ISSN:
1550-7408
Quelle:
Blackwell Publishing Journal Backfiles 1879-2005
Thema:
Biologie
Notizen:
Patterns for free and protein amino acids and related substances were determined for Tetrahymena limacis and 7 strains of T. pyriformis from axenic stationary phase cultures grown at 25° C by means of 2-dimensional chromatography in a butanol-acetic acid and phenol solvent system with ninhydrin and other polychromatic indicators. A uniform protein amino acid (PAA) pattern was observed in all strains. There were 14 color spots indicating 19 amino acids (including cysteic acid), identified as follows: alanine, arginine, aspartic acid, cysteine/cystine, cysteic acid, glutamic acid, glycine, leucine/isoleucine/phenylalanine, lysine/histidine, proline, serine, threonine, tyrosine, valine/methionine.The following free amino acids and related substances (FAAs) were identified with 14 spots (several different from these for PAA patterns) found in all strains: alanine, arginine, asparagine, aspartic acid, glutamic acid, glutamine, glycine, leucine/isoleucine/phenylalanine, lysine histidine. proline, serine, threonine, tyrosine, valine/methionine. T. limacis and strains LI and Gf-J of T. pyriformis exhibited these only. Chromatograms of 5 strains of T. pyriformis (PR. F. L3 WH52, HS), however, also contained 1 to 4 spots representing certain of the following substances: Cysteic acid, cysteine/cystine, taurine, and the unknowns X1, X2, and X3, having Rf's of 0.33, 0.79, and 0.72 respectively in 4:1 phenol-H2O system Excepting for F and L3, which were similar, the T. pyriformis strains showed quite different distributional patterns of these substances at 25°C. Other deviations in the distribution of the 6 compounds were noted in the chromatograms of 10° and 35° cultures of WH52 and HS. These findings on FAAs and PAAs are tabulated, along with those of previous investigators, to furnish comparisons on 13 strains of Tetrahymena pyriformis, T. limacis and 9 other species of protozoa.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1111/j.1550-7408.1961.tb01202.x
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