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  • Articles  (40)
  • Histochemistry  (40)
  • Springer  (40)
  • 2020-2020
  • 1970-1974  (40)
  • 1945-1949
  • 1973  (18)
  • 1972  (22)
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  • Articles  (40)
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  • Springer  (40)
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  • 2020-2020
  • 1970-1974  (40)
  • 1945-1949
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  • 1
    Electronic Resource
    Electronic Resource
    A comparison of histological methods for demonstrating calcification (1973)
    Springer
    Calcified tissue international 12 (1973), S. 169-173 
    Details
    ISSN: 1432-0827
    Keywords: Calcification ; Histochemistry ; Autoradiography ; Fetal ; Mandible
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Description / Table of Contents: Résumé Des rats femelles reçoivent du45Ca ou du32P au 13ème et 18ème jour de la grossesse. Les coupes sériées des têtes de foetus sont étudiées par autoradiographie et par diverses méthodes histologiques pour déterminer la calcification. La détection la plus précoce de45Ca s'observe simultanément comme une réaction positive pour le calcium avec une des méthodes histologiques utilisées.32P est en évidence par les méthodes autoradiographiques un peu plus tard que le45Ca et sa présence coincide avec la réaction positive la plus précoce observée avec les autres méthodes histologiques utilisées. Les os de ces têtes foetales commencent à se calcifier selon un mode particulier commençant par la mandibule, puis l'os frontal et le maxillaire supérieur, suivis par les os nasaux, pariétaux et interpariétaux.
    Abstract: Zusammenfassung Weibliche Ratten erhielten45Ca und32P zwischen dem 13.–18. Tag der Schwangerschaft. Zur Ermittlung der Verkalkung wurden Serienschnitte der Köpfe ihrer Feten mittels Autoradiographie sowie durch verschiedene histologische Methoden untersucht. Das erste Auftreten von45Ca war ebenfalls von einer positiven Calciumreaktion mittels einer der verwendeten histologischen Methoden begleitet.32P wurde in der Autoradiographie erst etwas später als45Ca festgestellt und dessen Nachweis deckte sich mit den ersten positiven Reaktonen aller anderen verwendeten histologischen Methoden. Die Knochen in diesen Fetusköpfen begannen in einer bestimmten Sequenz zu verkalken: zuerst der Unterkiefer, dann das Stirnbein und der Oberkiefer, dann das Nasenbein, die Parietal- und Interparietalknochen.
    Notes: Abstract Female rats were given45Ca or32P from 13 to 18 days of pegnancy. Serial sections from the heads of their fetuses were studied by autoradiography, as well as by several histological methods for assessing calcification. The earliest detection of45calcium occured at the same time as a positive reaction for calcium with one of the histological methods used.32P was not detectable by autoradiographic methods until somewhat later than45Ca, and its presence coincided with the earliest positive reaction with all of the other histological methods employed. The bones in these fetal heads began to calcify in a partcular sequence, the mandible first, then the frontal bone and maxilla, followed by the nasal, parietal and interparietal bones.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02013732
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  • 2
    Electronic Resource
    Electronic Resource
    Quantitative histochemistry of cartilage (1972)
    Springer
    Calcified tissue international 10 (1972), S. 49-57 
    Details
    ISSN: 1432-0827
    Keywords: Histochemistry ; Cartilage ; Enzymes ; Vitamin D ; Starvation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Description / Table of Contents: Résumé 1. La distribution quantitative de phosphatase alcaline et de déshydrogénas eglucose-6-phosphate dans les zones hypertrophiques, en voie de prolifération du cartilage de rats rachitiques, pendant la cicatrisation induite par l'administration de vitamin D ou par jeun prolongé, est étudiée. 2. L'activité en phosphatase alcaline de la zone hypertrophique est environ triple de celle de la zone de prolifération du rat sevré normal et environ quadruple de celle des rats rachitiques. 3. Apres administration d'une seule dose de vitamine D (8000 unités), il se produit une augmentation significative de l'activité en phosphatase alcaline de la a zone en voie de prolifération. Une augmentation moins nette est observée dans la zone de prolifération de rats ayant jeuné 48 heures. 4. On observe une diminution significative de l'activité en phosphatase alcaline de la partie centrale de la zone hypertrophique de cartilage aprés traitement a la vitamine D. Une telle diminution n'a pas été observée chez le rat qui jeûne. 5. Chez le rat sevré normal, l'activité en déshydrogénase glucose-6-phosphate (G-6-PDH) est plus élevée dans la zone de prolifération que dans la zone hypertrophique. Chez le rat rachitique non traité, le contenu en enzyme est à peu pres identique dans les deux zones. 6. Apres traitement a la vitamine D, on note une décroissance nette de l'activité en G-6-PDH. Une telle décroissance n'est pas observée chez le rat qui jeûne.
    Abstract: Zusammenfassung 1. Diese Arbeit beschreibt die quantitative Verteilung der alkalischen Phosphatase und der Glucose-6-Phosphat-Dehydrogenase in proliferierenden und hypertrophischen Zonen von rachitischem Rattenknorpel während der durch Vitamin D-Gabe hervorgerufenen Heilung oder während der Mangeldiät. 2. Die Aktivität der alkalischen Phosphatase in der hypertrophischen Zone war etwa dreimal so groß wie in der proliferierende Zone der normalen entwöhnten Ratte und etwa viermal so groß bei den rachitischen Ratten. 3. Nach einer einmaligen Vitamin D-Gabe (8000 i.E) erfolgte eine signifikante Erhöhung der alkalischen Phosphatasen-Aktivität in der proliferierenden Zone. Eine weniger signifikante Erhöhung wurde in der proliferierenden Zone von Ratten festgestellt, welche 48 Std gefastet hatten. 4. Nach Vitamin D-Behandlung erfolgte eine signifikante Abnahme der alkalischen Phosphatasen-Aktivität in der mittleren Region der hypertrophischen Zone des Knorpels. Bei den fastenden Ratten wurde keine solche Abnahme beobachtet. 5. Bei der normalen entwöhnten Ratte war die Aktivität der Glucose-6-Phosphat-Dehydrogenase (G-6-PDH) größer in der proliferierenden als in der hypertrophischen Zone. Bei der unbehandelten rachitischen Ratte war der Enzymgehalt in beiden Zonen etwa gleich. 6. Nach Vitamin D-Behandlung erfolgte eine merkliche Abnahme der G-6-PDH-Aktivität. Bei der fastenden Ratte wurde keine solche Abnahme beobachtet.
    Notes: Abstract 1. The quantitative distribution of alkaline phosphatase and glucose-6-phosphate dehydrogenase in proliferating and hypertrophic zones of rachitic rat cartilage during healing induced by vitamin D administration or starvation is presented. 2. Alkaline phosphatase activity in the hypertrophic zone is about three times greater than that of the proliferating zone of the normal weanling rat and approximately four times greater in the rachitic rats. 3. Following a single dose of vitamin D (8000 I. U.) there was a significant elevation in alkaline phosphatase activity of the proliferating zone. A less significant increase was observed in the proliferating zone of rats which had breen fasted 48 hours. 4. There was a significant decrease in the alkaline phosphatase activity of the central region of hypertrophic zone of cartilage after vitamin D treatment. No such decrease was observed in the fasted rats. 5. In the normal weanling rat glucose-6-phosphate dehydrogenase (G-6-PDH) activity was greater in the proliferating zone as compared to the hypertrophic zone. In the untreated rachitic rat the enzyme content was about the same in the two zones. 6. Following vitamin D treatment there was an appreciable decrease in the G-6-PDH activity. No such decrease was observed in the fasted rat.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02012535
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  • 3
    Electronic Resource
    Electronic Resource
    II. Ultrastructure of duct cell granules in mammalian submaxillary glands (1972)
    Springer
    Cell & tissue research 126 (1972), S. 335-347 
    Details
    ISSN: 1432-0878
    Keywords: Glandulae submaxillares ; Mammals ; Duct cell granules ; Ultrastructure ; Histochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Discrete, PAS-positive granules of relatively uniform electron-density and size characterise the intercalated duct cells of mammalian submaxillary glands. Smaller, electron-dense organelles are seen in the cells at the junction of the intercalary-striated duct region in the guinea-pig. The large granules of variable electron-density which are observed in the proximal, modified intercalary cells in the rabbit closely resemble the granules in the acinar cells of the guinea-pig. Several populations of granules differing in size are found in the striated granular tubules of the rat and hamster; the organelles in the rat show two grades of electron-density whereas those in the hamster are uniformly dense. Numerous small granules with compactly arranged intragranular material occupy the apical part of the striated ducts of the cat, dog and rabbit. The chemical composition of each population of duct cell granules is unknown. The question whether granules containing kallikrein, trypsin-like enzymes and amylase are stored in the duct cells is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00306848
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  • 4
    Electronic Resource
    Electronic Resource
    Histology and ultrastructure of the neurohypophysis of the south american lungfish, Lepidosiren paradoxa (1972)
    Springer
    Cell & tissue research 131 (1972), S. 47-62 
    Details
    ISSN: 1432-0878
    Keywords: Neurohypophysis ; Lungfish ; Ultrastructure ; Histochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The neurohypophysis of the South American lungfish Lepidosiren paradoxa has been studied with light and electron microscopy, including the Falck-Hillarp technique for catecholamines. The pars nervosa hypophyseos is a well-marked, dorsally located subdivision of the pituitary gland composed of lobes or follicles, each one constituted of a central core of ependymal cells, a subependymal hilar region made up of nerve fibers and a peripheric palisade zone of nerve endings which contact capillary vessels. Four types of neurosecretory axons can be distinguished under the electron microscope. Type I, the most common, contains spherical elementary granules of high electron density, 1500–1800 Å in diameter. The scarce type II axons contain irregularly-shaped elementary granules. Type III contains only small clear vesicles, 400–600 Å in diameter. Type IV, mostly present in regions of the gland contacting the pars intermedia, contain large granulated vesicles, 900–1000 Å in diameter. The Falck-Hillarp technique revealed axons with a positive reaction for catecholamines at sites corresponding approximately to the location of type IV of the electron microscope. Ependymal cells are of large size, linking the cerebrospinal fluid, the nerve endings and the blood vessels. A conspicuous membrane-bound, spherical dense material, 1400–2000 Å in diameter, is observed in both the apical and vascular processes of these cells. The ependymal processes which traverse the hilar and palisade regions contain structures resembling degenerated neurosecretory axons. These results are discussed in relation with the currently available information on the comparative anatomy of the pars nervosa. The possible functional significance of ependymal cells and of each type of axon are also discussed. This study was aided by the following grants: NIH NS 06953 to Prof. De Robertis, Consejo Nacional de Investigaciones Científicas y Técnicas to Prof. Zambrano, Comisión de Investigaciones Científicas de la Provincia de Buenos Aires and Comisión de Investigaciones Cientificas de la Universidad Nacional de la Plata: to Prof. Iturriza. The authors are indebted to Prof. De Robertis for his generosity in granting us his laboratory facilities, and to Dr. F. J. J. Risso and Mr. A. Fernández (Resistencia, Chaco) who provided the specimens used in this study. The able microtechnical assistance of Miss L. Riboldazzi and Mrs. R. Raña and the photographic work of Mr. A. Saenz are much appreciated.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307200
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  • 5
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    Electronic Resource
    Effect of androgens and antiandrogens on the development of the myoid cells of the rat seminiferous tubules (organ culture) (1972)
    Springer
    Cell & tissue research 131 (1972), S. 299-308 
    Details
    ISSN: 1432-0878
    Keywords: Testis, rat ; Myoid cells ; Androgens ; Histochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary To study the regulation of the development of myoid cells, seminiferous tubules of adult and prepubertal rats were grown in organ culture under the influence of testosterone, HCG and cyproterone acetate. Contractility, EM-structure and histochemical activities of alkaline phosphatase and ATPase of the myoid cells were studied in the adult rat tubules at one week intervals up to 5 weeks in culture, in the prepubertal rat tubules at the age of 15 days and after 15 and 21 days in culture. As in vivo, contractions appear in cultured tubules of prepubertal rats at the age of 15 days. Testosterone and HCG increase the percentage of contractile tubules and the number of filaments of the myoid cells. Cyproterone acetate inhibits both functional and structural development and tends to decrease the enzyme activities. In the cultured adult rat tubules cyproterone acetate causes disappearance of contractility within one week, while contractions normally are found for 3 weeks. Testosterone and HCG have no notable effects on the contractility of adult rat tubules, but they lengthen the persistence of alkaline phosphatase activity. It is concluded that the maturation and also the functioning of the myoid cells are subject to androgenic regulation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00582853
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  • 6
    Electronic Resource
    Electronic Resource
    Ultrastructural and histochemical studies on the innervation of the heart of a teleost, pleuronectes platessa L. (1972)
    Springer
    Cell & tissue research 131 (1972), S. 519-528 
    Details
    ISSN: 1432-0878
    Keywords: Cardiac innervation ; Teleosts ; Acetylcholinesterase ; Fine structure ; Histochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The innervation of the heart of the plaice has been studied ultrastructurally and histochemically in order to describe the anatomy, and the nature of the neuro-transmitters involved in the regulation of the heart. A single cardiac branch of the vagus runs over the Duct of Cuvier and sinus venosus where it forms a plexus extending into the sinoatrial region. Many ganglion cells are scattered throughout this plexus upon which are seen cholinergic-type terminals at which AChE can be localised. It is therefore concluded that this is the parasympathetic cardiac ganglion. The innervation of the atrium is dense at the sinoatrial end but decreases towards the ventricle. The ventricle is probably aneural, possibly a consequence of the lack of a coronary blood supply to act as a pathway for an innervation of this chamber. Most of the intramural axon profiles contain large numbers of small agranular vesicles and are cholinergic, AChE being localised at their membranes. Other profiles contain small numbers of larger granular vesicles amongst the agranular vesicles but these are not adrenergic since these vesicles are not depleted by reserpine, do not load with 6-OHDA, and AChE can be localised at the membranes of the profiles. No adrenergic-type profiles were seen in any part of the heart, nor were any fluorescent, catecholamine-containing fibres observed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00306968
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  • 7
    Electronic Resource
    Electronic Resource
    Les cellules sensorielles des papilles de la trompe chez Glycera convoluta Keferstein (Annélide Polychète) (1972)
    Springer
    Cell & tissue research 134 (1972), S. 351-366 
    Details
    ISSN: 1432-0878
    Keywords: Sensory cell ; Proboscis ; Glycera convoluta ; Papilla ; Electronmicroscopy ; Histochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Résumé Chaque papille de la trompe chez Glycera convoluta contient un groupe central de deux à quatre cellules sensorielles primaires. Chaque cellule possède un noyau allongé situé à mi-hauteur de la papille. Le processus péripherique ou dendritique porte cinq à six cils qui traversent la cuticule et sont entourés chacun par un cercle de microvillosités; les cils se prolongent dans le cytoplasme par de fines racines ciliaires qui se réunissent pour former une seule énorme racine montrant une striation périodique. Le processus central ou axonal se prolonge dans le plexus nerveux sous-épithélial. Le rôle physiologique de ces cellules est discuté.
    Notes: Summary Each papilla in the proboscis of Glycera convoluta contains a central group of two to four primary sensory cells. Each one shows an elongated nucleus situated at half-height of the papilla. The peripheric or dendritic process bears five to six cilia traversing the cuticle; each of them is circled by a ring of microvilli. The cilia are prolonged in the cytoplasm by fine ciliary rootlets which gather themselves in a very big single root showing a periodic striation. The central or axonal process runs in the subepithelial nervous plexus. The physiological role of the cells is discussed.
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    URL: http://dx.doi.org/10.1007/BF00307171
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  • 8
    Electronic Resource
    Electronic Resource
    Distribution of acetyl cholinesterase in the hippocampal region of the guinea pig (1972)
    Springer
    Cell & tissue research 131 (1972), S. 481-495 
    Details
    ISSN: 1432-0878
    Keywords: Hippocampal region ; Guinea pig ; AChE distribution pattern ; Histochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary 1. The distribution of acetyl cholinesterase (AChE) has been described in the dentate area, a part of the hippocampal region, in the adult guinea pig. The enzyme was demonstrated histochemically with a modification of the Koelle thiocholine method applied to formaldehyde-fixed frozen sections and unfixed cryostat sections. Non-specific cholinesterase was suppressed by ethopropazine, while the staining reaction for AChE was controlled by complete specific inhibition with BW 284c51. A single brain was stained according to the method of Karnovsky and Roots. 2. The abundant AChE found in the dentate area exhibited a distinctly stratified distribution pattern. In the molecular layer, strong reaction was present in the outer third and immediately above the granular cell layer, the intermediate zone being light. The granular cell bodies were unstained. In the hilus, five layers showing alternating stronger and weaker reaction for AChE were recognizable. 3. In view of the opinions of Cajal, Lorente de Nó, and Blackstad criteria for the definition of the dentate area are discussed. The present results fit into a concept of a layered guinea pig hilus representative of one group of mammals (other members being rabbit, monkey, and man) differing morphologically from the non-layered hilus of rat and mouse. The distribution of metal in the guinea pig hilus supports the concept. 4. Possible structural correlates to the AChE are considered and a comparison with the distribution of AChE in the rat, reported earlier, has been made. In the molecular layer, the most striking difference was the heavy activity observed in the outer third in the guinea pig, where the content is moderate in the rat. The granular cell layer appeared virtually identical in both species. In the hilus the stratified pattern in the guinea pig, contrasting with the more diffuse distribution in the rat, essentially reflects the differing structural architectonics in the hilus of the two species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00306966
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  • 9
    Electronic Resource
    Electronic Resource
    Morphologische und funktionelle Befunde nach Milchsäuredauerinfusion in das Jejunum der Ratte (1972)
    Springer
    Cell & tissue research 132 (1972), S. 107-129 
    Details
    ISSN: 1432-0878
    Keywords: Jejunum (rat) ; Lactic acid perfusion ; Mucosal hypertrophy ; Histology ; Histochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung An weiblichen Wistarratten wurden die zellulären und strukturellen Veränderungen der Dünndarmschleimhaut nach chronischer MS-Belastung durch 12tägige Dauerinfusion ins Jejunum untersucht. Folgende Befunde wurden erhoben: 1. Die MS-Belastung führt in Abhängigkeit von der Konzentration zur Reduktion des Glukosetransports. 2. Die Funktionsstörung ist bedingt durch Enzymaktivitätsverlust. 3. Die Reduktion betrifft alle enzymhistochemisch untersuchten Aktivitäten; sie zeigt, daß alle Zellkompartimente des Epithels beeinträchtigt sind. 4. Qualitative Abweichungen des Reaktionsmusters treten nach MS-Belastung nur beim Saure-Phosphatase-Nachweis auf. Sie sind gekennzeichnet durch Schwund der Markierbarkeit lysosomaler Granula und Auftreten einer schwachen, diffusen cytoplasmatischen Aktivität. 5. Die Schleimhaut unterliegt einer von der MS-Konzentration abhängigen Transformation mit Verschiebung der Zottenhöhen und Kryptentiefen. Sie wird begleitet von einer gesteigerten mitotischen Aktivität als Folge erhöhter Zellexfoliation an der Zottenspitze. Es handelt sich bei dieser Veränderung somit um eine Schleimhauttransformation vom hyper-regeneratorischen Typ. Ihr Initialstadium ist eine Hypertrophie, ihr Endstadium die zottenlose Schleimhaut mit stark veränderten Krypten. Das Endstadium wird unter MS-Belastung nicht ausgebildet, da geringe Konzentrationssteigerungen zu lokaler Gewebsschädigung und Darmperforation führen. 6. Das MS-Modell ist wegen seiner geringen experimentellen Breite ungeeignet für die biochemische Nacharbeitung der bei der einheimischen Sprue beobachteten cytochemischen Epithelveränderungen.
    Notes: Summary Cellular alterations and mucosal changes in zonation of the small intestine have been studied after prolonged perfusion (12 days) with lactic acid in the rat. These investigations were carried out with cytochemical methods using marker enzymes to study in detail the different stages of mucosal transformation of the hyperregenerative type. Results. 1. Lactic acid produces a dose-dependent reduction in glucose absorption and this is due to alterations of the absorptive epithelium. 2. The normal zonation of the small intestinal mucosa is changed. The degree and pattern of the alterations is dose-dependent. Mucosal hypertrophy—i.e. increase of villus height, crypt length and mitotic activity—is the initial stage of this process. 3. Both, cellular alterations and mucosal changes in zonation are the expression of strong compensatory activities in response to predominantly intraluminal stress. The relationship between mucosal hypertrophy after intestinal resection and mucosal transformation of the hyperregenerative type as induced by lactic acid infusion is discussed. 4. In view of its very limited experimental range the lactic acid model cannot be recommended for the study of the epithelial changes in mucosal transformation at a biochemical level.
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    URL: http://dx.doi.org/10.1007/BF00310300
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  • 10
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    Electronic Resource
    A morphologic and histochemical study of biliary atresia in lamprey liver (1973)
    Springer
    Cell & tissue research 136 (1973), S. 85-96 
    Details
    ISSN: 1432-0878
    Keywords: Liver ; Lampetra ; Physiological biliary atresia ; Electron microscopy ; Histochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A morphologic and histochemical study was carried out on the liver of larval and adult lampreys at the optical and electron microscopic level. In the larva the liver is composed of blind ending single cell thick tubules of hepatocytes. The tubular lumina provided with microvilli are morphologically comparable with the canalicular lumens of the higher species of animals. The cytoplasm of the hepatocytes contains numerous inclusions with heterogeneous appearance and crystalline material. The biliary system is composed of numerous bile ductules and ducts. In the adult lamprey, the biliary system has disappeared. The hepatocytes loose their tubular arrangement and the characteristic differentiation of their biliary pole. In contrast to previous reports in the literature the presence of bile pigment in the adult lamprey liver could not be demonstrated with any histochemical technique.
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    URL: http://dx.doi.org/10.1007/BF00307681
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  • 11
    Electronic Resource
    Electronic Resource
    Histochemical studies on the morphology of the golgi apparatus and its relationship to catecholamine biosynthesis in the locus coeruleus of the rat (1973)
    Springer
    Cell & tissue research 136 (1973), S. 329-348 
    Details
    ISSN: 1432-0878
    Keywords: Locus coeruleus (Rat) ; Neurons ; Golgi apparatus ; Catecholamine biosynthesis ; Histochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Detailed histochemical studies have been performed on the morphology of the Golgi apparatus (GA) by application of the thiamine pyrophosphatase (TPPase) method (Novikoff and Goldfischer, 1961) to the neurons of the locus coeruleus (LC) of normal and catecholamine biosynthesis inhibitors (fusaric acid and D, L-α-methyl-p-tyrosine methylester HCl) given adult healthy male Wistar strain rats. The neurons were classified into five categories on the basis of the morphology of the Golgi apparatus. The number of cells in individual categories was counted to evaluate the percentage of each category in the whole nucleus. The majority of cells belongs to Types II, III, and IV whose GA goes through cyclic activity, but the remaining neurons belong to Types I and V which may have a strong tendency to be different from the former in character. The latter neurons correspond formally with Types I and V of the rabbit LC, but they do not respond to the drugs administered. The rat LC is very similar to the dorsal vagal nucleus of the rabbit in regard to the dominant category. The present results indicate that the majority of the rat LC neurons may work vigorously and they may be motor neurons. Administration of the drugs caused reduction of TPPase activity, augmentation of disintegration and the budding-off process of the GA of Type IV, a decrease in the percentage of Type IV and an increase in that of Type II. Administration of 100 mg/kg fusaric acid caused maximal morphological change of the GA at the 90th minute; however, administration of 200 mg/kg fusaric acid showed more marked change of the GA, having two peaks and two valleys. The GA revealed much more intense reaction to D,L-α-methyl-p-tyrosine methylester HCl than to fusaric acid. The present results indicate that tyrosine hydroxylase may be the rate-limiting enzyme in the catecholamine biosynthesis. These noticeable changes of GA caused by administration of the drugs were completely restricted to the neurons of LC and the neurons of the mesencephalic nucleus of the trigeminal nerve did not show any morphological changes of the GA. These results strongly suggest that the GA of the rat LC neurons may have ability to synthesize catecholamine whereas the GA of the rat mesencephalic nucleus of the trigeminal nerve may be completely devoid of this ability and that the role of the GA may be different depending on the anatomical regions.
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    URL: http://dx.doi.org/10.1007/BF00307038
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  • 12
    Electronic Resource
    Electronic Resource
    Electron microscopic observations on the mucous secretory activity of the human gallbladder epithelium (1973)
    Springer
    Cell & tissue research 139 (1973), S. 463-471 
    Details
    ISSN: 1432-0878
    Keywords: Gallbladder (Human) ; Mucus secretion ; Reverse pinocytosis ; Histochemistry ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Mucous secretory activity of the human gallbladder epithelium was investigated by light and electron microscopy and with histochemical techniques. There are two types of granules in the supranuclear region of the epithelial cells. The one low in density contains a fine filamentous material and gives a strongly positive silver methenamine reaction. The other is dense and only faintly positive. The granules of the former are considered to be mucous secretory granules and the granules of the latter may be lysosomes. PAS positive granules correspond presumably to both types of granules mentioned above. The mucous secretory granules are considered to be synthesized by the Golgi apparatus and the granular endoplasmic reticulum as has been confirmed in other mucous secretory cells. Their content is released from the cell by reverse pinocytosis. Typical goblet cells occur frequently in the fetal epithelium, but cannot be observed in the adult specimens.
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    URL: http://dx.doi.org/10.1007/BF02028388
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  • 13
    Electronic Resource
    Electronic Resource
    Distribution of succinate-, α-glycerophosphate-, NADH-, and NADPH dehydrogenases (tetrazolium reductases) in the hippocampal region of the rat during postnatal development (1973)
    Springer
    Cell & tissue research 141 (1973), S. 347-373 
    Details
    ISSN: 1432-0878
    Keywords: Hippocampal region (rat) ; Distribution of dehydrogenases ; Postnatal development ; Histochemistry ; Light microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Succinate-, α-glycerophosphate-, NADH-, and NADPH dehydrogenases (tetrazolium reductases) were studied histochemically in brain sections. The distribution of these enzymes in the hippocampal region was mapped in different postnatal stages. In addition to the hippocampus and the dentate area which have also been dealt with by other authors, the description includes the entorhinal area, parasubiculum, presubiculum, and subiculum. In all the areas of the hippocampal region the enzyme activity is first localized mainly in cell bodies. Activity in the neuropil shows a tendency to increase with age throughout the stages studied. Examples of particularly early or late differentiation of the histochemical pattern exist. Thus already after 4–6 days the differentiated staining pattern in the hippocampal layers as seen in the adult, begins to appear. In the entorhinal area the early enzyme distribution agrees with that in the adult and facilitates identification of its layers. After 22 days the fundamental enzyme chemoarchitectonics of the adult rat brain has been obtained. Histochemical characteristics of the different areas, support the view that the hippocampal region constitutes an entity.
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    Distribution of acetylcholinesterase in the hippocampal region of the rat during postnatal development (1973)
    Springer
    Cell & tissue research 141 (1973), S. 375-400 
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    ISSN: 1432-0878
    Keywords: Hippocampal region (rat) ; AChE distribution ; Postnatal development ; Histochemistry ; Light microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Acetylcholinesterase (AChE) in the hippocampal region was studied histochemically in different postnatal stages. Enzyme changes are described for the hippocampus proper, the dentate area, the entorhinal area, parasubiculum, area retrosplenialis e, presubiculum, and subiculum. In 1 day old animals there are only traces of AChE in solitary perikarya of the hippocampal stratum radiatum, while there is no activity in the neuropil. This is in contrast to other regions of the brain, e.g. the corpus striatum and the medial septum. Enzyme activity in the neuropil, although very weak, is seen clearly on the 2nd day. While the enzyme reaction in some cell soma seems to be permanent and also present in adult rats, other cell bodies, e.g. in the entorhinal area lose AChE activity during the last part of the 2nd week and in the 3rd week. Similar observations have been made on other parts of the central nervous system. The main features of chemodifferentiation in the hippocampal region seem to be completed by the end of the third week, although most layers and areas have not yet achieved the same high staining intensity as seen in the adult rats.
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  • 15
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    Effects of parathyroid hormone on osteoclasts in vivo (1973)
    Springer
    Cell & tissue research 141 (1973), S. 529-544 
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    ISSN: 1432-0878
    Keywords: Osteoclast ; Parathyroid hormone ; Lysosomes ; Bone resorption ; Electron microscopy ; Histochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Young rats were treated with high doses of parathyroid hormone (PTH). Osteoclasts from these animals revealed characteristic alterations in comparison to control cells: a) The cytoplasm contained large vacuoles with phagocytosed cells, some of which resembled osteoblasts or osteocytes. The vacuoles were interpreted as lysosomes because the engulfed cells often appeared partly digested and the vacuoles contained acid phosphatase as demonstrated histochemically, b) lipid droplets were present in the cytoplasm and usually located close to the endoplasmic reticulum and/or in regions with many free ribosomes, c) the Golgi complex was more frequently separated from the nuclei than in control cells, d) small coated cytoplasmic bodies were numerous in the peripheral cytoplasm, e) the membranes of the endoplasmic reticulum were fused in some places, f) cytoplasmic regions with numerous free ribosomes were frequent, g) large ring-shaped granules occurred in some mitochondria. Energy dispersive X-ray analysis of these granules provided evidence that they contained calcium and probably phosphorus, h) in some osteoclasts the mitochondria were enlarged. — The findings are consistent with an increased activity of osteoclasts and in particular a stimulation of the lysosomal system in these cells.
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  • 16
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    Histochemical demonstration of Δ5-3 ß-OHD activity in the granulosa cells of ovarian follicles of immature and mature mice correlated with some ultrastructural observations (1973)
    Springer
    Cell & tissue research 136 (1973), S. 215-228 
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    ISSN: 1432-0878
    Keywords: Ovary (mouse) ; Granulosa cells ; Steroidogenesis ; Electron microscopy ; Histochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A systematic study of Δ5-3β-hydroxysteroid dehydrogenase in the granulosa cells of immature and mature mice was made. The histochemical results were compared with the ultrastructural findings on the same cells in an attempt to determine whether the granulosa cells are capable of a steroidogenic role. In newborn and immature mice the granulosa cells of a great amount of follicles demonstrated a moderate or strong histochemical activity. In mature mice the granulosa cells demonstrated a weak or moderate activity normally only in preovulatory follicles and in some other atretic follicles. The granulosa cells of the normal developing follicles did not show such activity. In addition the histological control of numerous parallel sections demonstrated particularly in immature ovaries the presence of a great amount of atretic follicles. In the cytoplasm of the granulosa cells of the follicles in immature ovaries only clusters of lipid droplets with ribosomes were noted; while in the preovulatory follicles of mature animals there started to appear mitochondria with tubular cristae, smooth membranes of the endoplasmic reticulum and irregular lipid droplets. In the obviously atretic follicles several granulosa cells as well as theca interna cells showed numerous lipid droplets and ribosomes together with different degenerating organelles. The granulosa cells of the normal developing follicles showed a well developed Golgi complex, granular endoplasmic reticulum and free ribosomes. The histochemical and ultrastructural findings suggest a steroidogenic role of the granulosa cells only in the larger preovulatory follicles (probably related to an early luteinization of this layer) but this role was not demonstrated in the same cells in normal developing follicles. In addition, since an histochemical positivity was demonstrated also in the granulosa cells of some obviously atretic follicles, it is possible that many of the follicles having granulosa cells filled with lipid droplets and attached ribosomes and histochemically positive might be, in the immature ovaries, in a very precocious stage of atresia. It is to precise for these cells whether a cytoplasm with these two strictly correlated components (lipid droplets and attached ribosomes) and showing an histochemical positivity could carry-on all the biochemical steps involved in steroid biosynthesis or only has only a temporary capability to produce some precursors of steroids.
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  • 17
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    The effects of desiccation and starvation upon the weight, histology and ultrastructure of the reproductive tract of Biomphalaria glabrata, intermediate host of Schistosoma mansoni (1973)
    Springer
    Cell & tissue research 136 (1973), S. 229-262 
    Details
    ISSN: 1432-0878
    Keywords: Reproductive tract ; Biomphalaria glabrata ; Desiccation ; Starvation metabolism ; Weighing experiments ; Histochemistry ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The effects of desiccation (36 weeks), starvation (12 weeks) and recovery (4 weeks following 16 weeks of desiccation and 2 weeks following 9 weeks of starvation) upon the accessory sex glands and some other body parts of Biomphalaria glabrata were studied by means of weighing experiments and histochemical and ultrastructural methods. The results of the weighing experiments show that the various parts of the reproductive tract are not protected from involution processes during the adverse conditions, as some parts (the albumen gland, spermduct and prostate gland) showed a more pronounced decrease in weight than other body parts. Measurements of the epithelial heights of the accessory sex glands and ultrastructural observations corroborate the results of the weighing experiments. During the adverse conditions the organelles involved in the formation of the secretion granules (granular endoplasmic reticulum and Golgi-apparatus) became involuted. The secretion granules within the cells of the albumen gland, spermduct and prostate gland were broken down by crinophagy. This latter process was less pronounced in the oviduct, muciparous gland, oöthecal gland, uterus and vagina. During the adverse conditions the lipoprotein producing cell types (mainly in the male part) remained active longer than the polysaccharide producing cell types (mainly in the female part). The significance of this observation is discussed. The amounts of glycogen present in most tissues of the reproductive tract and of the other investigated body parts (the foot, columellar muscle and vesicular connective tissue cells) were not apparently diminished, even when the secretory cells showed clear signs of involution, as a result of long periods of adverse conditions. Within the recovery periods 50% of the snails resumed egg production. During recovery the relative increases in weight of the accessory sex glands seemed to exceed that of other body parts, indicating that the restoration of the accessory sex glands is favoured. Histologically, reactivation of the accessory sex glands was apparent after 36–72 hours of recovery. At this time secretory material was again observed within the cisternae of the gland cell Golgi-bodies. Because reactivation is fairly rapid, a nervous or hormonal control of this process appears more likely than control via the general metabolism. The possible functions of various body organs in storing food reserve material is discussed, and it is concluded that the glycogen storing vesicular connective tissue cells (which occur throughout the whole body in the connective tissue) play a major role.
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  • 18
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    Development and differentiation of fetal rat sensory ganglia and spinal cord segments in vitro (1973)
    Springer
    Cell & tissue research 144 (1973), S. 339-351 
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    ISSN: 1432-0878
    Keywords: Nervous system ; Tissue culture ; Sheath cells ; Sensory end-organs ; Histochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Fetal spinal ganglia and spinal cord segments with adhering spinal ganglia were explanted on collagen-coated coverslips. They were investigated with enzyme histochemical methods for the existence of hydrolases and dehydrogenases up to 54 days of cultivation. Alkaline phosphatase was located in arachnoid cells and in mantle cells (satellite cells). Butyrylcholinesterase and alpha-glycerophosphate-menadione-tetrazolium reductase were found in mantle cells. Acetylcholinesterase and indoxylesterase were active in the whole neuron; acid phosphatase and alpha-naphthylacetate esterase were restricted to the perikarya. During the process of cell differentiation in vitro alkaline phosphatase decreased in mantle cells. Acid phosphatase became diminished distinctly in spinal ganglion cells. The other neuronal enzymatic activities remained unaltered during the whole period of cultivation. Proliferated Schwann cells were conspicuous by their activity for butyrylcholinesterase. In newly formed myelin sheaths arylsulphatase was active. Lactate dehydrogenase was contained in the perineurium which had developed. Cultures of long duration could contain cytological formations which were reminiscent of sensory end-organs with respect to their enzyme patterns. The enzyme activities of nervous tissues in vitro in their approximation to the situation in situ are discussed.
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  • 19
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    On the histology and histochemistry of the snout and vibrissae of the common shrew (Sorex araneus L.) (1972)
    Springer
    Cell & tissue research 124 (1972), S. 445-453 
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    ISSN: 1432-0878
    Keywords: Tactile hair system ; Snout ; Shrew ; Histology ; Histochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The structure of the snout and tactile hair system of the common shrew (Sorex araneus) and the distribution of cholinesterase and alkaline phosphatase activity were studied. The structure of the vibrissae is exceptional compared with that of other mammals studied. Differences are the attachment system of hair follicle to the capsule, the impossibility of dividing the blood sinus clearly into cavernous sinus and ring sinus, the lack of continuous ring-wulst, clear constriction in the outer root sheath at the neck of hair follicle and some specialized structures connected with this. Alkaline phosphatase was not found in the nervous structures of the vibrissae but cholinesterase activity occurs at least in two different structures upon or in the outer root sheath of the hair follicle. The role of these structures and the significance of vibrissae to the shrew is discussed.
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    URL: http://dx.doi.org/10.1007/BF00335250
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  • 20
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    Long-term cell culture of two differentiated cell types from the liver of larval and adult Xenopus laevis (1972)
    Springer
    Cell & tissue research 128 (1972), S. 457-469 
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    ISSN: 1432-0878
    Keywords: Liver ; Amphibian ; Tissue culture ; Histochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Two distinct types of cells were derived from organ cultures of liver from adult and larval Xenopus laevis. Each type was isolated in clonal cell culture. Several media were compared with respect to support of epithelioid outgrowths from explants and support of growth of epithelioid colonies in cell culture. Ultracentrifuged embryo extract promotes the growth of all cell types, but the particulate fraction is also required for the maintenance of the epithelioid morphology of larval cells. In these media it was possible to maintain some epithelioid cell cultures for over 6 months. The identity and retention of some specialized functions of both cell types were demonstrated on larval cells. One cell type contained PAS-stainable, amylase-sensitive granules that increased in amount after treatment with glucocorticoids. This same type was shown by histochemical methods to contain phosphorylase, glucose-6-phosphatase, and dexamethasone-inducible tyrosine aminotransferase, and is considered to be a hepatocyte. The second type appears to be a sinusoidal cell, since it phagocytosed trypan blue and stained positively for acid phosphatase.
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  • 21
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    Histochemistry and Ultrastructure of the Epidermis and the Subepidermal Gland Cells of the Freshwater Snails Lymnaea stagnalis and Biomphalaria pfeifferi (1972)
    Springer
    Cell & tissue research 130 (1972), S. 93-134 
    Details
    ISSN: 1432-0878
    Keywords: Epidermis ; Snails (Lymnaea stagnalis, Biomphalaria pfeifferi) ; Histochemistry ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The epidermis and the associated subepidermal gland cells of the freshwater snails Lymnaea stagnalis and Biomphalaria pfeifferi were studied by means of histochemical and electron microscope techniques. The single cell layered epidermis is composed of general epidermal cells, cilia cells and a few scattered goblet cells. The foot sole and the epidermal regions of the pneumostome and the ventral surface of the lips near the mouth consist nearly entirely of cilia cells; elsewhere the cilia cells are found scattered among the general epidermal cells. The apical layer of the general epidermal cells bear microvilli. Numerous mitochondria, vesicles and lysosomes are located in the apical region of the cells. Several Golgi bodies and a poorly developed granular endoplasmic reticulum occur in the supranuclear region; the nucleus lies in the basal part of the cell. The general epidermal cells in the mouth region contain numerous microfilaments compared to the general epidermal cells in the rest of the epidermis. The cilia of the cilia cells in the densely ciliated regions possess well developed roots and basal bodies interconnected by means of the basal feet. With regard to the other cell organelles, cilia cells are quite similar to the general epidermal cells. For comparison a brief study of the ultrastructure of the epidermis of the terrestrial snail Helix aspersa was carried out. The skin of the snail is covered by a mucous layer produced by various gland cells. In L. stagnalis, in addition to the epidermal goblet cells, thirteen subepidermal gland cell types could be distinguished. The histochemistry of the gland cell types is reflected in the ultrastructure. Three of the gland cell types have an ubiquitous distribution, four types are peculiar to the foot, two types to the lips and five types to the mantle. In B. pfeifferi one epidermal gland cell type and only seven subepidermal gland cell types could be distinguished. Most of these gland cells are limited in their distribution to the foot, lips and mantle edge. The observations may provide a basis for further study in the functions of the snail epidermis.
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    Les glandes exocrines des termites (1972)
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    Cell & tissue research 130 (1972), S. 205-218 
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    ISSN: 1432-0878
    Keywords: Sternal gland ; Trinervitermes geminatus ; Cell types ; Histochemistry ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Résumé La glande sternale de Trinervitermes geminatus correspond à un épaississement médian de la partie antérieure du cinquième sternite abdominal. Elle est bordée intérieurement par une fine basale conjonctive et extérieurement par la cuticule, quelques sensilles campaniformes sont également visibles dans la masse glandulaire. On peut y reconnaître deux types de cellules: d'abord des cellules ovalaires situées dans la partie moyenne de la glande. Elles contiennent de grosses mitochondries et des globules denses, et sont en relation avec la basale par quelques fins prolongements cellulaires. Ensuite des cellules cylindriques, bien développées, constituant toute l'épaisseur de la glande. Elles possèdent de nombreuses vésicules de reticulum lisse et une bordure en brosse formée de microvillosités et de digitations cytoplasmiques qui pénètrent dans la cuticule. Cette cuticule comprend une mésocuticule lacunaire et une fine épicuticule percée par des «canalicules épicuticulaires». Une comparaison est établie avec la glande sternale d'autres termites, en particulier celle de Kalotermes.
    Notes: Summary The sternal gland of Trinervitermes geminatus appears as a median thickening of the epidermis at the anterior part of the fifth abdominal sternite. It is lined internally with a thin basal lamina and externally with the cuticle. A few campaniform sensilla are also visible in the glandular mass. Two types of cells may be recognized. First, oval-shaped cells, containing numerous large mitochondria and dense globules; these cells having no contact with the cuticle are connected with the basal lamina by a few narrow cytoplasmic stems. Second, tall columnar cells, with vesicular endoplasmic reticulum and an apical brush border built up by microvilli and finger—like processes which reach into the cuticle. This cuticle is made of alveolous mesocuticle and a thin epicuticle perforated by “epicuticular canals”. The organ is compared with the sternal gland of other termites, particularly with the one of Kalotermes.
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    On the harderian gland of the domestic duck (Anas platyrhynchus) (1972)
    Springer
    Cell & tissue research 133 (1972), S. 223-230 
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    ISSN: 1432-0878
    Keywords: Harderian gland ; Anas platyrhynchus ; Polysaccharides ; Histology ; Histochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The parenchyma of the Harderian gland of the domestic duck consists of numerous tubular terminal portions, lined by a simple columnar epithelium. Its secretory surface is increased by intratubular folds. Within the cytoplasm of the epithelial cells secretory granules are observed. Polysaccharides of different nature are demonstrated. Strikingly, all centrally located cells contain a periodate reactive mucin. The successive administration of the PAS reaction and of Alcian Blue reveals the coexistence of acid and neutral mucins in the same cells. A metachromatic reaction of the mucosubstances at pH 1.0 was observed and the presence of acid sulfated groups in the Harderian gland, as demonstrated byAlcian Blue at pH 0.5, thereby confirmed. There was no glycogen reaction.
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    Über den Peroxydasetransport im Darmepithel der Ratte (1972)
    Springer
    Cell & tissue research 134 (1972), S. 403-410 
    Details
    ISSN: 1432-0878
    Keywords: Small intestine (rat) ; Transport of peroxidase ; Epithelium ; Histochemistry ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Bei Ratten tritt 3 min nach intravenöser Injektion von Peroxydase elektronenmikroskopisch ein entsprechendes Reaktionsprodukt im Kapillarlumen der Lamina propria des Dünndarms und an der Basalmembrangrenze der Saumepithelzellen auf. 5 min nach der Injektion finden sich im basalen Abschnitt des Darmepithels pinozytotische Bläschen mit dem Peroxydase-Reaktionsprodukt. — 10–30 min nach der Injektion erreichen die Partikel die apikalen Teile der Zelle. Sie dringen in den interzellulären Spalten bis zu den Haftplatten vor, erreichen jedoch nie das Darmlumen. Im Dünndarm existiert vermutlich auch ein der Resorption entgegengesetzter Saftstrom, der durch Peroxydase markiert werden kann.
    Notes: Summary In rats, 3 minutes after intravenous injection of peroxidase the reaction product can be observed electronmicroscopically in the lumina of the capillaries of the small intestine as well as at the border of the basement membrane of the epithelial border cells. Pinocytotic vesicles containing peroxidase particles occur in the basal portion of the epithelium of the small intestine 5 minutes after injection. 10–30 minutes later, the peroxidase reaches the apical region of the cell. The particles infiltrate into the intercellular spaces as far as the tight junctions but never reach the intestinal lumen. In the small intestine there probably exists a flow of fluid in opposite direction to the resorption, which can be marked by peroxidase.
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    Topochemischer Nachweis von carbonylhaltigen Verbindungen im Thymus (1973)
    Springer
    Cell & tissue research 142 (1973), S. 465-477 
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    ISSN: 1432-0878
    Keywords: Rabbit thymus ; Ketosteroids ; Granulated cells ; Histochemistry ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Topochemisch konnten im Kaninchenthymus Ketosteroide nachgewiesen werden, für deren Vorhandensein die chemische Analyse von Thymuslipidextrakten Hinweise ergeben hatte. Die Darstellung der Ketosteroide erfolgte mit der NAHD-Reaktion (Camber, 1949). Diese Befunde dürften als spezifisch gelten, da eine Verfälschung durch freie Gewebsaldehyde, Plasmalogene (Gomori, 1952) und Corticosteroide (Khanolkar et al., 1958) ausgeschlossen wurde. Mit Hilfe der Camber-Methode konnten Granula bestimmter Zellen des Kaninchenthymus selektiv dargestellt werden, die auf Grund morphologischer Kriterien in zwei Gruppen unterteilt wurden. Die Zellen liegen im gesamten Thymusparenchym sowie im Bindegewebe der Septen und der sogenannten „Kapsel“. Prädilektionsorte stellen die Umgebung der Hassallschen Körperchen, die Mark-Rinden-Grenze, der intra- und perivasale Raum sowie die subkapsuläre Zone dar. Beide Zellgruppen sind Sudan III-, PAS- und Eosin-positiv, besitzen eine ausgeprägte gelbgrüne Eigenfluoreszenz und können differentialdiagnostisch gegen Mastzellen abgegrenzt werden. Elektronenmikroskopisch zeigen beide Zellgruppen zahlreiche Übereinstimmungen mit Reifestadien eosinophiler Knochenmarkszellen.
    Notes: Summary Ketosteroids were demonstrated topochemically in the rabbit thymus following indications given by chemical analysis of thymus lipid extracts. They were revealed by means of the NAHD-reaction (Camber, 1949). The results are thought to be specific, because adulteration by free tissue aldehydes, plasmalogens (Gomori, 1952) and corticosteroids (Khanolkar et al., 1958) can be excluded. The Camber-method selectively reveals the presence of two groups of morphologically differentiable granules in specific rabbit thymus cells which are distributed throughout the entire thymic parenchyme as well as in the connective tissue of the septa and the in so-called “capsule”. They are mainly seen in the vicinity of Hassall's bodies, in the zone between medulla and cortex, the intra- and perivascular space and in the subcapsular space. Both cell groups are Sudan III-, PAS- and eosin-positive, show intensive yellow-green primary fluorescence and can be distinguished from mast cells by differential diagnosis. Electron microscopy reveals that both cell groups show many similarities with the maturation phase of bone marrow eosinophils.
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    Fine structure and innervation of the avian adrenal gland (1973)
    Springer
    Cell & tissue research 145 (1973), S. 389-416 
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    ISSN: 1432-0878
    Keywords: Adrenal chromaffin cells ; Ganglion cells ; Avian adrenal gland ; Light- and electron microscopy ; Histochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The fine structure of the adrenal gland of 15 bird species was investigated by light-, fluorescence-, and electron microscopy as well as by histochemical methods. 1. Adrenal Chromaffin Cells. Cells producing adrenaline (A) and noradrenaline (NA) may be distinguished from each other with the electron microscope by the different structure of their granules. In all species investigated A-cells prevailed. Granules in A-cells of Corvidae, partly those of the other passeriform birds as well, resemble granules from the pituitary pars intermedia because of their incomplete membrane and the low electron density of their contents. A-cells of some passeriform birds have mitochondria with specifically arranged cristae. Large cytosomes and inclusions of lipofuscin pigment are to be found in the gulls Larus ridibundus and Larus argentatus. The House Sparrow (Passer domesticus) and the Chaffinch (Fringilla coelebs) show vacuoles filled with a colloid-like substance, which are surrounded by adrenal chromaffin cells. 2. Ganglion Cells. The adrenal gland of birds is rich in ganglion cells. Two types can be distinguished. “Large” ganglion cells resemble completely sympathetic nerve cells as described in many vertebrates. “Small” ganglion cells have a low nucleus-cytoplasm-ratio. Some of them include granules of various structure and quantity. Whether they represent transitional stages to adrenal cells, interneurons, P-neurons or a heterogeneous population remains to be clarified.
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    Fine structure and innervation of the avian adrenal gland (1973)
    Springer
    Cell & tissue research 145 (1973), S. 417-442 
    Details
    ISSN: 1432-0878
    Keywords: Adrenal chromaffin cells ; Birds ; Cholinergic innervation ; Histochemistry ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary According to their ultrastructure and histochemistry three types of efferent nerve fibers can be distinguished in the bird's adrenal gland. The main part is made up of cholinergic fibers recognizable by a positive reaction for acetylcholinesterase and two specific populations of granules within the synaptic ending. Synaptic vesicles measuring 300 to 500 Å in diameter and dense-cored vesicles with a diameter of about 1 000 Å are discernible. In the periphery of the gland cholinergic axons for the innervation of adrenal cells form large bundles surrounded by a perineural sheath. The bundles cross the capsule and are situated within the adrenal chromaffin cords or at their periphery. Finally small groups of fibers enter a group of chromaffin cells which are surrounded by a basal lamina and which consist of about a dozen or more cells producing adrenaline and noradrenaline. Synaptic endings occur, above all in passeriform species, in the center of a chromaffin cell complex. They are either attached to the innervated cells or their dendrite-like processes, or embedded into the cells, or connected to short spines of the innervated cells. Synaptic and dense-cored vesicles leave the bouton by exocytosis. One synaptic terminal may innervate up to three A- or NA-cells. The existence of different types of synapses for A- and NA-cells cannot be excluded.
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    Axoplasmic flow of adrenaline and monoamine oxidase in amphibian sympathetic nerves (1972)
    Springer
    Cell & tissue research 124 (1972), S. 44-56 
    Details
    ISSN: 1432-0878
    Keywords: Axonal transport ; Toad spinal nerves ; Ligation ; Sympathectomy ; Histochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The accumulation of both A and MAO proximal to a ligature on toad spinal nerves has been shown to occur at a slower rate than in mammals. As in mammals, there are two components of axonal transport in amphibian nerves, with the accumulation of A reaching a peak at between 4 and 7 days (cf. 2–4 days for NA in mammals), while MAO accumulation does not reach its maximum before 9 days (cf. 7 days in mammals). No accumulation occurs after sympathectomy, providing evidence for localization of MAO within amphibian sympathetic adrenergic nerves. Distal accumulation of MAO occurs in toad sympathetic nerves; this has not been reported to occur in mammalian nerves. Distal accumulation reaches a peak at 2–4 days, which suggests either a fast retrograde flow of MAO or that induction of MAO is occurring. These results are discussed in relation to differences between mammalian and amphibian sympathetic nerves and to the events occurring following ligation of these nerves.
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    URL: http://dx.doi.org/10.1007/BF00335453
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    Studies on the cytophysiology of the fat body of the American silkmoth (1972)
    Springer
    Cell & tissue research 124 (1972), S. 433-444 
    Details
    ISSN: 1432-0878
    Keywords: Insect ; Fat body ; Development ; Protein body ; Electron microscopy ; Histochemistry ; Lipoprotein ; Cytology ; Lipid globule ; Silkworm
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A developmental study at the electron microscopic level was conducted of the fat body cells of Hyalophora cecropia (L.). During the last larval instar the fat body increases in volume and the cells exhibit a well developed rough endoplasmic reticulum and protein bodies of diverse sizes. In the pupal fat body, the protein bodies appear to be enclosed by a double membrane and contain glycogen granules, ribosomes and mitochondrion-like structures. In addition, there are large lipid globules, cytolysomes and rough endoplasmic reticulum. The ultrastructure of the protein bodies suggests the development of large bodies by fusion of smaller protein bodies. Changes in fat body cell ultrastructure were followed during adult development and cytological evidence was obtained for the depletion of protein, glycogen and lipid in the female during this period. The female adult fat body cell contains free ribosomes, protein bodies, many mitochondria, a few lipid globules and glycogen granules. The male moth fat body cells have many mitochondria, a few glycogen granules, essentially no protein bodies, but an abundance of large lipid globules. Studies on the influence of egg maturation on the morphology of the fat body of Hyalophora gloveri (L.) revealed that ovariectomy of pupae yielded adults having more fat body than normal females, and that the fat body cells of the ovariectomized animals contained more glycogen, lipid and protein. Male pupae receiving ovarian implants developed into adults containing eggs and possessed more fat body than normal females but less than normal males. Very few glycogen granules were found in the fat body cells of normal males or males with implanted ovaries.
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    URL: http://dx.doi.org/10.1007/BF00335249
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    I. Ultrastructure of acinar cell granules in mammalian submaxillary glands (1972)
    Springer
    Cell & tissue research 126 (1972), S. 320-334 
    Details
    ISSN: 1432-0878
    Keywords: Glandulae submaxillares ; Mammals ; Acinar cell granules ; Ultrastructure ; Histochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Granules in acinar cells show considerable variations in size, shape, electron-density and molecular organisation of secretory material. Discrete organelles with an electron-dense homogenous matrix are seen in the guinea-pig and to some extent in the male hamster. Similar organelles with moderate electron-density are seen in the cat and dog. Acinar cells of the cat, hamster, guinea-pig and to a lesser extent the rat, contain discrete, pale granules and also confluent organelles arising from two to three of the pale particles. Composite, electron-pale secretory units are observed in the dog and rabbit. No correlation could be elicited between the histochemical reactivity of the acinar cells and the content of the secretory enzymes. The relationship between the histochemical reactivity and ultrastructural appearance of acinar cell granules and the organelles containing kallikrein, trypsin-like proteases and amylase seems much more important and functionally relevant. Morphological aspects of the intracellular transport and secretion of granules are discussed.
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    URL: http://dx.doi.org/10.1007/BF00306847
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    Axoplasmic flow of adrenaline and monoamine oxidase in amphibian sympathetic nerves (1972)
    Springer
    Cell & tissue research 124 (1972), S. 44-56 
    Details
    ISSN: 1432-0878
    Keywords: Axonal transport ; Toad spinal nerves ; Ligation ; Sympathectomy ; Histochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The accumulation of both A and MAO proximal to a ligature on toad spinal nerves has been shown to occur at a slower rate than in mammals. As in mammals, there are two components of axonal transport in amphibian nerves, with the accumulation of A reaching a peak at between 4 and 7 days (cf. 2–4 days for NA in mammals), while MAO accumulation does not reach its maximum before 9 days (cf. 7 days in mammals). No accumulation occurs after sympathectomy, providing evidence for localization of MAO within amphibian sympathetic adrenergic nerves. Distal accumulation of MAO occurs in toad sympathetic nerves; this has not been reported to occur in mammalian nerves. Distal accumulation reaches a peak at 2–4 days, which suggests either a fast retrograde flow of MAO or that induction of MAO is occurring. These results are discussed in relation to differences between mammalian and amphibian sympathetic nerves and to the events occurring following ligation of these nerves.
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    URL: http://dx.doi.org/10.1007/BF00981940
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    La structure fine des caecums rectaux de deux Asteriidae: Marthasterias glacialis (L) et Coscinasterias tenuispina (Lam) (Echinodermata, Asteroidea) (1972)
    Springer
    Cell & tissue research 128 (1972), S. 366-384 
    Details
    ISSN: 1432-0878
    Keywords: Rectal caeca ; Starfishes ; Epithelium ; Histochemistry ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Résumé Dans les caecums rectaux des deux espèces étudiées, stratification tissulaire et composition cellulaire sont semblables. L'épithélium interne se compose de mucocytes et de cellules banales. Outre du glycogène, ces dernières renferment chez M. glacialis des grains de mucopolysaccharides neutres associés à des protéines tandis que chez C. tenuispina, les granules contiennent des mucopolysaccharides acides carboxylés et sulfatés également associés à des protéines. L'épithélium externe de C. tenuispina présente, outre des cellules banales, des cellules glandulaires à contenu protéique. Une attention particulière a été apportée à l'ultrastructure du pôle apical des cellules banales internes. On y remarque de nombreuses microvillosités et un flagelle central caractéristique. De plus, on observe très fréquemment des formations pinocytaires ainsi que plus rarement de la phagocytose. Les diverticules seraient donc, chez les Asteriidae, des organes digestifs à part entière se caractérisant par un grand pouvoir d'absorption et une digestion intracellulaire. Enfin, le pôle basal des cellules banales internes, très contourné, contient, par endroit, des β-cytomembranes typiques. Il est possible qu'à ce niveau aient lieu des transferts de substances vers le coelome.
    Notes: Summary Histological and cytological composition are similar in the rectal caeca of both studied species. Internal epithelium is composed of mucocytes and of banal cells. Besides glycogen, these cells contain in M. glacialis neutral mucopolysaccharides associated with proteins, while in C. tenuispina, the granules contain acidic carboxyled and sulfated mucopolysaccharides also associated with proteins. The external epithelium of C. tenuispina presents, besides banal cells, granular secretory cells with proteic content. Special attention has been payed to the ultrastructure of the apical pole of the internal banal cells. One notices numerous microvilli and a characteristic central flagellum. Moreover, one frequently observes pinocytic formations as well as—more rarely—phagocytosis. The rectal diverticula would thus be, in the Asteriidae, real digestive organs provided with a great capacity of absorption and intracellular digestion. The basal pole of the internal banal cells contains from place to place typical β-cytomembranes. Transfers of substances to the coelom can occur at that level.
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    URL: http://dx.doi.org/10.1007/BF00306976
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    Experimentelle studien über den Myofer®-Transport durch das Pansenepithel der Ziege (1972)
    Springer
    Cell & tissue research 129 (1972), S. 561-569 
    Details
    ISSN: 1432-0878
    Keywords: Rumen epithelium (Goat) ; Permeability ; Transport of Myofer ; Histochemistry ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Durch submuköse Injektion von Myofer, einer licht- und elektronenmikroskopisch nachweisbaren Testsubstanz, wurde in vivo die Permeabilität des Pansenepithels untersucht. Folgende Befunde wurden erhoben: 1. Kurz nach der Applikation ist Myofer in allen Schichten des Epithels nachweisbar. Der Transport dieser Substanz ins Stratum corneum wird durch die tiefen Hornzellen der Barriere nicht verhindert. 2. In allen Epithelschichten ist das Myofer in Form intrazellulärer Cytosomen anzutreffen. 3. In den Interzellularräumen sind Myofergranula zwischen Basalmembran und den tiefen Hornzellen nur vereinzelt, zahlreicher jedoch jenseits der Barriere zu beobachten. Es wird gefolgert, daß die Testsubstanz Myofer die epitheliale Barriere durch transzelluläre Passage überwindet.
    Notes: Summary The permeability of the rumen epithelium to Myofer, a marker injected submucously in vivo, was studied in the light and electron microscopes. The following observations were made: 1. Myofer penetrates the ruminal epithelium 10 min after injection and can be found in all epithelial layers. The deep horny cells of the barrier layer do not prevent the penetration by this marker of the stratum corneum. 2. Myofer is present as cytosomes in cells of all epithelial layers. 3. The intercellular spaces between the basal lamina and the deep horny cells contain only few Myofer granules; the granules are more numerous on the other side of the barrier layer. The observations suggest that the marker Myofer passes through the epithelial barrier of the ruminal wall by transcellular passage.
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    URL: http://dx.doi.org/10.1007/BF00316750
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    Adenosine triphosphatase activity in the carotid body of the cat (1972)
    Springer
    Cell & tissue research 130 (1972), S. 455-462 
    Details
    ISSN: 1432-0878
    Keywords: Carotid body ; Cat ; ATPase ; Histochemistry ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Three kinds of nucleoside phosphatases were demonstrated histochemically in the cat carotid body with nucleoside triphosphate, nucleoside disphosphate and nucleoside monophosphate as substrates. Each of these enzyme activities exhibited the substrate specificity respectively. The nucleoside triphosphatase activity showed specific localization in association with the parenchymal cells of the carotid body. The electronmicroscopy revealed that the reaction product was located on and between the two apposing plasma membranes of type I and type II cells, of a type II cell and its wrapping axons and of the intricate basal infolding of a type II cell itself. Some possible functions of the adenosine triphosphatase in the carotid body are discussed.
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    URL: http://dx.doi.org/10.1007/BF00306999
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    Les cellules à calcium du conjonctif de Ferrissia wautieri (Moll. Ancylidae) (1972)
    Springer
    Cell & tissue research 134 (1972), S. 227-243 
    Details
    ISSN: 1432-0878
    Keywords: Calcium cells ; Mollusca (Ferrissia wautieri) ; Histochemistry ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Résumé Le calcium s'accumule dans les tissues de Ferrissia wautieri sous forme de sphérules de calcaire intracellulaires. Notre travail comporte une étude descriptive de ces cellules en microscopie optique et électronique, et une étude minéralogique abordée par des techniques de coloration, de dissolution, de polarisation de la lumière et de diffraction électronique. Les résultats obtenus montrent que les sphérules, par leur aspect, leur nombre, leur volume et leur état de cristallisation, présentent des caractères différents selon la saison, ce qui permet d'établir une relation entre l'état des nodules calcaires et les variations du métabolisme des individus.
    Notes: Summary In Ferrissia wautieri calcium is stored in the connective tissue in intracellular calcareous spherules. The cells have been studied light- and electronmicroscopically and a mineralogical analysis of the spherules was done by selective staining, dissolution, polarisation and electron diffraction. The results of these studies show that the aspect, the number, the volume and the rate of cristallisation of the calcospherites are different according to the seasons. Therefore it may be assumed that the characteristics of the spherules are connected with metabolic variations in these animals.
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    URL: http://dx.doi.org/10.1007/BF00307155
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    Elektronenmikroskopische und histochemische Untersuchungen zur Funktion der Branchialdrüse (Parabranchialdrüse) der Cephalopoda (1973)
    Springer
    Cell & tissue research 139 (1973), S. 253-269 
    Details
    ISSN: 1432-0878
    Keywords: Branchial gland ; Cephalopoda ; Fine structure ; Hemocyanin ; Histochemistry ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Licht- und elektronenmikroskopische Untersuchungen an der Branchialdrüse verschiedener Cephalopoden (Sepia officinalis, Octopus vulgaris, Loligo vulgaris, Eledone moschata) zeigen, daß das Drüsengewebe des stark vaskularisierten Organs aus einem sekretorisch aktiven Zelltypus mit ausgeprägtem endoplasmatischen Retikulum besteht. In den Zisternen des endoplasmatischen Retikulums lassen sich granuläre und ringförmige Strukturen (Durchmesser: 45–65 Å und 170 Å) nachweisen, die in Größe und Gestalt mit den Hämocyanin-Einheiten in den Gefäßen und Lakunen übereinstimmen und durch randlichen Zerfall der Zellen in den Blutraum auszuwandern scheinen. Die histochemischen Untersuchungen ergeben eine positive Reaktion für Monoaminoxydase, aber vergleichsweise geringe Phosphatase- und Dehydrogenase-Aktivitäten. Diese Befunde sowie der histochemisch nachgewiesene hohe Kupfergehalt des Drüsengewebes sprechen für eine hämopoetische, d.h. hämocyaninbildende Funktion der Kiemendrüse.
    Notes: Summary Light and electron microscopic investigations on the branchial gland of different Cephalopoda (Sepia officinalis, Octopus vulgaris, Loligo vulgaris, Eledone moschata) show that the gland tissue, strongly vascularized, consists of an actively secreting cell type with a remarkable endoplasmatic reticulum. In the cisternae of the endoplasmatic reticulum granular and ring-shaped structures can be identified (diameter: 45–65 Å and 170 Å) which correspond in size and shape with the hemocyanin units in the vessels and lacunae and which seem to emigrate into the blood space caused by the peripheral decay of the cells. Histochemical investigations show a positive reaction for monoaminoxidase, but comparatively low phosphatase and dehydrogenase activities. These results, and also the high content of histochemically identified copper in the gland tissue, seem to indicate a hemopoetic, i.e. hemocyanin-forming function for the branchial gland.
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    URL: http://dx.doi.org/10.1007/BF00306525
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    The distribution of monoamines in the tel-, di-and mesencephalon of Xenopus laevis tadpoles, with special reference to the hypothalamo-hypophysial system (1973)
    Springer
    Cell & tissue research 137 (1973), S. 521-540 
    Details
    ISSN: 1432-0878
    Keywords: Brain ; Hypothalamo-hypophysial system ; Xenopus laevis, tadpoles ; Monoamines ; Histochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In tadpoles of Xenopus laevis the histochemical distribution of monoamines in the tel-, di- and mesencephalon was studied. Catecholamines are present in some mitral cells of the olfactory bulb, the neurons of the preoptic recess organ (PRO), the neurons of the paraventricular organ (PVO) and cells in the vicinity of the PVO, namely the so-called PVO-accompanying cells, the nucleus infundibularis dorsalis (NID) and in the nucleus reticularis mesencephali (NRM). The PRO, PVO, and NID are composed of liquor-contacting neurons. With the Falck-technique, in the PVO and NID two types of fluorescent cells were observed: green cells, containing a catecholamine and yellow-orange cells, presumably containing a tryptamine. Tryptaminergic neurons occur in the NRM and in the area of the raphe nucleus. Extensive tracts of fluorescent fibres originating in the hindbrain ascend to the area praeoptica and the septum, striatum and epistriatum of the telencephalon. Also, fibre connections could be traced from the PVO to the area praeoptica, to the thalamus and to the nucleus ventromedialis tuberis. The PVO and/or NID give rise to a catecholaminergic tract running towards the hypophysis. Fibres of this tract partly terminate in the developing median eminence and partly in the pars intermedia. Experimental data indicate that the aminergic fibres in the pars intermedia exert an inhibitory influence on MSH-release.
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    URL: http://dx.doi.org/10.1007/BF00307228
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    Cholinesterase-containing nerve fibres on blood vessels in lungs of some laboratory mammals (1973)
    Springer
    Cell & tissue research 140 (1973), S. 91-100 
    Details
    ISSN: 1432-0878
    Keywords: Pulmonary and bronchial vessels ; Laboratory mammals ; Cholinesterase-containing nerve fibres ; Species differences ; Histochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Distribution of cholinesterase-containing nerve fibres on blood vessels in the lungs of rats, mice, rabbits, guinea pigs, cats, dogs, and rhesus monkeys has been studied with the thiocholine method modified by Coupland and Holmes and with the “direct-coloring” method according to Karnovsky and Roots. The presence of acetylcholinesterase positive nerve fibres on the pulmonary arteries has been established only in four species of the studied animals, namely the cat, the dog, the rhesus monkey and the rabbit. These nerves form d distinct plexus on the border between the media and adventitia. In the thick walled perihilar branches of the pulmonary artery of the rabbit the nerve fibres penetrate—as a rule—into the outer half of the media. Despite many times repeated experiments and careful investigations no nerve fibres have been found on the intrapulmonary branches of the blood vessels in the lungs of rats, mice, and guinea pigs. In the walls of the pulmonary veins, with the exception of the pulmonary veins in the rat and the mouse, cholinesterase fibres have been identified only seldom. Acetylcholinesterase nerve fibres in medio-adventitial localization have also been found in the walls of bronchial arterioles, this being the case in all the species under study. Bronchial veins do not exhibit any nerve fibres. The distribution of the acetylcholinesterase-containing nerve fibres on the pulmonary arteries is different from species to species.
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    URL: http://dx.doi.org/10.1007/BF00307060
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    Aspects histologique, histochimique et ultrastructural des péricaryones neurosécréteurs latéraux du protocérébron de Locusta migratoria migratorioides (Insecte: Orthoptère) (1973)
    Springer
    Cell & tissue research 141 (1973), S. 75-91 
    Details
    ISSN: 1432-0878
    Keywords: Neurosecretory cells ; Lateral ; Locust ; Histochemistry ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Résumé L'étude histologique, histochimique et ultrastructurale des péricaryones neurosécréteurs latéraux de Locusta précise leur nombre et leur localisation. Elle révèle que le neurosécrétat latéral est semblable morphologiquement (forme et diamètre des granules élémentaires) au neurosécrétat A de la pars intercerebralis. Cependant, il en diffère histochimiquement par la présence d'une composante glucidique. Elle confirme l'hypothèse émise pour les péricaryones neurosécréteurs de la pars intercerebralis (Girardie et Girardie, 1967), de l'unicité cellulaire chez Locusta des types A et B selon la nomenclature de Johansson (1958). Elle suggère que les péricaryones latéraux pourraient être des cellules neurosécrétrices très actives.
    Notes: Summary The number (8 to 12) and position of the lateral neurosecretory cells have been established in Locusta by a histological, histochemical and ultrastructural study. The neurosecretory material of the lateral cells contains glycoprotein and is, in this way, histochemically different from medial A cells neurosecretory material. However, the morphological aspect (shape, diameter) of elementary dense core vesicles in the lateral and medial A neurosecretory cells is similar. This study confirms the idea (Girardie and Girardie, 1967) that, in Locusta, A cells and B cells (Johansson, 1958) are in fact two physiological aspects of one cell type. It also suggests that the lateral cells could be very active neurosecretory cells.
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    URL: http://dx.doi.org/10.1007/BF00307397
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    Parasympathetic and sympathetic postganglionic synapses in ureterovesical autonomic pathways (1973)
    Springer
    Cell & tissue research 146 (1973), S. 147-154 
    Details
    ISSN: 1432-0878
    Keywords: Peripheral autonomic innervation ; Ureterovesical ganglia ; Parasympathetic postganglionic synapse ; Sympathetic postganglionic synapse ; Denervated bladder ; Acetylcholinesterase ; Catecholamine ; Histochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Histochemical techniques for acetylcholinesterase and catecholamine show that ureterovesical ganglia of both cat and dog contain dense intraganglionic cholinergic and adrenergic plexuses. Ramifications of both plexuses surround most cholinergic and adrenergic ganglion cell bodies as pericellular synaptic plexuses. Similar pericellular plexuses exist around extraganglionic cholinergic and adrenergic ganglion cells. Both adrenergic and cholinergic synaptic fibers persist in denervated pregnaglionic nerve-free specimens, indicating that cholinergic synaptic fibers are postganglionic parasympathetic in nature. The presence of adrenergic (postganglionic sympathetic) and postganglionic parasympathetic synapses around cell bodies in ureterovesical ganglia provides a morphologic basis for the sympathoinhibitory and muscarinic parasympatho-excitatory phenomena described in these ganglia.
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    URL: http://dx.doi.org/10.1007/BF00307343
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