ALBERT

Notes: The equilibrium thermodynamics of the reaction:And the equilibrium constant is composed of activities formulated using ideal mixing on sites. Consideration is given to the evaluation of uncertainties in pressures calculated using the geobarometer. Preliminary testing suggests that the geobarometer has considerable potential. Much wider testing is now required.

Notes: Abstract. Pink piemontite-spessartine-bearing and grey-green spessartine-bearing manganiferous quartzose schists derived from siliceous pelagites, and green quartzofeldspathic schists, are described from the greenschist facies of the Haast Schist terrane, near Arrow Junction, western Otago. Electron microprobe data are reported for sphene, spessartine-rich garnet, manganoan epidote, piemontite, tourmaline, phengitic muscovite, chlorite, albite, haematite, rutile, manganoan calcite and chalcopyrite.Metamorphism occurred at about 6.4kbar, 400°C. Xco2 was above the quartz-rutile-calcite-sphene buffer (Xco2± 0.02) throughout the recorded metamorphic history of the piemontite schists. It dropped from above to below this critical buffering value in a spessartine-rich schist and it was close to or below the buffering value in the quartzofeldspathic schists. Production of piemontite required high fO2, believed to be inherited from MnOx in the parent pelagite. Substantial loss of O2 (e.g. minimum of 0.19% by weight in one rock) during diagenesis and/or metamorphism is inferred. In the grey-green schists this inhibited piemontite formation. Slight loss of O2 and Ca2+ accompanied minor late-stage replacement of piemontite by second generation spessartine. Observed zoning and mineral replacements indicate rise of temperature, drop in pressure, or invasion by solutions of lower fO2 and XCO2 equilibrated with surrounding schists.The detailed chemistry of the minerals studied correlates with available Mn and with bulk-rock (Fe3+ x 100)/(Fe2++ Fe3+). The oxidation ratio ranges from 24 in average green quartzofeldspathic schist, through 78 in average grey-green manganiferous quartzose schist, to almost 100 in some piemontite-bearing schists. As Fe2+ gives way to Fe3+, Mg/Fe ratios tend to rise in chlorite, phengite, tourmaline, spessartine, and calcite, Mn increases and Ti decreases in haematite, Mn increases in spessartine and calcite, and Fe increases in rutile. Available divalent cations are depleted relative to Al; chlorite is more aluminous, and phengite more paragonitic than in typical Haast schists.

Notes: Field, petrographic and microprobe investigations of metaclastic rocks, calcareous schists, marbles, chloritic calcareous meta-volcanic units and schists/paragneisses which crop out along the eastern portion of the Central East-West Cross Island Highway in Taiwan demonstrate that metamorphic intensity gradually increases eastward. The lower greenschist facies Slate Formation on the W contains completely recrystallized, pure albitic plagioclase, but at least some of the white micas (± chlorites) probably represent relict detrital flakes. Neo-blastic biotite and epidote occur sporadically in the Pihou(?) Formation, and increase dramatically eastward; concomitantly the abundance of carbonaceous matter decreases to zero in the eastern Tailuko zone, and the amount of chlorite + white mica diminishes somewhat. Epidote becomes more aluminous at higher metamorphic grade. Eastward, phengites change progressively to more muscovitic compositions as the proportion of biotite increases.A close approach to chemical equilibrium for the pre-Cenozoic, complexly deformed metamorphic basement assemblages is suggested by regular, systematic, major and minor element partitioning between analysed coexisting phases. Fractionation is less pronounced on the E, reflecting higher temperatures. Estimated physical conditions of recrystallization with αH2O and αCO2 moderate, are: T 〉 325 ± 75°C, P 〉 3 kbar (W); T 〉 425 ± 75°C, P 〉 4kbar(E).The gradual eastward increase in metamorphic intensity from the Slate Formation through the Pihou(?) Formation and the three Tailuko zones, as well as the relict precursor textures in the pre-Cenozoic layered basement rocks indicate that the observed paragenetic sequence could represent a synchronous Neogene recrystallization event, probably accompanying the Plio-Pleistocene collision of the Asiatic continental margin and the Luzon (Coastal Range) andesitic arc.

Notes: The structure, microstructure and petrology of a small area close to the village of Bard in Val d'Aosta (Italy) has been studied in detail. The area lies across the contact between the Gneiss Minuti (GM) and the Eclogitic Micaschist (EMS) Complexes of the Lower element of the Sesia portion of the Sesia-Lanzo Zone (Western Alps). Both complexes have undergone high-pressure metamorphism, but the metamorphic assemblages indicate a sudden increase in pressure in going across the contact from the GM to the EMS. Therefore, we interpret the contact as a thrust dividing the lower element of the Sesia into two sub-elements. This interpretation is supported by structural evidence.The early Alpine (90-70 Ma) metamorphic history is best preserved in the EMS and is one of increasing pressure associated with thrusting. The maximum P/T recorded in the EMS is 〉1500 MPa (〉15kbar) and 550°C and in the GM is 〈 1500-1300 MPa (〈 15-13 kbar) and 500-550°C. We suggest that the rocks were probably in an active Benioff zone during this time.From then on the histories of the GM and EMS are the same. Deformation continued and the thrust and thrust slices were folded during decreasing pressure. We interpret the first postthrusting deformation in terms of uplift associated with continued shortening of the crust and underplating after the Benioff zone had become inactive and a new Benioff zone had developed further to the north-west.A still later deformation and the Lepontine metamorphism (38 Ma) are related to continued uplift. Much of this deformation is characterized by structures indicative of vertical shortening and lateral spreading as the mountains rose above the general level of the surface.

Notes: In the Boi Massif of Western Timor the Mutis Complex, which is equivalent to the Lolotoi Complex of East Timor, is composed of two lithostratigraphical components: various basement schists and gneisses; and the dismembered remnants of an ophiolite. Cordierite-bearing pelitic schists and gneisses carry an early mineral assemblage of biotite + garnet + plagioclase + Al-silicate, but contain no prograde muscovite; sillimanite occurs in a textural mode which suggests that it replaced and pseudomorphed kyanite at an early stage and some specimens of pelitic schist contain tiny kyanite relics in plagioclase. Textural relations between, and mineral chemistries of, ferro-magnesian phases in these pelitic chists and gneisses suggest that two discontinuous reactions and additional continuous compositional changes have been overstepped, possibly with concomitant anatexis, as a result of decrease in Pload during high temperature metamorphism. The simplified reactions are: garnet and/or biotite + sillimanite + quartz + cordierite + hercynite + ilmenite + excess components. P-T conditions during the development of the early mineral assemblage in the pelitic gneisses are estimated to have been P + 10 kbar and T 〉 750°C, based upon the plagioclase-garnet-Al-silicate-quartz geobarometer and the garnet-biotite geothermometer. P-T conditions during the subsequent development of cordierite-bearing mineral assemblages in the pelitic gneisses are estimated to have been P + 5 kbar and T + 700°C with XH2O 〈 0.5, based upon the Fe content of cordierite occurring in the assemblage quartz + plagioclase + sillimanite + biotite + garnet + cordierite coexisting with melt.Final equilibration between some of the phases suggests that conditions dropped to P 〉 2.3 kbar and T 〉 600°C. A similar exhumation P-T path is suggested for the pelitic schists with early metamorphic conditions of P 〉 6.2 kbar and T 〉 745°C and subsequent development of cordierite under conditions in the range P = 3-4 kbar and T = 600-700°C. The tectonic implications of these P-T estimates are discussed and it is concluded that the P-T path followed by these rocks was caused by decompression during rifting and synmetamorphic ophiolite emplacement resulting from processes during the initiation and development of a convergent plate junction located in Southeast Asia during late Jurassic to Cretaceous time.

Notes: Plagioclase compositions vary from An0.1–2.5 to An32 with increasing grade in chlorite zone to oligoclase zone quartzofeldspathic schists, Franz Josef-Fox Glacier area, Southern Alps, New Zealand. This change is interrupted by the peristerite composition gap in rocks transitional between greenschist and amphibolite facies grade. Oligoclase (An20-24) and albite (An0.1–0.5) are found in biotite zone schists below the garnet isograd. With increasing grade, the plagioclase compositions outline the peristerite gap, which is asymmetric and narrows to compositions of An12 and An6 near the top of the garnet zone. In any one sample, oligoclase is the stable mineral in mica-rich layers above the garnet isograd, whereas albite and oligoclase exist in apparent textural equilibrium in adjacent quartz-plagioclase layers. The initial appearance of oligoclase in both layers results from the breakdown of epidote and possibly sphene. Carbonate is restricted to the quartz-plagioclase rich layers and probably accounts for the more sodic composition of oligoclase in these layers. The formation of more Ca-rich albite and more Na-rich oligoclase near the upper limit of the garnet zone coincides with the disappearance of carbonate and closure of the peristerite gap. Garnet appears to have only a localized effect on Ca-enrichment of plagioclase in mica-rich layers within the garnet zone. The Na-content of white mica increases sympathetically with increasing Ca-content of oligoclase and metamorphic grade.Comparison of the peristerite gap in the Franz Josef-Fox Glacier schists and schists of the same bulk composition in the Haast River area, 80 km to the S, indicates that oligoclase appears and epidote disappears at lower temperatures, and that the composition gap between coexisting albite and oligoclase is narrower in the Franz Josef-Fox Glacier area. It is suggested that a higher thermal gradient (38-40°C/km) and variations in Si/Al ordering during growth of the plagioclases between the two areas may account for these differences. In the Alpine schists the peristerite gap exists over a temperature and pressure interval of about 370-515°C and 5.5-7 kbar (550-700 MPa) PH2O.

Notes: Sapphirine occurs in a 3-5 m wide zone between amphibole-lherzolite and garnetiferous metagabbro at Finero in the Ivrea Zone, NW Italian Alps. Layers consisting of plag + hb + sa + cpx + opx + sp + gt are interbanded with spinel pyroxenites, which may contain sapphirine replacing spinel. All minerals are very magnesian, with XMg between 0.78 and 0.92. Bulk rock analyses suggest that precursors to the sapphirine-bearing rocks were igneous cumulates of plagioclase + olivine + hornblende + spinel. Up to 16wt% CaO does not inhibit sapphirine formation and it is the unusually Mg-rich nature of the host rocks which allows sapphirine development. The early igneous assemblage was replaced by one of cpx + sa + hb +± plag at a pressure of 9 ± 1 kbar and temperatures of 900 ± 50°C. Subsequent rapid uplift caused the instability of gt, gt + hb, hb and sa + cpx to form opx + plag ± sp ± sa symplectites.

Notes: The Gran Paradiso basement complex of the French and Italian Alps is composed of metasediments, termed the gneiss minuti, and metabasic rocks, both of which are intruded by a late Hercynian granite. The Bonneval gneiss, which crops out at the western edge of the complex, is composed of highly deformed metasediments, volcanics and volcaniclastic rocks. Eclogites, now highly altered, occur in the metabasic rocks. Kyanite and blue-green amphibole are locally present in the gneiss minuti and aegirine plus riebeckite occur in the Bonneval gneiss. A moderately high pressure - low temperature metamorphic event of probable Alpine age occurred in the basement complex. This metamorphic event differs from that in the overlying Sesia unit and ophiolites of the Schistes lustrés nappe in being at lower pressures (below the ab = jd100+ qz transition) and post-dating the major (D2A) deformation. The origin of the metamorphism is discussed and interpreted as a probable consequence of the overlying nappe pile which was emplaced during the D2A event. Subsequent greenschist facies metamorphism in the basement complex is a consequence of thermal relaxation during uplift.

Notes: Abstract. A method for the quantitative analysis of the spatial relations of minerals is described. Dispersed distributions are formed by annealing and destroyed in post-tectonic migmatization. Aggregate distributions characterize solid-state differentiation, whereas leucosomes formed in systems of high fluid:rock ratio (in the examples studied, anatectic melts) show random distributions.Quantitative textural analysis can be used to indicate whether migmatization was post-tectonic or earlier, though caution is necessary if post-migmatite cooling is slow or if there is some minor deformation. More importantly, it can be used to discriminate melt-present from melt-absent leucosomes; this is exemplified by a suite of metamorphic and anatectic migmatites from the Scottish Caledonides.The textural evolution of anatexites with increasing melt percentage is traced. Initial feldspar porphyroblastesis occurs by Ostwald ripening via grain boundary melts; subsequently ophthalmites develop with fabrics and chemistry inherited from the palaeosome. At greater than 30% melt these inherited fabrics are wholly destroyed. Deformation prompts segregation into melanosome and leucosome; resultant leucosomes contain no inherited crystals. The scale of anatectic systems is fixed at the point at which segregation begins; ophthalmites provide evidence for melt and crystal transfer beyond original palaeosome boundaries. In contrast, metamorphic migmatites are necessarily small-scale systems because of diffusive constraints, and melanosomes are invariably produced.

Notes: The oligoclase-biotite zone of the Bessi area, central Shikoku is characterized by sodic plagioclase (XCa= 0.10–0.28)-bearing assemblages in pelitic schists, and represents the highest-grade zone of the Sanbagawa metamorphic terrain. Mineral assemblages in pelitic schists of this zone, all with quartz, sodic plagioclase, muscovite and clinozoisite (or zoisite), are garnet + biotite + chlorite + paragonite, garnet + biotite + hornblende + chlorite, and partial assemblages of these two types. Correlations between mineral compositions, mineral assemblages and mineral stability data assuming PH2O = Psolid suggests that metamorphic conditions of this zone are about 610 ± 25°C and 10 ± 1 kbar.Based upon a comparative study of mineralogy and chemistry of pelitic schists in the oligoclase-biotite zone of the Sanbagawa terrain with those in the New Caledonia omphacite zone as an example of a typical high-pressure type of metamorphic belt and with those in a generalized‘upper staurolite zone’as an example of a medium-pressure type of metamorphic belt, progressive assemblages within these three zones can be related by reactions such as:

Notes: An assemblage consisting of corundum, sapphirine, spinel, cordierite, garnet, biotite and bronzite is described from the Messina area of the Limpopo Mobile Belt, and consideration given to its petrogenesis. Various geothermometers and geobarometers have been applied in an attempt to determine the temperatures and pressures of metamorphism.A former coexistence of garnet and corundum is suggested to have developed during the earliest high pressure phase of the metamorphism, where temperatures exceeded 800°C and pressures as high as 10kbar may have been experienced. Subsequently, continuous retrograding reactions from medium pressure granulite facies at about 800°C and 8kbar towards amphibolite facies generated spinel, cordierite, sapphirine and possibly also bronzite. The most notable reaction was probably of the form: garnet + corundum = cordierite + sapphirine + spinel.

Notes: A review of currently available information relevant to the Basal Gneiss Complex (BGC) of Western South Norway, combined with the authors’own observations, leads to the following conclusions.1. Most of the BGC consists of Proterozoic crystalline rocks and probably subordinate Lower Palaeozoic cover.2. The last major deformation of these rocks was during the Caledonian orogeny and involved large-scale thrusting, recumbent folding and doming. The structural development of the BGC is closely tied in with that of the Caledonian allochthon.3. The whole eclogite-bearing part of the BGC has suffered a high pressure metamorphism with conditions of between 550°C, 12.5 kbar (Sunnfjord) and about 750°C, 20 kbar (Møre og Romsdal) at the metamorphic climax.4. This metamorphism was of Caledonian age, probably rather early in the Caledonian tectonic history of the BGC and is considered to have been a rather transient event.By setting these conclusions in a framework provided by geophysical evidence for the deep structure of the crust in southern Norway we have constructed a geotectonic model to explain the recorded metamorphic history of the BGC. It is suggested that considerable crustal thickening was caused by imbrication of the Baltic plate margin during continental collision with the Greenland plate. This resulted in high pressure metamorphism in the resulting nappe stack. Progradation of the suture caused underthrusting of the Baltic foreland below the eclogite-bearing terrain causing it to emerge at the Earth's surface, aided by tectonic stripping and erosion.Application of isostacy equations to the model shows that eclogites can be formed by in-situ metamorphism in crustal rocks and reappear at the land surface above a normal thickness of crust in a single orogenic episode of approximately 65-70 Ma duration.

Notes: Detailed geochronological, structural and petrological studies reveal that the geological evolution of the Field Islands area, East Antarctica, was substantially similar to that of the adjacent Archaean Napier Complex, though with notable differences in late and post Archaean times. These differences reflect the area's proximity to the Proterozoic Rayner Complex and consequent vulnerability to tectonic process involved in the formation of the latter. Distinctive structural features of the Field Islands are (1) consistent development of a discordant, pervasive S3 axial-plane foliation; (2) re-orientation of S3 axial planes to approximate to the subsequent E-W tectonic trend of the nearby Rayner Complex; (3) selective retrogression by a post-D3 static thermal overprint; and (4) relatively common development of retrogressive, E-W-trending, mylonitic shear zones.Peak metamorphic conditions in excess of 800°C at 900 ± 100 M Pa (9 kbar) were attained at one locality following, but probably close to the time of D2 folding. D3 took place in late Archaean times when metamorphic temperatures were about 650°C and pressures were about 600 MPa (6 kbar). Later, temperatures of 600 ± 50°C and pressures of 700 MPa (7kbar) were attained in an amphibolite-facies event, presumably associated with the widespread granulite to amphibolite-facies metamorphism and intense deformation involved in the formation of the Rayner Complex at about 1100 Ma. The area was subsequently subjected to near-isothermal uplift.Rb-Sr isotopic data indicate that the pervasive D3 fabric developed at about 2400–2500 Ma, and this age can be further refined to 2456+8-5 Ma by concordant zircon analyses from a syn-D3 pegmatite. All zircons were affected by only minor (〈7–10%) Pb loss and/or new zircon growth during the Rayner event at about 1100Ma. Thus the 450–850 μg/gU concentrations of these zircons were too low to cause sufficient lattice damage over the 1350 Ma (from 2450 Ma) for excessive Pb to be lost during the 1100 Ma event. The emplacement of pegmatite at 522 ± 10 Ma substantially changed the Rb-Sr systematics of the only analysed rock that developed a penetrative fabric during the 1100 Ma event. Monazite in this pegmatite contains an inherited Pb component, which probably resides in small opaque inclusions.A good correlation is found between Rb-Sr total-rock ages and rock fabric. U-Pb zircon intercepts with concordia also mostly correspond to known events. However, in one example a near perfect alignment of zircon analyses, probably developed by mixing of unrelated components, produced concordia intercepts that appear to have no direct geochronological significance.

Notes: An occurrence of quartz-eclogite is described from the Inner Schieferhülle unit of the Pennine Basement Complex in the SE Tauern Window, Austria.Field relations strongly suggest a pre-Alpine age for the primary eclogitic mineral assemblage (garnet + omphacite + quartz + rutile). This implies that there was no connection between the formation of these eclogites and the late Cretaceous and Tertiary tectonic evolution of the Eastern Alps. The quartz-eclogite mineral assemblage crystallized under conditions of 620 ± 100°C and at pressures in excess of 12 kbar, and suffered amphibolitic overprinting of Alpine and possibly Hercynian age.A four-stage polymetamorphic history is proposed for the Inner Schieferhülle:

Notes: Mafic and ultramafic xenoliths in a basaltic cone at The Anakies in south-eastern Australia are geochemically equivalent to continental basaltic magmas and cumulates. The xenolith microstructures range from recognizably meta-igneous for intrusive rocks to granoblastic for garnet pyroxenites. Contact relationships between different rock types within some xenoliths suggest a complex petrogenesis of multiple intrusive, metamorphic and metasomatic events at the crust/mantle boundary during the evolution of south-eastern Australia. Unaltered spinel lher-zolite, typical of the uppermost eastern Australian mantle, is interleaved with or veined by the metamorphosed intrusive rocks of basaltic composition.Geothermobarometry calculations by a variety of methods show a concordance of equilibration temperatures ranging from 880°C to 980°C and pressures of 12 to 18 kbar (1200-1800 mPa). These physical conditions span the gabbro to granulite to eclogite transition boundaries. The water-vapour pressure during equilibration is estimated to be about 0.5% of the load pressure, using amphibole breakdown data. Large fluid inclusions of pure CO2 are abundant in the mineral phases in the xenoliths, and it is suggested that flux of CO2 from the mantle has been an important heat source and fluid medium during metamorphism of the mafic and ultramafic protoliths at the lower crust/upper mantle boundary.The calculated pressures and temperatures suggest that the south-eastern Australian crust has sustained a high geothermal gradient. In addition, the nature of the mineral assemblages and the contact relationships of granulitic rock with spinel lherzolite, characteristic of mantle material, suggest that the Moho is not a discrete feature in this region, but is represented by a transition zone approximately 20 km thick. These inferences are in agreement with geophysical data (including seismic, heat-flow and electrical resistivity data) determined for south-eastern Australia.Underplating at the crust/mantle boundary by continental basaltic magmas may be an important alternative or additional mechanism to the conventional andesite model for crustal accretion.

Notes: Abstract. In the Kamuikotan zone, central Hokkaido, Japan, two distinct types of metamorphic rocks are tectonically mixed up, along with a great quantity of ultramafic rocks; one type consists of high-pressure metamorphic rocks, and the other of low-pressure ones. The high-pressure metamorphic rocks are divided into two categories. (1) Prograde greenschist to glaucophaneschist facies rocks derived from mudstone, sandstone, limestone, a variety of basic rocks such as pillow and massive lavas, hyaloclastite and tuff, and radiolarian (Valanginian to Hauterivian) chert, among which the basic rocks and the chert, and occasionally the sandstone, occur as incoherent blocks (or inclusions) enveloped by mudstone. (2) Retrograde amphibolites with minor metachert and glaucophane-calcite rock, which are tectonic (or exotic) blocks enclosed within prograde mudstone or serpentinite, or separated from these prograde rocks by faults. The K-Ar ages of the prograde metamorphic rocks (72, 107 and 116 Ma on phengitic muscovites) are younger than those of the retrograde rocks (109, 132, 135 and 145 Ma on muscovites, and 120 Ma on hornblende). The low-pressure metamorphic rocks consist of the mafic members of an ophiolite sequence with a capping of radiolarian (Tithonian) chert with the metamorphic grade ranging from the zeolite facies, through the greenschist (partly, actinolite-calcic plagioclase) facies to the amphibolite (partly, hornblende-granulite) facies. The low-pressure metamorphism has a number of similarities with that described for‘ocean-floor’metamorphism. The tectonic evolution of such a mixed-up zone is discussed in relation to Mesozoic plate motion.

Notes: The rocks of the Scourian Complex have been intensively studied, but there is still no consensus as to the conditions of the granulite-facies metamorphism preserved in these rocks. Recent estimates of these conditions fall into two groups, one at 820-920°C and ca. 11 kbar and the second at ca. 1000°C and 〉12 kbar. Investigation of a variety of rocks shows that the recorded conditions vary with grain-size, with higher-grade conditions recorded by the cores of coarser (ca. 10 mm) crystals, and lower-grade conditions recorded by the rims of coarser grains and by finer grains. This observation suggests that re-equilibration during recovery of these rocks to the surface has been important which may account for the discrepancy in estimated P-T conditions. Revised estimates of the equilibration conditions of the Scourian Complex of T 〉 1000°C and P 〉 8.5 kbar are presented. The conditions suggested for the peak of metamorphism mean that the role of anatexis in the genesis of these rocks must be considered and the nature of the fluid phase thoroughly investigated.

Notes: Abstract. Plagioclase porphyroblasts from silvergrey schists belonging to the Nevado Filabride Complex in the Sierra Alhamilla (Betic Zone, SE Spain) are interpreted as having been formed preand synkinematically with respect to the second phase of deformation. Different types of inclusion patterns represent 'snap-shots’(high growth-rate/strain-rate ratio features) of the formation of a diffentiated crenulation cleavage during this second phase of deformation, by the processes of kinking, crenulation and associated differentiation.Regional considerations indicate an Alpine age for this tectono-metamorphic event, which can be explained by the‘hot emplacement’of the higher Nevado Filabride units. The observed structural evolution is not consistent with a pre-Alpine polyphase deformation history.

Notes: Evidence from rock microstructures, mass transfer and isotopic exchange indicates that substantial quantities of aqueous fluids are involved in low- and medium-grade regional metamorphism. Similar conclusions are drawn from many retrograde environments, whereas high-grade metamorphic fluids may be melt dominated. The mobile fluids play essential roles in metamorphic reactions, mass transport and deformation processes. These processes are linked by the mechanical consequences of metamorphic fluid pressures (Pf) generally being greater than or equal to the minimum principal compressive stress. Under such conditions metamorphic porosity comprises grain boundary tubules and bubbles together with continuously generated (and healed) microfractures. Deformation results in significant interconnected porosity and hence enhanced permeability. Lithologically and structurally controlled permeability variations may cause effective fluid channelling.Simple Rayleigh-Darcy modelling of a uniformly permeable, crustal slab shows that convective instability of metamorphic fluid is expected at the permeabilities suggested for the high Pf metamorphic conditions. Complex, large-scale convective cells operating in overpressured, but capped systems may provide a satisfactory explanation for the large fluid/rock ratios and extensive mass transport demonstrated for many low- and medium-grade metamorphic environments. Such large-scale fluid circulation may have important consequences for heat transfer in and the thermal evolution of metamorphic belts.

Notes: Hydrothermal synthesis of Fe-pum-pellyites was conducted using high pressure cold-seal apparatus and solid oxygen buffering techniques at temperatures between 250°C and 550°C and 2.0–9.1 kbar Pfluid. Fe-pumpellyites were synthesized from partially crystalline gel mixtures of compositions: 4CaO - 2.1Al2O3_1.5FeO - 0.3MgO - 6SiO2 (II) and 3CaO - 1.5 Al2O3 - 2.7FeO - 0.3MgO - 6SiO2 (III) in the presence of excess H2O at Pfluid of 5–9.1 Kbar, temperatures between 275°C and 325°C, and fO2 defined by the QFM and HM buffers; for both of these compositions (II and III), the condensed synthetic run products included minor 7Å chlorite ± garnet ± Fe-oxide. The cell dimensions and aggregate refractive index (a= 19.13(2)Å, b= 5.940(4)Å, c= 8.847(5)Å, ±= 97.37(6)±, and n= 1.702(2)) of the pum-pellyite synthesized from the bulk composition II mix are compatible with those of natural pumpellyites containing similar total Fe contents. Attempts at synthesizing Fe-pumpellyites from a Mg-free bulk composition were not successful; these results are consistent with the total absence of natural Mg-free pumpellyites.The higher temperature, higher oxygen fugacity assemblages of the equivalent bulk compositions (II and III) consist of epidote ± minor amounts of chlorite, garnet, quartz, hematite, and magnetite. The results of these synthesis experiments accord with the mineral parageneses observed in low-grade metabasites which imply that Fe-pumpellyites are replaced by epidote with increasing temperature and/or fO2 and that Fe3+ is preferentially partitioned into epidote with respect to coexisting pum-pellyite. In addition, these synthesis experiments indicate that Fe-bearing pumpellyites crystallize at and are stable to lower temperatures than more aluminous pumpellyites—a result also consistent with natural systems.

Notes: The author outlines his experiences of photographic surveying, in the early years of the 1930's in the Survey of India, which involved the use of simple graphical methods of plotting.

Notes: Aerial photography may be used to quickly locate and measure the extent and severity of forest damage due to windblow. This paper describes such an application in a small area of semi-natural woodland in central Scotland after the “Great Gale” of 14th and 15th January, 1968. A 1 : 2000 scale map, showing the location and direction of fall of each windblown tree, suggests certain relationships between topography and the severity of woodland damage and gives considerable information on the airflow pattern existing over the area during the gale.

Notes: The International Society for Photogrammetry held the XIIth International Congress at Ottawa during July and August 1972. The authors comprised a panel which engaged in discussion upon the Congress at a meeting of the Photogrammetric Society on 17th October, 1972.

Notes: A phototheodolite was used during the construction of Llyn Brianne dam to provide stereoscopic coverage of both upstream and downstream shoulders of the dam at each of eight stages of construction. Ground control was established on the valley sides, together with 80 targeted points at various levels on the dam as it progressed. The co-ordinates of the targeted points were determined numerically using a Wild A7. Three dimensional displacements were determined from the change of position to an average accuracy of 0–05 m. Agreement was obtained with independent and more precise measurements and comparisons are made with the upstream displacements. The assumptions of an analytical prediction technique are examined. Profile lines through the dam were also obtained numerically to record the progressing shape of the dam.

Notes: Book reviews in this article:A LAND SYSTEM ATLAS OF WESTERN KENYA. By R. M. Scott, R. Webster and C. J. Lawrance.THE OVERHAUL OF THE 1 : 2500 COUNTY SERIES OF MAPS. Ordnance Survey Professional Paper No. 25, Ordnance Survey of Great Britain, Southampton.TERRAIN ANALYSIS: A GUIDE TO SITE SELECTION USING AERIAL PHOTOGRAPHIC INTERPRETATION. By D. S. Way.PROCEEDINGS OF MAN-MACHINE INTERFACE IN PHOTOGRAM-METRY. Edited by E. Dorrer.ELEMENTARY AIR SURVEY. By W. K. Kilford.CARTOGRAPHIE GÉNÉRALE. Tome II: Méthodes et techniques de production. By R. Cuenin.INTERNATIONAL YEARBOOK OF CARTOGRAPHY (Vol. XII, 1972). Edited by E. Arnberger and F. AuradaRESOURCE SATELLITES AND REMOTE AIRBORNE SENSING FOR CANADA. Proceedings of the First Canadian Symposium on Remote Sensing, Ottawa, February 1972. Edited by D. White.LIBYA: AGRICULTURE AND ECONOMIC DEVELOPMENT. Edited by J. A. Allan, K. S. McLachlan and E. T. Penrose.ÉTUDE EXPÉRIMENTALE DE ĽAÉROTRIANGULATION SEMI-ANALYTIQUE. RAPPORT SUR ĽESSAI “GRAMASTETTEN”. Publication Officielle No. 7 de ľOrganisation Européenne ďÉtudes Photo-grammétriques Expérimentales. By P. Wiser.COORDINATE SYSTEMS AND MAP PROJECTIONS. By D. H. Maling.

Notes: Several problems are encountered when collecting traffic information by conventional methods and which leave scope for the development of more comprehensive techniques. This paper reviews the potential of different types of photography as an effective means of obtaining comprehensive traffic information. Comparisons between conventional methods and photographic methods are made. The different types of available photography are discussed, together with their uses in the field of traffic data acquisition. Finally, the paper assesses the use of automatic and semiautomatic methods of photographic data analysis in this context.

Notes: Book reviews in this article:PHOTOGRAMMETRIC GUIDE. By J. Albertz and W. Kreiling.THE READJUSTMENT OF THE RETRIANGULATION OF GREAT BRITAIN, AND ITS RELATIONSHIP TO THE EUROPEAN TERRESTRIAL AND SATELLITE NETWORKS. By V. Ashkenazi, P. A. Cross, M. J. K. Davies and D. W. Proctor.GUIDE TO SITE SURVEYING. Edited by R. Hewitt.PHOTOGRAMMÉTRIE GÉNÉRALE. By H. Bonneval.

Notes: This paper is concerned with a new technique (the terrestrial/photogrammetric (TP) technique) for the detection and compensation of systematic height errors in block aerial triangulation. The technique improves the height accuracy of such triangulation by reducing the original bridging distance, using new bands of corrected photogrammetric tie points as height control together with the original bands of the terrestrial (or ground) height control. A number of photogrammetric blocks with different characteristics and configurations have been tested in a comprehensive manner. The tests show that the TP technique gives consistent results over a wide range of circumstances and that it is a most effective, simple and inexpensive method for the compensation of systematic errors.〈section xml:id="abs1-2"〉〈title type="main"〉RésuméCet article relate une technique nouvelle permettant de détecter et de compenser les erreurs altimétriques systématiques dans l'aérotriangulation par blocs: la technique terrestre/photogrammétrique (TP). Cette technique améliore la précision altimétrique de l'aérotriangulation en réduisant la distance de l'aérocheminement original, grâce à l'utilisation d'un canevas de points d'appuis altimétriques composés de points de liaison photogrammétriques corrigés, en supplément du canevas original de points d'appui altimétriques déterminés sur le terrain.On a essayé de manière approfondie un grand nombre de blocs dotés de caractéristiques et de configurations diverses. Les essais montrent que la technique TP fournit des résultats fiables dans un large éventail de circonstances et que c'est une méthode de compensation des erreurs systématiques efficace, simple et peu coûteuse.〈section xml:id="abs1-3"〉〈title type="main"〉ZusammenfassungIm Artikel wird ein neues Verfahren behandelt (das terrestrisch-photogrammetrische Verfahren (TP)), das zur Aufdeckung und Kompensation system-atischer Höhenfehler in der Block triangulation dient. Es wird die Höhengenauigkeit durch Verkürzung der ursprünglichen Überbrückungsdistanz verbessert, indem neue Streifen korrigierter Verbindungspunkte als Höhenpasspunkte zusammen mit den ursprünglichen Reihen der terrestrischen Höhenpasspunkte verwendet werden. Eine Anzahl photogrammetrischer Blöcke unterschiedlicher Figur und Charakteristik wurde umfassend getestet. Die Tests zeigen, dass die TP-Methode konstante Ergebnisse für einen grossen Bereich van Möglichkeiten ergibt und dass es ein höchst effektives, einfaches und billiges Verfahren zur Eliminierung systematischer Fehler ist.

Notes: During June and July 1981, synthetic aperture radar imagery was acquired of selected sites in ten European countries as part of the European SAR 580 campaign. Evaluation of this imagery in the UK will be carried out by 18 approved experiments (out of 56 for Europe) when the processed data becomes available to investigators. To assist the evaluation process, a review is given of some recent research on applications of imaging radar in agricultural and urban areas. Research is described which considered the effects of terrain parameters and radar parameters on crop classification accuracy. The relative merits of a quantitative or a qualitative approach in classification of radar data are considered. Combined use of radar and Landsat data achieves a marked improvement in classification accuracy. The development of spatial filtering techniques may assist in the problem in urban areas resulting from the dependence of image tone on the relative alignment of ground features and radar look direction.〈section xml:id="abs1-2"〉〈title type="main"〉RésuméPendant les mois dejuin et juillet 1981, on a, dans le cadre de la campagne européenne SAR 580, recueilli des données de radar imageur à synthèse d'ouverture sur des sites choisis dans une dizaine de pays d'Europe. Ces données seront évaluées au Royaume-Uni au moyen de 18 expériences ayant été acceptées (sur un total de 56 menées en Europe); ce travail commencera dès que les données une fois traitées seront distribuées aux chercheurs. Pour accompagner cette procédure d'evaluation, on fournit une analyse de quelques recherches récentes sur les applications de l'imagerie radar aux zones agricoles et urbaines.L'une de ces recherches prenait en compte les effets des paramètres liés au terrain sur l'exactitude de la classification des cultures. Les mérites réciproques des approches quantitatives et qualitatives dans la classification des données radar sont également examinés.Le recours à une combinaison de données radar et Landsat permet d'améliorer de manière significative l'exactitude de la classification. La mise en oeuvre des techniques de filtrage spatial peut aider à réduire, dans les zones urbaines, les conséquences néfastes sur la tonalité de l'image, d'objets au sol en alignement relatif avec la direction d'observation du radar.〈section xml:id="abs1-3"〉〈title type="main"〉ZusammenfassungIm Juni und Juli 1981 erfolgten Radaraufnahmen mittels synthetischer Apertur von ausgewählten Gebieten in 10 europäischen Ländern als Teil der Europäischen SAR 580 Kampagne. Die Analyse dieser Aufzeichnungen wird im Vereinigten Königreich durch 18 (aus insgesamt 56 für Europa) bestätigte Experimente erfolgen, nachdem die Daten zur Verfügung stehen. Um den Analysenprozess zu unterstützen, wird ein Überblick über kürzliche Forschungen zu Radaraufzeichnungen in landwirtschaftlichen und städtischen Gebieten gegeben. Es werden auch Forschungen beschrieben, bei denen die Einflüsse von Geländeparametern auf die Genauigkeit der Ernteklassifizierung untersucht wurden. Der relative Wert eines quantitativen oder qualitativen Versuchs zur Klassifizierung von Radardaten wird abgeschätzt. Eine Kombination von Radarund Landsat-Daten ergibt eine merkliche Verbesserung bei Klassifizierungsgenauigkeit. Die Entwicklung von Verfahren der räumlichen Filterung kann von Wert bei städtischen Gebieten sein, weil es eine Abhängigkeit des Grauwertes von der relativen Ausdehnung von Geländecharakteristika und der Richtung der Radaraufnahme gibt.

Notes: Some aspects of the XYZBLC suite of programs for aerial triangulation are described. The Ordnance Survey Bute block of photography at a scale of 1: 24000 is computed by independent models using XYZBLC. Three of the strips in the block are treated as a test block and are computed with different configurations of control. Conclusions are drawn with regard to the usefulness of height control doublets and other dense control configurations.〈section xml:id="abs1-2"〉〈title type="main"〉Résumé Quelques aspects de l'ensemble XYZBLC de logiciels d'aérotriangulation sont décrits. Le bloc Bute de l'Ordnance Survey, composé de photographies à l'échelle du 1: 24000, a été calculé par modèles indépendants avec les logiciels XYZBLC. On a considéré trois des bandes du bloc comme formant un bloc d'essai et on les a calculées avec diverses combinaisons de canevas de points d'appui. On en tire des conclusions quant à l'utilité des doublets de points d'appui altimétriques et de diverses autres configurations denses de canevas d'appui. 〈section xml:id="abs1-3"〉〈title type="main"〉Zusammenfassung Beschreibung einiger Aspekte des XYZBLC-Programmsystems zur Aero-triangulation. Ein Block des Ordnance Survey im Bildmassstab 1: 24000 wurde damit nach der Methode der unabhängigen Modelle berechnet. Drei der Streifen des Blocks wurden als Testblock behandelt und mit verschiedenen Passpunktanordnungen berechnet. Es werden Schlussfolgerungen in bezug auf die Nützlichkeit von Zwillingspunkten zur Höhenorientierung und andere dichte Passpunktanordnungen gezogen.

Notes: The wide availability of reconnaissance frame photography, together with the advantage of high image resolution that such photography provides and the extreme flight conditions under which it can be taken, means that it is of great interest to photogrammetrists. However, the geometry of the image is invariably distorted due to the mode of operation of the camera. In this paper, the effects of the focal plane shutter and apparent image motion compensation on the geometry of the photographs are considered.〈section xml:id="abs1-2"〉〈title type="main"〉RésuméLe fait que la photographic de reconnaissance soil très répandue, conjointe-ment aux avantages que présente la grande résolution des images fournies par ces systèmes et les conditions extrêmes de vol dans lesquelles on peut les prendre, milite pour une prise en compte de lew intérêt par les photogrammètres. Toutefois la géométrie de ces images est systématiquement déformée par suite du mode de fonctionnement de la chambre. On traite dans cet article des effets de l'obturateur focal et de la compensation du filé de l'image sur la géométrie des photographies.〈section xml:id="abs1-3"〉〈title type="main"〉ZusammenfassungDie grosse Verbreitung von Erkundungsfotos zusammen mit der grossen Auflösung solcher Fotos und den extremen Bedingungen unter denen sie auf-genommen wurden, lassen diese auch für den Photogrammeter interessant erscheinen. Die Geometrie dieser Bilder ist jedoch infolge der speziellen Kamera-funktion verzerrt. Im Artikel werden die Einflüsse des Schlitzverschlusses und des Bildwanderungsausgleichs auf die Bildgeometrie untersucht.

Notes: Book Reviewed in this article:AERIAL PHOTOGRAPHY. By G. S. Kumar.MANUAL OF PHOTOGRAMMETRY (Fourth Edition). Editor-in-Chief: C. C. Slama.

Notes: The authors describe the photogrammetric procedures involved in the production of 1 : 50 000 scale mapping within the Directorate of Overseas Surveys, making specific reference to a project in Ethiopia.

Notes: The paper reviews the status of the optical transfer function (OTF) as a potential standard method for measuring the definition of photogrammetric camera lenses. The nature of the OTF and its relation to resolving power are explained. The limited success in obtaining agreement in international comparisons of OTF measurement and the programme of the Scientific Instrument Research Association group research project on the O TFare summarised. Some of the difficulties envisaged in applying the OTF as an ISP standard are discussed.

Notes: Orthophotomaps at 1 : 2500 scale, contoured at 1 m vertical intervals, have been produced for an area of 52 km2 from 1 : 10 000 scale photography especially flown for the project. Planimetric and height information on published Ordnance Survey 1 : 2500 scale maps was used as control for the aerial triangulation on which the orthophotomapping was based.

Notes: The paper describes a method for the automatic determination of height profiles in the stereomodel. The model is scanned automatically in epipolar planes. The photographic densities are recorded. The density values are correlated, either on or off line, to obtain the height profiles. The method was developed for the analytical plotter but can be applied to other photogrammetric instruments.

Notes: Experiments are described which compare point transfer from one photograph to another by stereoscopic and non-stereoscopic procedures. It is shown that, whereas it may be possible to achieve very high accuracies by both methods, the former method is more dependent for its accuracy on a high observer skill and is also more prone to occasional mistakes in identification.

Notes: Methods and results of practical applications of block adjustment by independent model cadastral surveys are described. The wider implications of the results are assessed in conjunction with current research trends in the adjustment of extremely large blocks for small scale mapping and in the use of the bundle adjustment method.

Notes: Digitising from a photogrammetric instrument can be controlled by a unit specially designed for this purpose, or by a digital control computer. In the latter case, the digitising system is more flexible since alterations and additions to the real-time programs can easily be made to effect different digitising applications. The control computer can also be used in processing the digitised data.The use of such digital control in photogrammetry is as yet limited. The reasons are examined. The characteristics and real-time requirements of a computer controlled digitising system are stated. Different digitising and recording modes, identification numbers and editing arrangements are then given to illustrate the flexibility and limitations of such a system.

Notes: Underwater photogrammetry is being used in the particularly hostile environment of the North Sea where it has been put into commercially viable operation. The authors discuss underwater photogrammetry related to other means of inspection of oil platforms. They also deal with operational experience of 35 mm and 70 mm cameras, camera calibration, the provision of control and photogrammetric observations and data analysis. The demand for underwater photogrammetry continues to grow.

Notes: This paper summarises the theoretical and practical development of’an analytical system for close range photogrammetry. The system uses the extended bundle method of phototriangulation and a sophisticated least squares algorithm. A description is given of computer software, and aspects of statistical testing and gross error detection are discussed in order to assess the accuracy potential. Applications of the system, for precise co-ordination of a target range and camera calibration are outlined.

Notes: A photogrammetric system that is suitable for shallow water detailed mapping at scales from 1:50 to 1:500 for coastal and harbour engineering projects is described. The results from tests using automatic 35 mm Canon cameras on an elevated platform to determine the accuracy of planimetric and depth co-ordinates are presented. The economic benefits of stereophotography through shallow water rather than underwater photography are discussed.

Notes: This paper reviews the growth in the use of photogrammetric techniques in architecture, principally in the United Kingdom, over the past ten years. The work of the various establishments involved in architectural photogrammetry is described and the results of a questionnaire, distributed within the architectural profession, are analysed. Some comparisons are made with developments elsewhere in Europe.〈section xml:id="abs1-2"〉〈title type="main"〉RésuméOn examine dans cet article l'extension qu'a connue l'utilisation des méthodes photogrammétriques en architecture au cows des dix dernières années, principalement dans le Royaume-Uni. On y décrit les travaux des divers organismes impliqués dans la photogrammétrie architecturale et l'on y analyse les résultats d'un questionnaire diffusé au sein de la profession liée à l'architecture. On fournit enfin quelques comparaisons avec les développements constatés par ailleurs en Europe.〈section xml:id="abs1-3"〉〈title type="main"〉ZusammenfassungIm Artikel wird über die zunehmende Anwendung photogrammetrischer Verfahren während der letzten 10 Jahre in der Architektur, insbesondere im Vereinigten Königreich, berichtet. Die Arbeit verschiedener Institutionen, die sich mit Architekturphotogrammetrie befassen, wird beschrieben, und es werden die Ergebnisse eines unter Architekten verteilten Fragebogens analysiert. Weiterhin werden Vergleiche zu Entwicklungen in anderen europäischen Ländern gezogen.

Notes: The algorithm presented assumes a preliminary determination of the elements of inner orientation and bases its mathematical model on the collinearity condition, from which projective equations are derived. Linearisation of condition equations is based on Taylor's expansion, whilst the subsequent solution follows iteration cycles. The method aims to derive composite corrections to image coordinates, with the aid of test field data.〈section xml:id="abs1-2"〉〈title type="main"〉RésuméL'auteur présente un algorithme oú l'on suppose déterminés au préalable les éléments d'orientation interne. La modélisation mathématique s'appuie sur les conditions de co-linéarité et sur les équations qui en résultent en projection. On linéarise les équations de conditions en utilisant un développement en série de Taylor, et l'on obtient la solution correspondante par itération. La méthode vise à apporter des corrections aux coordonnées images en utilisant les données d'un polygone d'essai.〈section xml:id="abs1-3"〉〈title type="main"〉ZusammenfassungBei dem dargestellten Algorithmus wird die vorläufige Bestimmung der Elemente der inneren Orientierung angenommen, und das mathematische Modell basiert aufder Kollinearitätsgleichung, woraus perspektive Gleichungen abgeleitet werden. Die Linearisierung der Bedingungsgleichungen erfolgt durch Anwendung der Taylor-Reihe, was für die folgenden Berechnungen einen iterativen Prozess bedingt. Mit der Methode wird angestrebt, Bildkoordinaten mit Hilfe von Testfelddaten zu verbessern.

Notes: There is a growing interest in the potential of synthetic aperture radar (SAR) for mapping and for gathering intelligence for map revision. This paper first gives a simple guide to the characteristics of SAR and then describes a study carried out to investigate the geometric properties of the imagery and the information content which is of interest for topographic mapping and the production of large scale plans. The conclusions cautiously indicate a potential although better quality images are required and more information must be known about the optimum conditions which should obtain when the data are recorded.〈section xml:id="abs1-2"〉〈title type="main"〉RésuméOn assiste à un intérét croissant envers le radar à ouverture synthétique (SAR) et ses possibilityés pour la cartographie et pour la collecte de renseignements utiles à la révision. Ce texte commence par donner un simple rappel des caractéristiques de ces radars pour décrire ensuite une étude effectuée sur le contenu informatif et les proprietyés géométriques de l'imagerie radar, qui intèresse la cartographie topographique et la confection de plans à grande échelle.Les conclusions font prudemment état d'une potentialité mais soulignent qu'il faudrait disposer d'images de meilleure qualityé et avoir davantage d'informations sur les conditions optimales à respecter lors de l'enregistrement des données.〈section xml:id="abs1-3"〉〈title type="main"〉ZusammenfassungEs besteht zunehmendes Interesse an den Möglichkeiten von Radaraufnahmen mittels synthetischer Apertur (SAR) zur Kartenherstellung und Erfassung von Daten zur Laufendhaltung. Zunächst wird im Artikel eine einfache Einführung in die SAR-Charakteristika gegeben. Dann erfolgt eine Beschreibung eines Versuches zur Untersuchung der geometrischen Eigenschaften und des Informationsgehalts, der von Interesse für die Herstellung topographischer und grossmassstäbiger Karten ist. Vorsichtige Schlussfolgerungen ergeben Möglichkeiten zur Verwendung solcher Informationen, wobeijedoch eine bessere Bildqualität zu fordern ist. Auch müssen noch mehr Kenntnisse über die optimalen Bedingungen bezüglich der Datenregistrierung bestehen.

Notes: Landsat multispectral scanner (MSS) and return beam vidicon (RBV) image data in both analogue and digital formats were used to assess the performance of the MSS and RBV sensor systems, and to determine their potential for recording information associated with topographic or land use maps of selected Chinese study areas (Beijing, Tianjin and the Huang He elbow). MSS images permitted the detection of 40 per cent to 50 per cent of the features identified on maps of 1: 250000 scale and larger, whereas the higher resolution Landsat 3 RBV images provided 10 per cent to 20 per cent additional information. Completeness of information extracted from a colour cathode ray tube display exceeded that obtained from analogue single band black and white MSS images by only 5 per cent to 10 per cent. Only through major improvements in spatial resolution will it be possible to compile maps at scales of 1: 250000 and larger from satellite data. Significantly, it appears that instantaneous field of view values of 5 m to 10 m will be required to produce image data of sufficient resolution for topographic mapping or detailed thematic studies of the Asian environment.〈section xml:id="abs1-2"〉〈title type="main"〉Résumé On a utilisé les données de l'imagerie spatiale provenant du scanneur multispectral (MSS) et du tube vidicon à retour de faisceau (RBV) de Landsat, à la fois sous forme analogique et numérique, pour évaluer la performance des systèmes de capteurs MSS et RBV, et pour déterminer leur aptitude à contenir l'information figurant dans les cartes topographiques et les cartes d'occupation du sol, sur des polygones d'essais sélectionnés en Chine (Pekin, Tianjin, et le coude du Huan He). Les images MSS ont permis de détecter 40 à 50 pour cent des détails figurant sur les cartes à une échelle supérieure ou égale à 1: 250000, tandis que les images RBV à meilleure résolution de Landsat C fournissaient 10 à 20 pour cent d'informations supplémentaires. Le volume des informations extraites sur un écran cathodique de visualisation en couleurs n'a dépassé le volume obtenu à partir d'images d'une seule bande MSS en noir et blanc que de 5 à 10 pour cent seulement. Il semble que ce ne soit que par des améliorations importantes dans la résolution spatiale que l'on pourra tirer des données de satellites des cartes à des échelles supérieures ou égales à 1: 250000. Il apparait de manière significative qu'un champ instantané de prise de vues de 5 à 10 m (pixel) sera nécessaire pour obtenir des images ayant une résolution suffisante pour la cartographie topographique ou pour des études thématiques détaillées sur l'environnement asiatique.〈section xml:id="abs1-3"〉〈title type="main"〉Zusammenfassung Verwendung von Landsat-aufzeichnungen, die mit Hilfe des multispektralen Scanners (MSS) und des Return Beam Vidicon (RBV) aufgenommen wurden und sowohl in analoger als auch digitaler Form vorlagen. um die Leistungsfähigkeit dieser Aufnahmesysteme zu ermitteln und die Möglichkeiten zur Gewinnung von Informationen zur Herstellung von topographischen oder Landnutzungskarten in ausgewählten chinesischen Versuchsgebieten (Beijing, Tianjin undder Huang He Bogen). MSS-Bilder gestatteten die Erkennung von 40 bis 50% der auf Karten im Massstab 1: 250 000 oder grösser identifizierten Objekte, während die RBV-Bilder von Landsat 3, die eine grössere Auflösung hatten, 10 bis 20% zusätzliche Informationen lieferten.Die Vollständigkeit der Information, die von einem Farbdisplay erhalten wurde, überstieg die von analogen einkanaligen schwarzweissen MSS-Bildern erhaltenen nur um 5 bis 10%. Nur durch wesentliche Verbesserungen der Objektauflösung wird es möglich sein, Karten 1: 250000 und grösser nach Satellitendaten herzustellen. Es wird offensichtlich eine Objecktauflösung von 5 bis 10m gefordert, um Bilddaten genügender Auflösung für die Herstellung topographischer Karten oder thematischer Studien des asiatischen Raums zu erzeugen.

Notes: One third of a collection of cloned Stylonychia pustulata micronuclear DNA PstI fragments were found to be of a similar size, consistent with their being members of a repetitious sequence family with a repeat size of about 160 base pairs. Cross-hybridization experiments confirmed that these small cloned fragments are related by sequence homology. Hybridization of the cloned repetitious sequences to PstI digested micronuclear DNA revealed a “ladder” of bands (step size = 160 base pairs), indicating that the repeats are found in tandem arrays. This is the first demonstration of highly repetitious, tandemly repeated sequences in a ciliated protozoan.

Notes: The erythrocytic developmental cycle of Plasmodium falciparum can be conveniently divided into the ring, trophozoite, and schizont stages based on morphology and metabolism. Using highly synchronous cultures of P. falciparum, considerable variation was demonstrated among these stages in sensitivity to chloroquine. The effects of timed, sequential exposure to several clinically relevant concentrations of chloroquine were monitored by three techniques: morphological analysis, changes in the rate of glucose consumption, and changes in the incorporation of 3H-hypoxanthine into parasite nucleic acids. All three techniques gave essentially identical results. The trophozoite and schizont stages were considerably more sensitive to the drug than ring-stage parasites. Chloroquine sensitivity decreased as nuclear division neared completion. The increase in chloroquine sensitivity was coincident with a marked rise in the rate of glucose consumption and nucleic acid synthesis. The rate of nucleic acid synthesis decreased as schizogony progressed while glucose consumption continued at high rates during this process. The degree of chloroquine sensitivity was not highly correlated with either metabolic activity.

Notes: Bloodstream trypomastigote and culture procyclic (insect midgut) forms of a cloned T. rhodesiense variant (WRATat 1) were tested for agglutination with the lectins concanavalin A (Con A), phytohemagglutinin P (PP), soybean agglutinin (SBA), fucose binding protein (FBP), wheat germ agglutinin (WGA), and castor bean lectin (RCA). Fluorescence-microscopic localization of lectin binding to both formalin-fixed trypomastigotes and red cells was determined with fluorescein isothiocyanate (FITC)-conjugated Con A, SBA, FBP, WGA, RCA, PNA (peanut agglutinin), DBA (Dolichos bifloris), and UEA (Ulex europaeus) lectins. Electron microscopic localization of lectin binding sites on bloodstream trypomastigotes was accomplished by the Con A-horseradish peroxidase-diaminobenzidine (HRP-DAB) technique, and by a Con A-biotin/avidin-ferritin method. Trypomastigotes, isolated by centrifugation or filtration through DEAE-cellulose or thawed after cryopreservation, were agglutinated by the lectins Con A and PP with agglutination strength scored as Con A 〈 PP. No agglutination was observed in control preparations or with the lectins WGA, FBA or SBA. Red cells were agglutinated by all the lectins tested. Formalin-fixed bloodstream trypomastigotes bound FITC-Con A and FITC-RCA but not FITC-WGA, -SBA, -PNA, -UEA or -DBA lectins. All FITC-labeled lectins bound to red cells. Con A receptors, visualized by Con A-HRP-DAB and Con A-biotin/avidin-ferritin techniques, were distributed uniformly on T. rhodesiense bloodstream forms. No lectin receptors were visualized on control preparations. Culture procyclics lacked a cell surface coat and were agglutinated by Con A and WGA but not RCA, SBA, PP and FBP. Procyclics were not agglutinated by lectins in the presence of competing sugar at 0.25 M. The expression of lectin binding cell surface saccharides of T. rhodesiense WRATat 1 is related to the parasite stage. Sugars resembling α-D-mannose are on the surface of bloodstream trypomastigotes and culture procyclics; n-acetyl-D-galactosamine and D-galactose residues are on bloodstream forms; and n-acetyl-D-glucosamine-like sugars are on procyclic stages.

Notes: The cationic permeant fluorescent dye rhodamine 123 (R123) was used to stain Plasmodium yoelii-infected mouse erythrocytes. Fluorescence microscopic observations demonstrated that the parasite, but not the matrix of the infected erythrocyte, accumulated the dye. Differences in fluorescence intensity could not be found at the various developmental stages of the parasite; however, quantitation of the cell-associated dye revealed an increase in R123 uptake with parasite development. The retention of the parasite-associated dye, as measured by fluorescence microscopy and spectrophotometry after extraction of R123 with butanol, was markedly reduced by treatment of the infected erythrocytes with a proton ionophore, carbonyl cyanide m-chlorophenylhydrazone (CCCP), and an inhibitor of proton ATPase, dicyclohexylcarbodiimide (DCCD). These results indicate that the accumulation and retention of R123 in P. yoelii reflect the parasite membrane potential and suggest that the parasite plasma membrane has a membrane potential-generating proton pump.

Notes: Distinctive organic-walled resting cysts of at least three different types with a highly conservative morphology appear to characterize specific orders or groups of genera within the Class Polyhymenophorea (Protozoa, Ciliophora), contrasting markedly with the great diversity of form seen in trophic stages. Polyhymenophorean ciliates have been considered in the past to form a cohesive class within the Phylum Ciliophora and, possibly, to represent the pinnacle of ciliate evolution. Evidence from cysts challenges the cohesive nature of the class, suggesting that the hypotrichs should be subdivided and that they have a different phylogenetic origin from the heterotrichs, tintinnids, and oligotrichs.

Notes: Yellow-brown, algal symbionts varying in diameter from approximately 5 μ m to 20 μ m, associated with solitary Radiolaria with spongiose skeletons (i.e. Spongodrymus sp.), exhibit fine structural features resembling the Prymnesiida (botanical class, Prymnesiophyceae). A large central vacuole is surrounded by a thin layer of cytoplasm containing plastids with lamellae composed of three thylakoids and granular pyrenoids with internal tubules immersed between the thylakoids. The pyrenoids lack internal thylakoid membranes. The nucleus is surrounded by a dilated cisterna of the nuclear envelope that also encloses the plastids and gives rise to saccules of the endoplasmic reticulum. The algal symbionts appear coccoid; hence no flagella nor surface scales were observed. The symbiont fine structure is compared to similar yellow-brown symbionts associated with Acantharia. Thus far, three kinds of algal symbionts have been observed to be associated with solitary Radiolaria: dinoflagellate, prasinomonad, and this apparent prymnesiomonad.

Notes: Ultrastructural observations of the cortically-located mitochondria of Tetrahymena thermophila revealed associations not only between the mitochondria and certain of the cortical microtubule bands, but also between the mitochondria and the epiplasm of the cortex. Most of the distal mitochondrial surface is close and parallel to the epiplasm; favorable views show bridge-like structures spanning the 20–10 nm gap between the mitochondrion and the epiplasm.Previous studies have shown that the placement of mitochondria in the cortex appears to be determined by certain of the cortical microtubule bands. This study, however, shows that mitochondrion-microtubule interactions account for only a small proportion of the total mitochondrial area associated with the cortex; the rest is accounted for by the epiplasm. A possible analogue of the spectrin layer of erythrocyte membranes, the epiplasm may be important in helping to arrange the intricately organized components of the ciliate cortex. Its involvement in apparently helping to “moor” mitochondria to their cortical sites is the first suggestion of any role in cell patterning played by the epiplasm.

Notes: Ce Tetradimorpha, rencontre en eau douce se présente soit sous forme sphérique pourvue de quatre flagelles et d'axopodes rayonnants, soit sous forme allongée avec a l'avant quatre flagelles associes a quatre axopodes et a l'arriére six a huit axopodes divergents. L'etude ultrastructurale révèle un cytosquelette axopodial de type centroplastidie comprenant un centroplaste lenticulaire homogéne, centre organisateur des quatre axopodes anterieurs et des six a huit axopodes posterieurs, auquel s'ajoutent les quatre cinetosomes des flagelles anterieurs. En outre, un deuxiéme éleément cytosquelettique incluant un microtubule associe chacun des quatre cinetosomes a l'axopode antérieur correspondant. Des cordons microfibrillaires réunissent axopodes et cinetosomes au niveau du centroplaste, puis a quelque distance du centroplaste les axopodes posterieurs. Les axonémes des axopodes comprenant de 5 a 30 microtubules sont constitues de triades, lorsqu'on peut détecter une organisation. Le noyau, a nucléole central est coince dans le cone axopodial posterieur, lui-méme entouré des dictyosomes. Par l'organisation du cytosquelette, par la structure des kinétocystes, par la structure des flagelles dépourvus de mastigonémes tubulaires, Tetradimorpha différe nettement de Ciliophrys marina. Comme le prévoyait Davidson (1975), il represönte bien un des chainons dans la série évolutive des Héliozoaires centrohélidiens. Mais il ne présente guère d'affinites avec les Chrysomonadines considerees comme la souche des Héliozoaires. L'intéret de ce Protiste dans l'étude de la differentiation et de l'evolution du cytosquelette est également présente.〈section xml:id="abs1-2"〉〈title type="main"〉ABSTRACTThis freshwater species of Tetradimorpha has a spherical body with four flagella and radiating axopods; it transforms into a pear-shaped cell that anteriorly has four flagella intercalated between four axopods and posteriorly has six to eight divergent axopods. Ultrastructural study reveals an axopodial cytoskeleton of the centrohelidan type comprising an homogeneous lenticular centroplast which acts as MTOC for axopodial microtubules. A second skeletal element is a microtubular linkage between the kinetosomes and the axonemes of anterior axopods. A microtubule embedded in dense material diverges from near the base of each kinetosomes and parallels the distal portion of the axoneme of each anterior axopod. A microfibrillar envelope around the centroplast links the axopodial bases to the kinetosomes situated just above. Close to the centroplast, microfibrillar strands link the axopodial axonemes to the kinetosomes. Axopodial axonemes are composed of 5 to 30 microtubules irregularly arranged except for some that form equilateral triangles. The nucleus containing a central nucleolus is constrained within a cone formed by the axonemes of the posterior axopods and surrounded by dictyosomes. By the cytoskeletal organization, the structure of kinetocysts, and flagella wthout tubular mastigonemes, Tetradimorpha differs obviously from Ciliophrys marina. As Davidson (1975) predicted, Tetradimorpha is an intermediate link in the centrohelidan lineage: however, it lacks the characteristics of chrysomonads, the supposed ancestors of Heliozoa. The contribution of this genus to the study of the differentiation and the evolution of the cytoskeleton is also presented and discussed.

Notes: Two-dimensional gel electrophoresis was used to identify the patterns of protein synthesis during initiation, and the patterns of membrane protein expression following initiation, in all of the mating types of the Tetrahymena thermophila B family. In addition, one-dimensional analysis was used to survey 125I-Concanavalin A-binding proteins. Although a large number of proteins was identified by each technique, no variation among the mating types was observed.

Notes: Cells of Tetrahymena pyriformis, T. thermophila, and Euglena gracilis were saturated with nitrogen gas at pressures up to 300 atm and rapidly decompressed. Damage was assessed by measuring post-decompression cell fragmentation or viability. Occurrence of intracellular bubbles was determined by cinephotomicrography performed during the decompression or by direct observations afterwards. The extreme gas supersaturations induced led to intracellular bubble formation and rupture in cells of Tetrahymena that contained food vacuoles, but only with supersaturations of 175 atm or higher; 225 atm left few cells intact. Bubbles were never observed in cells of Euglena or in Tetrahymena cells freed of food vacuoles, even when they were decompressed from substantially higher nitrogen supersaturations. Cells of Euglena were most resistant and were unaffected by supersaturations up to 250 atm.

Notes: The rapid, synchronous differentiation of N. gruberi from amoebae to flagellates is a useful paradigm to study aspects of cell differentiation, including regulation of the synthesis of proteins that are related to the changes in cell shape and motility, which occur during differentiation. The differentiation requires synthesis of new RNA and protein molecules to accomplish defined morphogenetic events. Specific new proteins, including the tubulins that form the flagellar microtubules, are synthesized at various times during differentiation, and particular mRNA species appear and disappear. The time course of the synthesis of the α and β subunits of flagellar tubulin is paralleled by the programmed appearance and disappearance of flagellar tubulin mRNAs. The evidence supports the hypothesis that the synthesis of flagellar tubulin is regulated by the transcription, and subsequent disappearance, of flagellar tubulin mRNA. Translatable mRNAs for two calmodulin-like calcium-binding proteins appear and disappear contemporaneously with those for flagellar tubulin. During differentiation the synthesis of actin, the major protein of amoebae, is selectively shut down, and translatable actin mRNA rapidly disappears. This description of the orderly appearance, utilization, and disappearance of the mRNAs for actin, calcium-binding proteins, and flagellar tubulin during differentiation provides means and motivation to investigate the mechanisms that regulate these events.

Notes: Earlier experimental work involving macronuclear implants in Stentor coeruleus has shown that the cytoplasmic cortex of the nuclear site 1) attracts the macronucleus and 2) holds it in place during interphase. Now experiments indicate macronuclei transferred with overlying cortex elongate in the direction of the transferred cortical pigment stripes, whether or not the transferred stripes realign in the direction of the host stentor's stripes. Therefore the third function of the cortex is to determine the direction of elongation and thus assure that both daughter cells at division receive part of the macronucleus.

Notes: During an electron microscopic study of Glugea stephani, three morphologically distinct tubular appendages that are continuous with the sporoblast plasmalemma were observed. The tubules were designated as: type I, 45–50 nm in diameter and 600–900 nm in length; type II, 25–35 nm in diameter, averaging 1300 nm in length; type III, 50–70 nm in diameter and with an indeterminate length, which often exceeds 3000 nm. Type III tubules contain regularly spaced, electron-dense particles that are approximately 30 nm in diameter. Since many genera of microsporida have some type of appendage, which may eventually be utilized for taxonomic purposes, we propose the formation of a system of serially numbered detailed descriptions of these structures to promote uniformity and clarity in future publications.

Notes: Cells of Paramecium tetraurelia, stock hrd, cultured in a micro-capillary containing 1 μl fresh culture medium, expressed mating activity through the whole cell cycle. Mating-reactive G2 phase cells can conjugate with cells of other phases. The G2 phase cells, which have double (4C) the normal micronuclear DNA content, undergo pre-meiotic DNA synthesis when conjugated with G1 phase cells. The micronucleus of the progeny from the cross between a G1 and a G2 cell becomes triploid.

Notes: Buffered solutions of KCl and NaCl were tested for their stimulatory effect on the germination of variously-aged spores of Vavraia culicis. Germination was optimal in 0.2 M KCl, pH 6.5 for one isolate, and, for another isolate, peaks of germination occurred at pH 7.0 and 9.5. Spores incubated for several hours in suboptimal solutions became unable to germinate under optimal conditions. After being returned to water, they regained their ability to germinate. Calcium chloride, magnesium chloride, and ammonium chloride inhibited germination. After ingestion by mosquito larvae, spores germinated near the posterior end of the midgut.

Notes: . Leishmania tropica promastigotes transport α-aminoisobutyric acid (AIB), the nonmetabolizable analog of neutral amino acids, against a substantial concentration gradient. AIB is not incorporated into cellular material but accumulates within the cells in an unaltered form. Intracellular AIB exchanges with external AIB. Various energy inhibitors (amytal, HOQNO, KCN, DNP, CCCP, and arsenate) and sulfhydryl reagents (NEM, pCMB, and iodoacetate) severely inhibit uptake. The uptake system is saturable with reference to AIB-and the Lineweaver-Burk plots show biphasic kinetics suggesting the involvement of two transport systems. AIB shares a common transport system with alanine, cysteine, glycine, methionine, serine, and proline. Uptake is regulated by feedback inhibition and transinhibition.

Notes: . Oxytricha strains used in biochemical studies have traditionally been grown in unaerated, unagitated culture tubes or Fernbach flasks. These cultures are limited in volume to about one liter and have a very nonuniform distribution of cells, with the majority of the cells at the very top or bottom of the medium. We have found conditions in which Oxytricha can be grown in 50-liter fermentation vats. The cultures grow to a uniform density of about 6000 cells/ml.

Notes: . The fine structure of the tomite of Foettingeria actiniarum (Claparède) was examined and compared with that of other apostome tomites. This stage in the life cycle has a unique configuration of kineties that form a spiral through the cytoplasm in the interior of the body. The structure and behavior of this internal spiral were evaluated as a mechanism for the storage of kinetosomes, an adaptation to the ciliate's two-host life cycle. The spiral is composed of nine ribbons of laterally compressed kinetosomes that are in contact with a thin electron-dense fibril. Paralleling the kineties of the spiral are conspicuous, swollen lamellae of the rough endoplasmic reticulum; these lamellae contain moderately electron-dense material. The spiral is associated with the large contractile vacuole and winds about the macronucleus. The tomite of Foettingeria possesses a single, robust, caudal cilium located in a pit, along with the nozzle-like pore of the contractile vacuole. The walls of the pit contain several trichocysts arranged radially about the caudal cilium and aimed into the pit.

Notes: . Fine structural studies of a specialized vesicle system associated with the endoplasmic reticulum (ER) of exo-erythrocytic Plasmodium berghei suggest that this system may be the equivalent of a Golgi apparatus. Patches of ER, randomly distributed in the cytoplasm of developing parasites, are formed of smooth and ribosome-studded cisternae intermingled with each other. The vesicle systems are located between as well as at the edges of ER aggregates and appear to be in different stages of budding from the cisternae. Prolonged osmication reveals distinct staining of the nuclear envelope and ER of the parasites as well as part of the Golgi apparatus of the hepatocytes. However, the small vesicles associated with the parasite's ER are unstained, as are the coated vesicles in the Golgi region of the liver cell. These sites in the parasite cytoplasm seem comparable to the concave surface of the Golgi apparatus in liver cells. The pinched-off vesicles fuse with others to form the prominent peripheral vacuolization characteristic of the nearly mature exo-erythrocytic form. The formation of these peripheral vacuoles and their subsequent fusion with the parasite membrane may be an exocytosis mechanism supplying the rapidly expanding parasite with new plasma membrane material.

Notes: . Ultrastructural cytochemical techniques were used to analyze the nucleus and the kinetoplast of epimastigotes of Trypanosoma cruzi. With the use of ethanolic phosphotungstic acid, which detects basic proteins, reaction product was seen in the chromatin and at the periphery of the kinetoplast. Thallium alcoholate, which interacts with DNA, stained strongly the whole kinetoplast and the chromatin. With the use of a silver impregnation method that detects acidic nucleolar proteins, silver granules were seen preferentially located in the central region of the nucleolus. With the EDTA method, which reveals the presence of ribonucleoproteins, staining was observed in the nuclear pores. Also 6–8 nm fibrils, 25 nm and 40 nm granules, which correspond to the perichromatin fibers, interchromatin granules and the perichromatin granules, respectively, were identified in the nucleus. The EDTA method also revealed the presence of 40 nm granules in the kinetoplast. These granules were seen mainly at the two extremities of the kinetoplast. Freeze-fracture images indicate that the nuclear membrane contains ca. 9 pores/μm2 of nuclear surface area. The mean diameter of the pores was 80 nm. All these results suggest that epimastigotes of T. cruzi have a very active nucleus and a high rate of nucleocytoplasmic interchange.

Notes: . When a streptomycin-bleached mutant of Euglena gracilis strain Z was cultured in the dark at 33, 26, or 15°C, the content of paramylon was higher at lower growing temperature while that of wax esters was higher at higher temperature. Transfer of the cells grown at 33°C–15°C decreased the wax ester content while increasing the paramylon content; transfer in the reverse direction caused reverse changes. On incubation with labeled acetate, the cells grown at 33°C showed more distribution of radioactivity in wax esters than the cells grown at lower temperatures. Apparently the two energy-reserve substances have different physiological functions.

Notes: The sessiline peritrich Ellobiophrya conviva n. sp. is described from marine ectoprocts of the genus Bugula, the first report of an ellobiophryid on bryozoan hosts. The new species is distinguished from others of its genus by its different body proportions, size, host, and structure of the clasping holdfast (for which the new name cinctum is chosen). Ellobiophrya conviva has been found only on B. neritina and B. turrita and shows a marked seasonal cycle of abundance. The family Ellobiophryidae Chatton & Lwoff is revised on the basis of new information provided by E. conviva, with the single species of the genus Clausophrya removed to Ellobiophrya as E. oblida Naidenova & Zaika n. comb. The genus Caliperia Laird remains unchanged. The two genera of the revised family are distinguished from one another by differences in the structure of the cinctum. Hypotheses are advanced to explain the morphogenesis of the cinctum and the evolution of ellobiophryids from other peritrichs.

Notes: The spatial and seasonal distribution of Paramecium bursaria in two small Indiana ponds was studied using a sampling grid. Very small (5.0 ml) samples were taken so that the individual microhabitats could be studied. The results were evaluated in comparison to the data collected for the P. aurelia complex collected in the same manner and at the same sites. It was found that P. bursaria exist in a clumped distribution, but that the distribution was not very different from random. Paramecium bursaria also exist at the surface and at the mud-water interface. Temperature does not seem to play a statistically significant role in determining population size. The breeding system of P. bursaria is optimized for an outbreeding population of low density. In comparison, the species of the P. aurelia complex exist in a very clumped distribution, are found only at the mud-water interface, and are inbreeders. The evolutionary strategies of the two types of paramecia are discussed.

Notes: L'étude du caractère planctonique de différentes spores d'Actinomyxidies montre une complexité croissante dans leur adaptation au milieu aquatique. Au contact de l'eau, les trois cellules épéisporales de chaque spore se transforment en flotteurs de forme différente suivant les espèces. Ces flotteurs peuvent s'unir entre eux en un style équivelent à un quatrième flotteur ou associer diversement les huit spores issues d'un měme pansporocyste. C'est le cas dans le genre Synactinomyxon dont la diagnose est modifiée pour inclure une deuxième espèce S. Iongicauda n. sp. Un type nouveau est décrit chez lequel la preéence d'ancres à l'extrémité des cellules épisporales permet de maintenir efficacement réunies plusieurs dizaines de spores émises simultanément. Nous avons observé dans les genres Aurantiactinomyxon, Synactinomyxon, Echinactinomyxon l'emission du sporoplasme. II est libére en entier et capable de se déplacer dans l'eau pendant plus d'une heure grǎce à des mouvements amoeboïdes. Chez Aurantiactinomyxon eiseniellae les études ultrastructurales montrent que l'enveloppe du pansporocyste, d'une part, les épispores et les capsules polaires d'autre part sont réalisées à partir de cellules distinctes et profondément modifiées. Quant au sporoplasme, autrefois décrit comme un plasmode avec de nombreuses paires de noyaux, il contient, en fait, des ensembles identiques dont chacun est constitué de l'union d'un noyau satellite et d'une cellule uninucléée.〈section xml:id="abs1-2"〉〈title type="main"〉ABSTRACTThe study of the planktonic character of different Actinomyxidia spores reveals increasingly complex adaptations to an aquatic environment. On contact with water, the three episporal cells of each spore transform into floats, the forms of which differ according to species. These floats can join together so that a fourth type of float is formed, or they can unite in various ways the eight spores originating from the same pansporocyst. This is the case in the genus Synactinomyxon whose diagnosis is modified to include a second species S. Iongicauda n. sp. A new type is described in which the presence of anchors at the extremities of the episporal cells permits several dozen spores that have been emitted simultaneously to be kept together. We have observed the emission of the sporoplasm in the genera Aurantiactinomyxon, Synactinomyxon, and Echinactinomyxon. It is freed completely and for more than an hour is capable of changing its position in the water by amoeboid movements. In the case of Aurantiactinomyxon eiseniellae, ultrastructural studies show that the pansporocyst envelope on the one hand, and the epispores and polar capsules on the other hand, are formed from separate but profoundly modified cells. The sporoplasm, however, sometimes described as a plasmodium with numerous pairs of nuclei, contains, in fact, identical complexes, each consisting of a uninucleate cell united with a satellite nucleus.

Notes: Paramecia detect and accumulate in or disperse from some chemicals. Cells do this by changing frequency of turning and speed of swimming. There are at least two mechanisms by which cells respond: one dependent on ability to turn, one dependent on speed modulation. There are also two classes of chemicals: those that require the cells' ability to turn in order to cause accumulation and dispersal (type I), and those that apparently require only speed modulation (type II). Attractants of type I cause qualitatively similar changes in behavior to repellents of type II and the converse; therefore, assays are needed to distinguish between these two classes of chemicals, despite qualitatively similar behavior of some attractants and repellents. We examined two assays of paramecium chemoresponse, T-maze assay and well test, to understand how the T-maze distinguishes between attractants of type I and repellents of type II and why the well test does not.

Notes: The morphology of spore germination in Didymium nigripes was studied using scanning electron microscopy and Nomarski phase optics. First, the outer spore wall splits, revealing a fibrillar inner wall. Remnants of the inner wall continue to cover the newly emerged amoeba. A single nucleus and a prominent vacuole are visible throughout germination. Germination is more rapid in glucose-peptone-yeast extract than in phosphate buffer. Germination is completely inhibited at 4°C, and is very slow at 18°C. Germination is most rapid at 26°C; at 21°C or 32°C it is slightly slower. Germination is reversibly inhibited by 20 μ/ml cycloheximide, but not by 200 μ/ml 5-fluoro uracil or 200 μ/ml proflavin. It is completely inhibited by 10-3 M Na azide.

Notes: Crithidia fasciculata (Anopheles, Culex, and Nöller strains), C. hutneri, C. luciliae thermophila, and Herpetomonas samuelpessoai were grown in a defined medium with different values of osmolarity at different temperatures. C. fasciculata (all strains) grew best between 300 to 500 mOsm; H. samuelpessoai, 400–500 mOsm; and C. hutneri and C. luciliae thermophila, 500–800 mOsm. At higher temperatures better growth was obtained at the upper osmolarities.