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  • Development  (14)
  • Springer  (14)
  • Blackwell Publishing Ltd
  • Nature Publishing Group
  • 1975-1979
  • 1970-1974  (14)
  • 1970  (14)
Collection
Publisher
  • Springer  (14)
  • Blackwell Publishing Ltd
  • Nature Publishing Group
Years
  • 1975-1979
  • 1970-1974  (14)
Year
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 108 (1970), S. 380-396 
    ISSN: 1432-0878
    Keywords: Epicuticle ; Insects ; Tenebrio molitor ; Development ; Structure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Résumé L'épicuticule de l'adulte de Tenebrio molitor est composée de deux couches distinctes dénommées épicuticule externe et épicuticule interne. L'épicuticule externe est la première couche cuticulaire sécrétée sous forme de petites plaques s'agrandissant par leurs bords pour recouvrir toute la surface cellulaire. Au moment de sa sécrétion, cette couche est formée de quatre lames denses A, B, C1 et C2. La lame B, très fine, disparaît par la suite et les lames C1 et C2 deviennent très nettes. L'épicuticule externe de l'adulte est donc formée de trois lames denses séparées par deux lames claires. L'épicuticule interne est formée de lames superposées denses et claires de structure complexe, qui sont masquées pendant la sécrétion des premières couches de cuticule lamellée (procuticule). Cette structure correspond à un arrangement moléculaire hautement organisé. La forme de la surface cuticulaire des sternites est déterminée par la forme de la surface cellulaire avant le dépôt de l'épicuticule.
    Notes: Summary The epicuticle of adult Tenebrio consists of two distinct layers named outer and inner epicuticle. The outer epicuticle is the first cuticular layer to be deposited in form of small patches on top of the microvilli. These initial patches are composed of four dense laminae (A, B, C1 and C2) separated by three light spaces. The outer epicuticle grows by densification of diffuse material at the edges of the patches until the entire area is covered. The thickness of outer epicuticle remains constant (175 Å) during the development of the pharate adult, lamina B however rapidly disappears. Thus, the adult outer epicuticle is fivelayered (three dense laminae: A, C1 and C2). After being deposited, the inner epicuticle shows a complex laminar structure interpreted to represent a highly organized molecular system. The laminae are masked during the formation of the first procuticle lamellae. During the deposition of the epicuticle, lamina A is covered by a component of the moulting fluid, forming an irregular dense layer which disappears after the resorption of this fluid. Perhaps this layer protects the new epicuticle from lytic enzymes of the moulting fluid. In adult animals, there is an additional superficial layer, the signification of which is not clear. The possibility of remains of cement or wax is discussed. The development of the surface patterns of the sternal and pleural cuticle is determined before the epicuticle formation by the shape of the epidermal surface. The rate of outer epicuticle deposition appears to depend on the size of the microvilli: epicuticle deposition seems to proceed faster over high microvilli.
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 109 (1970), S. 1-14 
    ISSN: 1432-0878
    Keywords: Seminal vesicle ; Prostate ; Fetal rat ; Ultrastructure ; Development
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The development of the seminal vesicle from the Wolffian duct and of the prostate from the urogenital sinus has been studied in rat fetuses from day 14 of gestation to birth with the use of the electron microscope. Prior to the onset of androgen secretion, the cells of the urogenital sinus and the caudal part of the Wolffian duct have a simple undifferentiated appearance. After the onset of androgen secretion by the fetal testes at day 15, “intracytoplasmic confronting cisternae” of the granular reticulum appear in both urogenital sinus and Wolffian duct. Portions of the granular endoplasmic reticulum of the urogenital sinus become distended with a finely granular, moderately dense material. In the urogenital sinus, many hemidesmosomes are formed at the basal surface of the epithelium. Specializations of the extracellular materials are present opposite the hemidesmosomes. The formation of the seminal vesicles and the prostate begins at day 18–19 of gestation. The cells of the seminal vesicle are taller than the Wolffian duct cells from which they arise, the granular endoplasmic reticulum increases moderately in amount, and a patent lumen is formed. The cells of the fetal prostate do not differ greatly from those of the urogenital sinus from which they arise except that the prostatic cells initially lack hemidesmosomes. The fine structural changes are discussed in relation to the onset of fetal androgen secretion, the formation of the organs, and the functions of the cells in adult life.
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 111 (1970), S. 346-363 
    ISSN: 1432-0878
    Keywords: Kidney ; Glomerulus ; Development ; Scanning electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Die Differenzierung der Podocyten wurde an Nieren 10 Tage alter Ratten raster-elektronenmikroskopisch untersucht und mit durchstrahlungs-elektronenmikroskopischen Befunden verglichen. Die Podocytenfortsätze können danach auf dreierlei Wegen gebildet werden: 1. Spalten innerhalb des Cytoplasmas lassen bandartige Cytoplasmabrücken entstehen. Diese gliedern sich weiter auf, bis zahlreiche miteinander verzahnte Fortsätze derselben Zelle entstanden sind. 2. Vom Zellrand her werden dicke Fortsätze weit vorgeschoben, die kleinere Fortsätze bilden. Durch sie können Verzahnungen mit entfernten Deckzellen entstehen. Die kleinen Fortsätze können sich jedoch auch mit anderen Fortsätzen der eigenen Zelle verzahnen. 3. Fingerförmige Fortsätze benachbarter Zellen verzahnen sich während ihrer Entstehung miteinander. Trotz zahlreicher desmosomenartiger Haftstellen zwischen benachbarten Podocyten entwickeln sich ihre Fortsätze und deren Verzahnungen anscheinend weitgehend autonom und nur selten nach den vermuteten Regeln epithelialer Nachbarschaft (Typ 3). Die Befunde sprechen vielmehr dafür, daß durchflutete und wachsende Glomerulumkapillaren die Podocytendifferenzierung induzieren und die Orientierung der Fortsätze beeinflussen.
    Notes: Summary The differentiation of the podocytes was studied by scanning electron microscopy on kidneys of 10 days old rats. The results were compared with transmission electron microscopic pictures from the same kidneys. There are three ways of forming processes by the podocytes: 1. Slits within the cytoplasm give rise to cytoplasmic bridges which further divide themselves and finally build up a meshwork of processes within a cell. 2. Thick and sometimes very long processes originate from the cell border. Their smaller branches may interdigitate with those of distant podocytes or with other processes out of the same cell. 3. Finger-like processes of neighbouring cells interdigitate as soon as they develop. In spite of numerous desmosomal structures between neighbouring podocytes the cell processes and their interdigitations develop mostly independently from each other and only seldom after the expected rules of epithelial vicinity (type 3). These findings are interpreted as indication that flooded and growing capillaries induce the differentiation of podocytes and that they influence the orientation of their processes.
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 106 (1970), S. 348-370 
    ISSN: 1432-0878
    Keywords: Ovary ; Insect ; Chironomus ; Development ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Im Ovar von Chironomus sind in Phase 1 des 4. Larvenstadiums polygonal abgeflachte „Innenzellen“ von kleineren „Außenzellen“ umgeben, die Bakteroide und Phagosomen enthalten; zwischen den Innenzellen liegen unregelmäßige Zelltrümmer („keimbahnbegleitende Substanzen“). Zu Beginn der Ovariolenbildung werden in Phase 3 durch Spalträume zwei Schichten der Außenzellen voneinander getrennt, von denen die innere (Follikel- und Eikanalepithel) regelmäßige Buchten bildet. In diese Buchten wandern von innen Zellpaare ein, die an „synaptischen Komplexen“ bzw. multiplen Chromatinstrukturen als Ei- und Nährzellen kenntlich sind. Zwischen beiden Zellen sind „Fusome“ häufig, die später in eigentümlicher Weise geschlossen werden. Zwischen den Eikanalzellen entsteht in Phase 5 durch Spaltbildung der Eikanal; in Phase 7 sind die Eikanalzellen auffallend glykogenreich. Kurz vor der Vitellogenese treten im Bereich der Oocyte Membransysteme und „annulated lamellae“ auf; akzessorische Kerne werden als Ausstülpungen des Oocytenkernes gebildet und später abgeschnürt. In Phase 9 sind an der Peripherie der Eizelle Mikrovillisäume und Pinocytosebläschen sichtbar. Die distalen Zellen der Ovariole haben Eioder Nährzellcharakter, sind aber bei Ch. melanotus nicht von Follikelzellen umgeben und werden beim weiteren Ovariolenwachstum reduziert. Trotz extrem geringer Nährzellzahl der Follikel scheint das Chironomus-Ovar funktionell nicht von anderen polytroph meroistischen Insektenovarien unterschieden.
    Notes: Summary In the ovary of Chironomus during phase 1 of the fourth larval instar, polygonally flattened “inner cells” are surrounded by smaller “outer cells” which contain bacteroids and phagosomes. Irregular cell remnants (“germ line accompanying substances”) lie among the inner cells. At the beginning of ovariole formation in phase 3, two layers of outer cells are separated by the formation of fissures. The inner layer of these cells (follicle- and egg-passage epithelium) forms regular invaginations. Cell pairs, identified as oocytes and nurse cells by “synaptic complexes” or multiple chromatin structures, wander from inside into the invaginations. Frequently between the two cells are fusomes, which later close in a characteristic manner. During phase 5, an egg passage is formed as a fissure among the egg-passage cells. During phase 7, the egg passage cells are conspicuously full of glycogen. Shortly before vitellogenesis membrane systems and annulated lamellae appear in the region of the oocyte. Accessory nuclei are formed by a “tieing-off” of projections of the the oocyte nucleus. During phase 9, microvilli and pinocytotic vesicles can be seen at the periphery of the oocyte. The distal cells of the ovariole are of oocyte or nurse cell nature, but in Ch. melanotus they are not surrounded by follicle cells and are reduced during further ovariole growth. In spite of the extremely small number of nurse cells in the follicle, the Chironomus ovary apparently does not differ functionally from other polytrophic meroistic insect ovaries.
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 109 (1970), S. 138-146 
    ISSN: 1432-0878
    Keywords: Insect muscles ; Development ; Microtubuli ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung In der indirekten Flugmuskulatur von Phormia terrae-novae kann die Entwicklung der Myofibrillen deutlich in eine Anlage- und eine Wachstums-Phase unterteilt werden. Zu Beginn der Wachstumsphase wurde Puppen eine Lösung von Colchicin in die rechte Metathoraxhälfte injiziert. Als Folge dieser Behandlung lösten sich zunächst die Mikrotubuli in der Flugmuskulatur auf. In späteren Entwicklungsstadien bildeten sich atypische Verzweigungen der Myofibrillen, die zu einer partiellen Desorientierung der kontraktilen Strukturen führten. Ein Mechanismus, der solche Störungen des Orientierungmusters in der Normalentwicklung möglicherweise verhindert, wird diskutiert.
    Notes: Summary In the blowfly Phormia terrae-novae the development of myofibrils of indirect flight muscles can be divided into periods of predisposition (anlage) and of growth. At the beginning of the growth period microtubules are disrupted by injection of colchicine. This disruption is followed by the formation of atypical ramifications of myofibrils at Z-discs leading to numerous disoriented myofibrils in late developmental stages. A possible mechanism preventing these alterations during normal development is discussed.
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 110 (1970), S. 72-84 
    ISSN: 1432-0878
    Keywords: Lung cells ; Chick embryo ; Lamellar inclusions ; Ultrastructure ; Development
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Résumé Les pneumocytes granuleux, qui constituent l'un des principaux types cellulaires de l'épithélium pulmonaire, sont caractérisés par la présence de volumineuses inclusions osmiophiles lamellaires. Nous avons étudié l'apparition et l'origine de ces inclusions dans l'épithélium du poumon embryonnaire de Poulet, en l'examinant à différents stades du développement. Les premières inclusions lamellaires apparaissent dans le poumon de l'embryon de 16 jours. A ce stade, quelques lamelles concentriques entourent une zône centrale amorphe étendue; la périphérie des inclusions contient toujours de petites structures granulaires. Les jours suivants le nombre de cellules contenant des inclusions lamellaires augmente rapidement; en même temps, les lamelles deviennent plus nombreuses. A 19 jours, les inclusions lamellaires ont un aspect semblable à celui qu'elles ont dans les poumons d'animaux adultes. Dès l'apparition des ébauches pulmonaires, à 2 1/2 jours d'incubation, les cellules épithéliales contiennent des inclusions typiques: les inclusions granulaires. Ces organites sont caractérisés par un centre granulaire, qu'entouré un système membranaire. Ce système, simple chez le jeune embryon, évolue ensuite en se compliquant; chez l'embryon de 16 jours, il s'enroule en plusieurs couches autour de la masse centrale. Au moment où les premières inclusions lamellaires apparaissent, le nombre des inclusions granulaires augmente rapidement; on les trouve souvent étroitement associées à des vacuoles lipidiques. L'analyse des relations entre inclusions lamellaires, inclusions granulaires et vacuoles lipidiques suggère que l'inclusion lamellaire résulte de la collaboration entre une vacuole lipidique et plusieurs inclusions granulaires.
    Notes: Summary The granular pneumocytes, one of the main cellular types of the lung epithelium, are characterized by the presence of large osmiophilic lamellar inclusions. The appearance and origin of these inclusions has been studied in the epithelium of chick embryonic lung at different developmental stages. Lamellar inclusions are first seen in the lung of 16 day old embryos. At this stage, few concentric lamellae surround a large amorphous center; the periphery of the inclusions always contains small granular structures. In the following days, the number of cells containing these lamellar inclusions increases rapidly, while their lamellae progressively become more numerous. In 19 day old embryos, the lamellar inclusions are similar to those in the lungs of adult animals. From the earliest formation of the bronchial primordia, their epithelial cells contain a number of typical “granular” inclusions. These organelles are characterized by a granular center, enclosed in a membranous system. This structure becomes more complex as the embryo develops; in the 16 day old embryo, the multilayered membranous system coils around the granular center. At the time when lamellar inclusions first appear, granular inclusions increase rapidly in number and are often found in close association with lipidic vacuoles. The relationships between lamellar inclusions, granular inclusions and lipidic vacuoles are discussed. The evidence suggests that a lamellar inclusion arises from the cooperation of several granular inclusions and a lipidic vacuole.
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 110 (1970), S. 173-184 
    ISSN: 1432-0878
    Keywords: Submandibular gland ; Secretory units ; Development
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The fine structure of the secretory units of the mouse submandibular gland was studied according to the developmental sequence. The embryonic submandibular gland consists of terminal tubules and ducts. Myoepithelium is associated only with the terminal tubules, and the cells of the primary intercalated ducts show characteristics of the young striated duct cells. The major changes shortly after birth consist of: 1) opening of the secretory lumina, 2) increasing rough ER and its altered configuration, 3) dilatation of Golgi cisternae and 4) changes in the granular structure. These findings suggest that the salivary secretion first occurs after birth, and acinar differentiation or transformation of the secretory cells of the terminal tubules is induced and profoundly affected by the commencement of the secretory activity. In the intercalated ducts this process is somehow inhibited, and the granular cells found in the adult can be considered as the remnants of the secretory cells of the terminal tubules.
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 110 (1970), S. 401-443 
    ISSN: 1432-0878
    Keywords: Integument ; Oenocytes ; Structure ; Development ; Culex pipiens
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung 1. Struktur und Entwicklung des Integuments und der larvalen Oenocyten während des Puppen- und pharaten Imaginalstadiums von Culex pipiens L. (Dipt.) werden beschrieben. 2. Auf- und Abbau der pupalen und die Anlage der imaginalen Cuticula verlaufen innerhalb enger zeitlicher Grenzen. 3. Beim Abbau der pupalen Endocuticula erscheint immer die Lamina der jeweils innersten Lamelle als „Häutungsmembran“. 4. Die Oenocyten sind durch ihr agranuläres, tubuläres endoplasmatisches Retikulum (ATER) und dessen Veränderungen charakterisiert. Die Struktur der Oenocyten gleicht der steroidproduzierender Wirbeltierzellen. 5. Das Aussehen der Oenocyten ändert sich kurz nach der Puppenhäutung hauptsächlich durch die kontinuierliche Zunahme von sog. Lipidvesikeln. Diese Vesikel entstehen durch Erweiterungen tubulärer Elemente des agranulären endoplasmatischen Retikulums. 6. Im Lichtmikroskop erscheinen diese Veränderungen als Kanalstrukturen („Sekretionsphase“) kurz nach der Puppenhäutung; später nimmt das Cytoplasma eine mehr vakuoläre Struktur an („Degenerationsphase“). 7. Mit Sudanschwarz B und Sudan III/IV lassen sich Lipide in den Erweiterungen der Tubuli des agranulären endoplasmatischen Retikulums (= Lipidvesikel) nachweisen. Nach Osmiumsäure-Fixierung sind sie nicht darstellbar. 8. Kurz vor der Imaginalhäutung kommt es in den Oenocyten zu massiver Autophagie. Dieser folgt ein Abbau des ATER und anderer Teile des Cytoplasmas. 9. Es gibt keinen Hinweis, daß die Oenocyten direkt für die Bildung der epicuticularen Lipide verantwortlich sind.
    Notes: Summary 1. Structure and development of the integument and the larval oenocytes during the pupal and pharate imaginai stage of Culex pipiens L. (Dipt.) are described. 2. Formation and digestion of the pupal cuticle and the formation of the imaginai cuticle take place within temporarily fixed limits. 3. While the pupal endocuticle is digested the lamina of the at times innermost lamella appears as a “ecdysial membrane”. 4. The oenocytes are characterized by their agranular, tubular endoplasmic reticulum (ATER) and its changes. The structure of the oenocytes is comparable to that of vertebrate cells engaged in steroid hormone synthesis. 5. The morphology of the oenocytes shortly after pupal ecdysis changes mainly by the continual raising of so called lipid vesicles. These vesicles arise from dilatations of tubular elements of the agranular endoplasmic reticulum. 6. Studied under the light microscope these changes appear as channel structures (“secretory phase”) shortly after pupal ecdysis; later on the cytoplasma becomes vacuolar in structure (“degeneration phase”). 7. By staining with Sudan black B and Sudan III/IV lipids can be demonstrated in dilatations of the tubules of the agranular endoplasmic reticulum. However they do not stain after fixation with osmium tetroxide. 8. In the oenocytes massive autophagy takes place just before imaginai ecdysis. It is followed by breakdown of the ATER and other parts of the cytoplasma. 9. There is no evidence that the oenocytes are directly responsible for the formation of epicuticular lipids.
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 105 (1970), S. 33-54 
    ISSN: 1432-0878
    Keywords: Microsporidian ; Development ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Vegetative growth of Nosema sp. occurs within the gut submucosal cells of Callinectes sapidus. Vegetative cell morphology is dominated by profiles of endoplasmic reticulum, numerous free ribosomes and aggregates of vesicles enclosed by a membranous sac. The dikaryotic vegetative cell is the earliest stage found in the target area for sporogenesis, the sarcoplasm of the striated muscle cell. The next obvious stage is the sporoblast mother cell; it undergoes karyokinesis without breakdown of the nuclear envelope. Intranuclear mitotic microtubules extend from the chromosomes to the intact nuclear envelope. After repeated nuclear divisions, the sporoblast mother cell undergoes delayed cytokinesis and a series of sporoblast progeny develops. The polar filament is the first visually apparent system to develop during sporogenesis. It appears to be of dual origin: (1) the central core component is condensed in Golgi-like saccules, and (2) the envelopes around the core originate from the endoplasmic reticulum. The polaroplast, which forms after early polar filament development, appears to originate as an elaboration of the endoplasmic reticulum.
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 104 (1970), S. 19-28 
    ISSN: 1432-0878
    Keywords: Pineal complex ; Development ; Aves, Columbidae ; Accessory evaginations ; Pineal tract
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Study of the pineal complex of several Columbiformes demonstrates: a) the presence, in embryos, of numerous evaginations of the diencephalic roof; b) the presence, in adults, of a parenchymal accessory structure, probably a remnant of one or more secondary evaginations; c) the variability, among specimens, of the diencephalic course of the pineal tract fibers; d) the predominant asymmetry of the basal attachment of the pineal stalk to the left of the mid-line.
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