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  • Brassica napus  (4)
  • transgenic plants  (3)
  • Springer  (7)
  • American Chemical Society
  • American Meteorological Society
  • Cambridge University Press
  • 1995-1999
  • 1990-1994
  • 1955-1959  (7)
  • 1966
  • 1955  (7)
Sammlung
Schlagwörter
Verlag/Herausgeber
  • Springer  (7)
  • American Chemical Society
  • American Meteorological Society
  • Cambridge University Press
Erscheinungszeitraum
  • 1995-1999
  • 1990-1994
  • 1955-1959  (7)
Jahr
  • 1
    Digitale Medien
    Digitale Medien
    Synergistic activity of chitinases and β-1,3-glucanases enhances fungal resistance in transgenic tomato plants (1955)
    Springer
    Euphytica 85 (1955), S. 173-180 
    Details
    ISSN: 1573-5060
    Schlagwort(e): Lycopersicon esculentum ; tomato ; endochitinases ; β-1,3-endoglucanases ; fungal resistance ; transgenic plants
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Summary Simultaneous expression of a tobacco class I chitinase and a class I β-1,3-glucanase gene in tomato resulted in increased fungal resistance, whereas transgenic tomato plants expressing either one of these genes were not protected against fungal infection. After infection with Fusarium oxysporum f.sp. lycopersici, a 36% to 58% reduction in disease severity was observed in resistant tomato lines. Two transgenic lines largely recovered from the initial infection by the time wild-type tomato plants had died. The overall results are consistent with the observation that class I chitinases and class I β-1,3-glucanases synergistically inhibit the growth of fungi in vitro and provide the first experimental support to the hypothesis that such synergy can contribute to enhanced fungal resistance in planta.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00023946
    Standort Signatur Erwartet Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    A study of different (CaMV 35S and mas) promoter activities and risk assessment of field use in transgenic rapeseed plants (1955)
    Springer
    Euphytica 85 (1955), S. 411-416 
    Details
    ISSN: 1573-5060
    Schlagwort(e): Agrobacterium ; Brassica napus ; CaMV 35S promoter ; mas promoter ; gene expression ; risk assessment
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Summary Gene fusions between the β-glucuronidase (GUS) reporter gene and the promoters of the cauliflower mosaic virus 35S RNA transcript (CaMV 35S) and the mannopine synthase (mas) genes were introduced into rapeseed varieties via Agrobacterium-mediated transformation. Fluorometric assay of β-glucuronidase activity indicated different expression patterns for the two promoters. In seedlings, the CaMV 35S promoter had maximum activity in the primary roots, while the mas promoter was most active in the cotyledons. Etiolated seedlings cultured in the dark showed reduced activity of the mas promoter. Before vernalization at the rosette stage, both promoters were more active in older plant parts than in younger ones. At this stage the highest activity was recorded in cotyledons. After the plants had bolted reduced promoter function was detected in the upper parts of the transformed plants. Both promoters were found to be functional in the majority of the studied organs of transgenic rapeseed plants, but the promoter activity varied considerably between the organs at different developmental stages. The ability of pollen to transfer the introduced genes to other varieties and related species (e.g. Brassica napus and Diplotaxus muralis) by cross-pollination was studied in greenhouse experiments, and field trials were carried out to estimate the distance for biologically-relevant gene dispersal. In artificial crossing, the introduced marker gene was transferable into other varieties of Brassica napus. In field trials, at a distance of 1 metre from the source of transgenic plants, the frequency of an outcrossing event was relatively high (10-3). Resistant individuals were found at 16 and 32 metres from the transgenic pollen donors, but the frequency of an outcrossing event dropped to 10-5.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00023974
    Standort Signatur Erwartet Verfügbarkeit
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  • 3
    Digitale Medien
    Digitale Medien
    Transfer of disease resistance within the genus Brassica through asymmetric somatic hybridization (1955)
    Springer
    Euphytica 85 (1955), S. 247-253 
    Details
    ISSN: 1573-5060
    Schlagwort(e): asymmetric somatic hybridization ; Brassica napus ; Brassica nigra ; disease resistance transfer ; dot blot analysis
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Summary Asymmetric somatic hybrid plants between Brassica napus L. (oilseed rape genome AACC) and a transgenic line of Brassica nigra L. Koch (black mustard genome BB) were tested for their resistance against rapeseed pathogens Phoma lingam (black leg disease) and Plasmodiophora brassicae (club root disease). The transgenic B. nigra line used (hygromycin-resistant, donor) is highly resistant to both fungi, whereas B. napus (recipient) is highly susceptible. The asymmetric somatic hybrids were produced using the donor-recipient fusion method (with X-irradiation of donor protoplasts) reported by Zelcer et al. (1978) for the production of cybrids. Using hygromycin-B for selection, a total of 332 hybrid calli were obtained. Regenerants, resistant or susceptible to both diseases, were selected. Many hybrids expressed resistance to only one pathogen. Dot blot experiments showed that the asymmetric hybrid plants contained varying amounts of the donor genomic DNA. Furthermore, a correlation was detected between the radiation dose and the degree of donor DNA elimination.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00023953
    Standort Signatur Erwartet Verfügbarkeit
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  • 4
    Digitale Medien
    Digitale Medien
    The release of transgenic plants from containment, and the move towards their widespread use in agriculture (1955)
    Springer
    Euphytica 85 (1955), S. 425-431 
    Details
    ISSN: 1573-5060
    Schlagwort(e): consumer ; food ; genetic modification ; transgenic plants ; risk assessment
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Summary The use of transgenic plants in breeding makes it possible to utilise a wide variety of novel genes from unrelated plants, microbes and animals. Because of the diversity of genes that have now become available for modifying crop plants, it is agreed internationally that there should be a risk assessment before transgenic plants are grown outside the laboratory or glasshouse. Various aspects are considered in a risk assessment including any non-target effects of the transgene, changes in plant persistence and invasiveness, and the possibility of movement of the transgenes to wild populations by cross pollination. It is generally argued that the need for risk assessment and regulation should be determined by an analysis of certain products of transformation, rather than a risk assessment being required for all plants modified by the process of transformation. A possible consequence of considering the product only, however, could be that some of the products of conventional breeding may need to be assessed by the risk assessment procedures developed for transgenic plants. There are discussions with interest groups on the use of transgenic plants in the environment and in food products. It is likely that some form of labelling will be required for certain foods containing ethically-sensitive genes. There is little doubt that transgenic plants will make a significant contribution to agriculture in the coming decades. Developments in the patenting of genes, release regulations, food labelling, consumer reaction etc., will influence the rate of progress and should be considered in the strategic planning of plant breeding programmes.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00023976
    Standort Signatur Erwartet Verfügbarkeit
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  • 5
    Digitale Medien
    Digitale Medien
    Degradation of oxalic acid by transgenic oilseed rape plants expressing oxalate oxidase (1955)
    Springer
    Euphytica 85 (1955), S. 169-172 
    Details
    ISSN: 1573-5060
    Schlagwort(e): Brassica napus ; disease tolerance ; oxalic acid ; oxalate oxidase ; Sclerotinia sclerotiorum ; transformation
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Summary Oxalic acid is thought to have a primary role in the pathogenicity of several plant pathogens, notably Sclerotinia selerotiorum. A gene coding for the enzyme oxalate oxidase was isolated from barley roots and introduced into oilseed rape as a means of degrading oxalic acid in vivo. This report describes the production of several transgenic plants of oilseed rape and the characterisation of these plants by Southern, Western and enzyme activity assays. Plants were shown to contain an active oxalate oxidase enzyme and were tolerant of exogenously supplied oxalic acid.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00023945
    Standort Signatur Erwartet Verfügbarkeit
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  • 6
    Digitale Medien
    Digitale Medien
    Modification of oilseed rape to produce oils for industrial use by means of applied tissue culture methodology (1955)
    Springer
    Euphytica 85 (1955), S. 323-327 
    Details
    ISSN: 1573-5060
    Schlagwort(e): Brassica napus ; fatty acids ; gas chromatography ; Lunaria annua ; protoplast regeneration ; somaclonal variation
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Summary A programme of research was designed to investigate methods for the modification of the fatty acid profiles of high performance lines of oilseed rape (Brassica napus L.) in an attempt to produce lines with enhanced levels of industrially useful fatty acids. The methodology employed to achieve these objectives was based on the exploitation of somaclonal or protoclonal variation, and targeted somatic hybridization using wild cruciferous germplasm as fusion partners. A range of somaclonal lines was produced from shoot regeneration protocols. These lines underwent replicated, randomised glasshouse trials for morphological assessment followed by gas chromatographic analysis to monitor any changes in fatty acid profile. It was found that a small number of lines exhibited potentially useful changes in oleic acid and polyunsaturated fatty acid content. Protoplast regeneration and electrofusion protocols for a range of winter oilseed rape lines were developed, and methods for the isolation and fusion of protoplasts of the wild crucifer Lunaria annua (chosen for its high nervonic acid content) established.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00023962
    Standort Signatur Erwartet Verfügbarkeit
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  • 7
    Digitale Medien
    Digitale Medien
    Strategies for variety-independent genetic transformation of important cereals, legumes and woody species utilizing particle bombardment (1955)
    Springer
    Euphytica 85 (1955), S. 13-27 
    Details
    ISSN: 1573-5060
    Schlagwort(e): gene transfer ; crop species ; particle bombardment ; transgenic plants ; cereals ; legumes ; woody plants
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Summary The limiting component in the creation of transgenic crops has been the lack of effective means to introduce foreign genes into elite germplasm. However, the development of novel direct DNA transfer methodology, by-passing limitations imposed by Agrobacterium-host specificity and cell culture constraints, has allowed the engineering of almost all major crops, including formerly recalcitrant cereals, legumes and woody species. The creation of transgenic rice, wheat, maize, barley, oat, soybean, phaseolus, peanut, poplar, spruce, cotton and others, in an efficient and in some cases, variety-independent fashion, is a significant step towards the routine application of recombinant DNA methodology to the improvement of most important agronomic crops. In this review we will focus on key elements and advantages of particle bombardment technology in order to evaluate its impact on the accelerated commercialization of products based on agricultural biotechnology and its utility in studying basic plant developmental processes and function through transgenesis. Fundamental differences between conventional gene transfer methods, utilizing Agrobacterium vectors or protoplast/suspension cultures, and particle bombardment will be discussed in depth.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00023926
    Standort Signatur Erwartet Verfügbarkeit
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