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  • Articles  (55)
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  • Articles  (55)
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  • Springer  (55)
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  • 2005-2009
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  • 1975-1979  (55)
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  • 1
    Electronic Resource
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    Springer
    Applied physics 16 (1978), S. 47-57 
    ISSN: 1432-0630
    Keywords: 32 ; 42.65
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract Analytical results have been obtained for some aspects of polyatomic molecular interaction with resonant ir laser radiation. The following effects are investigated by use of Schrödinger's equation solution for natural multilevel models: the role of dynamic Stark effect in the transient region from molecular low levels to the quasi-continuum of highly excited vibrational levels; broadening of multiphoton transitions because of relatively fast decay into the quasi-continuum; threshold excitation property of multilevel systems with random detunings. Possible applications to constructing models for numerical calculations dealing with the molecule excitation problem are discussed.
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  • 2
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    Applied physics 17 (1978), S. 151-154 
    ISSN: 1432-0630
    Keywords: 33 ; 42.65
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    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract Lines of theQ-branch of vibrational rotational spectra are distinguished fromP(R)-lines by light induced anisotropy in absorbing gases. We report, for the first time, on experiments using a linearly polarized saturation beam. A CO2-laser was employed to investigate the molecule SF6.
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  • 3
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    Applied physics 17 (1978), S. 199-201 
    ISSN: 1432-0630
    Keywords: 32 ; 42.65
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    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract Lamb-dips have been observed in the weak calcium intercombination line by monitoring resonance fluorescence in an atomic beam excited by light of a stabilized cw dye laser inside or outside the laser cavity. A highly sensitive detection scheme is described.
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  • 4
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    Applied physics 15 (1978), S. 185-190 
    ISSN: 1432-0630
    Keywords: 42.55
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    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract Electron energy loss studies of POPOP andp-terphenyl in the vapor phase at an incident electron energy of 25 eV have been performed. The spectra covering an energy loss between 0 and 9 eV revealed a considerable initial triplet population under electron impact excitation. The implications for electron-beam pumped vapor phase dye lasers are discussed.
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  • 5
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    Applied physics 15 (1978), S. 209-211 
    ISSN: 1432-0630
    Keywords: 42.65
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    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract Continuous coherent radiation in a gas observed in a spacially separated standing wave fields is reported for the first time. The radiation was observed in the center of lineF 2 (2) in methane at the interaction of atoms with two standing waves separated by the distance of 3.5 cm.
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  • 6
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    Applied physics 15 (1978), S. 229-232 
    ISSN: 1432-0630
    Keywords: 42.55
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    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract A new type of discharge tube for high-power argon-ion lasers constructed of a segmented metal-ceramic compound system is described. Up to date, the system has been operating for 1500 h without failure. The maximum cw output is 140 W, limited by the destruction of the mirrors.
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  • 7
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    Applied physics 15 (1978), S. 219-222 
    ISSN: 1432-0630
    Keywords: 42.65
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    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract The paper investigates the observation of coherent radiation in separated fields for the case of two-level atoms. The coherent radiation is due to polarization transfer at large distances. The Doppler phase angle is cancelled due to a phase jump at the nonlinear interaction of the fields with the atoms. A peak in intensity with a width inversely proportion to the flight time arises at the line center.
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  • 8
    ISSN: 1432-0630
    Keywords: 42.65 ; 32
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract Coherent radiation at 89.6 nm has been obtained through third-harmonic conversion of the 268.8 nm pulses in mercury vapors. Tunability of the fourth-harmonic frequency of the Nd: glass laser radiation to the 6s 1 S 0–8s 1 S 0 double quantum resonance of Hg atoms has been used for the resonant enhancement of the twelfth-harmonic output. Possibility to phase match the generated radiation and driving polarisation in pure vapors without employing of bufer gas has been demonstrated.
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  • 9
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    Applied physics 15 (1978), S. 439-450 
    ISSN: 1432-0630
    Keywords: 42.55 ; 42.65
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    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract A theoretical model for the ultrashort-pulse (USP) generation by lasers with saturable absorbers is presented. The gain medium is assumed to be a two-level system whereas the absorber is described by a four-level system which is characterized by a two-stage absorption process and the pertinent relaxation times. Laser dynamical equations are developed in the rate-equation approximation and boundary conditions appropriate for an unidirectional ring cavity are established. Evolution of USP is investigated for different combinations of parameter values appropriate for solid-state laser systems by computer simulation, employing a finite difference approximation for the dynamical equations. It is shown that USP output is attainable even if the cross-section of the excited-state absorption isgreater than that of the ground-state absorption and the laser is operating just above threshold. In fact, it is found that through the participation of a strong excited-state absorption the discrimination against satellite pulses is enhanced so that single-pulse output is more achievable. Furthermore, it is proposed that single picosecond pulses may be obtainable from relatively broad initial peaks by utilizing the high pulse-selection and pulse-shortening efficiency of the absorber due to the contribution of the excited-state absorption. The applicability of the present model to singlet-triplet crossing and photoisomer formation is also discussed.
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  • 10
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    Applied physics 16 (1978), S. 235-237 
    ISSN: 1432-0630
    Keywords: 32 ; 42.65
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract J-dependence of intensity-dependent polarization change of the elliptically polarized light in atomic resonance lines is studied both theoretically and experimentally.
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  • 11
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    Applied physics 16 (1978), S. 297-301 
    ISSN: 1432-0630
    Keywords: 42.55
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    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract A grating-tuned cw dye laser with an emission bandwidth of less than 1 GHz and output power in excess of 400 mW has been constructed. This was accomplished using a newly developed holographic grating with sinusoidal groove profile, which resulted in an enhanced spectral efficiency up to 94%. The low losses and the broad spectral working range (more than 1000 Å) which can be easily covered by simple rotation make these gratings an attractive single-stage tuning element for low-gain cw lasers.
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  • 12
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    Applied physics 16 (1978), S. 151-154 
    ISSN: 1432-0630
    Keywords: 42.65 ; 42.55 ; 33
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract We observed tunable UV radiation down to 175 nm by SRS of the output of a frequency-doubled dye laser in H2. The high power output of the 4th anti-Stokes to 5th Stokes line generated by the frequency doubled dye laser and the 8th anti-Stokes to 3rd Stokes line generated by the undoubled dye laser represents a broadly tunable coherent radiation source between 189 nm and 2064 nm.
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  • 13
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    Applied physics 17 (1978), S. 367-370 
    ISSN: 1432-0630
    Keywords: 42.55 ; 78.55
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract We report on room temperature quasi-cw laser action in K5Nd(MoO4)4 at a wavelength of 1.067 μm. The crystal was pumped with the 5140 Å line of an argon ion laser. Due to thermal effects actual cw lasing was not possible. Chopping the pump light with a low duty cycle of 1∶1000 leads to quasi-cw laser action and stable output power. We have determined the fundamental laser parameters: threshold pump powerA T=15 mW, slope efficiency η=22%, and internal lossesL=2.5%. In order to explain the measured relaxation oscillations, diffusion of the excited 4f-excitons is considered. According to a recently developed theory, a diffusion constant ofD=2×10−6cm2/s explains the observed results.
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  • 14
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    Applied physics 15 (1978), S. 191-196 
    ISSN: 1432-0630
    Keywords: 42.55
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract A guideline for the optimum design of linear wall-ablation lamps is presented with a simple empirical formula which describes the luminescent characteristics of lamps. The absolute luminescent efficiency of the wall-ablation lamp was measured. A high-energy dye laser with a quadruple-elliptical cylindrical cavity was constructed using these lamps, and an output energy of 23.3 J (11.6 MW) was obtained with an overall efficiency of 0.31 %. The energy-flow diagram of this laser system was computed with a rate-equation analysis, and the conversion efficiency from electrical input to laser output was determined. One can expect an overall efficiency of about 2 %.
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  • 15
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    Applied physics 15 (1978), S. 213-218 
    ISSN: 1432-0630
    Keywords: 42.65 ; 42.80 ; 32
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract The Wiener functional expansion method for the analysis of nonlinear systems is applied to identify and analyze both nonlinear and linear molecular systems by spectroscopic methods. As the sampling filter (monochromator) of any spectroscopic apparatus may be defined by a Weber-Hermite polynomial, an analysis of the refracted or scattered light by orthogonal polynomials is easily achieved. Time averaging obtains the Weber-Hermite coefficients which permit the characterization of the molecular system with respect to the polarization of the incident and scattered light. In the case of two series of measurements made with incident and emerging light polarized in different directions: the identification of the JonesM matrices for the molecular system irradiated is possible. In the case of three series of measurements made, for example, with incident and emerging light (a) circularly polarized corotating, (b) circularly polarized contrarotating, and (c) plane polarized perpendicular: the identification of the molecular system's McClain invariants related to the vibrational symmetry group for Raman inelastic light scattering is possible. The analysis presents a unified picture of elastic and inelastic light scattering and one-photon and two-photon processes. The apparatus described would detect those instances in molecular systems for which Beer's law does not apply.
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  • 16
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    Applied physics 17 (1978), S. 53-62 
    ISSN: 1432-0630
    Keywords: 42.10 ; 42.65
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract The coherent interaction of short optical pulses with a nonlinear active resonant medium is calculated. The rigorous and self-consistent solution of the coupled nonlinear Maxwell-Bloch equations including transverse and time-dependent phase variations predicts the onset on an on-resonance self-focusing that agrees very well with a previous perturbation treatment and with recent experiments in sodium and neon. The formation of this dynamic self-action effect is elucidated as being due to the combined effects of diffraction and the inertial response of the active media. Accuracy and computational economy are achieved simultaneously by redistributing the computational grid points according to the physical requirements of the problem. Evenly spaced computational grids are related to variable grids in physical space by a range of stretching and rezoning techniques including adaptive rezoning where the coordinate transformation is determined by the actual physical solution.
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  • 17
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    Applied physics 17 (1978), S. 73-77 
    ISSN: 1432-0630
    Keywords: 42.65 ; 52
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    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract The effect of self-focusing of a high-power light beam with an elliptical cross-section in a strongly ionised plasma has been evaluated. The mechanism of self-focusing considered is non-uniform heating of plasma by an electromagnetic wave having transverse variation of amplitude along its wave front. The heating causes redistribution of carriers which lead to a nonlinear relationship between the electron density and the electric vector and hence to a field-dependent effective dielectric constant. It is found that the beam gets focused at different focal points in different directions exhibiting the effect of astigmatism. There are several critical powers, below thex=0,y=0 planes; at higher powers one dimension focuses while the other defocusses, an oscillatory waveguide is formed in both dimensions. Above the highest critical power both dimensions self-focus, the dimensionless beam width parametersf 1 andf 2 forx andy focusing reach different minima corresponding to different self-focusing distances beyond which both dimensions continue diverging. The effect of energy loss through absorption from the beam has also been considered. It has been observed that absorption brings about a reduction in the extent of self-focusing and favours defocusing of the beam.
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  • 18
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    Applied physics 17 (1978), S. 181-187 
    ISSN: 1432-0630
    Keywords: 42.55
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract A complete theoretical model for optically pumped dye lasers is studied using the rate equation approach which includes excited singlet and triplet state absorption effects. Closed-form solutions under steady-state conditions reveal a novel nonlinear threshold phenomenon: partial reabsorption of the excited laser light can lead to a discontinuous onset of laser oscillation above laser threshold. Theoretical and experimental results for a transversely and longitudinally pumped rhodamine 6G laser are presented in terms of softmode and hardmode behavior at threshold.
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  • 19
    ISSN: 1432-0630
    Keywords: 32 ; 42.65
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    Notes: Abstract The paper presents the results obtained in investigating the lineshape of two-photon absorption in the field of two spacially separated standing waves at the 3S–4D transition of Na. Measurements were carried out by using a CW dye laser.
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  • 20
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    Applied physics 15 (1978), S. 99-102 
    ISSN: 1432-0630
    Keywords: 42.65 ; 78.20
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    Notes: Abstract Comparing the recombination light intensity produced in GaAs by a 1.06 μm pulse inducing two-photon absorption, with the intensity produced by a 0.53 μm pulse, we obtain directly the two-photon absorption cross-section. This method can be generalized to other materials.
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  • 21
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    Applied physics 17 (1978), S. 257-267 
    ISSN: 1432-0630
    Keywords: 42.55 ; 32.60
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    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract The effect of transversally applied magnetic fields on the polarization behavior of a single-mode gas laser is theoretically investigated, stressing the importance of the additivity of respective contributions influencing the time evolution of polarization parameters. By adding a new element to the existing theory, namely the contribution of magnetic-field-free regions in the active medium, and by considering the relatively large effects that small cavity anisotropies can cause, several apparent discrepancies between experiments and existing theory are resolved.
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  • 22
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    Applied physics 15 (1978), S. 59-63 
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    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract Feedback controlled self-excitation of optical pulses of nanosecond duration has been observed to occur rather reliably in a CW dye ring laser, oscillating in a traveling mode. These observations are described analytically by means of a direct time domain approach. It is shown that a steady-state Gaussian pulse whose time duration determined from the self consistency condition in terms of system characteristics describes accurately the observed pulse behaviors.
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  • 23
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    Applied physics 15 (1978), S. 53-57 
    ISSN: 1432-0630
    Keywords: 71.20 ; 42.65
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    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract In this paper we report on measurements of spin-flip-Raman gain inn-InSb as a function of the magnetic field. The measurements were carried out at temperatures of 1.8 K and 4.2 K and at a carrier concentration of 1.35×1015 cm−3. The Raman cross sections obtained from these results, e.g. 1.25×10−20 cm2/sr at a magnetic field of 10 kG and a pump frequency of 1884.35 cm−1, agree very well with those theoretically predicted by Wherrett and Wolland. Furthermore, these measurements yield line shapes and linewidths of the spontaneous scattering (100–1500 MHz) and allow the determination of the effectiveg-value with an accuracy known from ESR-investigations. These results are discussed in terms of already published theoretical investigations.
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  • 24
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    Applied physics 15 (1978), S. 265-270 
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    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract For a homogeneously broadened two-level ring laser model the stability of the cw laser oscillation in the presence of a passive medium is investigated. The passive material is treated similar to the active material, but without an external pumping mechanism. Stable and unstable regions are shown for various parameters. The stability region of the cw solution becomes extremly small for certain parameters.
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  • 25
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    Applied physics 15 (1978), S. 319-322 
    ISSN: 1432-0630
    Keywords: 42.65
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    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract This paper reports on an experimental observation of coherent radiation after interaction with two standing-wave pulses having the time delayT in an absorbing rarefied gas. The radiation appears at the time 2T, 3T,... after the first pulse. The coherent radiation arises due to spacial transfer of polarization and to formation of a spacial polarization harmonic owing to a phase jump after two-photon interaction with the field of the second pulse. We observed intensity oscillations with frequency tuning of the exciting laser, varying the time delay between pulses.
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  • 26
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    Applied physics 16 (1978), S. 155-158 
    ISSN: 1432-0630
    Keywords: 42.55 ; 78.60 ; 85.60
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract Dynamic characteristics of laser diodes are numerically calculated on multi-mode rate equations, including spatial relaxing interaction causing mode competitions or jumps. It is revealed that the interaction effect is classified into three cases [“independent (or inhomogeneous)”, “competition (or intermediate)”, and “monopoly (or ideally homogeneous)”], that an optimum relaxing coefficient value exists to minimize relaxation oscillations in rise and decay transients, and that the optimum value is related to the relaxation period. Calculated results are discussed in comparison with the experimental results.
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  • 27
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    Applied physics 17 (1978), S. 423-424 
    ISSN: 1432-0630
    Keywords: 42.65 ; 42.80
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    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract An optical arrangement for studying two-photon absorption in liquids is presented. The main feature of this new arrangement is fuller utilization of fluorescence light. This makes possible application of very low power cw laser in such experiments.
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  • 28
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    Applied physics 17 (1978), S. 303-307 
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    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract A new and simple technique for measuring the effective diameter of a laser beam used in material processing is described. The time for the temperature of a spot heated by the laser beam to rise to 90% of equilibrium is compared with that predicted theoretically for a Gaussian TEM00 laser beam. A Gaussian beam diameter equivalent is thus deduced. This calculated diameter is of particular relevance to applications where the laser is used as a heat source.
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  • 29
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    Applied physics 17 (1978), S. 393-400 
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    Notes: Abstract Stimulated Raman scattering by polaritons when employing a nonlinear crystal inside the cavity of the laser with Q-switching and locked to fundamental frequency was theoretically studied. The equations were formulated describing the dynamics of the fundamental, Stokes and polariton waves under the assumption of instant Q-switching and uniformity of radiation except in the longitudinal direction. Numerical solutions of the equations were found and analysed. We demonstrate the influence, on generation characteristics, of the most important parameters, such as the effective nonlinearity, the initial level of population inversion, the feedback of the Stokes wave and the group mismatch between a Stokes and a pump wave. Optimum generation conditions are determined. Possibilities of the method are discussed for the generation of a) giant pulses which are controllable with regard to carrier frequency and duration but are unachievable with other methods, b) Stokes pulses of very small duration and high energy having sharp trailing edges, and c) analogous polariton pulses in the middle and far infrared range. The generation using a number of polariton branches in LiNbO3 was considered as an example. Calculations are in qualitative agreement with experimental data.
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  • 30
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    Applied physics 17 (1978), S. 211-232 
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    Keywords: 42.55 ; 42.65 ; 42.80
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    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract Physical principles, new ways and means of creation, schemes, characteristics and features of efficient high-power tunable pulse Raman lasers, operating in the near and middle ir are reviewed. The paper includes: tunable dye and Nd lasers as pump sources; promising active media and their optimal excitation methods; optical systems for producing spatially homogeneous pumping; the physics of Raman oscillators and their practical schemes, efficient high pulse energy liquid N2 and compressed H2 Raman oscillators, covering several bands in the range between 1.4 and 9.2 μm; the physics and construction of efficient tunable Raman amplifiers-convertors, amplifying in the saturation regime of spontaneously scattered or beforehand produced and collimated external Stokes signals, obtained in the spectral range between 0.83 μm and 18 μm. Raman laser using a, so-called, broadband pump where the linewidth of pumping light is broader than the spontaneous scattering linewidth, are also discussed. Features of both amplification and oscillation regimes of such broadband pumped Raman lasers are reported, and conditions for the efficient frequency conversion are determined.
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  • 31
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    Notes: Abstract Design and operating caracteristics of high pulse repetition rate NH3 laser producing up to 20 W of average output power are described. The NH3 laser, operating in the 12–13 μm region was optically pumped with a high pulse repetition rate TEA CO2 laser. Dependences of the NH3 laser output on the pump energy, ammonia and buffer gas pressures and pulse repetition rate have been studied. The conversion efficiency of up to 16% has been received.
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  • 32
    ISSN: 1432-0630
    Keywords: 35 ; 42.55 ; 42.68
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    Notes: Abstract We report the first accurate measurements of the transition strengths and linewidths of the 00o2 9.4 μm sequence lines of CO2. This sequence band is found to be very similar to the regular 00o1 laser band, except for an experimentally determined factor of 1.89±0.12 increase in transition strength (if the CO2 molecule is treated as a simple harmonic oscillator the theoretical increase in transition strength is 2.0). The laser absorption spectrometer used for these measurements can detect absorption coefficients in CO2 of ≈10−7 m−1, and we discuss the feasibility of using this sensitivity to monitor rare isotopic forms of CO2.
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  • 33
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    Planta 139 (1978), S. 155-158 
    ISSN: 1432-2048
    Keywords: Avena ; Cell-wall regeneration ; Electrical potential ; Nicotiana ; Protoplast culture ; Turgor pressure ; Zea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Four independent kinds of observations indicate that the cell wall regenerated by oat (Avena sativa L.) and corn (Zea mays L.) protoplasts in culture is less well developed than that regenerated by tobacco (Nicotiana tabacum L.) protoplasts. Following wall regeneration the cereal protoplasts remained susceptible to osmotic shock upon transfer to water, showed great enlargement, stained poorly with calcofluor white, and maintained a positive internal electrical potential. The development of a negative membrane potential by tobacco protoplasts in culture often occurred simultaneously with the onset of cell division. Since division was observed only in protoplasts which had regenerated good cell walls and had re-established negative membrane potentials it is suggested that culture conditions which favor these two processes should improve protoplast viability.
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  • 34
    ISSN: 1432-2048
    Keywords: Auxin binding ; Coleoptiles ; Multiple receptors ; Tonoplast ; Zea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In-vitro binding of labeled auxins to sedimentable particles was tested in subcellular fractions from homogenates of maize (Zea mays L.) coleoptiles. The material was fractionated by differential centrifugation or on sucrose density gradients. It was confirmed that the major saturable binding activity (site I) for 1-naphthyl[1-14C]acetic acid is associated with vesicles derived from the endoplasmatic reticulum. A second type of specific auxin binding (site II) could be distinguished by several criteria, e.g. by the low affinity towards phenylacetic acid. The particles carrying site II could be clearly separated from markers of the endoplasmatic reticulum, the plasmalemma, the mitochondria and the nuclei, while their density as well as sedimentation velocity correlated with particle-bound acid phosphatase, indicating a localization at the tonoplast. In contrast to site I, binding at site II was hardly affected by a supernatant factor and by sulfhydryl groups. However, the specificity pattern of site II towards auxins and auxin analogs was very similar to that of site I tested in the presence of supernatant factor. The existence of a third auxin receptor localized in plasma membrane-rich gradient fractions was indicated by a preferential in-vitro binding of 2,4-dichlorophenoxyacetic acid.
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  • 35
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    Planta 140 (1978), S. 201-211 
    ISSN: 1432-2048
    Keywords: Auxin ; Cell elongation ; Coleoptiles ; Fusicoccin ; Zea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract To analyze early effects of auxin application, an apparatus was developed which continuously and simultaneously registered the curvature of 10 individual maize (Zea mays L.) coleoptiles. Resolution was less than 5 μm over a range of ±0.5 mm. The data were evaluated and plotted via paper tape and Hewlett-Packard-computer. Unilateral application of 3×10-5 M indoleacetic acid (IAA) resulted in a transient inhibition of growth on the side of application for ca. 10 min (Phase I), followed by a strong stimulation (Phase II). The phytotoxin fusicoccin (FC) caused an immediate stimulation of elongation. The initial negative reaction of Phase I is auxin-specific. Only active auxins such as IAA and 1-naphtaleneacetic acid produced this initial inhibition; chemical analogs-inhibitory or neutral in long-term growth tests, e.g. phenylacetic acid-did not show any significant effects on Phase I. When the coleoptiles were symmetrically preloaded with different levels of auxin, only a large step-up of subsequent unilateral auxin application resulted in a negative phase I; a small step-up led to an immediate positive reaction. The results are discussed in context with the parallel kinetics for various other auxin-induced reactions of coleoptile cells which have already been published.
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  • 36
    ISSN: 1432-2048
    Keywords: Chlorophyll (fluorescence) ; Fluorescence (chlorophyll) ; Lincomycin ; Zea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Light-induced fluorescence decay was examined during the greening of control and lincomycintreated maize (Zea mays L.) leaves. Assuming that this decay to a first approximation is the result of two parallel first-order reactions, the fluorescence induction curves were linearized on the logarithm plot and the parameters were determined. The variable fluorescence increased, and the parameters of the two linear sections of the fluorescence decay—that is, the kinetics of the induction curves—changed during the greening of the control leaves. Lincomycin treatment caused some chlorophyll deficiency and the lowering of the chlorophyll a/b ratio, changed the fluorescence emission spectra and the effect of Mg2+ on the regulation of the excitation energy distribution. The structure of the thylakoids and the kinetics of the fluorescence decay were also changed in the treated leaves. The possible relationship between the change of the kinetics of the fluorescence decay and the change of spillover during greening and after lincomycin treatment is discussed.
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  • 37
    ISSN: 1432-2048
    Keywords: Cell division ; Cell wall ; Golgi apparatus ; Secretory vesicles ; Zea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Epidermal cells of maize roots were studied to determine the distribution of Golgi apparatus-derived secretory vesicles in various stages of cell division. The following conclusions were reached: 1) The pattern of Golgi apparatus secretion varies with the cell cycle. 2) Large numbers of secretory vesicles are incorporated into the cell plate. 3) Secretory vesicles from the Golgi apparatus are incorporated primarily in walls undergoing expansion. 4) Secretory vesicles are smaller during mitosis and the first part of cytokinesis than they are during interphase. 5) Secretory vesicles account for at least 12–23% of cell-plate plasma membrane and an estimated 25% of cell-plate volume.
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  • 38
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    Planta 138 (1978), S. 119-122 
    ISSN: 1432-2048
    Keywords: Glycosidases ; Root ; Zea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Four glycosidases were analyzed in 10 mm apical segments prepared from growing roots (15 mm) of Zea mays L. The pH optima were found to be 5.8 for β-glucosidase, 4.4 for β-galactosidase, 6.4 for α-glucosidase and 6.0 for α-galactosidase. The β-glucosidase showed 4-fold higher activity than the β-galactosidase. The distribution of the β-glucosidase activity was signifcantly different from that of the β-galactosidase, α-glucosidase and α-galactosidase.
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  • 39
    ISSN: 1432-2048
    Keywords: Auxin ; Fusicoccin ; Glucose transport ; Proton flux ; Roots ; Zea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Auxin and fusicoccin (FC) stimulate the active uptake of 3-O-methyl glucose (3-O-MG) in those materials in which they have been shown to activate an electrogenic proton extrusion (Pisum sativum L. stems, Zea mays L. coleoptiles and roots). In maize roots the curve relating 3-O-MG influx to external concentrations indicated that the values of the apparent Km increase in the 3-O-MG concentration range between 2×10-5 mol l-1 and 2×10-2 mol l-1. FC did not alter the Km values and its stimulating effect was nearly constant at all 3-O-MG concentrations tested. Basal and FC-induced uptake of 3-O-MG appeared associated with a transient proton influx suggesting that also in maize roots a sugar-proton contransport occurs. Diethyl stilbestrol, which inhibits proton extrusion, inhibited also basal and FC-induced 3-O-MG uptake. The data support the view that the stimulation by FC of 3-O-MG uptake is closely related to that of proton extrusion. The stimulation by FC of 3-O-MG uptake cannot be replaced by increasing extracellular proton concentration, nor may be explained only by the FC-induced hyperpolarization of transmembrane potential difference. The hypothesis is proposed that the effect of FC on 3-O-MG uptake depends on an increase of cytoplasmic pH, following the activation of the proton extruding system.
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  • 40
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    Planta 141 (1978), S. 179-181 
    ISSN: 1432-2048
    Keywords: Auxin ; Maize ; Root tip ; Zea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract [5-3H]Indol-3yl-acetic acid (IAA) applied to the shoot apices of intact 6-day-old maize (Zea mays L.) plants moved into the primary root and accumulated at the root apex. IAA from the shoot could partially satisfy the requirement of the primary root for IAA for growth.
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  • 41
    ISSN: 1432-2048
    Keywords: Antigen spectra ; Embryo (proteins) ; γ-Plantlets ; Germination (seeds) ; Globulins ; Proteins (storage) ; Seedling growth ; Storage proteins ; Zea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Normal and γ-irradiated caryopses of Zea mays L. were germinated, and the degradation of embryonal antigens (EA) was followed in the endosperms, scutella and embryonic axes of the seedlings, using double immunodiffusion, immunoelectrophoresis and quantitative immunoprecipitation. The predominant transient EA were presumed to be storage proteins related to the reserve globulins of dicotyledonous seeds. Therefore globulins were isolated from maize scutella, purified by (NH4)2SO4 fractionation and isoelectric precipitation, and the molecular weights of the polypeptide units were estimated by discontinuous sodium-dodecyl-sulphate slab electrophoresis. The globulins were found to be identical with the predominant EA and amounted to about 40% of the protein nitrogen in the embryos of mature, non-germinated caryopses. The presumed reserve function of the globulins and the characteristic time course of their degradation in embryonic axes and scutella of maize seedlings are discussed in relation to the two-step pattern of mobilization of nitrogen reserves in germinating cereal caryopses.
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  • 42
    ISSN: 1432-2048
    Keywords: Chlorophyll mutants ; Chloroplasts (development) ; Etioplasts ; Prolamellar body ; Zea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Four mutants of maize (Zea mays L.) defective in chlorophyll biosynthesis have been analyzed with regard to the sites of their lesions and their effects on chloroplast development. Two yellow mutants, which accumulate no detectable porphyrin precursors when grown in darkness, are defective in the conversion of protoporphyrin IX to magnesium protoporphyrin. Etioplasts of these mutants may develop elaborate lamellar membrane systems, but prolamellar bodies are never observed. Two mutants, which are necrotic when grown under illumination, develop normal (non-necrotic) leaf tissue in the dark and accumulate a small amount of magnesium protoporphyrin monomethyl ester, corresponding approximately to the amount of protochlorophyllide accumulated by normal plants. The etioplasts of these mutants contain noncrystalline bodies. The implications of these observations with respect to chloroplast development are discussed.
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  • 43
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    Planta 140 (1978), S. 31-35 
    ISSN: 1432-2048
    Keywords: Auxin ; Coleoptiles ; Electrical Potential ; Zea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Following asymmetric application of indoleacetic acid to maize (Zea mays L.) coleoptiles the early time course of changes in lateral electrical potential was externally monitored with static-drop electrodes. First, an early negative potential change of ca.-1 mV was measured at the surface on the side of a strong auxin application. This negative auxin effect ended after ca. 15 min and was followed by a strong and lasting auxin stimulation of a positive lateral potential up to +12 mV at the auxin-treated side. The initial auxin effect appeared to depend on the size of the step-up in auxin concentration.
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  • 44
    ISSN: 1432-2048
    Keywords: Aminopeptidases ; Carboxypeptidases ; Endopeptidases ; Germination (seeds) ; Proteases ; Seedling development ; Zea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Profiles of pH dependence and activities of live proteolytic enzymes, amino- and carboxypeptidase and endopeptidases active at pH 3.8, 5.4 and 7.5, with casein as substrate, were determined in crude extracts from the various organs of corn seedlings during germination and early development (30°C, dark, 8 d). With respect to the endopeptidases, caseolytic activities at pH 3.8, 5.4 and 7.5 in extracts from endosperm increased concurrently with loss of endosperm N during germination; however, the relative amounts of the pH 7.5 activity were very small. In scutellum extracts, caseolytic activities at both pH 5.4 and 7.5 increased during the initial stages of development but only the increase at pH 5.4 was concurrent with loss of scutellar N. In shoot extracts, caseolytic activities at pH 5.4 and 7.5 were very low and remained relatively constant. There was a progressive increase in shoot N with time. In root extracts, caseolytic activities at pH 5.4 and 7.5 were higher (3-fold) than in shoot extracts. The activity at pH 5.4 remained constant while the activity at pH 7.5 increased during germination. The rate of accumulation of N by the root was low after day 5. The pattern and ratio but not the amounts of the pH 5.4 and 7.5 caseolytic activities of the root were similar to those observed in senescing leaves of field-grown corn. Addition of mercaptoethanol increased (several-fold) the caseolytic activities at pH 3.8 and 5.4, especially the latter, but not the pH 7.5 activity in endosperm extracts and increased the pH 5.4 activity in extracts from scutellum (30%) and roots (30%) while the effect in shoot extracts was negligible. Carboxypeptidase activity was relatively low in young tissue (root tip, 3-d-old shoots) and increased with development of the various organs except the roots (whole) where the activity remained relatively constant. The increases in carboxypeptidase activities were concurrent with decreases in N from endosperm and scutellum; this result indicates that this enzyme in these tissues may be involved (cooperatively with endopeptidases) in the mobilization of reserve protein. Of all the enzymes tested, only carboxypeptidase activity was markedly (in excess of 50%) inhibited by phenylmethylsulfonylfluoride. Only aminopeptidase activity was found in appreciable amounts in endosperm and scutellum of dry kernels. Aminopeptidase activity was highest in organs with high metabolic activity (scutella, shoot, root tips) and decreased in plant parts undergoing rapid loss of nitrogen (endosperm, senescing leaves).
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  • 45
    ISSN: 1432-2048
    Keywords: Nuclei ; RNA polymerase ; RNA synthesis (in vitro) ; Zea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Nuclei were isolated from the shoots of Zea mays and assayed for endogenous RNA polymerase activity in vitro. Maximum incorporation from radioactive precursors (70 pmol [3H]uridine 5′ monophosphate/100 μg DNA) was reached after incubation for 1 h at 25°C. The RNA product, analysed by polyacrylamide gel electrophoresis, was polydisperse in size with an upper limit of 2x106 daltons. Discrete peaks of rRNA were not detected, probably because of endogenous ribonuclease activity. The inclusion of α-amanitin (4 μg/ml) in the incubation reduced the total incorporation by approximately 40% but did not significantly alter the size of the RNA product. Although 40% of the total activity could be attributed to RNA polymerase II, [3H]RNA synthesised in vitro was found not to contain long sequences of poly (A).
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  • 46
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    Planta 142 (1978), S. 269-274 
    ISSN: 1432-2048
    Keywords: Carboxylases ; Leaf development ; Photosynthesis (C4) ; Zea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The activities of several enzymes, including ribulose-1,5-diphosphate (RuDP) carboxylase (EC 4.1.1.39) and phosphoenolpyruvate (PEP) carboxylase (EC 4.1.1.31) were measured as a function of leaf age in Z. mays. Mature leaf tissue had a RuDP-carboxylase activity of 296.7 μmol CO2 g-1 fresh weight h-1 and a PEP-carboxylase activity of 660.6 μmol CO2 g-1 fresh weight h-1. In young corn leaves the activity of the two enzymes was 11 and 29%, respectively, of the mature leaves. In senescent leaf tissue, RuDP carboxylase activity declined more rapidly than that of any of the other enzymes assayed. On a relative basis the activities of NADP malic enzyme (EC 1.1.1.40), aspartate (EC 2.6.1.1) and alanine aminotransferase (EC 2.6.1.2), and NAD malate dehydrogenase (EC 1.1.1.37) exceeded those of both PEP and RuDP carboxylase in young and senescent leaf tissue. Pulse-chase labeling experiments with mature and senescent leaf tissue show that the predominant C4 acid differs between the two leaf ages. Labeling of alanine in senescent tissue never exceeded 4% of the total 14C remaining during the chase period, while in mature leaf tissue alanine accounted for 20% of the total after 60 s in 12CO2. The activity of RuDP carboxylase during leaf ontogeny in Z. mays parallels the development of the activity of this enzyme in C3 plants.
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  • 47
    ISSN: 1432-2048
    Keywords: Auxin ; Benzoxazolinones ; Receptors ; Supernatant factor ; Zea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A naturally-occurring material termed ‘supernatant factor’ [Ray, P.R., Dohrmann, U., Hertel, R.: Plant Physiol. 59. 357–364 (1977)], which has the property of modifying the binding affinity of auxins to receptor sites, has been isolated from corn (Zea mays L.) and characterised as a mixture of 6-methoxy-2-benzoxazolinone (MBOA) and 6,7-dimethoxy-2-benzoxazolinone (DMBOA). DMBOA is about 50 times more active than MBOA in inhibiting binding of the auxin 1-naphthylacetic acid to membrane-bound or solubilised receptors. The activity of these compounds and the parent analogue in inhibiting auxin binding is correlated with their ability to inhibit auxin-induced growth.
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  • 48
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    Planta 142 (1978), S. 275-279 
    ISSN: 1432-2048
    Keywords: Geotropism ; Light and geotropism ; Root geotropism ; Zea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The induction by light of geotropic responsiveness in the primary roots of Zea mays L. (cv. Golden Cross Bantam 70) was found to be governed by the “all-or-none law”. The response was induced by light energies above a threshold value, but the maximal curvature of geo-stimulated roots was constand irrespective of the light energy above that threshold. The action spectrum for this light effect showed a large peak at 650, a small peak at 410, and a shoulder at 663 nm. The effect of red light was not reversed by far-red light. Thus, the geotropic response in Zea roots may not be controlled by phytochrome.
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  • 49
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    Planta 144 (1978), S. 109-110 
    ISSN: 1432-2048
    Keywords: Growth (roots) ; Light and root growth ; Root cap ; Root growth ; Zea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A method using optical microfibers permitted localized exposure of the cap or the elongating part of growing maize (Zea mays L.) roots to white light. When the cap was illuminated, a strong and very rapid inhibition of the elongation rate of the roots was found. When the light microbeam was directed at the elongating region, the roots continued to grow at the same rate as before the illumination.
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  • 50
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    Planta 138 (1978), S. 279-294 
    ISSN: 1432-2048
    Keywords: Apoplast ; Companion cell-sieve tube complex ; Leaf structure ; Phloem loading ; Sieve tubes ; Symplast ; Vascular bundle ; Zea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Small and intermediate (longitudinal) vascular bundles of the Zea mays leaf are surrounded by chlorenchymatous bundle sheaths and consist of one or two vessels, variable numbers of vascular parenchyma cells, and two or more sieve tubes some of which are associated with companion cells. Sieve tubes not associated with companion cells have relatively thick walls and commonly are in direct contact with the vessels. The thick-walled sieve tubes have abundant cytoplasmic connections with contiguous vascular parenchyma cells; in contrast, connections between vascular parenchyma cells and thin-walled sieve tubes are rare. Connections are abundant, however, between the thin-walled sieve tubes and their companion cells; the latter have few connections with the vascular parenchyma cells. Plasmolytic studies on leaves of plants taken directly from lighted growth chambers gave osmotic potential values of about-18 bars for the companion cells and thin-walled sieve tubes (the companion cell-sieve tube complexes) and about-11 bars for the vascular parenchyma cells. Judging from the distribution of connections between various cell types of the vascular bundles and from the osmotic potential values of those cell types, it appears that sugar is actively accumulated from the apoplast by the companion cell-sieve tube complex, probably across the plasmalemma of the companion cell. The thick-walled sieve tubes, with their close spatial association with the vessels and possession of plasmalemma tubules, may play a role in retrieval of solutes entering the leaf apoplast in the transpiration stream. The transverse veins have chlorenchymatous bundle sheaths and commonly contain a single vessel and sieve tube. Parenchymatic elements may or may not be present. Like the thick-walled sieve tubes of the longitudinal bundles, the sieve tubes of the transverse veins have plasmalemma tubules, indicating that they too may play a role in retrieval of solutes entering the leaf apoplast in the transpiration stream.
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  • 51
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    Planta 139 (1978), S. 203-208 
    ISSN: 1432-2048
    Keywords: Auxin (bound) ; Auxin oxidation ; IAA oxidase ; Peroxidase ; Pisum ; Zea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Indole-3-acetic acid (IAA) was oxidized by horseradish peroxidase, but ester and amide conjugates of IAA were not degraded. Addition of indoleacetyl-myo-inositol, indoleacetyl-L-aspartate, indoleacetylglycine, indoleacetyl-L-alanine, indoleacetyl-D-alanine, or indoleacetyl-β-alanine did not affect the rate of oxidation of IAA by horseradish peroxidase. Peroxidase preparations from Pisum sativum L. and Zea mays L. behaved similarly in that they rapidly oxidized IAA, but not conjugates found in the plant from which the peroxidase was prepared. These results indicate that conjugation could affect the stability of IAA in vivo.
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  • 52
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    Planta 140 (1978), S. 1-6 
    ISSN: 1432-2048
    Keywords: Leaf temperature ; Oxygen (photosynthesis) ; Photosynthesis ; Temperature (photosynthesis) ; Triticum ; Zea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The effect of leaf temperature, O2 and calculated O2/CO2 solubility ratio in the leaf on the quantum yield of photosynthesis was studied for the C4 species, Zea mays L., and the C3 species, Triticum aestivum L. Over a range of leaf temperatures of 16 to 35° C, the quantum yield of Z. mays was relatively constant and was similar under 1.5 and 21% O2, being ca. 0.059 mol CO2 mol-1 quanta absorbed. Under 1.5% O2 and atmospheric levels of CO2, the quantum yield of T. aestivum was relatively constant (0.083 mol CO2 mol-1 quanta absorbed) at leaf temperatures from 15 to 35° C. Atmospheric levels of O2 (21%) reduced the quantum yield of photosynthesis in T. aestivum and as leaf temperature increased, the quantum yield decreased from 0.062 at 15°C to 0.046 mol CO2 mol-1 quanta absorbed at 35°C. Increasing temperature decreases the solubility of CO2 relatively more than the solubility of O2, resulting in an increased solubility ratio of O2/CO2. Experimentally manipulating the atmospheric levels of O2 or CO2 to maintain a near-constant solubility ratio of O2/CO2 at varying leaf temperatures largely prevented the temperature-dependent decrease in quantum yield in t. aestivum. Thus, the decrease in quantum yield with increasing leaf temperature in C3 species may be largely caused by a temperaturedependent change in the solubility ratio of O2/CO2.
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  • 53
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    Planta 139 (1978), S. 43-45 
    ISSN: 1432-2048
    Keywords: Avena ; Cell elongation ; Fusicoccin ; Malate synthesis ; PEP carboxylase ; pH stat ; Zea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The phytotoxin fusicoccin (FC) causes rapid synthesis of malate in coleoptile tissues, presumably via phosphoenolpyruvate (PEP) carboxylase coupled with malate dehydrogenase. The possibility that FC directly affects PEP carboxylase in Avena sativa L. and Zea mays L. coleoptiles was studied and rejected. The activity of this enzyme is unaffected by FC whether FC is added in vitro or a pretreatment to the live material. FC does not change the sensitivity of the enzyme to bicarbonate or malate. The activity of FC, instead, appears to be indirect. The pH sensitivity of PEP carboxylase is such that its activity, and thus the rate of malate synthesis, may be enhanced by an increase in cytoplasmic pH accompanying FC-induced H+ excretion. Since the enzyme is also particularily sensitive to bicarbonate levels, malate synthesis may also be enhanced by FC-induced uptake or generation of CO2.
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  • 54
    ISSN: 1432-2048
    Keywords: Abaxial ; Adaxial ; Carbon dioxide ; Light (blue) ; Stomatal conductance ; Zea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Attached leaves of Zea mays were illuminated with monochromatic light, with either the upper or the lower epidermis facing the light source. The mesophyll absorbed between 99.5 and 99.6% of the red or blue light used. An inversion of the light direction therefore caused a 200- to 250-fold change in the quantum flux into each epidermis. This variation in quantum flux did not affect stomatal conductance. Stomatal conductance was however correlated with intercellular CO2 concentration, ci, and the relationship between stomatal conductance and ci appeared also to remain the same if changes in ci were brought about by changes in atmospheric CO2 concentration instead of light. A close inspection of the data showed that stomata of the upper (adaxial) epidermis exhibited a small increase in conductance (〈0.1 cm s-1) in response to blue light that was superimposed on the dominating response to ci.
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  • 55
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    Theoretical and applied genetics 53 (1978), S. 43-46 
    ISSN: 1432-2242
    Keywords: Direct ovule pollination in vitro ; Embryo and endosperm development in vitro ; Hyperplastic nucellar growth ; In vitro pollination ; Sorghum ; Zea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Sorghum (Sorghum bicolor) pollen tubes penetrated and grew in corn (Zea mays) styles. The limited length of the sorghum pollen tubes (3–5 mm) and the absence of stigmatic hairs on the basal (5–10 mm) section of the corn styles prevented effective pollination in vivo and in vitro. Normal fertilisation occurred after in vitro pollination of exposed corn ovules with either corn or teosinte (Zea mexicana) pollen. Six per cent of corn ovules pollinated directly with sorghum pollen responded by rapid, massive growth of nucellar tissue.
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