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  • Light and electron microscopy  (22)
  • Springer  (22)
  • Cambridge University Press
  • Wiley
  • 1980-1984
  • 1970-1974  (22)
  • 1965-1969
  • 1955-1959
  • 1974  (15)
  • 1972  (7)
Collection
Publisher
  • Springer  (22)
  • Cambridge University Press
  • Wiley
Years
  • 1980-1984
  • 1970-1974  (22)
  • 1965-1969
  • 1955-1959
Year
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 131 (1972), S. 329-346 
    ISSN: 1432-0878
    Keywords: Olfactory mucosa ; Mouse ; Glands of Bowman ; Light and electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Das erste Auftreten der Glandulae olfactoriae in der olfaktorischen Region der Maus wird beschrieben. Die Struktur der Bowmanschen Drüse und ihre topologische Beziehung zu den übrigen zellulären Elementen im Riechepithel wird untersucht. Die Bedeutung des Sekrets für die Bildung des Deckhäutchens wird diskutiert. Die Bowmanschen Drüsen der Maus erreichen im letzten Viertel des intrauterinen Lebens die Lamina propria des Riechepithels. In den Endstücken finden sich dunkle, sekretreiche und helle, sekretarme Zellen. Die sezernierenden Zellen der Bowmanschen Drüsen sind nicht auf die Lamina propria beschränkt, sondern erstrecken sich bis in die untersten Anteile des Kernlagers im Riechepithel. Beim Austritt der Bowmanschen Drüsen aus dem Riechepithel in die Lamina propria konfluieren die Basalmembranen dieser Gewebeanteile miteinander. Die gemeinsame Basalmembran kann sich noch eine Strecke weit bis in den normalen Interzellularraum zwischen Drüsen- und Riechepithelanteilen einsenken. In den apikalen Anteilen des Riechepithels wird der Ausführungsgang von den benachbarten Sinnes- und Stützzellen nur durch eine normal breite Interzellularfuge getrennt. Im Ausführungsgang der Bowmanschen Drüse finden sich dunkle und helle auskleidende Zellen. Die durch Desmosomen miteinander verbundenen Epithelzellen der Ausführungsgänge zeigen Zeichen einer Sekretion. Die periphersten Ausläufer des Ausführungsgangepithels erstrecken sich lediglich bis in das Terminalplattenniveau der Stützzellen, mit denen sie sich durch desmosomenartige Kontaktzonen verbinden. In der Verlängerung der Ausführungsgänge wird das Lumen peripher des Terminalplattenniveaus von den apikalen Stützzellanteilen und deren Mikrovilli sowie von den obersten Anteilen der Dendriten, von den Riechköpfen und den Sinneshaaren der Rezeptorzellen umgrenzt. Im Lumen der Ausführungsgangverlängerung finden sich im Bereich des olfaktorischen Saumes flächenhafte Ansammlungen von Sekret. Das Sekret im intervillösen Raum des olfaktorischen Saumes zeigt eine besondere Affinität zu den Membranen der peripheren Sinneszellausläufer. In der intrauterinen Lebensphase ließ sich bisher kein Deckhäutchen feststellen. Die austrocknende Wirkung der Luft auf das Sekret der Bowmanschen Drüsen wird als Entstehungsmechanismus für das Deckhäutchen in Erwägung gezogen.
    Notes: Summary First appearance, structure and topological relations of Bowmans' glands in the regio olfactoria of white mice are described. The importance of these glands for the formation of the terminal mucous cover of the olfactory epithelium is discussed. In the last quarter of intrauterine life the glands of Bowman reach the lamina propria. In the terminal portion of the glands dark cells with many secretory droplets and pale cells with only a few of them can be seen. Secretory active cells are localized in the basal part of the olfactory epithelium as well. When entering the lamina propria the irregular wide basement membrane of the glands joins that one of the epithelium. It is possible to follow up this joined basement membrane for a short distance between the glands and the cells of the olfactory epithelium. Peripheral to the very basal part of the olfactory epithelium there is no basement membrane around the glands' tissue. Receptors and sustentacular cells are separated from the gland only by a normal intercellular space. The epithelium of the ducts consists of dark and light cells as well. They are connected with the sustentacular cells by desmosomelike contacts. In its prolongation the lumen of Bowman's duct is lined by the apical portions of the sustentacular cells and their microvilli, and by dendrites, olfactory vesicles, and sensory cilia of the receptor cells. In the region of cilia and microvilli one can see masses of secretion which have confluenced. In the intervillous space its special affinity to the receptor's membranes is evident. During the intrauterine phase of life no terminal mucous cover could be demonstrated. The drying effect of the air as a possible reason for the origin of the terminal mucous cover is discussed.
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 132 (1972), S. 245-256 
    ISSN: 1432-0878
    Keywords: Olfactory organs (Fish)-Labyrinth Cells ; Ion transport ; Evolution ; Light and electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Light microscopy and transverse electron microscopy has been employed to study the olfactory organs in 82 specimens of freshwater adapted young and homing adult Baltic sea trout Salmo trutta trutta L. In both sensory and indifferent epithelium the olfactory mucosa has scattered cells of a type that has not been described in any olfactory organ before. They are called labyrinth cells and are characterized by an extensive, turtuous, interconnected tubular system of smooth endoplasmatic reticulum intimately connected with numerous mitochondria. This cell type is similar to chloride and other cells which probably are involved in electrolyte transport in fish gills and pseudobranch, the rectal gland in elasmobranchs and the nasal gland in reptiles and birds. It is suggested that the olfactory organ in fish is serially homologous with the pseudobranch.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 151 (1974), S. 369-384 
    ISSN: 1432-0878
    Keywords: Giant dopamine neurone ; Planorbis corneus ; Localization of dopamine ; Axon tracing ; Light and electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary There is a giant dopamine-containing cell (GDC) in the left pedal ganglion of Planorbis corneus. Some presynaptic endings of the GDC are located within the visceral and left parietal ganglia, other endings are located peripherally. Dense-cored vesicles of 50–250 nm diameter were observed in the perikaryon and primary axon of the GDC. Electron microscope histochemistry suggests that these vesicles contain dopamine. Vesicles with a similar appearance are present in some axonal processes located in areas of the nervous system known to contain presynaptic endings of the GDC. This neurone offers unique advantages for studying the role of neuronal dopamine.
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 151 (1974), S. 489-498 
    ISSN: 1432-0878
    Keywords: Limbic system ; Cat ; Amygdaloid body ; Light and electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The present study was undertaken to re-examine the central nucleus of the cat in Nissl stained sections, to describe its fine structural characteristics, and to compare the lateral subdivision of this nucleus with the putamen. In Nissl preparations, it was observed that the lateral subdivision of the central nucleus contains a fairly homogeneous population of small cells while the putamen contains both small and large cells. It is the presence of a few large cells at the lateral periphery of the lateral part of the central nucleus that makes the boundary between the two nuclei indistinct. Examination of the fine structure of the medial and lateral subdivisions of the central nucleus revealed the presence of many boutons containing flattened vesicles and fewer with spheroid vesicles. In contrast, most of the boutons in the putamen have spheroid vesicles. It is concluded that the lateral subdivision of the central nucleus may be distinguished from the putamen, except at its most lateral border, by its homogeneous population of small cells and its many boutons containing flattened vesicles.
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 153 (1974), S. 219-226 
    ISSN: 1432-0878
    Keywords: Cerebellar cortex ; Man and other mammals ; Golgi cells ; Regional differences ; Light and electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The number of Golgi cells per unit volume was determined in different regions of the cerebellar cortex of man and of ten other mammals. Despite the general belief in the uniform architecture of the cerebellar cortex, regional differences in the distribution of Golgi cells were found. In the inferior parts of the vermis, the number of Golgi cells per unit volume is twice that in the corresponding hemispheres. In addition, there are differences between the anterior and inferior parts of the vermis. These differences are a feature of the cytoarchitecture of the cerebellum in man and all the investigated mammals. The ratio of Purkinje cells to Golgi cells was also determined and found to differ in different species. In man, this ratio is 1∶1.5, while in the monkey and cat it is almost 1∶1.9 and in the rat 1∶3.3. These differences in the ratio of Purkinje cells to Golgi cells are discussed from the point of view of cerebellar evolution.
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 153 (1974), S. 383-398 
    ISSN: 1432-0878
    Keywords: Aesthete Organs ; Lepidochitona cinereus ; Cell types ; Secretory cells ; Light and electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The structure of the aesthete organs of the chiton Lepidochitona cinereus (L.) is described by light and electron microscopy. The observations do not discount a sensory function for these organs, but possible sensory structures such as microvilli and cilia are poorly organised. Most of the aesthete is taken up with large cells actively forming secretory products and apparently passing them proximally in the aesthete canal towards the animal. Comparisons are drawn with the brachiopod caecae.
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 153 (1974), S. 399-413 
    ISSN: 1432-0878
    Keywords: Spinal ganglia (Rat) ; Cell division ; Autoradiography ; Neurone morphogenesis ; Light and electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Pulse labelling with tritiated thymidine was used to determine the time of the final division of the neuroblasts which subsequently form rat lumbar dorsal root ganglion neurones. The final division occurred during a 4 day period, the maximum frequency being on day 12 of gestation. Separation of the ganglion cells into large light neurones and small dark neurones showed that the large light neurones were formed earlier than the small dark neurones. In both cases the final divisions occurred over a period of 3–4 days, but the peak rate of formation of large neurones was on day 12, and that of the small neurones was on day 13. Low power electron micrographs were used to measure mean cell diameter throughout development from day 11 of gestation until a postnatal age of 225 days. A marked increase in cell diameter occurred on day 15–15.5, about 3 days after the final cell divisions of the majority of the cells. The rate of growth increased just before birth, but no increase in mean cell diameter was found between day 21 of gestation and the third day postnatal. The growth was again rapid after this period until a plateau in cell diameter was reached about 33 days after birth.
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 155 (1974), S. 291-320 
    ISSN: 1432-0878
    Keywords: Adenohypophysis ; Chicken ; Developmental endocrinology ; Epithelial differentiation ; Light and electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Anlagen of the pars distalis (Rathke's pouch), adjacent ectoderm, endoderm, infundibulum and mesenchyme left in situ were put in 3% glutaraldehyde between 1–3 pm and fixed overnight. Epon sections of this material from six White Leghorn, Gallus gallus, embryos fixed at each stage, and of two control partes distales from laying hens of the same flock were examined. At stage 17 (∼12 hours after formation) Rathke's pouch cells were stratified, uninnervated, non-vascularized and stellate, with high nucleo-cytoplasmic ratios and few organelles. Except for lipid inclusions, pouch cells did not appear appreciably different by stage 27, either regionally within the pouch wall or from the adjacent epithelioid cells. Apparent major changes indicative of cytoplasmic maturation by stage 27 included: reduction in number of polysomes; appearance of single-membraned, dumb-bell shaped to rounded, dense granula which were usually in basal position but also in areas of the Golgi apparatus; greater prominence of rough endoplasmic reticulum with cisternae containing dense material, and of the Golgi apparatus, notably with variously dense and/or coated vesicles and material; reduction in lipid inclusions by stage 24. Non-terminating axons in the infundibulum were first seen at stage 27. We found no convincing evidence for any possible morphogenetic or other relationship between the pars distalis and surrounding tissues. “Melanophore-stimulating hormone-activity”, reported to appear on day 5 of incubation, “stored thyroid-stimulating hormone” or pars distalis hormone granula or activities could not be identified. Rathke's pouch and other epithelioid structures may have been secreting, taking up nutrients and/or differentiating, activities which could be important for yolk sac development, nutrition and/or cytodifferentiation, respectively.
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 151 (1974), S. 509-523 
    ISSN: 1432-0878
    Keywords: Gill ; Trout ; Secondary lamellae ; Development ; Light and electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A light and electron microscope study of secondary lamellae of trout developing at 10° C is described. Collagen is secreted by mesenchyme cells in the connective tissue of the developing gill filament. This becomes enclosed in infoldings around the peripheries of mesenchyme cells. These cells become aligned in a single plane within folds of basement membrane and epithelium. The basement membrane of opposite sides of the fold becomes connected by a thick layer of collagen. Blood spaces form around the margin of the fold, connecting afferent and efferent filament blood vessels. Endothelial granules form in cells lining the outer border of the marginal channel when blood flow begins. Rows of pillar cells separate from the proximal layer of mesenchyme cells as the secondary lamellae develops further. New secondary lamellae are added at the filament tips. At 28 days, the basement membrane consists of 2 layers, a fine fibrous layer and 5–15 orthogonally arranged layers of collagen fibres. By 31 days, the collagen is arranged at random and the layer is thinner. A clear layer is also present by 67 days. A multilayered epithelium containing mucous and chloride cells is present at 28 days. By 102 days, the secondary lamellae are covered by 2 layers of epithelial cells only. Chloride cells are present in much greater numbers in developing gills than in the adult.
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 155 (1974), S. 449-453 
    ISSN: 1432-0878
    Keywords: Coronet cells ; Teleostei ; Neurohypophysis ; Hypersalinity ; Light and electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In several adult specimens of Mugil capito caught in a hypersaline lagoon, an intra-neurohypophyseal channel system lined with coronet cells has been observed. Coronet cells are normally found only in the saccus vasculosus, and in modern Teleostei, this structure is always completely separated from the neurohypophysis. The penetration of coronet cells into the neural lobe, may be an adaptive phenomenon possibly connected with an osmo-protective role of these cells.
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