45Ca und32P zwischen dem 13.–18. Tag der Schwangerschaft. Zur Ermittlung der Verkalkung wurden Serienschnitte der Köpfe ihrer Feten mittels Autoradiographie sowie durch verschiedene histologische Methoden untersucht. Das erste Auftreten von45Ca war ebenfalls von einer positiven Calciumreaktion mittels einer der verwendeten histologischen Methoden begleitet.32P wurde in der Autoradiographie erst etwas später als45Ca festgestellt und dessen Nachweis deckte sich mit den ersten positiven Reaktonen aller anderen verwendeten histologischen Methoden. Die Knochen in diesen Fetusköpfen begannen in einer bestimmten Sequenz zu verkalken: zuerst der Unterkiefer, dann das Stirnbein und der Oberkiefer, dann das Nasenbein, die Parietal- und Interparietalknochen.
    Notizen: Abstract Female rats were given45Ca or32P from 13 to 18 days of pegnancy. Serial sections from the heads of their fetuses were studied by autoradiography, as well as by several histological methods for assessing calcification. The earliest detection of45calcium occured at the same time as a positive reaction for calcium with one of the histological methods used.32P was not detectable by autoradiographic methods until somewhat later than45Ca, and its presence coincided with the earliest positive reaction with all of the other histological methods employed. The bones in these fetal heads began to calcify in a partcular sequence, the mandible first, then the frontal bone and maxilla, followed by the nasal, parietal and interparietal bones.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Springer
    Archives of microbiology 139 (1984), S. 218-220 
    ISSN: 1432-072X
    Schlagwort(e): Thioploca ; Nutrition ; Autoradiography ; Mixotrophy ; Methylotrophy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Autoradiographic evidence showed strong incorporation by two marine thioplocas of 14C from acetate, amino acids, carbonate, glucose and glycinc and of 3H from thymidine. Methane, methanol and glutamate were not incorporated. Since the data suggested that sulfide was needed for strong incorporation, a mixotrophic nutrition was indicated.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 3
    Digitale Medien
    Digitale Medien
    Springer
    Cell & tissue research 139 (1973), S. 83-94 
    ISSN: 1432-0878
    Schlagwort(e): Subcommissural organ ; Reissner's fibre ; Rana esculenta ; Light/Darkness ; Autoradiography
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The influence of long daily photoperiods and of continuous darkness on the secretory activity of the subcommissural organ (SCO) and on the growth rate of Reissner's fibre (RF) in Rana esculenta was studied in winter. The amount of aldehyde-fuchsin (AF) positive material in the apical cell parts of the SCO is smaller in light-adapted than in darkness-adapted animals. Differences in incorporation rate of 35S-cysteine between the SCO of light-adapted and of darkness-adapted animals, however, are small. The conclusion must be that light/darkness conditions only slightly influence the secretory activity of the SCO cells of Rana esculenta. In agreement with this conclusion is the observation that RF grew only slightly faster in the light-adapted than in the darkness-adapted animals. Under the conditions used RF was completely renewed in about 3 to 4 days. Influences of temperature on the activity of the SCO have been observed.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 4
    Digitale Medien
    Digitale Medien
    Springer
    Cell & tissue research 139 (1973), S. 179-200 
    ISSN: 1432-0878
    Schlagwort(e): Retino-preoptic pathway ; Optic tracts ; Rana temporaria ; Light microscopy ; Autoradiography
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The optic tracts and centres of optic terminals of Rana temporaria have been investigated with silver impregnation techniques after unilaterally cutting the optic nerve and autoradiographically after injection of a mixture of tritiated amino acids into the vitreous body of the left eye. The observations on the course of the optic tracts and on the optic terminals in the thalamus and optic tectum are to a great extent in agreement with those of other authors. The probability of a retino-preoptic pathway is supported by the detection of fibres running in dorsal direction in front of the place where the optic nerve penetrates the brain. In horizontal sections these fibres can be seen deviating from the optic tract, covering a short distance in frontal direction and then turning upward under an angle of about 90 degrees. They disappear between the ventral aldehyde-fuchsin positive cells of the preoptic nucleus. The ventral and median parts of the ipsilateral preoptic nucleus contain fragments of degenerated fibres. Autoradiographic data are also in favour of the presence of an ipsilateral retino-preoptic tract. After carefully counting the number of grains over left and right preoptic nucleus, it appeared that over the ventral and median parts of the left preoptic nucleus more grains occur than over the identical areas of the right one.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 5
    Digitale Medien
    Digitale Medien
    Springer
    Cell & tissue research 136 (1973), S. 355-364 
    ISSN: 1432-0878
    Schlagwort(e): Hypothalamo-hypophysial system ; Rana temporaria ; Blinding ; Illumination ; Autoradiography
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The influence of a long daily photoperiod and continuous darkness on the hypothalamo-hypophysial system of blinded frogs was studied. In the preoptic nucleus no difference could be observed between the two groups of blinded frogs neither in the amount of incorporated 35S-cysteine nor in the amount of aldehydefuchsin positive material nor in the nuclear volume of the secretory cells. These results were compared with those of an experiment with intact frogs kept simultaneously under the same environmental conditions. It appeared that the activity of the dark-treated blinded group was enhanced compared with the activity of the dark-treated intact group while the light-treated blinded frogs showed a decrease in activity compared with the similarly treated intact animals. Likewise the influence of a long daily photoperiod on the hypothalamo-hypophysial system of unilaterally blinded frogs was studied. The activity of the preoptic nucleus was lower on the side where the optic nerve had been cut than on the intact side. In the dorsal part of the preoptic nucleus a difference between the two sides could not be observed. The significance of these results is discussed with regards to a possible connection between retina and preoptic nucleus.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 6
    ISSN: 1432-0878
    Schlagwort(e): Neurosecretion ; Annelida ; Sexuality and Development ; Autoradiography
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Beschreibung / Inhaltsverzeichnis: Zusammenfassung Während der sexuellen Entwicklung vonPlatynereis dumerilii steigt die Zahl der Neurone, die Merkmale einer sekretorischen Aktivität aufweisen, parallel zum Wachstum und zur Reifung der Keimzelle an. Die cytologische Differenzierung eines Neurons zur neurosekretorischen Zelle ist mit einer Steigerung der Synthese Cystin-haltigen Materials gegenüber konventionellen Neuronen gekoppelt. Neurosekretorische Zellen treten erstmals in Erscheinung, kurz bevor die Oocyten in die zweite Oogeniephase eintreten, während der die Vitellogenese abläuft. Aufgetragen gegen den Oocytendurchmesser ergibt die Zahl der Neurone, die sekretorisch aktiv werden und3H-Cystin inkorporieren, eine ansteigende Kurve. Die Kurve biegt auf ein Plateau, wenn die Oocyten eine kritische Größe erreichen. Diese Größe markiert jenes Entwicklungsstadium, von dem ab eine Enthirnung eine normale Reifung nicht mehr verhindert. Die Zahl synthetisch tätiger Neurone steight erneut an, wenn die Oocyten die kortikale Struktur des reifen Eies entwickeln. Das Maximum wird kurz vor der Metamorphose erreicht. Während der epitoken Umwandlung sinkt die synthetische Aktivität rasch ab, um im schwärmbereiten Tier gänzlich zum Stillstand zu kommen. In Tieren, die nach der kritischen Phase zur caudalen Regeneration veranlaßt werden, fällt die Zahl markierter neurosekretorischer Zellen auf ein Niveau ab, das für junge, „vorkritische“ Individuen charakteristisch ist. Entsprechend wird in jungen, vorkritischen Tieren, die erst wenige endokrine Neurone besitzen, die Differenzierung weiterer neurosekretorischer Zellen durch eine Regeneration nicht stimuliert. Der zeitliche Verlauf der synthetischen Aktivität im Zuge der Entwicklung und die durch eine Regeneration erzwungenen Veränderungen sind somit reziprok zur Aktivität des Gehirns in Bezug auf die Ausschüttung des Juvenilhormons. Die Hypothese wird zur Diskussion gestellt, die sinkende Produktion des Juvenilhormons werde von der steigenden Produktion eines antagonistischen neuroendokrinen Prinzips begleitet oder verursacht.
    Notizen: Summary During sexual development ofPlatynereis dumerilii, the number of neurones exhibiting secretory activity rises in parallel with the growth and maturation of the germ cells. The cytological differentiation of neuroglandular properties is accompanied with an increased uptake of tritiated cystine. The first neurones developing the characteristics of secretory cells and incorporating cystine appear just before the oocytes enter the second growth phase which corresponds with vitellogenesis. When plotted against the oocyte diameter the number of labelled neurosecretory cells yields an ascending line, which becomes a plateau once the oocytes approach a critical size. This critical size defines the developmental stage beyond which decerebration does not prevent normal maturation. The neuronal synthetic activity rises again when the oocytes differentiate the cortical structure of ripe eggs. The curve displays its maximum slope at the onset of metamorphosis. During the epitokous transformation the synthetic activity decreases rapidly and is almost totally depressed when swarming is imminent. In animals induced to regenerate their posterior segments after they have passed the critical stage, the number of labelled neurosecretory cells becomes reduced to the precritical level. According to this, regeneration in young precritical animals containing only few endocrine neurones does not stimulate the development of further secretory neurones. Thus, the time course during maturation and the changes caused by regeneration are reciprocal to the activity of the brain with respect to the release of juvenile hormone. The question is thus raised as to whether the declining titer of juvenile hormone may be accompanied or caused by an increased production of an antagonistic principle related to maturation.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 7
    Digitale Medien
    Digitale Medien
    Springer
    Cell & tissue research 146 (1973), S. 491-500 
    ISSN: 1432-0878
    Schlagwort(e): Hypothalamo-hypophysial system ; Pineal complex ; Rana esculenta ; Light/darkness ; Autoradiography
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary InRana esculenta the activity of the hypothalamo-hypophysial system has been investigated in intact and blinded animals, after removal of the frontal organ and after elimination of the whole pineal complex. The results on intact and blinded animals are practically the same as those found earlier inRana temporaria. Light increases the activity of the preoptic nucleus, blinding changes the difference between light- and darkness-treated intact animals. Compared with the intact animals the other two groups (without frontal organ and without pineal complex) show a marked increase in incorporation of35S-cysteine in the preoptic nucleus. A difference between the animals without frontal organ and those without pineal complex, however, can hardly be observed. It follows that the absence of the frontal organ has the same influence on the preoptic nucleus as elimination of the whole pineal complex. In intact animals the influence of the frontal organ on the preoptic nucleus consists of an inhibition of neurosecretory activity as well under light as under darkness conditions. The enhanced synthesis of labelled material in the preoptic nucleus after removal of the frontal organ, results in a transport along the hypothalamo-hypophysial tract mainly to the outer zone of the median eminence and not to the posterior lobe.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 8
    Digitale Medien
    Digitale Medien
    Springer
    Cell & tissue research 139 (1973), S. 431-450 
    ISSN: 1432-0878
    Schlagwort(e): Cardiac muscle ; Frog ; Regeneration ; Electron microscopy ; Autoradiography
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary From the 5th day up to the end of 3rd week following local crushing of the frog ventricle myocardium, ca. 13% of myocyte nuclei, in the vicinity of the damaged zone, were labelled after a single 3H-thymidine (3HTdr) injection, and 30–50% of these were labelled after repeated 3HTdr administration. The number of myocyte mitoses was maximal (ca. 1.3%) at the beginning of the 3rd week. The reactive proliferation of myocytes was accompanied by their “partial dedifferentiation”. This involved the nuclear euchromatic rearrangement, increase in size of nuclei and nucleoli, accumulation of the sarcoplasm rich in free ribosomes and rough endoplasmic reticulum, hyperplasia of the Golgi apparatus, and the appearance of 80–100 Å in diameter cytofilaments. Electron microscope autoradiography has shown that all these changes may be more or less pronounced in myocytes incorporating 3HTdr. The myofibril ultrastructure was found to be unchanged during S phase. However, in the mitotically dividing myocytes, the majority of Z-disks were disintegrated resulting in progressive release of myofilament bundles. Both 3HTdr labelled and mitotic myocytes were anchored to the adjacent ones by desmosomes and intercalated disks. No free myoblasts were observed.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 9
    ISSN: 1432-0878
    Schlagwort(e): Chloride cells ; Eel ; Gills ; Ionic transport ; Autoradiography
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Beschreibung / Inhaltsverzeichnis: Résumé Nous avons montré par une technique autoradiographique que les ions Cl− et Na+ sont concentrés dans les cellules à chlorure de la branchie d'anguille adaptée à l'eau de mer. La signification de cette accumulation ionique plus marquée vers le pôle apical de ces cellules a été discutée par rapport à l'excrétion branchiale de ces ions en eau de mer.
    Notizen: Summary With an autoradiographic technique Cl− and Na+ ions have been shown to be localized in the chloride cells of sea water eel gills. The significance of this accumulation, more marked towards the apical pole of these cells, is discussed with regard to branchial excretion of these ions in sea water.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 10
    Digitale Medien
    Digitale Medien
    Springer
    Cell & tissue research 142 (1973), S. 409-430 
    ISSN: 1432-0878
    Schlagwort(e): Oocyte ; Honeybee ; Vitellogenesis ; Yolk pattern ; Autoradiography
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Beschreibung / Inhaltsverzeichnis: Zusammenfassung Die Oogenese der Bienenkönigin (Apis mellifica) kann in 6 Stadien eingeteilt werden. Proteinsynthese und Dottereinlagerung in der Oocyte werden autoradiographisch und mittels Trypanblau-Vitalfärbung untersucht. Euplasmatische Proteinsynthesen finden in allen Eifollikeln bis zum frühen Stadium 5 statt. Vitellogenese-aktiv sind nur die Oogenese-Stadien 3 und 4. Die pinocytäre Einschleusung dotterpflichtiger Haemolymphproteine ins Ooplasma nimmt etwa 20 min in Anspruch. 10 min später sind kleinste neugebildete Dotterkugeln in der äußersten Oocytenperipherie zu erkennen. Aus ihnen werden binnen 1 $${\raise0.5ex\hbox{$\scriptstyle 1$}\kern-0.1em/\kern-0.15em\lower0.25ex\hbox{$\scriptstyle 2$}}$$ —2 h große Dotterschollen von ca. 20 μm φ. Die Vitellogenese-Phase eines einzelnen Follikels dauert insgesamt 3 Tage. Dotterschollen werden nur im Bereich des freien Follikelepithels gebildet. Sie wandern einzeln ins zentrale Ooplasma ein, wo unterschiedlich alte Schollen durchmischt abgelagert werden. Dotterfrei bleibt ein Euplasmahof, der den Vorderpol des Eies markiert. Im Ooplasma und in den Nährzellen erfolgt sicher keine Synthese von Dottermaterial. Ein Beitrag der Follikelepithelzellen zur Dotterbildung kann nicht vollständig ausgeschlossen werden. Das zeitliche und räumliche Muster der Dottereinlagerung wird diskutiert unter Berücksichtigung der Determination der Oocyten-Achsen und der Präformation von ooplasmatischen Kontrollzentren für die frühe Embryogenese.
    Notizen: Summary Oogenesis in the honeybee queen (Apis mellifica) can be divided into 6 developmental stages. By autoradiography and vital staining with trypan blue protein synthesis and yolk incorporation into the oocyte were studied. Euplasmic protein synthesis occurs in all egg follicles up to the early stage 5. Only the stages 3 and 4 are active in vitellogenesis. The resorption of vitellogenic haemolymph proteins by pinocytosis needs about 20 min. 10 min later very small newly formed yolk globules can be recognized in the outermost periphery of the oocyte. After 1 $${\raise0.5ex\hbox{$\scriptstyle 1$}\kern-0.1em/\kern-0.15em\lower0.25ex\hbox{$\scriptstyle 2$}}$$ —2 hrs large yolk platelets of ca. 20 μm in diameter arise from them. The whole vitellogenesis of a single follicle lasts 3 days. Yolk globules are formed only in the area of the free follicle epithelium. They migrate separately into the central ooplasm where yolk platelets of different age are deposited in a mixed pattern. An euplasmic halo remains free of yolk thus marking the anterior pole of the egg. It is shown that no yolk material is synthesized in the ooplasm and in the trophocytes. A contribution of the follicle epithelium to yolk formation can not totally be excluded. The temporal and spatial pattern of yolk deposition is discussed. The determination of the oocyte axis and the preformed ooplasmic reaction centers for early embryogenesis are considered.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 11
    Digitale Medien
    Digitale Medien
    Springer
    Cell & tissue research 141 (1973), S. 93-101 
    ISSN: 1432-0878
    Schlagwort(e): Optic system (Teleosts) ; Gangliosides and Sialo-glycoproteins ; Site of synthesis ; Autoradiography
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Beschreibung / Inhaltsverzeichnis: Zusammenfassung Der Einbau von intracranial sowie intraocular-appliziertem N-acetyl-3H-Mannosamin (N-Ac-3H-Man) in das optische System (Tectum opticum bzw. Tractus opticus) von Scardinius erythrophthalmus und Carassius carassius (cyprinidae, Telosteer) wurde autoradiographisch untersucht und mit dem Einbau von 3H-Histidin verglichen. 1. Intracranial appliziertes N-Ac-3H-Man wird im Tectum opticum in Glykoproteine und Ganglioside im Verhältnis von 2:1 eingebaut. 2. Die Synthese von Gangliosiden und Sialo-Glycoproteinen erfolgt im Nervengewebe im Gegensatz zu der von Proteinen ubiquitär, d. h. sowohl in den Perikaryen als auch in den Fasern. 3. Nach einseitiger intraocularer Applikation von N-Ac-3H-Man werden bereits nach 24 stündiger Inkorporationszeit ausschließlich die mit dem injizierten Auge zusammenhängenden Sehschichten (Stratum opticum) des kontralateralen Tectum opticum radioaktiv markiert. Im Zusammenhang mit ergänzenden biochemischen Analysen ergibt sich, daß die Radioaktivität niedermolekularer Aminozucker mit dem schnellen axonalen Transport (〉40 mm/d) zum Zwecke einer peripheren Gangliosid-Synthese im Tractus opticus proximo-distal verlagert wird.
    Notizen: Summary The incorporation of intra-cranially and intra-ocularly injected (N-Ac-3H-Man) and 3H-histidine into the optic systems (optic tectum and optic tract) of Scardinius erythrophthalmus and Carassius carassius was investigated by means of autoradiography. 1. N-Ac-3H-Man injected intra-cranially, is incorporated into gangliosides and glycorproteins in a ratio of 1:2 in the optic tectum. 2. In nervous tissue gangliosides and sialo-glycoproteins are labeled ubiquitously, i.e. in the perikarya as well as in the fibers. 3. Following an intra-ocular injection of N-Ac-3H-Man and after an incorporation period of 24 hrs, only the optic layers in the contralateral optic tectum are labelled (gangliosides). Together with corresponding biochemical data, this shows, that in the optic tract radioactivity of low molecular amino sugars is transported proximo-distally by means of rapid axonal flow (〉40 mm/d), before the incorporation into high molecular compounds.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 12
    Digitale Medien
    Digitale Medien
    Springer
    Cell & tissue research 146 (1973), S. 309-317 
    ISSN: 1432-0878
    Schlagwort(e): Adrenal cortex ; Prostaglandin E1 ; Autoradiography ; Electron microscopy ; Lipid analysis
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary To determine whether or not prostaglandins enter adrenocortical parenchymal cells,3H-PGE1 was injected intravenously into rats. In histological preparations, grains denoting activity were noted in intracellular lipid droplets and nuclei and in sinusoids. At the fine structural level, activity was observed in lipid droplets, mitochondria, the agranular endoplasmic reticulum, nuclei and the plasma membrane. Biochemical lipid analyses of the adrenals revealed activity in the cholesterol and cholesterol ester fractions. Large amounts of unaltered3H-PGE1 and its degradation products were also present. Compared to the liver, the adrenal was more effective in degrading prostaglandin, when expressed on a weight basis. The possible roles of the organelles in PGE1 degradation and in prostaglandin-related hormone synthesis are discussed.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 13
    ISSN: 1432-0878
    Schlagwort(e): Ornithine decarboxylase ; Polyamines ; Mouse ; kidney ; Autoradiography
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Ornithine decarboxylase, a key enzyme in polyamine biosynthesis and cell growth, has been localized in mouse kidney by autoradiography after administration of radiolabeled α-difluoromethylornithine. This drug is an enzyme-activated irreversible inhibitor of ornithine decarboxylase and forms a covalent bond with the enzyme. It was found that ornithine decarboxylase is present in all cell types studied but that the highest content occurs in the proximal convoluted tubules followed by the distal convoluted tubules and the collecting tubules. The majority of the enzyme is located in the cytoplasm but about 10–15% is present in the nuclei (often associated with nucleolus-like components) of the cells of the proximal and distal convoluted tubules. The labeled ornithine decarboxylase was lost rapidly from both nucleus and cytoplasm of all the cell types examined, and labeling by radioactive α-difluoromethylornithine was greatly reduced if the mice were pretreated for 5 h with cycloheximide to block protein synthesis. These results indicate that ornithine decarboxylase turns over rapidly in all of the cells.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 14
    Digitale Medien
    Digitale Medien
    Springer
    Cell & tissue research 236 (1984), S. 661-667 
    ISSN: 1432-0878
    Schlagwort(e): Lymphocytes ; Spleen ; Autoradiography
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Male Wistar rats were injected intravenously with 5-(3H)uridine-labeled lymphocytes isolated from lymph nodes of syngeneic donors and enriched in T cells. After short periods of time (3 to 120 min after injection), labeled lymphocytes were localized in spleen compartments using autoradiography to identify routes of lymphocyte movement from blood into splenic parenchyma and to follow migration pathways of recirculating lymphocytes within the periarterial lymphoid sheath (PALS). Topographical analysis of labeled lymphocytes was performed in specific planes of PALS characterized by the diameter of the arterial vessel and termed PALS large, PALS medium, and PALS small (PALS L, PALS M, PALS S, respectively). Attention was also paid to accumulations of labeled lymphocytes close to the arterial vessel wall. Initially, labeled lymphocytes were localized in PALS S and PALS M near the terminal branching of arterial vessels and in the marginal zone (MZ). We conclude that lymphocytes emigrate from blood into splenic parenchyma within two white pulp compartments: in MZ, and directly within PALS through the wall of capillary vessels. The sequential accumulation of labeled cells near arterial vessels of increasing diameter suggests that the recirculating pool of lymphocytes migrates into the central part of PALS L by two routes: from MZ, and along arterial vessels from PALS S and PALS M.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 15
    Digitale Medien
    Digitale Medien
    Springer
    Cell & tissue research 238 (1984), S. 183-189 
    ISSN: 1432-0878
    Schlagwort(e): Lymphocyte migration ; Germinal centers ; Autoradiography ; Lymph nodes ; Lymphatic vessels ; B-lymphocytes, differentiation
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Affinity of lymphoid cells for the microenvironment of germinal centers (GC), as detectable in transfer experiments by rapid homing in spleen GC from the blood, is a capacity expressed by only a subset of lymphoid cells, in particular by those constituting a GC. However, when introduced into the blood stream, these cells do not home into GC of lymph nodes and gut-associated lymphoid tissues. To investigate further this homing inability for high endothelial venule (HEV)-containing lymphoid tissues, GC cells isolated from donor rabbit appendix were labeled in vitro with 3H-leucine and injected into an afferent lymph vessel of recipient popliteal lymph nodes. Draining lymph nodes were removed 15 min to 24 h after cell administration and prepared for radioautography. For reference, the migration of cells isolated from Peyer's patches and thoracic duct lymph was also studied. By use of appendix GC cells, large numbers of labeled cells were found to migrate into GCs of the outer cortex centripetally, i.e., from the subcapsular sinus through the lymphocyte corona into the GC proper. The same was observed for cells from Peyer's patches, although in smaller numbers. Thoracic duct lymphocytes were only localized in the lymphocyte corona and the deep cortex. Thus, appendix GC cells and a subpopulation of cells from Peyer's patches can reach lymph node GC, but only when administered intralymphatically. We conclude that cells expressing affinity for the GC microenvironment do so for both spleen and lymph node GC, but do not have the capacity to interact with the wall of HEV; its implication for the understanding of the dynamics of a GC reaction is discussed.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 16
    Digitale Medien
    Digitale Medien
    Springer
    Cell & tissue research 238 (1984), S. 489-496 
    ISSN: 1432-0878
    Schlagwort(e): Vitamin D ; Skin ; Autoradiography ; Hair ; Development
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Tritium-labeled 1,25 (OH2) vitamin D3, when injected into vitamin D-deficient adult and pregnant rats is concentrated and retained strongest in nuclei of cells in the outer root sheath of the hair, followed by the stratum granulosum, spinosum, and basale of the epidermis. In the hair follicle, in addition to the most heavily labeled outer root sheath, nuclear labeling exists also in cells of the hair bulb and of the inner root sheath, as well as in basal cells of the sebaceous gland. In contrast, cells of the dermal papilla and the connective tissue of the dermis are generally unlabeled, except for labeled cells in the outer connective tissue sheath at the infundibulum of vibrissae of 20-day fetal rats and a few scattered labeled cells in the dermis, probably macrophages. In the developing hair, in 18- and 20-day fetal rats, a distinct topographic pattern of labeled cells can be seen, which is characteristic of the different stages of hair follicle development. In the hair germ, heavily labeled cells appear first in the stratum spinosum. In the hair peg, they remain in this position in its juxtaepidermal portion; however, when a dermal papilla develops, heavily labeled cells assume a marginal position. This suggests a sequential epidermal-epidermal and mesenchymal-epidermal receptor induction. Injection of tritium labeled 25 (OH) vitamin D3 did not show nuclear concentration in these tissues and excess unlabeled 25 (OH) vitamin D3 — unlike excess 1,25 (OH2) vitamin D3 — did not prevent nuclear uptake of tritium labeled 1,25 (OH2) vitamin D3. The results indicate differential effects of 1,25 (OH2) vitamin D3 on different structures in the epidermis and dermis.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 17
    Digitale Medien
    Digitale Medien
    Springer
    Cell & tissue research 235 (1984), S. 503-508 
    ISSN: 1432-0878
    Schlagwort(e): γ-Aminobutyric acid (GABA) ; Autoradiography ; Pancreas, neonatal rat
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The localization of gamma-aminobutyric acid (GABA) high-affinity binding sites was investigated in the exocrine and endocrine pancreas of neonatal rats by means of 3H-GABA autoradiography. GABA-binding was identified on Schwann cells and on the cells of the intralobular excretory ducts. In the endocrine part of the pancreas, no labelling was observed except in peripheral islet cells which, on the basis of their scarcity and distribution, could be somatostatin cells. Furthermore, peri-insular innervation showed considerable labelling.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 18
    Digitale Medien
    Digitale Medien
    Springer
    Cell & tissue research 237 (1984), S. 387-389 
    ISSN: 1432-0878
    Schlagwort(e): Pituitary gland, pars nervosa ; Autoradiography ; α2Adrenoreceptors ; Clonidine
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Autoradiography of sections from neurohypophyses treated with tritiated clonidine has shown specific binding to α2-adrenoceptor sites in the neurohypophysis but not in the intermediate lobe. Other studies have shown that α2 agonists such as clonidine can cause a fall in circulating antidiuretic hormone; it is therefore possible to speculate that this action could be a direct one on the neurohypophysis since the appropriate binding sites have been shown to exist.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 19
    Digitale Medien
    Digitale Medien
    Springer
    Cell & tissue research 238 (1984), S. 121-128 
    ISSN: 1432-0878
    Schlagwort(e): Ultrastructure ; Autoradiography ; Oocytes ; Nucleolus-like bodies ; Teleost
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Cytoplasmic granulo-fibrillar masses, usually termed nucleolus-like bodies (NLB) or nuages, have been described in several different cell types. They are sometimes associated with a mitochondrial arrangement, this association often being marked during certain phases of the oocyte cycle. In Xiphophorus helleri, NLB consist of fibrillar and granular material that gradually becomes more granular during meiotic prophase I, and is associated with mitochondrial arrangements during diplotene and dictyate of meiosis. Autoradiographic studies of uridine incorporation into the nucleolus and subsequently into NLB suggest that the latter represent a reserve of ribonucleoproteins that is later used in ribosomal maturation during vitellogenesis.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 20
    ISSN: 1432-0878
    Schlagwort(e): Gorgonians ; Organic matrix ; Autoradiography ; Calcification ; Leptogorgia virgulata
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The sequence of the synthesis and transport of the organic matrix of spicules has been elucidated in the gorgonian Leptogorgia virgulata by use of 3H-aspartic acid as the tracer in electron-microscopic autoradiography. The entire process of matrix synthesis and transport takes approximately 2 h. It seems that the protein moiety of the organic matrix is synthesized in the RER prior to 5 min following the initial 10 min incubation in the tracer. At the 5 min chase the label is moving from the RER to the Golgi complexes where the carbohydrate moiety of the matrix is presumed to be synthesized. At the 5 to 15 min chases the label is transported out of the Golgi complexes via Golgi vesicles. This phase continues for 30 min. From 60 to 120 min the 3H-aspartic acid moves to the spicules. After 120 min the majority of the label has moved into the spicules. Silver grain counts over both multivesicular and electron-dense bodies remain at relatively low and constant levels over 4 h indicating that neither organelle is involved in the synthesis and transport of the organic matrix.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 21
    ISSN: 1432-0878
    Schlagwort(e): Pineal gland ; Mammals ; Autoradiography ; [3H]-indoles
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Pineal glands of rat, rabbit and hamster were incubated during day or night in Merlis' fluid containing [3H]-5-hydroxytryptamine (= [3H]-HT) by the use of a 20-min pulse with or without postincubation in “cold” medium for 15, 30, 45 or 60 min. (1) Selective autoradiographic labeling was observed in sympathetic nerve terminals; this reaction was missing after bilateral surgical removal of the superior cervical ganglia. In contrast, a scarce and diffuse labeling was found in pinealocytes (Pi) and interstitial cells (IC) of both untreated and ganglionectomized animals. (2) With the use of thin-layer chromatography, it could be shown in the rat that the well-known indoles of the pineal gland are formed from [3H]-HT. (3) During preparation for electron microscopy (EM), the total loss of indoles from pineal glands was studied by means of liquid-scintillation counting; approximately 57% of the radioactivity of the pineal glands was released into EM-processing solutions, mainly into the glutaraldehyde fixative. In summary, our results show that in this type of experiment with pineal glands of mammals, the routinely used EM-procedures are inadequate to visualize the uptake and metabolism of exogenous indoles in Pi and IC. Furthermore, the data differ considerably from those obtained with the pineal organs of several reptilian and avian species when a similar cytological procedure is used. It appears that protein(s) located in the densely packed vesicles of the pineal cells of sauropsids, homologous to mammalian pinealocytes, may play a crucial role in indole binding (specific indole-binding proteins); this may help to interpret the diverging results obtained in different amniotes.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 22
    Digitale Medien
    Digitale Medien
    Springer
    Cell & tissue research 236 (1984), S. 189-195 
    ISSN: 1432-0878
    Schlagwort(e): Adrenal medulla ; 5-Hydroxytryptamine ; 5-Hydroxytryptophan ; Noradrenaline ; Autoradiography
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Light-microscopic autoradiographs of the adrenal medulla at various intervals after the intravenous injection of [3H] 5-HTP, [3H] 5-HT, [3H] noradrenaline and [3H] adrenaline have been studied. The distribution of silver grains following [3H] 5-HTP uptake was found to be uniform over each of the two main cell populations, adrenaline-storing (A) cells and noradrenaline-storing (NA) cells in the adrenal medulla, but A cells were twice as active as NA cells in incorporating the isotope, a situation very similar to that found after [3H] dopa uptake. 5-HT administration resulted in a pattern resembling the distribution of [3H] noradrenaline uptake, with A cells being 4 or 5 times more active than NA cells and a gradient of activity from the periphery of the medulla inwards. However, the time-course for the loss of radioactivity was not the same for both amines: levels of 5-HT activity were not significantly reduced after one week whereas the degree of [3H] noradrenaline labelling after one week was less than 10% of that at one hour. Thus 5-HT may be bound to sites in the adrenal medulla normally occupied by noradrenaline but it would appear that the release mechanism is different. There was no evidence of 5-HT uptake by adrenal nerve endings.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 23
    Digitale Medien
    Digitale Medien
    Springer
    Cell & tissue research 237 (1984), S. 353-356 
    ISSN: 1432-0878
    Schlagwort(e): Ribosomes, assembly ; Proteins S6, eL12 ; Oocytes ; Autoradiography ; Immunofluorescence ; Xenopus laevis
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The process of ribosome assembly in eukaryotes was studied by injecting tritium-labeled ribosomal proteins S6 and eL12 into oocytes of Xenopus laevis. The subcellular distribution of the two proteins was visualized by means of autoradiography in sections of oocytes. Protein S6 but not eL12 was found in the nucleus where it accumulated at the nucleoli. In the presence of actinomycin D the accumulation of S6 at the nucleoli was reduced. In-situ immunofluorescence studies indicated that S6 is located at the nucleoli and eL12 exclusively in the cytoplasm. It appears that S6 is involved in the early ribosomal assembly process at the nucleoli, whereas eL12 is restricted to the cytoplasm where it is incorporated into 60S ribosomal subunits in a late assembly step.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 24
    ISSN: 1432-0878
    Schlagwort(e): Tertiary follicle (rabbit) ; Ovary ; Ovulation ; Follicle growth ; Vascularisation ; Autoradiography
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The growth of tertiary follicles, i.e., the proliferation of cells in the stratum granulosum and in the capillary network of the theca interna, after injection of ovulation-inducing human chorionic gonadotropin (HCG), was investigated in the rabbit by means of autoradiographic and morphometric methods. Based on the frequency distribution of follicles with different sizes and on the labeling index (LI) of granulosa cells as a function of follicle size and of time prior to and after HCG stimulation, two groups of tertiary tollicles can be distinguished: growing (250–900 μm in diameter) and mature (〉900 μm in diameter) elements. The growth of both groups is influenced by the release of gonadotropins. After HCG stimulation, follicles belonging to the first group grow rapidly. During, and a short time after ovulation, almost all non-ruptured follicles larger than 600 μm in diameter become atretic. Within 35–50 h the ruptured and atretic mature follicles (〉900 μm in diameter) are replaced by follicles out of the group of growing follicles. From these results the following concept for regulation of follicle growth is derived: In principle, all growing follicles possess the potential to develop into mature follicles. When a sufficient number of mature follicles is generated, these mature follicles determine the number of succeeding growing follicles. Follicles that are not required for providing mature follicles become atretic as soon as they reach a diameter of 700 μm. When the majority of mature follicles is lost during ovulation (by rupture or atresia), this inhibition regulated by mature follicles is abolished, and all of the growing follicles again are capable to develop into mature follicles. The relative amount of capillaries in the theca interna of growing and mature follicles remains constant with increasing follicle size. This means that the capillary network grows parallel to the increasing size of follicles. No differences are found between intact and atretic follicles; advanced atretic follicles were excluded from this study. The labeling index (LI) of granulosa cells in the stratum granulosum and of endothelial cells in the theca interna, as a function of follicle size and of time after HCG stimulation, are closely correlated. A change in the LI of granulosa cells is usually followed with a certain delay by a similar alteration of the LI of endothelial cells in the theca interna. This suggests that granulosa cells have a certain regulatory function on capillary growth.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 25
    Digitale Medien
    Digitale Medien
    Springer
    Fresenius' Zeitschrift für analytische Chemie 263 (1973), S. 331-333 
    ISSN: 1618-2650
    Schlagwort(e): Best. von Kohlenmonoxid in Wasserstoff ; Spektralphotometrie ; Ag-Na-Sulfamidbenzoat als Reagens
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 26
    Digitale Medien
    Digitale Medien
    Springer
    Fresenius' Zeitschrift für analytische Chemie 263 (1973), S. 334-334 
    ISSN: 1618-2650
    Schlagwort(e): Best. von Zinn in Zircaloy ; Spektralphotometrie ; Phenylfluoron als Reagens
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 27
    Digitale Medien
    Digitale Medien
    Springer
    Fresenius' Zeitschrift für analytische Chemie 264 (1973), S. 131-133 
    ISSN: 1618-2650
    Schlagwort(e): Best. von Gallium-Organoverbindungen ; Spektralphotometrie ; Extraktion der Dithizonate
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie
    Beschreibung / Inhaltsverzeichnis: Zusammenfassung Die Reaktionsfähigkeit von Organo-Gallium-Verbindungen mit Dithizon wurde festgestellt. Die Dithizonate der alky- loder arylgruppentragenden Pseudoionen RGa2+ und R2Ga+ sind mit violettstichig roter Farbe in Tetra oder Chloroform extrahierbar. Über die Bildung der diesbezüglichen Dithizonate sowie deren charakteristische Eigenschaften werden nähere Angaben gemacht. Die analytische Bestimmung des R2Ga+ ist nach dem photometrischen Mischfarbenverfahren möglich.
    Notizen: Abstract The reactivity of organo-gallium compounds has been established. The dithizonates of the pseudoions RGa2+ and R2Ga+ are in connection with alkyl or aryl groups extractable with red colour with a tinge of violet into carbon tetrachloride or chloroform. Detailed information on the formation of these dithizonates and their characteristic properties is given. The analytical determination of R2Ga+ is possible with the mixed-colour method.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 28
    Digitale Medien
    Digitale Medien
    Springer
    Fresenius' Zeitschrift für analytische Chemie 264 (1973), S. 133-138 
    ISSN: 1618-2650
    Schlagwort(e): Best. von Zinn in Stahl ; Spektralphotometrie ; Brenzcatechinviolett, Phenylfluoron ; Dispergierungsmittel
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie
    Beschreibung / Inhaltsverzeichnis: Summary A survey of existing procedures for the colorimetric determination of tin in steel is given. Recommendations are made with regard to separating tin, masking interfering metals and selecting a colorimetric finish. The effect of dispersing agents on reagents of the type used for determining tin, and on the complexes formed in these determinations, is discussed, including new work on the effect of cetyltrimethylammonium bromide on the phenylfluorone-tin(IV) system.
    Notizen: Zusammenfassung Ein Überblick über colorimetrische Zinnbestimmungsmethoden in Stahl wird gegeben und Vorschläge zur Abtrennung des Zinns, der Maskierung störender Metalle sowie der Auswahl des colorimetrischen Meßverfahrens gemacht. Der Einfluß von Dispergierungsmitteln auf die Eeagentien und gebildeten Komplexe wird diskutiert, wobei über neue Untersuchungen zum Einfluß von Cetyltrimethylammoniumbromid auf das System Phenylfluoron/Zinn(IV) berichtet wird.
    Materialart: Digitale Medien
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  • 29
    Digitale Medien
    Digitale Medien
    Springer
    Fresenius' Zeitschrift für analytische Chemie 264 (1973), S. 412-413 
    ISSN: 1618-2650
    Schlagwort(e): Best. von Phenylquecksilberborat in Augentropfen, Pharmazeut. Produkten ; Spektralphotometrie ; Atomabsorption ; flammenlos
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 30
    Digitale Medien
    Digitale Medien
    Springer
    Fresenius' Zeitschrift für analytische Chemie 265 (1973), S. 29-30 
    ISSN: 1618-2650
    Schlagwort(e): Best. von Antimon in Eisen und Stahl ; Spektralphotometrie ; Methylfluoron als Reagens
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie
    Beschreibung / Inhaltsverzeichnis: Zusammenfassung Zur Bestimmung von 2,5–100 ppm Sb in Eisen, unlegierten und niedrig legierten Stählen sowie in Automatenstählen wird das Antimon zuerst in 2 Stufen vorisoliert mit Hilfe der Spurenfällung mit Mangan(IV)-oxidhydrat als Spurenfänger und anschließender Jodidextraktion. Nach der Rückextraktion erfolgt die photometrische Bestimmung mit Methylfluoron in der wäßrigen Phase. Der relative Fehler beträgt ±5%, der Zeitbedarf für eine Einzelbestimmung 1,5 h.
    Notizen: Abstract For the determination of 2.5–100 ppm Sb in iron, plain carbon steels, low-alloy and free-cutting steels antimony is at first separated in two steps. It is coprecipitated with manganese(IV)oxide hydrate as collector, the precipitate dissolved and antimony extracted as iodide with benzene. After back extraction antimony is determined photometrically in the aqueous phase with methylfluorone. The relative error is ± 5%. One determination requires 1.5 h.
    Materialart: Digitale Medien
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  • 31
    Digitale Medien
    Digitale Medien
    Springer
    Fresenius' Zeitschrift für analytische Chemie 265 (1973), S. 104-113 
    ISSN: 1618-2650
    Schlagwort(e): Spektralphotometrie ; Spurenanalyse ; Sensibilisierung eines Zweizellen-Photocolorimeters
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie
    Beschreibung / Inhaltsverzeichnis: Zusammenfassung Eine spektralphotometrische Methode wird beschrieben, die es ermöglicht, Elemente in kleinen Konzentrationen zu bestimmen. Dies wird im Prinzip durch den Anschluß eines hochempfindlichen Galvanometers an ein Zweizellenphotocolorimeter ermöglicht. Der so erreichte Gewinn an Empfindlichkeit wird einesteils durch die Anwendung von zwei Interferenzfiltern dazu genutzt, die Selektivität des Systems zu erhöhen, anderenteils kann mit dem Ersatz der Lichtschutzklappe durch Küvetten mit sog. Limitlösungen (Lösungen bekannter, entsprechender Konzentration) das System auf den optimalen Meßbereich ausgewogen werden. Zu den Hauptvorteilen dieser Methode zählen: Senkung der Nachweisgrenze (z.B. bei Mn von 20,27 auf 0,18 μg), variable Wahl der optimalen Empfindlichkeit, optimales Auflösungsvermögen durch die Möglichkeit der Skalendehnung im gewünschten Konzentrationsintervall, Erhöhung der Genauigkeit sowie leichte Konstruktion der diagonal zum Koordinatenkreuz unter einem Winkel von 45° verlaufenden Eichkurve.
    Notizen: Abstract A spectrophotometric method is described by which it is possible to determine elements in low concentrations. This is achieved by the connection of a highly sensitive galvanometer to a two-cell photocolorimeter. The higher precision attained in this way can be used on the one hand by the application of two interference filters for the increase of the selectivity of the system, on the other hand, with the replacement of the flap by measurement cells with so-called limit solutions (solutions of known, corresponding concentration) for balancing the system for an optimum range of measurement. The main advantages of this method are: decrease of the limit of detection (e.g. of Mn from 20.27 to 0.18 μg), choice of an optimum sensitivity, an optimum separating power by the possibility of extending the scale in the necessary concentration interval, increase of precision and an easy construction of the calibration curve, located diagonally under a 45° angle to the coordinate system.
    Materialart: Digitale Medien
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  • 32
    Digitale Medien
    Digitale Medien
    Springer
    Fresenius' Zeitschrift für analytische Chemie 265 (1973), S. 260-266 
    ISSN: 1618-2650
    Schlagwort(e): Best. von Zirkonium mit 2,3,7-Trihydroxy-9-(2-chinolyl)-isoxanthenon-(6), 2′-Chinolylfluoron ; Spektralphotometrie
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie
    Beschreibung / Inhaltsverzeichnis: Zusammenfassung Die Darstellung des 2′-Chinolylfluorons und dessen Eigenschaften werden beschrieben. Es wird gezeigt, daß das Reagens im sauren Medium mit Zirkonium mehrere Komplexe bildet, darunter einen mit der Zusammensetzung 1∶4. Dieser bevorzugt bei Fluoronüberschuß gebildete Komplex besitzt den extrem hohen Extinktionskoeffizienten ɛ max=165000 l/Mol · cm und eignet sich vorzüglich zur Ausarbeitung eines photometrischen Bestimmungsverfahrens für Zirkonium. Im Konzentrationsbereich 0,4–10 nMol/ml liegen die Variationskoeffizienten des Verfahrens zwischen 0,5 und 2,8 Rel-%.
    Notizen: Abstract A report is given on the preparation and properties of 2′-quinolylfluorone. In acid solution the reagent forms various complexes with zirconium. Owing to its extremely high extinction coefficient ɛ max=165000 the 1∶4-complex can be used for quantitative determination of zirconium. In the concentration range 0.4–10 nmoles/ml the variation coefficients are 0.5–2.8% rel.
    Materialart: Digitale Medien
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  • 33
    Digitale Medien
    Digitale Medien
    Springer
    Fresenius' Zeitschrift für analytische Chemie 265 (1973), S. 267-268 
    ISSN: 1618-2650
    Schlagwort(e): Best. von Zirkoniumoxidchlorid in Natriumchlorid ; Spektralphotometrie ; Speisesalzanalyse
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie
    Beschreibung / Inhaltsverzeichnis: Zusammenfassung 2′-Chinolylfluoron bildet mit Zirkonium einen 1∶4-Komplex, dessen Extinktionskoeffizient bei 539 nm ɛ max=165000 ist. Das Bestimmungsverfahren für ZrOCl2 in Speisesalz wird auf dieser Farbreaktion aufgebaut.
    Notizen: Abstract 2′-Quinolylfluorone forms a 1∶4-complex with zirconium. Owing to its high extinction coefficient ɛ max=165000 at λ=539 nm the complex can be used for the determination of ZrOCl2 in table salt.
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  • 34
    Digitale Medien
    Digitale Medien
    Springer
    Fresenius' Zeitschrift für analytische Chemie 265 (1973), S. 269-269 
    ISSN: 1618-2650
    Schlagwort(e): Bildungskonstanten des Komplexes Eisen(II)/Chinolinsäure ; Spektralphotometrie
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 35
    Digitale Medien
    Digitale Medien
    Springer
    Fresenius' Zeitschrift für analytische Chemie 265 (1973), S. 272-272 
    ISSN: 1618-2650
    Schlagwort(e): Best. von Phenol-2, 4-disulfonsäure neben Phenol-4-sulfonsäure ; Spektralphotometrie ; Eisen(III)-komplex
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 36
    Digitale Medien
    Digitale Medien
    Springer
    Fresenius' Zeitschrift für analytische Chemie 266 (1973), S. 343-348 
    ISSN: 1618-2650
    Schlagwort(e): Best. von Magnesium in Aluminiumlegierungen ; Spektralphotometrie ; DHBTM als Reagens
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie
    Beschreibung / Inhaltsverzeichnis: Zusammenfassung Durch Kondensation von Malonaldehyd mit 4,6-Dioxohexahydropyrimidin (Dihydrobarbitursäure) bildet sich der Trimethinfarbstoff DHBTM. Er ist ein ausgezeichnetes, stabiles Reagens zur photometrischen Bestimmung von Magnesium. Seine Darstellung und seine Eigenschaften werden beschrieben. Die Magnesiumbestimmung beruht auf der Bildung einer Adsorptionsverbindung und ist weitgehend unabhängig von Störfaktoren, die bei ähnlichen Reaktionen von großem Einfluß sind. So läßt sich ohne voraufgehende Extraktion, lediglich unter Zuhilfenahme von Maskierungsmitteln ein Bestimmungsverfahren für Magnesium in Aluminramlegierungen ausarbeiten. Der Variationskoeffizient des Verfahrens liegt in der Größenordnung von 1 Rel.-%.
    Notizen: Abstract The trimethine dye DHBTM, which can be prepared by condensation of malonaldehyde and 4,6-dioxohexahydropyrimidine (dihydrobarbituric acid), is described as an excellent, stable reagent for a photometric determination of magnesium. The method is based on the formation of an adsorption compound. Interfering elements in aluminium alloys can be masked in a simple manner with no preceding extraction being necessary. The variation coefficient of the photometric method is ca. 1%-rel.
    Materialart: Digitale Medien
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  • 37
    Digitale Medien
    Digitale Medien
    Springer
    Fresenius' Zeitschrift für analytische Chemie 266 (1973), S. 348-351 
    ISSN: 1618-2650
    Schlagwort(e): Best. von Ammoniumhydroxid in Ammoniumfluorid-Pufferlösungen ; Spektralphotometrie ; Indicatoren der Phenolgruppe
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie
    Beschreibung / Inhaltsverzeichnis: Zusammenfassung Zur reproduzierbaren Herstellung von NH4F-HF-Ätzlösungen muß der Gehalt an NH4OH in den käuflichen 40% igen NH4F-Lösungen ermittelt werden. Hierzu wurde eine colorimetrische Methode unter Verwendung von pH-Farbindicatoren der Phenolgruppe ausgearbeitet, die geringe pH-Wertabweichungen (0,007 pH) vom Soll (0% NH4OH) zu messen erlaubt, wobei der Sollwert durch stabile Citronensäure-Phosphatpuffer simuliert wird. Durch Extinktionsmessung gelingt es, 0,01% NH4OH in NH4F-Lösungen zu erfassen. Die gefundenen Werte liegen zwischen 0,1–0,3%.
    Notizen: Abstract To obtain NH4F-HF etching agents with constant etching behaviour the concentration of NH4OH in commercially available 40% NH4F solutions has to be measured. By application of pH-indicators of the phenol type very small pH-deviations (0.007 pH) from the specified value (0% NH4OH) can be measured. The specified value is simulated by the stable McIlvaine buffer. By determining the differences in optical absorption 0.01% NH4OH can be measured in NH4F solutions. Values found in practice are between 0.1– 0.3%.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 38
    Digitale Medien
    Digitale Medien
    Springer
    Fresenius' Zeitschrift für analytische Chemie 266 (1973), S. 364-365 
    ISSN: 1618-2650
    Schlagwort(e): Best. von Titan(IV) mit Pimelyl-bis-N-hydroxamsäure ; Spektralphotometrie ; Synergist, m. NH4SCN
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 39
    Digitale Medien
    Digitale Medien
    Springer
    Fresenius' Zeitschrift für analytische Chemie 267 (1973), S. 13-16 
    ISSN: 1618-2650
    Schlagwort(e): Best. von Aluminium in Eisen, Stahl ; Spektralphotometrie ; Aluminonmethode nach Extraktionen und Elektrolyse
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie
    Beschreibung / Inhaltsverzeichnis: Zusammenfassung Das beschriebene photometrische Verfahren wurde für Al-Gehalte von 0,01–1,1% in Eisen, Stählen und Edelstählen ausgearbeitet und an Teststählen überprüft. Mit Hilfe einer Hg-Elektrolyse und schrittweise vorgenommenen Extraktionen der die Al-Bestimmung störenden Legierungsbestandteile (wie Fe, Mo, Cr, Cu, Co, Cd, Mo, Pb, Ni, Sb, Zn, Sn, Mn) wird das Aluminium mit 8-Hydroxychinolin bei pH 9 abgetrennt und nach einem HNO3/H2SO4-Aufschluß photometrisch nach der Aluminonmethode bestimmt.
    Notizen: Abstract The method has been worked out for Al contents from 0.01 to 1.1% in iron, steels and alloy steels, and tested with standard reference materials. Aluminium is separated by 8-hydroxy-quinoline at pH 9 after Hg-electrolysis and step-wise pre-extraction of interfering constituents as Fe, Mo, Cr, Cu, Co, Cd, Mo, Pb, Ni, Sb, Zn, Sn, Mn. Finally the 8-hydroxy-quinoline extract is digested with nitric/sulphuric acid and aluminium is determined spectrophotometrically by the Aluminon method.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 40
    Digitale Medien
    Digitale Medien
    Springer
    Fresenius' Zeitschrift für analytische Chemie 267 (1973), S. 44-44 
    ISSN: 1618-2650
    Schlagwort(e): Best. von Carbonylverbindungen mit 2,4-Dinitrophenylhydrazin ; Spektralphotometrie
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 41
    Digitale Medien
    Digitale Medien
    Springer
    Fresenius' Zeitschrift für analytische Chemie 267 (1973), S. 89-95 
    ISSN: 1618-2650
    Schlagwort(e): Best. von Jod in Pflanzenmaterial, Filterpapier ; Spektralphotometrie ; ng-Bereich, Sandell-Kolthoff-Reaktion
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie
    Beschreibung / Inhaltsverzeichnis: Zusammenfassung Bei einem Verfahren zur Jodbestimmung in Biomatrices auf Grund der Sandell-Kolthoff-Reaktion wurden der alkalische Aufschluß sowie weitere Verfahrensschritte mit 131J-Tracer auf Jodverlust und Reproduzierbarkeit überprüft und eine modifizierte Arbeitsvorschrift ausgearbeitet. Bei Grasproben wurde eine Standardabweichung von 7% ermittelt.
    Notizen: Abstract A method for the determination of iodine in biological materials based on the Sandell-Kolthoff reaction has been studied. For the alkaline mineralization and some further steps with regard to iodine losses and reproducibility 131I tracer was applied and a modified procedure has been developed. In the case of grass samples the standard deviation is found to be 7%.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 42
    Digitale Medien
    Digitale Medien
    Springer
    Fresenius' Zeitschrift für analytische Chemie 267 (1973), S. 103-105 
    ISSN: 1618-2650
    Schlagwort(e): Best. des O/U-Verhältnisses von nichtstöchiometr ; Urandioxid ; Spektralphotometrie
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie
    Beschreibung / Inhaltsverzeichnis: Zusammenfassung Eine Methode zur Bestimmung des Sauerstoff-Metall-Verhältnisses von überstöchiometrischem Urandioxid wird beschrieben. Nach Auflösen des Urandioxids in heißer konzentrierter Phosphorsäure werden die Konzentrationen an U4+ und U6+ über die Messung der Extinktionen zweier spezifischer Wellenlängen bestimmt. Die Methode zeichnet sich durch Einfachheit und geringen Zeitaufwand aus. Sie wurde für O/U-Verhältnisse von 2,05–2,67 getestet. Die Reproduzierbarkeit beträgt, bezogen auf das O/U-Verhältnis, ±0,005.
    Notizen: Abstract After dissolution of the sample in hot concentrated phosphoric acid the concentrations of U4+ and U6+ are measured via the absorbance of two specific wavelengths. The method is simple and requires little time. It has been tested for 0/U ratios 2.05 to 2.67. The reproducibility, related to the O/U ratio, is ± 0.005.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 43
    Digitale Medien
    Digitale Medien
    Springer
    Fresenius' Zeitschrift für analytische Chemie 267 (1973), S. 127-128 
    ISSN: 1618-2650
    Schlagwort(e): Best. von Phthalsäureestern in Polyvinylchlorid, Weichmachern ; Chromatographie, Dünnschicht ; Spektralphotometrie
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie
    Beschreibung / Inhaltsverzeichnis: Zusammenfassung Eine dünnschicht-chromatographische Methode zur quantitativen Bestimmung von Phthalsäureestern in wäßrigen Weich-PVC-Extrakten wurde entwickelt. Als Lösungsmittel dient Petroläther-Diäthyläther-Eisessig (8∶2∶0,1); die Beschichtung besteht aus 0,2 mm Silicagel mit Fluorescenzzusatz. Nach Auftrennung und Elution erfolgt eine photometrische Auswertung.
    Notizen: Abstract A thinlayer chromatographic method for the quantitative determination of phthalic acid esters in aqueous PVC-plastic extracts has been developed. Petroleum ether/diethyl ether/glacial acetic acid (8∶2∶0.1) is used as solvent; the plates are coated with 0.2 mm of silica gel with fluorescent agent. After separation and elution a photometric determination is performed.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 44
    Digitale Medien
    Digitale Medien
    Springer
    Fresenius' Zeitschrift für analytische Chemie 267 (1973), S. 288-288 
    ISSN: 1618-2650
    Schlagwort(e): Best. von Platin(IV) ; Spektralphotometrie ; UV-Bereich
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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