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  • Articles  (118)
  • Electron microscopy  (70)
  • Immunocytochemistry  (49)
  • Springer  (118)
  • American Institute of Physics
  • International Union of Crystallography (IUCr)
  • Oxford University Press
  • 1985-1989  (44)
  • 1975-1979  (74)
  • 1945-1949
  • 2005
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  • Medicine  (118)
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  • Articles  (118)
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  • Springer  (118)
  • American Institute of Physics
  • International Union of Crystallography (IUCr)
  • Oxford University Press
  • Wiley-Blackwell  (1)
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  • 1985-1989  (44)
  • 1975-1979  (74)
  • 1945-1949
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  • 1
    Electronic Resource
    Electronic Resource
    The morphology of bone mineral crystals (1978)
    Springer
    Calcified tissue international 25 (1978), S. 217-222 
    Details
    ISSN: 1432-0827
    Keywords: Bone mineral ; Electron microscopy ; X-ray diffraction ; Dark field
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary Electron microscopical observations of the size and shape of bone mineral crystallites have not been in complete agreement with X-ray diffraction findings. The two prevalent viewpoints consider bone mineral crystals to be either rod, or plate like in habit. There appears to be agreement that the smallest dimension of the crystals is about 5 nm, but there is discrepancy in the reported c-axial lengths. The method of dark field imaging is used to obtain a quantitative measurement of the c-axial length distribution in rabbit, ox and human bone: mean c-axial lengths 32.6 nm, 36.2 nm and 32.4 nm, respectively, show no significant difference at the 5% level to the mean c-axial length measured by X-ray line broadening. Both bright and dark field images strongly suggest that bone mineral has a plate like form. Reasons for past discrepancies are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02010772
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  • 2
    Electronic Resource
    Electronic Resource
    Cellular calcium distribution in fetal bones studied with K-pyroantimonate (1978)
    Springer
    Calcified tissue international 26 (1978), S. 181-190 
    Details
    ISSN: 1432-0827
    Keywords: Cellular calcium ; Electron microscopy ; Osteoblasts ; Chondrocytes ; Mineralization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary The calcium distribution in cartilage and bone cells during beginning ossification of fetal mouse long bones was studied after fixation with 2% K-pyroantimonate in 1% osmium. In the developing periosteum, the future osteoblasts showed a sparse cation-antimonate precipitate over the cytoplasm. In young osteoblasts the precipitate was accumulated on the mitochondrial membranes and the plasmalemma. Both organelles were sharply outlined by precipitate in the mature osteoblasts at the onset of mineralization. X-Ray microprobe analysis of these organelles demonstrated the presence of both Sb and Ca. In the extracellular compartment, a collagen-associated precipitate with 50 to 60 nm periodicity appeared during osteoblastic differentiation. During the initial phase of matrix mineralization, a random gross precipitate appeared in the matrix and seemed to be accumulated by osmiophilic matrix vesicles while the collagen fibrils lost their precipitate. Subsequently, during the confluent phase of matrix mineralization, the precipitate rapidly disappeared from the cells, leaving them devoid of precipitate once they were surrounded by mineralized matrix. Similar changes were found in the chondrocytes of the growth plate, but cartilage collagen, unlike osteoid collagen, did not bind precipitate. The results indicate that both osteoblasts and calcifying cartilage cells bind calcium prior to matrix mineralization. Bone collagen has strong pyroantimonate binding capacity, but it is not directly involved with initial stages of matrix mineralization, which starts in close association with matrix vesicles.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02013254
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  • 3
    Electronic Resource
    Electronic Resource
    The fine structure of decalcified cartilage and bone: A comparison between decalcification procedures performed before and after embedding (1978)
    Springer
    Calcified tissue international 25 (1978), S. 179-190 
    Details
    ISSN: 1432-0827
    Keywords: Decalcification ; Electron microscopy ; Calcified matrices
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary The ultrastructure of calcifying cartilage and bone has been examined under the electron microscope after using three different methods of decalcification. The first was carried out before embedding (by soaking specimens in EDTA or formic acid), the second after embedding (by floating ultrathin sections on formic acid), and the third after embedding (by soaking embedded specimens in EDTA or formic acid), and with later re-embedding. The first procedure invariably induces drastic changes in the fine structure of the cells and calcified matrix, probably as a results of the extraction of organic material along with extraction of mineral. The second and third procedures make it possible to preserve ultrastructural details perfectly in both cells and calcified matrix. Of the two, the third procedure is preferable because of its greater simplicity. In areas that are still calcifying, these post-embedding decalcification techniques reveal the presence of crystal-associated, filamentous organic structures which are not recognizable in specimens decalcified before embedding. These structures, which could have a key role in inducing and regulating crystal formation and growth, are less evident in fully calcified areas (but not at their borders). This may partly be due to the loss of glycan components in the matrix during calcification. The most important determinant, however, seems to be the fact that during calcification the components of the matrix, including collagen fibrils, are involved in an aggregation process which reduces the amounts of free chemical groups available for reaction with the stain solution. Because post-embedding decalcification does not disturb this state of aggregation, the stainability of the matrix and the electron microscopic evidence of its components remain very low.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02010766
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  • 4
    Electronic Resource
    Electronic Resource
    The crystal sheaths from bivalve hinge ligaments (1978)
    Springer
    Calcified tissue international 25 (1978), S. 45-51 
    Details
    ISSN: 1432-0827
    Keywords: CaCO3 ; Amino acids ; Sheaths ; Ligament ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary The aragonite crystals in the molluscan bivalve hinge ligament are surrounded by an organic sheath which is distinct from the remainder of the ligament matrix. Methods have been developed to isolate these sheathed crystals from the ligaments ofSpisula solidissima andMercenaria mercenaria employing a papain digestion of the matrix protein. The sheathed crystals fromSpisula have a CaCO3/protein ratio of 11.1 and those fromMercenaria a ratio of 29.6. The sheathed crystals and the empty crystal sheaths have been examined by electron microscopy for structural integrity. The sheath proteins exhibit much smaller proportions of the amino acids glycine and methionine than the hinge ligaments. These are characteristic amino acids of high concentration in the hinge ligaments of both species. The concentrations of acidic and basic amino acids are increased about two fold in the sheaths over those of the ligaments. Otherwise there is little similarity in the amino acid composition of the sheaths in the two species. However, SDS electrophoresis shows the sheaths of both to contain a major protein component with a molecular weight of about 25,000. The sheath protein from theMercenaria ligament contains about 5% carbohydrate and that ofSpisula sheaths less than 1% carbohydrate.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02010750
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  • 5
    Electronic Resource
    Electronic Resource
    X-ray diffraction and electron microscope study of osteons during calcification (1978)
    Springer
    Calcified tissue international 25 (1978), S. 133-143 
    Details
    ISSN: 1432-0827
    Keywords: Osteon ; X-Ray diffraction ; Electron microscopy ; Calcification
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary To obtain information on the changes in the inorganic bone fraction during calcification, low- and wide-angle X-ray diffraction techniques and electron microscopy have been applied to single osteon samples. The samples were cylindrically shaped and their axes corresponded to the axes of the Haversian canals. The selection was made according to the degree of calcification and the orientation of collagen bundles and inorganic particles. Osteons at both the initial and final stages of calcification were chosen. Arrangements of fiber bundles and inorganic particles in successive lamellae characteristic of three types of osteon were selected, that is, longitudinally structured osteons, transversely structured osteons, and alternately structured osteons. The results indicate that in osteonic lamellar bone there are two types of inorganic particles: (1) granules arranged in linear or needle-shaped entities with maximum width 40–45 Å, which are regularly distributed at the level of the main band of the collagen fibrils where their maximum length reaches the length of the main band itself; that is, about 400 Å; and (2) very long crystallites, with a diameter of 40–45 Å, which grow with their crystallographicc-axis parallel to the collagen fibrils and cover much more than a major collagen period.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02010762
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  • 6
    Electronic Resource
    Electronic Resource
    A freeze-fracture study of tight junctions in the pars convoluta and pars recta of the renal proximal tubule (1978)
    Springer
    Cell & tissue research 186 (1978), S. 121-133 
    Details
    ISSN: 1432-0878
    Keywords: Proximal tubule ; Tight junctions ; Ultrastructure ; Electron microscopy ; Freeze fracturing
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The morphology of tight junctions of the renal proximal tubule was studied comparing the pars convoluta and pars recta of rat, golden hamster, rabbit, cat, dog and tupaia. Though some interspecies variations were observed, the convoluted portions of the proximal tubules revealed quite uniformly very leaky tight junctions with mainly 1–2 strands. Along the whole proximal tubule of the rabbit kidney including the pars recta only minor differences of the zonulae occludentes were found. By contrast, the tight junctions of the pars recta in other species were much more elaborate, especially in cat and tupaia, having up to 6 strands and an overall depth of more than 150 nm. The implications of these findings are discussed with special regard to the functional differences between the pars convoluta and pars recta of the proximal tubule.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00219659
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  • 7
    Electronic Resource
    Electronic Resource
    Localization of thyrotropin (TSH) in the dog pituitary gland (1978)
    Springer
    Cell & tissue research 186 (1978), S. 399-412 
    Details
    ISSN: 1432-0878
    Keywords: Pituitary gland ; Dog ; Pars distalis ; Thyrotropin (TSH) ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Using the immunoperoxidase technique and antisera to the specific beta (β) subunits of bovine and rat TSH1, selective immunocytochemical staining was localized in a specific cell population in the pars distalis of the dog pituitary gland. These TSH cells were found to be positive to aldehyde fuchsin, alcian blue, periodic acid-Schiff (PAS) and aniline blue. With the performic acidalcian blue (pH 0.2) -PAS-orange G procedure these cells stained blue-purple, demonstrating FSH/LH cells (blue or turquoise), ACTH/MSH cells (redpurple) and PRL cells (orange-red). The TSH cells were further differentiated from other functional cell types of the pars distalis on the basis of their typical cytological features, intraglandular distribution and by immunocytochemical double staining. In the pars distalis of adult male dogs the TSH cells were mostly shown to be smaller in size and less numerous than in bitches in the anestrous phase of the sexual cycle. Moreover, cytological alterations in the immunoreactive thyrotrophs in the pituitary of male and female dogs generally paralleled the spontaneous changes in thyroid function associated with thyroid atrophy and/or pituitary insufficiency, and thyroid hyperplasia or goiter. In conclusion, because of their specificity and high potency, the antisera to the β-subunits of bovine and rat TSH represent an effective tool for the selective immunocytochemical localization of TSH in the dog pituitary. This allows the study of the morphology and function of TSH cells under different physiological, pathological and experimental conditions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00224930
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  • 8
    Electronic Resource
    Electronic Resource
    Ultrastructure of the theca interna of ovarian follicles in sheep (1978)
    Springer
    Cell & tissue research 187 (1978), S. 457-472 
    Details
    ISSN: 1432-0878
    Keywords: Ovarian follicles (sheep) ; Theca interna ; Oestrogen ; Androgen ; Capillaries ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The theca interna of non-atretic ovarian follicles from 2.0 mm in diameter up to the stage shortly following ovulation was studied by light and electron microscopy. In follicles 〈3.0mm in diameter, the theca interna consisted of about 8–12 layers of flattened cells, together with many capillaries and small bundles of collagen. Two main forms of cellular differentiation were seen. These were towards either fibroblast-like cells or presumed steroidogenic cells whose cytoplasm contained large amounts of predominantly smooth tubular endoplasmic reticulum, to which some ribosomes were attached. The majority of cells were of relatively undifferentiated or intermediate structure. In larger follicles up to the early stages of oestrus the theca interna cells became larger and less flattened, and cells rich in tubular endoplasmic reticulum became proportionately more numerous. By 18 h after the onset of oestrus the theca interna was oedematous, and many cells possessed pseudopodia. Many cells also contained numerous lipid droplets, but there were no signs of thecal cell degeneration or death. Shortly after ovulation the basal lamina of the membrana granulosa was incomplete, and it became more difficult to distinguish between theca and granulosa layers. Structural heterogeneity, with two major cell types and cells of intermediate structure, was present at all stages. It was concluded that: (1) the theca interna of 2.0–2.9 mm follicles contained many cells whose structure was compatible with a steroidogenic capacity; (2) changes in the differentiated thecal cells up to the early stages of oestrus were quantitative rather than qualitative, and suggestive of an increased steroidogenic capacity; (3) the accumulation of lipid in many cells of the theca interna by 18 h after the onset of oestrus probably reflected a reduction in steroidogenic activity; and (4) there was no evidence of any structural specialization to facilitate the transport of steroids from the theca interna to the membrana granulosa.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00229610
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  • 9
    Electronic Resource
    Electronic Resource
    Local degenerative changes in the drum muscles of piranhas (Serrasalminae, Characidae) (1978)
    Springer
    Cell & tissue research 188 (1978), S. 75-82 
    Details
    ISSN: 1432-0878
    Keywords: Drum muscles ; Piranha ; Degenerative changes ; Fine structure ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In the posterior drum muscle of the piranha structural alterations are frequently observed. Electron microscopic studies revealed a disordering of the myofibrils in these regions, accompanied by conspicuous changes in the mitochondria. As the degree of disorder increases, the mitochondrial cristae initially swell to form vesicles. Eventually the mitochondria become filled with myelin-like lamellar structures, which in many cases coalesce to form a dense outer wall. A striking concentration of lysosomes into localized masses was also characteristic of these regions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00220515
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  • 10
    Electronic Resource
    Electronic Resource
    Immunocytochemical identification of an LHRH-producing system originating in the preoptic nucleus of the duck (1978)
    Springer
    Cell & tissue research 188 (1978), S. 99-106 
    Details
    ISSN: 1432-0878
    Keywords: LHRH-neurosecretion ; Avian hypothalamus ; Vasotocin neurosecretion ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A fluorescent technique applying specific LHRH and vasotocin antisera was used for the immunocytochemical localization of the respective neurosecretory systems in the hypothalamus of gonadectomized, testosteronetreated and/or serotonin injected male domestic ducks. An immunoreactive (IR) LHRH-producing system, with perikarya located in the preoptic nucleus, could be traced through the ventral hypothalamus down to the external layer of the rostral and caudal ME, in close vicinity to the hypophysial portal system. An IR-vasotocin system originating in the paraventricular and supraoptic nuclei ran through the ventral hypothalamus, but terminated in (i) the external layer of the rostral ME, and (ii) in the posterior lobe of the hypophysis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00220517
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  • 11
    Electronic Resource
    Electronic Resource
    Immunocytochemical study of the hypothalamo-neurohypophysial system (1978)
    Springer
    Cell & tissue research 188 (1978), S. 119-132 
    Details
    ISSN: 1432-0878
    Keywords: Neurophysin ; Paraventricular nucleus ; Supraoptic nucleus ; Sheep ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary An antiserum cross-reactive against ovine neurophysins-I-II and -III has been used in conjunction with the immunoperoxidase histochemical procedure to localize the cells of the sheep paraventricular (PVN) and supraoptic nuclei (SON). In order to describe the topographical distribution of the SON and PVN a study was made on the serial sections cut (a) transversely from rostral to caudal positions and (b) sagittally from lateral to medial positions of the hypothalamus. The cells of the SON, when examined in the transverse aspect, extended approximately 1900 μ caudally and when examined in the sagittal plane were contained within a lateral-medial distance of 4830 μ. In each case the SON cells lay adjacent to the optic chiasm. As sections were cut transversely, the cells of the PVN first appeared in a rostral position defined as 0 μ and close to the ventral lining of the third ventricle. This general ventral and ventro-lateral distribution of cells maintained up to a caudal distance of approximately 840 μ. From positions 1260–2310 μ there was a dramatic dorsal shift of the PVN cells which by this time had also extended laterally. The total rostral-caudal distance occupied by the PVN cells was 3150 μ. That the lateral-medial distance occupied by the PVN was small (1050 μ) was determined on examining the magnocellular nuclei in sagittal section.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00220519
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  • 12
    Electronic Resource
    Electronic Resource
    Studies on the ultrastructure of the integument of the rotifer Habrotrocha rosa Donner (Aschelminthes) (1978)
    Springer
    Cell & tissue research 189 (1978), S. 167-177 
    Details
    ISSN: 1432-0878
    Keywords: Hypodermis ; Rotifer ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The integument of the rotifer Habrotrocha rosa Donner is provided with pores and formed by an extrasyncytial cuticle and a syncytial hypodermis. The hypodermis peripherally contains 3 layers of dense cytoplasm and borders the cuticle by an asymmetric cell membrane. The wall of the pores is stiffened proximally like an annulus. The pores lead into cytoplasmic invaginations which are surrounded by vesicles. Close to and also beneath the condensed cytoplasmic layers microbodies are found, which are interpreted as microperoxisomes. Subhypodermal layers of muscles are connected with the cytoplasm of the hypodermis by desmosome-like structures.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00223127
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  • 13
    Electronic Resource
    Electronic Resource
    Catecholaminergic innervation of muscles in the hindgut of crustaceans (1978)
    Springer
    Cell & tissue research 189 (1978), S. 257-266 
    Details
    ISSN: 1432-0878
    Keywords: Neuromuscular junction ; Hindgut ; Crustacea ; Catecholamine ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The crustacean species Pacifastacus leniusculus and Gammarus pulex were investigated by electron microscopy in a search for possible neuromuscular junctions in the hindgut, which has a rich supply of catecholaminergic fibres. True neuromuscular synapses were found in both species between nerve terminals containing dense-core vesicles (80–110 nm in diam.) and muscle fibres. We suggest that the dense-core vesicle terminals contain a catecholamine, and this is supported by ultrahistochemical tests for monoamines. Two types of junctions are found: one in which the nerve terminal is embedded in the muscle cell (both species) and one in which protrusions from the muscle cell meet nerve terminals (Pacifastacus). Gammarus pulex, which has only circular muscles in the hindgut, has only catecholaminergic innervation, whereas Pacifastacus leniusculus has circular and longitudinal muscles both with at least two types of innervation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00209275
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  • 14
    Electronic Resource
    Electronic Resource
    Influence of maternal adrenalectomy on the ultrastructure of the adrenal gland in neonatal rats (1978)
    Springer
    Cell & tissue research 189 (1978), S. 277-286 
    Details
    ISSN: 1432-0878
    Keywords: Maternal adrenalectomy ; Rat ; Influence on the adrenals of newborn animals ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Maternal adrenalectomy at 7 or 14 days of gestation produced increased cell necrosis within zona reticularis cells on the day of birth and at 24 or 48 h after birth. Small remnants or large portions of adrenocortical cells were present within macrophages. In otherwise normal adrenocortical cells, lipid droplets were incorporated within some mitochondria. Autophagocytosis of single mitochondria was observed within adrenocortical cells. Undoubtedly ultrastructural changes represent stimulation of adrenocortical cells in neonatal rats in response to maternal adrenalectomy.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00209277
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  • 15
    Electronic Resource
    Electronic Resource
    Neuroendocrine cells within colorectal tumours induced by dimethylhydrazine (1986)
    Springer
    Cell & tissue research 246 (1986), S. 205-210 
    Details
    ISSN: 1432-0878
    Keywords: 1,2-Dimethylhydrazine ; Peptide YY ; Glucagon ; 5-Hydroxytryptamine ; Immunocytochemistry ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Colorectal adenocarcinomas were induced in male Wistar rats, by weekly subcutaneous administration of 1,2-dimethylhydrazine, classified according to the degree of differentiation and submitted to immunocytochemistry for the peptides cholecystokinin (CCK), gastrin, gastric inhibitory polypeptide (GIP), glucagon, neurotensin, pancreatic polypeptide (PP), peptide YY (PYY), somatostatin and vasoactive intestinal polypeptide (VIP) and the biogenic monoamine 5-hydroxytryptamine. Well- or moderately well-differentiated adenocarcinomas comprised 46% of the tumour population, only 4% were poorly-differentiated adenocarcinomas, and the remaining 50% possessed a mixture of these two morphologies. Glucagon, PYY and 5-hydroxytryptamine immunoreactive cells were frequently observed within well- or moderately well-differentiated tumours and within such regions of tumours possessing a mixed morphological pattern. The tumours contained no cells immunoreactive for any of the peptides not normally located within the colorectum, nor did they contain cells immunoreactive for somatostatin and VIP, although known positive controls did stain. Poorly-differentiated tumours and portions of tumours of mixed type, were consistently negative. 5-hydroxytryptamine was the most frequently located of the three antigens, being detected in 87% of the moderately well-differentiated tumours and 32% of the tumours with mixed morphologies. 11% of moderately well-differentiated tumours possessed 5-hydroxytryptamine positive cells in such profusion that they contributed significantly to the tumour mass. The distribution of glucagon-and PYY-immunoreactive cells was similar, although they occurred with a lower frequency, presumably corresponding to their lower numbers within the normal colorectal mucosa. Additionally, these two peptide immunoreactivities were colocalized in the majority of cells, although some cells contained only one antigen. The immense numbers of cells immunoreactive for peptides and monoamine in a significant proportion of colorectal adenocarcinomas suggests that they have arisen from multipotential endodermal stem cells within the tumours and are not part of the normal epithelial population being engulfed as the tumour grows.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00219019
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  • 16
    Electronic Resource
    Electronic Resource
    Structure of occluding junctions in ileal epithelial cells of suckling rats (1978)
    Springer
    Cell & tissue research 191 (1978), S. 449-462 
    Details
    ISSN: 1432-0878
    Keywords: Ileum (Rat) ; Occluding junctions ; Freeze-fracture ; Ultrastructure ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Two kinds of occluding junctions are found between ileal epithelial cells of suckling rats: apical zonulae occludentes (ZO) and fasciae occludentes (FO) which are associated with the lateral plasma membranes of many epithelial cells. In unfixed preparations, glycerol treatment induces the further proliferation of extensive fasciae occludentes. Both kinds of junction have identical structural elements when visualized in freeze fracture replicas, although the arrangement of these elements differs. Zonulae occludentes consist of networks of branching and anastomosing linear ridges or rows of ∼10 nm particles with 20–30 nm spaces between the rows which form narrow belt-like structures around the apical region of adjacent cells. Fasciae occludentes, on the other hand, consist of similar linear ridges or rows of particles but the junction strands are often discontinuous, open ended and only occasionally intersect with each other. Several different fracture planes through the plasma membrane in the region of the occluding junctions have been observed and these provide further evidence that two components, one from each membrane, fused at the level of the extracellular space, form the junction sealing element. Furthermore, we present evidence which indicates a staggered rather than an in-register arrangement of these two components.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00219808
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  • 17
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    Electronic Resource
    The ultrastructure of the accessory sex organs of the male rat (1978)
    Springer
    Cell & tissue research 192 (1978), S. 39-51 
    Details
    ISSN: 1432-0878
    Keywords: Prostate ; Rat-Anti-Androgens ; Nuclear alterations ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The fine structure of the nuclei of epithelial cells of the dorsal lobe of the rat prostate were studied after administration of three different antiandrogenic compounds. The nucleolus appears to undergo a progressive disorganisation with partial fragmentation and dispersion of its normal components. Different types of intranuclear inclusions were found. The various alterations observed were often encountered within the same section. This may indicate that the nuclear alterations occur in the same compartment of the cell, and represent a dysfunction of integrated biochemical events occurring within this compartment. The findings support a view that the stimulatory secretory effect of androgens is mediated via a ‘secretory center’, located within the nucleolusassociated chromatin. Within this ‘secretory center’, the initial steps of the secretory process, the binding of the DHT receptor complex to DNA is assumed to occur.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00231021
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  • 18
    Electronic Resource
    Electronic Resource
    Stereological model system for free cells and base-line data for human peripheral blood-derived small t-lymphocytes (1978)
    Springer
    Cell & tissue research 192 (1978), S. 121-142 
    Details
    ISSN: 1432-0878
    Keywords: T-lymphocytes ; Stereological model system ; Free cells ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Menschliche, in Nylonwolle gereinigte T-Lymphozyten aus dem peripheren Blut dienten als repräsentatives Untersuchungsobjekt zur Schaffung eines neuen stereologischen Modellsystems für freie, sphärische Zellen. Dieses System erlaubt, die Zelle und die darin enthaltenen Strukturkomponenten auf ultrastruktureller Ebene quantitativ zu charakterisieren.
    Notes: Summary T-lymphocytes derived from human peripheral blood and passed through a nylon-wool column, were employed to develop and test a new Stereological model system for free spherical cells, allowing a quantitative characterization of the cell and its components at the ultrastructural level. Electron micrographs were recorded in a hierarchical manner at three different levels of magnification and subjected to point counting procedures. The resulting parameters were expressed in relation to various reference compartments, both absolute and relative. Results indicated that the average volume of a small, non-activated T-lymphocyte was 103.8 μm3, the nuclear volume 47.5 μm3 and the cytoplasmic volume 55.9 μm3. On the average, the cytoplasm contained 30 mitochondria, 0.7 μm3 RER-cisternae, 0.2 μm3 cisternae and vesicles of the Golgi apparatus and about 231,000 free ribosomes (most of them single). The ratio of eu- to heterochromatin volume was 0.5. The design and application of the Stereological model system are discussed with regard to dynamic studies of a variety of free cells, such as macrophages, neutrophilic granulocytes and various lymphocytes.
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    URL: http://dx.doi.org/10.1007/BF00231028
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    Immunocytochemistry of magnocellular neurons of supraoptic and paraventricular nuclei of normal and Brattleboro rats with vasopressin anti-idiotype antibody (1986)
    Springer
    Cell & tissue research 246 (1986), S. 509-513 
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    ISSN: 1432-0878
    Keywords: Anti-idiotype antibody ; Vasopressin ; Immunocytochemistry ; Receptors ; Brattleboro rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A vasopressin anti-idiotype antibody was generated by immunization with purified IgG of a primary vasopressin antiserum. The anti-idiotype antibody immunostained neurons in the supraoptic and paraventricular nuclei of the hypothalamus of normal and Brattleboro rats. The distribution of immunostained perikarya in these hypothalamic nuclei together with the staining of fibers in median eminence and neural lobe was similar to that observed in normal rats with anti-vasopressin and suggests strongly that vasopressinergic neurons are being stained. Absorption studies with vasopressin and a vasopressin-binding receptor protein further indicate that a receptor associated with vasopressinergic neurons is recognized by the anti-idiotype antibody.
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    URL: http://dx.doi.org/10.1007/BF00215190
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    Immunocytochemical localization of a rhodopsin-like protein in the lipochondria in photosensitive neurons of Aplysia californica (1986)
    Springer
    Cell & tissue research 244 (1986), S. 115-120 
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    ISSN: 1432-0878
    Keywords: Neurons ; Lipochondria ; Rhodopsin ; Immunocytochemistry ; Aplysia californica
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Polyclonal antibodies directed against squid opsin were used in immunocytochemical and immunoblot experiments to identify a rhodopsin-like protein in photosensitive neurons of Aplysia. Aldehyde-fixed abdominal and cerebral ganglia were embedded in paraffin for peroxidase anti-peroxidase analysis or used whole for immunofluorescence studies. Ganglia were embedded in Lowicryl K4M for electron-microscope immunocytochemistry. In both the cerebral and abdominal ganglia, light-microscope immunocytochemical results showed reaction product deposited around the neuronal cell periphery corresponding in position to the lipochondria. In the abdominal ganglion, the giant cell R2, located in the right rostral quarter, and neurons in the right caudal quarter were consistently labeled with anti-opsin. Electron-microscopic studies demonstrated ferritin-labeling of the lipochondria in R2 and other immunoreactive neurons. Immunoblot analysis of R2 and cerebral neuron extracts was used to identify two prominent immunoreactive protein bands at 85000 and 67500 molecular weight.
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    URL: http://dx.doi.org/10.1007/BF00218388
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    GABA neurones in retinas of different species and their postnatal development in situ and in culture in the rabbit retina (1986)
    Springer
    Cell & tissue research 243 (1986), S. 117-123 
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    ISSN: 1432-0878
    Keywords: GABA ; Immunocytochemistry ; Neurones ; Retina ; Different species ; Rabbit
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The localisation of GABA immunoreactive neurones in retinas of a variety of animals was examined. Immunoreactivity was associated with specific populations of amacrine neurones in all species examined, viz. rat, rabbit, goldfish, frog, pigeon and guinea-pig. All species, with the exception of the frog, possessed immunoreactive perikarya in their retinal ganglion cell layers. These perikarya are probably displaced amacrine cells because GABA immunoreactivity was absent from the optic nerves and destruction of the rat optic nerve did not result in degeneration of these cells. GABA immunoreactivity was also associated with the outer plexiform layers of all the retinas studied; these processes are derived from GABA-positive horizontal cells in rat, rabbit, frog, pigeon and goldfish retinas, from bipolar-like cells in the frog, and probably from interplexiform cells in the guinea-pig retina. The development of GABA-positive neurones in the rabbit retina was also analysed. Immunoreactivity was clearly associated with subpopulations of amacrine and horizontal cells on the second postnatal day. The immunoreactivity at this stage is strong, and fairly well developed processes are apparent. The intensity of the immunoreactivity increases with development in the case of the amacrine cells. The immunoreactive neurones appear fully developed at about the 8th postnatal day, although the immunoreactivity in the inner plexiform layer becomes more dispersed as development proceeds. The immunoreactive horizontal cells become less apparent as development proceeds, but they can still be seen in the adult retina. The GABA immunoreactive cells in rabbit retinas can be maintained in culture. Cultures of retinal cells derived from 2-day-old animals can be maintained for up to 20 days and show the presence of GABA-positive cells at all stages. In one-day-old cultures the GABA immunoreactive cells lacked processes but within three days had clearly defined processes. After maintenance for 10 days a meshwork of GABA-positive fibres could also be seen in the cultures.
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    URL: http://dx.doi.org/10.1007/BF00221859
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    Lipokeratinocytes of the epidermis of a cetacean (Phocena phocena) (1986)
    Springer
    Cell & tissue research 244 (1986), S. 385-394 
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    ISSN: 1432-0878
    Keywords: Cetaceans ; Lamellar bodies ; Epidermal lipids ; Permeability barrier ; Electron microscopy ; Phocena phocena
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Biochemical and ultrastructural analysis of epidermis from the porpoise, Phocena phocena, revealed certain similarities and differences between cetaceans and terrestrial mammals. The predominant cell of cetacean epidermis, not found in normal terrestrial mammals, is a lipoker-atinocyte, which elaborates not only keratin filaments, but also two types of lipid organelles: first, lamellar bodies, morphologically identical to those of terrestrial mammals, are elaborated in great abundance in all suprabasal epidermal layers, forming intercellular lipid bilayers in the stratum corneum interstices: and second, non-membrane-bounded droplets appear and persist in all epidermal layers. Although the porpoise lipokeratinocyte morpologically resembles the sebokeratocyte of avians in certain respects, nonmembrane-bounded lipid droplets are not released into the intercorneocyte space as they are in avian stratum corneum. Whereas phospholipid/neutral lipid gradients are similar in porpoise and terrestrial mammals, PAS-positive glycoconjugates, specifically glycosphingolipids, are retained in porpoise stratum corneum, but lost from these layers in terrestrials. The novel, non-polar acylglucosyl-ceramides, which also are lost during cornification in terrestrial mammals, are retained in porpoise stratum corneum. The lipid components of porpoise lipokeratinocytes appear to subserve not only barrier function in a hypertonic milieu, but also underlie the unique buoyancy, streamlining, insulatory, and caloric properties exhibited as adaptations to the cetacean habitat.
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    URL: http://dx.doi.org/10.1007/BF00219214
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    Collagen remodeling during decidualization in the mouse (1986)
    Springer
    Cell & tissue research 244 (1986), S. 443-448 
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    ISSN: 1432-0878
    Keywords: Collagen ; Uterus ; Decidua ; Mouse ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary An ultrastructural study of the features and distribution of collagen fibrils was performed in the endometrium of virgin and pregnant (2nd to 11th day) mice. Collagen-containing structures were observed in the cytoplasm of fibroblasts on the 2nd day of pregnancy. Treatment of tissues with lanthanum nitrate established that these structures were intracytoplasmic. Their association with lysosome-like bodies suggested the occurrence of intracellular digestion of collagen, probably connected with remodeling of the endometrial stroma prior to decidualization. On the 4th day of pregnancy, very few collagen fibrils were present in the intercellular space. From the 6th day of pregnancy onwards, “thick” collagen fibrils were observed between decidual cells. The diameter of these fibrils measured up to 300 nm whereas the fibrils present in the endometrium of virgin mice measured 40–68 nm.
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    URL: http://dx.doi.org/10.1007/BF00219220
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    Morphological variation of immunoreactive cells positive to cholecystokinin 33 (10–20) and gastrin 34 (1–15) in human duodenum (1986)
    Springer
    Cell & tissue research 244 (1986), S. 519-525 
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    ISSN: 1432-0878
    Keywords: Duodenum ; Cholecystokinin ; Gastrin ; Immunocytochemistry ; Ultrastructure ; Man
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Human duodenal endocrine cells reactive with antibodies to cholecystokinin (CCK) 33 (10–20) and/or gastrin 34 (1–15) were studied by a combination of immunohistochemical and electron-microscopic methods. By immunohistochemistry, three types of endocrine cells were distinguished in human duodenal mucosa, i.e., those only positive for only CCK, those positive for both CCK and gastrin and those only positive for only gastrin. Ultrastructurally, the first cell type is characterized by many secretory granules with an eccentric dense core (mean diameter; 271+-74 nm). The second cell type, which was less frequent than the other two, has ultrastructural features that resemble type-I cells. The last cell type was composed of two types of cells containing small secretory granules identical to those of IG cells (mean diameter; 171+-31 nm) or large secretory granules indistinguishable from those of I cells (mean diameter; 286+-50 nm).
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    URL: http://dx.doi.org/10.1007/BF00212529
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    The dopaminergic innervation of the goldfish pituitary (1986)
    Springer
    Cell & tissue research 244 (1986), S. 577-582 
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    ISSN: 1432-0878
    Keywords: Dopamine ; Pituitary gland ; Neuroendocrine regulation ; Immunocytochemistry ; Carassius auratus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The dopaminergic innervation of the goldfish pituitary gland was studied by immunocytochemistry at the electron-microscope level using highly specific antibodies against dopamine coupled to bovine serum albumin with glutaraldehyde. A satisfactory preservation of the tissue was achieved after immersion in 5% glutaraldehyde in phosphate buffer containing sodium metabisulfite to prevent oxidation of the endogenous dopamine. The immunocyto-chemical procedure was performed on Vibratome sections using the preembedding method. Immunoreactivity was restricted to part of the neurosecretory type-B fibers (diameter of the secretory vesicles lower than 100 nm) in which it was found to occupy the whole cytoplasm. Labeled fibers were observed within the neurohypophysis in the different parts of the gland and in the adenohypophyseal tissue where immunoreactive profiles were detected in close apposition to the different cell types. These data are in agreement with previous results obtained by means of radioautography and further support a role for dopamine in the neuroendocrine regulation of pituitary functions in teleosts.
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    URL: http://dx.doi.org/10.1007/BF00212536
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    Glutamic acid decarboxylase (GAD)-like immunoreactivity in the pedal ganglion of Mytilus galloprovincialis (1986)
    Springer
    Cell & tissue research 244 (1986), S. 591-593 
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    ISSN: 1432-0878
    Keywords: Immunocytochemistry ; GAD ; Pedal ganglion ; Invertebrate nervous system ; Mytilus galloprovincialis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A substance immunologically related to vertebrate glutamic acid decarboxylase (GAD) has been visualized in the pedal ganglion of Mytilus with the pre-embedding peroxidase-antiperoxidase method, by use of an antiserum raised in sheep against rat brain GAD. The results show that GAD-like immunoreactivity is present both in neuronal perikarya and in nerve fibers. Positive neurons are located mainly among the fibers of the ganglion neuropil at the commissural level, and more rarely close to unreactive cortical cell bodies. Immunoreactive nerve fibers are observed throughout the neuropil and also in cerebropedal and pedal nerves.
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    URL: http://dx.doi.org/10.1007/BF00212538
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    Culture of intramural cardiac ganglia of the newborn guinea-pig (1986)
    Springer
    Cell & tissue research 244 (1986), S. 605-612 
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    ISSN: 1432-0878
    Keywords: Heart innervation ; Tissue culture ; Autonomic ganglia ; Non-neuronal cells ; Cell interrelationships ; Electron microscopy ; Guinea-pig
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The present study describes the ultrastructure of non-neuronal cells and their interrelationships with intracardiac neurones present in cultures dissociated atria and interatrial septum from newborn guinea-pig. When compared with the in situ preparation, most of these features in culture were similar to those observed in situ, but some differences were also apparent. Both mature and immature Schwann cells were observed in culture, and as in situ, the latter were closely associated with intracardiac neurones, whilst the former were more widely separated. The ultrastructure of satellite cells was more variable in culture than in situ: three general types were distinguished on the basis of their 10-nm filament content. This variation could be due to conditions of culture. Interstitial cells were present in culture and closely resembled those described in situ, although there was less space between cultured interstitial cells and their associated cells. Many fibroblasts, some myoblasts and a few mast cells were also found in the culture preparations.
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    Culture of intramural cardiac ganglia of the newborn guinea-pig (1986)
    Springer
    Cell & tissue research 244 (1986), S. 595-604 
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    ISSN: 1432-0878
    Keywords: Heart innervation ; Tissue culture ; Autonomic ganglia ; Neurones ; Small granule-containing cells ; Electron microscopy ; Guinea-pig
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The ultrastructure of cultured intrinsic neurones and SIF (small intensely fluorescent) cells dissociated from the atria and interatrial septum of newborn guinea-pig heart has been studied for the first time and compared with these cells in situ. Mononucleate and binucleate neuronal somata and their processes were observed in the culture preparation; their ultrastructure was similar to that of neurones in intracardiac ganglia observed in situ. The number of neurites associated with neuronal cell bodies increased after the first week in culture. A subpopulation of intracardiac neurones showed abnormalities in culture, comparable to the changes previously described in neurones of the monkey heart after unilateral vagotomy in situ. Small granule-containing cells were observed in culture, corresponding to those described in the heart in situ. One type of large process in the culture preparation containing densely packed mitochondria has not been seen in situ, suggesting that changes in cell ultrastructure due to the conditions of culture cannot be discounted. However, the ultrastructure of the cultured cells was, for the most part, consistent with that of the same cell type in situ, indicating that the culture preparation may be a useful model for investigation of the roles and interactions of intramural neurones in the heart, which are inaccessible for such studies in situ.
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    Redistribution of cell surface transferrin receptors prior to their concentration in coated pits as revealed by immunoferritin labels (1986)
    Springer
    Cell & tissue research 244 (1986), S. 613-619 
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    ISSN: 1432-0878
    Keywords: Cell-surface transferrin receptor ; Redistribution of cell-surface receptors ; Receptor-mediated endocytosis ; K562 cells ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Immunocytochemistry has been used to study distribution of cell surface transferrin receptors in erythroid, leukemic (K562) cells. The cells were fixed and labelled with monoclonal (OKT-9) anti-transferrin receptor antibodies; the antibody-labelled receptors were then detected by either immunofluoresceinor immunoferritin-antimouse-antibody conjugates. Typically, the immunoferritin labels were distributed diffusely at the non-coated regions of the cell surface as well as concentrated in the clathrincoated pits. To examine further this pattern of distribution, cells were labelled at 0° C and then warmed to 37° C for zero to 30 min prior to fixation. The majority of the immunoferritin labels were initially dispersed in small groups at the non-coated regions of the cell surface (mean = 6 immunoferritin labels/cluster), but larger groups were common subsequent to incubation at 37° C (mean = 13 immunoferritin labels/cluster). However, the size of immunoferritin labels in the coated pits was unchanged (mean = 12 immunoferritin labels/pit). Immunoferritin labels were typical in coated and uncoated vesicles l min after warming to 37° C, but common in endosomes, multivesicular bodies and lysosomes by 30 min. It appears that single cell-surface receptors form large aggregates prior to their concentration in coated pits. Coated vesicles, uncoated vesicles, and endosomal vacuoles may together form the non-lysosomal compartment where the internalized receptors might be dissociated from the ligands (antibodies).
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    URL: http://dx.doi.org/10.1007/BF00212541
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    Morphological evidence for the existence of a more complex cytoskeleton in Amoeba proteus (1986)
    Springer
    Cell & tissue research 246 (1986), S. 163-168 
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    ISSN: 1432-0878
    Keywords: Cytoskeletal organization ; Filaments ; Triton extraction ; Replica technique ; Electron microscopy ; Amoeba proteus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Various stabilization and extraction procedures were tested to demonstrate the ultrastructural organization of the cytoskeleton in normal, locomoting Amoeba proteus. Most reliable results were obtained after careful fixation in glutaraldehyde/lysine followed by prolonged extraction in a polyethylene glycol/Triton X-100 solution. Before dehydration in a graded series of ethanol and critical-point drying, the amoebae were split by the sandwich-technique, i.e., by mechanical cleavage of cells mounted between two poly-L-lysine-coated glass slides. Platinum-carbon replicas as well as thin sections prepared from such cell fragments revealed a cytoskeleton composed of at least four different types of filaments: (1) 5–7-nm filaments organized as a more or less ordered cortical network at the internal face of the plasma membrane and probably representing F-actin; (2) 10–12-nm filaments running separately or slightly aggregated through the cytoplasm and probably representing intermediate filaments; (3) 24–26-nm filaments forming a loose network and probably representing microtubules; and (4) 2–4-nm filaments as connecting elements between the other cytoskeleton constituents. Whereas microfilaments are responsible for protoplasmic streaming and other motile phenomena, the function of intermediate filaments and cytoplasmic microtubules in amoebae is still obscure.
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    An autoradiographic study of satellite cell differentiation into regenerating myotubes following transplantation of muscles in young rats (1978)
    Springer
    Cell & tissue research 186 (1978), S. 535-540 
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    ISSN: 1432-0878
    Keywords: Skeletal muscle ; Satellite cells ; Regeneration ; Electron microscopy ; Autoradiography
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Satellite cells were traced autoradiographically during the regeneration of skeletal muscle in young Sprague-Dawley rats. Approximately 31% of the satellite cells in uninjured muscles appeared labelled after three injections of tritiated thymidine; none of the myonuclei were labelled in the same muscles. Four to six days after transplanting the radioactive muscles to non-radioactive littermates, regenerating myotube nuclei in the host appeared labelled. Thus, this study confirms that satellite cells in young rats can differentiate into multinucleated myotubes following muscle injury.
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    Endocrine cells in the gut of the ascidian Styela clava (1978)
    Springer
    Cell & tissue research 187 (1978), S. 159-165 
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    ISSN: 1432-0878
    Keywords: Endocrine cells ; Ascidian (Styela clava) ; Gut epithelium ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Ultrastructural studies have shown the presence of two types of granulated endocrine cell in the gut of Styela clava. Type I, which occurs in the stomach and intestine contains small irregular granules, each with a distinct halo. Type II, found only in the oesophagus contains larger rounded granules, often with little or no halo. The characteristics of these two cell types are compared with those of endocrine cells found in the digestive tracts of other protochordates and discussed with special reference to the evolution of gastrointestinal endocrine cells in vertebrates.
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    The junctional connections between the cells of the urinary bladder in the rat (1978)
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    Cell & tissue research 187 (1978), S. 439-448 
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    ISSN: 1432-0878
    Keywords: Urinary bladder epithelium (Rat) ; Gap junctions ; Tight junctions ; Electron microscopy ; Freeze-fracturing
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The junctional connections between the cells of the urinary bladder epithelium in rat have been studied by freeze-fracturing. Tight junctions and desmosomes are known as structural features of the epithelium. In addition, gap junctions (nexus) have been found to connect the epithelial cells in an irregular distribution pattern. The junction size ranges from few assembled particles up to plaques with irregular forms. This may indicate that the gap junctions are mobile structures. The functional significance of the junctions in comparison with electrophysiological data is discussed.
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    The passage of spermatozoa through the vitelline membrane in the domestic fowl, Gallus gallus (1978)
    Springer
    Cell & tissue research 188 (1978), S. 497-508 
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    ISSN: 1432-0878
    Keywords: Fertilization ; Domestic fowl ; Acrosome reaction ; Vitelline membrane ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The developing outer layer of the vitelline membrane of the ovum in the posterior part of the infundibulum of the domestic fowl contains many spermatozoa in nearly parallel orientation with its inner layer. When the acrosomal region of a spermatozoon approaches or contacts the inner layer, promptly undergoes the acrosome reaction. The outer acrosomal membrane and overlying plasma membrane fuse together and the apical region of the acrosome opens, so that the acrosomal contents are released. Meanwhile the spermatozoon remains a time in contact with the surface of the inner layer, and the network of the inner layer just under the tip of the sperm head begins to be dissolved. This dissolution extends downward forming a tunnel, approximately 9 μm in diameter. The spermatozoon then passes through the inner layer obliquely via the central region of the tunnel and arrives at the perivitelline space.
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    A study of the fine structure of the pit organ of the common Japanese sea eel Conger myriaster (1978)
    Springer
    Cell & tissue research 189 (1978), S. 375-388 
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    ISSN: 1432-0878
    Keywords: Lateral line organ ; Pit organ ; Sea eel (Conger myriaster) ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The fine structure of the pit organ of the sea eel has been studied by means of electron microscopy. The sensory epithelium of the pit organ consists of sensory cells and supporting cells. The apical surface of the sensory cell is studded with sensory hairs consisting of a kinocilium and stereocilia. The sensory cells are divided into two groups. In one, the kinocilium points dorsally and in the other the kinocilium points ventrally. The total number of sensory cells in one pit organ is about 100, and the ratio of cells with opposite polarity is about 1∶1. On the basis of these structural features, the pit organ is considered to be a mechano-receptor sensitive to the movement of liquid in a dorso-ventral direction. It may also serve as an ion receptor, sensitive to environmental ion concentration. Efferent nerve terminals make rare synaptic contacts on the afferent nerve fiber.
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    On the excretory system of the rotifer Habrotrocha rosa donner (1978)
    Springer
    Cell & tissue research 189 (1978), S. 515-523 
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    ISSN: 1432-0878
    Keywords: Protonephridium ; Rotifer ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The excretory system of Habrotrocha rosa consists of two protonephridia. From each of them seven tubules lead to a terminal organ, also called cyrtocyte. Its weir basket contains structures which resemble ciliary rootlets. The lumen of the excretory system is bordered by a syncytial layer of cytoplasm. Cells are only found in the upper region of the trunk, where the channel is twisted several times.
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    Studies on the ultrastructure of the rotifer Habrotrocha rosa Donner (Aschelminthes) (1978)
    Springer
    Cell & tissue research 189 (1978), S. 525-535 
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    ISSN: 1432-0878
    Keywords: Alimentary system ; Rotifer ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The alimentary system of Habrotrocha rosa is composed of the alimentary channel, five digestive glands and another gland which is in close relation to the intestinal syncytium. After the present investigation, the alimentary channel can be divided into sections which clearly differ from each other, these are: oral cavity, pharynx, oesophagus, mastax, stomach-hose, syncytium of intestine, and terminal intestine.
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    Immunocytochemical localization of S-100 protein in stellate cells (folliculo-stellate cells) of the adenohypophysis in the monkeys Macaca irus and Cercopithecus aethiops (1986)
    Springer
    Cell & tissue research 246 (1986), S. 237-242 
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    ISSN: 1432-0878
    Keywords: S-100 protein (human, bovine) ; Folliculo stellate cells ; Adenohypophysis ; Immunocytochemistry ; Monkeys (Macaca irus, Cercopithecus aethiops)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary With the use of an antibody against bovine S-100 protein, it was possible to reveal a characteristic cell type in the pars distalis and the pars tuberalis of the monkey Macaca irus. In the adenohypophysis of Cercopithecus aethiops, labeled cells were present in the pars distalis, pars tuberalis, and pars intermedia. These cells, so-called folliculo-stellate cells, were found in all pituitaries studied. Surprisingly, an antibody against human S-100 protein did not label the stellate cells of the adenohypophysis. However, in Macaca irus, this antibody gave a strong positive reaction with various other cell types (interstitial cells of the pineal gland, Müller cells of the retina, autonomic ganglionic cells, glial cells of the central nervous system, Schwann cells, Bergmann glia of the cerebellum, fat cells, reticular cells of lymphoid organs). By use of double immunoenzymatic labeling, it was evident that stellate cells are spatially related either to somatotropes, prolactin cells, “corticotropes”, or to glycoprotein-containing cells. Thus, a specific relationship to a particular endocrine-cell type could not be observed.
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    5-Hydroxytryptamine immunoreactivity in the epidermal sacciform gland cells of the clingfish Lepadogaster candollei Risso (1986)
    Springer
    Cell & tissue research 246 (1986), S. 679-682 
    Details
    ISSN: 1432-0878
    Keywords: Fish skin ; Sacciform glandular cells ; Immunocytochemistry ; 5-Hydroxytryptamine ; Lepadogaster candollei
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Serotonin has been demonstrated in the epidermal sacciform glandular cells of the clingfish Lepadogaster candollei by use of immunocytochemistry. Serotonin immunoreactivity is found both in the peripheral cytoplasm of the glandular cells and their luminal secretion. The presence of serotonin in the sacciform glandular cells parallels that located by both biochemical and immunocytochemical procedures in the cutaneous glands of many amphibian species.
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    URL: http://dx.doi.org/10.1007/BF00215211
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    Immunocytochemical study of the development of vasotocin/mesotocin in the hypothalamo-hypophysial system of the chick embryo (1986)
    Springer
    Cell & tissue research 243 (1986), S. 15-31 
    Details
    ISSN: 1432-0878
    Keywords: Embryonic hypothalamo-hypophysial system ; Vasotocin neurons ; Mesotocin neurons ; Chick embryo hypothalamus ; Immunocytochemistry ; Chicken
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The hypothalamo-hypophysial system of the chick embryo has been studied with a monoclonal antibody which cross-reacts with arginine vasotocin and mesotocin, using thick (100 μm) sections in conjunction with a peroxidase-conjugated rabbit anti-mouse antibody. Although weakly stained perikarya occur occasionally in the tuberal region on embryonic days 6 and 7, the most consistent immunostaining of perikarya is found in the periventricular region of the caudal midhypothalamus at the level of the optic chiasm after embryonic day 8 1/2. Synthesis of peptides, therefore, takes place while the cells are close to their site of origin. Between embryonic days 9 and 10, beaded axons run along the anterior median eminence closely apposed to the adenohypophysis, thereby forming the anlage of the zona externa. The axons of the hypothalamo-neurohypophysial tract surround the neural lobe between embryonic days 11 and 12. The caudal to rostral wave of neuronal maturation that occurs during development appears to be due to a progressive differentiation of the periventricular zone, as well as the migration of perikarya. The early periventricular perikarya at embryonic day 8 1/2 send processes rostrally in a wing-shaped formation that extends both dorso- and ventrolaterally. From embryonic days 10 to 12, perikarya can be observed in the wing-like extensions, apparently migrating to rostral levels. The dorsolateral pathway gives rise at its midportion to the lateral cell group, whereas those perikarya migrating more laterally form the anlage of the external supraoptic nucleus. The ventrolateral wing-shaped extension of perikarya appears to be directed toward the ventral group and those lateral perikarya continuous with it. The location of mature neuronal cell groups is well established by embryonic day 17.
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    Immunocytochemical localization of ornithine decarboxylase in cultured murine cells (1986)
    Springer
    Cell & tissue research 243 (1986), S. 33-40 
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    ISSN: 1432-0878
    Keywords: Ornithine decarboxylase ; Macrophage ; Immunocytochemistry ; Murine cell culture ; Antibody specificity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Antiserum elicited to ornithine decarboxylase (ODC) purified from murine RAW 264 macrophage-like cells has been employed to localize ODC in cultured murine cells. The antiserum immunoprecipitated 100% of the ODC activity from the cultured cells. The specificity of the antiserum was demonstrated by the immunoprecipitation from 35S-methionine metabolically-labeled cell extracts of a single protein which migrated upon SDS-gel electrophoresis coincident with authentic ODC. Indirect immunofluorescence experiments were performed on paraformaldehyde-fixed RAW 264 cells and JB6 epidermal cells using the rabbit anti-ODC antiserum and FITC-conjugated goat anti-rabbit IgG. Little immunofluorescence was apparent in non-stimulated cells. Intense immunofluorescence was detectable in stimulated cells at times of peak cellular ODC activity. Antigenically-reactive ODC was localized diffusely in the cytoplasm and was absent in the nuclei of RAW 264 cells, whereas in the JB6 cells the immunodetectable enzyme protein was localized in a punctate pattern in both the cytoplasm and nucleoplasm and was absent in the nucleolus. The appearance and disappearance of immunoreactive ODC in both cell types after stimulation was consistent with the alterations in ODC activity.
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    Structure and development of the surface coat of erythrocytic merozoites of Plasmodium knowlesi (1986)
    Springer
    Cell & tissue research 245 (1986), S. 281-290 
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    ISSN: 1432-0878
    Keywords: Electron microscopy ; Malaria parasites ; Merozoites ; Surface coat ; Maturation ; Plasmodium knowlesi
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The surface of extracellular merozoites of P. knowlesi is covered with a coat 15–20 nm thick, made up of clusters of filaments standing erect on the plasma membrane. Filaments have stems 2 nm thick, the peripheral ends of which are complex, branching or ending in long trailing threads. Coat filaments occur on the surface of the parasite in regular rows at an early schizont stage, and persist until well after merozoite release. They are sensitive to trypsin and papain, and bind ethanolic phosphotungstate, indicating a proteinaceous nature. They are also removed by exposure to phosphate-buffered saline. Filaments bear negative charges, binding cationised ferritin throughout the depth of the coat and staining with ruthenium red. They cover the whole merozoite surface and mediate intercellular adhesion at distances of 15–150 nm, membrane to membrane. It is suggested that these filaments correspond to a major merozoite surface protein, and are important in the initial capture of red cells.
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    Immunoreactivity of the hermaphroditic gonad of the snail Helix aspersa Müller towards antibodies raised to fragments of pre-pro-opio-melanocortin (1986)
    Springer
    Cell & tissue research 245 (1986), S. 337-341 
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    ISSN: 1432-0878
    Keywords: Immunocytochemistry ; Hermaphrodite gonad ; Pre-pro-opio-melanocortin ; FMRF-amide-like materials ; Snail, Helix aspersa Müller
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Male and female germinal cells of the active hermaphroditic gonad of a snail (Helix aspersa Müller) reveal a positive immunoreactivity to some antibodies raised against biologically active peptides related to pre-pro-opiomelanocortin of vertebrates. All but the oldest cells of the different spermatogenetic and oogenetic stages are methionine-enkephalin-immunopositive, whereas only the young oocytes are α-MSH- and 17–39 ACTH-positive. Sometimes some male cells show an 1–24 ACTH positivity. Structures other than germinal cells also react with some antibodies: for example, the nurse cells are β-MSH-immunoreactive, the nerve fibers surrounding each acinus and the hermaphrodite duct are both 17–39 ACTH and FMRF amide positive.
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    Differential immunocytochemical staining for glial fibrillary acidic (GFA) protein, S-100 protein and glutamine synthetase in the rat subcommissural organ, nonspecialized ventricular ependyma and adjacent neuropil (1986)
    Springer
    Cell & tissue research 245 (1986), S. 343-351 
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    ISSN: 1432-0878
    Keywords: Subcommissural organ ; Ependyma ; Astrocytes ; Immunocytochemistry ; Glial fibrillary acidic (GFA) protein ; S-100 protein ; Glutamine synthetase ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Antibodies raised against glial fibrillary acidic protein (GFA), S-100 protein (S100) and glutamine synthetase (GS) are currently used as glial markers. The distribution of GFA, S100 and GS in the ependyma of the rat subcommissural organ (SCO), as well as in the adjacent nonspecialized ventricular ependyma and neuropil of the periaqueductal grey matter, was studied by use of the immunocytochemical peroxidase-antiperoxidase technique. In the neuropil, GFA, S100 and GS were found in glial elements, i.e., in fibrous (GFA, S100) and protoplasmic astrocytes (S100, GS). The presence of S100 in the majority of the ventricular ependymal cells and tanycytes, and the presence of GFA in a limited number of ventricular ependymal cells and tanycytes confirm the glial nature of these cells. The absence of S100, GFA and GS from the ependymocytes of the SCO, which are considered to be modified ependymal cells, suggests either a non-astrocytic lineage of these cells or an extreme specialization of the SCO-cells as glycoprotein-synthesizing and secreting elements, a process that may have led to the disappearance of the glial markers.
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    Immunocytochemical identification of structures containing putative red pigment-concentrating hormone in two species of decapod crustaceans (1986)
    Springer
    Cell & tissue research 245 (1986), S. 377-386 
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    ISSN: 1432-0878
    Keywords: Red pigment-concentrating hormone ; Immunocytochemistry ; Neurosecretion ; Crustaceans ; Carcinus maenas ; Orconectes limosus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary By use of an antiserum raised against the Nterminal sequence pGlu-Leu-Asn-Phe..., common to red pigment-concentrating hormone (RPCH) of Pandalus borealis and three structurally similar insect neuropeptides, putative RPCH-immunopositive structures were revealed in the eyestalks of Carcinus maenas and Orconectes limosus and in the brain and thoracic ganglion (TG) of C. maenas. In the eyestalks, complete neurosecretory pathways were demonstrated, consisting of perikarya, axons and terminals in the neurohemal organ, the sinus gland (SG). In C. maenas approximately 20 small RPCH cells are present as a distinct group adjacent to the medulla terminalis ganglionic X-organ (MTGXO, XO). They are morphologically different from the larger XO perikarya, which contain the crustacean hyperglycemic hormone (CHH). The occurrence of both neuropeptides in distinct neurosecretory pathways was ascertained by immunologic double staining (PAP/gold) or by analysis of consecutive sections. In addition, a group of two to four larger RPCH cells is located in the proximal part of the MT. In O. limosus, RPCH cells are found in the XO. Cells corresponding to the proximal MT cells of C. maenas were not found. In both species, a few more weakly staining immunopositive perikarya were observed in clusters of cell somata of the optic ganglia. It is uncertain whether these are connected to the SG. In the brain of C. maenas, several smaller and three larger perikarya were consistently observed in the dorsal lateral cell somata adjacent to the olfactory lobes. In the optic nerve, two axons that project into the eyestalk were stained. Some axons were also observed in the ventral median neuropil of the brain. In the TG, RPCH cells were found in small numbers in median positions, i.e., in clusters of somata between the ganglia of the appendages. HPLC analysis of the red pigment-concentrating activity from the SG of C. maenas revealed that the retention time of the neuropeptide is similar but not identical to that of Pandalus borealis RPCH.
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    Nephridial innervation in the leech Hirudo medicinalis L. (1986)
    Springer
    Cell & tissue research 245 (1986), S. 397-404 
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    ISSN: 1432-0878
    Keywords: Leech ; Nephridium ; Innervation ; Electron microscopy ; Cobalt filling ; Hirudo medicinalis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The main organs for salt and water homeostasis in the medicinal leech, the nephridia, were found to be densely innervated by a single branch of the corresponding median anterior segmental nerve. The projections of two different neurons into the nephridia are described: 1. Dendritic projections of the previously identified, afferent ‘nephridial nerve cell’, a possible salt receptor, lie between the urine forming cells and the blood vessels supplying the nephridium without making any contact. 2. Projections of an unidentified neuron which contains dense-core vesicles (85 nm) as well as smaller clear vesicles (45 nm) contact the primary urine forming canaliculus cells. The neurosecretory role of these neurons is considered.
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    Choline acetyltransferase-like immunoreactivity in the brain of Drosophila melanogaster (1986)
    Springer
    Cell & tissue research 246 (1986), S. 57-62 
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    ISSN: 1432-0878
    Keywords: Insect brain ; Neurotransmitters ; Immunocytochemistry ; Drosophila melanogaster
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Using a monoclonal antibody selective for the acetylcholine (ACh)-synthesizing enzyme choline acetyltransferase (ChAT) of Drosophila melanogaster we find ChAT-like immunoreactivity in specific synaptic regions throughout the brain of Drosophila melanogaster apart from the lobes and the peduncle of the mushroom body and most of the first visual neuropile (lamina). Several anatomically well-defined central brain structures exhibit particularly strong binding. Characteristic differential staining patterns are observed for each of the four neuromeres of the optic lobes. Cell bodies appear not to bind this antibody. The prominent features of the distribution of ChAT-like immunoreactivity are paralleled by the distribution of acetylcholine hydrolyzing enzymatic activity as revealed by histochemical staining for acetylcholine esterase (AChE). These results are discussed in comparison with published data on enzyme distribution, choline uptake and ACh receptor binding in the nervous system of Drosophila melanogaster.
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    Mosquito trypsin: immunocytochemical localization in the midgut of blood-fed Aedes aegypti (L.) (1986)
    Springer
    Cell & tissue research 245 (1986), S. 19-27 
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    ISSN: 1432-0878
    Keywords: Trypsin ; Immunocytochemistry ; Midgut ; Exocytosis ; Aedes aegypti
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A polyclonal antibody was raised against trypsin purified from the midgut of blood-fed Aedes aegypti. Using this antibody and our modification of the peroxidase-antiperoxidase immunocytochemical reaction, strong activity was found in the lumen of the midgut at the light-microscopical level. The activity was localized mainly in the posterior part of the distensible, abdominal midgut, along the periphery of the blood bolus and within the peritrophic membrane. Immunoreactivity appeared 8 h after the blood meal and was most prominent around 24 h, coinciding with our previous spectrophotometric determinations of trypsin. At the electron-microscopical level, secretory granules, immunocytochemically labelled with anti-trypsin antibody and protein A-colloidal gold, were first detected about 12 h after the blood meal. At 18 h, the secretory pathway could be followed immunocytochemically from the formation of granules in the Golgi complex until their release by exocytosis in the midgut lumen. By 24 h, there was a reduction in secretory granules, and large lysosomes appeared. The process of secretion described for this mosquito is comparable to similar events in vertebrate secretory systems and the presence of an intracellular trypsinogen is suggested.
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    Somatotrophs and lactotrophs in the anterior pituitary of fetal and neonatal rats (1986)
    Springer
    Cell & tissue research 245 (1986), S. 457-460 
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    ISSN: 1432-0878
    Keywords: Anterior pituitary ; Somatotroph ; Lactotroph ; Ultrastructure ; Immunocytochemistry ; Fetal and neonatal rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The ultrastructure of immunoreactive somatotrophs and lactotrophs in pituitaries of fetal rats at 19, 20 and 21 days of gestation and on the day of birth was studied. Somatotrophs, first detectable at 19 days of gestation, undergo only minor modifications before reaching the structure described for adults. In particular there is an increase in the endoplasmic reticulum and Golgi apparatus. Lactotrophs, first identifiable in newborn rats, are very different in ultrastructure from adult cells, because the secretory granules are generally small, but variable in shape and size, and the Golgi complex is prominent.
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    Nerve-purkinje fiber relationship in the moderator band of bovine and caprine heart (1978)
    Springer
    Cell & tissue research 188 (1978), S. 11-18 
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    ISSN: 1432-0878
    Keywords: Purkinje fibers (Ox, goat) ; Axons ; Nerve endings ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The moderator band in the heart of the ox and goat contains bundles of Purkinje fibers and nerve fibers separated by connective tissue. The axons are mostly unmyelinated and embedded in the cytoplasm of Schwann cells. Small bundles of axons run close to the Purkinje fibers. The axons dilate into varicosities 0.5 to 1.6 μ in diameter (mean 0.95 μ), containing three types of vesicles: 1) agranular vesicles with a diameter of 400–500 Å, 2) large dense-cored vesicles with a diameter of 800–1200 Å, 3) small dense-cored vesicles with a diameter of 500 Å. Most varicosities contain agranular vesicles together with a few large dense-cored vesicles. The gap between the varicosities and the nearest Purkinje fiber is unusually wide and normally varies between 0.3 μ and 0.8 μ. No intimate nerve-Purkinje fiber contacts, with a cleft of 200 Å, were observed.
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    Immunocytological evidence for oxytocin neurons in the human fetal hypothalamus (1978)
    Springer
    Cell & tissue research 188 (1978), S. 259-264 
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    ISSN: 1432-0878
    Keywords: Hypothalamus ; Human fetus ; Oxytocin ; Neurophysin ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The use of antibodies against oxytocin or neurophysin enabled the detection by immunocytochemistry of oxytocin-neurophysin neurons in the hypothalamus in the human fetus. The perikarya of these neurons are located in the paraventricular and supraoptic nuclei. Immunoreactive neurons occur in the median eminence. The neurophysin immunoreactive neurons were more numerous than the oxytocin immunoreactive neurons. The specificity of the immunocytological reaction was controlled. The first oxytocin-neurophysin neurons are seen as early as the 14th week of gestation.
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    On T-tubule openings at the sarcolemma of white fast-twitch muscle fibres in fish and frog (1978)
    Springer
    Cell & tissue research 188 (1978), S. 265-272 
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    ISSN: 1432-0878
    Keywords: Muscles ; T-system ; Fish ; Frog ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In “white” muscle fibres of a teleost fish T-tubule openings may occur regularly at all Z-disc levels between adjacent peripheral myofibrils, the T-tubule openings thus occurring at a density of ca. 0.9 μm-2. In frog “white” fibres, T-tubule openings are infrequently seen in material fixed like the fish material. In material prepared according to the albumin method of Gray (1975, 1976 a, b) which renders the muscle fibres swollen, straight tubules or sometimes chains of vesicles instead are seen opening at the sarcolemmal surface. Such tubules occur at a higher density than expected from experiments with local activation of contraction. Lability and dynamics within the T-system normally and during fixation are discussed.
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    Parenchymal fine structure of the subfornical organ in the Japanese quail, Coturnix coturnix japonica (1978)
    Springer
    Cell & tissue research 191 (1978), S. 405-419 
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    ISSN: 1432-0878
    Keywords: Subfornical organ ; Japanese quail, Coturnix coturnix japonica ; Monoaminergic innervation ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The parenchyma of the subfornical organ (SFO) of the Japanese quail was studied by light and electron microscopy. The SFO consists of ependymal, intermediate, and basal (perimeningeal) layers. In the intermediate layer, neurons, glial cells, and their processes are found. Axons containing dense core granules approximately 80 nm in diameter are numerous, some of which make synaptic contact with the neuronal perikarya or dendrites. Synaptic vesicles in some axons contain a dense dot in the interior after treatment with 5-hydroxydopamine. The activity of the SFO, which is probably concerned with elicitation of drinking by angiotensin II, may be regulated at least partly by afferent monoaminergic axons. Capillaries with a non-fenestrated endothelium are occasionally found in the parenchyma. The basal layer is occupied by glial processes abutting on the digitating layer of perivascular connective tissue of meningeal vessels. The endothelium of these vessels is occasionally fenestrated. Trypan blue injected systemically accumulated in the SFO, but not in the deeper areas of the brain. The absence of a blood-brain barrier is suggested in the SFO.
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    Sequential differentiation of intestinal endocrine cells in the fetal mouse (1978)
    Springer
    Cell & tissue research 192 (1978), S. 267-276 
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    ISSN: 1432-0878
    Keywords: Endocrine glands ; Mouse small intestine ; Mouse embryo ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The duodenum and ileum of 15 to 18-day old Swiss ICR mouse embryos were studied with the electron microscope to follow the differentiation of endocrine cells. Endocrine cells were classified on purely morphological characteristics. EC cells appeared at 16 days of gestation in both segments. At the same stage G cells were seen in the duodenum and K cells in the ileum. ECL, and S cells were identified in the duodenum at 17 days. Finally, D cells were seen at 18 days of gestation in the duodenum and ileum. With the electron microscope, endocrine cells were not identified in the small intestine of the mouse before the formation of villi.
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    Localization and function of an FMRFamide-like substance in the aorta of Helix aspersa (1986)
    Springer
    Cell & tissue research 246 (1986), S. 303-307 
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    ISSN: 1432-0878
    Keywords: FMRFamide ; Aorta ; Bioassay ; Immunocytochemistry ; Helix aspersa
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary With an antiserum (aFM) against the molluscan “cardio-active” FMRFamide (Phe-Met-Arg-Phe-NH2) numerous immunoreactive axons were found in the outer, longitudinal, muscle layer of the anterior aorta of Helix aspersa. Immunoreactive axons were rare in the inner, circular, muscle layer. At the ultrastructural level four types of axons could be distinguished. The granules containing the immunoreactive substance (mean diameter ca. 100 nm) are present in type-2 axons. The effect of synthetic FMRF-amide was tested in vitro on preparations of ring- and tubule-shaped pieces of the anterior aorta. Physiological doses (3 × 10-7 M) provoked contractions of the circular muscle fibres, but had no effect on the longitudinal muscle cells. Apparently in vivo the FMRF-like substance diffuses from the richly innervated longitudinal muscle layer to the circular muscle layer, where it exerts its effect. This conclusion is sustained by the observation that the contents of the aFM-immunoreactive granules in type-2 axons are released by exocytosis in a “non-synaptic” fashion.
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    The coxo-trochanteral muscle receptor organ of locusts (1986)
    Springer
    Cell & tissue research 243 (1986), S. 517-524 
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    ISSN: 1432-0878
    Keywords: Muscle receptor organ ; Electron microscopy ; Tubular body ; Mechanosensory transduction ; Locust, Locusta migratoria migratorioides (R.&F.)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The coxo-trochanteral muscle receptor organ of the hind leg of the locust Locusta migratoria migratorioides (R.&F.) has been investigated by use of scanning and transmission electron microscopy with special emphasis on its distal attachment site. The overall morphology of the receptor muscle, the sensory neuron and its dendrites was found to share many common features with other arthropod sense organs of that type with two important differences: (1) the connective tissue segment (= intercalated tendon) is extremely short compared to that of other muscle receptor organs; (2) the naked dendritic terminals of the non-ciliated, multipolar sensory neuron of the organ contain clusters of microtubules, interconnected by an amorphous matrix, that resemble the tubular bodies of ciliated, epithelial receptor cells.
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    Light and electron-microscopic immunocytochemistry and lectin histochemistry of the subcommissural organ: Evidence for processing of the secretory material (1986)
    Springer
    Cell & tissue research 243 (1986), S. 545-559 
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    ISSN: 1432-0878
    Keywords: Subcommissural organ ; Glycoproteins ; Secretory process ; Immunocytochemistry ; Lectin histochemistry ; Rat ; Vertebrates
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The subcommissural organ (SCO) of the rat was investigated by use of histochemical and immunocytochemical methods at the light and electron-microscopic levels. Consecutive thin methacrylate sections were stained with the pseudoisocyanin (Psi), immunoperoxidase (IMC; employing an antiserum against Reissner's fiber, AFRU), periodic acid-Schiff (PAS) and periodic acid-silver methenamine (SM) techniques, and reacted with six types of lectins. Psi, SM, concanavalin A (Con A) and IMC were also used for double and triple sequential staining of the same section. Increasing dilutions of AFRU (from 1∶1000 to 1∶200 000) were used for immunostaining of serial paraffin sections. In addition, ultrastructural localization of (i) Con A-binding sites and (ii) immunoreactive secretory material was performed. Some of these procedures were also applied to the ophidian and canine SCO. Con A-positive, Psi-positive and immunoreactive materials coexisted within the same cisternae of the rough endoplasmic reticulum. The Golgi apparatus lacked Con A-positive and immunoreactive substances. Apical secretory granules and secreted material lying on the surface of the SCO showed (i) the highest affinity for AFRU, but were (ii) Con A-negative, and (iii) wheat-germ agglutinin-, PAS and SM-positive. Reissner's fiber displayed a low affinity for AFRU. It is suggested that the SCO secretes N-linked glycoproteins, the carbohydrate and protein moeities of which undergo (i) a maturation process before being released, and (ii) some kind of modification(s) after their release into the ventricle. The perivascular secretory cells of the dog SCO might secrete a material different from that secreted by the ependymal cells.
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    URL: http://dx.doi.org/10.1007/BF00218061
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    The hypothalamo-hypophysial system of the frog Rana temporaria (1986)
    Springer
    Cell & tissue research 243 (1986), S. 649-654 
    Details
    ISSN: 1432-0878
    Keywords: Median eminence ; Peptidergic projections ; Aminergic projections ; Electron microscopy ; Autoradiography ; Frog (Rana temporaria)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The median eminence (ME) of the adult frog, Rana temporaria, was studied by means of electron microscopy including quantitative electron-microscopic autoradiography. In frogs captured in May and June numerous peptidergic neurosecretory fibres extending via the internal zone to the pars nervosa display large swellings containing few granules, mitochondria, neurotubules and cisternae of the smooth endoplasmic reticulum. In addition, few secretory globules up to 1.5 μm in diameter occur in these varicosities. In animals collected during the autumn period many of these neurosecretory swellings filled with neurosecretory granules and polymorphic inclusions resemble Herring bodies. Three types of granule-containing neurosecretory fibres were observed in the external zone (EZ) of the ME of adult R. temporaria. Peptidergic A1- and A2-type fibres are characterized by granules 150–220 nm and 100–160 nm in diameter, respectively. Monoaminergic fibres of type B with granules approximately 100 nm in diameter represent ∼ 50% of all neurosecretory elements in the EZ of the frog ME; ∼12% of the total number of granule-bearing axons in the EZ actively taking up radiolabelled 5-hydroxytryptophan are thought to be serotoninergic terminals. Neurosecretory terminals of all types and glial vascular endfeet establish direct contacts with the perivascular space of the primary portal capillaries. Some neurosecretory terminals are separated from the lumen of the third ventricle by a thin cytoplasmic lamella of tanycytes. The possible physiological significance of this structural pattern is discussed.
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    URL: http://dx.doi.org/10.1007/BF00218074
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    Correlative immuno-electron-microscopic and immunofluorescent localization of actin in sensory and supporting cells of the inner ear by use of a low-temperature embedding resin (1986)
    Springer
    Cell & tissue research 245 (1986), S. 229-235 
    Details
    ISSN: 1432-0878
    Keywords: Inner ear ; Cochlea ; Actin ; Immunocytochemistry ; Chinchilla
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The cochleas from chinchilla inner ears were processed in the cold through Lowicryl K4M, and cured by UV light. Thick (2 μm) sections were reacted with primary antibodies raised against actin, and anti-actin antibodies localized by FITC epifluorescence. On thin sections from the same blocks anti-actin antibodies were localized ultrastructurally with secondary antibodies coupled to colloidal gold. In the hair cells, actin was present in the stereocilia and cuticular plate, regions where thin filaments were observed by electron microscopy. Colloidal gold was uniformly distributed over these regions and over the stereocilia rootlets demonstrating that actin was present in this region although previously in permeabilized cells, the rootlet was not decorated with myosin subfragment S-1. Actin was present in the pillar and Deiters supporting cells at the reticular lamina and at the basilar membrane, where a meshwork of thin filaments was seen by electron microscopy. Colloidal gold particles were also localized over the thin processes of the pillar and Deiters cells, and over the region of the Deiters cell which envelops the base of the outer hair cell. In these regions actin co-localized with microtubules along the entire length of the supporting cells.
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    URL: http://dx.doi.org/10.1007/BF00213926
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    Immunocytochemical study of fibronectin in the sea lamprey, Petromyzon marinus, and the Atlantic hagfish, Myxine glutinosa (1986)
    Springer
    Cell & tissue research 244 (1986), S. 549-555 
    Details
    ISSN: 1432-0878
    Keywords: Fibronectin ; Lamprey ; Hagfish ; Immunocytochemistry ; Connective tissues
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Immunoreactive fibronectin-like material was localized within tissues of agnathans (hagfishes and lampreys) by an immunoperoxidase technique. Fibronectin was detected in basement membranes and in loose and dense connective tissues throughout the agnathan body. A fibronectin-like component was also identified in the plasma of both lampreys and hagfishes. The results indicate that fibronectin or a fibronectin-like material is a major component of agnathan connective tissues. Although there were some variations in the localization of fibronectin both between the lamprey and the hagfish and between agnathan and other vertebrate tissues, the generalized pattern of distribution of fibronectin in the agnathans supports the view that this protein, like that in higher vertebrates, plays a role in cellmatrix adhesion and tissue organization.
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    URL: http://dx.doi.org/10.1007/BF00212532
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    An ultrastructural study of embryonic chick retinal neurons in culture (1986)
    Springer
    Cell & tissue research 245 (1986), S. 563-577 
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    ISSN: 1432-0878
    Keywords: Chick retina ; Tissue culture ; Electron microscopy ; Development, ontogenetic ; Differentiation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The differentiation of cells and synapses in explants of 9-day-old chick embryo retina has been studied by light and electron microscopy over a period of 35 days in vitro, and samples of retina from the 9-day chick foetus were directly fixed and prepared for study. At the time of explantation the retinae were poorly differentiated and no lamination was apparent. From day 14 onwards, (i) outer and inner nuclear layers (ONL, INL) separated by a layer of neuropil corresponding to the outer plexiform layer (OPL) and (ii) a layer of scattered large ganglion cells separated from the INL by a zone of neuropil resembling the inner plexiform layer (IPL) were apparent, and (iii) a well-differentiated outer limiting membrane was established close to the surface of the explants. In the oldest cultures some development of photoreceptor outer segments occurred but a distinct optic nerve fibre layer did not form. Although cell identification presented problems even in the oldest cultures, the major retinal cell types described in vivo could be identified. Photoreceptor cells developed pedicles in the OPL which became filled with synaptic vesicles and synaptic ribbons and established ribbon synapses (including triads) with and were commonly invaginated by processes from horizontal and bipolar cells. Processes of bipolar cells in the IPL formed simple and dyad synapses. At least two types of presynaptic amacrine cells were also identified in the INL, one of which contained large numbers of dense-core vesicles. The ganglion cells, though sparse, were large and well differentiated. These findings show that all the major neuronal types of the retina are capable of developing and differentiating in vitro, lagging behind the time-table of development and differentiation in vivo by approximately 7 days, but resulting in a histotypically organised retina with synaptic neuropil showing many similarities to the corresponding neuropil in vivo.
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    URL: http://dx.doi.org/10.1007/BF00218558
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    Ultrastructure and central projections of extraocular photoreceptors in caddiesflies (Insecta: Trichoptera) (1986)
    Springer
    Cell & tissue research 245 (1986), S. 643-648 
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    ISSN: 1432-0878
    Keywords: Photoreceptors, extraocular ; Optic lobes ; Stemmata ; Immunocytochemistry ; Insecta (Trichoptera)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Retained larval eyes (stemmata) were studied in the imagines of three species of Trichoptera: Phrygania grandis, Agrypnia varia, and Trichostegia minor. At the light-microscopic level the stemmata of all three species appeared to represent different stages of reduction with respect to size, shape and number of lenses. However, in all three species electron-microscopic studies showed units with monolayered rhabdoms, each formed by four retinula cells. By use of immunocytochemistry the presence of S-antigen was demonstrated in the retinula cells and their axons. This method also revealed the central projections of the axons of the retinula cells, which were found (i) to terminate either in the lamina accessoria or (ii) to penetrate this area to join the fibers of the outer chiasma of the optic lobes and then terminate in the medulla accessoria. The lamina accessoria and the medulla accessoria are the assumed remnants of the larval optic lobes. It is suggested that the imaginal stemmata might still be functioning photoreceptors.
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    Localization of enkephalin immunoreactivity in diverse tissues and cells of the developing and adult rat (1986)
    Springer
    Cell & tissue research 246 (1986), S. 561-565 
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    ISSN: 1432-0878
    Keywords: Growth ; Enkephalin ; Endogenous opioids ; Immunocytochemistry ; Differentiation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The distribution of enkephalin, an endogenous opioid, in tissues and cells of the developing and adult rat was determined by immunocytochemistry with antibodies to met- and leu-enkephalin. Met- and leu-enkephalin were found in all developing cells investigated, with staining generally located throughout the cytoplasm; cell nuclei were not immunoreactive. In comparison to developing cells, immunoreactive analogues to met-enkephalin were usually difficult to detect in the adult. Some notable exceptions were reaction products in leukocytes in blood, lung, and cortex of thymus, fibroblasts in the skin, and seminiferous tubules. These results, in concert with earlier reports that opioid receptors are found largely in developing, but not adult, tissues, indicate that endogenous opioids are specifically involved in biological development, particularly cell proliferation and differentiation. Immunoreactivity in adult nonneural cells may be related to their development in some cases, but also could indicate other functions.
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    Localization of substance P-like immunoreactive fibers in the thoracic spinal cord of guinea pig (1986)
    Springer
    Cell & tissue research 246 (1986), S. 653-665 
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    ISSN: 1432-0878
    Keywords: Substance P ; Spinal cord ; Dorsal-horn fiber system ; Immunocytochemistry ; Guinea pig
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A dorsal-horn fiber system is revealed in the thoracic spinal cord of guinea pig by means of substance P immunocytochemistry. This system has repeated craniocaudal and/or caudo-cranial extensions and possesses five main components: (1) a superficial network, situated beneath the dorsolateral surface of the spinal cord. This network is connected with the dorsal root fibers and the accumulations of substance P-like immunoreactive (SP-LI) fibers in the Lissauer's tract; (2) an accumulation of SP-LI fibers in the Lissauer's tract at the border of the dorsal horn; (3) two collateral SP-LI fascicles (one lateral and one medial) emerging from the SP-LI fiber accumulation in the Lissauer's tract; (4) a transversal fascicle running through laminae III–V, and (5) an SP-LI network in the region of the lateral spinal cord nucleus. These components of the dorsal-horn fiber system show widespread connections with ipsi-and contralateral spinal cord areas, connecting them in cranio-caudal and/or caudo-cranial directions. The SP-LI dorsal-horn system has close relationship with groups of preganglionic sympathetic cells in the intermediate zone of the spinal cord, respective with the vegetative network of this zone. It is suggested that some fibers of the dorsal-horn system that originate from dorsal-root ganglia may represent primary sensory or visceral afferents. It is likely that the dorsal-horn fiber system and the vegetative network of the thoracic spinal cord may represent the morphological basis for the integration of (1) the central and peripheral vegetative nervous systems, and (2) the somatic and vegetative nervous system.
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    The myelination of the cerebellar cortex in the cat (1978)
    Springer
    Cell & tissue research 188 (1978), S. 509-520 
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    ISSN: 1432-0878
    Keywords: Cerebellar cortex (Cat) ; Postnatal development ; Myelination of efferent and afferent fiber systems ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The myelination of the cerebellar cortex of the cat was investigated in 61 cats aged from 3 hrs post partum to two and a half years. The first myelinated fibers appear at the time of birth in the central medullary ray. Before the onset of myelination, all fibers reach a critical diameter of about 1 μm. About the 14th day of life the number of oligodendrocytes in the prospective white matter increases markedly. Thereafter, the oligodendrocytes invade the inner granular layer. It therefore seems that the myelination of the cerebellar cortex proceeds from the central medullary ray towards the granular layer. At the 60th day of postnatal life, most of the afferent and efferent fiber systems are myelinated. These findings are discussed in relation to the development of function and the maturation of the electrical activity of the cerebellar circuit.
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    The interstitial cells in the renal medulla of rat, rabbit, and gerbil in different states of diuresis (1978)
    Springer
    Cell & tissue research 189 (1978), S. 1-18 
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    ISSN: 1432-0878
    Keywords: Kidney medulla ; Rat, rabbit, gerbil ; Interstitial cells ; Lipid droplets ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The inner zone of the renal medulla of rats, gerbils, and rabbits was investigated to determine whether or not there are any characteristic ultrastructural differences between the interstitial cells of these species. The effects on the interstitial cells of water deprivation and water loading were also investigated. In all three species, the Type 1 interstitial cells, the lipid containing cells, were abundant and their distribution and topographical relations as well as their general ultrastructure were similar. The previously reported significantly higher frequency in desert rats could not be confirmed. Although the lipid droplets of the interstitial cells were smaller in gerbils and rabbits when compared to rats, their fine structure was similar. Their electron dense outer zone was sometimes associated with a granular material and/or a lamellar material with a periodicity of about 40 Å resembling phospholipid “myelin figures”. Water-loaded rats showed a considerable increase in the number of lipid droplets when compared to dehydrated or untreated animals. In contrast, the interstitial cells of waterloaded gerbils and rabbits were depleted of lipid droplets.
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    Differential subcellular localization of ACTH and α-MSH in corticotropes of the rat anterior pituitary (1986)
    Springer
    Cell & tissue research 243 (1986), S. 229-238 
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    ISSN: 1432-0878
    Keywords: Anterior pituitary ; Corticotropes ; α-MSH ; Immunocytochemistry ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Specific antisera to α-melanotropin (α-MSH) and corticotropin (ACTH 1-39) were used to obtain immunocytochemical evidence for the differential localization of α-MSH and ACTH in the secretory granules of corticotropes of rat anterior pituitary. The specificity of the antisera was established by binding 131I-labeled α-MSH and ACTH 1-39 to their respective antisera. Double-labeling immunocytochemistry (for α-MSH, ferritin; for ACTH, colloidal gold) was performed. Some secretory granules were labeled with ferritin particles (α-MSH), whereas others contained gold particles (ACTH). Only a few granules showed both ACTH and α-MSH. In typical corticotropes (stellate in form with a small number of secretory granules aligned along the cell periphery) only some of the secretory granules that were labeled with anti-ACTH serum were also immunoreactive to anti-α-MSH. In atypical corticotropes (polygonal in shape and containing a large number of secretory granules) almost all of the immunoreactive ACTH secretory granules were also positive to anti-α-MSH serum. An intermediate type of corticotrope was observed containing a small number of secretory granules, almost all of which were labeled with anti-α-MSH. Thus, rat anterior pituitary corticotropes may be classified into three types according to the distribution and content of α-MSH. The light-microscopic immuncytochemistry provided similar results.
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    The organization of serotonin-immunoreactive neuronal systems in the brain of the crested newt, Triturus cristatus carnifex Laur. (1986)
    Springer
    Cell & tissue research 243 (1986), S. 239-247 
    Details
    ISSN: 1432-0878
    Keywords: Serotonin ; Indoleamines ; Aminergic neurons ; Immunocytochemistry ; Central nervous system ; Crested newt (Triturus cristatus carnifex)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The distribution of serotonin (5-HT) immunoreactive structures has been investigated in the brain of the crested newt by means of indirect immunofluorescence, and unlabeled antibody peroxidase-antiperoxidase-complex (PAP) or biotin-avidin-system (BAS) techniques. In the newt, the bulk of the serotoninergic system extends from the raphe region of the medulla oblongata, through the isthmus, toward the mesencephalic tegmentum, and is characterized by pyriform neurons mainly located in a subependymal position, close to the midline. Also in the caudal hypothalamus, in addition to some 5-HT-positive adenohypophysial cells, many immunoreactive CSF-contacting neurons are found lining the paraventricular organ and the nucleus infundibularis dorsalis. A rich serotoninergic innervation was observed in the preoptic area and in the habenular complex. Concerning the telencephalon, immunopositive nerve fibers are encountered in the dorsal pallium, primordium hippocampi, striatum and olfactory bulbs. The general organization of serotoninergic systems in the newt brain exhibit close similarities to that described in higher vertebrates.
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    Immunocytochemical evidence for the presence of “mammalian” neurohormonal peptides in neurones of the tapeworm Diphyllobothrium dendriticum (1986)
    Springer
    Cell & tissue research 243 (1986), S. 41-49 
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    ISSN: 1432-0878
    Keywords: Neuropeptides ; Serotonin ; Diphyllobothrium dendriticum ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In the nervous system of the obligatory endoparasite Diphyllobothrium dendriticum immunoreactivity (IR) to growth hormone-releasing factor (GRF), peptide histidine isoleucine (PHI), bovine pancreatic polypeptide (BPP), gastrin, gastrin-releasing peptide (GRP), oxytocin, FMRF-amide (FMRF) and serotonin (5HT) was demonstrated by immunocytochemical methods. A very strong GRF-IR was observed in the CNS and PNS of larvae and of the constantly growing adult worms. GRF-IR axon terminals occur beneath the basal lamina of the tegument along the inside of the bothridia, the holdfast organ of the worm. GRF-IR fibres surround the yolk producing vitelline glands and occur in the wall of the vagina. PHI-IR was observed in the CNS and PNS of larvae and adult worms. PHI-IR terminals occur beneath the basal lamina of the tegument along the strobila, the nutrient absorbing surface of the worm. PHI-IR fibres seem to innervate the testicular follicles. FMRF-IR fibres and perikarya occur close to the vitelline glands and the uterine pore and in the male copulatory organ. Numerous large 5HT-IR perikarya with long varicose fibres were observed in the nervous system of the worm. 5HT-IR perikarya occur close to the genital atrium. D. dendriticum is the phylogenetically lowest organism in which IR to PHI has been demonstrated.
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    Immunocytochemical differentiation between adipokinetic hormone (AKH)-like peptides in neurons and glandular cells in the corpus cardiacum of Locusta migratoria and Periplaneta americana with C-terminal and N-terminal specific antisera to AKH (1986)
    Springer
    Cell & tissue research 243 (1986), S. 9-14 
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    ISSN: 1432-0878
    Keywords: Adipokinetic hormone ; Corpus cardiacum ; Locusta migratoria ; Periplaneta americana ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary An immunocytochemical method was used to differentiate between immunoreactive substances in glandular cells in the corpora cardiaca (CC) and in certain cerebral neurons in 2 insect species, Locusta migratoria migratorioides and Periplaneta americana. The staining properties of antisera raised to different parts of the decapeptide adipokinetic hormone (AKH) were compared and their specificity was determined by preabsorption with AKH and related peptides. Antibodies raised to the N-terminal part of AKH (serum 433) and the central and C-terminal part (serum 241) were found to have different staining properties. In the CC of the locust both antisera show a strong immunoreactivity with glandular cells, we therefore suggest that at least one of the compounds revealed is AKH. Some of the glandular cells in the locust and large numbers of glandular cells in the CC of the cockroach are revealed by the N-terminal specific antiserum. On the other hand, neurons in the central nervous system are revealed only by the C-terminal specific antiserum. The possible identity of the various substances revealed by these two antisera is discussed.
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    Embryonic cholinesterase activity during morphogenesis of the mouse genital tract (1986)
    Springer
    Cell & tissue research 244 (1986), S. 153-164 
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    ISSN: 1432-0878
    Keywords: Cholinesterase ; Genital tract ; Mesenchyme ; Histochemistry ; Electron microscopy ; Mouse embryo
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In the genital tract of male and female mouse embryos cholinesterase activity is described that is independent from innervation. The enzyme activity is localized in the mesenchyme at the junction of Wolffian and Müllerian ducts with the urogenital sinus. During male development prostate buds and vesicular glands grow out into the cholinesterase-active mesenchyme. During female development the active mesenchyme participates in the downgrowth of the vaginal anlage. Ultrastructurally the cholinesterase activity is localized in the perinuclear cisterna and in smooth endoplasmic reticulum of the mesenchymal cells. The enzyme activity disappears with definitive differentiation of the tissue. The embryonic cholinesterase is a component of a primitive muscarinic system. Its relation to the morphogenetic action of testosterone and its possible general functions are discussed.
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    Comparative immunocytochemical study of release sites of insulin, glucagon and AKH-like products in Locusta migratoria, Periplaneta americana, and Carausius morosus (1986)
    Springer
    Cell & tissue research 245 (1986), S. 267-271 
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    ISSN: 1432-0878
    Keywords: Peripheral neurosecretory structures ; Immunocytochemistry ; Insulin ; Glucagon ; AKH ; Insects
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Insulin, glucagon and adipokinetic hormone antisera were applied to the corpora cardiaca, perisympathetic organs, neurohemal areas and peripheral neurosecretory cells of three insect species, the locust Locusta migratoria, the cockroach Periplaneta americana, and the stick insect Carausius morosus. The neurohemal part of the corpora cardiaca was shown to be immunoreactive to both insulin and glucagon antisera while the glandular cells reacted to adipokinetic hormone antisera. The perisympathetic organs seem to be devoid of these three substances, but certain peripheral neurohemal areas contained AKH and glucagon immunoreactive products. The latter were found to originate in the peripheral neurosecretory cells.
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    Immunocytochemical demonstration of 5-HT-like and FMRF-amide-like substances in whole mounts of Microstomum lineare (Turbellaria) (1986)
    Springer
    Cell & tissue research 246 (1986), S. 7-12 
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    ISSN: 1432-0878
    Keywords: 5-HT ; FMRF-amide ; Immunocytochemistry ; Nervous system ; Microstomum lineare (Turbellaria)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A whole mount immunofluorescence method is used for an investigation of immunoreactivity (IR) to anti-(a-) 5-HT and anti-(a-)FMRF-amide in the nervous system (NS) of Microstomum lineare (Turbellaria, Macrostomida, Platyhelminthes). New details of the organization of the NS are demonstrated, differences in 5-HT and FMRF amide IR are revealed, and new information on the development of the NS in zooids is obtained. In contrast to previous reports of a reduction (one pair of nerve cords without transverse processes) of the basic turbellarian plan, IR to both antisera reveals three pairs of longitudinal nerve cords, and features of the orthogonal organization, characterized by transverse commissures. The lateral pair of nerve cords is the most prominent. The following differences in the patterns of 5-HT and FMRF-amide IR are observed: 1. Perikarya positive for a-5-HT and a-FMRF-amide in the brain show different localizations. 2. Perikarya positive for a-5HT occur along the main lateral nerve cords, while the cords visualized by FMRF-amide IR look double-stranded and lack associated perikarya. 3. 5-HT IR is observed in a postpharyngeal commissure, which is absent in the a FMRF-amide-stained preparations. 4. In developing zooids 5-HT IR is first observed in the postpharyngeal commissure and later on in an increasing number of perikarya and in the neuropile. The first FMRF-amide IR in developing zooids appears in the cerebral commissure and in two perikarya in front of this commissure.
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    Immunocytochemical demonstration of growth hormone, prolactin and somatostatin-like immunoreactivities in the brain of larval, young adult and upstream migrant adult sea lamprey, Petromyzon marinus (1986)
    Springer
    Cell & tissue research 246 (1986), S. 23-31 
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    ISSN: 1432-0878
    Keywords: Growth hormone ; Prolactin ; Somatostatin ; Immunocytochemistry ; Lamprey
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Growth hormone, prolactin and somatostatinlike immunoreactivities were demonstrated in the brains of larval, young adult (parasitic) and upstream migrant adult sea lampreys, Petromyzon marinus, by means of immunoperoxidase techniques. Growth hormone (GH) and prolactin (PRL) were observed within separate perikarya in the nucleus praeopticus, within fibers in the commissura praeinfundibularis, and in nerve endings within the neurohypophysis of larval and adult-stage lampreys. Cell bodies demonstrating immunoreactive growth hormone were more numerous than those reactive for prolactin. Unlike in the upstream migrant adult lamprey, no GH or PRL was demonstrated in the adenohypophysis of larval or parasitic lamprey. Somatostatin (SRIF)-like immunoreactive neurons were demonstrated in the nucleus commissurae praeinfundibularis, anterior and posterior pars ventralis hypothalami, pars dorsalis thalami, and the tegmentum motorium rhombencephali of larval, parasitic and upstream migrant adult lampreys. Many of the SRIF containing neurons within the hypothalamus were cerebrospinal fluid (CSF)-contacting cells. SRIF fibers were found throughout most of the brain predominating within the nucleus praeopticus, pars ventralis hypothalami, and the nucleus interpeduncularis. No SRIF immunoreactivity was found within the neurophyophysis. The possible functions of these peptides within the brain of the lamprey are discussed.
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    URL: http://dx.doi.org/10.1007/BF00218994
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    The shape and distribution of astrocytes in the retina of the adult rabbit (1986)
    Springer
    Cell & tissue research 246 (1986), S. 91-102 
    Details
    ISSN: 1432-0878
    Keywords: Retina ; Astroglia ; Perivascular glia ; Glial fibrillary acidic protein (GFAP) ; Nerve fiber layer ; Immunocytochemistry ; Rabbit
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Astrocytes stained by antibodies to glial fibrillary acidic protein (GFAP) were examined in whole-mount preparations of retinae from adult rabbits and found to be restricted to the medullary rays. Astroglial cells exhibited a variety of shapes that varied between two extreme morphologies. One extreme was an astrocyte that possessed a few sturdy primary processes as well as finer processes and was strongly GFAP positive. The other extreme was an astroglial cell that displayed a star-shaped appearance; its perikarya gave rise to a few thin, radially oriented processes, which were rather weakly GFAP positive. The majority of astroglial processes were aligned with the ganglioncell axons, but some of their processes were in contact with capillaries. It has been proposed that astrocytes are specifically associated with ganglion-cell axons. Their restriction to the medullary rays in the retina of the rabbit suggests, however, that their physiological role is also concerned with the vascular system.
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    URL: http://dx.doi.org/10.1007/BF00219004
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    Immunocytochemical demonstration of calcitonin-containing c-cells in the thyroid glands of different mammals (1978)
    Springer
    Cell & tissue research 186 (1978), S. 551-558 
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    ISSN: 1432-0878
    Keywords: C-cells ; Thyroid gland ; Immunocytochemistry ; Calcitonin antibody ; Mammals
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In the thyroid glands of the horse, pig, deer, mole, and rat, C-cells could be demonstrated by means of the immunocytochemical PAP-technique using rabbit antisera against human calcitonin. Only in ruminants, the crossreaction between the intracellularly stored antigen and the antibodies used appeared to be incomplete.
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    URL: http://dx.doi.org/10.1007/BF00224943
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    Role of zinc and calcium in the formation and storage of insulin in the pancreatic β-cell (1978)
    Springer
    Cell & tissue research 188 (1978), S. 107-118 
    Details
    ISSN: 1432-0878
    Keywords: Pancreatic β-cells (mouse) ; Formation and storage of insulin ; Role of zinc and calcium ; Culture of islets ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary 1. The effects of culture of isolated mouse islets of Langerhans for up to 9 days in media which had been depleted of zinc electrochemically or with the chelating agent Tris-(2-aminoethyl) amine, or of calcium, have been compared. 2. An 83% reduction of extracellular zinc concentration did not adversely affect proinsulin biosynthesis, conversion of proinsulin to insulin, or the ability of cells to store newly formed insulin in granules. When incubation media were depleted of both zinc and calcium the β-cells produced abnormally large electron-lucent granules, consistent with the failure of insulin to crystallise within the granule sac. 3. Very similar results, with formation of large electron lucent granules, were obtained after culture of islets in the absence of calcium but in the presence of normal concentrations of zinc. 4. It is suggested that zinc may play a less critical role in the biosynthesis of proinsulin and its conversion to insulin, while calcium may have a more important function in insulin storage, than has sometimes previously been supposed.
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    URL: http://dx.doi.org/10.1007/BF00220518
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    Light and electron microscopic immunocytochemical localization of thyroglobulin in the thyroid gland of the anadromous sea lamprey, Petromyzon marinus L., during its upstream migration (1978)
    Springer
    Cell & tissue research 187 (1978), S. 473-478 
    Details
    ISSN: 1432-0878
    Keywords: Thyroid gland ; Immunocytochemistry ; Thyroglobulin ; Petromyzon marinus L
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Antibodies made against thyroglobulin (TG) were used in an immunocytochemical study for the light and electron microscopic localization of TG in the thyroid gland of the anadromous sea lamprey, Petromyzon marinus, during its upstream migration. TG was found in the follicular lumen and in some colloid droplets within the follicular cells. Except for an immunoreactive product observed in a small portion of the interstitial connective tissue, the location of TG in the lamprey was similar to that in the thyroid of the rat.
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    URL: http://dx.doi.org/10.1007/BF00229611
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    Penetration of spermatozoon into the ovum and transformation of the sperm nucleus into the male pronucleus in the domestic fowl, Gallus gallus (1978)
    Springer
    Cell & tissue research 190 (1978), S. 89-98 
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    ISSN: 1432-0878
    Keywords: Fertilization ; Membrane fusion ; Male pronucleus ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The apex of the sperm head which has undergone the acrosome reaction comes in contact with the plasma membrane of the ovum. After the entire surface of the inner acrosomal membrane has come into close contact with the plasma membrane of the ovum, the two membranes fuse to form a continuous membrane. All parts of the spermatozoon that are devoid of plasma membrane penetrate into the ooplasm. As the head of the spermatozoon moves deeper into the ooplasm, the chromatin begins to disperse, and the head of spermatozoon is transformed into a large spherical nucleus with low electron density. At a later stage of the transformation, many small vesicles appear around the nucleus and subsequently fuse to form two continuous membranes. These membranes represent the male pronuclear envelope. The condensation of the chromatin occurs in places in the nucleus, so that the male pronucleus is formed. During the course of the formation of the male pronucleus, the subacrosomal rod and tail become detached from the head and disintegrate.
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    URL: http://dx.doi.org/10.1007/BF00210039
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    Immunocytochemical study of the neurohypophysial hormone producing system of the lungfish, Protopterus aethiopicus (1978)
    Springer
    Cell & tissue research 190 (1978), S. 69-77 
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    ISSN: 1432-0878
    Keywords: Hypothalamus ; Dipnoi (Protopterus) ; Neurohypophysial hormones ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Using the unlabeled antibody peroxidase-antiperoxidase complex (PAP) technique at the light microscopic level, it has been shown that, in the dipnoan preoptico-hypophysial neurosecretory system, vasotocin and mesotocin are synthesized in separate neurons. In the preoptic nuclei, the perikarya of these two types of neurosecretory neurons are not located preferentially. The two types of neurosecretory perikarya give rise to separate vasotocinergic and mesotocinergic axons, respectively. The dipnoan median eminence and neural lobe contain separate vasotocinergic and mesotocinergic nerve fibres, the general distribution of which is described. In the pars distalis and the pars intermedia of the hypophysis, neurohypophysial hormone-containing nerve fibres have not been found.
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    URL: http://dx.doi.org/10.1007/BF00210037
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    On the ampullary organs of the South-American paddle-fish Sorubim lima (Siluroidea, Pimelodidae) (1978)
    Springer
    Cell & tissue research 190 (1978), S. 357-369 
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    ISSN: 1432-0878
    Keywords: Ampullary organs ; Siluroidea ; Sensory cells ; Synaptic junctions ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Ampullary organs were found in the epidermis of the paddle-fish Sorubim lima; they are distributed all over the skin surface of the fish but are particularly densely grouped in the head region and on the dorsal surface of the paddle. Histological and electron microscopical observations show that their structure is similar to the type of cutaneous ampullary organs characteristic of other Siluroidea. Composed of a relatively large mucus-filled ampulla, the organ possesses a short and narrow canal which leads to the outer epidermal surface. The wall of the ampulla is formed of several layers of flat epidermal cells. In general four sensory cells, each one surrounded by supporting cells, compose the sensory epithelium at the bottom of the ampulla. The inner surface of the sensory cells in contact with the ampullary mucus bears only microvilli. The contact between the nerve endings and the sensory cells show the characteristic structure of an afferent neuro-sensory junction. Two ampullae are innervated in some cases by the same afferent nerve fibre.
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    URL: http://dx.doi.org/10.1007/BF00218181
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    The ultrastructure of the male and female prostate of Praomys (Mastomys) natalensis (1978)
    Springer
    Cell & tissue research 190 (1978), S. 539-552 
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    ISSN: 1432-0878
    Keywords: Prostate ; Praomys natalensis ; Castration ; Changes ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Male ventral and female prostates of Praomys (Mastomys) natalensis were examined with the electron microscope. The findings support and add to information obtained with the light microscope on tissues from normal, castrated and ovariectomised animals. Our results indicate that although the female prostate may be considered a homologue of the male ventral prostate anatomically and histologically, there are differences in sub-cellular morphology and hormone dependence. Cells of the intact ventral prostate of the male are characterised by prominent dilated Golgi vesicles and electron-dense “mature secretory granules” seen in the apical region of the cell. In the cells of the female prostate these features are absent. These morphological differences reflect the influence of hormones upon the cells, as shown by the reduction of the dilated Golgi vesicles in the castrated male and conversely, their occasional presence in the cells of the oestrous female. Comparison of castrated and ovariectomised animals shows that the male ventral prostate is much more dependent on androgens than the female is on ovarian hormones. There are several modes of secretion in the male ventral and the female prostate. These are by acellular and cellular blebbing, by a variety of secretory vesicles into the acinar lumina, and by a system of “double walled” vesicles not previously described.
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    URL: http://dx.doi.org/10.1007/BF00219563
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    On the chromaffin cells in dog adrenal medulla; with special reference to the small granule chromaffin cells (SGC cells) (1978)
    Springer
    Cell & tissue research 191 (1978), S. 1-14 
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    ISSN: 1432-0878
    Keywords: Adrenal medulla ; Dog ; SGC cell ; Histochemistry ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Small granule chromaffin cells (SGC cells) were identified in the adrenal medulla of adult dogs. They were small in size and usually showed a high nucleo-cytoplasmic ratio. Cytoplasmic projections were occasionally observed in some of these cells. They contained a variable number of small secretory granules with diameters ranging from 70 to 300 nm, but mostly from 100 to 200 nm. The densities of the secretory granules were variable, ranging from highly dense to less dense. These adrenal SGC cells were rich in free ribosomes and polysomes, but were relatively poor in other cell organelles. Chromaffin cells which were intermediate in their characteristics (IM cells) between the SGC cells and the typical A and N cells were also identified. These IM cells contained both highly electron dense and less dense granules in various proportions. The IM cells were classified into two subgroups, according to the proportions of adrenaline type granules and noradrenaline type granules. One group resembled A cells (IM-A cells) and the other resembled N cells (IM-N cells). Light microscopic histochemical studies of A cells stained with the ammoniacal silver solution demonstrated that they contained a small number of darkly stained granules. Electron microscopic cytochemistry revealed that the electron dense granules in the SGC cells, IM cells and A cells reacted positively with both the potassium dichromate solution at pH 4.1 and the ammoniacal silver solution.
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    The spermatozoon of Oikopleura dioica Fol (Larvacea, Tunicata) (1978)
    Springer
    Cell & tissue research 191 (1978), S. 27-37 
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    ISSN: 1432-0878
    Keywords: Spermatozoon ; Oikopleura dioica ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The spermatozoon of Oikopleura dioica is about 30 μm long, with a spherical head, about 1 μm wide, a 3 μm long and 1 μm wide midpiece, and a 25 μm long tail with a tapered end piece. The head contains a nucleus with the chromatin volume limited to about 0.1 μm3. A small acrosome is found in an anterior inpocketing, and a flagellar basal body in a posterior inpocketing of the nucleus. The midpiece contains a single mitochondrion with the flagellar axoneme embedded in a groove along its medial surface. The flagellar axoneme has the typical 9 + 2 substructure, and the basal body the typical 9+0 substructure. A second centriole and special anchoring fibres are absent.
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    URL: http://dx.doi.org/10.1007/BF00223213
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    Effects of systemic administration of 6-hydroxydopamine on the circumventricular organs in nonhuman primates (1978)
    Springer
    Cell & tissue research 191 (1978), S. 141-150 
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    ISSN: 1432-0878
    Keywords: Subfornical organ ; Third ventricle ; 6-Hydroxydopamine ; Electron microscopy ; Primate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The neurotoxin 6-hydroxydopamine (6-OH-DA) has been shown to produces degenerative changes in noradrenergic nerve terminals and preterminals in the CNS following intracisternal, intraventricular, and stereotaxic injection into the brain parenchyma. Systemic injections of this drug are also known to result in degenerative changes in noradrenergic terminals in the peripheral nervous system and in the circumventricular organs (CVO; areas of the CNS which lie outside the blood brain barrier). In the present study eight adult female cynomolgus monkeys were employed. The four experimental animals were injected on two successive days with 150 and 200 mg/kg 6-OH-DA, respectively. The four controls received only the diluent consisting of 0.1% ascorbic acid in normal saline. Two animals from each of the experimental and control groups were sacrificed at 2 h and 24 h after the second injection. Degenerative changes in the SFO neurons were characterized by a generalized increase in electron density of cytoplasmic elements in axonal terminals and preterminals. Multilamellar bodies, and increases in the number of dense core vesicles, dense bodies and secondary lysosomes were also observed after treatment with 6-OH-DA. The neurons showed clumping of mitochondria, which also appeared to be undergoing degenerative changes. The vacuoles in some supraependymal cells were greatly dilated as was the Golgi apparatus in the ependymal cells. The ependymal cell layer appeared to be intact, but there were areas immediately deep to this cell layer that contained large extracellular spaces. This increase in extracellular space was also commonly observed surrounding the perivascular spaces. These phenomena greatly contribute to the “spongy” appearance that the SFO takes on after 6-OH-DA administration.
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    URL: http://dx.doi.org/10.1007/BF00223222
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    The frontal ganglion of Periplaneta americana L. (insecta) (1978)
    Springer
    Cell & tissue research 191 (1978), S. 171-182 
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    ISSN: 1432-0878
    Keywords: Frontal ganglion ; Periplaneta ; Synaptic contacts ; Immunohistochemistry ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The frontal ganglion, part of the stomatogastric nervous system, contains about 60 to 80 neurons, 25 to 30 μm in diameter. A well developed Golgi system, producing dense-core vesicles, lysosomes, multivesicular bodies and dense bodies are abundant. Glia elements are sparsely distributed. Many nerve fibres contain granules of different size and electron density. Five groups of fibres can be distinguished: Fibres with granules of about 200 nm (type A), fibres with granules of about 160 to 170 nm (type B), fibres with granules of about 80 to 100 nm (type C) and those with synaptic vesicles of 50 nm (type D) respectively. A fifth very small type contains neither vesicles nor granules. Special attention was paid to synaptic contacts. The divergent dyad seems to be the main type in the frontal ganglion. Frequently, neurosecretory endings are observed in presynaptic position. Immunocytochemical “staining” of neurosecretory material closely corresponds to the distribution of type A fibres, as observed electron microscopically. Immunoelectrophoresis of extracts from frontal ganglia with polyspecific anti-neurosecretion-serum reveals a single precipitation line, indicating that the immunocytochemical localization of neurosecretory material is due to reaction with a specific as well as a crossreagent antibody.
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    URL: http://dx.doi.org/10.1007/BF00223225
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    Effect of cyproterone acetate, d-norgestrel and progesterone on cells of the pars distalis of the adenohypophysis in the beagle bitch (1978)
    Springer
    Cell & tissue research 191 (1978), S. 205-218 
    Details
    ISSN: 1432-0878
    Keywords: Cyproterone acetate ; d-Norgestrel ; Progesterone ; Pars distalis ; adenohypophysis ; Immunocytochemistry ; Dog (Beagle)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The effects of short-term (8 weeks) treatment with different doses of cyproterone acetate (CPA), d-norgestrel (d-N) and progesterone on cells of the pars distalis, as revealed by the immunoperoxidase technique, were studied in cycle-synchronized beagle bitches (first anoestrus). Pituitary glands from non-treated primiparous beagle bitches at the 6th and 9th week of pregnancy were also included. For immunochemical staining specific antisera to the following hormones were used: canine GH, canine PRL, porcine ACTH, bovine TSHβ, bovine LHβ and human FSHβ. Morphological features of high secretory activity in GH cells were evident even after the human oral contraceptive doses of CPA and d-N, and after a dose as low as 0.1 mg/kg/day subcutaneously (s.c.) of progesterone. In contrast, PRL cells did not show any significant treatment-related effects except in those animals which received the highest dose of d-N (0.5 mg/kg/day per os). In this group, as well as in all pregnant bitches, hyperplasia and hypertrophy of PRL cells were found. In the animals treated with the highest doses of CPA (4.0 mg/kg/day per os) and progesterone (42.5 mg/kg/day s.c.) as well as in pregnant bitches, ACTH/MSH and TSH cells showed marked atrophy and regressive changes. Similar morphological signs of depressed secretory activity were also observed in the cells shown to contain FSHβ and/or LHβ as a result of treatment with the highest dose of progesterone and at the 9th week of pregnancy. These structural responses indicate that quantitative and/or qualitative differences may exist between progesterone, the synthetic progesterone derivative CPA and the nortestosterone type progestagen d-N with regard to their effect on pituitary hormone secretion in the beagle bitch.
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    URL: http://dx.doi.org/10.1007/BF00222420
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    Effects of antimicrotubular agents on the fine structure of the Golgi complex in embryonic chick osteoblasts (1978)
    Springer
    Cell & tissue research 193 (1978), S. 247-257 
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    ISSN: 1432-0878
    Keywords: Osteoblasts (Chick embryo) ; Golgi complex ; Microtubules ; Colchicine ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Embryonic chick frontal bones were cultured in the presence of colchicine or vinblastine and subsequently examined by transmission electron microscopy. In control cultures the osteoblasts showed a large Golgi complex consisting of dictyosomes arranged in a well-defined juxtanuclear area. Microtubules were particularly numerous within this Golgi area although they could be observed throughout the cytoplasm. Colchicine and vinblastine caused the disappearance of cytoplasmic microtubules, while bundles of 10nm diameter filaments appeared more frequently. In addition, cell polarity was lost and the Golgi complex became disorganized, with the dictyosomes randomly dispersed in the cytoplasm and showing a decreased number of cisternae and an increased number of vacuoles, the latter generally lacking stainable material. Increased number of autophagosomes were also noted. These findings indicate that microtubules function in the organization of the Golgi complex in osteoblasts. In view of the well documented role of this organelle system in collagen secretion it is suggested that previously observed secretory disturbances produced by antimicrotubular drugs may be due to a defective transfer of material to the dictyosomes and/or a defect in the packaging and transport of such material away from them.
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    URL: http://dx.doi.org/10.1007/BF00209038
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    Ultrastructural and cytochemical studies on the cytodifferentiation of duodenal endocrine cells (1978)
    Springer
    Cell & tissue research 194 (1978), S. 79-102 
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    ISSN: 1432-0878
    Keywords: Duodenum ; Endocrine cells ; Differentiation ; Immunocytochemistry ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The development and cytodifferentiation of endocrine cells that produce the gastrointestinal hormones gastrin, cholecystokinin and secretin have been studied by a combined fluorescence-cytochemical, immunocytochemical and ultrastructural approach. The results show that, during development, several ultrastructurally distinct cell types exhibit COOH-terminal gastrin and cholecystokinin immunoreactivity. Furthermore, some cells simultaneously contain both gastrin- and cholecystokinin-specific antigenic determinants. Studies on the time course of development of gastrin and cholecystokinin cells, together with the above-mentioned data, suggest that gastrin cells may be converted into cholecystokinin cells in development. During this period, gastrin, cholecystokinin and secretin cells store the biogenic monoamine, 5-hydroxytryptamine a feature not displayed by the adult counter-parts of these cells. In the adult duodenum, characteristic enterochromaffin (EC) cells store 5-hydroxytryptamin for which, evidence for a possible hormonal role has been presented. Taken together, our data indicate that the differentiation of duodenal endocrine cells occurs in distinct steps, each involving a restriction in the biosynthetic repertoire of the cell.
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    URL: http://dx.doi.org/10.1007/BF00209235
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    The ultrastructure of guinea pig heterophil granulocytes and the heterogeneity of the granules (1978)
    Springer
    Cell & tissue research 194 (1978), S. 183-205 
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    ISSN: 1432-0878
    Keywords: Bone marrow ; Heterophil granulocytes ; Granules ; Guineapig ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The development of the heterophil granulocytes in the bone marrow of the guinea pig is described. During the maturation of these cells, three types of granule are formed, not only the azurophil and specific granules already described in other mammals but also a third type of granule referred to here as the nucleated granule. During the process of maturation of the cells, these three types of granule are formed successively. On this basis, two steps can be distinguished in the promyelocyte phase in which primary (nucleated and azurophil) granules are formed, i.e. an early and a late stage, nucleated granules being formed in early and azurophil granules in late promyelocytes. Secondary (specific) granules occur first in myelocytes. In mature heterophils of the guinea pig the granule population is composed of about 85% secondary granules, about 10% azurophil granules, and about 5% nucleated granules. The changes in the granule population during the maturation process were quantified. The observations and calculations point to the occurrence of three mitoses: one in the early and one in the late promyelocyte and the third in the myelocyte.
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    URL: http://dx.doi.org/10.1007/BF00220388
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    Cytochalasin B-induced changes in concanavalin A-activated lymphocytes (1978)
    Springer
    Cell & tissue research 194 (1978), S. 279-285 
    Details
    ISSN: 1432-0878
    Keywords: 14C-thymidine incorporation ; Autoradiography ; Multinucleated blasts ; Electron microscopy ; Deformities of cellular membranes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Cytochalasin B (CB) administered simultaneously with a mitogenic dose of concanavalin A (Con A) interferes with the activation process. This interference involves structural alterations of cellular membrane which do not include a reduced Con A-binding capacity. This conclusion is supported by the observation of deformities in both nuclear and cytoplasmic membranes in Con A-activated lymphocytes subsequently treated with CB. The high incidence of membrane blebs and pseudomyelin bodies in the cytoplasm points to a general effect of CB on the structural organization of membrane which may secondarily interfere with some specific event such as generation or transfer of signals for activation or cytokinesis.
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    URL: http://dx.doi.org/10.1007/BF00220394
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    Distribution, ontogeny and ultrastructure of pancreatic polypeptide (PP) cells in the pancreas and gut of the chicken (1978)
    Springer
    Cell & tissue research 194 (1978), S. 377-386 
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    ISSN: 1432-0878
    Keywords: Pancreas ; Gut ; Pancreatic polypeptide (PP) cells ; Immunocytochemistry ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Pancreatic polypeptide (PP) cells in the pancreas and gut of chickens were studied by immunocytochemistry. In the pancreas PP cells were numerous and disseminated in the exocrine parenchyma. In this location they were first seen at the 9th day of incubation, i.e. several days after the appearance of glucagon, insulin and somatostatin cells. Very large numbers of these cells occurred from about the 14th day until shortly after hatching when the PP cell frequency was somewhat reduced. At the 17th day of incubation PP cells appeared in the duodenum. Subsequently the number of PP cells in the duodenum increased, and PP cells began to appear also in the jejunum-ileum (19th day) and in the proventriculus and colon (21st day). At hatching and a few days thereafter, PP cells were relatively numerous in the small intestines but much less frequent in the proventriculus and colon. One week after hatching PP cells had disappeared from the colon but remained in the proventriculus and small intestines. Ultrastructurally the PP cell was clearly distinguishable from the insulin, glucagon and somatostatin cells. It was characterized by the presence of spherical cytoplasmic granules which were membrane-bound and moderately electron dense. Areas of firm adhesion between PP cells and acinar cells in the form of desmosomes and possibly also gap junctions were observed.
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    URL: http://dx.doi.org/10.1007/BF00236159
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    Identification by immunofluorescence of prolactin-and somatotropin-producing cells in the pituitary gland of the Mexican axolotl (Ambystoma mexicanum, Shaw) (1978)
    Springer
    Cell & tissue research 194 (1978), S. 497-501 
    Details
    ISSN: 1432-0878
    Keywords: Pituitary gland ; Mexican axolotl ; Prolactin ; Somatotropin ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The indirect immunofluorescence procedure was used to identify prolactin (LTH)-and somatotropin (STH)-producing cells in the pituitary of the Mexican axolotl. Histological staining techniques were employed to corroborate immunocytological results. The LTH cells are large, orange-staining cells (acidophils 1) distributed in the posterior two-thirds of the pars distalis. The STH cells are small, erythrosinophilic elements (acidophils 2) principally concentrated in the dorsal part of the pars distalis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00236168
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  • 94
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    Presynaptic and postsynaptic organelles of synapses formed in cultures of previously dissociated mouse spinal cord (1978)
    Springer
    Cell & tissue research 194 (1978), S. 503-511 
    Details
    ISSN: 1432-0878
    Keywords: Cell cultures ; Electron microscopy ; Synaptic organelles ; Spinal cord (mouse)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary This study describes some of the ultrastructural features of presynaptic and postsynaptic organelles at synapses developed in cultures of previously dissociated mouse spinal cord cells. Particular attention was paid to the agranular reticulum which is well developed at many presynaptic and postsynaptic sites, either in the form of simple tubules or cisternae, or more complex networks and often closely associated with mitochondria. In addition, the disposition of microtubules at and close to synaptic specializations is described. These and other features of synaptic zones, such as granular vesicles in presynaptic sites, are discussed in relation to cultures developed on feeder layers and synapses in vivo, and in relations to possible degenerative and regenerative events in the cell cultures.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00236169
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  • 95
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    Myosatellite cells associated with different muscle fibre types in the Atlantic hagfish (Myxine glutinosa, L.) (1978)
    Springer
    Cell & tissue research 195 (1978), S. 17-27 
    Details
    ISSN: 1432-0878
    Keywords: Myosatellite cells ; Muscles ; Atlantic hagfish (Myxine glutinosa, L.) ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The incidence of myosatellite cells associated with “white” and “red” muscle fibres of the parietal muscle and “red” fibres of the craniovelar muscle was estimated by quantitative electron microscopy in the Atlantic hagfish (Myxine glutinosa, L.). Myosatellite cell nuclei constitute 3, 11 and 23 % of the total number of nuclei inside the basal lamina of the three types of muscle fibres, respectively. However, the total number of nuclei is highest in “white” fibres, most of the nuclei belonging to striated muscle cells. Myosatellite cell profiles in transverse sections constitute 23, 41 and 61 % of the number of muscle fibre profiles of the three types, respectively. The intervals between adjacent myosatellite cells are ∼ 135 μm in “white” fibres, ∼ 55 μm in “red” parietal fibres, and only ∼ 25 μm in craniovelar fibres. Since craniovelar fibres are also comparatively thin, myosatellite cells constitute a significant fraction of the volume inside the basal lamina in these fibres. The myosatellite cells are ∼ 30–50 μm long and up to 5 μm thick. Some myosatellite cells possess few organelles, whereas others appear to contain many free ribosomes, granular endoplasmic reticulum, prominent Golgi apparatus and lysosome-like bodies.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00233674
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  • 96
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    Ultrastructural localization of radiolabelled L-dopa in the endocrine hypothalamus of the rat (1978)
    Springer
    Cell & tissue research 195 (1978), S. 29-43 
    Details
    ISSN: 1432-0878
    Keywords: Endocrine hypothalamus (rat) ; Arcuate nucleus ; L-dopa ; Autoradiography ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Light and electron microscopic autoradiography has been employed to define the neuroanatomical patterns of uptake and binding of radiolabelled L-dopa in the endocrine hypothalamus of the rat. A dorsomedial continuum of arcuate and periventricular neurons selectively sequester 3H L-dopa 20 min following its intraventricular infusion. By 40 and 60 min following the infusion labelling of neurons is minimal and supports the notion of rapid degradation. Other cell compartments such as tanycytes demonstrate uptake of 3H L-dopa. The ultrastructural localization and distribution of radiolabelled L-dopa (or its metabolites) in the rodent hypothalamus is discussed with respect to mechanisms and cell compartments involved in neuroendocrine regulatory processes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00233675
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  • 97
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    Ultrastructural evidence for adrenergic nerve degeneration in the guinea pig uterus during pregnancy (1978)
    Springer
    Cell & tissue research 195 (1978), S. 189-193 
    Details
    ISSN: 1432-0878
    Keywords: Guinea pig uterus ; Myometrium, pregnancy ; Sympathetic nerves ; Electron microscopy ; Axon degeneration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In the guinea pig myometrium, the adrenergic nerves selectively demonstrated at the ultrastructural level after treatment with 5-OH-DA, show varying degree of degeneration during pregnancy. The changes are more extensive in a late gestational stage (40–45 days) than in an early one (20–25 days), and are particularly evident in the uterus overlying the conceptus as compared to the regions between the fetuses. Scattered degenerative changes were also observed in myometrial specimens from virgin animals, but probably reflect the normal continuous turnover of axons.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00233686
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  • 98
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    Non-ependymal cilia in the habenulae and the interpeduncular nucleus of the frog tadpole (1978)
    Springer
    Cell & tissue research 195 (1978), S. 527-533 
    Details
    ISSN: 1432-0878
    Keywords: Ciliated neurons ; Habenula ; Interpeduncular nucleus ; Electron microscopy ; Rana esculenta, tadpole
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Cilia of the 9+2 pattern are found electron microscopically in nonependymal cells of the habenulae and the interpeduncular nucleus of the tadpole of Rana esculenta at an early stage of development (8 mm length, head to tip of tail). A comparison is made between these and the ependymal and sensory cilia in the same specimens. The cilia project into the neuropil emerging from a perikaryon rich in free ribosomes and displaying a prominent Golgi apparatus. These perikarya contain dense core vesicles. Synapses with vesicles of the clear spherical type have been observed along the ciliary shaft. On a purely morphologic basis the authors hypothesize that these cilia, at least in this early ontogenetic stage, may extend considerably the conducting surface of the cell and represent a sensory structure which could be stimulated by terminal processes belonging to distantly located cells. In addition, they could also be involved in the trophic exchange of material with the adjacent structures.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00233894
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  • 99
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    Electronic Resource
    Phagocytosis of osteocytes by osteoclasts in femora of two week-old rabbits (1978)
    Springer
    Cell & tissue research 195 (1978), S. 557-564 
    Details
    ISSN: 1432-0878
    Keywords: Osteoclasts ; Osteocytes ; Bone resorption ; Phagocytosis ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The osteoclast-osteocyte relationship at the endosteal surface of femora of two-week old rabbits was studied. Light microscopic observations suggest that during physiological resorption phagocytosis by osteoclasts of osteocytes takes place. Serial sections confirm that the cells are totally engulfed within the cytoplasm of the osteoclasts. Ultrastructural studies support these findings and indicate that the initial stage of phagocytosis of the osteocytes consists of the insinuation of an extension of the ruffled border into the osteocyte lacuna. These extensions are seen to make close contact with the osteocytes prior to their engulfment by the osteoclasts and their final digestion within phagosomes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00233897
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  • 100
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    The fine structural localization of acetylcholinesterase in the muscle spindle of the rat (1978)
    Springer
    Cell & tissue research 186 (1978), S. 39-52 
    Details
    ISSN: 1432-0878
    Keywords: Muscle spindle ; Nerve endings ; Acetylcholinesterase ; Cytochemistry ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Muscle spindles from lumbricalis muscles of the rat were incubated for acetylcholinesterase with a modified thiocholine-method of Lewis and Shute and examined by light and electron microscopy. All types of motor nerve ending showed heavy deposits of reaction product in the synaptic cleft. The underlying sarcoplasmic reticulum, transverse tubular system, and, when present, the envelope of sole plate nuclei were also stained. In the sensory region, the reaction was negative in the interface between the plasma membranes of the primary sensory terminal and muscle. One of two secondary sensory endings identified showed distinct reaction product in the cleft; the other secondary sensory ending showed no such reaction. Precipitates were present on the sarcolemma of the intrafusal muscle fibers in the polar and adjacent myotube regions, but not at the spindle equator. Extrafusal and intrafusal myelinated γ-nerve fibers and preterminal motor axons showed staining of the axolemma. Fibers with thick myelin sheaths and preterminal sensory axons were free of acetylcholinesterase activity, as were the unmyelinated nerve fibers.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00219653
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