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  • Articles  (8,476)
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  • Articles  (8,476)
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  • 1995-1999  (7,893)
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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    European journal of nutrition 38 (1999), S. 133-142 
    ISSN: 1436-6215
    Keywords: Key words Flavonoids – cancer-cell lines – proliferation –, cytotoxicity – apoptosis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Medicine
    Notes: Summary Flavonoids are polyphenolic compounds that occur ubiquitously in foods of plant origin. Their proposed protective role in tumor development may prevail especially in the intestinal tract due to direct exposure of intestinal epithelia to these dietary ingredients. We have screened more than 30 flavonoids for their effects on cell proliferation and potential cytotoxicity in the human colon cancer cell lines Caco-2, displaying features of small intestinal epithelial cells, and HT-29, resembling colonic crypt cells. In addition, for selected compounds we assessed whether they induce apoptosis by determining caspase-3 activation. Studies on the dose dependent effects of the flavonoids showed antiproliferative activity of all compounds with EC50 values ranging between 39.7 ± 2.3 μM (baicalein) and 203.6 ± 15.5 μM (diosmin). In almost all cases, growth inhibition by the flavonoids occured in the absence of cytotoxicity. There was no obvious structure-activity relationship in the antiproliferative effects either on basis of the subclasses (i.e., isoflavones, flavones, flavonols, flavanones) or with respect to kind or position of substituents within a class. In a subset of experiments we examined the antiproliferative activities of the most potent compound of each flavonoid subgroup in addition in LLC-PK1, a renal tubular cell line, and the human breast cancer cell line MCF-7. Out of four flavonols tested, three displayed almost equal antiproliferative activities in all cell lines but fisetin was less potent in MCF-7 cells. The flavanones bavachinin and flavanone inhibited growth of Caco-2 and HT-29 cells with lower EC50 values than that obtained in LLC-PK1 and MCF-7 cells. The lower susceptibility of LLC-PK1 and MCF-7 cells towards growth arrest was even more pronounced in the case of the flavone baicalein. Half maximal growth-inhibition in LLC-PK1 and MCF-7 required 2.5 and 6.6 fold higher concentrations than that needed in the intestinal cell lines. The flavonoids failed to affect apoptosis in LLC-PK1 and MCF-7, whereas baicalein and myricetin were able to induce apoptosis in HT-29 and Caco-2 cells. In conclusion, flavonoids of the flavone, flavonol, flavanone, and isoflavone classes possess antiproliferative effects in different cancer cell lines. The capability of flavonoids for growth inhibition and induction of apoptosis can not be predicted on the basis of their chemical composition and structure.
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  • 2
    ISSN: 1436-6215
    Keywords: Key words Dietary fat – adipose tissue – lipolysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Medicine
    Notes: Abstract Variations in total energy intake and composition of daily food play an important role in the regulation of metabolic processes and so, in the control of body weight. This study was designed in order to investigate the effect of a high-fat diet on lipolysis in isolated adipocytes. For this purpose, fourteen Wistar rats were divided into two groups and fed either a standard-fat diet or a high-fat diet ad libitum for 7 weeks. Adipocytes were prepared from fat pads by collagenase digestion and incubated in vitro in the absence or presence of various lipolytic agents. Lipolysis was measured by the release of glycerol into the medium during 90 min of incubation. We observed that a high amount of fat in the diet induced an enlargement of adipose tissue, which was accompanied by a reduction of β-adrenergic agonist-induced lipolysis, that could be due to a loss of β1 and β3-adrenoceptor number or to alterations of their coupling to adenylate-cyclase through the guanine nucleotide regulatory protein. New data about regional differences were provided by comparing two adipose locations (subcutaneous and visceral).
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  • 3
    ISSN: 1436-6215
    Keywords: Key words Wine polyphenols – 8-hydroxy-2'-deoxyguanosine – oxidative damage – 2-nitropropane – 1,2-dimethylhydrazine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Medicine
    Notes: Summary Background: Flavonoids are polyphenolic antioxidants occuring in vegetables and fruits as well as beverages such as tea and wine which have been thought to influence oxidative damage. Aim of the study: We wanted to verify whether a complex mixture of wine tannins (wine complex polyphenols and tannins, WCPT) prevent chemically-induced oxidative DNA damage in vivo. Methods: Oxidative DNA damage was evaluated by measuring the ratio of 8-hydroxy-2'-deoxyguanosine (80HdG)/ 2-deoxyguanosine (2dG) × 10−6 in hydrolyzed DNA using HPLC coupled with electrochemical and UV detectors. Results: We treated rats with WCPT (57 mg/kg p.o.) for 14 d, a dose 10-fold higher than what a moderate wine drinker would be exposed to. WCPT administration significantly reduced the ratio of 80HdG/2dG × 10−6 in liver DNA obtained from rats treated with 2-nitropropane (2NP) relative to controls administered 2NP only (33.3 ± 2.5 vs. 44.9 ± 3.2 × 10−6 2dG; μ± SE; p〈0.05). On the contrary, pretreatment with WCPT for 10 d did not protect the colon mucosa from oxidative DNA damage induced by 1,2-dimethylhydrazine (DMH). 2NP and DMH are hepatic and colon carcinogens, respectively, capable of inducing oxidative DNA damage. Conclusions: WCPT have protective action against some types of chemically-induced oxidative DNA damage in vivo.
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  • 4
    ISSN: 1436-6215
    Keywords: Key words Anthocyanidins – anthocyanins – comet assay – antioxidative potential – oxidized DNA-bases
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Medicine
    Notes: Summary Anthocyanins are common colored plant flavonoids, occurring as glycosides of the respective anthocyanidin-chromophores. Like other flavonoids, anthocyanidins are also expected to have antioxidative and antimutagenic properties in vivo, although only few data are available. To gain more knowledge on possible protective mechanisms in mammalian cells, we have compared their extracellular and intracellular antioxidative potential in vitro and in human colon tumor cells. We used Aronia melanocarpa Elliot anthocyanin (AA) concentrates, fractions thereof, concentrates from Elderberry, Macqui, and Tintorera fruits, as well as pure compounds. In vitro, antioxidative properties of the samples were studied with the ferric reducing ability assay (FRA assay). As a measure of intracellular oxidative/antioxidative effects, H2O2-induced strand breaks as well as oxidized DNA bases were determined in human tumor HT29 clone 19A cells using a microgelelectrophoresis assay (comet test). Major results were that isolated compounds (aglycons and glycosides) and complex plant samples are powerful antioxidants in vitro. In fact their activities by far exceeded those of Trolox and vitamin C in the FRA assay. Also H2O2-induced DNA strand breaks were reduced in cells treated with the complex plant extracts. In contrast, endogenous generation of oxidized DNA bases was not prevented. In summary, the intracellular steady state of oxidized DNA bases is not altered by anthocyanins or anthocyanidins. This findings raises questions with respect to the cancer preventive potential of anthocyanidins within specific tissues, such as the colon. Extracellularly, however, the compounds are potent antioxidants. This points to their potential for providing systemic protection in vivo, e.g., by scavenging oxidants in the blood stream and in the colon. Notably, both aglycons and glycosides have equally strong antioxidant activity.
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  • 5
    ISSN: 1436-6215
    Keywords: Key words Estrone – estrogen – obesity – dietary estrogen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Medicine
    Notes: Summary Background Estrone is a relatively abundant hormone widely distributed in tissues of animal and plant origin. It is a mild estrogen that induces increases in body weigt in experimental animals. The relative abundance of estrone esters in animal tissues suggests that it may also be found in foods, from which it may alter the mechanisms of body weight control. Aim of the study To measure the total estrone content in food and to determine whether this may affect body weight. Methods In the first part of the study, a method was devised for the measurement of total estrone content in food. This was applied to the analysis of estrone content in a variety of food. Finally, hyperlipidic diets (18.6 MJ/kg) with a total estrone content 0.89 ± 0.21 μmol/kg (control group) and 1.37 ± 0.13 μmol/kg (laced with estrone fatty esters) were given to rats during 15 days, in order to determine the influence of dietary estrone on the body mass. Zucker lean (Fa/?) rats weighting initially 200–215 g were used. The total estrone (essentially as fatty esters) content of food was investigated by combining a dried methanol extraction with saponification and measurement of the free estrone evolved through radioimmunoassay. Result The content of estrone was zero in some vegetables, but significant in fruits, meats, and especially fats, both of plant and animal origin. The application of these analyses to a standard recommended diet for humans may result in intakes of more than 1 μmol of estrone per day, a figure comparable to the estrogen production by women. When rats were exposed to a raised estrone content in a fat-rich diet, they significantly increased their body weights, doubling their rate off growth (1.99 g/day) compared with controls (0.81 g/day), but maintaining their plasma composition and the proportions of lipid, water, and protein in their carcasses. Conclusion The widely distributed estrone esters in food and their relatively high concentrations may result in high free hormone intakes in humans. The continued and massive intake of estrone may enhance tissue deposition and lead to obesity.
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    European journal of nutrition 38 (1999), S. 295-296 
    ISSN: 1436-6215
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Medicine
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    European journal of nutrition 38 (1999), S. 286-292 
    ISSN: 1436-6215
    Keywords: Key words Plant oils – tocopherols – sister chromatid exchanges
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Medicine
    Notes: Summary Background: Plant fats and oils are major sources of mono- and polyunsaturated fatty acids as well as vitamin E, the major fat-soluble antioxidants in human nutrition. Dietary antioxidants are expected to reduce cancer risk by minimizing DNA damage. Aim of the study: To compare the effects of γ-tocopherol rich corn oil and the mixture of the α-tocopherol rich olive/sunflower oil on plasma concentration of tocopherols and on the frequency of sister chromatid exchange (SCE), an indicator of DNA damage. Methods: This study had a double-blind, cross-over design and was conducted in 20 normal healthy non-smoking males aged 19–31 years. Design included a 2-week adjustment period and two 2-week test periods in which diets containing 30% energy as fat including either 80 g of corn oil (CO) (20 mg α-tocopherol, 100 mg γtocopherol) or 80 g of olive/sunflower oil (OSO) (24 mg α-tocopherol, 2.4 mg γ-tocopherol) as the main fat-source, were given. Blood samples for analysis of SCE rate and content of tocopherols were collected at the beginning (T0), after adjustment (T1) and after the test period (T2) in intervals of 2 weeks. Results: After two weeks of the corn oil diet, the plasma concentration of γ-tocopherol increased but α-tocopherol decreased significantly compared to the olive/sunflower oil diet. The concentration of α-tocopherol increased (CO: 22.99 ± 1.11 vs. OSO: 24.40 ± 1.49 μmol/l) and that of γ-tocopherol decreased (CO: 4.19 ± 0.29 vs. OSO: 2.99 ± 0.25 μmol/l) after the olive/sunflower oil diet. Intake of the corn oil diet was associated with reduced SCE rate and intensity, whereas there was no change in SCE after the olive/sunflower oil diet (CO: 7.66 ± 0.25 vs. OSO: 8.06 ± 0.47 mean SCE/cell). Conclusions: The combination of γ-tocopherol with α-tocopherol in corn oil diet despite the lower α-tocopherol equivalents/diene acid equivalents ratio achieved better protection against DNA damage than α-tocopherol alone in the olive/sunflower oil diet.
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    European journal of nutrition 38 (1999), S. 1-13 
    ISSN: 1436-6215
    Keywords: Key words Pharmacotherapy – obesity – appetite suppressant – thermogenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Medicine
    Notes: Summary Many new substances are currently being investigated for their usefulness in the pharmacotherapy of obesity. Most drugs interfere with monoamine neurotransmitter (serotonin, noradrenalin, dopamine and histamine) effects and act as an appetite suppressant. Other approaches are to primarily increase thermogenesis (e.g. β3-adrenoceptor agonists), or to decrease fat absorption by inhibiting the pancreatic lipase (orlistat). New promising agents are substances that increase the effect of corticotropin releasing factor (CRF) or urocortin in the brain (CRF-binding protein ligand inhibitor) and a neuropeptide Y (NPY) Y5 receptor antagonist. The clinical relevance of leptin in the therapy of obesity is probably limited, but can not be fully evaluated at the moment. As obesity has a multifactorial basis, all these substances have in common the fact that they can not cure obesity. They should only be used as an adjunct to classical strategies like diet and exercise in severe obesity. For developing new, perhaps even more specific pharmacological agents, further research is needed to understand the individually different genetic and physiological basis of obesity.
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    European journal of nutrition 38 (1999), S. 35-44 
    ISSN: 1436-6215
    Keywords: Key words Carotenoids – lycopene – lutein –α-carotene –β-carotene – oxidation – antioxidant – human
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Medicine
    Notes: Summary Aim of the study: The present study was conducted to investigate changes in the plasma concentration of carotenoids and carotenoid oxidation products, vitamin A, α- and γ-tocopherol, and ubiquinone-10 during a dietary intervention trial with 23 male healthy volunteers. Method: A two week carotenoid depletion period was followed by a daily consumption of 330 mL tomato juice (40 mg lycopene), then by 330 mL carrot juice (15.7 mg α-carotene and 22.3 mg β-carotene), and then by a 10 g spinach powder preparation (11.3 mg lutein and 3.1 mg β-carotene) served with main meals for two weeks, respectively. Blood samples were collected in the morning after an overnight fasting and carotenoids, vitamin A, tocopherols, and ubichinone were analyzed by reversed-phase HPLC. Results: During the tomato juice intervention, plasma concentrations of trans- and cis-lycopene increased 2-fold compared to the depletion period. Lycopene oxidation products could be demonstrated in plasma and were significantly elevated compared to control (p〈0.001). After two weeks of carrot juice consumption, α-carotene and β-carotene concentrations increased 8.6- and 3.2-fold, respectively. Finally, during the spinach consumption period the lutein concentration increased 2-fold, while the β-carotene concentrations were still elevated 2-fold. Conclusions: The moderate change in dietary habits, e.g., the consumption of 330 mL of carotenoid-rich vegetable juices caused significant changes in the plasma carotenoid concentrations, indicating a high bioavailability of carotenoids from the processed vegetable products. The changes in plasma carotenoid concentrations reflected the carotenoid composition of the consumed foods. However, particularly during the tomato juice intervention period the occurrence of lycopene oxidation products and cis-lycopene isomers in plasma was eminent. The formation may be due to antioxidant reactions of lycopene in the organism.
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  • 10
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    European journal of nutrition 38 (1999), S. 51-75 
    ISSN: 1436-6215
    Keywords: Key words Iron – bioavailability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Medicine
    Notes: Summary In this review a broad overview of historical and current methods for the assessment of iron bioavailability was given. These methods can be divided into iron solubility studies, iron absorption studies, endpoint measures, and arithmetic models. The pros and cons of all methods were discussed. First, studies on in vitro and in vivo iron solubility have been described. The disadvantages of iron solubility include the impossibility of measuring absorption or incorporation of iron. Furthermore, only the solubility of nonheme iron, and not heme iron, can be studied. Second, we focused on iron absorption studies (either with the use of native iron, radioiron or stable iron isotopes), in which balance techniques, whole-body counting or postabsorption plasma iron measurements can be applied. In vitro determination of iron absorption using intestinal loops or cell lines, was also discussed in this part. As far as absorption studies using animals, duodenal loops, gut sacs or Caco-2 cells were concerned, the difficulty of extrapolating the results to the human situation seemed to be the major drawback. Chemical balance in man has been a good, but laborious and expensive, way to study iron absorption. Whole-body counting has the disadvantage of causing radiation exposure and it is based on a single meal. The measurement of plasma iron response did not seem to be of great value in determining nutritional iron bioavailability. The next part dealt with endpoint measures. According to the definition of iron bioavailability, these methods gave the best figure for it. In animals, the hemoglobin-repletion bioassay was most often used, whereas most studies in humans monitored the fate of radioisotopes or stable isotopes of iron in blood. Repletion bioassays using rats or other animals were of limited use because the accuracy of extrapolation to man is unknown. The use of the rat as a model for iron bioavailability seemed to be empirically based, and there were many reasons to consider the rat as an obsolete model in this respect. The double-isotope technique was probably the best predictor of iron bioavailability in humans. Disadvantages of this method are the single meal basis and the exposure to radiation (as far as radioisotopes were used). Finally, some arithmetic models were described. These models were based on data from iron bioavailability studies and could predict the bioavailability of iron from a meal.
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  • 11
    ISSN: 1436-6215
    Keywords: Key words Obesity – oleoyl-estrone – leptin – Zucker fa/fa rat – white adipose tissue
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Medicine
    Notes: Summary Background: Oleoyl-estrone elicits powerful slimming effects on lean and obese rats, sparing protein, lowering appetite and maintaining energy expenditure. Leptin synthesis is markedly reduced by oleoyl-estrone. However, this effect is not observed in the obese Zucker fa/fa rats; these rats do not fully respond to leptin but they lose fat under oleoyl-estrone treatment. Aim of the study: To determine the role of leptin in the conversion of estrone to fatty-acyl estrone in white adipose tissue both in vivo in Zucker lean and obese rats, and in vitro. Methods: Two series of experiments were performed: a) Growth and differentiation of 3T3L1 preadipocytes into adipocytes followed by incubation with tritium-labeled estrone in the medium in the presence / absence of 1 nM leptin, and estimation of the incorporation of label into estrone and estrone ester fractions of cell extracts. b) Zucker lean (Fa/?) [ZL] and obese (fa/fa) [ZO] rats were injected i.v. with carrier-free oleoyl-estrone in chylomicra-sized liposomes, then euthanized after 10 min. Free and esterified estrone were measured in blood, liver, muscle, skin, white adipose tissue (WAT), and brown adipose tissue(BAT). Results: In the first study, in a 72-h incubation, adipocytes took up 20-27% of the medium estrone. In the leptin(−) controls, 47% of the label in the cell fraction was in the form of estrone esters and 45% as free estrone; in the leptin (+) cells, 71% of the label was in the estrone ester fraction and 24% was free estrone. In the second study, a large part of the injected tritium-label remained in the ZO blood, with only a small part remaining in ZL. In ZL 39% of the label was found in the tissues in the form of free estrone, and in ZO only 22%; in both cases about half of it was in WAT. Plasma free estrone levels were 0.3±0.1 nM in ZL and 0.5±0.3 nM in ZO, and esterified estrone was 242±99 nM for ZL and 201±29 nM for ZO. Plasma leptin levels were 1.73±0.16 ng/ml in ZL and 61.0±1.4 ng/ml in ZO. Conclusion: The presence of an intact leptin pathway is critical for the uptake and synthesis of estrone esters as well as for the plasma acyl-estrone turnover. The presented results show a direct relationship between oleoyl-estrone and leptin in the WAT. A fully functional leptin pathway is needed for the synthesis of acyl-estrone and the removal of free estrone from the bloodstream, as well as for the disposal of excess circulating oleoyl-estrone. This has a direct bearing on human and animal obesity, since estrone induces increases in fat deposition.
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  • 12
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    European journal of nutrition 38 (1999), S. 143 
    ISSN: 1436-6215
    Keywords: Key words Phytoestrogen – cholesterol – DNA damage – comet assay – antioxidant
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Medicine
    Notes: Summary Background: Phytoestrogens are a major component of Asian diets and may be protective against certain hormone-dependent cancers (breast and prostate) and coronary heart disease. They may also have antioxidant function in scavenging potentially harmful free radicals and thus decreasing oxidative attack on DNA. Aims of the study: A pilot study to determine the effects of a phytoestrogen supplement, in the form of soy milk, on plasma LDL and HDL cholesterol levels and DNA damage in men. Methods: Ten healthy men participated in the study and were assigned to one of three groups consuming 1 litre of either soy milk, rice dream (vegetable protein control) or semi-skimmed cow's milk (animal protein control) each day for 4 weeks. Results: The soy supplement caused significant increases in plasma genistein and daidzein concentrations despite considerable interindividual variation (P〈0.001). Supplementation with soy resulted in a decrease in oxidative damage to DNA bases detected using the comet assay compared with controls (P〈0.05). However, there was no significant effect of the soy supplement on plasma cholesterol or triglyceride levels in comparison with control groups. Conclusions: A 4 week soy milk supplementation in healthy volunteers does not alter serum cholesterol levels but can have a protective effect against oxidative DNA damage in lymphocytes.
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  • 13
    ISSN: 1436-6215
    Keywords: Key words green tea – linoleic acid – antioxidants – lipid peroxidation – hemostasis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Medicine
    Notes: Summary Background: Green tea contains polyphenolic catechins which can act as antioxidants and thus decrease the risk for cardiovascular diseases. Aim of the study: To investigate whether green tea extract differs from placebo in its effects on markers of antioxidant status, lipid peroxidation, nitric oxide production, thromboxane production, and blood coagulation during a controlled high linoleic acid diet in healthy subjects. Methods: Twenty healthy non-smoking females (23–50 years) participated in a 4-week controlled intervention study. The experimental diet was rich in linoleic acid (9 en%) and contained fat, protein and carbohydrates: 27, 14, and 59 en%, respectively. In addition, the subjects ingested encapsulated green tea extract (3 g/d) or placebo mixture in a double-blind manner. Fasting blood samples and five 24-hour urines were collected before and at the end of the 4-week experimental period. Same samples were received from 10 control subjects. Results: Green tea extract significantly decreased plasma malondialdehyde (MDA) concentration in comparison with the placebo treatment. The treatments did not differ in serum lipids, indicators of antioxidant status, urinary 8-iso-prostaglandin F2α, 2,3-dinor-thromboxane B2, nitric oxide metabolites or coagulation indicators. Conclusions: We conclude that an amount of green tea extract which corresponds to 10 cups of tea per day for 4 weeks does not have specific effects on several indicators related to risk of cardiovascular diseases in comparison with placebo treatment. The relatively small but significant decrease in lipid peroxidation indicated by decreased plasma MDA was not associated with changes in markers of oxidative stress (urinary 8-iso-prostaglandin F2α and blood oxidized glutathione) or hemostasis.
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  • 14
    ISSN: 1436-6215
    Keywords: Key words Long chain polyunsaturated fatty acids – peroxidation – vitamin E – weanling rats
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Medicine
    Notes: Summary Background: Tissue 10:4(n-6) and 22:6(n-3) status have been correlated with neonatal development and growth. Artificial formulas for neonates have been supplemented with long chain polyunsaturated fatty acids (LCP) from animal and marine sources which may enhance sensitivity of cellular membranes to oxidative damage. Diet-derived antioxidants like vitamin E play a key role in the protection of tissue lipids against oxidation. Aim of the study: We seek to determine the influence of dietary vitamin E on tissue sensitivity to oxidative stress in rats fed for 4 weeks on diets enriched in (n-3) and (n-6) long-chain polyunsaturated fatty acids. Methods: Weanling rats received 10% fat diets that provided 18:1(n-9), 18:2(n-6) and 18:3(n-3) in a similar ratio to that of rat milk (group A), supplemented with fish oil (groups B and B+E) and supplemented with (n-6) and (n-3) LCP from an animal phospholipid concentrate (groups C and C+E). Vitamin E (500 mg vitamin E/kg fat) was added to diets B+E and C+E. Tissue fatty acid content and the activities of catalase, superoxide dismutase, glutathione transferase und glutathione peroxidase in liver and brain were measured. Glutathione status, vitamin E and the production of thiobarbituric acid reactive substances (TBARs) after incubation of erythrocyte, liver and brain lipids with inducers of enzymatic or non-enzymatic lipid peroxidation was measured. Results: Group B registered significantly lower total superoxide dismutase acitvity than group B+. Catalase activity was significantly higher in group C than in group C+E. Hepatic total and reduced glutathione levels were decreased in vitamin E supplemented groups compared to unsupplemented ones. TBARs production in erythrocyte lipids was significantly higher in groups B and C compared to vitamin E supplemented groups B+E and C+E. Conclusions: This study shows that the addition of vitamin E protected erythrocyte and liver microsome lipids enriched in (n-3) and (n-6) LCP from lipid peroxidation during the postnatal development of rats. The protection was more effectively in group C+E than in group B+E.
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  • 15
    ISSN: 1436-6215
    Keywords: Key words Children – adolescents – nutrition counselling – dietary evaluation – scores
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Medicine
    Notes: Summary An intervention study by documented dietary counselling was carried out in a sample of 9 children and adolescents (12–,15 years) living in a full-time institution in Dortmund. Three weighed dietary records were collected over 3–7 days, one before and one after each of two individual nutrition counselling sessions, which were based on the recommended intake of food groups defined by the Optimized Mixed Diet (OMD), a quantitative preventive dietary conception for children and adolescents. As univariate dietary parameters (e.g., cholesterol intake), which are often used to show the effectiveness of nutrition counselling, do not take into account the multivariate complexitiy of nutrition, we developed 3 multivariate scores to measure the effectiveness of nutrition counselling. The are definded as: Recommended Food group change Score (RFS): Average change in the amounts of the deviations (%) from the reference food groups values (OMD = 100 %) before and after counselling taking into account the aim (eat mor/less) of the counselling session, exclusively based on the food groups addressed during counselling. Total Food group change Score (TFS): Average change in the amounts of the deviations (%) from the reference food group values (OMD = 100 %) before and after counselling based on all food groups consumed. Nutrient Improvement Score (NIS): Average change in the negative deviations (%) of 8 vitamins and 8 minerals from the German reference values for nutrient intake (Deutsche Gesellschaft für Ernährung (DGE) = 100 %) before and after counselling. On average, the intakes of the food groups mentioned during the first counselling session improved considerably (RFS = +36 %), the change in the intake of all food groups was small (TFS = +6 %) and the nutrient intakes did not improve (NIS = 0 %). From the second counselling session the values of the RFS was +10 %, of the TFS was +6% and of the NIS +3 %. This means that the success of counselling on one dietary criterion does not guarantee success on others. Our food and nutrient based scores together with a detailed food intake assessment give an example of multivariate measurements of nutrition counselling outcomes.
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  • 16
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    European journal of nutrition 38 (1999), S. 218-226 
    ISSN: 1436-6215
    Keywords: Key words Sugar alcohol – lactitol – breath hydrogen – lactose malabsorption – gastrointestinal symptons – diarrhea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Medicine
    Notes: Summary The hydrogen breath analysis test was performed in healthy Thai adults to determine lactitol tolerance. The study was conducted in 39 individuals (11 males and 28 females) aged 18–41 years. All volunteers agreed to participate in this study after the risks and benefits had been fully explained. Subjects were requested not to consume milk, milk products, or high-vegetable diets for a day and to fast from 10 p.m. of the day preceding the test day. After consumption on the test diet (12 and 20 g of lactose or lactitol, respectively, in 250 mL water), the subjects recorded the severity of symptoms for 24 hours. Breath samples were collected after fasting and after consumption of the test diet at 30 min intervals over the 7-hour study period. Breath samples were analyzed for hydrogen using gag chromatography. After consumption of 12 g lactose, the prevalence of lactose malabsorbers was established. The increment of a peak breath hydrogen level of ≥ 20 ppm above the baseline level was used as an indicator of lactose malabsorption. The lactose malabsorbers were further classified as lactose tolerants or lactose intolerants according to the gastrointestinal symptoms observed. All 39 healthy Thai adults could be classified into 3 groups as follows: 9 (23%) lactose absorbers (LA), 15 (38.5%) lactose malabsorber/tolerants (LMT) and 15 (38.5%) lactose malabsorber/intolerants (LMI). Using the hydrogen breath test, 67% of the subjects were identified as lactitol intolerants after the consumption of 12 g lactitol. The lactitol intolerants comprised 53.8% of LMI, 34.6% of LMT, and 11.5% of LA. Among all subjects, one third of LA (33%), two thirds of LMT (60%), and 93% of LMI were lactitol intolerant. In addition, gastrointestinal symptoms such as flatulence and abdominal pain were most pronounced in LMI. Diarrhea was also a prominent manifestation after consumption of 12 g lactitol. Therefore, it was finally decided that 20 g lactose or lactitol were not given to LMI because of the risk of gastrointestinal symptoms. After high doses (20 g) of lactose and lactitol consumption, most LMT developed more symptoms than did LA and the main symptom was diarrhea. Consumption of 20 g lactose resulted in fewer symptoms than 20 g lactitol in both LA and LMT. On the basis of the hydrogen breath test, most LA tolerated 12 g lactitol without gastrointestinal symptoms except some flatulence whereas most LMT and LMI did not. Twenty g lactitol was not tolerated by both LA and LMT because there was diarrhea among the subjects, especially in LMT. Although the hydrogen breath analysis test is the best method for identification of lactose malabsorption, it is not the best method to identify lactitol intolerance. A hydrogen concentration of 15 ppm above the baseline level was found to be the best cut-off point to indicate lactitol intolerance although sensitivity was 85% and specificity only 38% in this study. It was further concluded that there is a greater susceptibility to lactitol in human lactose malabsorbers than in lactose absorbers. Our findings might be relevant for the limited use of lactitol in Thailand.
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  • 17
    ISSN: 1436-6215
    Keywords: Key words Ergogenics – amino acids – exercise – arginine aspartate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Medicine
    Notes: Summary Background: Athletes consume arginine and/or aspartate as potential nutritional ergogenics. Their metabolic effects are controversial and there is some evidence that ingestion of large doses of single amino acids can adversely affect the nitrogen balance or induce an amino acid imbalance. Nevertheless, the general metabolic influence of an arginine aspartate supplementation during a prolonged exercise bout has not yet been investigated. Aim of the study: The aim of this study was, therefore, to investigate the general metabolic impact of a chronic supplementation with arginine aspartate in endurance-trained athletes at rest and during a marathon run. Methods: Fourteen endurance-trained runners participated in this field study which was carried out according to a double-blind crossover design. 15 g of arginine aspartate or a carbohydrate-based placebo were supplemented daily for 14 days before a marathon run. Blood samples for analysis of metabolites and hormones were collected shortly before the run, after 31 km, at the end of the run, and after a recovery period of two hours. Additionally, the respiratory exchange ratio was determined during the run. Results: The plasma level of carbohydrate (glucose, lactate, pyruvate) and fat metabolites (fatty acids, glycerol, β-hydroxybutyrate), cortisol, insulin, ammonia, lactate dehydrogenase, and creatine kinase as well as the respiratory exchange ratio were unaffected by the supplementation. In contrast, the plasma level of somatotropic hormone, glucagon, urea, and arginine were significantly increased, and the level of most of the remaining plasma amino acids as well as their sun was significantly reduced. Conclusions: There was no obvious metabolic benefit derived from the chronic supplementation with arginine aspartate. And since furthermore the consequences of a reduction of the total plasma amino acid level are not known, the practice of using single amino acid supplements as potential ergogenics should be critically reevaluated.
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  • 18
    ISSN: 1436-6215
    Keywords: Key words Conjugated linoleic acid – hormones – metabolites – lipoproteins – fatty acids – swine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Medicine
    Notes: Summary Background: Conjugated linoleic acids (CLA)# refer to a group of linoleic acid (18:2)-derived isomers with conjugated double bonds mostly at carbon atoms 9 and 11 or 10 and 12, and with all possible cis and trans combinations. CLA is a newly recognized nutrient that functions to regulate energy retention and metabolism and that causes a serum lipoprotein profile considered to be less atherogenic. However, rodent models that have been frequently used for these studies are only of limited use because of distinct differences in physiology, compared with man. Additionally, possible differences in food intake between the experimental groups remained often unconsidered in those studies. Thus, it can not be excluded that the beneficial effects of CLA reported in a series of studies may be due, at least partially, to differences in nutrient and energy ingested. Aim of the study: This prompted us to undertake an investigation on the action of CLA by using a pig model and a feeding regimen with controlled amounts of food and antioxidants. The parameters used to assess CLA-specific action were selected hormones and metabolites involved in energy metabolism, individual lipoproteins and the appearance of CLA in fasting serum and erythrocyte membranes. Blood as an easily available biological sample was used for investigation. Methods: For that purpose 16 adult female pigs were divided into two groups of 8 each, and were isoenergetically fed diets containing 0 (control diet) or 1% level of CLA (by weight) for 6 weeks. Plasma concentrations of total and free thyroxine (T4), total and free triiodothyronine (T3), and insulin were measured by radioimmunoassays. Plasma non-esterified fatty acids (NEFA), β-hydroxybutyrate, circulating blood ATP and other clinical chemical variables were determined using enzymatic assays. The concentration of α-tocopherol was determined by high perfomrance liquid chromatography. The lipoproteins VLDL (density 〈1.019 kg/L), LDL (density 1.019 – 1.063 kg/L), and HDL (density 〉1.063 kg/L) were isolated by step-wise ultracentrifugation. Fatty acids of the dietary oils, serum and blood cell membranes were separated and quantified by gas chromatography. Results: At week 6, body weights of the pigs fed the CLA-supplemented diet were not different from that of the controls. CLA-treated pigs exhibited a 37% higher concentration of fasting serum insulin than their controls receiving no CLA (P = 0.11). Circulating free and total T4 and T3 as well as serum levels of β-hydroxybutyrate, α-tocopherol, protein, glucose, urea, creatinine and circulating blood ATP remained unaffected by CLA supplementation. Serum concentrations of non-esterified fatty acids were reduced by 38% in CLA-treated pigs relative to the controls, although this difference was not significant. CLA-treated pigs tended to have lower leukocyte counts in blood than their controls (P 〈0.1). Erythrocyte and platelet counts, the hematocrit and hemoglobin concentration were similar between the groups. Serum of CLA-treated pigs showed a trend toward increased levels of triacylglycerols, cholesterol and phosphatidylcholine in the very low density and low density lipoproteins (LDL), without distinct changes in the high density lipoprotein fraction (HDL). The LDL cholesterol to HDL cholesterol ratio was significantly increased by CLA. When pigs were fed CLA at a dietary level of 1%, limited proportions of CLA appeared in fasting serum (1.6%) and erythrocyte membranes (1.1%). Conclusions: Under the present experimental conditions there appeared to be parallels between the effects of CLA and the reported effects of trans fatty acids in the mode of action on lipoproteins and insulin. The failure to demonstrate significant beneficial effects of CLA on the lipoprotein profile which have been observed in other studies requires further research.
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  • 19
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    Journal of geographical systems 1 (1999), S. 277-303 
    ISSN: 1435-5949
    Keywords: Key words: Higher education ; geodemographics ; participation rates ; JEL classification: C80 ; I21 ; I28 ; J11
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geography
    Notes: Abstract. Higher education in England has expanded rapidly in the last ten years with the result that currently more than 30% of young people go on to university. Expansion is likely to continue following the recommendations of a national committee of inquiry (the Dearing Committee). The participation rate is known to vary substantially among social groups and between geographical areas. In this paper the participation rate is calculated using a new measure, the Young Entrants Index (YEI), and the extent of variation by region, gender and residential neighbourhood type established. The Super Profiles geodemographic system is used to facilitate the latter. This is shown to be a powerful discriminator and to offer great potential as an alternative analytical approach to the conventional social class categories, based on parental occupation, that have formed the basis of most participation studies to date.
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  • 20
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    Journal of geographical systems 1 (1999), S. 305-321 
    ISSN: 1435-5949
    Keywords: Key words: GIS, urban systems model, land use – transportation model, traffic analysis zone ; JEL classification: R11, R14, R41
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geography
    Notes: Abstract. The main purpose of this paper is to explore a possible integration for the entire transportation modeling procedure – from data inventory to future demand forecasting – by implementing integrated land use and transportation models with a geographic information system (GIS). In order to make an integrated, procedural modeling system possible, Land Use and Transportation modeling system with GIS (LUTGIS) has been developed and presented in this paper.  There are four sub-systems in LUTGIS: (1) a data inventory system, (2) a traffic analysis zone generation system, (3) an integrated land use and transportation modeling system, and (4) a graphic user interface (GUI) system. Since the main target of this paper is to explore a possible way to create a viable system, LUTGIS integrates currently available and user-friendly computing technologies. For both transportation planners and administrative decision-makers, such an operable system is very desirable for sharing information so they may arrive at a consensus through the use of LUTGIS, an integrated land use and transportation modeling system.
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  • 21
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    Papers in regional science 78 (1999), S. 21-45 
    ISSN: 1435-5957
    Keywords: JEL classification: C52, R10, R12, R14, R39 ; Key words:New economic geography, spatial statistics, spatial modeling, methodology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geography , Economics
    Notes: Abstract. Krugman states that “Regional science is not a unified subject. It is best described as a collection of tools.” Unfortunately such a perspective fails to fully acknowledge theoretical dimensions of the accompanying refocusing on geographic expressions of economic linkages, such as those highlighted in spatial externalities specifications. Such promulgated aspects of the spatial economic landscape relate to map pattern, and certainly the spatial statistics and spatial econometrics theory that accompanies it, as well as the underlying substantive theory garnered from a variety of sources. The principal implication is other than “loose- jointed, do-the-best-you-can theorizing”.
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  • 22
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    Papers in regional science 78 (1999), S. 111-116 
    ISSN: 1435-5957
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    Topics: Geography , Economics
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  • 23
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    Papers in regional science 78 (1999), S. 117-118 
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    Topics: Geography , Economics
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  • 24
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    Papers in regional science 78 (1999), S. 157-177 
    ISSN: 1435-5957
    Keywords: JEL classification: R1, R12 ; Key words:Central place theory, choice rules, generalised Voronoi diagrams, market areas
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geography , Economics
    Notes: Abstract. Although a variety of modifications of classical central place theory has been proposed, one area that remains unexplored is the effect of relaxing the nearest centre assumption for the purchase of a specified basket of goods within a given hierarchical level. This article examines the effect of such a relaxation on central place market areas by using higher-order Voronoi diagrams. When used to model market areas, higher-order Voronoi diagrams can be interpreted as overlapping and probabilistic regions. These diagrams construct market areas based on the assumption that consumers choose from a set of $k (k = 1, 2, \ldots, n)$ nearest centres of the same hierarchical level. If consumers are assumed to be indifferent between the k centres, the appropriate market areas are given by the order-k Voronoi diagram. In this case, it is shown that sales potentials are consistent with those that result when the nearest centre assumption is in effect. If consumers are assumed to have a preference for nearer centres, market areas are defined by the ordered, order-k Voronoi diagram. This situation generates sales potentials which can vary between centres.
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  • 25
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    Lasers in medical science 14 (1999), S. 2-19 
    ISSN: 1435-604X
    Keywords: Keywords: Corneal topography; Fluorescence; Holography; Interferometry; Moiré; Partial coherence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Physics , Technology
    Notes: Abstract. Corneal topography has, due to developments in refractive surgery and contact lens fitting, become a widely used diagnostic tool. Many types of topographers have been introduced, but there is some confusion on classification and subsequent principal possibilities of the various devices offered to the practitioner. The purpose of the study reported here was to make an inventory of developed devices, analyse the basic principles and create a classification based on optical principles. A literature search was done using Medline, the IBM Patent Server, and references found in articles and patents. This search resulted in a variety of descriptions that could be classified into 12 groups according to their use of light source and light-matter interaction of which four groups have representatives on the commercial market. This classification can be used by researchers and practitioners to gain insight into the possibilities of a given device in relation to the desired application.
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  • 26
    ISSN: 1435-604X
    Keywords: Keywords: Interstitial laser coagulation; Laser applicator; Laser induced thermotherapy; Liver tumours; Nd-YAG laser
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Physics , Technology
    Notes: Abstract. A newly developed diffusing laser applicator was examined for interstitial laser coagulation (ILC) of liver tumours. The applicator consisted of a matted quartz core and a quartz glass dome, also matted on its inner surface and sealed to the fibre. The applicator provided a homogeneous light intensity distribution over an active length of about 20 mm. Lesions were created in an ex-vivo porcine liver model using a Nd-YAG laser comparing the new diffusing tip with a Ringmode®-ITT applicator in order to find optimal laser parameters and damage thresholds. The lesions were investigated using macroscopic size measurement, volume calculation and histological examination (H&E, NADPH-dehydrogenase). The damage threshold of the diffusing tip was 6 W at 14 min exposure time whereas the Ringmode®-ITT applicator had its limit at 5 W and 12 min exposure. Comparing various exposure times showed that treatment over a time of more than 840 s did not significantly increase the lesion volume. At 5 W and 720 s the mean lesion volume was 6.9±1.1 cm3 with the diffusing tip and 6.3±0.6 cm3 with the Ringmode®-ITT applicator, both having a slight ellipsoidal shape. Hence, the created lesions were not significantly different for both applicators when the same laser parameters were applied. On the other hand, the new diffusing tip had a higher damage threshold and was therefore capable of producing maximal coagulation volumes of up to 7.9±0.5 cm3 at 5 W and 20 min. The experiments showed that lesions with a dimension of 31×22 mm can be achieved with the diffusing applicator which seem suitably sized for treating small human liver metastases in a single laser session.
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  • 27
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    Lasers in medical science 14 (1999), S. 62-66 
    ISSN: 1435-604X
    Keywords: Keywords: Laser myringotomy; Otitis media with effusion; Tympanic membrane
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Physics , Technology
    Notes: Abstract. A prospective comparative study was done to assess the validity of laser myringotomy (L-myringotomy) when compared to the insertion of ventilating tubes (VT). In 23 children with chronic otitis media with effusion, a VT was inserted in the left ear while a laser myringotomy was performed in the right ear by using the CO2 Sharplan 1030 laser. After a six month follow-up conducted during the winter months, the results with the laser myringotomy were equal to those in the left ear with the VT. During the follow-up period, three ears required additional laser myringotomy either for early closure or because of otitis media and the accumulation of effusion in the middle ear cavity. Nevertheless, the use of laser has the advantage that there is no foreign body in the ear, the tympanic membrane is closed after four to seven weeks, and since the procedure is very short, the time for anaesthesia is minimal.
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  • 28
    ISSN: 1435-604X
    Keywords: Keywords: Chorioallantoic membrane (CAM); Liposomes; Methylene blue; Ovarian cancer; Photodynamic therapy; Photosensitiser
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Physics , Technology
    Notes: Abstract. The disappointing results with either surgery alone and/or chemotherapy in the treatment of malignant ovarian tumours have led to an increased interest in additional treatment schedules. Photodynamic therapy (PDT), a modality involving the use of a photosensitising drug and activating light, is being used increasingly as a local treatment for neoplastic lesions. The synthesis and evaluation of new photosensitisers for the treatment of gynaecological lesions and malignancies continues to be an active area of investigation for proper application of the photodynamic process in the gynaecological field. The effect of PDT using methylene blue (free and combined with liposomes) as a photosensitiser for treating human ovarian malignant tumours cultivated on the chorioallantoic membrane was evaluated. Two days after PDT, the treated implanted tumours were markedly decreased in size. Areas of necrosis with black coloration, dryness and eschar formation were observed. Five days after PDT, tumour remission was clearly observed in all the treated tumours. Photodynamic therapy using methylene blue (aqueous and coupled with liposomes) is effective for treating the ovarian malignancies and it will be capable of achieving complete eradication of visible tumours in patients with superficial lesions.
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  • 29
    ISSN: 1435-604X
    Keywords: Keywords: Aminolaevulinic acid; Dermatology; Photodynamic therapy; Photosensitisation; Protoporphyrin IX; Skin lesions
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Physics , Technology
    Notes: Abstract. The method of surface-detected fluorescence has been used to monitor the emission intensity from 5-aminolaevulinic acid (ALA)-induced protoporphyrin IX (PpIX) in lesions and corresponding adjacent normal skin. Three types of lesions were examined: psoriatic plaques, actinic keratosis and basal cell carcinoma. This study included a total of 14 human volunteers on whom ALA-induced PpIX formation and clearance was monitored for a total of 48 h post-ALA application. Both an ALA dose-ranging study, as well as a comparison of results between normal and lesional tissue at a fixed ALA dose, were carried out. For the dose range examined (10–30%), there was no ALA dose dependency of the PpIX fluorescence for any of the lesions tested. Although all three lesions tested did show enhanced PpIX fluorescence as compared with normal skin, there was considerable lesion-to-lesion variability. Thick psoriatic plaques seem to give longer PpIX retention times than those of thin lesions. Limitations of the surface-detected fluorescence methodology are discussed.
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  • 30
    ISSN: 1435-604X
    Keywords: Keywords: Interstitial laser therapy; Liver; Perfusion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Physics , Technology
    Notes: Abstract. Interstitial laser Doppler flowmetry was used to measure the effect of interstitial laser-induced thermotherapy on local blood perfusion in normal rat liver in the peripheral treatment region elevated to hyperthermic temperatures. The Nd:YAG laser emitting at 1064 nm was utilised as heat generation source. The plane-cut tip of an optical fibre was placed in the middle of the exteriorised left liver lobe. Blood perfusion and temperature were measured in the liver parenchyma 4 mm from the laser fibre. The temperature at the location of the liver temperature sensor was maintained at 41 or 44°C during 30 min by regulating the power of the heating laser. The laser Doppler signal was recorded during and after heat treatment, for a total time of 60 min. At 41°C, a significant increase in perfusion up to 1.3 times the initial value was observed 2–16 min after start of treatment. At 44°C, perfusion decreased continuously during and after treatment, and was significantly different from control 40 min after start of treatment. The results may be valuable in assessing the thermal response of tissues surrounding the target in interstitial laser-induced thermotherapy of liver tumours during conditions of normal blood flow.
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  • 31
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    Lasers in medical science 14 (1999), S. 168-174 
    ISSN: 1435-604X
    Keywords: Keywords: Endodontics; Laser ablation; Nd:YAG laser; Picosecond laser; Root canal therapy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Physics , Technology
    Notes: Abstract . The interaction of picosecond laser radiation with human dental tissue was investigated in this study, in order to determine the ablation rates and the surface characteristics of the dentine by using scanning electron microscopy (SEM). Dentine ablation was performed by using tooth sections of different thicknesses (0.5–2.0 mm). Dental tissue samples were irradiated in air with the fundamental wavelength and first harmonic of a regenerative amplifier Nd:YAG laser system, at 1064 nm and 532 nm, respectively, with a pulse duration of 100 ps and a pulse repetition rate of 10 Hz. The results showed very clean craters surrounded by minimum melting of the surface of dentine when the 1064 nm pulses were used. In contrast, when the first harmonic 532 nm pulses were used, the SEM examinations revealed cracks and melting of dentine with irregular surface modification. Consequently, it seems that cleaning and shaping of the root canal walls during endodontic therapy with the picosecond Nd:YAG laser application may be possible in the future. The, as yet unexplored, field of the picosecond laser interaction with hard dental tissue is expected to be a potential alternative for powerful laser processing of biomedical structures.
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  • 32
    ISSN: 1435-604X
    Keywords: Keywords: Chlorins; Firing; Janus green B; Methylene blue; Neuron; Photodynamic effect; Photoheme; Photoporphyrin IX; Photosens; Photosensitisers
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Physics , Technology
    Notes: Abstract . The study of single neuron response to photodynamic effect provides a means for the study of the dynamics of cytotoxic events leading to cell death and allows comparison of the phototoxicity of different photosensitisers. Isolated crayfish stretch receptor neurons were photosensitised for 30 min, then irradiated with a He-Ne laser (632.8 nm; 0.3 W/cm2) until irreversible firing cessation. The dynamics of neuron firing frequency were continuously recorded throughout. The following photosensitisers were studied: methylene blue, janus green B, protoporphyrin IX, chlorins e 6 and p 6, haematoporphyrin derivative (Photoheme) and sulphonated aluminium phthalocyanine (Photosens). Nerve cells were found to be insensitive to either He-Ne laser irradiation or photosensitisation alone, but very vulnerable to the photodynamic effect: neurons changed firing rate and died at nanomolar concentrations of photosensitisers. The dynamics of neuron responses was found to depend on photosensitiser type and concentration. The current approach provides a means of evaluation of initial threshold cell membrane alteration and cytotoxic events leading to cell death. The dependence of firing acceleration rate and neuron lifetime on photosensitiser concentration additionally allowed comparison of efficiencies of different photosensitisers. Photosens, Photoheme and chlorin p 6 were found to be the most potent photosensitisers: neurons responded to their photodynamic effects at concentrations as low as 1–5 nM.
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  • 33
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    Lasers in medical science 14 (1999), S. 73-80 
    ISSN: 1435-604X
    Keywords: Keywords: Biocides; Disinfection; Laser; Optical fibre
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Physics , Technology
    Notes: Abstract. In order to set up a disinfection protocol, the tip of the optical fibre of an Nd-YAP laser, λ=1.34 μm, was contaminated in vitro with: Mycobacterium smegmatis (CIP 7326), Candida albicans (ATCC 2091), Bacillus stearothermophilus (ATCC 7953) or Streptococcus sanguis (ATCC 10556). It was then treated with various biocidal solutions, Septinol® V, glutaraldehyde 2%, ethanol 95° and NaOCl 6%, using one of two protocols: 10 s of contact, or 5 s of contact followed by 5 s of wiping. The latter technique proved to be the most effective. Whatever the biocidal solution used, all the bacteria adhering to the fibre were eliminated. The protocol was checked in vivo in the canals of 10 teeth presenting root canal infections. No bacteria withstood the treatment with Septinol® V. This treatment does not alter the qualities and performance of the fibre as far as light transmission is concerned.
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  • 34
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    Lasers in medical science 14 (1999), S. 105-108 
    ISSN: 1435-604X
    Keywords: Keywords: Glaucoma, open angle; Intraocular pressure; Laser surgery; Trabecular meshwork; Trabeculoplasty
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Physics , Technology
    Notes: Abstract. A randomised prospective study was carried out to determine whether diode laser trabeculoplasty (DLT) is as effective as argon laser trabeculoplasty (ALT) in the control of primary open angle glaucoma (POAG). Forty eyes of 40 patients with uncontrolled intraocular pressure (IOP) despite maximum topical medication were entered into the study. Twenty eyes received DLT, spot size 100 μm, exposure time 0.2 s, mean power 1034 (±56.4) mW. Twenty eyes received ALT, spot size 50 μm, exposure time 0.2 s, mean power 528 (±44.7) mW. They were reviewed at 2 h, 2 and 8 weeks, and 3, 6, 12, 18 and 24 months after laser treatment. Two eyes were withdrawn from the DLT group and four from the ALT group because of uncontrolled IOP during follow-up. Of the remaining eyes in the trial, mean IOP reductions for DLT and ALT, respectively, were: 6 months 7.42 (±2.36) mmHg and 6.36 (±2.3) mmHg; 12 months 7.02 (±2.6) mmHg and 6.28 (±2.2) mmHg; 18 months 6.86 (±2.8) mmHg, 5.98 (±2.02) mmHg; 24 months 6.50 (±2.72) mmHg, 5.92 (±2.02) mmHg. There was a significant reduction in each laser group when IOP at each stage was compared with baseline (p〈0.001) (paired Student's t-test), but no significant difference between the two groups. In the ALT group peripheral anterior synechiae developed in four eyes and post laser pain in seven eyes. These complications were not noted following DLT. We conclude that DLT and ALT are equally effective in IOP control, but DLT is associated with less anterior segment inflammation.
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  • 35
    ISSN: 1435-604X
    Keywords: Keywords: Colour-coded duplex sonography; Laser induced thermotherapy; Minimal invasive therapy; Recurrent breast cancer
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Physics , Technology
    Notes: Abstract. The effect of laser-induced thermotherapy (LITT) as a palliative method for treatment of patients with local recurrence of breast cancer is investigated. This report describes the use of interstitial laser photocoagulation to manage such lesions. The interstitial laser applications were performed in seven women with locally recurrent breast carcinoma on the chest wall after mastectomy. All patients had been heavily pretreated with conventional modes of therapy (radiotherapy, chemotherapy, hormonal therapy, surgical resection). A Nd:YAG laser with a wavelength of 1064 nm was used to heat the lesions. Heat expansion was controlled digitally and monitored by ultrasonography and colour-coded duplex sonography (CCDS). In five women this minimally invasive method enabled the precise coagulation of the subcutaneous tumour without destruction of the skin or ulceration, although these areas had been pretreated by irradiation up to 60 Gy. In two patients with extensive multiple metastases and with skin infiltration, secondary skin ulceration and delayed healing was observed. For palliative reasons, LITT under CCDS guide can aid in local control of chest wall recurrence following mastectomy in selected patients.
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  • 36
    ISSN: 1435-604X
    Keywords: Keywords:Candida albicansCandidosis; Hyphae; Lethal photosensitisation; Toluidine blue O; Yeast
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Physics , Technology
    Notes: Abstract. Oral infections due to Candida albicans are a common occurrence in patients with acquired immunodeficiency syndrome (AIDS). The purpose of this investigation was to determine whether the yeast and hyphal forms of the organism could be killed using the light-activated antimicrobial agent toluidine blue O (TBO). Three variables were investigated: TBO concentration, laser light dose and pre-irradiation time (PIT). Irradiation with light from a helium neon (HeNe) gas laser used in conjunction with the photosensitiser TBO resulted in substantial kills of both the yeast and hyphal forms. Killing was light dose-dependent with 42 J being the most effective dose. The optimum PIT for the yeast form was 5 min, whereas killing of the hyphal form was not affected by PIT. The results of this study have shown that both forms of C. albicans are susceptible to lethal photosensitisation using TBO in conjunction with HeNe laser light, suggesting the possibility that this approach could be useful for eliminating the organism from diseased lesions in vivo.
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  • 37
    ISSN: 1435-604X
    Keywords: Keywords: Beamsplitter; Liver; Metastases; Nd:YAG; Tumour
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Physics , Technology
    Notes: Abstract . Simultaneous application of multiple fibres could increase the volume of coagulation produced with interstitial laser photocoagulation (ILP) for solid tumours. To take full advantage of the presumed synergistic thermal effect between the fibres, the optimal combination of laser power and distance between the fibres was investigated. Four fibres with a cylindrical diffusing tip of 2 cm length were used, coupled to an optical beamsplitter for Nd:YAG light (four channels, maximal variation 9.5%, transmission 〉85%). The distance between the fibres was 1, 1.5, 2, 2.5 , 3 or 4 cm with a power output of either 4, 5, 6 or 7 W/fibre; energy per fibre was constant at 1800 J by adjusting exposure time. After laser application, dimensions of the coagulated lesions were measured. The optimal mutual fibre distance was 2 cm ( p〈0.01) at all power levels. This resulted in lesions with a mean (SD) volume of 44.5 (2.1) cm3 and a largest diameter of 5.1 (0.4) cm at 7 W/fibre. Smaller distances between the fibres resulted in smaller lesions with central carbonisation, whereas larger distances resulted in four separate zones of coagulation. It was concluded that simultaneous application of four interstitial fibres may result in a considerable increase of volume of coagulation. Fibre position and mutual fibre distance determines whether synergism of the coagulative effect occurs.
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    Lasers in medical science 14 (1999), S. 244-246 
    ISSN: 1435-604X
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    Topics: Medicine , Physics , Technology
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    ISSN: 1435-604X
    Keywords: Keywords: Diffuser; Emission profile; Fluorescence imaging; Interstitial laser photocoagulation; Photodynamic therapy
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    Topics: Medicine , Physics , Technology
    Notes: Abstract . Cylindrical light diffusers are commercially available for clinical applications such as photodynamic therapy (PDT) and interstitial laser photocoagulation (ILP). A fluorescence imaging technique has been used to quantify the light distribution produced by each of six different diffuser fibres. The light distribution produced by each device was found to depend on the distance the light propagated in the fluorescent dye solution. At a distance of 1 mm from the diffuser midline, the measured profiles were found to be consistent with published results obtained in air at a similar distance. The three devices intended for PDT utilised scattering particles and reflectors in their construction. The profiles produced by these applicators revealed peaks that were attributed to the reflectors located at their distal tips. By comparison, the two etched ILP fibres displayed either a strong modulation in the profile due to non-uniform etching or a predominant forward peak associated with the conical shape of the tip. We conclude that it is important to take into account the forward-directed light emitted by the diffusers when considering clinical applications using these devices.
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  • 40
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    Lasers in medical science 14 (1999), S. 297-306 
    ISSN: 1435-604X
    Keywords: Keywords: Bioheat transfer equation; ILP; In vivo; LITT; Photocoagulation; Rate kinetics; Temperature distribution; Thermal lesion size; Thermotherapy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Physics , Technology
    Notes: Abstract . An investigation of the temperature response and growth of thermal lesions resulting from in vivo, interstitial laser photocoagulation at long exposures was conducted to assess extended lesion growth characteristics and test the applicability of first order unimolecular rate kinetics (Arrhenius theory) to thermal lesion growth. Irradiations were performed in vivo in rabbit muscle using a continuous 805 nm diode laser source operating at 1.0 W coupled to an optical fibre with a precharred tip (i.e. point heat source). Temperature responses were measured using a linear array of five microthermocouples. Each temperature–time profile was fitted to a solution of the Weinbaum–Jiji bioheat transfer equation (W–J BHTE). Lesions were resected 48 h post-irradiation and the necrosis boundaries were determined histologically. Numerical integration of the Arrhenius damage integral using temperature–time data at the lesion boundary produced corresponding pairs of activation energy and pre-exponential factor (E a, α) consistent with reported values for various other end-points and tissue types. Lesion radii were 6.0±0.6, 8.7±0.4 and 9.7±0.5 mm for 10, 20 and 30 min irradiations respectively. Thermal lesion growth predicted from Arrhenius theory was consistent with experimental results and is non-asymptotic by 30 min. Thermal parameters generally assumed to be constant when solving the W–J BHTE were found to vary with radial distance from the source, presumably due to a temperature dependence.
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    Stochastic environmental research and risk assessment 13 (1999), S. 1-26 
    ISSN: 1436-3259
    Keywords: Key words: Spatiotemporal ; stochastic ; mapping ; Bayes ; entropy ; computational approach ; physical knowledge bases ; epistemology.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Architecture, Civil Engineering, Surveying , Energy, Environment Protection, Nuclear Power Engineering , Geography , Geosciences
    Notes: Abstract This paper is concerned with a computational formulation of the Bayesian maximum entropy (BME) mapping method, which can handle rigorously and efficiently spatiotemporal applications of considerable practical importance. BME is a method of modern geostatistics that can integrate and process physical knowledge that belongs to two major bases: general knowledge (i.e., obtained from general principles and laws, summary statistics and background information), and specificatory knowledge (i.e., obtained through experience with the specific situation). BME allows considerable flexibility regarding the choice of an appropriate spatiotemporal map, offers a complete assessment of the mapping uncertainty and contributes to the scientific understanding of the underlying natural phenomenon. Valuable insight is gained by studying a spatiotemporal data set representing water-level elevations at the Equus Beds aquifer (Kansas). Numerical results show that, as was expected in theory, classical geostatistics analysis is obtained as a special case of the considerably more general BME approach. Moreover, modern geostatistical analysis in terms of BME offers more accurate and informative results in practice, by incorporating various sources of physical knowledge that cannot be processed by the classical methods.
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    Stochastic environmental research and risk assessment 13 (1999), S. 27-47 
    ISSN: 1436-3259
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    Topics: Architecture, Civil Engineering, Surveying , Energy, Environment Protection, Nuclear Power Engineering , Geography , Geosciences
    Notes: Abstract Stochastic environmental risk assessment considers the effects of numerous biological, chemical, physical, behavioral and physiological processes that involve elements of uncertainty and variability. A methodology for predicting health risks to individuals from contaminated groundwater is presented that incorporates the elements of uncertainty and variability in geological heterogeneity, physiological exposure parameters, and in cancer potency. An idealized groundwater basin is used to perform a parametric sensitivity study to assess the relative impact of (a) geologic uncertainty, (b) behavioral and physiological variability in human exposure and (c) uncertainty in cancer potency on the prediction of increased cancer risk to individuals in a population exposed to contaminants in household water supplied from groundwater. A two-dimensional distribution (or surface) of human health risk was generated as a result of the simulations. Cuts in this surface (fractiles of variability and percentiles of uncertainty) are then used as a measure of relative importance of various model components on total uncertainty and variability. A case study for perchloroethylene or PCE, shows that uncertainty and variability in hydraulic conductivity play an important role in predicting human health risk that is on the same order of influence as uncertainty of cancer potency.
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    Stochastic environmental research and risk assessment 13 (1999), S. 66-84 
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    Topics: Architecture, Civil Engineering, Surveying , Energy, Environment Protection, Nuclear Power Engineering , Geography , Geosciences
    Notes: Abstract Velocity variability at scales smaller than the size of a solute plume enhances the rate of spreading of the plume around its center of mass. Macroscopically, the rate of spreading can be quantified through macrodispersion coefficients, the determination of which has been the subject of stochastic theories. This work compares the results of a volume-averaging approach with those of the advection dominated large-time small-perturbation theory of Dagan [1982] and Gelhar and Axness [1983]. Consider transport of an ideal tracer in a porous medium with deterministic periodic velocity. Using the Taylor-Aris-Brenner method of moments, it has been previously demonstrated [Kitanidis, 1992] that when the plume spreads over an area much larger than the period, the volume-averaged concentration satisfies the advection-dispersion equation with constant coefficients that can be computed. Here, the volume-averaging analysis is extended to the case of stationary random velocities. Additionally, a perturbation method is applied to obtain explicit solutions for small-fluctuation cases, and the results are compared with those of the stochastic macrodispersion theory. It is shown that the method of moments, which uses spatial averaging, for sufficiently large volumes of averaging yields the same result as the stochastic theory, which is based on ensemble averaging. The result is of theoretical but also practical significance because the volume-averaging approach provides a potentially efficient way to compute macrodispersion coefficients. The method is applied to a simplified representation of the Borden aquifer.
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    Stochastic environmental research and risk assessment 13 (1999), S. 85-99 
    ISSN: 1436-3259
    Keywords: Key words: Hydraulic diffusivity ; groundwater ; spectral analysis ; stochastic boundaries.
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    Topics: Architecture, Civil Engineering, Surveying , Energy, Environment Protection, Nuclear Power Engineering , Geography , Geosciences
    Notes: Abstract: This study uses the cyclical frequency to develop the mathematical relationship between hydraulic diffusivity and spectral density functions calculated from groundwater level variation. Such relationship can be applied to (1) unsteady state, one-dimensional confined aquifer with time-dependent water level on both end boundaries, and (2) linearized unconfined aquifer with or without vertical recharge. The spectral density functions of groundwater fluctuations are largely affected by the spectral density functions obtained from time-dependent end boundaries and their cross-spectral density functions. Hydraulic diffusivity of an aquifer can be solved by type-curve matching technique at a specified frequency band under the conditions of (1) confined aquifer having equal time-dependent boundaries on both ends, (2) unconfined aquifer having equal time-dependent boundaries on both ends with surface recharge, and (3) unconfined aquifer subjected to surface recharge but neglecting the water table fluctuations on both end boundaries.
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    Stochastic environmental research and risk assessment 13 (1999), S. 113-130 
    ISSN: 1436-3259
    Keywords: Key words: Climatology ; meso-scale convective systems ; classification ; anisotropy ; intermittency ; ergodicity ; upscaling ; level sets.
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    Topics: Architecture, Civil Engineering, Surveying , Energy, Environment Protection, Nuclear Power Engineering , Geography , Geosciences
    Notes: Abstract: The meta-Gaussian model is fitted to a set of 258 sahelian rainfields. The hypotheses underlying this model are discussed with a special emphasis on its ergodic properties, the scale of the phenomenon and the scale of observation. Then the ability of this model to reproduce some observed features, in particular upscaling properties, is checked from a distributional point of view. Finally, some simple properties of the thresholds which are linked to the area threshold method are described.
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    Stochastic environmental research and risk assessment 13 (1999), S. 100-112 
    ISSN: 1436-3259
    Keywords: Key words: Geostatistic ; Gaussian random functions ; anamorphosis ; intermittency ; discontinuous c.d.f ; valid covariance ; internal consistency.
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    Notes: Abstract: For the purpose of numerically studying sahelian storm rainfields, a family of random functions is described with a characterization of its finite dimensional law. Some problems appearing when fitting its functional parameters are put forward and two solutions to bypass those problems are provided, according to the regularity properties of the marginal cumulative distribution function. An illustration of this method is implemented on a set of sahelian rainfields of event accumulation displaying a strong spatial intermittency.
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    Stochastic environmental research and risk assessment 13 (1999), S. 365-379 
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    Notes: Abstract. Conceptual model selection is a key issue in risk assessment studies. We analyze the effect of a number of conceptual aspects related to solute transport in two-dimensional heterogeneous media. The main issues addressed are non-ergodicity, anisotropy in the correlation structure of the transmissivity field, and dispersion at the local scale. In particular, we study the development of a solute plume when mean flow is oriented at an angle with respect to the principal directions of anisotropy. The study is carried out in a Lagrangian framework using Monte Carlo analysis. Of special interest is the evolution of individual plumes. A number of aspects are analyzed, namely the location of the center of mass for each plume and the different ways to compute the angles that the main axes of the plume develop with respect to the direction of the mean flow. Stochastic theories based upon ergodicity conclude that the plume gets oriented in the mean flow direction. In our non-ergodic simulations, the mean of the offset angles, for each individual plume in each particular realization, is offset from the mean flow direction towards the direction of maximum anisotropy. If, instead, the analysis is performed on the ensemble plume (superposition of all different simulations), it is then found oriented closer to the direction of the mean flow than the average offset angle for the different plumes considered separately. This last result adds an extra word of caution to the use of ensemble averaged values in solute transport studies. Serious implications for risk assessment follow from the conceptual model adopted. First, in any single realization there will a large uncertainty in locating the plume at any given time; second, real dilution would be less than what would be expected if the macrodispersion values obtained for ergodic conditions were applied; third, the volume that is affected by a non-zero concentration is smaller than that predicted from macrodispersion concepts; fourth, the orientation of the plume does not correspond to that of the mean flow; and fifth, accounting for local dispersion helps reducing uncertainty.
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    Stochastic environmental research and risk assessment 13 (1999), S. 396-415 
    ISSN: 1436-3259
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    Topics: Architecture, Civil Engineering, Surveying , Energy, Environment Protection, Nuclear Power Engineering , Geography , Geosciences
    Notes: Abstract. We analyze the movement of a solute cloud in a saturated aquifer, resulting from a point-like instantaneous solute injection. Physical heterogeneities of the medium due to spatial variations of the hydraulic conductivity, as well as the chemical heterogeneities due to variations in the linear adsorption coefficient and the degradation rate, are modeled as spatial stochastic processes with exponential autocorrelation functions. Furthermore, cross-correlations between the chemical properties and the conductivity are taken into account. For large transport times, the movement of the solute cloud is characterized by its center-of-mass velocity, by the macroscopic dispersion constant, and the macroscopic degradation rate. These quantities are evaluated using perturbation theory and two different averaging procedures. The first procedure derives the large-scale properties from the central moments of the concentration distribution in a given aquifer realization, and averages over the ensemble afterwards. The second method which is mathematically less demanding obtains large scale transport coefficients from the central moments of the ensemble-averaged concentration distribution. Under the assumption that both prescriptions lead to the same macro-scale quantities, the second approach is usually preferred in literature. The present paper is an extension of the work of Metzger et al. (1996). We show that the two averaging procedures lead to different results in one-dimensional systems, whereas the difference vanishes for higher dimensions. Taking into account the influence of small scale dispersion, we give explicit results for the macroscopic parameters characterizing the solute plume. We analyze the various contributions to these parameters and show how the physical origin of these contributions can be traced back uniquely to fluctuations in the retardation factor, in the flow field, and in the degradation rate, and to the cross-correlations between these inhomogeneities, respectively.
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    Stochastic environmental research and risk assessment 13 (1999), S. 416-435 
    ISSN: 1436-3259
    Keywords: Key words: Sequential linear estimator, successive linear estimator, conditional covariance, interpolation with large data sets.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Architecture, Civil Engineering, Surveying , Energy, Environment Protection, Nuclear Power Engineering , Geography , Geosciences
    Notes: Abstract. A sequential linear estimator is developed in this study to progressively incorporate new or different spatial data sets into the estimation. It begins with a classical linear estimator (i.e., kriging or cokriging) to estimate means conditioned to a given observed data set. When an additional data set becomes available, the sequential estimator improves the previous estimate by using linearly weighted sums of differences between the new data set and previous estimates at sample locations. Like the classical linear estimator, the weights used in the sequential linear estimator are derived from a system of equations that contains covariances and cross-covariances between sample locations and the location where the estimate is to be made. However, the covariances and cross-covariances are conditioned upon the previous data sets. The sequential estimator is shown to produce the best, unbiased linear estimate, and to provide the same estimates and variances as classic simple kriging or cokriging with the simultaneous use of the entire data set. However, by using data sets sequentially, this new algorithm alleviates numerical difficulties associated with the classical kriging or cokriging techniques when a large amount of data are used. It also provides a new way to incorporate additional information into a previous estimation.
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    Meteorology and atmospheric physics 1 (1949), S. 347-357 
    ISSN: 1436-5065
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    Topics: Geography , Physics
    Description / Table of Contents: Zusammenfassung Ein Luftpartikelchen, das ausschließlich adiabatischen Zustandsänderungen unterliegt, nimmt seine Gleichgewichtslage im Innern einer im hydrostatischen Gleichgewicht befindlichen Luftmasse ein, wenn die SummeS seiner spezifischen Enthalpie und seiner potentiellen Energie in der Masseneinheit ein Extrem wird; je nachdem dieses Extrem ein Minimum oder ein Maximum ist, ist das hydrostatische Gleichgewicht stabil oder instabil. Diese Forderung läßt sich für den Fall der geostrophischen Bewegung verallgemeinern, indem man zur SummeS die kinetische Longitudinalenergie der Partikel (je Maßeinheit) addiert. Dieses letztere Resultat läßt sich unmittelbar auf den Fall des permanenten Kreiswirbels ausdehnen.
    Abstract: Summary A particle of air which undergoes but adiabatic changes of state occupies its position of equilibrium inside a mass of air in hydrostatic equilibrium if the sumS of its specific enthalpy and of its potential energy per unit of mass assumes an extreme value. According as this extreme is a minimum or a maximum the state of hydrostatic equilibrium is stable or unstable. This postulate can be generalized in case of geostrophic state of motion on condition that the kinetic longitudinal energy of the particle per unit of mass is added to the sumS. This last result can be immediately extended on the case of a permanent circular vortex.
    Notes: Résumé Une particule qui ne subit que des transformations adiabatiques, occupe sa position d'équilibre au sein d'une masse d'air en équilibre hydrostatique, lorsque la sommeS de son enthalpie spécifique et de son énergie potentielle par unité de masse est extremum. Suivant que cet extremum est un minimum ou un maximum, l'état d'équilibre hydrostatique est stable ou instable. On peut généraliser cette proposition au cas de l'état de mouvement géostrophique, à condition d'ajouter, à la sommeS, l'énergie cinétique longitudinale, par unité de masse, de la particule. On peut étendre immédiatement ce dernier résultat au cas du tourbillon circulaire permanent.
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    Stochastic environmental research and risk assessment 13 (1999), S. 48-65 
    ISSN: 1436-3259
    Keywords: Key words: Excess relative risk model ; two-stage model ; Poisson random variable
    Source: Springer Online Journal Archives 1860-2000
    Topics: Architecture, Civil Engineering, Surveying , Energy, Environment Protection, Nuclear Power Engineering , Geography , Geosciences
    Notes: Abstract Several manuscripts have been published which implement regression methods or stochastic processes methodology to model the incidence of a variety of cancers obtained via epidemiologic studies of radiation exposure. Cancer incidence data obtained from the Life Span Study (LSS) cohort have been of particular interest due to the extensive amount of information available from numerous years of follow-up of the Japanese A-bomb survivors. Conventional methods of exploring goodness-of-fit within each model class have indicated that both types adequately fit the data, but the model predicted curves for excess relative risk (ERR) and excess absolute risk (EAR) can vary dramatically. A simulation study is carried out to compare and contrast an excess relative risk regression model to a stochastic two-stage cancer model to determine the extent of the model predicted risk differences and why they occur.
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    Meteorology and atmospheric physics 1 (1949), S. 384-407 
    ISSN: 1436-5065
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    Topics: Geography , Physics
    Description / Table of Contents: Summary Many cold fronts which cross the principal crest of the Alps in a Southerly direction and penetrate into the region are very avtive in that region. Other cold fronts, however, die out almost completely when crossing the Southern Alps, due to Föhn phenomena. In the present paper, the behaviour of cold air currents crossing the Alps and penetrating the Southern Alps region is thoroughly discussed by analysing individual examples. Thereby it is shown that a clear distinction between the two kinds of cold air invading the Southern Alps region can be drawn from considering the characteristic features of the airstream. Both cases can be easily recognised by examination of the relevant upper air charts. Cold fronts are active in the Southern Alps when this region lies ahead of a high-level trough in the upper air: whilst cold fronts die out through the effect of the Föhn when the Southern Alps lie to the rear of a high-level trough.
    Abstract: Résumé De nombreux fronts froids dirigés vers le Sud et qui traversent les Alpes se révèlent extrêmement actifs sur le versant sud, de cette chaîne. D'autres fronts au contraire disparaissent presque complètement en franchissant le faîte montagneux à cause du phénomène de foehn. On examine ici des cas déterminés de passages de masses d'air froides par-dessus les Alpes et leur écoulement sur le versant opposé. En repérant certaines caractéristiques de ces courants, il est possible de distinguer nettement les deux types d'invasion froide au Sud des Alpes. Cette distinction est particulièrement facile par l'emploid des cartes de pression en altitude. On constate alors qu'un front froid reste actif en traversant les Alpes lorsque la partie antérieure d'un couloir de basse pression en altitude se trouve sur le versant sud, mais qu'il y a frontolyse par foehn lorsque la partie postérieure de ce couloir se trouve sur cette région.
    Notes: Zusammenfassung Viele Kaltfronten, die mit Südkurs den Alpenhauptkamm überschreiten und in das Südalpengebiet eindringen, sind dort außerordentlich wetterwirksam. Andere Kaltfronten, die über die Alpen hinwegziehen, werden dagegen in den Südalpen durch Föhnerscheinungen fast völlig aufgelöst. In vorliegender Arbeit wird an Einzelbeispielen das Verhalten von Kaltluftströmungen beim Überqueren der Alpen und beim Eindringen in das Südalpengebiet eingehend untersucht. Dabei zeigt sich, daß durch charakteristische Merkmale der Strömmungen eine einwandfreie Unterscheidung der beiden Arten von Kaltlufteinbrüchen in das Südalpengebiet möglich ist. Vor allem lassen sich beide Fälle durch Anwendung der Höhenwetterkarte gut auseinanderhalten. Es ergibt sich, daß eine Kaltfront dann die Südalpen wetterwirksam überquert, wenn dieses Gebiet auf der Vorderseite eines in der Höhenkarte in Erscheinung tretenden Höhentroges liegt, daß dagegen die Front durch Föhnwirkung aufgelöst wird, wenn die Südalpen auf der Rückseite des Höhentroges liegen.
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    Meteorology and atmospheric physics 1 (1949), S. 273-294 
    ISSN: 1436-5065
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    Topics: Geography , Physics
    Description / Table of Contents: Summary The paper gives a general view of the investigations dealing with the general circulation of the atmosphere in middle latitudes, carried out by the Department of Meteorology of the University of Chicago under the direction of Prof.C. G. Rossby during the academic year 1946–47. The zonal circulation in the middle latitudes of the northern hemisphere shows, particularly during the winter halfyear and normally between heights of 5 and 15 km, a fairly narrow band of very strong western winds with the character of a free jet. The maximum of intensity of this wind-band appears at the level of the tropopause (300–200 mb.). The wind velocity is rapidly decreasing to the south of the wind maximum. The zonal windband is located within or immediately to the south of an equally narrow zone in which the contrast of temperature between the polar and equatorial regions reaches its highest degree. There is a strong inclination of the tropopause within the zonal wind-band. Frequently, even a discontinuity can be found, the height of the tropopause corresponding to that of the polar atmosphere in the north and to that of the equatorial atmosphere in the south of the wind-band. Below the wind-band, often a well defined frontal zone is situated, ending in the upper layers of the troposphere a little to the north of the wind-band. The upper west-wind belt embracing the whole earth has a wavelike form with wave-lengths from about 50 to 120 degrees of longitude. These waves show but a small velocity of propagation and do not appear to be connected with the ordinary short and unstable frontal waves of the troposphere. On the other hand, they seem to influence considerably the behaviour as well as the movement of these short waves of the lower atmosphere. However, the upper waves are unstable insofar as the amplitude of the oscillation (formation of meander) is little by little increasing so much as to form whirls at the margins of the wind-band: of cyclonic nature and cold thermic structure at the southern side, of anticyclonic nature and warm thermic structure at the northern side. This cutting off of air-masses from within the wind-band causes an exchange of air-masses between high and low latitudes which appears to be of greatest importance in the tropospheric air-layers above 700 mb. Theoretical investigations show that the observed meridional windprofiles at the level of the tropopause agree rather well with those theoretically computed on the assumption of a constant vertical component of absolute vorticity within the lateral large-scale exchange of air-masses in the middle latitudes. Theoretical wind-profiles of this kind show, in low latitudes, such a strong meridional wind-gradient as to cause inertia instability at the southern edge of the wind-band. Due to them, the meridional wind-increase ceases more or less abruptly in latitudes of 40 to 30 degrees. The conclusion can be drawn from the simultaneous appearance of the wind-band and the frontal zone below it that the formation of the west-wind-band must be accompanied by a dynamic concentration of solenoids within the band, a phenomenon in a similar form to be found with oceanic currents. In addition to these important results of recent American researches, shortly indicated above, several Austrian investigations referring to some questions of the discussed problems are mentioned.
    Abstract: Résumé L'auteur donne un aperçu des recherches sur la circulation générale de l'atmosphère sous les latitudes moyennes entreprises par le Department of Meteorology of the University of Chicago, sous la direction du professeurC. G. Rossby en 1946/47. Dans la circulation zonale des latitudes moyennes de l'hémisphère nord apparaît, en hiver surtout, une région assez étroite de très forts vents d'Ouest entre 5 et 15 km. d'altitude qui a l'allure d'un jet aérien délimité. Le maximum de vitesse de ce courant se produit au niveau de la tropopause (300 à 200 mb.); au Sud de ce maximum, la vitesse décroît rapidement. Le courant zonal se trouve dans une bande étroite, ou immédiatement au Sud de celle-ci, dans laquelle les différences de température entre les régions polaires et équitoriales ont leurs plus grandes valeurs. A l'intérieur du courant la tropopause monte rapidement et présente même souvent une discontinuité: au Nord, son altitude correspond à celle de l'atmosphère polaire, au Sud à celle de l'atmosphère équatoriale. Au-dessous du même courant, on rencontre souvent une zone frontale bien développée qui dans les couches supérieures de la troposphère se termine un peu au Nord du courant zonal. La partie supérieure du courant d'Ouest présente une structure ondulée, avec des longueurs d'onde comprises entre 50° et 120° de longitude. Ces ondes ne se déplacent que lentement et ne paraissent pas être de même nature que les ondes frontales instables de la troposphère. D'autre part elles semblent exercer une action décisive sur l'allure et sur le mouvement des courtes ondes frontales inférieures. Les ondes supérieures sont instables en tant que sur leurs bords il peut se produire des ondulations donnant naissance à des tourbillons: sur le bord sud du courant apparaissent des tourbillons cycloniques et froids; sur le bord nord des tourbillons anticycloniques et chauds. Le courant d'Ouest expulse de la sorte des masses d'air et entretient un échange de masses entre les hautes et les basses latitudes qui semble avoir une grande importance dans les couches troposphériques au-dessus de 700 mb. Des recherches théoriques montrent que les profils méridiens du vent observés au niveau de la tropopause concordent fort bien avec ceux qu'exige la constance d'une composante verticale tourbillonnaire dans le cadre de l'échange turbulent à grande échelle des latitudes moyennes. De tels profils théoriques du vent montrent, aux basses latitudes, un gradient méridien du vent tellement grand que des états instables d'inertie doivent se produire au Sud du courant d'Ouest. Ces zones d'instabilité introduisent une cessation plus ou moins brusque de l'accroissement méridional de vitesse du vent vers 40° à 30° de latitude. Le fait que le courant d'Ouest étroit se superpose à une zone frontale inférieure amène à conclure que la formation de ce courant est accompagnée d'une concentration dynamique de solénoïdes à son niveau, phénomène analogue à celui que l'on observe dans les courants marins. Faisant suite à cet examen des travaux américains, l'article énumère encore quelques résultats de recherches autrichiennes relatives à certaines questions du même problème.
    Notes: Zusammenfassung Im folgenden wird ein Überblick über die Untersuchungen über die allgemeine Zirkulation der Atmosphäre in den mittleren Breiten der Erde gegeben, die vom Department of Meteorology of the University of Chicago unter der Leitung von Prof.C. G. Rossby im Akademiejahr 1946 bis 1947 ausgeführt worden sind. In der zonalen Zirkulation der mittleren Breiten der Nordhemisphäre zeigt sich vor allem im Winterhalbjahr normalerweise zwischen 5 und 15 km Höhe ein verhältnismäßig schmales Band von sehr starken Westwinden, das den Charakter eines Freistrahls besitzt. Das Intensitätsmaximum dieses Windbandes tritt im Tropopausenniveau (300 bis 200 mb) auf. Südwärts des Windmaximums nimmt die Windgeschwindigkeit sehr rasch auf niedrige Werte ab. Das zonale Windband liegt innerhalb oder knapp südlich einer ebenfalls relativ schmalen Zone, in der der Temperaturgegensatz zwischen den polaren und äquatorialen Gebieten konzentriert ist. Im Bereich des zonalen Windbandes steigt die Tropopause steil an, wobei häufig sogar eine Unstetigkeit auftritt, indem nördlich desselben ihre Höhe jener der polaren Atmosphäre entspricht, südlich davon jener der äquatorialen. Unter dem Windband liegt häufig eine gut ausgebildete Frontalzone, die in den oberen Troposphärenschichten etwas nördlich des Windbandes endigt. Das obere Westwindband zeigt rund um die ganze Erde einen wellenförmigen Verlauf mit Wellenlängen von etwa 50° bis 120° Längengraden. Diese Wellen weisen nur eine geringe Fortpflanzungsgeschwindigkeit auf und scheinen mit den gewöhnlichen kurzen, instabilen troposphärischen Frontalwellen keine nähere Beziehung zu haben. Anderseits scheinen sie sowohl das Verhalten, wie auch die Bewegung dieser kurzen unteren Wellen in maßgebender Weise zu beeinflussen. Die oberen Wellen sind insofern instabil, als die meridionale Schwingungsweite (Mäanderung) allmählich sich so verstärkt, daß es an den Rändern des Windbandes zur Bildung von Wirbeln kommt: auf der Südseite zyklonischer Natur und kalten thermischen Aufbaues, auf der Nordseite antizyklonischer Natur und warmen thermischen Aufbaues. Diese Ausstoßung von Luftmassen aus dem Windbandbereich führt zu einem Luftmassenaustausch zwischen hohen und niedrigen Breiten, der wie es scheint in den troposphärischen Schichten oberhalb 700 mb von ausschlaggebender Bedeutung ist. Theoretische Untersuchungen zeigen, daß die beobachteten meridionalen Windprofile im Tropopausenniveau recht gut mit solchen übereinstimmen, die der Forderung einer konstanten vertikalen Wirbelkomponente im Bereich des großräumigen seitlichen Austausches der mittleren Breiten genügen. Solche theoretische Windprofile zeigen in niedrigen Breiten ein so großes meridionales Windgefälle, daß es am Südrand des Windbandes zu Trägheitsinstabilitäten kommt. Sie führen zu einem mehr oder minder unvermittelten Abbruch der meridionalen Windzunahme in 40° bis 30° Br. Das gleichzeitige Auftreten von Windband und Frontalzone darunter führt zur Schlußfolgerung, daß es bei der Ausbildung des Westwindbandes zu einer dynamischen Konzentration von Solenoiden in seinem Bereich kommen muß, zu ähnlichen Vorgängen, wie sie bei ozeanischen Strömen auftreten. Im Anschluß an diese hier kurz angedeuteten wichtigen Ergebnisse dieser neueren amerikanischen Arbeiten wird auf einige Ergebnisse österreichischer Untersuchungen hingewiesen, die einige Fragen der obigen Probleme betreffen.
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    Journal of geographical systems 1 (1999), S. 1-2 
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    Topics: Geography
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    Journal of geographical systems 1 (1999), S. 3-22 
    ISSN: 1435-5949
    Keywords: Key words: Classification ; neural networks ; G15 ; JEL classification: C88 ; C63 ; C45 ; C44
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geography
    Notes: Abstract. Neural Networks are now established computational tools used for search minimisation and data classification. They offer some highly desirable features for landuse classification problems since they are able to take in a variety of data types, recorded on different statistical scales, and combine them. As such, neural networks should offer advantages of increased accuracy. However, a barrier to their general acceptance and use by all but `experts' is the difficulty of configuring the network initially.  This paper describes the architectural problems of applying neural networks to landcover classification exercises in geography and details some of the latest developments from an ongoing research project aimed at overcoming these problems. A comprehensive strategy for the configuration of neural networks is presented, whereby the network is automatically constructed by a process involving initial analysis of the training data. By careful study of the functioning of each part of the network it is possible to select the architecture and initial weights on the node connections so the constructed network is `right first time'. Further adaptations are described to control network behaviour, to optimise functioning from the perspective of landcover classification. The entire configuration process is encapsulated by a single application which may be treated by the user as a `black box', allowing the network to the applied in much the same way as a maximum likelihood classifier, with no further effort being required of the user.
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    Journal of geographical systems 1 (1999), S. 37-60 
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    Keywords: Key words: Computational intelligence ; glacier hydrology ; genetic programming ; neural networks ; fuzzy logic ; self-organizing map ; JEL classification: C61 ; C63 ; C80
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geography
    Notes: Abstract. Measurements of water pressure beneath Trapridge Glacier, Yukon Territory, Canada show that the basal water system is highly heterogeneous. Three types of behaviour were recorded: pressure records which are strongly correlated, records which are strongly anticorrelated, and records which alternate between strong correlation and strong anticorrelation. We take the pressure in bore-holes that are connected to the evacuation route for basal water as the forcing, and the other pressures as the response to this forcing. Previous work (Murray and Clarke 1995) has shown that these relationships can be modelled using low-order nonlinear differential equations optimized by inversion. However, despite optimizing the model parameters we cannot be sure that the final model forms are themselves optimal. Computational intelligence techniques provide alternative methods for fitting models and are robust to missing or noisy data, applicable to non-smooth models, and attempt to derive optimal model forms as well as optimal model parameters. Four computational intelligence techniques have been used and the results compared with the more conventional mathematical model. These methods were genetic programming, artificial neural networks, fuzzy logic and self-organizing maps. We compare each technique and offer an evaluation of their suitability for modelling the pressure data. The evaluation criteria are threefold: (1) goodness of fit and an ability to predict subsequent data under different surface weather conditions; (2) interpretability, and the extent and significance of any new insights offered into the physics of the glacier; (3) computation time. The results suggest that the suitability of the computational intelligence techniques to model these data increases with the complexity of the system to be modelled.
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    Journal of geographical systems 1 (1999), S. 107-136 
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    Keywords: Key words: Statistical analysis ; spatial objects ; surface ; distribution ; JEL classification: C12 ; C14 ; C69
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    Topics: Geography
    Notes: Abstract. This paper develops statistical methods for analyzing the distribution of spatial objects—points, convex polygons, and line segments—in relation to a surface. We propose statistics for measuring the relationship between the distribution of these objects and a surface and derive their expectations and variances under the null hypothesis that the objects are independently and randomly distributed. The statistics are approximately distributed according to the normal distribution under the null hypothesis, which enables us to test the significance of the spatial relationships statistically. Using the proposed methods, we empirically analyze the distribution of convenience stores in relation to the distribution of population in a suburb of Osaka, Japan. Some empirical findings are shown.
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    Journal of geographical systems 1 (1999), S. 23-35 
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    Keywords: Key words: Remote sensing ; fuzzi classification ; boundaries ; neural network ; JEL classification: C45 ; Q24 ; Q20
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geography
    Notes: Abstract. Remote sensing is the only feasible means of mapping and monitoring land cover at regional to global scales. Unfortunately the maps are generally derived through the use of a conventional 'hard' classification algorithm and depict classes separated by sharp boundaries. Such approaches and representations are often inappropriate particularly when the land cover being represented may be considered to be fuzzy. The definition of boundaries between classes can therefore be difficult from remotely sensed data, particularly for continuous land cover classes which are separated by a fuzzy boundary which may also vary spatially in time. In this paper a neural network was used to derive fuzzy classifications of land cover along a transect crossing the transition from moist semi-deciduous forest to savanna in West Africa in February and December 1990. The fuzzy classifications revealed both sharp and gradual boundaries between classes located along the transect. In particular, the fuzzy classifications enabled the definition of important boundary properties, such as width and temporal displacement.
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    Papers in regional science 78 (1999), S. 243-263 
    ISSN: 1435-5957
    Keywords: JEL classification: R41 ; Key words:Logistics cost, consumer demand, retail establishment density, terminal, demand-supply interaction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geography , Economics
    Notes: Abstract. This article develops models to formulate the optimal density of retail establishments by considering interactions between logistics cost and consumer demand. Commodities are assumed to be distributed from a depot directly or through single intermediate terminal to many retail establishments. Average logistic cost per item, consumer demand, and the interrelationship between them are analyzed. The optimal density of retail establishments and local terminals are determined by minimizing average logistic cost, or maximizing total supply subject to the demand-supply equality. The envelope curves for the optimal configuration strategies corresponding to different values of total market area and terminal cost are derived.
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    Papers in regional science 78 (1999), S. 297-318 
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    Keywords: JEL classification: R11 ; Key words:Information sector, specialization, city types
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    Topics: Geography , Economics
    Notes: Abstract. The national economy is split into four broad sectors. The observed variation in long-term growth among sectors leads to a shift in the composition of aggregate demand. I test Henderson's propositions, namely that secular shifts in the composition of national demand lead to changes in the numbers and sizes of different types of urban places. The numbers and sizes of places specialized in the information sector has increased while the numbers and sizes of places specialized in manufacturing has decreased or has not changed. Metropolitan population growth and per capita earnings growth are enhanced by specialization in the information sector.
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    Journal of geographical systems 1 (1999), S. 137-153 
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    Keywords: Key words: Geographic information systems ; land use allocation ; generalized assignment problem ; multiobjective analysis ; JEL classification: Q15 ; Q24 ; R14 ; R52
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geography
    Notes: Abstract. As geographic information systems (GIS) have moved from information storage and retrieval operations towards more decision support functions, there is a need for more integration of spatial analytical modules that can assist in locational decisions. This paper presents a methodology for coupling land use allocation models with a raster GIS. For raster systems, the integration of any decision module has been limited by the size of raster datasets that may contain hundreds of thousands of pixels. Therefore, decision heuristics have been used rather than exact methods such as mathematical programming models. For the problem of land use allocation, the special structure of the generalized assignment problem is used here to handle large scale datasets. The advantage of the mathematical programming approach is the additional information associated with the dual variables and opportunity costs that can be used in subsequent sensitivity analyses.
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    Lasers in medical science 14 (1999), S. 20-23 
    ISSN: 1435-604X
    Keywords: Keywords: Diode laser; Expandable metal stent; Malignant dysphagia; Nd-YAG; Overgrowth; Tumour in-growth
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Physics , Technology
    Notes: Abstract. Re-establishment of the oesophageal lumen is the main focus of care in patients with dysphagia due to re-blockage of in situ expandable metal stent (EMS). A total of 51 patients aged 44–89 years were intubated with EMS for dysphagia due to inoperable oesophagogastric carcinoma. Of these patients, 25 required follow-up endoscopy at variable intervals after stent insertion; 17 patients were found to have significant tumour in-growth (9), overgrowth (4) or both (4). All these patients were treated with Nd-YAG (70 W/s) or diode laser (30–50 W, continuous) for maintenance of satisfactory swallowing. The intensity of tumour ablation was similar with both types of lasers but four patients being treated with Nd-YAG laser developed deformity of EMS. This complication was not encountered with diode laser. The timing of the stent insertion should be carefully chosen since the longer the stent is in situ, the greater is the likelihood of tumour ingrowth or overgrowth. The combination of endoscopic laser therapy (ELT) and EMS may offer the best palliation, particularly when patient survival of several months is anticipated. ELT can effectively deal with tumour in-growth and overgrowth but care must be exercised in the use of Nd-YAG which can damage the structure of the EMS.
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    Lasers in medical science 14 (1999), S. 86-90 
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    Keywords: Keywords: Benign prostatic hyperplasia; Enucleation; Holmium; Laser; Prostatectomy; Resection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Physics , Technology
    Notes: Abstract. At a wavelength of 2140 nm, the holmium:YAG (Ho:YAG) laser can be used for incision, ablation and resection of prostatic tissue. This laser has revolutionised the surgical management of benign prostatic hyperplasia (BPH) in our institution. Five different techniques have been utilised in evolving the current techniques using the Ho:YAG laser either alone or in combination with neodymium:YAG (Nd:YAG laser for the surgical management of BPH. The clinical outcome of 664 patients undergoing these procedures is presented. Enucleation of the prostatic adenoma using the holmium laser energy is the most common procedure presently performed at our institution for the surgical management of BPH. A mechanical tissue morcellator appears to be the most efficient way to remove prostatic tissue, once enucleated, from within the bladder.
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    Lasers in medical science 14 (1999), S. 129-135 
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    Keywords: Keywords: Microcirculation; Mono-l-aspartyl chlorin-e-6 (Npe-6); Photodynamic therapy (PDT); Vascular shut-down
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Physics , Technology
    Notes: Abstract. An experimental system that allows the white light observation of rapid changes in vessels without disturbance by red laser light was used. Mice were injected with mono-l-aspartyl chlorin-e-6 (Npe-6) i.v. via the tail vein and were immediately exposed to laser light. White emboli were observed forming on the inside of the vessel walls within seconds after commencement of light exposure. Emboli adhered to vessel walls and caused vascular obstruction. Light microscopy of the exposed material using fibrin staining was performed. Electron microscopy on the same material was also carried out. The embolisation time was influenced by both drug dose and laser power. With low laser power, it took a long time to stop the blood flow. Fibrin staining revealed the white emboli to be composed of fibrin. Electron microscopy findings revealed damage to endothelial cells and platelet aggregation. This study suggests that two main mechanisms (direct cellular damage and vascular shut-down ) might actually be complementary and synergistic in the production of vascular lesions using photodynamic therapy.
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    Lasers in medical science 14 (1999), S. 167-167 
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    Lasers in medical science 14 (1999), S. 175-176 
    ISSN: 1435-604X
    Keywords: Keywords: Laser; Spider naevus
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    Topics: Medicine , Physics , Technology
    Notes: Abstract . A total of 112 spider naevi in 86 patients were treated with the tunable dye laser with a wavelength of 585 nm and pulse duration of 450 µs (SPTL 1, Candela Corporation, Wayland, MA). Complete ablation of the spider telangiectasia was achieved in 81 lesions (72.3%) partial ablation was achieved in 27 lesions (24.1%) and four (3.6%) lesions had no response to treatment. Transient hyperpigmentation was noted in one patient and minimal scarring in the form of a slight depression over the treated area was noted in another patient. The pulsed dye laser is shown to be effective in the treatment of spider naevi.
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    Keywords: Keywords: Carbon dioxide laser; Epulis fissuratum; Laser; Vestibuloplasty
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    Topics: Medicine , Physics , Technology
    Notes: Abstract . There have been numerous surgical techniques developed for removing the epulis fissuratum lesions in order to improve alveolar ridge contour and improving adaptation of full prostheses. Most of these techniques can cause postoperative complications, such as oedema, pain, difficulty in swallowing and jaw movements, haemorrhage, infections and slow placement of final prostheses. The use of carbon dioxide (CO2) laser for the surgical removal of epulis lesions has resulted in many significant improvements including convenient mucosa removal, no bleeding or need for sutures, and minimal postoperative pain and oedema. This study is of 15 cases of removal of extensive epulis with vestibuloplasty in the maxilla and mandible that was carried out with CO2 laser, with no postoperative complications, rapid healing, and excellent aesthetic and functional outcome, all of which allowed for more rapid placement of final prosthesis.
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    Keywords: Keywords: Feedback control; Indocyanine green dye; Laser-activated solid protein solder strips; Nerve repair; Temperature monitoring; Tensile strength
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    Topics: Medicine , Physics , Technology
    Notes: Abstract . Laser-activated solid protein solder strips have been developed for peripheral nerve repair. Indocyanine green dye added to the solder strongly absorbs diode wavelengths (∼800 nm) and causes localised heating and coagulation of the albumin protein solder. The protein strengthens the tissue join, particularly during the acute healing phase postoperative, while shielding the underlying axons from excessive thermal damage. In this investigation of the solid protein solder technique for nerve repair, the effect of laser irradiance on weld strength and solder and tissue temperature were studied. The tensile strength of repaired nerves rose steadily with increased irradiance reaching a maximum of 105±10 N/cm2 at 12.7 W/cm2. At higher irradiances, tensile strength fell. The maximum temperature reached at the solder surface and at the solder/nerve interface, measured using a non-contact fibre optic radiometer and thermocouple, respectively, also rose steadily with laser irradiance. At 12.7 W/cm2, the temperatures reached at the surface and at the interface were 88±5°C and 71±4°C, respectively. This in vitro investigation demonstrates the feasibility of the laser-activated solid protein solder strips for peripheral nerve repair. The laser irradiance and the corresponding solder surface temperature for optimal tensile strength have been identified.
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  • 69
    ISSN: 1435-604X
    Keywords: Keywords: Adenocarcinoma; Aminolaevulinic acid; Barrett's oesophagus; Dysplasia; Photodynamic therapy
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    Topics: Medicine , Physics , Technology
    Notes: Abstract . Photodynamic therapy (PDT) may have a role in the prevention of oesophageal cancer. Ten patients with Barrett's oesophagus, three with low-grade dysplasia (LGD), four with high-grade dysplasia (HGD), one with carcinoma in situ and two with invasive carcinoma, were treated with PDT. All received 30 mg/kg aminolaevulinic acid (ALA) followed 4 h later by laser endoscopy. Half were treated with red light (630 nm; 100 mW/cm2 for 1000 s) and half with green light (514 nm; 100 mW/cm2 for 500 s). Columnar epithelial regression was seen in all patients with dysplasia (mean area decrease 44%; range 10–100%), with apparent elimination of dysplasia in all cases. In patients with in situ or invasive carcinoma, no response was seen. ALA-induced PDT, using either red or green light, produces effective ablation of dysplastic Barrett's oesophagus, hence may have a role in the prevention of oesophageal carcinoma, but has little effect on in situ or invasive adenocarcinoma.
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    Biomedical microdevices 2 (1999), S. 91-92 
    ISSN: 1572-8781
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    ISSN: 1572-8781
    Keywords: tumor pathology semiconductor microfabrication microfluidics nanolasers ; nanotechnology cytometry
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    Topics: Medicine , Technology
    Notes: Abstract Through recent interdisciplinary scientific research, modern medicine has significantly advanced the diagnosis and treatment of disease. However, little progress has been made in reducing the death rate due to cancer, which remains the leading cause of death in much of the world. Pathologists routinely rely on microscopic examination of cell morphology using methods that originated over a hundred years ago. These staining methods are labor-intensive, time-consuming, and frequently in error. New micro-analytical methods1 (JBM, 1998; Harrison et al., 1993; Ramsey et al., 1995; Mauro Ferrari, Lynn Jelinski, 1994; Anderson et al., 1996; Carlson et al., 1996) for high speed (real time) automated screening of tissues and cells are critical to advancing pathology and hold the potential for improving diagnosis and treatment of cancer patients. By teaming experts in semiconductor physics, microfabrication, surface chemistry, film synthesis, and fluid mechanics with microbiologists and medical doctors, we are investigating nanostructured biochips to assess the condition of tumor cells by quantifying total protein content. This technique has the potential to quickly identify a cell population that has begun rapid protein synthesis and mitosis, characteristic of tumor cell proliferation. By incorporating microfluidic flow of cells inside the laser microcavity for the first time, we have enabled high throughput screening of cells in their native state, without need of chemical staining, in a sensitive nanodevice.
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    Biomedical microdevices 1 (1999), S. 97-98 
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    Biomedical microdevices 1 (1999), S. 107-112 
    ISSN: 1572-8781
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Technology
    Notes: Abstract The use of microfabricated DNA analysis tools utilizing microfluidics will provide the next generation of inexpensive DNA diagnostics. It will also provide methodologies to measure gene expression in a massively parallel manner, eventually providing the methodologies to measure most or all the human genes of significance on a single chip. These technologies, including PCR analysis, electrophoresis and gene chips are described using examples from the archival literature.
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    Biomedical microdevices 1 (1999), S. 139-153 
    ISSN: 1572-8781
    Keywords: array biosensor ; automation ; CCD imaging ; fluorescence imaging ; antibody patterning ; waveguide
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    Notes: Abstract Optical and fluidics systems have been developed as central components for an automated array biosensor. Disposable planar waveguides are patterned with immobilized capture antibodies using a physically isolated patterning (PIP) method. The PIP method enables simultaneous deposition of several antibodies and completely circumvents cross-immobilization problems encountered with other array deposition processes. A multi-channel fluidics cell allows numerous assays to be performed on the patterned waveguide. The sensing arrays are optically interrogated using a diode laser with a tailored output to optimize coupling to and maximize excitation uniformity within the waveguide. A patterned cladding is employed to optically isolate the waveguide from perturbations induced by the permanently attached flow cells. Compact optics image the evanescently excited fluorescence onto a large area, cooled CCD array. The image data is processed and automated signal analysis corrects for local background and noise variations.
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  • 75
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    Keywords: drug discovery ; CellChip ; high content screening ; fluorescence ; patterning ; sensors ; microarrays ; bioinformatics ; tissue engineering
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    Notes: Abstract A major bottleneck to the early stages of drug discovery is the absence of integration of high throughput screening (HTS) with smarter assays that screen “hits” from HTS to identify leads (High content screening, HCS). We propose a solution using novel fluorescent engineered protein biosensors integrated into a miniaturized live-cell-based screening platform (CellChip™ System) that markedly shortens the early drug discovery process. Microarrays of selectively localized living cells, containing engineered fluorescent biosensors, serve to integrate HTS and HCS onto a single platform. HTS “hits” are identified using one biosensor while reading the whole chip array of cells. The high-biological content information is then obtained from probing target activity at inter-cellular, sub-cellular and molecular levels in the “hit” wells. HCS assays yield temporal-spatial dynamic maps of the drug-target interaction within each living cell. We predict that a new platform incorporating HTS and HCS assays that are automated, miniaturized, and information-rich will dramatically improve the decision making process in the pharmaceutical industry and optimize lead compounds during the early part of the drug discovery process. There is an opportunity to establish a new paradigm for drug discovery based on integration of fluorescence technology, micropatterning of living cells, automated optical detection and data analysis, and a new generation of knowledge building bioinformatics approaches. The technology will have an expansive impact spanning the fields of drug discovery, biomedical research, environmental monitoring, life sciences, and clinical diagnostics. The integrated CellChip™ Platform with miniaturized tissue-specific microarrayed cells capable of providing inter-cellular and sub-cellular spatio-temporal information in response to drug-cell, toxin-cell, or pathogen-cell interactions will serve to enhance the decision making process in drug discovery, toxicology, and clinical diagnostics.
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    Biomedical microdevices 2 (1999), S. 145-150 
    ISSN: 1572-8781
    Keywords: electroporation ; micro-electroporation chip ; cell membrane electrical currents
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    Topics: Medicine , Technology
    Notes: Abstract Electroporation is commonly used in biotechnology to introduce macromolecules into cells. We have developed a micro-electroporation chip that incorporates a live biological cell in the electrical circuit. The chip configuration forces electrical currents to pass through the cell, thereby producing electrically measurable information about the electroporation state of the cell. The cell membrane electrical properties make the cell function as a diode in the electroporation current-voltage range. The chip is transparent in the area of the cell to allow microscope viewing. during electroporation. This chip may be used to study the fundamental biophysics of cell electroporation and in biotechnology for controlled macromolecule introduction in individual cells. We describe the chip principle and show results on the electrical current-voltage pattern during reversible and irreversible electroporation in individual cells.
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  • 77
    ISSN: 1572-8781
    Keywords: MPC ; BMA ; AMPS ; biocompatibility ; cellulose triacetate ; diffusion-limiting effect ; glucose sensor ; needle type ; glucose oxidase ; serum ; whole blood
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    Notes: Abstract A multifunctional membrane with biocompatibility, diffusion-limiting effect, and the ability to curtail the responses of an H2O2 electrode to ascorbate and urate was prepared. It was composed of MB, AB, and CTA, where MB is the copolymer of 2-methacryloyloxyethyl phosphorylcholine (MPC) and n-butylmethacrylate (BMA), AB is the copolymer of acrylamide-2-methylpropane sulfonic acid (AMPS) and BMA, CTA is cellulose triacetate. Investigation of the biocompatibility of this membrane showed that, compared with CTA, relatively few platelets bound to it. The membrane was coated onto the working electrode of a needle-type glucose sensor on which immobilized glucose oxidase membrane has been coated. The sensor did not respond to ascorbate and urate at their concentration normally encountered in blood. Its response was not inhibited by metal ions in blood at usual concentration. The sensor exhibited superior thermostability in addition to a rapid response (〈90 seconds in batch operation), good reproducibility (RE〈5%), good stability (more than 36 hours continuously in heparinized whole blood), and a wide dynamic range (5–650 mg/dl glucose). The sensor was used to determine glucose in serum. The data obtained from the sensor showed good agreement with that from a clinical autoanalyzer (R=0.973).
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    Biomedical microdevices 2 (1999), S. 87-88 
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  • 79
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    Keywords: nondestructive testing ; evanescent microwave imaging ; quantitative testing ; conductivity in biomaterials ; high resolution imaging ; material non-uniformity
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    Notes: Abstract Scanning tunneling and atomic force microscopes (STM and AFM) are used to study biological materials. These methods, often capable of achieving atomic resolutions, reveal fascinating information regarding the inner workings of these materials. However, both STM and AFM require physical contact to the specimen. In the case of STM, the specimen needs to be conducting as well. Here we introduce a new method for imaging biological materials through air or a suitable liquid using decaying or evanescent fields at the tip of a properly designed microwave resonator. This novel method involves the use of an evanescent microwave probe (EMP) and is capable of imaging a variety of non-uniformities in biological materials including conductivity, permittivity, and density variations. EMP is a non-contact and non-destructive sensor and it does not require conducting specimens. Its spatial resolution is currently around 0.4 μm at 1 GHz. We have used this probe to map non-uniformities in a variety of materials including metals, semiconductors, insulators, and biological and botanical samples. Here we discuss applications of EMP imaging in bone, teeth, botanical, and agricultural specimens.
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    Biomedical microdevices 1 (1999), S. 121-130 
    ISSN: 1572-8781
    Keywords: electrochemical actuation ; micropump ; liquid dosing ; gas bubbles
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    Topics: Medicine , Technology
    Notes: Abstract In this paper a micromachined electrochemically driven pump capable of dosing precise nanoliter amounts of liquid is presented. The pump consists of a micromachined channel structure realized in silicon by reactive ion etching. On top of this structure a Pyrex® cover piece with noble metal electrodes was bonded. The fluid to be dispensed is stored in a meander shaped reservoir which is part of the channel structure. This meander starts in an electrolyte solution containing reservoir, on top of which two noble metal electrodes are positioned. By the electrochemical production of gas bubbles by electrolysis of water at these electrodes, liquid can be driven out of the meander. The measured volume displacements were in close agreement with theory. Pump rates as low as a few nl/s could accurately be controlled via the actuation current through the electrodes. By applying current pulses rather than a continuous current, preset amounts of fluid in the nanoliter range could be dosed successfully. Because the resulting device consists of simple channel structures and metal electrodes it can easily be integrated in miniaturized chemical analysis systems to dose reagents or calibration solutions.
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    Biomedical microdevices 2 (1999), S. 9-10 
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    Biomedical microdevices 2 (1999), S. 51-60 
    ISSN: 1572-8781
    Keywords: magnetoelastic ; remote query ; microdevice ; sensor ; glucose
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Technology
    Notes: Abstract Magnetoelastic thin-film sensors can be considered the magnetic analog of an acoustic bell: in response to an externally applied magnetic field impulse the sensors ring like a bell, emitting magnetic flux with a characteristic resonant frequency. The magnetic flux can be detected remotely, external to the test area, using a pick-up coil. By monitoring changes in the characteristic resonant frequency of the sensor multiple environmental parameters can be measured. In this work we report on application of magnetoelastic sensors for remote query measurement of temperature, pressure, viscosity and, in combination with a glucose-responding mass-changing polymer, in situ measurement of biological-level glucose concentrations. The advantage of using magnetoelastic sensors is that they are monitored remotely, without the need for direct physical connections such as wires or cables, nor line-of-sight alignment as needed with optical detection methods. The remote query capability allows the magnetoelastic sensors to be monitored from inside sealed, opaque containers. Depending upon the application magnetoelastic sensors can be sized from micrometer to millimeter dimensional scales, and have a material cost of approximately $0.001 allowing for their use on a disposable basis.
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  • 83
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    Keywords: cell sorting ; travelling wave dielectrophoresis ; filed-flow-fractionation ; computer microvision
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    Topics: Medicine , Technology
    Notes: Abstract We describe a microfluidic device for separating cells according to their dielectric properties by combining 2-dimensional dielectrophoretic forces with field-flow-fractionation. The device comprises a thin chamber in which a travelling-wave electrical field is generated by a planar, multilayer microelectrode array at the bottom. Under the balance of gravitational and dielectrophoretic levitation forces, cells introduced into the device are positioned at different equilibrium heights in a velocity profile established inside the chamber, and thereby transported at different velocities by the fluid. Simultaneously, cells are subjected to a horizontal travelling-wave dielectrophoretic force that deflects them across the flow stream. The 2-dimensional dielectrophoretic forces acting on cells and the associated velocities in the fluid-flow and travelling-field directions depend sensitively on cell dielectric properties. The responses of cultured MDA-435 human breast cancer, HL-60 human leukemia and DS19 murine erythroleukemia cells, and of peripheral blood mononuclear (PBMN) cells were studied as functions of the frequency and voltage of the applied electric signals, and of the fluid flow rate. Significant differences were observed between the responses of different cell types. Cell separation was demonstrated by the differential redistribution of MDA-435 and PBMN cells as they flowed through the device. The device can be readily integrated with other microfluidic components for microscale sample preparation and analysis.
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    Cellular and molecular life sciences 5 (1949), S. 403-404 
    ISSN: 1420-9071
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    Topics: Biology , Medicine
    Notes: Summary The authors have isolated from the intestinal content of the larvae of the waxmothGalleria mellonella a bacterium utilizing beeswax.
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    Cellular and molecular life sciences 5 (1949), S. 407-409 
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    Notes: Zusammenfassung Die Autoren bestimmen den Histamingehalt von Meerschweinchenlungen nach Anlegung eines Pneumothorax, nach lokaler Reizung mit Papain und bei normalen Kontrolltieren. Der Histamingehalt sinkt zunächst sofort nach Herstellung eines Pneumothorax, steight aber nach einigen Stunden wieder an und bleibt während mehrerer Tage über den Normalwert erhöht. Die Reizung der Lunge durch eine lokale Injektion von Papain gibt, ähnlich wie der Pneumothorax, eine nach einiger Zeit auftretende Erhöhung des Histamingehalts.
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    Cellular and molecular life sciences 5 (1949), S. 410-411 
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    Notes: Zusammenfassung Die Wirkung von Ultraschall auf isoliertes Muskelgewebe wird untersucht und dabei folgen des gefunden: Im quergestreiften Muskel, in dem das Actomyosin nicht in der üblichen Weise entfernt worden ist, spalten sich die Muskelfibrillen in der Längsachse. Nach Entfernung des Actomyosins gibt es Aufspaltungen senkrecht zur langen Achse der Fibrillen. Die entstehenden parallelen Spalte liegen in Abständen von 4–8 µ. Im glatten Muskel gibt es, in Abständen von 3–4 µ, senkrecht zu den Fibrillen verlaufende Spalte, auch ohne da\ das Actomyosin entfernt worden ist. Der Herzmuskel wird durch Ultraschall nicht nennenswert beeinflu\t. Die Bedeutung der Befunde wird diskutiert.
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    Cellular and molecular life sciences 5 (1949), S. 417-425 
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    Cellular and molecular life sciences 5 (1949), S. 479-480 
    ISSN: 1420-9071
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    Topics: Biology , Medicine
    Notes: Summary The viscosity of synovial fluids and hyaluronic acid solutions, obtained from vitreous humour, are lowered by ultrasonic vibrations. Electrophoretic analysis reveals that the content of hyaluronic acid in these ultrasonically treated solutions is diminished.
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    Cellular and molecular life sciences 5 (1949), S. 483-484 
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    Notes: Summary In rabbits it has been confirmed, that, if the blood pressure falls rapidly, a lower limit will be attained, below which a normal respiration will no longer exist. This minimal arterial tension seems to be an absolutely fixed one, since it was found to be the same, whether the respiratory center was normal or under the influence of either a depressing or a stimulating drug.
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    Cellular and molecular life sciences 5 (1949), S. 444-444 
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    Notes: Summary A phage specific for Proteus X 19 and X 2 strains is described. The secondary growth appearing after lysis of OX 19 cultures is devoid of the X-Antigen reacting with typhus sera. The phage is inactive against Proteus OXK.
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    Cellular and molecular life sciences 5 (1949), S. 447-448 
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    Notes: Summary Patulin was examined in regard to its effect upon the different white blood cells in white mice. There is a very noticeable influence on the lymphocyte count in the circulating blood, which was reduced by daily injections of patulin 0.1 mg i. p. from 8–15,000 to 1–3,000, while the granulocytes were scarcely affected. After the injections were stopped, the lymphocyte count increased again, and sometimes an increase of the granulocytes has been noticed. Often mice died from abscesses in different organs. Patulin seems to have an inhibitory effect on lymphatic tissues, like carbamates and nitrogen mustard. Whether there is a growth inhibitory effect on tumours is now an object of study.
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    Cellular and molecular life sciences 5 (1949), S. 476-478 
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    Notes: Zusammenfassung Der histochemische Nachweis der alkalischen Phosphatase beiAcanthochites fascicularis L. (Polyplacophora, Mollusca) erbringt den Beweis eines ausgedehnten Vorhandenseins des Ferments. Das Integument, das Bindegewebe und das Muskelgewebe enthalten keine oder nur Spuren von Desmophosphatase. Der Verdauungsapparat, insbesondere die Zuckerdrüse und der Mitteldarm sind dagegen reich an Phosphatase, desgleichen die Niere, die Spermatogonien und Spermatozyten, die Nukleolen der Ovozyten und das Epithel des Ovariums.
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    Cellular and molecular life sciences 5 (1949), S. 484-486 
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    Cellular and molecular life sciences 5 (1949), S. 487-487 
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    Cellular and molecular life sciences 5 (1949), S. 498-501 
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    Notes: Summary The historical dispute in whichGoethe opposedNewton's theory of colour can be referred to the discrepancy between the concepts of “light” as physical radiation and the concept of “light” as sensation. Language as well as natural inclination lead to the confusion and even identification of the two wholly different concepts, whereas the physical laws thatNewton treated and the psychophysical laws that chiefly interestedGoethe are not in agreement.Goethe rightly boasted of „having rescued the subjective colours“.
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    Cellular and molecular life sciences 5 (1949), S. 65-66 
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    Notes: Summary Some particularities of the diffraction spectra produced by the lattice of a tissue have been discussed. An account is given of the apparition of supplementary diffraction maxima when the light-beam forms an angle ≠ 90° with the lattice-plane.
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    Cellular and molecular life sciences 5 (1949), S. 161-162 
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    Notes: Summary The cobweb of geometrical spiders enables us to examine the effect of substances on instinct movements of animals. It seems possible to separate the different fundamental functions co-operating in the production of the cobweb by experiment. The effect of Pervitin, mentioned in this report, is characterized by a considerable disturbance of the regular arrangement of the concentric sticky threads.
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    Cellular and molecular life sciences 5 (1949), S. 167-167 
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    Notes: Zusammenfassung Im PilzInoloma traganum wurde ein neuer antibakterieller Stoff gefunden. Diese Substanz wirkt nur auf einige Arten von Mikrokokken und Pseudodiphtheriebazillen. Das neue Antibiotikum wurde aus einer Wasserlösung durch Adsorption an aktive Kohle konzentriert, mit konzentriertem Äthanol eluiert und durch Verdampfen der Lösung gewonnen. Für eine Maus ist der Stoff bei intravenöser Applikation nicht toxisch. Er könnte theoretisch beim Studium der systematischen Einordnung von Mikrokokken und Pseudodiphtheriebazillen bedeutsam sein.
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    Cellular and molecular life sciences 5 (1949), S. 172-172 
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    Cellular and molecular life sciences 5 (1949), S. 181-188 
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    Notes: Summary It is discussed which of the new rays of atomic physics (protons, neutrons, electrons) may be applicable for depth therapy. The range of protons is too short. Recent information from the U.S.A. shows that neutron therapy is not as successful as formerly supposed. Irradiation with fast electrons produced by the betatron may be superior to X-ray therapy. Some preliminary results of biological experiments withDrosophila eggs and ionization measurements are reported. The position of the maximum effect is some centimetres below the surface and not on it. Electrons have a definite range, so that the parts of the body beyond this range are not irradiated.
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