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  • Insects  (15)
  • Histochemistry  (7)
  • Springer  (22)
  • Institute of Physics
  • 1990-1994
  • 1970-1974  (22)
  • 1950-1954
  • 1971  (22)
Collection
Keywords
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  • Springer  (22)
  • Institute of Physics
Years
  • 1990-1994
  • 1970-1974  (22)
  • 1950-1954
Year
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Calcified tissue international 7 (1971), S. 267-276 
    ISSN: 1432-0827
    Keywords: Tech ; Development ; Enamel ; Enzyme ; Histochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Description / Table of Contents: Résumé L'activité en naphtylamidase est étudiéc au niveau des incisives et molaires de rat, à divers stades de développement. Du L-leucyl-4-methoxy-2-naphtylamide, du L-alanyl-4-methoxy-2-naphtylamide, du L-leucyl-2-naphthylamide et du DL-alanyl-2-naphtylamide sont utilisés comme substrats: du bleu rapide B et du grenat rapide GBC sont employés comme sels de diazonium. Le naphtylamidase n'est pas visible au niveau de dents, en voie de dévelopment, au cours de la formation matricielle de l'émail. A la fin de ce stade, le naphtylamidase est présent au niveau de l'extrémité distale des améloblastes, près de la surface de l'émail. L'activité enzymatique reste identique jusqu'au moment de la fusion de l'épithélium dentaire et de l'épithélium buccal, au moment de l'éruption de la dent dans la cavité buccale. On ne rencontre pas de naphtylamidase au niveau d'autres tissues dentaires; cependant une activité marquée est observée dans les ostéoclastes au niveau des surfaces de résorption de l'os alvéolaire, entourant les dents, en voie de développement et d'éruption, et dans certaines régions du tissu conjonctif.
    Abstract: Zusammenfassung Die Aktivität der Naphthylamidase wurde in den Backen- und Schneidezähnen von Ratten in verschiedenen Entwicklungsstufen studiert. Als Substrate wurden L-leucyl-4-methoxy-2-naphthylamid, L-alanyl-4-methoxy-2-naphthylamid, L-leucyl-2-naphthylamid und DL-alanyl-2-naphthylamid verwendet; als Diazoniumsalze dienten Echtblau B und Echt-Granat GBC. Naphthylamidase konnte während der Schmelzmatrixbildung im Zahn nicht nachgewiesen werden. Nach Abschluß dieser Phase erschien Naphthylamidase in den distalen Enden der Ameloblasten, nahe bei der Schmelzoberfläche. Die Enzymtätigkeit blieb am selben Ort lokalisiert, bis das Zahnepithel, im Augenblick wo der Zahn in die Mundhöhle durchstößt, in das Mundepithel überging. Naphthylamidase wurde in anderen Zahngeweben nicht gefunden, aber eine deutliche Aktivität konnte in gewissen Bezirken des Bindegewebes sowie in den Osteoklasten der resorbierenden Oberflächen vom alveolären Knochen festgestellt werden, welcher die sich bildenden und die hervorstoßenden Zähne umgibt.
    Notes: Abstract Naphthylamidase activity was studied in rat molar and incisor teeth at different stages of development. L-leucyl-4-methoxy-2-naphthylamide, L-alanyl-4-methoxy-2-naphthylamide, L-leucyl-2-naphthylamide and DL-alanyl-2-naphthylamide were used as substrates and Fast blue B and Fast Garnet GBC as diazonium salts. Naphthylamidase was not demonstrable in the teeth during enamel matrix formation. After the termination of this stage, naphthylamidase was present in the ameloblasts in their distal ends close to the enamel surface. The enzyme activity retained this localization until the dental epithelium fused with the oral epithelium at the time of tooth eruption into the oral cavity. Naphthylamidase was not found in other dental tissues, but marked activity was found in osteoclasts at the resorbing surfaces of alveolar bone surrounding the developing and erupting teeth and in certain areas of the connective tissue.
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  • 2
    Electronic Resource
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    Springer
    Cell & tissue research 113 (1971), S. 13-28 
    ISSN: 1432-0878
    Keywords: Sensory receptors ; Insects ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The antennae of Scolytus multistriatus were examined with light and scanning and transmitting-electron microscopy to determine the distributions, types and structures of sense organs. Four types of sensilla were found: (1) sensilla chaetica, a singly-innervated receptor; (2) sensilla basiconica, Type A, a short, thin-walled, multiple-innervated receptor; (3) sensilla basiconica, Type B, a long, thin-walled multiple-innervated receptor; and (4) sensilla trichodea, short, thick-walled, multiple-innervated receptors. The positioning of dendrite(s) with regard to pore tubules is elaborated. Evidence for lack of sensory axon fusion is presented.
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  • 3
    Electronic Resource
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    Springer
    Cell & tissue research 112 (1971), S. 15-30 
    ISSN: 1432-0878
    Keywords: Slug ; Biogenic amines ; Histochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The distribution of monoamines inLimax maximus was studied by the histochemical fluorescent method of Falck and Hillarp. The number of 5-HT-containing and catecholamine-containing perikarya in the central nervous system is small compared with the non-fluorescent perikarya. However, all the ganglia except the proto-cerebral ganglia have some amine-containing neurons. There are relatively larger numbers of fluorescent cells in the cerebral, visceral, pedal and right parietal ganglia than in the other ganglia. A single, giant 5-HT-containing neuron was observed in each meta-cerebral ganglion. Monoamine neurons are localised in a number of peripheral tissues (heart, integument, tentacles, penis retractor muscle, sole of foot, kidney, alimentary canal, reproductive organs and tentacular, pharyngeal and cephalic retractor muscles). Neurons containing catecholamine are mostly associated with sensory structures such as the statocysts, the retina of the eye and the integument of the tentacles, whereas 5-HT-containing nerve fibres are mainly observed in muscle tissues.
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  • 4
    Electronic Resource
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    Springer
    Cell & tissue research 112 (1971), S. 97-119 
    ISSN: 1432-0878
    Keywords: Insects ; Epicuticle secretion ; Golgi system ; Lysosomes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Résumé Les modifications cytologiques des cellules épidermiques sécrétant la cuticule sternale et pleurale de l'adulte deT. molitor sont étudiées pendant la formation et l'évolution de l'épicuticule externe et interne. L'épicuticule externe et interne sont sécrétées de la même façon par condensation de matériel diffus au-dessus de l'apex des microvillosités. Un cycle hétérolytique est mis en évidence par l'emploi de peroxydase injectée dans l'espace exuvial: la peroxydase est captée par de grosses vésicules revêtues et incorporée dans des corps multivésiculaires apicaux. Par comptage des différentes populations de vésicules, des variations nettes dans l'intensité de la pinocytose sont mises en évidence. Ces variations semblent en rapport non pas avec la digestion de l'ancienne cuticule mais avec les différences de nature et de structure des composants cuticulaires. L'hypothèse du contrôle de la sécrétion épidermique par les phénomènes de pinocytose énoncée par Locke (1969) est confirmée. Des vésicules d'origine golgiennes sont secrétées surtout après la formation de l'épicuticule et ne sont donc pas à l'origine du matériel épicuticulaire, mais il est possible qu'elles jouent un rôle dans l'évolution ultérieure des structures épicuticulaires.
    Notes: Summary The cytological modifications of the epidermal cells secreting sternal and pleural cuticle of adultTenebrio are studied, specially during the formation and evolution of the outer and inner epicuticle. Both layers are secreted by condensation of diffuse material just above the epidermal microvilli. There is no peculiar phase without microvilli when inner epicuticle is formed. During the secretion of pre-ecdysial cuticle, the epidermal cell is characterized by an heterolytic process which is demonstrated by peroxidase injection into the ecdysial space: horseradish peroxidase is absorbed by large pinocytic coated vesicles which discharge their content into multivesicular bodies, after the loss of their coat. The intensity of pinocytosis is studied by vesicles counts at different stages of the pupal life. These counts show a pinocytic peak when the inner epicuticle is formed. When the epicuticular barrier is completely secreted, exogenous peroxidase is no more absorbed, but morphological study and counts show that pinocytosis still occurs. This demonstrates that epidermis cannot absorb macromolecules arising from the digested old cuticle since digestion occurs after the completion of adult epicuticle. Locke's hypothesis about the “control of the extracellular subcuticular environment” by pinocytosis is confirmed. The Golgi complexes are involved in lytic process with their small coated vesicles and in secretion of cuticular material with their large dense secretory vesicles, but according to vesicle counts, the secretory vesicles are not concerned in epicuticle formation since their apparition is posterior to the deposition of the inner epicuticle.
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  • 5
    Electronic Resource
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    Springer
    Cell & tissue research 114 (1971), S. 61-72 
    ISSN: 1432-0878
    Keywords: Corpora cardiaca ; Neurosecretion ; Insects ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Résumé Les corpora cardiaca de l'adulte de Locusta migratoria sont formés de deux régions bien individualisées ce qui nous a permis de reconnaître la sécrétion propre des différents types de neurosécrétion. Dans la région nerveuse, nous distinguons par la taille des grains trois types de neurosécrétion dense classique et un quatrième type d'aspect clair. Dans la région »propre« non nerveuse, les cellules ont des caractères nettement endocriniens et sont mélangées à un seul type d'axones neurosécréteurs.
    Notes: Summary The corpora cardiaca of adult Locusta migratoria consist of two well separated areas, a fact which permits the differentiation between intrinsic and extrinsic neurosecretory material. In the neural area three types of electron dense “classical” neurosecretory granules, and a fourth more lucent type can be distinguished according to size. In the non-neural “glandular” area typical endocrine cells mingle with only one type of neurosecretory axons.
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  • 6
    Electronic Resource
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    Springer
    Cell & tissue research 116 (1971), S. 405-424 
    ISSN: 1432-0878
    Keywords: Midgut ; Formica ; Histophysiology ; Histochemistry ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Résumé Les cellules du mésentéron des ouvrières, des reines et des mâles de Formica polyctena F. possèdent un certain nombre de particularités cytologiques dont l'évolution a été suivie au cours du développement post-embryonnaire et du cycle annuel. A l'apex des cellules de régénération les microvillosités se différencient avant l'élimination des cellules caduques larvaires ou nymphales. A partir de la nymphose une activité sécrétoire apocrine se manifeste dans la partie dorsale de l'épithélium du mésentéron, l'ensemble des cellules assurant par ailleurs la fonction absorbante de l'organe. Il existe deux sortes d'inclusions cytoplasmiques, des polysaccharides et des concrétions minérales. Les polysaccharides sont surtout abondants chez les larves et les nymphes: le glycogène, polysaccharide de réserve, est utilisé au cours de l'histogénèse; des mucopolysaccharides acides, d'origine golgienne, représentent une sécrétion muqueuse. Les sphérocristaux sont constitués de strates concentriques de phosphates et chlorures de calcium et d'une matrice de mucopolysaccharides. La cristallisation des éléments minéraux s'effectue, à partir de la nymphose seulement, dans les citernes ergastoplasmiques. Cette accumulation d'ions pourrait être en relation avec le régime alimentaire de l'insecte ou représenter une voie d'excrétion.
    Notes: Summary The midgut cells of workers, queens and males of the ant Formica polyctena show cytological characteristics which were studied in the course of postembryonic development and annual cycle. The microvilli of the regenerating cells appear before the elimination of the regressing larval and pupal cells. At the time of pupation, an active phase of apocrine secretion begins in the dorsal part of the midgut epithelium, while the absorptive function is carried out by all cells of the organ. Two types of cytoplasmic inclusions coexist: polysaccharides and mineral concretions. The polysaccharides are particulary abundant in larvae and pupae. Glycogen is metabolized during histogenesis; acid mucopolysaccharides, elaborated in the Golgi apparatus, represent a mucous secretion. The spherites are composed of concentric strata of calcium phosphate and chloride and a matrix of mucopolysaccharides. These minerals form in the ergastoplasmic cisternae of pupal cells only. Their accumulation could be related to the insect's diet, or it could reflect a process of excretion.
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  • 7
    ISSN: 1432-0878
    Keywords: Notochord ; Caecilians ; Histochemistry ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The notochord of Ichthyophis glutinosus and I. kohtaoensis consists of peripheral flattened cells characterized by a well-developed system of rough endoplasmic reticulum, bundles of tonofilaments, and abundant glycogen particles. These cells contain furthermore fairly high activities of α-naphtyl-acetate esterase and 4-chloro-5-bromoindoxyl acetate esterase as well as acid phosphatase which was found in lysosomal localization. The huge intracellular vacuoles of the centrally situated cells possibly originate from electron translucent spaces within the glycogen fields of the peripheral cells. The notochord sheath consists of variously differentiated layers of collagen fibers and of an elastica externa. The diameters of the collagen fibers increase from the inner towards the outer region of the sheath. A peculiar feature of the Ichthyophis notochord sheath is a ring of mineralized collagen. The notochord of the caecilians investigated is compared with that of anurans, urodeles, and several groups of fish.
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  • 8
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    Cell & tissue research 118 (1971), S. 333-368 
    ISSN: 1432-0878
    Keywords: Malpighian tubule ; Insects ; Carausius ; Microscopy, electron ; Biological transport
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Structural features of the principal, urine-secreting cells (type 1 cells) of the Malpighian tubules of Carausius are de scribedquantitatively and discussed in relation to possible mechanisms of water and solute transport. Mitochondria are arranged in two bands of about equal volume near to the basal and apical surfaces, suggesting active processes occur at both surfaces. Basal infoldings and apical microvilli which greatly amplify the cell surface are probably primarily devices to increase the passive permeability of the tissue to solutes. They do not provide functionally significant standing-osmotic-gradients. The extensive endoplasmic reticulum is locally differentiated into several components and ramifies between the infoldings and along microvilli but probably is not an intracellular conduit for the majority of urinary constituents. Vesicles and stages in their formation or liberation are observed both basally and apically although they probably do not contribute significantly to transcellular transport. At present it remains a problem to satisfactorily account for observations that the urine of Carausius can be hypotonic.
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  • 9
    ISSN: 1432-0878
    Keywords: Chordotonal organ ; Insects ; Head appendages ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Résumé Les organes chordotonaux présents dans les différentes pièces céphaliques de la larve du Speophyes peuvent être classés en deux catégories. La première catégorie regroupe les récepteurs scolopidiaux de l'antenne, du labium et du palpe maxillaire. On peut les comparer au scolopidium de l'organe tympanique du Criquet décrit par Gray (1960). La deuxième catégorie comprend les récepteurs scolopidiaux de la mandibule et de la lacinia: ils sont du amphinématique. Le sensille scolopidial de la galea représente un type intermédiaire. Nous signalons l'importance des structures de soutien et de fixation, qui doivent permettre une bonne transmission de toutes les déformations et tensions subies par le tégument. Nous discutons du rôle joué par la ≪gap junction≫ qui unit les deux dendrites dans les scolopidium. de la deuxième catégorie. Enfin nous essayons d'établir des hypothèses sur le fonctionnement des scolopidium.
    Notes: Summary The chordotonal organs located in the various head appendages of the Speophyes larva, can be divided into two classes. The scolopidial receptors of the antenna, the labium and the palpus maxillae belong to the first class. They can be compared to the scolopidium of the locust tympanic organ described by Gray.—The second class contains the scolopidial receptors of the mandible and the lacinia: their type is amphinematic. The scolopidial sensilla of the galea represents an intermediate type. We demonstrate many supporting and fixation structures which probably allow a good transmission of all the deformations and strains affecting the tegument. The function of the gap junction which connects the two dendrites in the scolopidia of the second class is discussed. Finally we try to formulate hypothesis of the functioning of scolopidia.
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  • 10
    ISSN: 1432-0878
    Keywords: Symbiotes ; Hereditary ; Insects ; Types ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Résumé L'examen au microscope électronique des symbiontes transmis par l'œuf chez les Homoptères, montre des types très divers: levures, bactéries plus ou moins régressées et des formes énigmatiques ressemblant parfois à des globules de sécrétion. Toutefois, les diverses catégories de symbiontes décrites auparavant en microscopie photonique ne sont pas toujours retrouvées. Il n'est pas possible de caractériser un genre par ses symbiontes.
    Notes: Summary Electron microscope study of symbiotes hereditarily transmitted through the eggs of in Homoptera, shows very different types: yeasts, more or less degenerated bacteria and enigmatical forms looking in some way like drops of secretion. Yet the numerous distinct species of symbiotes described in some cases by light microscopists are not always found again. It is not possible to distinguish a genus by its symbiotes.
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  • 11
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    Springer
    Cell & tissue research 122 (1971), S. 210-226 
    ISSN: 1432-0878
    Keywords: Insects ; Cercus ; Mechanoreceptors ; Fine structur ; Ecdysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung 1. Mit einsetzender Apolysis öffnet sich der äußere Liquorraum der Sensillen in den Exuvialraum. Die cuticularen Scheiden und die in ihnen enthaltenen Ciliarstrukturen werden verlängert. Die cuticularen Scheiden grenzen die Sinnescilien gegen die Exuvialflüssigkeit ab. 2. Über die cuticularen Scheiden stehen die alten rezeptorischen Apparate mit den Sinneszellen bis zur Ecdysis in Verbindung. Bei der Bildung der neuen Cuticula wird das Cuticulamaterial um sie herum abgelagert. Dadurch entstehen Kanäle, die wir Häutungskanäle nennen. Sie bleiben auch nach der Ecdysis erhalten, wenn die cuticularen Scheiden und die Sinnescilien hier abgerissen sind. 3. Der Häutungskanal der Faden- und Keulenhaare liegt über der Haarbasis, der der campaniformen Sensillen in der Mitte der cuticularen Kuppel. 4. Im Sinnescilium wird bereits vor der Ecdysis unter der neuen Cuticula ein zweiter, neuer Tubularkörper gebildet. Der alte Tubularkörper geht bei der Häutung verloren. Dadurch können die Sensillen wahrscheinlich bis zur Häutung (alter Tubularkörper!) und sofort nach der Häutung (neuer Tubularkörper!) Reize wahrnehmen. 5. Mechanorezeptorische Sinneshaare und campaniforme Sensillen sind homologe Bildungen. Die innere Hüllzelle (H 1) legt die cuticulare Scheide an. Die mittlere Hüllzelle (H 2) ist die trichogene und die äußere Hüllzelle (H 3) die tormogene Zelle.
    Notes: Summary 1. With the onset of apolysis the external extracellular space of the sensilla opens into the exuvial space. The cuticular sheaths and the ciliary structures become lengthened. The cuticular sheaths delimit and protect the modified cilia against the exuvial fluid. 2. The old receptor apparatus stays in contact with the sensory cells above the cuticular sheath until ecdysis. In the formation of the new cuticle, the cuticular material is deposited around the cuticular sheath, thereby forming channels, called the ecdysial canals. These also remain intact after the cuticular sheath and the modified cilium become torn away. 3. The ecdysial canal of the filiform and club-shaped hairs is situated above the base of the hair; the ecdysial canal of the campaniform sensilla is in the middle of the cuticular cap. 4. A second and new tubular body is already formed under the new cuticle in the modified cilium before ecdysis. The old tubular body disappears during ecdysis. The sensilla therefore can probably perceive stimuli until the time of ecdysis through the old tubular body and there-after through the new tubular body. 5. Mechanoreceptive sensory hairs and campaniform sensilla are homologous structures. The internal enveloping cell (H 1) lays down the cuticular sheath. The middle enveloping cell (H 2) is the trichogen cell, and the external one (H 3) is the tormogen cell.
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  • 12
    ISSN: 1432-0878
    Keywords: Insects ; Sensory Apparatus ; Mandible ; Speophyes lucidulus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Résumé L'organisation sensorielle de la mandibule de la larve duSpeophyes lucidulus est étudiée au microscope électronique. L'équipement sensoriel est remarquablement homogène puisqu'il ne comprend que des mécanorécepteurs: — récepteurs tactiles, comme les sensilles trichoïdes du bord externe de la mandibule, — propriocepteurs, comme les sensilles campaniformes d'une part et les récepteurs scolopidiaux d'autre part, qui sont les plus abondants. Ceux-ci se terminent dans les zones de la mandibule qui sont le plus fortement soumises à des tensions et des déformations lors des mouvements mandibulaires. Ces récepteurs présentent en outre certaines caractéristiques morphologiques originales que nous décrivons.
    Notes: Summary The sensorial organization of the mandible of theSpeophyes lucidulus larva is studied by means of electron microscopy. This sensorial apparatus is remarkably homogeneous, i.e. it includes only mechanoreceptors: — tactile receptors such as trichoide sensillae in the external edge of the mandible, — proprioceptors such as campaniform sensillae and scolopidia which are the more numerous. The latter, end in the zone of the mandible which are widely concerned with strains and deformations during mandibular movements. Furthermore, these receptors present certain original morphological features which are described below.
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  • 13
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    Cell & tissue research 113 (1971), S. 361-387 
    ISSN: 1432-0878
    Keywords: Insects ; Molt ; Proctodeum ; Cuticle
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Résumé La formation des divers types de cuticule rectale a été suivie au cours de la mue chezKalotermes flavicollis (Isoptera). Dans l'épithélium rectal, l'épicuticule externe est formée de couches alternativement sombres et claires, comme dans les autres types de cuticule; l'épicuticule interne présente d'abord une fine lamellation de sa région superficielle, lamellation qui disparaît ensuite. Sur les papilles rectales, l'épicuticule externe a d'abord le même aspect, mais ensuite des différences apparaissent dans l'évolution des différentes couches; ces différences sont encore plus accentuées dans les dépressions épicuticulaires, où les couches superficielles de l'épicuticule externe disparaissent. Sur le cadre bordant les papilles, le dépôt de l'épicuticule externe est peu différent de ce qu'on observe sur l'épithelium rectal; l'épicuticule interne, très mince, présente là aussi une lamellation temporaire; le reste de la cuticule acquiert d'emblée la structure homogène observée pendant l'intermue. Dans tous les cas, la sécrétion de la cuticule (y compris la sous-cuticule) est terminée au moment de l'exuviation. Les modifications des cellules sont brièvement décrites; les cellules des papilles rectales subissent une dédifférenciation complète de leur surface apicale, mais, sur les faces latérales, les associations des mitochondries avec les replis des membranes plasmiques subsistent en grande partie.
    Notes: Summary The formation of the different kinds of rectal cuticle has been followed during the molt inKalotermes flavicollis (Isoptera). In the rectal epithelium, the outer epicuticle consists of alternating dark and light layers, as previously described in other types of cuticle; the inner epicuticle first exhibits in its superficial part a thin multilayered pattern, which later disappears. In the rectal papillae, the outer epicuticle has primarily the same appearance, but the evolution of the various layers is somewhat different; even more pronounced differences occur in the epicuticular depressions where the more superficial layers of the outer epicuticle disappear. In the border of the rectal papillae, the outer epicuticle is laid down according nearly the same way as in the rectal epithelium; the temporary multilayered pattern of the very thin inner epicuticle also may be observed; the underlying part of the cuticle, as soon as it is formed, shows the homogeneous appearance observed during the intermolt. In all the cases, the secretion of the cuticle (sub-cuticle included) is achieved when the ecdysis occurs. The modifications affecting the cells are briefly described; the apical surface of the rectal papillae cells undergoes a complete dedifferentiation, but, on the lateral faces, the associations of mitochondria with plasma membrane interdigitations for a large part remain unchanged.
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  • 14
    ISSN: 1432-0878
    Keywords: Acrosomal cap formation ; Mammals ; Histochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary By applying the periodic acid-Schiff technique to frozen sections of material fixed in weak Bouin's solution and extracted with hot pyridine, the contributions of the acrosomal vesicle and granule to the acrosomal cap have been observed in the maturing spermatozoa of the American opossum, guinea pig, rabbit, marmoset and chimpanzee. In the spermatids of the opossum, the acrosomal granule is not developed and the thin, homogeneous carbohydrate layer of the acrosomal cap is derived from the concentrated material of the acrosomal vacuole. In the guinea pig, both the acrosomal vesicle and granule make the greatest contributions to the carbohydrates of the acrosomal cap; these continue to maintain their identity in the head of mature sperm. In the rabbit, marmoset and chimpanzee, the carbohydrate material of acrosomal vesicle origin is less developed and constitutes a thin layer in the lateral portions of the acrosomal cap; the carbohydrate material in its anterior portions is mainly derived from the acrosomal granule which becomes flattened; there is also a slight thickening of the intermediate layer of the acrosomal cap in this region. The distinction between the carbohydrate contributions from the acrosomal vesicle and granule disappears in the fully formed acrosomal cap which shows a homogeneously PAS-positive intermediate layer. In the chimpanzee, the acrosomal vesicle makes a relatively very small contribution to the carbohydrate layer of the acrosomal cap which is mainly formed by spreading of material from the acrosomal granule.
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  • 15
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    Cell & tissue research 120 (1971), S. 1-16 
    ISSN: 1432-0878
    Keywords: Corpus allatum ; Insects ; Cribriform inclusions ; Ultrastructure ; Cytophysiology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Ultrastructural evidence is presented for the manufacture, storage, and release of a distinctive cellular product in the corpus allatum of the insect Leucophaea. This product (C body material) originates in the Golgi zone and acquires a characteristic, regularly structured appearance of exceptionally high electron density. The differential distribution of this easily identified product in various sites in the organ permits the reconstruction of a sequence of dynamic events involving transport from the intra- to the extracellular compartment which affords access to the circulation. A small amount of (surplus ?) C body material becomes incorporated in multivesicular bodies. The variability in the occurrence, distribution, and fate of the C body material during various periods of the animal's life cycle, and under experimentally altered conditions, suggests a relationship with the glandular function of the corpus allatum. Conspicuous deposits of C body material, especially in the extracellular stroma, parallel situations in which the hormonal activity of the organ seems to be low or temporarily suspended. Thus the ultrastructural manifestations of intermittent sluggishness in the system, as observed in Leucophaea, provide valuable cues for the existence of a periodically changing production line involving a specific glandular material. The precise relationship with the comparable cyclicity of juvenile hormone activity remains to be ascertained.
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  • 16
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    Cell & tissue research 117 (1971), S. 394-418 
    ISSN: 1432-0878
    Keywords: Portal Vein ; Innervation ; Histochemistry ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Die V. portae der weißen Ratte wurde licht-, fluoreszenz- und elektronenmikroskopisch auf ihre Innervation untersucht. 1. Paraldehydbedampfte Venenpräparate und Häutchenpräparate der gesamten Wandung (Falcksche Fluoreszenzmethode) lassen einen überwiegend längsorientierten äuβeren Nervenplexus erkennen, der den äußersten Muskelzellen aufliegt. Er ist leberseitig weitmaschig, darmseitig sehr engmaschig. Ein subendothelial gelegener innerer Plexus ist vorwiegend zirkulär orientiert. Er entspringt dem äußeren Plexus der darmseitigen Gefäßpartien. 2. Der Nachweis der Acethylcholinesterase (Gomori-Methode) bringt lichtmikroskopisch einige Nervenbündel in der bindegewebigen Adventitia zur Darstellung. Im übrigen findet sich die Aktivität des Enzyms nur in den interzellulären Spalten der Muskelschicht. Der elektronenmikroskopische Nachweis der Acethylcholinesterase (Karnovsky-Methode) läßt aber erkennen, daß sich die Enzymaktivität auf die Muskelzellmembranen beschränkt. 3. Die elektronenmikroskopische Untersuchung bestätigt den fluoreszenzmikroskopischen Befund. a) Lebernah finden sich nur vereinzelte Axonbündel, die der äußeren Muskellage aufgelagert sind. Die Einzelaxone sind vollständig von den Schwannschen Zellen umgeben. Nur wenige, den Muskelzellen benachbarte Axone enthalten agranuläre Vesikel. Sehr selten sind Ausfaltungen der vesikelhaltigen Axone zu sehen, deren Abstand zur Muskelzelle aber immer noch 1000–2000 Å beträgt. b) Auf über eintausend Dünnschnitten wurde kein Axon innerhalb der dicken Muskelschicht gefunden. c) Subendothelial verlaufende Axone (innerer Plexus) sind teilweise oder völlig aus den Schwannschen Zellen ausgefaltet. Sie sind dicht besetzt mit leeren Vesikeln (300–650 Å) und enthalten wenige kernhaltige Vesikel in der Größenordnung 800–1600 Å. Synaptische Endigungen werden nicht beobachtet. d) Eine dichte Häufung vesikelhaltiger Axone, die teils völlig, teils nur an der muskelzellnahen Seite aus den Schwannschen Zellen ausgefaltet sind, finden sich am Übergang der V. mesenterica superior zum Pfortaderstamm, deren einschichtiger Muskellage angelagert. Von diesen Bündeln stammende kleinere Bündel und Einzelaxone ziehen zwischen den Muskelzellen hindurch und erreichen das Endothel. Typische Synapsen werden nicht beobachtet. Kein vesikelhaltiges Axon nähert sich mehr als 1000 Å den Muskelzellen. 4. Die ausgefalteten vesikelbesetzten Axone werden als vegetative Überträgerstrecken angesehen. Die Erregung der Effektorstrukturen durch Transmittersubstanzen wird im Zusammenhang mit der postmortalen autonomen Gefäßkontraktilität diskutiert.
    Notes: Summary The innervation of the portal vein of the white rat was examined with light-, fluorescence-, and electronmicroscopic techniques. The results are as follows: 1. Paraldehyde treated vein preparations (Falck's fluorescence method) demonstrate a predominantly longitudinally orientated external nerve plexus, being situated on the outermost muscle cells. Near the liver the nerve net is characterized by broad meshes, near the intestinal tract by narrow ones. The circular subendothelial inner plexus originates in the outer plexus of the intestinal vascular bed. 2. Nerve bundles in the fibrous adventitia were demonstrated with Gomori's Acethylcholinesterase method. In other respects, the enzyme activity was only observed in the intercellular spaces of the muscle layer. The electronmicroscopic demonstration of Acetylcholinesterase (Karnovsky's method) further showed that the enzyme activity is restricted to the muscle cell membrane. 3. The electronmicroscopic examination verified the results obtained with fluorescence microscopic techniques. a) In the proximity of the liver, only isolated nerve bundles occur on the outer muscle layer. The individual nerves are entirely surrounded by Schwann cells. Only a few of the axons in the vicinity to the muscle cell have agranular vesicles. Evaginations of the vesicular axons occur infrequently. Their distance from the muscle cell amounts to 1000–2000 Å. b) In more than one thousand thin sections, no axons were found inside the thick muscular layer. c) Subendothelial axons (inner plexus) are either partially or totally evaginated from the Schwann cells. They are densely filled with empty vesicles (350–650 Å) and contain a few dense core vesicles of 800–1600 Å in diameter. Synaptic endings were not observed. d) A dense collection of vesicle-containing axons, that were partially in their entirety and partially only from the muscle cell proximal side evaginated from the Schwann cells, were observed in the single muscle layer at the junction of the superior mesenteric and the portal vein. From these bundles, smaller bundles and individual axons pass between the muscle cells and reach the endothelium. Typical synapses were not observed. No vesiclecontaining axon was nearer than 1000 Å to the muscle cell. 4. Those axons possessing vesicles and being evaginated are considered to be vegetative conducting pathways. The excitation of the effector structures by transmitter substances is discussed in connection with the post mortem autonomic vascular contractility.
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  • 17
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    Cell & tissue research 117 (1971), S. 463-475 
    ISSN: 1432-0878
    Keywords: Bone marrow ; Leukocytes ; Electron microscopy ; Histochemistry ; Peroxidase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The ultrastructural localization of peroxidase activity has been studied in the cells of normal human bone marrow using the diaminobenzidine peroxidase technique. Peroxidase activity has been localized within the primary (azurophil) granules of the neutrophilic series as well as in the cytoplasmic granules of eosinophils, basophils and monocytes. Peroxidase activity appears within the cisternal system (nuclear envelope, Golgi complex and rough endoplasmic reticulum) of these cells during the period of peroxidase-containing lysosome production. With the cessation of granulogenesis, peroxidase activity disappears from the cisternal system and does not reappear in subsequent developmental stages. In cells incubated in peroxide-free media, staining of granular components, but not of cisternae, is reduced. The inclusion of catalase in peroxide-free media eliminates all staining. This indicates that an endogenous peroxide is present within the cisternae and granules of these cell types.
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  • 18
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    Cell & tissue research 117 (1971), S. 570-575 
    ISSN: 1432-0878
    Keywords: Olfactory receptors ; Insects ; Stimulus conduction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Bei den olfaktorischen Sensilla trichodea des Seidenspinners wird die Cuticula des Sinneshaares von zahlreichen Poren durchbrochen. Es wird gezeigt, daß diese Poren mit den Dendriten der Sinneszellen durch extrazelluläre Porentubuli verbunden sind. Daraus folgt, daß die Duftmoleküle den Ort der Erregungsbildung an der Rezeptormembran wahrscheinlich allein durch Oberflächendiffusion erreichen, ohne eine dreidimensionale Diffusion durch den Sensillenliquor.
    Notes: Summary In the olfactory sensilla trichodea of the silk moth, the cuticle of the sense hair is perforated by numerous pores. These pores are shown to be connected with the dendrites of the receptor cell by extracellular pore tubules. It is suggested, therefore, that odour molecules reach the stimulus transduction sites on the receptor membrane by surface diffusion alone, and need not diffuse three-dimensionally through the sensillum liquor.
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  • 19
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    Cell & tissue research 117 (1971), S. 537-558 
    ISSN: 1432-0878
    Keywords: Nervous System ; Insects ; Trichoptera ; Monoamine containing structures
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Mit Hilfe der fluoreszenzmikroskopischen Methode nach Falck und Hillarp wurden die monoaminhaltigen Strukturen im Zentralnervensystem einiger Trichopteren untersucht (vgl. Klemm, 1968). Im Protocerebrum können vier Gruppen von katecholamin-haltigen Zellkörpern unterschieden werden. Eine weitere unregelmäßig darstellbare Gruppe von globulösen Perikarya liegt im Lobus opticus. Fluoreszierende Varikositäten durchsetzen locker das Cerebralganglion und sind in folgenden Neuropilstrukturen konzentriert: Medulla, Lobula, Corpus centrale, Nodulus, Corpus ventrale, α- und β-Lobus und Lobus communis. Letzterer verbindet den α- und den β-Lobus mit dem nicht fluoreszierenden Pedunculus. In der Lamina, in der accessorischen Medulla, in der Pons cerebralis, im Tuberculum opticum, im Tractus olfactorio-globularis, im Pedunculus, im Stielglomerulus und in den Globulizellen ließen sich keine Katecholamine nachweisen. Zwischen dem monoaminhaltigen Lobus communis und dem nicht fluoreszierenden Pedunculus besteht eine scharfe Grenze. Fluoreszierende variköse Fasern und einzelne fluoreszierende Perikarya befinden sich im Deuto- und Tritocerebrum. Zwei fluoreszierende Bahnen können im Cerebralganglion unterschieden werden: 1. Stratum caudale, 2. Tractus ventralis. Letzterer beginnt mit seiner Pars anterior und posterior im dorsalen fluoreszierenden Neuropil des Protocerebrum. Die beiden Teile laufen frontal und caudal um den Zentralkörper herum und vereinigen sich unterhalb des Zentralkörpers in der Pars communis. Von hier aus zieht der paarige Tractus ventralis bis in das Tritocerebrum. Die Frage einer Homologisierung dieser Bahnen wird diskutiert. Ähnlich wie im Ganglion suboesophageale sind in den drei thorakalen und fünf separaten abdominalen Ganglien zwei Paar fluoreszierender Zellkörper vorhanden, in denen Dopamin mikrospektrofluorimetrisch festgestellt werden konnte. Ein Paar dieser Zellen liegt caudoventral, das andere dorso-median bis dorso-caudal. Ihre Zellfortsätze und Abzweigungen werden beschrieben. In einzelnen Fällen konnte noch ein zweites dorsales Paar fluoreszierender Perikarya sichtbar gemacht werden. Das letzte Ganglion der Bauchkette setzt sich aus zwei bis drei Gangliomeren zusammen. Die Anzahl ihrer fluoreszierenden Perikarya ist reduziert. Das Neuropil der Ganglien im Bauchmark ist von monoaminhaltigen Fasern durchsetzt, wobei sich in der dorsalen Hälfte mehr fluoreszierendes Neuropil befindet als in der ventralen. Lateral in den Ganglien sind die monoaminhaltigen varikösen Fasern vorwiegend dorsoventrad angeordnet. Im medianen Teil laufen sie in Längsrichtung, verzweigen sich und setzen sich in die Konnektive fort und verbinden die katecholaminhaltigen Neuropilbereiche der einzelnen Ganglien miteinander.
    Notes: Summary The distribution of monoamine-containing structures in the central nervous system of Trichoptera has been investigated with the histochemical fluorescence method of Falck and Hillarp (cf. also Klemm, 1968). In the protocerebrum, four groups of catecholamine containing perikarya can be distinguished. Another group of irregularly appearing cell bodies is situated in the optical lobe. Fluorescent varicosities occur scattered throughout the cerebral ganglion, being concentrated in the following neuropile areas: In the medulla, the lobula, the corpus centrale, the noduli, the corpora ventralia, and the α- and β-lobes, as well as in the lobus communis where the α- and β-lobes join the non-fluorescent pedunculus. In the lamina, the accessory medulla, the pons cerebralis, the tuberculum opticum, the tractus olfactorio-globularis, the pedunculus including its glomerular region, and the globuli-cells, no catecholamines are detectable. There is a sharp borderline between the monoamine-containing lobus communis and the non-fluorescent pedunculus. Fluorescent varicose fibres and single fluorescent perikarya occur in the Deuto- and Tritocerebrum. Two fluorescent tracts can be distinguished in the cerebral ganglion: 1. Stratum caudale. 2. Tractus ventralis. The pars anterior and pars posterior of the tractus ventralis begin in the dorsal fluorescent neuropile of the cerebral ganglion and join underneath the central body into their pars communis. From this, it can be traced as tractus ventralis into the tritocerebrum. The possible homologies of these tracts are discussed. Similar to the ganglion suboesophageale, the three thoracal and the five separate abdominal ganglia contain two paires of fluorescent cell bodies. Microspectrofluorometrically a content of dopamine in these cells could be established. One pair lies caudoventrally. The two other cell bodies are situated dorso-medially to dorso-caudally; their position varies notably, especially in the abdominal ganglia. The cell process and its arborisations are described. Occasionally a second dorsal cellpair could be observed. The last ganglion of the abdominal chain is composed of at least two gangliomeres with a reduced number of fluorescent perikarya. The neuropile of the thoracal- and abdominal ganglia is penetrated by monoamine-containing fibres, with a predominance in the dorsal half. In the ganglia, the fluorescent varicose fibres are mainly oriented dorso-ventrally in the lateral part and longitudinally in the medial part, where they branch and continue into the connectives. In this way, the catecholamine-containing neuropiles of adjacent ganglia are connected to each other.
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  • 20
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    Cell & tissue research 117 (1971), S. 419-442 
    ISSN: 1432-0878
    Keywords: Taste hairs ; Insects ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Die Schmeckhaare der Fliege Phormia terraenovae enthalten fünf Rezeptorzellen (vier Kontaktchemo- und eine Mechanorezeptorzelle), die von zwei Hüllzellen unter Aussparung von Lymphräumen („Vakuole“) umgeben sind. Die Rezeptorzellen gliedern sich in Axon, Zellkörper und Dendrit, der aus Innenglied, Übergangsabschnitt und Außenglied besteht. Der Übergangsabschnitt hat die 9+0-Struktur der modifizierten Cilien mit zwei Basalkörpern. Im Plasma der Außenglieder sind nur Mikrotubuli zu erkennen. Die Außenglieder der Kontaktchemorezeptoren ziehen durch die röhrenförmige Dendritenscheide und den Kanal I des Haarschafts bis zu einem Porus in der Haarspitze, durch den sie mit den Schmeckstoffen in Kontakt kommen (Ort der Primärprozesse). Das Außenglied der Mechanorezeptorzelle enthält einen tubulären Körper und setzt an der Haarbasis an. Die Hüllzelle II (tormogene Zelle) bildet zum großen Sensillenlymphraum hin ein stark gefaltetes Membransystem aus. Der Feinbau wird in Beziehung zur Funktion der Schmeckhaare gesetzt.
    Notes: Summary The tarsal taste hairs of the blowfly contain five receptor cells: four contact chemoreceptors and one mechanoreceptor. These cells are surrounded concentrically by two sheath cells with fluid-filled spaces (“vacuoles”) between them. The receptor cell parts are the axon, the cell body, and the dendrite. The latter has an inner segment, a transitional region, and an outer segment. The transitional region shows the 9+0 configuration of a modified cilium with two basal bodies. In the outer segment only microtubules are found. The outer segments of contact chemoreceptors pass through the tube-like dendrite sheath, then through “canal I” of the hair shaft, to reach the pore at the tip of the hair. The outer segment of the mechanoreceptor contains a “tubular body” and terminates at the hair base. The “sheath cell II” (tormogene cell) forms a folded membrane system toward the main liquor space. The fine structure of the tarsal taste hairs is discussed in relation to their function.
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  • 21
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    Cell & tissue research 120 (1971), S. 309-320 
    ISSN: 1432-0878
    Keywords: Oocytes ; Ribonucleic acid synthesis ; Insects ; Autoradiography
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A large DNA containing body is found in oocytes of the house cricket, Acheta domesticus. Little or no RNA synthesis is associated with the DNA body during the leptotene, zygotene, and pachytene stages of meiotic prophase I. During the early diplotene stage of development, large masses of nucleolar material begin to accumulate at the periphery of the DNA body. The onset of RNA synthesis correlates with a change in the histochemically detectable histone proteins associated with the DNA body. In ovaries of animals injected with uridine-H3, most of the label accumulates in ribosomal RNA. Autoradiographic studies show that the cytoplasm of late diplotene stage cells accumulates uridine label to a greater extent than does the cytoplasm of early diplotene stage cells. Increased transport of nucleolar material through the nuclear envelope of late diplotene stage cells accounts for the increased cytoplasmic labeling.
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  • 22
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    Cell & tissue research 121 (1971), S. 442-454 
    ISSN: 1432-0878
    Keywords: Visual system ; Insects ; First optic chiasma ; Fibre projection ; Light microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Each optic cartridge in the lamina of Diptera1 gives rise to a bundle of fibres composed of the prolongations of at least 11 neurons (two first order receptors, R7 and R8, five monopolar cells, L1–L5 and four “centrifugal” cells, T1, T1a, C2 and C3). The bundles project to the outer surface of the second synaptic region, the medulla. The projection patterns of the bundles means that a point for point map of the cartridge arrangement in the lamina is conferred on the medulla. The cross-over of bundles, along the horizontal axis of the eye, merely reverses the lamina map on the medulla. All eleven fibres that enter a bundle at the lamina are contained within it as far as the medulla.
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