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  • Histochemistry  (40)
  • Spektralphotometrie  (35)
  • Cell types
  • Springer  (89)
  • Cambridge University Press
  • 1980-1984
  • 1970-1974  (89)
  • 1965-1969
  • 1955-1959
  • 1973  (42)
  • 1972  (47)
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  • Springer  (89)
  • Cambridge University Press
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  • 1980-1984
  • 1970-1974  (89)
  • 1965-1969
  • 1955-1959
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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Calcified tissue international 12 (1973), S. 169-173 
    ISSN: 1432-0827
    Keywords: Calcification ; Histochemistry ; Autoradiography ; Fetal ; Mandible
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Description / Table of Contents: Résumé Des rats femelles reçoivent du45Ca ou du32P au 13ème et 18ème jour de la grossesse. Les coupes sériées des têtes de foetus sont étudiées par autoradiographie et par diverses méthodes histologiques pour déterminer la calcification. La détection la plus précoce de45Ca s'observe simultanément comme une réaction positive pour le calcium avec une des méthodes histologiques utilisées.32P est en évidence par les méthodes autoradiographiques un peu plus tard que le45Ca et sa présence coincide avec la réaction positive la plus précoce observée avec les autres méthodes histologiques utilisées. Les os de ces têtes foetales commencent à se calcifier selon un mode particulier commençant par la mandibule, puis l'os frontal et le maxillaire supérieur, suivis par les os nasaux, pariétaux et interpariétaux.
    Abstract: Zusammenfassung Weibliche Ratten erhielten45Ca und32P zwischen dem 13.–18. Tag der Schwangerschaft. Zur Ermittlung der Verkalkung wurden Serienschnitte der Köpfe ihrer Feten mittels Autoradiographie sowie durch verschiedene histologische Methoden untersucht. Das erste Auftreten von45Ca war ebenfalls von einer positiven Calciumreaktion mittels einer der verwendeten histologischen Methoden begleitet.32P wurde in der Autoradiographie erst etwas später als45Ca festgestellt und dessen Nachweis deckte sich mit den ersten positiven Reaktonen aller anderen verwendeten histologischen Methoden. Die Knochen in diesen Fetusköpfen begannen in einer bestimmten Sequenz zu verkalken: zuerst der Unterkiefer, dann das Stirnbein und der Oberkiefer, dann das Nasenbein, die Parietal- und Interparietalknochen.
    Notes: Abstract Female rats were given45Ca or32P from 13 to 18 days of pegnancy. Serial sections from the heads of their fetuses were studied by autoradiography, as well as by several histological methods for assessing calcification. The earliest detection of45calcium occured at the same time as a positive reaction for calcium with one of the histological methods used.32P was not detectable by autoradiographic methods until somewhat later than45Ca, and its presence coincided with the earliest positive reaction with all of the other histological methods employed. The bones in these fetal heads began to calcify in a partcular sequence, the mandible first, then the frontal bone and maxilla, followed by the nasal, parietal and interparietal bones.
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Calcified tissue international 10 (1972), S. 49-57 
    ISSN: 1432-0827
    Keywords: Histochemistry ; Cartilage ; Enzymes ; Vitamin D ; Starvation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Description / Table of Contents: Résumé 1. La distribution quantitative de phosphatase alcaline et de déshydrogénas eglucose-6-phosphate dans les zones hypertrophiques, en voie de prolifération du cartilage de rats rachitiques, pendant la cicatrisation induite par l'administration de vitamin D ou par jeun prolongé, est étudiée. 2. L'activité en phosphatase alcaline de la zone hypertrophique est environ triple de celle de la zone de prolifération du rat sevré normal et environ quadruple de celle des rats rachitiques. 3. Apres administration d'une seule dose de vitamine D (8000 unités), il se produit une augmentation significative de l'activité en phosphatase alcaline de la a zone en voie de prolifération. Une augmentation moins nette est observée dans la zone de prolifération de rats ayant jeuné 48 heures. 4. On observe une diminution significative de l'activité en phosphatase alcaline de la partie centrale de la zone hypertrophique de cartilage aprés traitement a la vitamine D. Une telle diminution n'a pas été observée chez le rat qui jeûne. 5. Chez le rat sevré normal, l'activité en déshydrogénase glucose-6-phosphate (G-6-PDH) est plus élevée dans la zone de prolifération que dans la zone hypertrophique. Chez le rat rachitique non traité, le contenu en enzyme est à peu pres identique dans les deux zones. 6. Apres traitement a la vitamine D, on note une décroissance nette de l'activité en G-6-PDH. Une telle décroissance n'est pas observée chez le rat qui jeûne.
    Abstract: Zusammenfassung 1. Diese Arbeit beschreibt die quantitative Verteilung der alkalischen Phosphatase und der Glucose-6-Phosphat-Dehydrogenase in proliferierenden und hypertrophischen Zonen von rachitischem Rattenknorpel während der durch Vitamin D-Gabe hervorgerufenen Heilung oder während der Mangeldiät. 2. Die Aktivität der alkalischen Phosphatase in der hypertrophischen Zone war etwa dreimal so groß wie in der proliferierende Zone der normalen entwöhnten Ratte und etwa viermal so groß bei den rachitischen Ratten. 3. Nach einer einmaligen Vitamin D-Gabe (8000 i.E) erfolgte eine signifikante Erhöhung der alkalischen Phosphatasen-Aktivität in der proliferierenden Zone. Eine weniger signifikante Erhöhung wurde in der proliferierenden Zone von Ratten festgestellt, welche 48 Std gefastet hatten. 4. Nach Vitamin D-Behandlung erfolgte eine signifikante Abnahme der alkalischen Phosphatasen-Aktivität in der mittleren Region der hypertrophischen Zone des Knorpels. Bei den fastenden Ratten wurde keine solche Abnahme beobachtet. 5. Bei der normalen entwöhnten Ratte war die Aktivität der Glucose-6-Phosphat-Dehydrogenase (G-6-PDH) größer in der proliferierenden als in der hypertrophischen Zone. Bei der unbehandelten rachitischen Ratte war der Enzymgehalt in beiden Zonen etwa gleich. 6. Nach Vitamin D-Behandlung erfolgte eine merkliche Abnahme der G-6-PDH-Aktivität. Bei der fastenden Ratte wurde keine solche Abnahme beobachtet.
    Notes: Abstract 1. The quantitative distribution of alkaline phosphatase and glucose-6-phosphate dehydrogenase in proliferating and hypertrophic zones of rachitic rat cartilage during healing induced by vitamin D administration or starvation is presented. 2. Alkaline phosphatase activity in the hypertrophic zone is about three times greater than that of the proliferating zone of the normal weanling rat and approximately four times greater in the rachitic rats. 3. Following a single dose of vitamin D (8000 I. U.) there was a significant elevation in alkaline phosphatase activity of the proliferating zone. A less significant increase was observed in the proliferating zone of rats which had breen fasted 48 hours. 4. There was a significant decrease in the alkaline phosphatase activity of the central region of hypertrophic zone of cartilage after vitamin D treatment. No such decrease was observed in the fasted rats. 5. In the normal weanling rat glucose-6-phosphate dehydrogenase (G-6-PDH) activity was greater in the proliferating zone as compared to the hypertrophic zone. In the untreated rachitic rat the enzyme content was about the same in the two zones. 6. Following vitamin D treatment there was an appreciable decrease in the G-6-PDH activity. No such decrease was observed in the fasted rat.
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 126 (1972), S. 261-277 
    ISSN: 1432-0878
    Keywords: Adrenal medulla ; Rat ; Cell types ; Secretion ; Influence of fixation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Das Nebennierenmark der Ratte wurde nach Anwendung verschiedener Fixationsmethoden untersucht. Nach Immersionsfixation mit Glutaraldehyd oder Osmiumtetroxyd finden sich in der Nebenniere sog. Mischzellen, helle Zellen, syncytiale oder plasmodiale Zellen, die von den Untersuchern als Artefaktbildungen angesehen werden. In allen Proben nach Perfusionsfixation wurden solche Artefaktbildungen niemals beobachtet. Bei Immersionsfixation wurde eine enge Beziehung zwischen Sekretgranula und Cytoplasmamembran beobachtet, was bei Perfusionsfixation selten der Fall war. Die heutigen Theorien über den Sekretionsmechanismus bei Nebennierenmarkzellen werden aufgrund der vorgelegten Ergebnisse diskutiert. Die Arbeit zeigt die wesentlichen Vorteile der Anwendung der Perfusionsfixation für die Untersuchung der Nebenniere.
    Notes: Summary The adrenal medulla of the rat was studied utilizing various methods of fixation. In adrenal medulla specimens after immersion fixation either with glutaraldehyde or osmium tetroxide, elements such as mixed, clear, syncytial, or plasmodial cells, believed to be of artifactual origin, are observed in all of this material examined. These elements are absent in the specimens prepared by perfusion fixation. In specimens prepared by immersion fixation, secretory granules are found in close proximity to the plasma membrane; this localization is infrequent after perfusion fixation. Current theories of the mechanism of secretion of adrenal medullary hormones are discussed on the basis of our results. This investigation demonstrates the advantage and necessity of perfusion fixation in the study of the adrenal medulla.
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 126 (1972), S. 335-347 
    ISSN: 1432-0878
    Keywords: Glandulae submaxillares ; Mammals ; Duct cell granules ; Ultrastructure ; Histochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Discrete, PAS-positive granules of relatively uniform electron-density and size characterise the intercalated duct cells of mammalian submaxillary glands. Smaller, electron-dense organelles are seen in the cells at the junction of the intercalary-striated duct region in the guinea-pig. The large granules of variable electron-density which are observed in the proximal, modified intercalary cells in the rabbit closely resemble the granules in the acinar cells of the guinea-pig. Several populations of granules differing in size are found in the striated granular tubules of the rat and hamster; the organelles in the rat show two grades of electron-density whereas those in the hamster are uniformly dense. Numerous small granules with compactly arranged intragranular material occupy the apical part of the striated ducts of the cat, dog and rabbit. The chemical composition of each population of duct cell granules is unknown. The question whether granules containing kallikrein, trypsin-like enzymes and amylase are stored in the duct cells is discussed.
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 131 (1972), S. 47-62 
    ISSN: 1432-0878
    Keywords: Neurohypophysis ; Lungfish ; Ultrastructure ; Histochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The neurohypophysis of the South American lungfish Lepidosiren paradoxa has been studied with light and electron microscopy, including the Falck-Hillarp technique for catecholamines. The pars nervosa hypophyseos is a well-marked, dorsally located subdivision of the pituitary gland composed of lobes or follicles, each one constituted of a central core of ependymal cells, a subependymal hilar region made up of nerve fibers and a peripheric palisade zone of nerve endings which contact capillary vessels. Four types of neurosecretory axons can be distinguished under the electron microscope. Type I, the most common, contains spherical elementary granules of high electron density, 1500–1800 Å in diameter. The scarce type II axons contain irregularly-shaped elementary granules. Type III contains only small clear vesicles, 400–600 Å in diameter. Type IV, mostly present in regions of the gland contacting the pars intermedia, contain large granulated vesicles, 900–1000 Å in diameter. The Falck-Hillarp technique revealed axons with a positive reaction for catecholamines at sites corresponding approximately to the location of type IV of the electron microscope. Ependymal cells are of large size, linking the cerebrospinal fluid, the nerve endings and the blood vessels. A conspicuous membrane-bound, spherical dense material, 1400–2000 Å in diameter, is observed in both the apical and vascular processes of these cells. The ependymal processes which traverse the hilar and palisade regions contain structures resembling degenerated neurosecretory axons. These results are discussed in relation with the currently available information on the comparative anatomy of the pars nervosa. The possible functional significance of ependymal cells and of each type of axon are also discussed. This study was aided by the following grants: NIH NS 06953 to Prof. De Robertis, Consejo Nacional de Investigaciones Científicas y Técnicas to Prof. Zambrano, Comisión de Investigaciones Científicas de la Provincia de Buenos Aires and Comisión de Investigaciones Cientificas de la Universidad Nacional de la Plata: to Prof. Iturriza. The authors are indebted to Prof. De Robertis for his generosity in granting us his laboratory facilities, and to Dr. F. J. J. Risso and Mr. A. Fernández (Resistencia, Chaco) who provided the specimens used in this study. The able microtechnical assistance of Miss L. Riboldazzi and Mrs. R. Raña and the photographic work of Mr. A. Saenz are much appreciated.
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 131 (1972), S. 299-308 
    ISSN: 1432-0878
    Keywords: Testis, rat ; Myoid cells ; Androgens ; Histochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary To study the regulation of the development of myoid cells, seminiferous tubules of adult and prepubertal rats were grown in organ culture under the influence of testosterone, HCG and cyproterone acetate. Contractility, EM-structure and histochemical activities of alkaline phosphatase and ATPase of the myoid cells were studied in the adult rat tubules at one week intervals up to 5 weeks in culture, in the prepubertal rat tubules at the age of 15 days and after 15 and 21 days in culture. As in vivo, contractions appear in cultured tubules of prepubertal rats at the age of 15 days. Testosterone and HCG increase the percentage of contractile tubules and the number of filaments of the myoid cells. Cyproterone acetate inhibits both functional and structural development and tends to decrease the enzyme activities. In the cultured adult rat tubules cyproterone acetate causes disappearance of contractility within one week, while contractions normally are found for 3 weeks. Testosterone and HCG have no notable effects on the contractility of adult rat tubules, but they lengthen the persistence of alkaline phosphatase activity. It is concluded that the maturation and also the functioning of the myoid cells are subject to androgenic regulation.
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 131 (1972), S. 519-528 
    ISSN: 1432-0878
    Keywords: Cardiac innervation ; Teleosts ; Acetylcholinesterase ; Fine structure ; Histochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The innervation of the heart of the plaice has been studied ultrastructurally and histochemically in order to describe the anatomy, and the nature of the neuro-transmitters involved in the regulation of the heart. A single cardiac branch of the vagus runs over the Duct of Cuvier and sinus venosus where it forms a plexus extending into the sinoatrial region. Many ganglion cells are scattered throughout this plexus upon which are seen cholinergic-type terminals at which AChE can be localised. It is therefore concluded that this is the parasympathetic cardiac ganglion. The innervation of the atrium is dense at the sinoatrial end but decreases towards the ventricle. The ventricle is probably aneural, possibly a consequence of the lack of a coronary blood supply to act as a pathway for an innervation of this chamber. Most of the intramural axon profiles contain large numbers of small agranular vesicles and are cholinergic, AChE being localised at their membranes. Other profiles contain small numbers of larger granular vesicles amongst the agranular vesicles but these are not adrenergic since these vesicles are not depleted by reserpine, do not load with 6-OHDA, and AChE can be localised at the membranes of the profiles. No adrenergic-type profiles were seen in any part of the heart, nor were any fluorescent, catecholamine-containing fibres observed.
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 132 (1972), S. 451-472 
    ISSN: 1432-0878
    Keywords: Adenohypophysis ; Myxine glutinosa ; Cell types ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The ultrastructure of adenohypophysial cells in the Atlantic hagfish (Myxine glutinosa) is described and the morphological evidence for secretory activity is discussed. A scarcity of secretory granules is characteristic of the adenohypophysis of Myxine. Two cell types having the appearance of protein hormone producing cells can be identified. Type 1 has dense membrane-bound granules with a calculated mean diameter of 88 nm while type 2 has larger granules with a mean diameter of 176 nm. The release of secretory granular material follows mainly the “membrane-release” pattern. It is suggested that cell type 1 may produce a hormone which is similar to ACTH/MSH and type 2 another hormone similar to STH/LTH. The basophilic cells contain a secretory material which is similar to the mucus produced in the epithelial mucus cells. Several structural modifications are considered to represent functional compensations for the absence of vascular elements in the gland. Among these are a cytoplasmic tubular system, certain long agranular cells together with long granule-containing projections from cell types 1 and 2, and foliate or finger-like invaginations of the basal lamina.
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 130 (1972), S. 338-350 
    ISSN: 1432-0878
    Keywords: Adenohypophysis ; Salmon ; Gonad maturation ; Cell types ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The fine structure of the various hormone-producing cell types (with the exclusion of the prolactin cells) in the pituitary gland (pars distalis) of migratory sockeye salmon is described. All fish were in an advanced stage of sexual maturation. In the proximal pars distalis five cell types were distinguished: growth hormone cells, ACTH cells, gonadotrops, “vesicular cells”, and “chromophobe cells”. Gonadotrops were also found throughout the rostral pars distalis. A conspicuous feature of the gonadotrops was the presence of two kinds of secretory inclusions: small electron-dense granules (200–375 mμ) and large, relatively electron-translucent globules (400–2 000 mμ). The large vesicular cells, so called because of their conspicuous vesicular endoplasmic reticulum, were numerous and often appeared to contain some small granules. It is argued that they may represent a second type of gonadotropic cell, which, in earlier stages of gonad development, contains many granules but becomes largely degranulated near the time of reproduction when the other gonadotrops (“globular gonadotrops”) abound. The chromophobes, which were smaller and far less abundant than the vesicular cells, also appeared to contain small granules (120–280 mμ). They are probably thyrotrops.
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  • 10
    ISSN: 1432-0878
    Keywords: Ultimobranchial glands ; Urodeles ; Cell types ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The fine structure of the ultimobranchial (UB) glands of two common laboratory urodeles, viz., larval axolotls, Ambystoma mexicanum Shaw and adult Pleurodeles waltlii Micahelles, is described and compared in what is believed to be the first ultrastructural report on urodele UB glands. The axolotl UB gland shows a wide variety of form, being represented by an elongated diffuse series of follicles and sometimes by one or two large discrete terminal follicular bodies. In these axolotl UB glands up to four cell categories are distinguishable including a tonofilamentous cell and a secretory cell that is possibly homologous with calcitonin-producing C cells of anurans or other vertebrates. These two cell categories are also found in the Pleurodeles gland. The possible significance of the various cells is considered.
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