ALBERT

All Library Books, journals and Electronic Records Telegrafenberg

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
Filter
  • Electron microscopy  (20)
  • Innervation  (10)
  • Springer  (29)
  • 2020-2024
  • 1990-1994
  • 1970-1974  (29)
  • 1970  (29)
Collection
Keywords
Publisher
  • Springer  (29)
Years
  • 2020-2024
  • 1990-1994
  • 1970-1974  (29)
Year
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 109 (1970), S. 46-54 
    ISSN: 1432-0878
    Keywords: Ovary ; Mouse ; Interstitial cells ; Endocrine tissue ; Innervation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Die Innervation der interstitiellen Drüse im Ovar der Maus wurde elektronenmikroskopisch untersucht. Die adrenergen Nerven wurden mit Hilfe der Falckschen Methode dargestellt. 1. Fluoreszenzmikroskopie: grün fluoreszierende Varikositäten findet man im Stroma ovarii vor allem in der näheren Umgebung von Gefäßen. Nur selten sind Nervenfasern in Komplexen von interstitiellen Zellen (IZ) zu erkennen. 2. Elektronenmikroskopie: Terminale Nervenfasern mit bekannter Innenstruktur erreichen die IZ von allen Seiten und können unabhängig von den Gefäßen verlaufen. Viele Axone durchsetzen die Basalmembran und treten in enge Beziehung zu interstitiellen Zellen. Dabei bilden sie teilweise kolbenförmige Anschwellungen nach Art von Synapsen, die tief in das Cytoplasma der innervierten Zellen eingebettet sein können. Der synaptische Spalt ist 200 Å breit. Spezialisierte prä- und postsynaptische Membranen kommen nicht vor. Die Bedeutung der Synapsen wird diskutiert.
    Notes: Summary Ultrastructural studies have been carried out on the innervation of the interstitial gland of the mouse ovary. In addition Falck's fluorescence method was applied. 1. Fluorescence microscopy: In the ovarian stroma green fluorescent nerve fibers are frequently to be found in the surroundings of large and small vessels. Seldom small fibers invade blocks of interstitial cells; however, their final ramification is not discernible. 2. Electron microscopy: Terminal fibers of the autonomic nervous system reach the cells of the interstitial gland from all sides. They may be independent from the course of the vessels. Many axons penetrate the basal membrane and come into close contact with interstitial cells, partly by forming large swellings (boutons), which may be deeply embedded into the cytoplasm of the innervated cell. The synaptic cleft is about 200 Å wide. Specialized pre- and postsynaptic membranes have not been found. The innervated cells show no peculiarities. The possible function of the synapses is discussed.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 109 (1970), S. 212-226 
    ISSN: 1432-0878
    Keywords: Ovary ; Interstitial cells ; Thecal gland ; Innervation ; Domestic fowl
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The fine structure of the nerves of the ovarian stroma of the domestic fowl is described for the first time. In the fowl, the nerves are concentrated upon blood vessels, smoth muscles and mainly, the thecal gland with the steroid-producing cells. Myelinated as well as unmyelinated nerve fibers were observed. Numerous axon terminals representing adrenergic and also presumptive cholinergic nerve fibers are regularly seen in membranous contact with steroid-producing cells. In these axon terminals microvesicles are oriented towards the steroid-producing cells indicating a specialization of the surface from axon-to-cell contact. Evidence has been presented that there is a membranous neuro-humoral contact between the peripheral autonomie nervous system and the steroid-producing cells in the ovary. The present investigation has demonstrated that there is morphologic evidence for a nervous control of steroid-producing cells. The physiological importance of this neuro-humoral contact is discussed.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 3
    ISSN: 1432-0878
    Keywords: Pituicytes ; Anuran ; Innervation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Pituicytes of Rana pipiens could be classified into two types, pale and dense, according to their relative densities of cytoplasm and the populations of free ribosomes and cell organelles. An intermediate type of pituicyte was also recognized. Lipid droplet such as are typical in the cytoplasm of mammalian pituicytes, are not in the cytoplasm of either types of frog pituicyte. Both types have long cytoplasmic processes which run among the nerve fibers, and some of them end at the pericapillary space. Nerve endings making synapse-like contacts with the cell bodies or the processes of the pituicyte are frequent. According to the structures and sizes of granules and vesicles in the nerve endings, these endings are classified into one of three types: 1) A, which appears to be a peptidergic neuronal ending containing dense granules 1,200–2,000 Å in diameter and small clear vesicles 300–400 Å in diameter; 2) B, which appear to be monoaminergic endings containing cored vesicles 600–1,000 Å in diameter and small clear vesicles 300–500 Å in diameter; 3) C, which appear to be cholinergic endings containing only small clear vesicles. Type C endings are relatively rare. In the synaptic area the axonal membranes appose those of the pituicytes across a gap of about 200 Å and numerous “presynaptic” vesicles are clustered or accumulated near the presynaptic membranes.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 111 (1970), S. 316-345 
    ISSN: 1432-0878
    Keywords: Electron microscopy ; Histophysiology of median eminence ; Avian neurohypophysis ; Neurosecretion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Résumé Les effets de l'adénohypophysectomie et de diverses sollicitations de l'axe hypothalamo-hypophysio-corticosurrénalien sur l'ultrastructure de l'Eminence Médiane (E.M.) ont été étudiés chez le Pigeon. 1. Chez le Pigeon entier, l'Eminence Médiane Caudale (E.M.C.) se distingue de l'Eminence Médiane Rostrale (E.M.R.) essentiellement par l'absence dans les deux couches les plus externes (couches palissadique et superficielle) de l'E.M.C. de granules de gros calibres (1600 à 1900 Å), la rareté de granules de diamètre moyen (1200–1400 Å) et la prédominance de petites vésicules à cœur dense de 600–800 Å. 2. La préhypophysectomie entraine: a) dans l'E.M.R. la quasi disparition de granulations dans les deux couches externes; b) dans l'E.M.C. la ≪vidange≫ de nombreux axones, mais un enrichissement relatif, parmi les granulations restantes, des granulations de gros calibre (1600–1900 Å) aux dépens des granules de plus petit calibre. 3. Un shock insulinique entraine des modifications du même ordre: a) déplétion des granules denses, limitée dans ce cas à la portion la plus antérieure des deux couches externes de l'E.M.R.; b) enrichissement relatif des granulations de moyen (1200–1400 Å) et de gros (1600–1900 Å) calibre dans l'E.M.C. avec, en plus dans l'E.M.C., un enrichissement en vésicules de type synaptique. 4. Un traitement à la métopirone produit un accroissement du nombre des granulations de moyen (1200–1400 Å) calibre dans les couches externes de l'E.M.R. et de l'E.M.C., et un enrichissement important de l'E.M.C. en vésicules de type synaptique. 5. Le traitement à la prednisolone conduit à un enrichissement très marqué des couches externes de l'E.M.R. en grains de 1200–1400 Å, et à un enrichissement des couches externes de l'E.M.C. en granulations de 1000 Å. Ces résultats sont discutés dans la perspective des régulations hypothalamo-corticotropes, particulièrement en ce qui concerne les granules de 1200–1400 Å.
    Notes: Summary The effects of adenohypophysectomy, and of several experimental interventions on the hypothalamo-pituitary-adrenal cortical axis have been studied in relation to the fine structure of the median eminence in the pigeon. 1. In control animals, the following morphological features of the caudal median eminence (C.M.E.) distinguish it from the rostral median eminence (R.M.E.): a) the absence in both external layers of the C.M.E. of large (1,600–1,900 Å) electron-dense granules, b) the presence in the C.M.E. of a small number of medium-size (1,200–1,400 Å) granules, and c) the predominance in the C.M.E. of small (600–800 Å) dense-core vesicles. 2. Adenohypophysectomy leads to: a) almost complete disappearance of electron-dense granules in both external layers of the R.M.E., and b) “emptying” of numerous axons and a relative increase in the number of large (1,600–1,900 Å) granules in the C.M.E. 3. Insulin shock produces modifications similar to those of adenohypophysectomy. The depletion of electron-dense granules from the axons is, however, restricted to the most anterior part of the R.M.E., and, in the C.M.E., the relative increase in the number of larger granules affects the 1,200–1,400 Å and the 1,600–1,900 Å size granules. 4. Metopirone enhances the number of medium-size (1,200–1,400 Å) granules in the external layers of both the R.M.E. and the C.M.E. and causes a significant increase in the number of synaptic-like vesicles in the C.M.E. 5. Prednisolone treatment leads to a marked enrichment of the external layers of the R.M.E. with 1,200–1,400 Å granules, and of the external layers of the C.M.E. with 1,000 Å granules. These results have been discussed with special reference to the hypothalamic control of the adrenocorticotropic function, especially reviewing the role of the 1,200–1,400 Å granules.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 106 (1970), S. 451-472 
    ISSN: 1432-0878
    Keywords: Hemocytopoiesis ; Insects ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Mikroskopische Beobachtungen an normalen „phagozytären Geweben“ (im Sinne der älteren Autoren) entlang des dorsalen Diaphragmas der beiden Orthopteren-Arten Gryllus bimaculatus und Locusta migratoria zeigen übereinstimmend, daß diese Bildungen eine hematopoietische Struktur haben. Bei beiden Arten entwickeln sich die blutbildenden Stammzellen aus einer großen Anzahl sog. Retikularzellen mesodermalen Ursprungs, die den Retikularzellen der blutbildenden Gewebe der Vertebrata sehr stark ähneln. Wie bei den Vertebrata differenzieren sich bei den Insekten die Blutzellen in sog. isogenen Zellgruppen von gleichem Typus und gleichem Entwicklungsstadium. Die starke phagozytäre Neigung der Retikularzellen erklärt, warum die blutbildenden Gewebe der Orthoptera von den älteren Autoren als phagozytäre Organe angesprochen wurden. Die hämatopoietische Differenzierung der Retikularzellen in reife Blutzellen (Haemozyten) findet entweder in einem lockeren Gewebe entlang des dorsalen Blutgefäßes, wie bei Locusta, statt, oder im inneren mehrerer, an das Herz gebundener, hoch organisierter blutbildender Organe, wie bei Gryllus, die noch stärker an die klassischen Strukturen der Vertebrata erinnern. Wir beschreiben im einzelnen beide Strukturtypen, insbesondere bei Gryllus die Einteilung der Organe in einen Cortex, in dem sich die Blutzellen bilden, und eine Medulla, in welcher sich die reifen Haemozyten ansammeln können. Nach starken Blutverlusten zeigen besonders die blutbildenden Gewebe von Gryllus eine dramatische Stimulierung der Hämatopoiese an; die Punktion der hämatopoietischen Organe kann also ebenfalls experimentell nachgewiesen werden.
    Notes: Summary Microscopic observations of the normal “phagocytic tissues” (in the sense of the classic authors) of the dorsal diaphragm in the two Orthopterans Gryllus bimaculatus and Locusta migratoria unequivocally demonstrate the hematopoietic nature of these cellular accumulations. In the two species, the hematopoietic elements develop from a large number of so-called reticular cells of mesodermic origin, which resemble closely the reticular cells of the hematopoietic organs of Vertebrates. As it is the case in Vertebrates, the differentiation of the hematopoietic elements into mature blood cells occurs in the two Orthopterans also in isogenic cell islets. The phagocytic activity of the reticular cells explains the fact that these organs were classically considered in the Orthopterans as simple phagocytic organs. The hematopoietic differentiation of the reticular cells can occur either in a poorly organized, loose tissue located along the dorsal vessel, as is the case in Locusta, or in a group of highly organized hematopoietic organs, as in Gryllus, which resemble far more the classical hematopoietic structures of Vertebrates. We give a detailed description of both types of organization, especially of the subdivision in Gryllus, of the hematopoietic organs into a cortex, where the haemocytes differentiate, and a medulla, where they can accumulate. After severe hemorrhages, the hematopoietic organs of Gryllus show all the features of a dramatic stimulation of hematopoiesis; their function can thus be experimentally demonstrated.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 106 (1970), S. 523-538 
    ISSN: 1432-0878
    Keywords: Ependyma ; Intracytoplasmic lipid bodies ; Histochemistry ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Bei erwachsenen Feuersalamandern und bei Salamanderlarven konnten in Ependymzellen sowie in subependymären Zellelementen des Gehirns und Rückenmarks zahlreiche rundliche intrazytoplasmatische Körper von starker Osmiophilie beobachtet werden, deren Durchmesser bis zu 12 μ betrug. Vereinzelt fanden sich diese voluminösen Gebilde auch im Cytoplasma von Satellitenzellen der Hirnnerven- und Spinalganglien. Das histochemische Verhalten und das Ultrastrukturbild der intrazytoplasmatischen Körper sprechen dafür, daß sie hauptsächlich aus Lipiden bestehen. Ihre funktionelle Bedeutung konnte bisher nicht eindeutig geklärt werden.
    Notes: Summary The ependymal and subependymal cells of the ventricular system and the central canal in adult und larval salamanders contain numerous unusually large intracytoplasmic osmiophilic spherical bodies with a diameter of up to 12 μ. Sporadically the bodies are found within satellite cells of peripheral ganglia. Histochemical and ultrastructural examination suggests that the bodies consist mainly of lipids. Their functional significance is unknown.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 108 (1970), S. 446-456 
    ISSN: 1432-0878
    Keywords: Loligo vulgaris ; Innervation ; Dense core vesicles ; Helicoidal muscle ; Filaments
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Die Innervation der braunen, roten und gelben Chromatophoren von Loligo vulgaris ist quantitativ und qualitativ verschieden. In den begleitenden Nervenbündeln finden sich stets Axone mit hellen (etwa 300 Å Ø), gelegentlich auch solche mit „dense-core“-Vesikeln (etwa 600 Å Ø). Die Myofilamente der kontraktilen Rinde sind gegeneinander versetzt und um die Längsachse spiralig gewunden. Im axialen Sarkoplasma treten gebündelte, in Längsrichtung zur Muskelzelle orientierte Filamente auf (jedes etwa 70 Å Ø), die möglicherweise eine Funktion bei der tonischen Kontraktion erfüllen.
    Notes: Summary The innervation of the brown, red and yellow chromatophore muscle cells in Loligo vulgaris shows quantitative and qualitative differences. The nerve bundles regularly contain axons with electronlucent vesicles of about 300 Å diameter, and occasionally axons with dense core vesicles of about 600 Å diameter. The myofibrils of the contractile cortex show a staggered arrangement and are wound in a spiral with respect to the axis of the muscle cell. In the axial sarcoplasm there is additionally a bundle of thin filaments of about 70 Å thickness. The bundle is orientated in parallel with the longitudinal axis of the muscle cell. Its function may be to maintain tonic contractions.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 109 (1970), S. 384-397 
    ISSN: 1432-0878
    Keywords: Heart muscle cells ; Electron microscopy ; Quantitative ; Asphyxia ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Normale und hypoxische Herzmuskelzellen aus der Wand des linken Ventrikels der Ratte wurden quantitativ-morphologisch anhand von elektronenmikroskopischen Längsschnitten nach Perfusionsfixierung untersucht. In normalen Zellen waren alle Myofibrillen relaxiert, die mittlere Sarcomerlänge betrug 2,2 μm. Die Schnittfläche wurde zu 55% von Myofibrillen, zu 27% von Mitochondrien und zu 18% von Grundplasma und Reticulum eingenommen. Die zwischen den Myofibrillen liegenden Mitochondrien waren längsoval und im Mittel 2,3mal so lang wie breit. Es bestand kein Unterschied zwischen subendokardial und subepikardial gelegenen Zellen. 10 min nach Erstickung der Tiere waren in den sonst unauffälligen Muskelzellen die Glycogengranula vermindert. Nach 20 min führte die Hypoxie zu einer Zunahme der relativen Schnittfläche der Mitochondrien um etwa 16% und zu einer beginnenden Kontraktur der Myofibrillen (Sarcomerlänge 2,0 μm). 20 min Hypoxie in Hypothermie (25–30°C intrathorakal) veränderte die normale Zellstruktur dagegen kaum. Wenn die Herzen während der 20 min dauernden Hypoxie in Normothermie mit einer procainhaltigen sauerstoff- und glucosefreien Blutersatzlösung durchspült wurden, waren die Myofibrillen relaxiert, die Schwellung der Mitochondrien dagegen wurde nicht reduziert. 30 min nach Erstickung wurde die Kontraktur stärker (Sarcomerlänge 1,7 μm). Nach 60 min bildeten sich Superkontraktionsknoten, einzelne Myofibrillen waren in Höhe der I-Bänder unterbrochen. Die Cristae der Mitochondrien wichen auseinander, die Schnittfläche der Mitochondrien hatte um 27% zugenommen. Während in Normotherapie eine Asphyxie des Tieres bereits nach 10 min die Herzmuskelzellen funktionell schwer schädigt, ist die Schädigung morphologisch erst nach 20 min eindeutig. Das bedeutet, daß für die elektronenmikroskopische Präparation eine Hypoxie von unter 10 min bedeutungslos ist. Hinsichtlich der morphologischen Manifestationszeit für die Unterbrechung der Sauerstoffversorgung stimmen unsere Befunde an Herzmuskelzellen gut mit vergleichbaren Angaben an Leberzellen überein.
    Notes: Summary In heart muscle cells of the left ventricle of rats the distribution of cell organelles and their reaction to hypoxia were investigated by electron microscopy. In normal hearts fixed by perfusion with aldehydes, the mean sarcomere length was 2.2 μm. 27% of the longitudinal sectional area was occupied by mitochondria, 55% by myofibrils and 18% by sarcoplasmic reticulum and ground plasm. The mitochondria situated in rows between the fibrils were oval and measured 2.3 times more in length than in width. There was no difference between cells from subendocardial and subepicardial regions. 10 min hypoxia (complete occlusion of the trachea) did not affect the appearance of muscle cells but diminished the number of glycogen granules. After 20 minutes the area occupied by mitochondria was increased by 16%, the mitochondria between the myofibrils were more spherical and only 1.5 times longer than wide. The sarcomeres shortened to 2.0 μm. With hypothermia (25–30°C) hypoxia of 20 minutes duration did not affect the cell structure. Perfusion of the heart by a saline solution, which contained procaine but neither oxygen nor glucose, for 20 minutes prevented shortening of the sarcomeres but not swelling of the mitochondria. 30 minutes after occlusion of the trachea the myofibrils shortened to a sarcomere length of 1.7 μm. After 60 minutes irregularly and excessively contracted myofibrils appeared and some sarcomeres were interrupted at the level of the I-bands. In some of the swollen mitochondria the cristae were widely separated. The increase of the area occupied by mitochondria was 27%. Asphyxia affects heart muscle cells severely with respect to function within 10 min, but morphologically it takes 20 min before a definite effect can be noticed. As to the time after which lack of oxygen is manifested morphologically, our results are consistent with findings in liver cells.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 109 (1970), S. 431-449 
    ISSN: 1432-0878
    Keywords: Contractile ring ; Cytokinesis ; Cell division ; Cytochalasin B ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Techniques of individual cell selection and precise ultramicrotomy have been employed to demonstrate that the contractile ring of cleaving HeLa cells is a transitory cytoplasmic organelle of distinctive fine structure and location. The contractile ring is an uninterrupted annulus encircling the equator of dividing cells exactly where the cleavage furrow forms. It is about 10 microns wide, up to 0.2 microns in thickness, and is composed exclusively of circumferentially aligned thin filaments 40–70 Å in diameter. Contractile ring filaments appear to be associated with the overlying plasma membrane. Controlled experiments with a mold metabolite (cytochalasin B) reveals that within a few minutes the drug abolishes the ability of HeLa cells to undergo cytokinesis. Cytochalasin B seems to decompose the contractile ring. It has no other clearly identifiable effects on other cell structures, notably the mitotic apparatus. Cytochalasin B is the only drug known which selectively inhibits cytokinesis in animal cells. In conclusion, the contractile ring is the most likely organelle responsible for cytokinesis in HeLa cells. Similar organelles probably occur in all cleaving animal cells. Successful cleavage depends upon the structural and functional integrity of the contractile ring.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 109 (1970), S. 465-480 
    ISSN: 1432-0878
    Keywords: Cerebral artery ; Innervation ; Degeneration of sympathetic nerves ; Synaptic vesicles
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The ultrastructure of the innervation of the anterior cerebral artery of the rat was studied in control animals and in animals after superior cervical ganglionectomy. Fluorescence histochemistry shows a periarterial network of intensely fluorescent fibers which are divided into two groups, adventitial and periadventitial. The fluorescence begins to decrease 26 hours after, and completely disappears about 32 hours after, ganglionectomy. Fine structural changes are first observed 18 hours after ganglionectomy, when the axoplasm of degenerating axons becomes electron dense. This density gradually increases up to about 32 hours. By 32 hours most axons with disintegrating axolemmas become inclusion bodies of the Schwann cells. At this stage, synaptic vesicles can still be distinguished as less dense areas, but the membrane structures of synaptic vesicles and mitochondria are difficult to recognize. The degenerating axons are gradually absorbed and by 38 hours dense, residual bodies are observed in the Schwann cells. Generally speaking, the degeneration occurs first in the adventitial fibers and then in the periadventitial fibers. The transient appearance of small, granular vesicles is noticed in axon terminals about 18 hours after denervation, although very few small, granular vesicles are seen in control tissue or at later stages of degeneration.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 11
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 110 (1970), S. 153-165 
    ISSN: 1432-0878
    Keywords: Reptiles ; Skin ; Keratin ; Electron microscopy ; Evolution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The different patterns of keratin formation that have evolved in the class Reptilia are all variations of a common process. In Squamata (snakes and lizards), a sequence of layers composed of α or β keratin is formed periodically, after which the old epidermal generation is shed. In Chelonia (turtles and tortoises), the epidermis of the shell is composed of only β keratin, whereas the skin of the neck and leg is composed exclusively of α keratin. Molting in toto does not occur and shedding is a continuous process comparable to that in avian and mammalian epidermis. In Crocodilia (crocodiles, caimans, alligators) there is only a single layer of cornified cells, but the composition of the layer varies in different parts of the scale. The hinge regions have many of the morphological characteristics of α and β keratin whereas the center resembles β keratin. The living cells beneath contain accumulations of keratohyalin. There are four ultrastructural characteristics of a keratinized α layer: 1) cellular outlines remain distinct, 2) a thickened plasma membrane forms during keratinization, 3) 80 Å filaments embedded in an amorphous matrix can be seen, and 4) PAS-positive material accumulates in extracellular spaces between the desmosomes. The β layer exhibits none of these features. Instead the cells more or less (depending on species) coalesce into a compact layer which becomes attenuated in the hinge regions. A 30 Å filament pattern can be seen. The mesos layer of squamates resembles the hinge region of crocodilians, exhibiting a combination of the characteristics of both α and β keratin.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 12
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 106 (1970), S. 309-321 
    ISSN: 1432-0878
    Keywords: Red nucleus ; Substantia nigra ; Neuroglia ; Pericytes ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The morphology of perivascular and perineuronal cells in the substantia nigra and red nucleus was studied in Nissl, silver carbonate, and electron microscopic preparations. In light microscopic preparations of the red nucleus and substantia nigra oligodendrocytes and astrocytes are located adjacent to blood vessels and nerve cells. Pericytes are also found adjacent to blood vessels. Scattered perineuronal oligodendrocytes and astrocytes are present in the magnocellular portion of the red nucleus and in the substantia nigra, whereas a distinguishing morphological feature of the parvocellular portion of the red nucleus is the clustering of perineuronal oligodendrocytes around a single neuron. In the present electron micrographs of the red nucleus and substantia nigra oligodendrocytes are separated from the vascular basement membrane (basal lamina) by astrocyte processes and therefore are not truly perivascular. Pericytes are easily identified by the basement membrane which encompasses their cell bodies and processes. Characteristic of the neuropil in the red nucleus are astrocytic processes that approximate dendrites. In contrast, astrocytic processes in the substantia nigra rarely contact dendrites which are covered by a mosaic of synaptic endings. A “third type of neuroglial element” is also present in the neuropil of the substantia nigra and the red nucleus.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 13
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 108 (1970), S. 324-338 
    ISSN: 1432-0878
    Keywords: Human myocardium ; Innervation ; Nerve endings ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Electron microscope studies of axons, distributing singly or in small bundles in the human ventricular and atrial myocardium, indicate a few per-cent of the axon profiles to be significantly large in diameter (1.5–3.0 μ). They are characteristically packed with a profuse number of mitochondria along with large granular vesicles, glycogen rosettes, lysosomic bodies; and some of them terminate on a “specific terminal cell” (Knoche and Schmitt). These mitochondria-rich, large axons are assumed to be terminal portions of the cardiac afferents. About half of the axons encountered in the ventricle and 2/3 in the atrium are non-vesiculated, usually less than 0.5 μ. in diameter. The varicosities containing numerous vesicles are mostly 0.5–1.5 μ in diameter and are assumed to be terminal portions of the cardiac efferents. The ratio between the number of axon profiles containing small granular vesicles and that of axon profiles containing agranular vesicles without small granular vesicles is 2∶1 in the ventricular myocardium and 1∶1.7 in the atrial myocardium.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 14
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 109 (1970), S. 33-45 
    ISSN: 1432-0878
    Keywords: Testis ; Interstitial cells ; Reptiles ; Hormone Production ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Résumé L'ultrastructure des cellules interstitielles du testicule de Lacerta vivipara a été étudiée entre le printemps et l'automne pendant deux années. Le retioulum endoplasmique lisse, et les mitochondries à crêtes tabulaires sont les organites les plus remarquables comme dans les autres cellules productrices de stéroïdes, mais les liposomes et l'appareil de Golgi sont bien représentés aussi. Les variations ultrastructurales les plus significatives apparaissent entre le printemps et le début de l'été. Au printemps, alors que les caractères sexuels secondaires sont hypertrophiés, un système remarquable de vésicules et de vacuoles se développe à partir du reticulum et probablement aussi du Golgi. Au début de l'été, lorsque les caractères sexuels secondaires sont atrophiés, les vacuoles sont moins nombreuses et le reticulum forme un réseau dense de tubules typiques, souvent étroitement associés aux liposomes; les crêtes mitochondriales sont gonflées. Ces images sont discutées en fonction de l'activité saisonnière d'élaboration d'hormones. L'hypertrophie des systèmes membranaires au printemps correspond probablement à la production ou (et) à l'excrétion des hormones androgènes. Au début de l'été, la cellule n'élabore pas d'androgènes, mais n'est peut-être pas complètement inactive: elle pourrait stocker des précurseurs hormonaux.
    Notes: Summary Interstitial cells of the testis of Lacerta vivipara have been studied electronmicroscopically in animals obtained between spring and autumn. Smooth endoplasmic reticulum and mitochondria with tubular cristae are the most prominent organels, lipid droplets and Golgi apparatus being also well developed. The most significant ultrastructural changes occur between spring and the beginning of summer. In spring, during the hypertrophy of secondary sexual characters, a conspicuous system of vesicles and vacuoles originates from the smooth endoplasmic reticulum and probably also from the Golgi apparatus. At the beginning of summer, when secondary sexual characters are atrophied, vacuoles are less prominent and the smooth endoplasmic reticulum consists of a dense network of typical tubules, often closely associated with the lipid droplets; the cristae of the mitochondria are swollen. These ultrastructural findings are discussed in relation to the production of hormones. The hypertrophy of membrane systems in spring corresponds presumably to production or (and) release of androgen hormones. In the beginning of summer the cell does not produce androgens, but probably is not completely inactive: it may store precursors of hormones.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 15
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 109 (1970), S. 495-516 
    ISSN: 1432-0878
    Keywords: Hilus cells ; Ovary ; Pig ; Structure ; Innervation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Elektronenmikroskopisch konnte bestätigt werden, daß es sich bei den Hiluszwischenzellen (Hz) im Ovar des Schweins um Leydig-Zellen handelt. Die Zellen verfügen über ein stark ausgeprägtes glattes endoplasmatisches Reticulum, das teils als tubuläres Netzwerk, teils als zwiebelschalenförmig angeordnete Häufung dicht gepackter, gefensterter Cisternen vorkommt. Diese Cisternen können in extremen Fällen die Hälfte eines Zellquerschnittes ausfüllen und kondensieren sich häufig um Lipidtropfen und Pigmentgranula. Die Mitochondrien besitzen Cristae, Tubuli und Sacculi sowie eine Matrix unterschiedlicher Elektronendichte. Die Golgi-Stapel sind schwach entwickelt. Die Zellen verfügen über Centriolen, und es gibt Hinweise, daß sie sich amitotisch teilen. Im Cytoplasma finden sich bis zu 5 μm große Lipidtropfen. Die in ihnen vorhandenen Pigmentgranula sind außerordentlich polymorph, meist von einer Membran umgeben und entstammen möglicherweise Lipidtropfen und Mitochondrien. Einzelne Axone des Hilusnerven können die Basalmembran durchbrechen und mit den Hz in engsten Kontakt treten. Typische Synapsen wurden nicht beobachtet. Die Befunde werden auf dem Hintergrund von Ergebnissen der Lichtmikroskopie, der Biochemie und Pathologie diskutiert und mit den an Leydig-Zellen gewonnenen verglichen. Verschiedene Zellbilder werden als Stadien unterschiedlicher Reife interpretiert.
    Notes: Summary The hilus cells in the ovary of the pig have been investigated with the electron microscope. These elements are identical with Leydig cells. The hilus cells contain an abundant agranular endoplasmic reticulum, which is either organized as a tubular network or as an onion like system of closely packed flattened cisternae sometimes filling up half of a cell and often being concentrated around lipid droplets and pigment granules. The mitochondria have cristae, tubuli and sacculi and a matrix of variable electron density. The golgi cisternae are poorly developed. The cells have centrioles, and there are hints of an amitotic cell division. The cytoplasm of the hilus cells contains lipid droplets, which have a diameter up to 5 μm. The pigment granules are extremely polymorphic, nearly always surrounded by a single membrane. They possibly may be derived from lipid droplets and mitochondria. Single axons of the hilus nerve occasionally penetrate the basement membrane and come into close contact with the hilus cells. Typical synapses were not observed. The results are discussed on the background of light microscopical, biochemical and pathological findings and compared with those obtained on Leydig cells of different species. Various cell images are interpreted to represent progressive stages of maturation.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 16
    ISSN: 1432-0878
    Keywords: Neurosecretion ; Gastropoda ; Fluorescence ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Durch Vergleich fluoreszenz- und elektronenmikroskopischer Untersuchungen am zentralen Nervensystem von Planorbarius corneus L. wird nachgewiesen, daß in den Schlundringganglien Neurosekretzellen vorkommen (Nachweis mit Pseudoisocyaninchlorid), die mit Nervenzellen nicht identisch sind, die durch ihren hohen Gehalt an biogenen Aminen auffallen (Nachweis durch die Methode von Falck und Owman, 1965). Es können daher im Schlundring von Planorbarius corneus peptiderge und aminerge Neurosekretzellen unterschieden werden. Die PSC-positiven Neurosekretzellen enthalten elektronendichte Elementargrana und die aminergen Neurone „dense-core“ Vesikel. Der Nachweis biogener Amine in einigen Nervenzellen von Planorbarius corneus spicht für deren chemische Identität mit Transmittersubstanzen, ihre hohe Konzentration aber für eine Abgabe in die Körperflüssigkeit.
    Notes: Summary The neurosecretory system of the snail Planorbarius corneus has been investigated by fluorescence and electron microscopy. With the fluorochrome Pseudoisocyanin the established neurosecretory system in the cerebral ganglia and single neurosecretory cells in the other ganglia show an intensive yellow fluorescence. Electron micrographs reveal the presence of electron dense granules (elementary granules) in the pericarya and the axons of neurones which have the same localisation in the ganglia as the pseudoisocyanin-positive cells. The fluorescence technique for biogenic amines produces yellow and green fluorescence within neurons and in the neuropil and nerves. The fluorescence obtained in determinable areas and neurones correlates well with the electron microscopic location of “dense-core” vesicles within the pericarya and axons of cells in even the same areas. It is discussed, that in this animal both types of cells are so-called “neurosecretory cells”, because the high content of elementary granules in the “peptidergic neurosecretory cells” and of “dense-core” vesicles in the “aminergic neurosecretory cells” is an indication for secretion of these products in neurohaemal areas (circulatory channels).
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 17
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 110 (1970), S. 321-335 
    ISSN: 1432-0878
    Keywords: Thymus ; Lymphocyte ; Analysis ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Thymic small lymphocytes of dd-mice were qualitatively and quantitatively studied by electron microscopy. Differences in fine structure were revealed between cortical and medullary small lymphocytes. Cortical small lymphocytes are rounded in cell outline with a round nucleus. The cytoplasm surrounding the nucleus as a narrow rim is scanty and appears relatively dense due to an abundance of free ribosomes. The cell organelles are not well developed. On the other hand, medullary small lymphocytes are more irregular in shape with uneven cell membranes. Their nuclei are also more irregular in outline with frequent infoldings of the nuclear membrane. The cytoplasm is more abundant and paler with less numerous ribosomes. The cell organelles are better developed. Quantitative analysis was made of both cortical and medullary small lymphocytes by means of the point counting method. The nuclei of both cortical and medullary small lymphocytes are almost the same in size (a diameter of 4.9 μ). The cell sizes are different between cortical and medullary lymphocytes: cortical small lymphocytes with a diameter of 5.5 μ were smaller than medullary ones with a diameter of 6.4 μ. Cortical small lymphocytes are very sensitive to the destructive effects of hydrocortisone, whereas the medullary ones are resistant. Periarteriolar lymphoid sheath in the splenic white pulp, which is known to be a thymus-dependent area, contains small lymphocytes that were similar in cytological details to medullary small lymphocytes of the thymus. In the light of the recent knowledge about a recirculating long-lived small lymphocyte pool, it appears probable that medullary small lymphocytes represent a contribution to the pool and that small lymphocytes with a long life span can be cytologically identified by electron microscopy.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 18
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 110 (1970), S. 386-400 
    ISSN: 1432-0878
    Keywords: Autonomic nerves ; Perineurial sheath ; Termination ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Das Perineurium kleiner Mesenterial- und Trachealnerven besteht aus einer Lage platter, beiderseits von einer Basalmembran umschlossener Zellen. Im Bereich von Abzweigungen kleiner Nerven bildet das Perineurium ein gekammertes, stark kollagenfibrillenhaltiges Hüllensystem. Die Endigung der einschichtigen Perineuralhülle hat die Form einer in das Interstitium geöffneten Röhre. Im ansatznahen Drittel des Mesenteriums und in der Schleimhaut der Trachea wurden ausschließlich perineuriumfreie Nerven gefunden. Es liegt nahe, diese perineuriumfreien Bezirke zu funktionellen Endräumen zusammenzufassen. In perineuriumfreien Bezirken wird das gehäufte Auftreten von Nervenauftreibungen mit Vesikeln, die Transmittersubstanz enthalten, und Mitochondrion beobachtet. Die erhobenen Befunde werden mit den an dickem Perineurium gewonnenen Ergebnissen verglichen und diskutiert.
    Notes: Summary The perineurial sheath of small tracheal and mesenteric nerves consists of a layer of flat, basement membrane coated cells. Its ramifications form a complicated layered system, interspersed with collagen fibrils. The tube formed by the thin perineurial sheath ends openly, which affords communication between the respective interstitial spaces. The mesenteric nerves close to the duodenum as well as the nerves in the vicinity of the tracheal epithelium show no perineurial sheath. Peripheral nerves lacking a perineurial layer seem to be close to their terminations. Their fibers show varicosities containing transmitter vesicles and mitochondria with longitudinally orientated cristae. Our results are compared with those found in thick, multilayered perineurial sheaths and functional differences are discussed.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 19
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 111 (1970), S. 51-63 
    ISSN: 1432-0878
    Keywords: Purkinje cell ; Golgi apparatus ; Dendrites ; Differentiation ; Histochemistry ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The morphology of postnatal differentiation of the Golgi apparatus, the nucleus, the perikaryon, and the dendrites was studied in Purkinje cells of the rat cerebellum for 30 days after birth using histochemical, histological, and electron microscopic methods. The Golgi apparatus during differentiation undergoes morphological and positional changes. From the 1st to 7th postnatal day, the Golgi apparatus is found in a supranuclear position, and is connected with the axes of differentiating primary dendrites by beam-like processes. From days 8 to 11 this connection disappears, and most of the Golgi apparatus assumes a lateronuclear and infranuclear position. After the 11th or 12th day, the Golgi apparatus is found in perinuclear and peripheral cytoplasmic positions. The formation of granular endoplasmic reticulum occurs in the vicinity of the perinuclear Golgi apparatus. The differentiation of cell and nuclear forms requires approximately 20 days. The morphological changes of differentiation are discussed in relation to the participation of the Golgi apparatus in the differentiation of dendrites and in the formation of the granular endoplasmic reticulum.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 20
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 111 (1970), S. 149-159 
    ISSN: 1432-0878
    Keywords: Rabbit ovum ; Fertilization ; Pronucleus ; Cleavage ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Fertilized ova were flushed from the Fallopian tubes of New Zealand White rabbits at 15 to 30 hours after mating and examined with the electron microscope. Between 21 and 22 hours intact pronuclei with extensive interdigitation of apposing surfaces were found in most ova. In some, an appearance suggesting internuclear communication was observed. In other ova disrupted pronuclear membranes surrounded centrally placed chromosome aggregates. In still others an advanced cleavage furrow was already present. By 23 hours all ova were in the two-cell stage.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 21
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 111 (1970), S. 404-412 
    ISSN: 1432-0878
    Keywords: Heart ; Innervation ; SA-Node ; Adrenergic and non-adrenergic axons
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The rabbit SA-node was outlined electrophysiologically and its adrenergic and cholinergic innervation patterns were studied with the electron microscope. Differentiation between adrenergic and cholinergic terminals was achieved by fixation of the specimens in KMnO4 which produces dense-cored synaptic vesicles in adrenergic terminals, whereas synaptic vesicles in cholinergic terminals are empty. It was found that adrenergic and cholinergic nerve terminals often come in close apposition to each other, the distance between adjoining membranes being in the order of 250 Å. At times, faint membrane thickenings could be seen in these places. The available pharmacological, physiological and morphological evidence leaves little room for doubt that cholinergic terminal fibers can influence the adrenergic ones. From mainly morphological evidence it is also postulated that adrenergic terminals influence cholinergic terminals.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 22
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 111 (1970), S. 413-426 
    ISSN: 1432-0878
    Keywords: Median eminence ; Pars nervosa ; Innervation ; Amphibian
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Résumé L'éminence médiane et la pars nervosa de Rana esculenta diffèrent du point de vue de leur structure. L'éminence médiane se compose de 2 zones différentes: la zone externe placée près du lobe distal et la zone interne située sous l'épendyme. Dans la zone externe, on distingue, d'après la taille et la forme des grains de sécrétion, 5 types de terminaisons. 1. des terminaisons avec de fins granules sphériques denses de 800 à 1000 Å de diamètre; 2. des terminaisons avec des granules de 1000 à 1200 Å de diamètre; 3. des terminaisons avec des grains de forme irrégulière de diamètre supérieur aux précédents (1200 à 1600 Å); 4. des terminaisons avec de volumineux grains denses sphériques d'environ 1200 à 1800 Å de diamètre; 5. un petit nombre de terminaisons ne contenant que des vésicules. Les terminaisons des catégories 3 et 4 sont probablement du type neurosécrétoire. La zone interne contient de nombreuses fibres neurosécrétrices. Elles sont de 2 types, l'une avec de gros granules (1600–2400 Å), l'autre avec des granules moins volumineux (1300–2000 Å). Des fibres non neurosécrétrices ont également été observées. Dans la pars nervosa, on rencontre deux types principaux de fibres neurosécrétrices, l'une avec des grains denses de 1600 à 2400 Å de diamètre, l'autre avec des grains moins denses d'environ 1300 à 2000 Å de diamètre. Dans la zone externe bordant la pars intermedia des fibres aminergiques avec de fines granulations ont été observées.
    Notes: Summary The median eminence and the pars nervosa of Rana esculenta have a different structure. The median eminence has 2 different zones: the outer zone situated near the pars distalis and the inner zone under the ependyme. In the outer zone there are, according to the size and the shape of the granules, 5 types of nerve terminals. 1. Endings containing spherical fine dense granules of 800 to 1000 Å in diameter; 2. Endings with spherical granules from 1000 to 1200 Å in diameter; 3. Endings with granules of irregular shape which are bigger than the former (1200 to 1600 Å); 4. Endings with spherical dense granules of about 1200 to 1800 Å in diameter; 5. A few endings containing only clear vesicles. Type 3 and type 4 endings are probably neurosecretory. The inner zone contains numerous neurosecretory fibres. They are of two types: one with big granules (1600–2400 Å), the second with smaller granules (1300–2000 Å). Non-neurosecretory fibres have also been observed. The pars nervosa contains two principal types of neurosecretory fibres: one with dense granules of 1600 to 2400 Å in diameter, the other with lighter granules of about 1300 to 2000 Å. In the external zone lining the pars intermedia, aminergic fibres with fine granules have been observed.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 23
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 103 (1970), S. 382-397 
    ISSN: 1432-0878
    Keywords: Adrenal cortex ; Newborn rat ; Stereology ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The structure of the zona glomerulosa and the zona fasciculata of the newborn rat adrenal cortex has been studied by the light and the electron microscope. The ultrastructural features of the cells of both zones are described. By morphometric methods the relative volumes occupied by mitochondria and by lipid droplets have been evaluated. The “membrane profile concentration” (i.e. the average number of microns of smooth reticulum profiles per square micron of cytoplasm) has been also measured. The most significant ultrastructural differences between the cells of the two zones concern the mitochondria, the lipid droplets, and the smooth endoplasmic reticulum. The mitochondria show typical lamellar cristae in the elements of the zona glomerulosa, and vesicular cristae in the cells of the zona fasciculata. They occupy the same relative volume in both elements. The lipid droplets and the smooth reticulum show no qualitative differences in the two cell types, but they are found in a larger amount in the cells of the zona fasciculata. These ultrastructural findings are discussed in relation to the numerous biochemical data, suggesting that the cells of the zona glomerulosa are resting elements at birth, while the cells of the zona fasciculata are active steroid-secreting elements already in late gestation.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 24
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 103 (1970), S. 398-409 
    ISSN: 1432-0878
    Keywords: Adrenal cortex ; Newborn rat ; Electron microscopy ; Lysosomes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The structural changes of the zona juxtamedullaris of the rat adrenal cortex at birth, have been examined by the light and the electron microscope. In this zone clusters of medullary cells lying among the strands of cortical tissue were observed. In the inner portion of the zona juxtamedullaris two types of adrenocortical cells were found: light and very-dark cells. The latter are smaller than the light cells and are always in close connection with the medullary tissue. The ultrastructural features of the very-dark cells suggest that these elements are in degeneration. This finding supports the hypothesis that at birth there is a partial degeneration of the rat zona juxtamedullaris, i.e. the zone corresponding to the “fetal zone” of some mammalian species. It is proposed that in all mammalian species at birth there is a partial regression of the zona juxtamedullaris and that the regression of the “fetal zone” is only the quantitative increase of this phenomenon. This hypothesis is discussed in relation to numerous data demonstrating that there are enzymatic conditions in the rat during fetal life, which permit a discrete hypertrophy of the adrenal cortex.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 25
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 103 (1970), S. 341-350 
    ISSN: 1432-0878
    Keywords: Catecholamines ; Brain ; Insects ; Electron microscopy ; Cytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In the brain of the cockroach Periplaneta americana, the beta lobes of the corpora pedunculata respond with an intense positive reaction to a specific fluorescence histochemical method for catecholamines. The fluorescence reaction disappears completely after prolonged treatment of the cockroaches with reserpine. An ultrastructural examination of the beta lobes in formaldehyde-glutaraldehyde-osmium fixed preparations reveals the presence of two types of fibres: 1) Fibres and nerve endings containing small clear vesicles and sligthly larger vesicles with a semi-dense content. The appearance and size distribution of these vesicles ist not affected by treatment with reserpine. 2) Fibres containing larger and denser vesicles, but practically no clear vesicles. The size distribution of these dense vesicles is only slightly affected by treatment of the cockroaches with reserpine. If brain slices are incubated in a medium containing noradrenaline or α-methyl-noradrenaline and fixed in permanganate, small vesicles with electron-dense central cores show up, similar to those which have been described in vertebrate adrenergic nerve fibres (“small granular vesicles”). They are confined to one of the two types of fibres (a and b) visible in these preparations, namely to type b, whose correspondence with type 2 fibres of formaldehyde-glutaraldehyde-osmium fixed preparations is discussed.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 26
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 104 (1970), S. 345-357 
    ISSN: 1432-0878
    Keywords: Photoreceptor ; Lumbricus ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Die Feinstruktur der Photorezeptorzellen in der Epidermis, in kleineren Nervenästen und im Zerebralganglion von Lumbricus terrestris wurde untersucht. Das Vorhandensein eines zentralen, intrazellulären Lumens (Phaosom), das mit Mikrozotten gefüllt ist, erinnert in der Struktur der Photorezeptorzelle des Regenwurms an Lichtsinneszellen von Hirudo. Außer Mikrozotten findet man im Phaosom einige Zilien vom Typ 9×2+0; solche Zilien sind von den Mikrozotten strukturell unabhängig. Durch eine Aufzweigung des Phaosoms in kleinere Buchten, die tief in das umgebende Zytoplasma eindringen, erhält es ein labyrinthartiges Aussehen. Glatte Zisternen in Gruppen von 2 bis 5 wurden oft um das Phaosom im Zytoplasma beobachtet. Charakteristische Bestandteile der Zelle sind noch Vesikel und Vakuolen, die mit einer Substanz von wechselnder Elektronendichte gefüllt sind. Die Photorezeptorzellen werden von Gliazellen und Gliafortsätzen umgeben, die an vielen Stellen die Zelloberfläche tief einstülpen (Trophospongium).
    Notes: Summary Photoreceptor cells in the epidermis and nerve branches of the prostomium and in the cerebral ganglion of Lumbricus terrestris were investigated with the electron microscope. The photoreceptor cell is similar to the visual cell of Hirudo by having a central intracellular cavity (phaosome) filled with microvilli. Besides microvilli, several sensory cilia can also be found in the phaosome but they are structurally independent of the microvilli. A gradual branching of the phaosome cavity into smaller cavities makes its sectional profile extremely labyrinthic. Flattened smooth-surfaced cisternae in stacks of 2 to 5 are frequently observed around the phaosome. Characteristic constituents of the cytoplasm are vesicles and vacuoles filled with a substance of varying density. The photoreceptor cell is covered by glial cells or by their processes which at many places deeply invaginate the cell surface (trophospongium).
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 27
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 105 (1970), S. 176-187 
    ISSN: 1432-0878
    Keywords: Ependyma ; Salamandra maculosa ; Neuronal elements ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Zwischen den eigentlichen Ependymzellen des Zentralkanals adulter Feuersalamander kommen amphorenartig gestaltete Elemente vor, die sich aufgrund ihrer synaptischen Kontakte mit Axonendigungen als Neurone identifizieren lassen. Diese intraependymalen Nervenzellen weisen einen apikalen Fortsatz auf, der sich mit einer warzen- oder knotenförmigen Protrusion in das Lumen des Zentralkanals erstreckt. Die Protrusion ist gewöhnlich mit stereozilienartigen Ausläufern besetzt. Die funktionelle Bedeutung der beschriebenen neuronalen Elemente konnte bisher nicht geklärt werden.
    Notes: Summary The ependyma of the canalis centralis of adult salamanders was examined by electron microscopy. Between the ependymal cells occur amphora-like elements identifiable as neurons by their synaptic contacts with axon terminals. These intraependymal nerve cells exhibit an apical outgrowth extending into the lumen of the canalis centralis with a wart-like or knob-like protrusion. The latter usually bears extensions resembling stereocilia. The functional significance of the neuronal elements is still unknown.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 28
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 105 (1970), S. 538-542 
    ISSN: 1432-0878
    Keywords: Eye ; Iris ; Melanophores ; Innervation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The nerve supply to the iridic melanophores of the rat was studied with the electron microscope. The adrenergic and cholinergic terminals were identified with the aid of 5-hydroxydopamine, which produces dense-cored 400–800 Å synaptic vesicles in adrenergic axon varicosities, whereas the synaptic vesicles of cholinergic axons remain empty. It was found that both adrenergic and cholinergic terminal axons come in close apposition (200–250 Å) with the melanophores. The appositions have the same appearance as synapses in peripheral tissues. It seems likely that the murine iridic melanophores have a double innervation, although its functional significance is obscure.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 29
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 105 (1970), S. 515-525 
    ISSN: 1432-0878
    Keywords: Cuticle ; Rotifer ; Development ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Ultrastructural studies of developing Asplanchna brightwelli embryos support the following hypothetical scheme of cuticle formation. First the external hypodermal membrane invaginates, and deposition of a dense intracellular layer commences next to this membrane. Then the rough endoplasmic reticulum synthesizes fibrous protein which is transferred to the Golgi complex. Here polysaccharide is synthesized and added to the protein, and the resulting filamentous complex is enclosed in large irregularly shaped vesicles which bud off from the Golgi elements. Maturation of the filamentous material to condensed cuticle material occurs as the vesicles move to the invaginations. Each vesicle fuses with an invagination, thus forming a hypodermal bulb; then the cuticle material is discharged through the neck of the bulb to its extracellular location. After the bulbs are formed, new, smaller, spherical vesicles begin to bud off from the Golgi elements. They too contain the filamentous complex which is refined to condensed cuticle material as the vesicles near the bulb. These vesicles fuse with the hypodermal bulbs contributing the cuticle and membrane necessary for the growth of the hypodermis of the embryo and newborn animal. Ruthenium red staining has confirmed that the cuticle consists of glycoprotein.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...