ALBERT

Notes: SYNOPSIS. A new species of coccidium is described: Eimeria urosauris n. sp., in the gall bladder of the lizard Urosaurus graciosus Hollowell, from the Mojave Desert in California. The oocyst of E. urosauris is smooth, bilaminar, nearly cylindrical, with long borders only slightly convex, ends rounded and very nearly hemispherical. It is usually 32 × 20 μ, and its length/width ratio is 1.6. It contains 4 ellipsoid sporocysts, each 10.5 × 9 μ, for which 1/w is 1.17. Each sporocyst contains 2 tapered bent sporozoites with rounded ends, 11 μ long, 4 μ in diameter at the larger end, and 1.5 μ in diameter at the smaller end. Each sporocyst also contains a central granular sporocyst residuum 3.5 μ in diameter. The oocyst lacks a micropyle and oocyst residuum, and there is no Stieda body on the sporocyst. Sporulation time is 6–10 hr. Endogenous development, with reinfection by liberated sporozoites, occurs in the epithelial lining of the gall bladder. E. urosauris is compared to other morphologically similar lacertilian eimerias with which it might be confused.

Notes: SYNOPSIS. In rats infected with T. lewisi, 2 morphologically distinct forms of the trypanosome occur. By means of the immunodiffusion technic several antigenic differences between the 2 forms were observed. The time course in the antigenic and morphologic changes is somewhat different. The antigenic change from young to adult forms is gradual. It starts 12 days after infection and is complete in 14 days, 3 to 5 days after the morphologic appearance of the trypanosomes is that of adult forms. In addition, there appear to be 2 separate stages of young forms when they are analyzed for antigenic composition.

Notes: SYNOPSIS. Nucleic acid hybridisation involving DNA samples was used to study relationships between various protozoa. The most hybridisation or nucleic acid homology was always in the homologous reaction between 2 DNA samples from the same source. It was concluded that all the protozoa tested have nucleotide sequences or genes in common with Paramecium aurelia. The ciliates could be placed in a sequence of decreasing homology relative to Paramecium: Tetrahymena, Colpidium, Stentor, Didinium, Dileptus, Blepharisma. Actinosphaerium had fewer sequences than any of the ciliates and the flagellate Euglena had the fewest sequences in common. The bacterium Aerobacter had none. Similar relationships were inferred from competitive hybridisation experiments; these relationships were also in general mirrored by morphologic relationships and overall G + C base compositions which ranged from 46% for Euglena to 32% for P. aurelia. These experiments, it is hoped, will contribute to studies on origins of the metazoa.

Notes: SYNOPSIS. Nicotinamide adenine dinucleotide phosphate (NADP) and nicotinamide adenine dinucleotide (NAD) linked isocitrate dehydrogenase and NADP linked alcohol dehydrogenase have been detected in Euglena gracilis var. bacillaris.The NADP isocitrate dehydrogenase showed half-maximal activity at a concentration of 3 × 10−5 M DL-isocitrate, but did not follow simple Michaelis-Menten kinetics with respect to substrate concentration. The optimal NADP concentration was about 0.06 mM, and activity fell off sharply on either side of this optimum. Fresh preparations of the enzyme migrated as single bands in disc electrophoresis, but two enzymatically active bands were present after frozen storage.The NAD isocitrate dehydrogenase followed Michaelis-Menten kinetics with respect to substrate. In crude extracts, no requirement for adenosine monophosphate, adenosine diphosphate, or sulfhydryl compounds could be found.NADP alcohol dehydrogenase activity could be found with either ethanol or propanol as substrate. Low concentrations of coenzyme A were moderately inhibitory.In tris(hydroxymethyl) aminomethane buffer (tris buffer), Euglena extracts reduced NAD slowly in the absence of exogenous substrate. In the absence of tris, no such reduction occurred. A similar phenomenon was observed with NADP.

Notes: SYNOPSIS. Hepatocystis has been found extremely common in a number of species of Formosan mammals, notably the large leafnosed bat (Hipposideros armiger terasensis), the Formosan macaque (Macaca cyclopis), and various subspecies of the red-bellied tree squirrel (Callosciurus erythraeus). Less often infected were the long-winged bat (Miniopterus schreibersii) and the Formosan giant flying squirrel (Petaurista grandis). The species occurring in H. a. terasensis is believed to be new, and is named Hepatocystis hipposideri. It causes considerable enlargement (but not other changes) of the host cell, is often amoeboid, and usually has numerous fine pigment granules.

Notes: SYNOPSIS. Tracer technic has proved to be an excellent tool in the study of predator-prey relationships among the foraminifera. More than fifty axenic species of protists including diatoms, dinoflagellates, chlorophytes, chrysophytes, cyanophytes, bacteria and yeasts were tested as potential food for Allogromia sp (NF), A. laticollaris, Am. monia beccarii, Quinqueloculina spp, Rosalina floridana, Anomalina sp, Elphidium sp, Spiroloculina hyalina, Globigerina bulloides, and Globorotalia truncatulinoides. Although many types of potential food are present in the environment, foraminifera select only certain organisms. The yeasts, cyanophytes, dinoflagellates, chrysophytes and most bacteria tested were not eaten. Selected species of diatoms, chlorophytes and bacteria were eaten in large quantity. Three additional factors affect feeding: the “age” of the food organism, the “age” of the foraminifer or its position in the life cycle, and the concentration of the food. Feeding by foraminifera on most food is erratic below a concentration of 103 organisms and is approximately directly proportional to concentration within a range of 103-106 organisms per 10 ml experimental tube. A natural bloom of Protelphidium tisburyensis was analyzed. A high concentration of 6 species of diatoms characterized the community. A “bloom”-feeder hypothesis for foraminiferal nutrition is presented.

Notes: SYNOPSIS. The reorganization in resting cysts which produce a single emerging individual was followed in 5 species of Colpodidae forming a possible evolutionary series with increasing numbers of kineties. Stomatogenesis in Colpoda maupasi and C. inflata was studied for the first time; that in C. steinii, C. cucullus and Tillina magna was reinvestigated with new observations. In all, the oral primordium is double and originates from segments of certain somatic kineties, constant for each species. The larger mouthparts in the larger ciliates are produced from a correspondingly greater number of kineties. Morphogenetic and evolutionary implications of this series of observations are discussed.

Notes: SYNOPSIS. When large batches of Paramecium (more than 100,000 cells) are centrifuged in conventional centrifuges, extensive lysis occurs, typically lowering recovery to around 30%. An easily constructed centrifuge with a cylindrical plastic head operated at low accelerations permits centrifugation of large batches of Paramecium with about 90% recovery, even after exhaustive washing. Operation requires only a few minutes, and the system is easily cleaned. The inexpensive heads can be instantly interchanged, and cultures can even be centrifuged in their original low form glass culture vessels. This system, which should be applicable to any large fragile cells, permits timed experiments that are usually impossible because of the need for exhaustive washing or concentration from large initial volumes.

Notes: SYNOPSIS. Defined media for Leptomonas collosoma, L. mirabilis, and L. sp. from Dysdercus are described. In addition to factors required by Crithidia spp., including a source of Crithidia factor, all 3 organisms require glycine. Leptomonas collosoma and L. sp. from Dysdercus require choline. All trypanosomatids thus far studied require calcium pantothenate but L. collosoma has an especially large requirement. Leptomonas mirabilis but not L. sp. from Dysdercus can synthesize methionine from homocysteine thiolactone during growth. Homocysteine thiolactone is toxic to L. sp. from Dysdercus. Methionine plus vitamin B12 partly annul homocysteine thiolactone toxicity but only if glycine is also present.

Notes: SYNOPSIS. Glauconema trihymene n. g., n. sp., a marine hymenostome ciliate, is described from the Virginia coast. Morphologic studies were made on specimens treated with the Chatton-Lwoff silver impregnation technic and on animals observed with the phase microscope. Particular attention is given to the buccal ciliature and its importance to generic assignment in the order Hymenostomatida.

Notes: SYNOPSIS. Hematozoa were found in 89 (30.6%) of 291 cliff swallows (Petrochelidon pyrrhonota), captured for banding purposes. Hepatozoon was found in 73 (25%), Trypanosoma in 13 (4.5%), microfilariae in 9 (3%), and Haemoproteus and Leucocytozoon in 1 bird each. Multiple infections were found in only 7 of the swallows.

Notes: SYNOPSIS. Earlier experiments suggested that the species of Haemoproteus which infects English wood-pigeons (Columba p. palumbus) and is transmitted by the hippoboscid dipteran fly Ornithomya avicularia, was unable to infect Columba livia domestica, a host of H. columbae. This has now been confirmed by the injection of sporozoites into two pairs of pigeons, one of each species; in both experiments the C. palubus developed parasitemia while the C. livia did not. It has also been shown that this species of Haemoproteus can complete sporogony in a small proportion (2/73) of Pseudolynchla canariensis, the vector of H. columbae.

Notes: SYNOPSIS. Cilia devoid of their basal granules and isolated from Tetrahymena pyriformis strain W, by the method of Watson et al. (66) have been characterized in terms of physical, chemical and immunologic properties.The general chemical composition of cilia, based on mean values from these determinations is 66.3% protein, 24.3%“lipid,” 3.7 or 4.8% carbohydrate, and 0.4% nucleic acid. The amino acid composition of cilia includes hydroxyproline and is not significantly different from the amino acid composition of whole cells. Chloroformmethanol extracts of ciliary material contain free amino acids as well as neutral lipids and phospholipids. The lipid composition of cilia also is not significantly different from that of whole cells. Pentose, hexose and small but significant amounts of adenine nucleotide and ribonucleic acid are present.Cilia are a greater stimulus for the production of antibody than an equivalent amount of cellular antigen, and the ciliary antigen is strain specific.

Notes: SYNOPSIS. Macrophages were infected in vivo with the intracellular form of Leishmania donovani (LDs), harvested from the previously saline-stimulated peritoneal cavities of hamsters and explanted into Leighton tubes containing removable coverslips. Serum from either rabbit, chicken, human, calf, hamster or cotton rat blood was used as the 40% component of a Hanks' BSS60 serum40 medium used to maintain these Leighton tube cultures at 37 C. After varying lengths of time coverslips were removed from tubes, stained with Giemsa, and the parasites per infected macrophage, total number of hamster cells and total number of parasites on each coverslip were counted.Maerophages constituted more than 90% of the explanted cells on the coverslips. When cotton rat serum was used as a component of the medium, fibroblastic overgrowth of the coverslips followed. Some similarities and differences in the numbers of macrophages, fibroblasts and parasites were noted with regard to the serum used as part of the medium. Except for cotton rat serum, the serum component of the medium used apparently did not influence, to any great degree, the morphology of either the macrophages or parasites therein. Thus, vacuolarization and granularization of macrophages did not appear to be very distinctly correlated with the time of sampling or the type of serum in the medium used for maintenance nor could any morphologic variations of the LDs be ascribed to these factors.When cotton rat serum, but not any of the other sera, was the serum component of the medium, leptomonads were noted in the overlay fluid of the cultures after 6 days.Under these conditions of cell culture, fibroblasts could not be infected with LDs although macrophages on the same coverslip were heavily parasitized.

Notes: SYNOPSIS. Spirostomum ambiguum equilibrated with 45Ca in the external medium after about 14 days under normal conditions. As the external Ca concentration was increased, uptake was increased but only until the internal concentration was ∼ 60 mM. Changing the Ca/K ratio or increasing the K or Mg concentrations had no effect on uptake of the isotope; this was taken as an indication that most of the uptake reflected Ca binding as apatite. Increasing and decreasing the temperature increased and decreased Ca uptake, but Ca bound at higher temperatures remained in the animal at lower temperatures. Feeding caused a marked increase in uptake of isotope, probably due to the availability of a large number of binding sites on the products of digestion perhaps accompanied by increased permeability of the cell membrane. It is argued that most of the 45Ca taken up is incorporated into Ca phosphate deposits, although it is likely that there will be a certain amount of free or weakly bound Ca.

Notes: SYNOPSIS. Some neutral carbonyl compounds synthesized by Cryptomonas, ovata var. palustris were identified as methanal, ethanal, 2-propanone, pentanal, and heptanal by means of thin-layer chromatography, gas chromatography and infrared spectroscopy.

Notes: SYNOPSIS. Tetrahymena vorax is a small polymorphic holotrichous ciliate capable of transforming to macrostomes when microstomes are washed and suspended with prey in distilled water.Extrinsic factors having an effect on this transformation were examined; maximum yields of macrostomes (in excess of 90%) were obtained under the following conditions: populations of both prey, T. pyriformis, and potential predator, T. vorax microstomes, were grown on Loefer's medium for 48 hours prior to washing in distilled water. The density of the prey was adjusted to 300,000 cells/ml and the predator density to 2,000–3,000 cells/ml. Five ml of prey suspension and 5 ml of T. vorax microstome suspension were mixed together in a large petri dish because a high surface-volume ratio is important for high yields of macrostomes. The pH was adjusted to 6.0 and the petri dish was placed at 20 C for 12 hr. Macrostomes then appeared about 6 hr after addition of the prey.A dialyzable, heat stable substance released by the prey which can induce the microstome-macrostome transformation was isolated. This material was effective after being stored for weeks in the cold; its activity was not affected by the protein digesting enzymes pepsin or trypsin. This factor was called stomatin because its first visible effect in producing microstome-macrostome transformation appeared to be to incite reorganization of the oral structures.

Notes: SYNOPSIS. Plasmodium hegneri n. sp. is described from the European teal duck, Anas c. crecca, from Taiwan. The blood stages, so far the only ones seen, are distinguished mainly by the elongate character of the gametocytes, which closely resemble Haemoproteus (though the pigment tends to be finer and less abundant), and the failure of both asexual and sexual forms to displace the nucleus or otherwise alter the host cell. Merozoites average 13.4 ± 2.2 (range 10–19) per segmenter. Trophozoites often adhere to the host cell nucleus, and may have a large vacuole and a remarkable long, slender pseudopodium. The species so far has been seen only in the European teal, although blood films from 194 species and over 1200 birds have been examined.

Notes: SYNOPSIS. Potomacus pottsi n. g., n. sp., a brackish water hymenostome ciliate, is described from the Potomac River. Morphologic studies were made on specimens treated with the Chatton-Lwoff silver impregnation technic. Polymorphism, with the formation of large macrostome forms, was noted. Particular attention is given to the buccal ciliature and its importance to generic assignment in the order Hymenostomatida.

Notes: SYNOPSIS. Pseudocohnilembus marinus n. sp., a marine ciliate, is described from the Virginia coast. Morphologic studies were made on specimens treated by the Chatton-Lwoff silver impregnation technic. Particular attention is given to the infraciliature of the buccal apparatus and its importance to the systematics of the order Hymenostomatida. The buccal infraciliature of the new species differs from that of all other species of Pseudocohnilembus by the presence of a lateral bar of argentophilic granules at the posterior end of the inner membrane.

Notes: SYNOPSIS. When the structures involved in digestive events in T. pyriformis are examined at the electron microscope level, some information is added to that long known from light microscopy. The food trapping mechanism consists of the three membranelles, undulating membrane, oral ribs, and a “valve” apparently closing the opening to the cytopharynx. Both of the latter structures are supported by microtubules. Fibers extend internally from the cytopharynx and are closely associated with the food vacuole as it forms.Clear vacuoles resembling pinocytic vacuoles appear to arise from differentiated areas of the pellicle and plasma membrane. These vacuoles may fuse with primary lysosomes. Hydrolases are thus contributed to the pinocytic vacuoles which may then fuse with food vacuoles. When first formed food vacuoles contain no hydrolases but may acquire them directly, from primary lysosomes or from pinocytic vacuoles. Digestion proceeds to completion in the food vacuole, at which time soluble food products are released to the cytoplasm. Undigested materials are lost through the cytopyge. In stationary growth phase cells autophagic vacuoles form containing mitochondria and other cellular particulates. Such vacuoles probably contain hydrolases when formed and they may receive others by fusion with primary lysosomes.

Notes: SYNOPSIS. Heat shock and stationary-phase conditions both cause fusion of nucleoli. In both cases the process is reversed when the cell is returned to normal physiological growth conditions. Fusion of nucleoli during the cell cycle of logarithmically growing cells was not observed. Likewise, fusion of nucleoli was not observed when the Padilla and Cameron(8) method of synchronization was used. The macronuclei of cells synchronized by the 1 cold-shock per cycle method(8) more closely resembled macronuclei of log-phase cells than did the macronuclei of cells synchronized by the Scherbaum and Zeuthen(12) heat-shock method.

Notes: SYNOPSIS. The presence of anemia that often seems excessive for the amount of parasitemia in Plasmodium berghei infections had led to the suggestion that autoimmunity might be in part responsible for the anemia. In another erythrocytic infection, Anaplasma marginale of cattle, the association of erythrophagocytosis, autohemagglutination and anemia with infection has led to the suggestion that autoimmunization may occur in anaplasmosis. The possibility that similar findings might be present in P. berghei infections of rats has been investigated. Groups of rats infected with P. berghei were examined at 2–3 day intervals during the course of infection. Red blood cell counts, hematocrit values and percentages of parasitized erythrocytes were determined. The rats were bled at intervals and the sera tested for agglutinins for trypsinized rat erythrocytes. Other infected rats were killed, and their spleens and bone marrow were examined for evidence of erythrophagocytosis.Parasitemia reached a peak on the 9th day of infection and became subpatent by the 14th. The greatest depression in erythrocyte numbers occurred on the 11th day, and the counts remained below normal until the 23rd day. Phagocytized erythrocytes, predominantly uninfected, were found in the phagocytes of the spleen and bone marrow from the 5th through the 21st day. Agglutinins for trypsinized normal rat erythrocytes were present in the sera of the rats in titers as high as 1:64 from the 5th through 14th day of infection. Lower agglutinin titers (1:8) were found from time to time in sera of rats made anemic by repeated bleedings.It is not clear whether these agglutinins are responsible for erythrophagocytosis; however, the fact that predominantly uninfected erythrocytes were phagocytized suggests that the erythrocytes might have been opsonized by an autoantibody associated with the P. berghei infections.

Notes: SYNOPSIS. Entamoeba histolytica grown with H3-thymidine in CLG medium took up tritium into DNase-sensitive material in the nucleus and cytoplasm. The distribution of nuclear activity indicated that the entire nucleus, including the peripheral chromatin, may possess DNA; previous investigators reported DNA only in the endosome.The penicillin-inhibited bacterial associate (Bacteroides sp.) used in the CLG medium incorporated tritium from H3-thymidine into autoradiographically detectable DNase-sensitive material. Autoradiographs of amebae fed bacteria prelabeled with H3-thymidine also revealed some nuclear and cytoplasmic label. Thus, the amount of cytoplasmic label due to ingested, prelabeled bacterial DNA and/or actual biosynthesis of cytoplasmic DNA by the amebae themselves, is not known. Also, at least some of the nuclear DNA of amebae is synthesized from ingested bacteria, or, more likely, from bacterial degradation products.

Notes: SYNOPSIS. Molecular heterogeneity of malic dehydrogenase (MDH) in malaria was shown by zone electrophoresis in potato starch, starch gel, and by enzymatic activity with analogs of the coenzyme diphosphopyridine nucletide. A single anodal peak of MDH characterized the normal duck red blood cell whereas P. lophurae free of the host cell had a cathodal form of the enzyme. Infected duck erythrocytes had a combination of these electrophoretic forms. The isolated enzymes had different pH optima with oxaloacetate as substrate: pH 7.4 for the duck red cell and 6.4 for the plasmodial enzyme. The Km of each enzyme for oxaloacetate varied with the pH. The Km at pH 7.4 was 4.1 and 4.4 × 105 M for parasite and host, respectively, whereas at 6.4 it was 2.0 × 105 M for P. lophurae and 6.3 × 105M for the duck erythrocyte. At pH 7.4 both enzymes were inhibited by oxaloacetate concentrations greater than 10−4 M. P. berghei MDH also had a different electrophoretic character from that of the mouse red blood cell. Quantitatively, MDH activity increased with parasitization, and erythrocyte-free P. lophurae contained approximately twice the activity found in the uninfected duck erythrocyte. The quantity of MDH activity of the infected cell was ca. 50% less than the sum of the activities of the parasite and the uninfected cell. It is suggested that these properties of the parasite MDH may give it a physiologic advantage over the red cell under the conditions which prevail intraerythrocytically.

Notes: SYNOPSIS. The structure and certain aspects of the physiology of the sexual cycle, including mating and zygote germination, were studied in strains of the colonial green flagellate Astrephomene gubernaculifera. Mating type inheritance was analyzed in 4 crosses among strains with 7 chromosomes and 1 cross between strains with 4 chromosomes. Results in all cases indicated that mating type was under the control of a single pair of alleles.Twenty-six morphologically identical clones from separate geographic areas were studied with regard to sexual compatibility. On the basis of intercrossing, 6 sexually isolated groups were recognized. Complete gene flow (genetic compatibility) was found among the strains comprising intercrossing Group I.

Notes: SYNOPSIS. Streptomycin (SM) inhibition of greening of Euglena gracilis, strain z, was studied. The antibiotic was most effective if present during cell division in the absence of light. The next most effective condition was that which allowed cell division in the light, and the least effective conditions were those that allowed only minimal cell division in the dark or light (i.e., under “resting” conditions). In the dark, 20–200 times higher concentrations of SM were required for the same degree of inhibition under resting conditions as under growing conditions. The observation of Kirk (Biochim. Biophys. Acta, 1962, 56, 139–51)-that the pH of the resting medium influenced the degree of inhibition–was confirmed.

Notes: SYNOPSIS. Plasmodium japonicum and P. juxtanucleare are two very similar species of avian malaria parasites. The former was discovered in domestic fowl in Japan, and the latter in the same host species in Brazil; it has since been found in chickens in Uruguay, Mexico, and Ceylon. The present study, based on a Ceylon strain of P. juxtanucleare and slides from the Bamboo Partridge (Bambusicola thoracica sonorivox) of Taiwan indicates that the latter host is a natural reservoir of P. juxtanucleare, since this species appears to differ in no significant way from P. japonicum. Infection is common in the Bamboo Partridge (5 of 26 birds showed it), but it has not so far been found in any other avian species, although a total of 973 birds, belonging to 17 orders, 45 families, 110 genera, and 183 species, have been examined from this area. Since the younger stages of Plasmodium nucleophilum look very much like similar stages of P. juxtanucleare, they are compared. The older stages however are usually easily distinguishable, and the former species is so far known only from non-gallinaceous birds whereas the latter appears to be limited to gallinaceous ones.

Notes: SYNOPSIS. The presence of amino acid activating enzymes was demonstrated in the ciliated protozoan Tetrahymena pyriformis. By employing a sensitive hydroxamate assay procedure, the activation of L-valine was assayed in various subcellular fractions of the ciliate, and some characteristics of the enzyme activity in the most active fraction were determined. Most of the activity resided in pH 5 fractions isolated from high speed supernatants of ciliates disrupted by various physical and chemical methods. No activity could be demonstrated in isolated cilia, in pellicles with attached kinetosomes, in microsomes or in macronuclei, providing these organelles were thoroughly washed. A washed mitochondrial preparation isolated by the Mager and Lipmann procedure activated L-valine; mitochondria isolated by the procedure of Hogg and Kornberg did not.The pH 5 fraction isolated from the 102,000 X g supernatant of digitonin-lysed ciliates was stable for several weeks when stored in 0.1 M Tris buffer, pH 7.6 at – 25 C. The activity of this fraction with respect to L-valine activation was dependent on the presence of ATP1 and magnesium in the reaction mixture. The optimal concentrations of these components and of L-valine and hydroxylamine were determined, and the linearity of activity with time and enzyme concentration was demonstrated. Valine activation was not modified by dialysis of the pH 5 fraction, or treatment with RNase, or the addition of boiled pH 5 fraction.

Notes: SYNOPSIS. Five- to 6-day-old resting cells of Ochromonas malhamensis were incubated at pH 6.5 with glucose and appropriate C14 precursors of the methyl groups of phospholipid-choline. Under the experimental conditions L-methionine-C14H3 was the most efficient source of choline-methyl groups, followed by formate-C14, formaldehyde-C14 and DL-serine-3-C14, respectively. Glycine-2-C14 was not incorporated into choline. Both L-methionine-C14H3 and formate-C14 served as precursors for the methyl groups of monomethylethanolamine, dimethylethanolamine and choline. Addition of non-radio-active L-methionine depressed the incorporation of formate-C14 into choline-methyl groups by 50%. The results support the hypothesis that methionine can be the source of all 3 methyl groups of choline, and that formate is probably converted to the methyl group of methionine before transmethylation to choline. However, an alternate pathway from single-carbon sources cannot be excluded.

Notes: SYNOPSIS. Under the influence of ecdysone a uninucleate asexual cell of Paranotila lata gen. et sp. nov. is transformed into a gametocyte in which, as a rule, 8 successive nuclear divisions occur, thus producing 8 male and 8 female pronuclei. No cytokinesis occurs during these divisions. The process should be regarded as a gametogenesis in which only nuclei participate. These gametic pronuclei fuse, male with female, and produce 8 fusion or zygotic nuclei. The cell may now be regarded as a zygote, which soon undergoes plasmotomy, and eventually produces 8 uninucleate, diploid asexual cells. This is the usual course of events. A few exceptions or irregularities have been noted. Throughout gametogenesis, Paranotila is quite indifferent regarding the production of new flagella and axostyles; sometimes they are produced, sometimes they are not.

Notes: SYNOPSIS. The taxonomy of the genera Mallomonas, Mallomonopsis and Synura is based on their silica scales. Examination of water samples from Southern Scotland in the electron microscope revealed that they were plentiful in the area and permitted detailed observations on new species. Comparative studies are made on the flagella of three known species of Synura; the “flimmergeissel” of these biflagellates appear to be more or less similar, but the whip-lash flagella show specific differences suggesting a possible secondary taxonomic criterion. The distribution of the three genera is discussed with particular reference to species found in Iceland, Scotland and Japan. A stage in the conjugation of a species of Mallomonas is described.

Notes: SYNOPSIS. A microsporidian, Nosema muris n. sp., is described from laboratory white mice in Czechoslovakia. I t differs from Nosema cuniculi (Levaditi, Nicolau and Schoen, 1923) and other Microsporidia in mice in that it develops in the omentum and heart muscle, iorming large masses of schizonts and spores. The parasite is distributed to other tissues (liver, spleen, fat, peritoneal muscles, connective tissue and brain) by infected mononudear cells, and then forms localized cysts in them. Kidney, lungs, adrenal glands, pancreas and salivary glands are not infected. The parasite can be transmitted via the placenta, by feeding on infected tissues, or by experimental injection of ascitic fluid.

Notes: SYNOPSIS. Babesia bigemina was identified in the erythrocytes of calves as early as 9 days 21 hours after infected larval ticks (Boophilus microplus) had been placed on them. This confirms the circumstantial data [Hoyte, (1961): J. Protozool. 8, 462–61, which indicated that there is no pre-erythrocytic schizogony in the life cycle of B. bigemina.The earliest time at which parasites were themselves found to be capable of infecting ticks was between 11 and 12 days, (i.e., about two days after parasites were first seen in the blood). It is suggested that this delay occurs because, until this time, parasites are present in the blood in such small numbers that none survive digestion within the tick.

Notes: SYNOPSIS. Diophrys scutum, collected from four locations on the New Hampshire coast, ranged from 89–195 7mu; in length, 50–105 μ in width, and 68–88 μ in buccal cavity length. The end of the adoral zone of membranelles (AZM) extends 37–59 μ (average = 45.6 μ) posteriorly in a groove on the right side of the body. Dorsally are five rows of stiff cilia. The silverline system (Chatton-Llvoff technique) appears as a fine meshwork, entirely different from that found in Euplotes or Uronychia. There are two elongate macronuclei (Feulgen reaction) and several micronuclei. Diophrys peloetes n. sp., collected from one location in Alligator Harbor. Florida, ranged from 95–134 μ in length, 62–84 μ in width. and 60–80 μ in buccal cavity length. The terminal portion of the AZM extends posteriorly in a groove 30–44 7mu; (average = 36.8 μ) on the right side of the body. Dorsally are eight rows of stiff cilia. The details of the silverline system are similar to those of D. scutum.There is insufficient difference in ranges of body length, width. and buccal cavity length to use these characters in separating the two species. However, a statistical analysis shows that the length of the portion of the AZM on the right side of the body in D. scutum is significantly different (longer) from that of D. peloetes. Furthermore, these two species differ not only in number of dorsal ciliary rows, but also in the number of cilia per row. The degree of difference in these two species is similar to that between closely related species in other hypotrich genera, and also to that between some varieties of Paramecium aurelia.

Notes: SYNOPSIS. The mechanism of carbohydrate metabolism was studied in homogenates of the ameba, Mayorella palestinensis. The utilization of intermediates with the formation of lactic acid as the end product of the anaerobic breakdown of the carbohydrates was established. Evidence was obtained for the presence of the main enzymes associated with the Embden-Meyerhof glycolytic scheme. The activity of hexokinase and phosphorylase was ascertained in a M. pakstinensis homogenate. These enzymes have not yet been found among other soil amebas grown in axenic cultures.

Notes: SYNOPSIS. Chloramphenicol inhibits growth of Euglena gracilis. It also interferes in the “greening” process by inhibiting protein synthesis (measured by leucine uptake), but RNA synthesis is essentially unafiected. The antibiotic acts on all cell fractions, but prefermtially inhibits the synthesis of plastid protein. Inhibition of chlorophyll synthesis is transitory and does not affect the self-reproduction system for chloroplast formation.

Notes: SYNOPSIS. Thirty to 80% of Tetramitus rostratus amoebae were induced to transform into the flagellated stage. The procedure involved growth in a dilute hydrolyzed casein medium with living Escherichia coli dispensed in microdrops approximately 0.5 mm3 in volume and sealed in a slide-depressionchamber. Single amoebae placed in such microdrops did not transform until growth and division had produced a population density of 100 amoebae/mm3. Flagellation then began and proceeded rapidly.Analysis of growth and transformation in microdrops of different sizes but with equal numbers of amoebae, or of microdrops of the same size but different numbers of amoebae, suggested that the amoebae themselves were elaborating substances into the medium which brought about their own transformation. The effects of bacteriostasis induced by a variety of antibiotics did not clearly rule out a bacterial contribution to the factors causing transformation. Osmotic effects within physiological limits did not play any demonstrable role in inducing the response.It was concluded that transformation of amoebae to flagellates involved rapid growth and subsequent undetermined changes in the medium.

Notes: SYNOPSIS. Thymidine-methyl-H2 is incorporated into the cytoplasm of Euglena. The label is non-nuclear and not in DNA; evidence for its presence in RNA and protein is presented. Only Euglena which maintain the potentiality to develop chloroplasts show this incorporation; it was not observed in streptomycin. uv, benadryl, O-methyl threonine or heat “bleached”Euglena, nor in Astasia longa.Preliminary incorporation experiments show that exogenous pyrimidines are not utilized as nucleic acid precursors in Euglena in general. However, the tritiated purines are incorporated into DNA and RNA. The use of thymidine to localize DNA autoradiographically in Euglena is completely excluded.

Notes: SYNOPSIS. A survey of the genus Lagenophrys Stein, 1852 (Ciliata, Peritricha) on freshwater crayfish (Parastacidae) revealed a wide range of new species in Australia and New Zealand. Fourteen new species are described. L. seticola sp. nov. is close to L. vaginicola Stein. L. spinosa, L. bispinosa, L. latispinosa, L. communis, L. occlusa, L. lawrii, L. lingulata spp. nov. form one species group. L. willisi, L. rugosa, L. darwini, L. deserti, L. ditngogi and L. engaei spp. nov. form a second group distinguished by characters of the lorica, lorica mouth and macronucleus.Nenninger's classification of degree of specificity of commensal-host relationship is applied to the whole genus and expanded in the light of the Australian material. The zoogeographic problems resulting from the specificity of Lagenophrys and the limited range of some of its hosts are indicated.

Notes: SYNOPSIS. Blood films made from the “shoveller duck”, Anas clypeata Linnaeus 1758, in Lin-pien Ping-tung Hsien, Taiwan, showed a new species of avian malaria parasite, for which the name Plasmodium anasurn is proposed. This species is characterized by elongate gametocytes often closely resembling Huemopvoteus, about 15 merozoites per segmenter (although such stages were too few for really accurate counts), and trophozoites and schizonts which very often surround the extremities of the host cell nucleus in U-shaped fashion. Pigment is abundant, appears early, and is very dark. The host cell shows little or no alteration. No infected reticulocytes or blood cells other than erythrocytes were observed.

Notes: This article deals in an axiomatic manner with problems of definition, classification, and measurement in the national accounts. It argues that the elementary units which must be classified in national accounting are economic objects (real and financial), rather than transactions. The article defines briefly a set of postulates, and shows that the structure of a simple system of national accounting can be derived from them. There are twenty postulates—certain of them establishing basic categories such as sector, time, economic object, value (price); others establishing relations between categories (for example the notion of ownership); and others describing operations in which economic objects can be involved, such as production, final consumption, change of ownership, and change of debtor and creditor (in the case of financial objects). It is shown that the system of postulates makes it possible to consider a large number of accounting concepts (flows or stocks) as classes (baskets) of real objects (e.g., exports, real capital) or financial objects (e.g., payments, total debt of a sector). These concepts can be defined without reference to prices, although prices are necessary to measure them. Other concepts cannot be defined in this way in this system of postulates, for example value added, foreign balance, saving, net worth. However, it is possible to define magnitudes of the latter type and measure them in terms of value: for example, value added can be defined as the difference between the value of receipts and the value of outlays of a sector. In this way it is possible to establish algebraic relations among the national accounting concepts. (This article is a summary of certain parts of the doctoral thesis of the author, published in Norwegian in 1955.)

Notes: It is only within the last two years that the published United Kingdom accounts have been extended to include sector financial accounts; their use for market analysis is, therefore, still in its infancy.The sectors and sub-sectors distinguished in the financial accounts agree very closely with those recommended by the Working Group on Financial Statistics of the Conference of European Statisticians. A major difference is that in the United Kingdom accounts particular emphasis is placed on the distinction between the public sector and the private sector. For this purpose the public sector consists not only of general government but also includes public corporations (that is, public non-financial corporate enterprises). The classification of assets and liabilities is based on a general list which is also similar to that developed by the Conference of European Statisticians.Because of the large capital formation of public corporations and local authorities, the public sector is normally a substantial borrower from the pirvate sector, although its borrowing requirements fluctuate considerably from quarter to quarter because of the uneven incidence of tax receipts. The personal sector provides about one-third of the total saving of the economy, much of which is in the form of contractual saving—through life assurance and superannuation funds and the repayment of house purchase loans. No direct information is available about transactions in stocks and shares by the personal sector, but it is estimated that the sector is a very large seller of securities and in recent years its sales have amounted to £700 million a year.One factor which is important in the analysis of financial accounts and which is not shown specifically as part of the system is the rate of interest. The proportion of personal saving going into the different forms of short term assets has tended to vary according to the relative rate of interest received. The rate of interest also affects the pattern of borrowing by public authorities.The United Kingdom prepares short term forecasts of national income and of the balance of payments, and also forecasts of the borrowing requirement of the public sector and of the central government in particular. For internal purposes, forecasts are made of the various ways in which the government is expected to finance its borrowing requirement. These forecasts provide a useful framework for considering monetary prospects and are particularly important for showing the relation between the forecasts of the balance of payments and of government borrowing from domestic sources, especially from the banks.

Notes: After a short introduction, the first part of this paper (section 3 through 9) provides an outline of the revisions proposed to the System of National Accounts (SNA) of the United Nations which are now under discussion. These proposals were considered by an expert group at the end of 1964 and were accepted by the Statistical Commission of the United Nations in 1965 as the basis for further work on the extension and revision of the SNA. The aim of the revision is to provide a fully integrated system of accounts and balance sheets in which input-output, flows-of-funds and sector balance sheets are incorporated in a generalised accounting framework. Whereas the real side of the economy has been studied analytically in many countries (input-output analysis, demand analysis and so on) much less experience is available on modelling the financial side of the economy, apart from econometric work on saving behaviour, which is fairly widespread. Accordingly, the second part of the paper (sections 10 through 14) contains a discussion of financial model-building in which a number of possibilities are explored. The final topic discussed (section 15) is demographic accounting, by which is meant a framework for recording and analysing human, as opposed to economic, flows and stocks. The development of such a system arose out of the emphasis placed by the expert group on the integration of demographic and economic information.

Notes: This paper is essentially a summary of the book Measuring the Nation's Wealth (Volume 29, Studies in Income and Wealth, New York: National Bureau of Economic Research, 1964), which is the report of study directed by the author. The purpose o f the study was to assess the problems and possibilities of conducting a national census of real wealth as a basis for continuing wealth and balance sheet estimates for the U.S. economy, by major sector.It is stressed that the balance sheets and wealth estimates should be designed as a consistent part of an integrated system of national income accounts. Thus, valuation (at market prices and/or depreciated replacement costs), sectoring, and type-of-asset detail in the basic data and derived estimates should be compatible with the flow estimates contained in the economic accounts. Consistency of stock and flow estimates facilitates analysis of inter-relationships, and is helpful in the estimation process.It is recommended that in the U.S. asset data by broad categories be collected as part of the recurring economic censuses and other reporting systems, but that detail on fixed reproducible assets (construction and equipment) at cost, by year or period of acquisition, be obtained from a small sample of respondents in each industry. The detail would be useful in its own right, and also permit revaluation of the assets by use of price indexes and depreciation rates to a current depreciated replacement cost basis. Where feasible, respondent estimates of market values would also be obtained.The proposal is thus a compromise between the Japanese 1955 sample survey of assets, and the detailed wealth inventory of the U.S.S.R. which was begun in 1959. Preliminary work is now underway in the U.S. federal statistical agencies to expand collection of asset data, and to prepare comprehensive wealth estimates in the framework of the national income accounts.

Notes: Ľutilisation du SCN actuel pour les besoins de la coopération économique internationale est considérée ci-après à la lumière des expériences accumulées au sein de la Communauté Economique Européenne.Ľexpérience des années récentes prouve que si, dans une première étape, le SCN a pu servir de cadre général ďanalyse économique, ce cadre s'est rapidement révélé insuffisant lorsqu'il s'est agi de confronter des structures et des politiques nationales ou de définir des politiques coordonnées dans les domaines économique, social ou financier. Les travaux menés dans différents domaines ont montré la nécessitéďétendre, de détailler, de modifier et de préciser le système actuel de comptabilité nationale.Une question importante concerne ľintérêt de faire apparaître ou ďéliminer du système comptable les différences institutionnelles existant entre pays. Bien qu'à cet égard ľavis des utilisateurs ne soit pas toujours unanime, ľexpérience plaide en faveur ďun système reflétant pleinement les différences réelles de structure entre pays, mais suffisamment détaillé afin de permettre les regroupements fonctionnels nécessaires à certaines analyses.Ľarticle se termine par un bref rappel des principales critiques adressées au SCN actuel par ceux qui, dans le cadre de la Communauté Economique Européenne, se servent de la comptabilité nationale, critiques auxquelles la révision du SCN apportera, espérons-le, une réponse satisfaisante.

Notes: The author believes that the theoretical controversies relating to the two concepts of production have reached a dead end, and that the answer to these questions must be sought in the results of empirical research. It is to this end that he presents the results which he has arrived at in his computation of Yugoslav national income according to the Yugoslav methodology, which shares the material product concept, and the methodology of the United Nations, which is typical of the enlarged concept of production.In comparing the results of the computations according to the two methodologies, the author concludes that in spite of conceptual differences the deviation of the Yugoslav estimates from the values calculated following the system of the UN is relatively modest (4.2%) if domestic product at factor cost is used. The difference relative to aggregates calculated at market price rises to 13.6%. The author explains these differences by the fact that all the Yugoslav aggregates are computed at market prices, and that services financed by budgetary contributions, which amount to 80% of all services, have found their place in the market prices of material production. He finds that the choice of prices used for calculation has a greater weight for the computation of production than conceptual differences in the methodology of the computations.

Notes: This paper is concerned with the influence of different estimation procedures on the data for real output by industry group of a number of O.E.C.D. countries. The authors have examined the methods and indicators used in preparing sector real output data and have tried to assess the effect of the different methods on the recorded changes of sector real output. The data for real output, employment and productivity are compared for the different sectors and countries. The comparison between sectors lays particular emphasis on the dichotomy between the services and non-service sectors of the economy. In this comparison as well as in inter-country comparison it is seen that the data are influenced to a considerable extent by different methods. The survey of estimation methods also shows the incidence of use of double deflation techniques and other methods in the different countries, and the extent to which quality change, output specification and valuation problems are reflected in the different methods.

Notes: The purpose of this paper is to describe and evaluate the quarterly national income and product accounts of the United States (to be referred to henceforth as N.I.P.). The historical development of these accounts is reviewed first. Next, a summary of the statistical methodology underlying them is provided. An analysis of the errors to which they have been subject follows. An attempt is then made to define the area of their usefulness. Finally, suggestions for their improvement are formulated.The most general conclusions are as follows:〈list xml:id="l1" style="custom"〉1The quarterly N.I.P. estimates were developed in close response to an urgent need for them in economic analysis and policy formulation. Their development was a gradual process covering about two decades in which experience gained at one stage suggested improvements and extensions for the next.2The quarterly figures are based on abundant data sources. To be sure, the information is not as comprehensive as that available for the preparation of the annual estimates, but we are dealing only with differences in degree - there is no sharp contrast.3The series are subject to a considerable margin of error. There is bias in some of them - the initial estimates tend to be too low; and quite apart from bias they differ from the revised figures.4The margin of error attaching to the statistics disqualifies them from serving as precision instruments, but they are indispensable as a systematic framework for the order-of-magnitude analysis of the major forces determining the short-run movements of the economy. At present, the outstanding handicap in such analysis is not statistical error, but the inadequacy of economic theories and of our ability to test them.5The opportunities for mechanizing estimating techniques are assessed; the work on seasonals is discussed in this context; and a thorough investigation is proposed of the problems involved in the synchronization of the various components of the accounts. In addition, proposals for improving the data available for the estimation of specific income and product flows are formulated. Quarterly estimates of the physical volume of national output by industry, and quarterly accounts showing the financial transactions that mediate between saving and tangible investment are considered the two high-priority major extensions of the present accounts. Other, less basic, extensions of these accounts are also proposed.

Notes: This paper examines differentials in output, employment and productivity across seventeen service industries in the United States from 1939 to 1963. Included are 9 retail trades and 8 services mostly from the personal service group. The industries chosen were those for which it was possible to obtain from available data reasonably comparable measures of output and input for selected years since 1939. Also, they are industries for which it is possible to calculate a measure of real output that is not based on labor input.Sixteen of the industries show positive rates of change of real output per man. Thus there appears to be no basis for assuming that productivity cannot or does not increase in industries providing services. However, the rate of increase for the group as a whole was not as rapid as in manufacturing or in goods production as a whole.The data for the seventeen industries give strong support to the hypothesis of a positive correlation between industry rates of growth and rates of change of productivity. The correlations are of the same order of magnitude as those found by other investigators in studies of manufacturing industries.The coefficient of correlation between growth of output per man and growth of output is .93; between growth of output per man and growth of employment it is .54 for 1939–1963. The comparable coefficients for the 1948–1963 period are .70 and .13.The results also parallel those reported for manufacturing in one other respect, namely, the absence of any correlation between changes in output per man and changes in compensation per man.The paper concludes with a discussion of the problems encountered in measuring changes in real output in these industries and presents some alternative estimates based on different concepts and different sources.

Notes: After an introduction setting out the general state of work on the national accounts in the Middle East the author considers the principal uses of national accounts statistics in less developed countries. The first group of uses discussed is in connexion with the measurement of growth and the making of international comparisons. The author is of the opinion that in many cases the primary statistical series are so weak that the fact they they are combined together into a series called national income or gross domestic product lends to them a significance which they do not really possess. The real problem is to improve the quality of the primary series.A second use of national accounts statistics is in connexion with fiscal and budgetary policy. In the statistically advanced countries this is one of the most important uses but in the less developed countries budgetary policy has not yet reached a level of sophistication which would call for the use of national accounts data. Moreover, the time factor involved in assembling accurate national accounts estimates militates against their effective use for short term forecasting.The author considers that the most important use for national accounts statistics is to provide a framework for development planning. The United Nations system is not altogether appropriate for this purpose. It grew up primarily as a system for recording income flows but in development planning one is concerned equally with commodity flows with a great deal of attention being focussed upon intermediate products. The proposals of the working group of African Statisticians for an adaptation of the S.N.A. to African countries represents a most important advance in this respect.In the final section of the paper the author advocates a broader definition of capital formation to include developmental expenditure which is not properly defined as fixed capital formation. Education expenditure is cited as an example. It is suggested that in the national accounts it would be desirable to operate with gross concepts. However, the growth of the capital stock is obviously important in less developed countries and it is suggested that statistical techniques be devised to measure it directly wherever possible. Finally, attention is drawn to the ambiguities and weaknesses in the concept of residence as used at present in the S.N.A.

Notes: Cet article examine comment les calculateurs modernes servent ou pourraient servir en comptabilité nationale, soit pour ľutilisation des comptes, soit pour leur préparation.Du premier point de vue ľauteur insiste ďabord sur la complexité croissante des comptes nationaux qui rend difficile leur usage à partir de la forme classique de tableaux imprimés et tend à rendre nécessaire leur transcription sous forme de cartes perforées, cequi permettra de faire à la machine un certain nombre de travaux élémentaires sur les comptes.On peut également penser que les calculateurs permettraient des sondages rapides sur certains éléments des comptes en cours de préparation.Enfin, les utilisations des calculateurs électroniques pour ľanalyse des comptes, ľétude des modèles de projection et leur résolution sont bien connues.En ce qui concerne ľutilisation des calculateurs pour la préparation des comptes ľauteur montre que, étant donné le caractère imparfait du système statistique dans de nombreux pays, les comptes économiques des années passées sont en partie préparés àľaide de véritables modèles qui permettent de passer des données de base aux comptes et qui peuvent dans certains cas être résolus àľaide de calculateurs électroniques.Là oùľinformation statistique est meilleure, des éléments des comptes peuvent être calculés directement par agrégation des données élémentaires. On peut donc penser qu'une époque viendra où la plus grande partie des comptes nationaux sera préparée àľaide de machines électroniques à partir des données de base.On peut alors imaginer un monde où les transactions élémentaires seraient enregistrées au moment où elles seraient réalisées, rendant ainsi entièrement automatique la préparation des comptes nationaux. Mais alors les machines pourraient réunir les modèles de préparation et les modèles ďutilisation des comptes. Ľétape intermédiaire que constitue la préparation et la publication des comptes sous la forme actuelle deviendrait alors inutile.

Notes: The paper discusses national balance sheets in the light of the proposals for their inclusion in the revised SNA. The author uses his own estimated national balance sheet of the United Kingdom as a basis for discussing the problems encountered in the compilation of national balance sheets, and the greater part of the paper is concerned with questions of valuation, classification and statistical source material. The concluding section deals briefly with the structure of the national balance sheet of the United Kingdom and compares its structure with that of the national balance sheet of the United States. Provisional national balance sheets of the United Kingdom for each of the five years 1957 to 1961 are presented.

Notes: Both the yearly and the quarterly national accounts of Norway are derived with intensive use of electronic computers, punchcards and magnetic tapes.The introduction of the paper gives a short description of the Norwegian national accounting system. Four aspects are stressed. First, the accounts are built up by the production approach with the main purpose of, on the one hand, showing the domestic product at market prices by industry of origin and, on the other hand, showing a detailed breakdown by categories of expenditure. Second, the main part of the system consists of an input/output matrix of about 1700 commodities, 130 industries, and 150 final demand sectors. The input/output table is thus not made separately but is the main body of the national accounts. Third, this annual input/output table is used as a basis for estimating quarterly accounts. Fourth, an econometric model for analysing and forecasting developed by the Norwegian Central Bureau of Statistics is closely linked to the national accounts.The paper concentrates on how the computers and punchcards are used. It describes how all available information on the supply and use of commodities, prices, etc., is brought together. As far as possible punchcards from the basic statistics are taken over for further processing. As the end result each flow in the national accounts is represented by a punchcard.The paper discusses what has been gained by switching from worksheets to punchcards. There are advantages during the work and there are advantages of having the final results on punchcards.The final sections describe how the punched cards for the yearly accounts data are used as basis for preliminary estimates of quarterly accounts and also in a model for forecasting.

Notes: (1) The primary contribution from the computer's application to the national accounts may well be to erode the line between micro and macro analysis. Key macro totals in the accounts sum individual company reports. The computer permits us to develop distributions of these reports. Such distributions, regularly presented, would permit discovery of the first forerunners of change, would help distinguish, e.g., widespread strength in an export drive or a profits surge, from participation by a few major concerns that dominate the aggregate.(2) The strikingly different parameters in cross section and time series studies (e.g., price elasticity of housing) will in some measure reflect incomparability between the micro data that enter into each. The computer makes possible the use of the wide array of micro data that really underly the accounts to develop consistent analyses of time series (of both aggregates and distributions) and cross section analyses.(3) The inconsistencies now imbedded in the accounts but gilded over by the abilities of the estimators are well-known. Discussions of wage price policy rest on data for wages that have no necessary compatibility with data on profits, etc. But since 1,500 corporations account for at least half of U.S. net income, sales, and investment, the computer can test the consistency of reports made by different units in these firms to different agencies—a process totally out of the question before the computer.(4) The potential that the computer offers for prompt revisions in the accounts; for revisions by systematic rule; for tests of sensitivity of the entire set of accounts to particular tailor-made adjustments, is clear.(5) Company purchase orders and accounts are increasingly recorded on cards or tapes. From these we may derive input-output detail and process detail that are light years better than those now feasible from intermittent survey aggregates.

Notes: It is argued that the conventions of an accounting system, such as the S.N.A., are a matter of convenience. The treatment of education as a current expenditure, instead of as a form of capital formation, derives from the Keynesian system, and is not appropriate for dynamic problems of developing countries, where weaknesses in education are often the main “bottleneck” in the process of development. In such countries, expenditure on education clearly yields its benefits mainly in the longer run. To treat this as a consumption item biases policy in the direction of using financial resources for fixed capital rather than human investment, and may cause aid agencies to penalize countries which expand their educational systems. A similar problem arises on other expenditures such as health, but the case for treating them as investment is not so strong.To treat educational expenditure as part of capital formation logically requires two major changes. First education needs to be removed from private and public consumption, and for this purpose a fairly broad definition of what is education should be used. Secondly, the stock of educational capital should be valued. The valuation problems are, however, severe. Variations in cost components make historic cost of little value as a yardstick, and calculations of future returns are fraught with difficulties. Using replacement costs, which seems the best method, involves the construction of education profiles in physical terms which can then be valued by present or by standardised costs. The depreciation of human capital through mortality and retirement can be allowed for by applying national average rates to these physical profiles.Switching educational expenditure from current to capital accounts involves no serious practical problem. However, although there should logically be an allowance for depreciation on human capital, this is not recommended; single monetary measures of educational stock are not very meaningful, and this would involve changing the definition of “net” aggregates. Development of statistics of educational stocks and flows in physical terms—the beginnings of “demographic accounting” fully integrated with the rest of national accounting—is strongly advocated.

Notes: The now urgent problem in the field is to translate into practice the theoretical agreement, slowly reached over the past two decades, on the need for and feasibility of sectorized national balance sheets.The paper discusses the five main uses of national balance sheets, viz. (1) the study of the relations among assets and liabilities at one point of time in one country, particularly the position of financial institutions; (2) the analysis of changes in one country's financial structure between several balance sheet dates; (3) the comparison of balance sheet structure at one date among two or more countries; (4) the comparison of the financial development of several countries for at least two but usually more numerous balance sheet dates; and (5) the use of selected balance sheet items, e.g., reproducible tangible assets or liquid financial assets, in econometric models.Examples are presented of the first three uses, viz. for (1) an eleven sector balance sheet matrix for the U.S. as of the end of 1962; for (2) an unsectored national balance sheet of the U.S. in 1900, 1912, 1929, 1939, 1945 and 1958; and for (3) a comparison of condensed unsectored national balance sheets for a dozen countries (USA, UK, France, Germany, Belgium, Norway, Italy, Japan, Israel, Mexico, India and the USSR) for a date in the neighborhood of 1960.

Notes: Spurious reflections showing a residual move-out are generally present on both corrected seismic cross-sections and common depth point composites.We propose to determine a space-time filter satisfying the following conditions: to attenuate as much as possible reflections presenting a given move-out, to retain intact reflections whose time gradient is zero (or has a predetermined value), to be applicable efficiently even to a small number of traces, not to amplify random noise unduly.After briefly indicating the design principle of these filters, we shall give the results of their application to the theoretical examples, in order to bring out: the influence of sampling interval and filter length (possibility of achieving efficient filtering using an apparatus with a small number of terms), the effect of filtering on dipping reflections (reduction in amplitude and distortion increasing with the time gradient), the range of reflection move-out that can effectively be filtered with the same apparatus, the disturbing influence of random noise.〈section xml:id="abs1-2"〉〈title type="main"〉RESUMEOn constate généralement, tant sur les films sismiques corrigés que sur les résultats de couverture multiple, la présence de réflexions parasites présentant une courbure résiduelle d'indicatrice.Nous nous sommes proposé de déterminer un opérateur de filtrage spatio-temporel satisfaisant aux conditions suivantes: filtrer le plus possible les réflexions présentant une courbure donnée, conserver intégralement les réflexions dont le gradient temps est nul (ou a une valeur déterminée), pourvoir être appliqué efficacement à un petit nombre de traces, ne pas amplifier outre mesure les bruits inorganicés.Après avoir indiqué brièvement le principe du calcul de ces filtres, nous présenterons les résultats de leur application à des exemples théoriques, afin de mettre en évidence:ľ'influence du pas d'échantillonnage et de la longueur du filtre (possibilityé d'obtenir un filtrage efficace avec un opérateur comportant un petit nombre de termes),ľ'effet du filtrage sur les réflexions pentées (réduction d'amplitude et distorsion croissant avec le gradient temps),ľ'ouverture de ľ'éventail des réflexions courbes que ľ'on peut filtrer efficacement avec un même opérateur,ľ'influence perturbatrice de bruits inorganicés.

Notes: An iterative process is proposed that computes a distribution of masses giving rise to a certain gravitational field. The possibility of applying a similar procedure to magnetic and electric fields is also discussed.

Notes: The method presented takes the influence of refraction rigidly into account. All travel-times available are used in an application of the method of least squares. The solution is illustrated by a computer flow diagram.

Notes: Charts are presented for the computation of the vertical component of the attraction of horizontal laminas and of horizontal cylinders bounded by irregular w-sided polygons. Contrary to older methods no restricting conditions are made for the directions of the sides of the polygons.

Notes: A theory is developed to predict the amplitude and velocity of the first arrival produced by a thin solid refractor embedded in a solid environment. Laboratory experiments on a three dimensional model substantially confirm the theory. The essential conclusion may be found in Figure 8 where it is shown that for a constant wavelength, and for thicknesses up to about one half a wavelength, the attenuation per unit distance decreases monotonically with increase of refractor thickness.The assumption is made that the amplitude, A, attenuates as (x)-n exp(-βx), where x is horizontal distance and n and β are constants. It is emphasised that it is difficult to obtain reliable values for n and β from the initial amplitude measurements.

Notes: Book reviewed in this article: State of Stress in the Earth's Crust, Proceedings of the International ConferenceF. A. Vening Meinesz, The Earth's Crust and Mantle (Development in Solid Earth Geophysics)

Notes: Book reviewed in this article:M. R. J. Wyllie, The Fundamentals of Well Log Interpretation.

Notes: Proton precession magnetometers usually operate discontinuously since thy rely one intermittent impulses. The aerial versions of these magnetometers suffer from relatively high background noise. The type of magnetometer discussed in this paper has solved the two problems of discontinuity of measurements and reduced sensitivity due to background noise.Comparative texts with an established flux-gate apparatus have given significant and convincing results.

Notes: Book reviewed in this article:S. I. Kuznetsov, M. V. Ivanov and N. N. Lyalikova, Introduction to Geological Microbiology Organic Geochemistry, Editor I. A. Breger

Notes: The formula for the Bouguer anomaly contains the density of the masses above the datum plane. If we use a wrong value for the density we obtain on the Bouguer map additional anomalies which coincide with the topographic features.If we do not know the Bouguer density we can compute several Bouguer maps based on different arbitrary densities. Then we select that map which shows the least correlation with the topography and obtain in this way a reasonable value for the density. This method has been found by Nettleton.Several authors have formulated this process mathematically and have elaborated numerical methods. This paper will present such a method. It differs from the former methods by the following:〈list xml:id="l1" style="custom"〉1A general formula for the Bouguer density is presented, using a non-specified linear operator. By choosing special definition for this operator, we can obtain the formulae of several of the known methods.2It is shown that the determination of the density by “Nettleton Profiles” gives less reliable results than the statistical investigation of the whole area covered by the gravity survey.3The known methods do not consider the variations of the vertical gradient. It is shown that in areas with a regional gravity anomaly or an extended topographic feature the known methods yield poor results.4It is shown that the vertical gradient of gravity used as operator gives a most exact and simple formula.5It is shown how the electronic computer—after reading the Bouguer values based on an arbitrary initial density—can compute the residuals, derivatives etc., which are based on the corrected Bouguer density.

Notes: An electronic analogue was used to obtain geophone distortion of typical seismic pulses. Some of the results were checked by computation and in all cases agreement was extremely close. For late arrival events estimates of attenuation and phase shift may be made from the steady-state response curves. It is explained why these curves are not applicable to the first peak, for which one must use the results in Table III.Ground-geophone coupling introduces the same distortion as a very lightly damped galvanometer with a natural frequency usually in the range 100c/s—200c/s. In swampy terrain this frequency may reduce to 30c/s—40c/s and coupling effects may be severe.An electronic unit whose characteristics are the inverse of those of a geophone has been used to improve the first peak amplitude of a seismic refraction record. Since 1c/s geophones are often desirable in refraction survey it may well be more economic to use higher frequency geophones followed by their inverse filter.

Notes: The paper describes a new method for the calculation of standard-graphs i.e. resistivity-curves for assumed resistivity profiles. It starts from the integral of Stefanesco and arrives at a system of image poles, similar to, but simpler than earlier developed systems. The advantages of the new method are described, the accuracy is discussed and it is indicated how the accuracy can be improved by a simple artifice.

Notes: In the present study an attempt is made to relate the general pattern of reflection seismograms obtained over a coal basin to the acoustical properties peculiar to the Coal Measures. The Carboniferous is characterized by a very rapid succession of distinct lithological units, resulting in a surprising variability in their physical properties. Coal seams in particular are conspicuous by their low density and velocity and they produce acoustical contrasts of 35–50 % with respect to country rock. The resulting heavy shielding action prevents the seismic energy from penetrating deeply into this type of formation.With the aid of synthetic seismograms, arranged into synthetic profiles, it is shown that only a minor part of the Coal Measures reflections is of primary origin. The majority has a secondary character brought about by reverberations inside the weathering zone. Thus the conclusion is reached that reflection shooting allows of mapping only the structure of the top section of the Carboniferous, which is sufficient for the purposes of the coalmining industry.

Notes: A permanent pasture was ploughed, 5 rates of potassium were incorporated in the seed-bed and a timothy/white-clover seed mixture was sown direct without a companion crop. Plant counts were taken during the fourth week of establishment. The highest rate of potash increased the size and number of timothy/white-clover plants considerably, without altering the timothy/white-clover ratio. Half the plots were cut once during the seeding year and the other half twice. Increasing rates of K increased dry-matter yields, particularly on the plots cut 7 weeks after sowing. Botanical analysis in the following year showed that, under both cutting systems, increasing rates of K tended to increase the proportion of white clover and decrease that of unsown species.

Notes: Book reviewed in this artilce:THRAN, P.; BROEKHUIZEN, S. Agro-ecological atlas of cereal growing in EuropeTHE READER'S DIGEST ASSOC. [Ed.] Complete atlas of the British IslesM. A.F. F. At the Farmer's service.

Notes: Two experiments were conducted to study the effect on nutrient loss and feeding value of making hay by different means, using a barn-drier, a forage-harvester, a crimper and conventional tedding. Both forage-harvesting and crimping of the herbage significantly increased the rate of drying, compared with tedding alone. In Exp. 2 this effect enabled the crimped and forage-harvested hays to be baled for storage 3 days earlier than the tedded hay. The use of the forage-harvester significantly reduced the yield of herbage compared with conventional mowing. Nutrient losses from cutting until storage were highest for the forage-harvested hays, the loss of dry matter between cutting and storage in the forage-harvested hays being 39·9 and 19·3%, respectively, in Expt 1 and 2 with corresponding losses in the conventionally made hays of 7·1% and 9·3%. Crimping the hay resulted in higher nutrient losses than tedding. In Expt 2 there was a loss of dry matter of 17·5% in the conventionally made hay during an 18-week storage period, compared with 3·6% in barn-dried hay, 10·8% in forage-harvested hay and 8·8% in crimped hay.In Expt 2, in which digestibility determinations were made, conservation decreased the total digestible nutrients and starch equivalent in all treatments. The use of the barn-drier gave the most efficient conservation of total digestible nutrients, followed by the crimped, forage-harvested and conventional treatments in that order. The starch equivalent values of hays made from the same sward were 43·0 when barn-dried, 39·8 when crimped, 38·6 after forage-harvesting and 31·6 after tedding.

Notes: Two different sizes of seed of a tetraploid and a diploid Italian ryegrass were sown in pure stands. Harvests were made at frequent intervals from 14 to 54 days after sowing, the root-and shoot-yields and numbers of leaves and tillers being recorded at each harvest. The results suggest that seed size per se has little influence on leaf- and tiller-numbers and that large seed increases yield potential only during the first few weeks of growth.

Notes: In a survey in the West of Scotland, utilized-starch-equivalent (U. S.E.) outputs from the grassland of 67 farms, mainly dairy farms, and 9 hill-grazing units associated with them, were assessed by the farm-recording method. The limitations and possibilities of the method are discussed. The results obtained were above average, but the farms were a selected group. The wide range of U. S.E. outputs from farms of similar type indicates the scope for improvement on farms with low levels of output. The need for more surveys to clarify existing production levels on commercial farms is emphasized.

Notes: Three experiments examine the effects of giving small supplements of flaked maize to steers on the digestibility and retention of nutrients from rations of grass silage, lucerne hay and fresh lucerne. The digestibility of the dry matter, crude fibre and crude protein of the silage ration was depressed by the successive additions of 1, 2 or 3 Ib flaked maize per day. There was a tendency for the steers to retain more nitrogen as the level of starch supplementation increased, in spite of a lowered crude-protein digestibility. The digestibilities of the constituents of lucerne hay and fresh lucerne were slightly increased by the addition of 2 and 4 Ib flaked maize per day. The reasons are discussed for the different effect of the supplemental starch on the digestibility of the crude fibre from grass silage and from fresh or cured lucerne. Attention is drawn to the increase in the retention of N following the supplementation of silage with starch.

Notes: Seven species or varieties of grass, and a mixture of 3 of them, were sown in pure swards and treated with 4 levels of nitrogenous fertilizer (0,17·5, 35 and 70lb N per acre per cut). Each species, and the mixture, was also sown with white clover. The experiment was cut 4 or 5 times per year. The effect of fertilizer on the yield of each grass was compared with the effect of clover on the yield of the grass/clover swards. Mean annual yields showed an approximately linear response to N; there was a small but significant fall in response to the highest level of N. Response among the species ranged from 20 to 30 Ib of dry matter per Ib N applied for the intermediate level of fertilizer and from 14 to 23 Ib for the final increment of fertilizer.S37 cocksfoot, S48 timothy, S24 ryegrass, and a mixture of these grasses, were high yielding and responded well to fertilizer N; Irish ryegrass and Agrostis tenuis were less productive and gave poorer responses to N. S215 meadow fescue and S23 ryegrass were intermediate in yield and response.There were no significant differences between the annual yields of the 8 grass/clover mixtures; the yields of the grass and clover components of each mixture were inversely related. The effect of clover on the yield of the grass/clover mixtures was estimated to be equivalent to the effect of an annual application of 205 lb N per acre to Agrostis tenuis and 120 lb N to S48 timothy. The fluctuations in annual yields were greater with grass/ clover mixtures than with grass swards receiving N.The yields of grasses when sown with clover were in similar order to their yields when sown pure; but whereas the latter tended to fall from year to year, the yields of the grass components of mixtures (except Irish ryegrass) did not.

Notes: The effects were studied of nitrogen and phosphorus fertilizers on the production of perennial ryegrass at high altitudes in Kenya. N had little influence on production during establishment but increased it markedly during the second and third years. The effect of P was less and was related to the amount of N applied. The response to applied N in terms of dry matter production, and the recovery of the N applied, compared favourably with data from trials elsewhere. It is apparent from the results that temperate grasses can be productive at high altitudes in Kenya and provide a considerable potential for pasture production.

Notes: As a preliminary step in the development of improved bred varieties an examination has been made of the factors which contribute to the success or failure of white clover (Trifolium repens) in hill land reseeding. Severe restrictions are placed on clover performance and N fixation in many hill areas in Britain by the poor climate, the low pH and exchangeable base status of the soils and the low frequency of effective indigenous Rhizobium strains.Agronomic solutions to some of these problems exist, but experience in mid-Wales suggests the need for new bred varieties with improved adaptation to the prevailing conditions, particular attention being directed to the limitation of N fixation by low soil temperature.

Notes: Seed shedding was studied in 50 clones each of S5I and S48 timothy during 1961–3. Some clones retained on average during 2 or 3 seasons from 80–90% of their maximum seed weight until 42 days after peak anthesis; this was up to 28% above the varietal mean. The results suggest that clones which retain their seed also produce high yields of seed and of dry matter; they can also be easily threshed.

Notes: It has been found that the rate of herbage production from perennial grass swards, cut at a stage suitable for grazing, is lower in midsummer than in late spring. This occurs even with an abundant supply of water and fertilizer. The growth rate of green material was measured in swards differing in leaf area and light interception, Results did not suggest that higher growth rates could be obtained by attention to this relationship. Examination of the tiller population of grass swards showed that, in summer, herbage yields from cocksfoot and timothy were correlated with the average tiller size. This was not so in either of 2 varieties of perennial ryegrass. During May, June and July there were changes in the erectness of the swards as measured by the proportion of the crop which could be harvested with a mowing machine. This proportion was maximal when the highest rates of production of herbage were recorded. The importance is discussed of the change from a phase when mature tillers are forming inflorescences to one when herbage is derived from young, vegetative tillers formed in the same summer.

Notes: Trials are described in which the intake of silage of varying length was determined. The results indicate that a higher voluntary dry-matter intake occurred as the length of the silage became progressively shorter, but this effect appeared to diminish when the silages were supplemented with concentrates. Data from one trial indicate that reducing the length of hay had no effect on voluntary intake when concentrates were fed.