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  • Articles  (14)
  • Scanning electron microscopy  (14)
  • Springer  (14)
  • 2005-2009
  • 1980-1984  (14)
  • 1945-1949
  • 2006
  • 1980  (14)
  • 1949
  • Medicine  (14)
  • Geosciences
Collection
  • Articles  (14)
Source
Keywords
Publisher
  • Springer  (14)
Years
  • 2005-2009
  • 1980-1984  (14)
  • 1945-1949
Year
Topic
  • 1
    Electronic Resource
    Electronic Resource
    Scanning electron microscopy and X-ray diffraction studies of human bone oxalosis (1980)
    Springer
    Calcified tissue international 30 (1980), S. 109-119 
    Details
    ISSN: 1432-0827
    Keywords: Oxalosis ; Human bone ; Scanning electron microscopy ; X-ray diffraction ; Oxalate titration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary Postmortem scanning electron microscopy of human phalanges in a chronic uremic hemodialysis patient with hyperparathyroidism showed the presence of confluent abnormal rounded formations with a radial rosette-like crystalline pattern in the diaphysis as well as in the epiphyseal part of the bones. These fan-shaped configurations were found either as individual formations within bone trabeculae or as numerous aggregated crystalline deposits replacing large parts of the bone structure. The microdissected content of such large areas submitted to X-ray diffraction analysis revealed the predominant presence of calcium oxalate monohydrate or whewellite with some traces of hydroxyapatite. Oxalate titration analysis indicated the presence of 25% of oxalate, corresponding to 45% in weight of whewellite.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02408615
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  • 2
    Electronic Resource
    Electronic Resource
    The mature mesonephric nephron of the rabbit embryo (1980)
    Springer
    Cell & tissue research 209 (1980), S. 95-109 
    Details
    ISSN: 1432-0878
    Keywords: Mesonephric nephron ; Scanning electron microscopy ; Rabbitembryo ; Wolffian body
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The luminal surface ultrastructure of the mature mesonephric nephron in 18 day rabbit embryos was studied in order to classify the nephron segments and to compare them with their metanephric counterparts. The proximal tubule has two slightly different segments. Its brush-bordered cells, with lateral ridges and basal microvilli (revealed in disjoined cells) exhibit structural principles similar to those of metanephric cells. The short distal tubule, starting with an abrupt border, cannot be subdivided. Its surface differs from one specimen to the next; the various cellular patterns are regarded as different functional states rather than evidence of a true cellular heterogeneity. Cells with leaf-like meandering borders correspond to similar metanephric cells favoring a paracellular transport mechanism. The collecting tubule shares common features with the metanephric collecting duct in spite of its different origin. Among principal cells, clearly demarcated by marginal microvillous rows and studded with sparse apical microvilli, non-ciliated and strongly bulging intercalated cells occur in small numbers. The latter have exaggerated, sometimes branched microvilli, and occasional microplicae. In the Wolffian duct, which has no metanephric counterpart, the single cilia dominate the picture of a homogeneous cell population. Apical globular protrusions of the tubular epithelia, which have been depicted in almost every paper on the mesonephros, are all fixation artefacts that can only be avoided by properly perfusing the living embryo.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00219926
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  • 3
    Electronic Resource
    Electronic Resource
    A scanning electron microscope study of myoepithelial cells in exocrine glands (1980)
    Springer
    Cell & tissue research 209 (1980), S. 1-10 
    Details
    ISSN: 1432-0878
    Keywords: Myoepithelial cell ; Exocrine gland ; Scanning electron microscopy ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary By removing connective tissue components with enzymatic digestion followed by HCl-hydrolysis, myoepithelial cells (MECs) of the terminal portion in a variety of exocrine glands of the rat were examined with the scanning electron microscope. The profile of MECs varied considerably from gland to gland; MECs in the lactating mammary gland have a few long cytoplasmic processes in close contact with those of adjacent cells forming a continuous network around the terminal portion. Those of the exorbital lacrimal gland are stellate with many thin radiating processes with tapered ends that terminate freely. MECs in the sublingual gland are characterized by a number of broad and extensive cellular processes. MECs in the submandibular gland are similar in appearance to those of the exorbital lacrimal gland, but with more extensive cellular processes that form a more or less continuous network with those of the adjacent cells. No MECs were observed on the terminal portion of the parotid gland where the cells appear to be lodged on the intercalated duct. The relative surface area covered by MECs per terminal portion was also found to vary significantly, being 24% in the lactating mammary, 17% in the exorbital lacrimal, 48% in the sublingual, and 25% in the submandibular glands. The findings are discussed in relation to the physical properties of secretions in different glands.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00219918
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  • 4
    Electronic Resource
    Electronic Resource
    Irido-corneal angle of mammalian eyes: Comparative morphology of encapsulated corpuscles in odontocete cetaceans (1980)
    Springer
    Cell & tissue research 210 (1980), S. 501-515 
    Details
    ISSN: 1432-0878
    Keywords: Mechanoreceptors ; Eye ; Dolphins ; Whales ; Irido-corned angle ; Light microscopy ; Scanning electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A study of the structure and distribution of encapsulated corpuscles (corpuscles of Rochon-Duvigneaud) in the irido-corneal (anterior chamber) angle in 42 adult eyes from nine species of odontocete cetaceans representing each of the five families in this group indicates a diversity of form and function. The corpuscles occur as single features or multiple groupings with each species having a distinct pattern. No definite phylogenic or environmental patterns were seen although sometimes there were similarities among related genera. The neural structure of the angle suggests that these receptors probably respond to a variety of stimuli including intraocular pressure, vitreous displacements, external muscular force applied to the sclera and cornea, and changes in the volume of the massive iris/choroid vascular system.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00220206
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  • 5
    Electronic Resource
    Electronic Resource
    Lack of Fc receptors on osteoclasts (1980)
    Springer
    Cell & tissue research 212 (1980), S. 509-516 
    Details
    ISSN: 1432-0878
    Keywords: Osteoclasts ; Macrophages ; Fc receptors ; Scanning electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Fc and C3 receptors, which are characteristically present on macrophages, could not be demonstrated on osteoclasts maintained in situ on their normal substrates when assayed for by use of sheep red blood cells coated with immmoglobulin (Shapiro et al. 1979). The present study tested the hypotheses that Fc receptors are present only on the osteoclast surface adjacent to bone and that Fc receptors on osteoclasts can be uncovered by enzymes or stimulated to appear. Freeze-dried, inverted osteoclasts (and osteoblasts) obtained from the endocranium of newborn rats were tested for Fc receptors using the rosette assay and examined by scanning electron microscopy. No rosettes were observed on the surfaces of the osteoclasts that had been approximal to the bone. Bone specimens were cultured for 30 min at 37° C in control medium, or in medium with the addition of 10, 50 or 100 gmg/ml trypsin, 0.5 U/ml parathyroid extract (PTE), or 0.5 or 1U/ml parathyroid hormone 1–34 (PTH). Additionally, two week-old rats were injected intraperitoneally with PTE (1.5 U/g body weight or 1USP/g body weight) or with PTH (1U/g body weight) or with vehicle alone, 6 h before sacrifice. The specimens were assayed for Fc receptors and examined by scanning electron microscopy. Macrophages were always used as controls for the assay. No rosettes were present on osteoclasts subjected to any of these treatments. Accordingly, the hypotheses were not supported.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00236514
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  • 6
    Electronic Resource
    Electronic Resource
    Scanning electron microscopy of the cleft of the rat pituitary (1980)
    Springer
    Cell & tissue research 206 (1980), S. 139-143 
    Details
    ISSN: 1432-0878
    Keywords: Pituitary cleft ; Scanning electron microscopy ; Cell surface changes ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Scanning electron microscopy of the lining of the pituitary cleft was carried out in normal, lactating, castrated, adrenalectomized, and cyproterone-treated adult rats. Four cell types could be differentiated in the posterior wall in control and experimental animals: (1) cells with a smooth surface, (2) cells with microvilli located at the cellular borders, (3) ciliated cells, and (4) cells with evenly distributed microvilli. The anterior wall showed mainly cells with few microvilli located at their margins, and clusters of ciliated cells. In normal, and more frequently in experimental animals, the anterior wall showed shriveled cells, and variously sized cavities. Colloid appeared either as a network of finely granular material or as compact bodies adhering to the epithelial surface. These observations suggest that a compact component of the colloid is derived at least in part from degraded cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00233614
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  • 7
    Electronic Resource
    Electronic Resource
    Formation and morphology of Reissner's fibre in primates (1980)
    Springer
    Cell & tissue research 207 (1980), S. 43-53 
    Details
    ISSN: 1432-0878
    Keywords: Reissner's fibre ; Subcommissural organ ; Scanning electron microscopy ; Primates
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The subcommissural ependyma of the third ventricle, the rhomboid fossa, and the central canal of the spinal cord were examined in Tupaia, Saimiri, and Cebus by scanning electron microscopy. In all three species studied, fine filaments, granules, and amorphous masses were found at the surface of the subcommissural ependyma representing the original structures of the Reissner's fibre (RF). Bundles of filaments or net-like structures ultimately forming a compact secretory plate develop in the caudal part of the secretory zone. In Tupaia and Saimiri, the secretion collects in a groove-like depression in the centre of the dorsal ventricular wall. In Cebus two secretory pathways occur in the lateral sulci, which eventually merge together in the aqueduct. In the rhomboid fossa and the spinal cord, the RF is in close contact with the ependyma, and its surface is longitudinally grooved and covered with granular material and cells. The present scanning electron microscopic findings speak in favour of a merocrine type of secretion in the apical region of the ependymal cells. Ciliary movement and flow of cerebrospinal fluid are assumed to be the most important factors regulating the formation of the RF.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00239328
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  • 8
    Electronic Resource
    Electronic Resource
    Changes of the caecal villi during postnatal development in rats (1980)
    Springer
    Cell & tissue research 208 (1980), S. 253-259 
    Details
    ISSN: 1432-0878
    Keywords: Villus ; Caecum ; Postnatal development ; Rat ; Scanning electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The villi of the caecal mucosa in postnatal rats were studied using both scanning electron and light microscopy. On the day of birth, numerous villi of various sizes and shapes were present on the caecal mucosa. After the 5th day, the villi decreased very rapidly in length and in number. A strong constriction was observed at the basal region of the caecal villi. During postnatal days 5 ∼ 9 the villi probably separated and disappeared from the caecal mucosa. No villi were observed in rats that were over 10 days of age.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00234875
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  • 9
    Electronic Resource
    Electronic Resource
    Scanning electron microscopic observations of early stages of phagocytosis of E. coli by human neutrophils (1980)
    Springer
    Cell & tissue research 209 (1980), S. 65-70 
    Details
    ISSN: 1432-0878
    Keywords: Phagocytosis ; Human polymorphonuclear leukocytes ; Neutrophils ; E. coli ; Scanning electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Changes in surface morphology, as observed by scanning electron microscopy, appear rapidly when human polymorphonuclear neutrophils (PMN) are challenged with bacteria. Monolayers of PMN adhering to glass were incubated with opsonized E. coli from 5 sec to 10 min, and then fixed and prepared for SEM. As early as 5 sec after phagocytic challenge, E. coli are found in contact with PMN and in the process of engulfment into open cavities formed by lamellipodia. The shape of the mouth of the forming phagocytic vacuole is related to the orientation of bacteria during entry. Bacteria engulfed into early forming phagosomes are surrounded by a large open space between the bacteria and the phagosome wall. As phagocytosis proceeds, the space is reduced and the loose fit around the entering bacteria becomes tight. By 30 sec, bacteria may be completely internalized and by 1 min phagocytized E. coli are packed into bulging PMN. The observations reveal the variability and rapidity of the phagocytic response and confirm the presence of sensitive mechanisms for host defense by PMN.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00219923
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  • 10
    Electronic Resource
    Electronic Resource
    A three-dimensional study of the normal human placental villous core (1980)
    Springer
    Cell & tissue research 210 (1980), S. 235-247 
    Details
    ISSN: 1432-0878
    Keywords: Chorionic villi ; Macrophages ; Placenta (human) ; Scanning electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In order to study the three-dimensional ultrastructure of the Hofbauer cells, human placentae from the 6th to the 21 st week of gestation and also from the end of pregnancy were cryofractured and observed by scanning electron microscopy. Hofbauer cells were found in the villous core at all the gestation stages examined. Their surface morphology was characterized by lamellipodia, funnel-like structures, blebs and microplicae. This pleomorphic aspect was probably related to functional or environmental conditions. In addition, thin cytoplasmic processes connected the Hofbauer cells with each other and with the components of the villous stroma. Fractured Hofbauer cells revealed large vacuoles in the cytoplasm; the vacuoles were smaller in size both at the beginning and at the end of pregnancy. This study further attests to the macrophagic nature of these cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00237612
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