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  • Cytochemistry  (7)
  • Springer  (7)
  • American Chemical Society
  • 1980-1984
  • 1970-1974  (7)
  • 1970  (7)
Collection
Publisher
  • Springer  (7)
  • American Chemical Society
Years
  • 1980-1984
  • 1970-1974  (7)
Year
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 108 (1970), S. 309-323 
    ISSN: 1432-0878
    Keywords: Vagal Paraganglia ; Catecholamines ; Ultrastructure ; Radioautography ; Cytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Typical vagal paraganglia of Syrian hamsters are encapsulated in connective tissue and consist of groups of epithelial cells. Ganglion cells, a few fenestrated capillaries, and bundles of unmyelinated nerve fibers are intermingled among the parenchymal cells. The parenchymal cells are of two types: chief or paraganglion and sustentacular or supporting cells. The processes of the supporting cells partly or completely surround the paraganglion cells. In addition to the nucleus, Golgi complex, mitochondria, parallel-arrayed granular endoplasmic reticulum, and lipofuscin pigment, the chief cells are characterized by the presence of numerous membrane-bound, electron opaque granules. After an injection of 3H-dopa, labelings were concentrated over the chief cells and were associated predominantly with the granules. Following glutaraldehyde-dichromate treatment the granules gave a positive reaction for unsubstituted amines. These results suggest that the chief cells contain catecholamines in the electron opaque granules.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 107 (1970), S. 249-264 
    ISSN: 1432-0878
    Keywords: Reptiles ; Germ cells ; Cytology ; Cytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Résumé Les gonocytes primaires des Reptiles sont très riches en granules vitellins et en liposomes cytoplasmiques; ils contiennent aussi un peu de glycogène. Les mitochondries se groupent dans une zone juxtanucléaire. La répartition de la chromatine est diffuse et chez certains Reptiles le nucléole des gonocytes est annulaire; une plage nucléaire particulière que nous avons dénommée «masse paranucléolaire» est visible à proximité de ce nucléole. L'évolution de ces différents constituants cellulaires est suivie au cours du développement embryonnaire et après la naissance. Les caractères cytologiques mis en évidence permettent de reconnaître sans ambiguïté les gonocytes dès les jeunes stades du développement embryonnaire. La «masse paranucléolaire» permet d'établir une continuité directe de la lignée germinale, sur le plan cytologique, depuis la fin de la gastrulation jusque chez l'adulte.
    Notes: Summary The primary gonocytes contain numerous yolk granules and lipid droplets; there is also some glycogen in their cytoplasm. Mitochondria are located in a juxtanuclear area. The chromatin is diffuse and in some reptiles a ring-shaped nucleolus is noted. Near the nucleolus there is a peculiar area which we have called “masse paranucléolaire”. The fate of all these cellular components was studied throughout embryonic development and during postnatal life. These cytologic characteristics permit the identification of the gonocytes from the gastrula stage. The “masse paranucléolaire” permits the clear demonstration of a continuity of the germinal line from gastrulation to the adult stage.
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 111 (1970), S. 379-403 
    ISSN: 1432-0878
    Keywords: Subcommissural organ ; Rana temporaria ; Ultrastructure ; Cytochemistry ; Cyto-enzymology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Most of the subcommissural organ (SCO) cells are in contact with the ventricle of the brain as well as with the limiting membranes: the ependymal cells. They are able to release their aldehyde-fuchsin (AF) positive secretory substance directly into the ventricle. A small number of cells, which also contains AF positive material, is not in contact with the ventricle: the hypendymal cells. The secretory substance in the cells is a mucopolysaccharide-protein complex, not containing strong acidic groups. It is released into the brain ventricle and condenses to a well-developed Reissner's fibre. Energy production in the cells mainly takes place by means of anaerobic glycolysis. The metabolism is characteristic of synthesis and secretion of a mucopolysaccharide-protein complex. These functions can be accomplished in rather anaerobic conditions. The cells contain two distinct compartments of endoplasmic reticulum, one regularly studded with ribosomes, situated perinuclearly and supranuclearly with the primary function of synthesizing and another, very irregularly studded with ribosomes, located mainly subnuclearly, with the primary function of storing secretory substance. No signs of release of secretory material into blood vessels or at the outer limiting membrane have been observed. Synaptic endings may be observed on the basal parts of the cells. Biogenic monoamines could not be demonstrated in the area of the SCO.
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 108 (1970), S. 135-149 
    ISSN: 1432-0878
    Keywords: Microbodies ; Peroxisomes ; Cytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Microbodies (peroxisomes), a group of cytoplasmic organelles enriched in catalase, are demonstrated in the toad, Bufo marinus, by light and electron microscopy by means of a cytochemical staining procedure that demonstrates the peroxidatic activity of catalase with diaminobenzidine (DAB). Amphibian microbodies are similar to those of other classes in their fine structure and localization in hepatocytes and kidney, where they are prominent in the proximal tubular cells. Nucleoids are present only in renal microbodies. In the proximal renal tubule an unusual group of large brown granules are identified as lysosomes by their acid phosphatase, β-glucosaminidase and β-glucuronidase activities.
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 104 (1970), S. 390-404 
    ISSN: 1432-0878
    Keywords: Polychetes ; Cerebral hormone ; Oogenesis ; Cytochemistry ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Résumé En l'absence d'hormone cérébrale, les ovocytes de N. pelagica subissent un accroissement rapide de taille. Ils présentent dans les grandes lignes une évolution cytologique parallèle à celle de l'ovogenèse naturelle. Ces ovocytes acquièrent en particulier des mucopolysaccharides acides qui se répartissent en fin d'évolution en une gangue corticale. Ce matériel toutefois est moins abondant que dans le cas de l'ovogenèse naturelle. A l'échelle de la microscopie électronique, diverses particularités caractérisent les ovocytes ayant évolué en condition anhormonale. Elles sont d'autant plus notables que le diamètre initial des ovocytes en expérimentation est plus faible. On note en particulier un accroissement des formations de reticulum et une augmentation considérable du nombre et de l'importance des lamelles annelées cytoplasmiques et intra-nucléaires. Enfin, la structure des lobules vitellins se caractérise par la présence de formations vésiculaires et lamellaires. La signification de ces modifications ultrastructurales est discutée.
    Notes: Summary In the absence of brain hormone, the oocytes of N. pelagica undergo a fast growth. They show a cytologic development parallel to that of natural oogenesis. In particular, these oocytes acquire acid mucopolysaccharides which finally form a cortical layer. However, this material is less abundant than in natural oogenesis. Electron microscopic investigation reveals some pecularities in oocytes growing under these abnormal conditions. The smaller the initial diameter of the oocytes, the more pronounced are these special traits. Most evident is an increase of the endoplasmic reticulum and a large augmentation in the number of cytoplasmic and intranuclear annulate lamellae. Moreover, the structure of the yolk bodies shows characteristic vesicular and membranous formations. The significance of these ultrastructural modifications is discussed.
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 103 (1970), S. 341-350 
    ISSN: 1432-0878
    Keywords: Catecholamines ; Brain ; Insects ; Electron microscopy ; Cytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In the brain of the cockroach Periplaneta americana, the beta lobes of the corpora pedunculata respond with an intense positive reaction to a specific fluorescence histochemical method for catecholamines. The fluorescence reaction disappears completely after prolonged treatment of the cockroaches with reserpine. An ultrastructural examination of the beta lobes in formaldehyde-glutaraldehyde-osmium fixed preparations reveals the presence of two types of fibres: 1) Fibres and nerve endings containing small clear vesicles and sligthly larger vesicles with a semi-dense content. The appearance and size distribution of these vesicles ist not affected by treatment with reserpine. 2) Fibres containing larger and denser vesicles, but practically no clear vesicles. The size distribution of these dense vesicles is only slightly affected by treatment of the cockroaches with reserpine. If brain slices are incubated in a medium containing noradrenaline or α-methyl-noradrenaline and fixed in permanganate, small vesicles with electron-dense central cores show up, similar to those which have been described in vertebrate adrenergic nerve fibres (“small granular vesicles”). They are confined to one of the two types of fibres (a and b) visible in these preparations, namely to type b, whose correspondence with type 2 fibres of formaldehyde-glutaraldehyde-osmium fixed preparations is discussed.
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 104 (1970), S. 375-389 
    ISSN: 1432-0878
    Keywords: Oocyte development ; Polychetes ; Cytochemistry ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Résumé Les ovocytes de N. pelagica d'un diamètre inférieur à 100 μ ne renferment dans leur cytoplasme que des inclusions lipidiques et vitellines. Les lobules vitellins s'accroissent par l'adjonction de vésicules golgiennes. L'approche de la maturité sexuelle est caractérisée par l'apparition de mucopolysaccharides acides. Corrélativement à l'élaboration de ce matériel, l'appareil de Golgi présente, à ce stade, de profondes modifications morphologiques: dilatation des saccules distaux et libération dans le cytoplasme de vacuoles golgiennes. Les processus de vitellogenèse cependant ne sont pas entièrement stoppés. Dans les ovocytes matures, les lobules mucopolysaccharidiques forment une gangue corticale. Les dictyosomes sont refoulés vers l'intérieur du cytoplasme et présentent des figures d'involution. La présence de lamelles annelées intra-nucléaires a été observée de façon constante dans tous les ovocytes matures examinés.
    Notes: Summary The oocytes of N. pelagica, the diameter of which is smaller than 100 μ, contains only lipid and yolk inclusions in their cytoplasm. The yolk bodies grow by confluence of Golgi vesicles. The advent of the sexual maturity is marked by the appearance of acid mucopolysaccharides. Concomitantly with the production of this material, the Golgi apparatus shows typical morphological modifications: dilatation of the distal saccules and release of Golgi vacuoles into the cytoplasm. However vitellogenesis is not completely terminated at this point. In the mature oocytes the mucopolysaccharid material forms a cortical layer. The dictyosomes are pushed toward the center of the cytoplasm and show signs of degeneration. The presence of intranuclear annulate lamellae is a constant feature in all mature oocytes examined.
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