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  • Spektralphotometrie  (51)
  • Cell types
  • Springer  (67)
  • Cambridge University Press
  • 1980-1984  (17)
  • 1970-1974  (50)
  • 1965-1969
  • 1955-1959
  • 1980  (17)
  • 1973  (24)
  • 1972  (26)
Collection
Keywords
Publisher
  • Springer  (67)
  • Cambridge University Press
Years
  • 1980-1984  (17)
  • 1970-1974  (50)
  • 1965-1969
  • 1955-1959
Year
  • 1
    Electronic Resource
    Electronic Resource
    Immersion and perfusion fixed rat adrenal medulla: The problem of mixed cells, clear cells, and the mode of secretion (1972)
    Springer
    Cell & tissue research 126 (1972), S. 261-277 
    Details
    ISSN: 1432-0878
    Keywords: Adrenal medulla ; Rat ; Cell types ; Secretion ; Influence of fixation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Das Nebennierenmark der Ratte wurde nach Anwendung verschiedener Fixationsmethoden untersucht. Nach Immersionsfixation mit Glutaraldehyd oder Osmiumtetroxyd finden sich in der Nebenniere sog. Mischzellen, helle Zellen, syncytiale oder plasmodiale Zellen, die von den Untersuchern als Artefaktbildungen angesehen werden. In allen Proben nach Perfusionsfixation wurden solche Artefaktbildungen niemals beobachtet. Bei Immersionsfixation wurde eine enge Beziehung zwischen Sekretgranula und Cytoplasmamembran beobachtet, was bei Perfusionsfixation selten der Fall war. Die heutigen Theorien über den Sekretionsmechanismus bei Nebennierenmarkzellen werden aufgrund der vorgelegten Ergebnisse diskutiert. Die Arbeit zeigt die wesentlichen Vorteile der Anwendung der Perfusionsfixation für die Untersuchung der Nebenniere.
    Notes: Summary The adrenal medulla of the rat was studied utilizing various methods of fixation. In adrenal medulla specimens after immersion fixation either with glutaraldehyde or osmium tetroxide, elements such as mixed, clear, syncytial, or plasmodial cells, believed to be of artifactual origin, are observed in all of this material examined. These elements are absent in the specimens prepared by perfusion fixation. In specimens prepared by immersion fixation, secretory granules are found in close proximity to the plasma membrane; this localization is infrequent after perfusion fixation. Current theories of the mechanism of secretion of adrenal medullary hormones are discussed on the basis of our results. This investigation demonstrates the advantage and necessity of perfusion fixation in the study of the adrenal medulla.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307220
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  • 2
    Electronic Resource
    Electronic Resource
    The ultrastructure of the adenohypophysis of Myxine glutinosa (1972)
    Springer
    Cell & tissue research 132 (1972), S. 451-472 
    Details
    ISSN: 1432-0878
    Keywords: Adenohypophysis ; Myxine glutinosa ; Cell types ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The ultrastructure of adenohypophysial cells in the Atlantic hagfish (Myxine glutinosa) is described and the morphological evidence for secretory activity is discussed. A scarcity of secretory granules is characteristic of the adenohypophysis of Myxine. Two cell types having the appearance of protein hormone producing cells can be identified. Type 1 has dense membrane-bound granules with a calculated mean diameter of 88 nm while type 2 has larger granules with a mean diameter of 176 nm. The release of secretory granular material follows mainly the “membrane-release” pattern. It is suggested that cell type 1 may produce a hormone which is similar to ACTH/MSH and type 2 another hormone similar to STH/LTH. The basophilic cells contain a secretory material which is similar to the mucus produced in the epithelial mucus cells. Several structural modifications are considered to represent functional compensations for the absence of vascular elements in the gland. Among these are a cytoplasmic tubular system, certain long agranular cells together with long granule-containing projections from cell types 1 and 2, and foliate or finger-like invaginations of the basal lamina.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00306636
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  • 3
    Electronic Resource
    Electronic Resource
    Ultrastructure of the pituitary gland (pars distalis) in sockeye salmon (Oncorhynchus nerka) during gonad maturation (1972)
    Springer
    Cell & tissue research 130 (1972), S. 338-350 
    Details
    ISSN: 1432-0878
    Keywords: Adenohypophysis ; Salmon ; Gonad maturation ; Cell types ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The fine structure of the various hormone-producing cell types (with the exclusion of the prolactin cells) in the pituitary gland (pars distalis) of migratory sockeye salmon is described. All fish were in an advanced stage of sexual maturation. In the proximal pars distalis five cell types were distinguished: growth hormone cells, ACTH cells, gonadotrops, “vesicular cells”, and “chromophobe cells”. Gonadotrops were also found throughout the rostral pars distalis. A conspicuous feature of the gonadotrops was the presence of two kinds of secretory inclusions: small electron-dense granules (200–375 mμ) and large, relatively electron-translucent globules (400–2 000 mμ). The large vesicular cells, so called because of their conspicuous vesicular endoplasmic reticulum, were numerous and often appeared to contain some small granules. It is argued that they may represent a second type of gonadotropic cell, which, in earlier stages of gonad development, contains many granules but becomes largely degranulated near the time of reproduction when the other gonadotrops (“globular gonadotrops”) abound. The chromophobes, which were smaller and far less abundant than the vesicular cells, also appeared to contain small granules (120–280 mμ). They are probably thyrotrops.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00306947
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  • 4
    Electronic Resource
    Electronic Resource
    Ultimobranchial gland ultrastructure of larval axolotls, Ambystoma mexicanum shaw, with some observations on the newt, Pleurodeles waltlii Micahelles (1972)
    Springer
    Cell & tissue research 134 (1972), S. 183-192 
    Details
    ISSN: 1432-0878
    Keywords: Ultimobranchial glands ; Urodeles ; Cell types ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The fine structure of the ultimobranchial (UB) glands of two common laboratory urodeles, viz., larval axolotls, Ambystoma mexicanum Shaw and adult Pleurodeles waltlii Micahelles, is described and compared in what is believed to be the first ultrastructural report on urodele UB glands. The axolotl UB gland shows a wide variety of form, being represented by an elongated diffuse series of follicles and sometimes by one or two large discrete terminal follicular bodies. In these axolotl UB glands up to four cell categories are distinguishable including a tonofilamentous cell and a secretory cell that is possibly homologous with calcitonin-producing C cells of anurans or other vertebrates. These two cell categories are also found in the Pleurodeles gland. The possible significance of the various cells is considered.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307152
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  • 5
    Electronic Resource
    Electronic Resource
    The outgrowth of rat cerebellum in organized culture (1973)
    Springer
    Cell & tissue research 146 (1973), S. 45-67 
    Details
    ISSN: 1432-0878
    Keywords: Cerebellum (rat) ; Growth in Tissue culture ; Cell types ; Light and electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The outgrowth of newborn rat cerebellum cultivated in the Maximow assembly was studied from the time of explanation until the end of the 1st week “in vitro”. Dynamic characteristics of migrating cells were investigated with time lapse microcinematography and with phase and interference contrast. Three types of cells were identified: bipolar fusiform cells, fibroblasts and macrophages. The ultrastructure of these cells as seen with scanning and transmission electron microscopes is described. Bipolar cells display the same characteristics as those of migrating granule cells “in vivo”. Differentiation of these cells towards neuronal types becomes evident at the end of the first week “in vitro”. Axonal as well as dendritic growth cones were examined, and their ultrastructural characteristics described. Attention is focussed into the abundance of smooth endoplasmic reticulum, which in supposed active growth has the form of thin cisternae, and, at rest or in retraction takes the form of large vesicles. In the latter case, filopodia disappear, and the tips of their processes acquire a bulbous shape.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00306758
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  • 6
    Electronic Resource
    Electronic Resource
    Les glandes exocrines des termites (1972)
    Springer
    Cell & tissue research 130 (1972), S. 205-218 
    Details
    ISSN: 1432-0878
    Keywords: Sternal gland ; Trinervitermes geminatus ; Cell types ; Histochemistry ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Résumé La glande sternale de Trinervitermes geminatus correspond à un épaississement médian de la partie antérieure du cinquième sternite abdominal. Elle est bordée intérieurement par une fine basale conjonctive et extérieurement par la cuticule, quelques sensilles campaniformes sont également visibles dans la masse glandulaire. On peut y reconnaître deux types de cellules: d'abord des cellules ovalaires situées dans la partie moyenne de la glande. Elles contiennent de grosses mitochondries et des globules denses, et sont en relation avec la basale par quelques fins prolongements cellulaires. Ensuite des cellules cylindriques, bien développées, constituant toute l'épaisseur de la glande. Elles possèdent de nombreuses vésicules de reticulum lisse et une bordure en brosse formée de microvillosités et de digitations cytoplasmiques qui pénètrent dans la cuticule. Cette cuticule comprend une mésocuticule lacunaire et une fine épicuticule percée par des «canalicules épicuticulaires». Une comparaison est établie avec la glande sternale d'autres termites, en particulier celle de Kalotermes.
    Notes: Summary The sternal gland of Trinervitermes geminatus appears as a median thickening of the epidermis at the anterior part of the fifth abdominal sternite. It is lined internally with a thin basal lamina and externally with the cuticle. A few campaniform sensilla are also visible in the glandular mass. Two types of cells may be recognized. First, oval-shaped cells, containing numerous large mitochondria and dense globules; these cells having no contact with the cuticle are connected with the basal lamina by a few narrow cytoplasmic stems. Second, tall columnar cells, with vesicular endoplasmic reticulum and an apical brush border built up by microvilli and finger—like processes which reach into the cuticle. This cuticle is made of alveolous mesocuticle and a thin epicuticle perforated by “epicuticular canals”. The organ is compared with the sternal gland of other termites, particularly with the one of Kalotermes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00306958
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  • 7
    Electronic Resource
    Electronic Resource
    Ultrastructure de la glande de la spermathèque chez Phosphuga atrata L. (Coleoptera Silphidae) (1972)
    Springer
    Cell & tissue research 128 (1972), S. 426-437 
    Details
    ISSN: 1432-0878
    Keywords: Spermathecal gland ; Silphidae ; Cell types ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Résumé Chez les Silphes et en particulier chez Phosphuga atrata, la glande de la spermathèque présente une structure particulière liée à la présence d'une intima cuticulaire tapissant la lumière de la glande. Elle comporte trois types cellulaires: les cellules sécrétrices, les cellules de l'épithélium sous-cuticulaire et les cellules-manchons. Les cellules sécrétrices de grande taille contiennent une invagination de la membrane cytoplasmique formant une «vacuole» extracellulaire bordée de microvillosités. Dans cette vacuole plonge l'extrémité, différenciée en ampoule poreuse, d'un canalicule de nature cuticulaire, qui véhicule la sécrétion jusqu'à la lumière de la glande. Le canalicule est élaboré par une cellule-manchon qui l'accompagne sur toute sa longueur sauf à son extrémité intravacuolaire. Ce type de glande, qui se retrouve chez de nombreux Insectes, y assurant des fonctions diverses (sécrétion odorifique, sécrétion de défense, sécrétion spermale, etc.), est susceptible de nombreuses variations.
    Notes: Summary The spermathecal accessory gland in the female of Phosphuga atrata (Silphidae), exhibits a special structure which is due to the presence of a cuticular intima lining the lumen. The wall of the gland shows three cellular types: the secretory cells, the epithelial cells and the ductule carrying cells. Each large secretory cell contains a cavity formed by an invagination of the cytoplasmic membrane and lined by many microvilli. The secretory cell is connected with a cuticular ductule ending in the cavity of the glandular cell, in a porous organelle. This ductule, which carries the secretory material to the lumen, is surrounded by the ductule carrying cell. This type of integumentary gland is very common in insects, where it assumes various functions (attraction, defense, conservation of sperm, etc.) and its morphology varies considerably.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00306980
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  • 8
    Electronic Resource
    Electronic Resource
    Golgi-Cox studies on the central nervous system of a gastropod mollusc (1972)
    Springer
    Cell & tissue research 128 (1972), S. 564-582 
    Details
    ISSN: 1432-0878
    Keywords: Brain ; Pond snail ; Neurons ; Cell types ; Golgi-Cox method
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Complete neurones were impregnated in the brain of the pulmonate gastropod pond snail, Lymnaea stagnalis L. using the Golgi-Cox method. Mapping of small to medium sized neurones identified in living preparations by the position of the perikarya was possible. Simple monopolar and bifurcating monopolar neurones with varying lateral patterns of short fine fibres were common in the pond snail brain. Larger neurones have more complex and numerous branches originating from axons close to the perikarya than smaller ones. Stem processes originating on the cell body were observed on neurones above 30 μ in somal diameter. Possible sites for the location of chemical synapses were suggested. Functional types of neurones were difficult to separate on morphological grounds. Giant or very large neurones are small in number in pond snail ganglia, compared with medium or small neurones.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00306989
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  • 9
    Electronic Resource
    Electronic Resource
    The fine structure of the statocyst of the prosobranch mollusc Pomacea paludosa (1972)
    Springer
    Cell & tissue research 133 (1972), S. 529-537 
    Details
    ISSN: 1432-0878
    Keywords: Statocyst ; Pomacea paludosa ; Ultrastructure ; Cell types ; Ciliary orientation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Ultrastructural observations on the statocysts of the prosobranch gastropod Pomacea paludosa are reported in this paper. 1. The static nerve consists of a great number of naked axons with a diameter of 0.2–0.5 μm. Several axons are joined together to bundles by glia cells (Fig. 1). 2. The epithelium of the statocyst contains 2500–3000 hair (= sense) cells, each with an area of at most 75 μm2 (Fig. 2). This great number of hair cells is compared with the number of receptor cells in other gastropod statocysts. 3. The hair cells are surrounded by smaller supporting cells. The significance of the interlacing of hair and supporting cells and of the possible contact between hair cells is discussed. 4. Supporting cells possess microvilli and sometimes one modified cilium (Fig. 5). The hair cells bear microvilli and 30–40 cilia. These cilia have the typical 9+2 arrangement of the filaments, striated roots to one side of the basal body and a basal foot to the other (Fig. 3). 5. For each sense cell and even for larger areas of the statocyst wall the basal feet point nearly in the same direction (Fig. 4). Hence it is suggested (a) a directional sensitivity for each hair cell and (b) a correlation between the areas of same polarisation and the “groups” of nerve fibers. 6. Different types of vesicles in the nerve layer of the cyst wall (Fig. 6) and stimulation experiments give some evidence for an efferent innervation of the statocysts.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307133
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  • 10
    Electronic Resource
    Electronic Resource
    Die Feinstruktur der Gallenblase und der Gallengänge (1972)
    Springer
    Cell & tissue research 135 (1972), S. 87-102 
    Details
    ISSN: 1432-0878
    Keywords: Gall bladder (Mouse) ; Epithelium ; Cell types ; Artifacts ; Light- and electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Die Feinstruktur des Epithels der Gallenblase der Maus wurde nach Fixation in vier verschiedenen Lösungen (OsO4 nach Palade, OsO4 nach Millonig, Glutaraldehyd und OsO4, Glutaraldehyd-Formaldehyd und OsO4) untersucht. Folgende Zellen sind unabhängig von der Fixierung erkennbar: 1. Hauptzellen, 2. Bürstenzellen, 3. Wanderzellen, 4. basalgekörnte Zellen. Die „hellen“ und „dunklen“ Hauptzellen sind jedoch als Fixationsartefakte zu betrachten. Die Erneuerung des Epithels und die funktionelle Bedeutung der vier verschiedenen Zelltypen werden diskutiert.
    Notes: Summary The fine structure of the gall bladder epithelium (mouse) has been investigated after fixation in four different solutions (OsO4-Palade, OsO4-Millonig, Glutaraldehyde and OsO4, Glutaraldehyde Formaldehyde and OsO4). Independent from fixation applied the following cells can be identified: 1. principal cells, 2. brush cells, 3. migrating cells, 4. granulated cells. The results show however that the “clear” and “dark” principal cells are to be considered as fixation artifacts. The renewal of the epithelium and the possible functions of the four cell types described are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307090
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