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  • Articles  (15)
  • Phaseolus  (15)
  • Springer  (15)
  • American Institute of Physics
  • Cell Press
  • Sage Publications
  • 2010-2014
  • 2005-2009
  • 1985-1989
  • 1975-1979  (15)
  • 1945-1949
  • 2007
  • 2005
  • 1978  (15)
  • 1945
  • Biology  (15)
  • Computer Science
  • Electrical Engineering, Measurement and Control Technology
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  • Articles  (15)
Source
Keywords
Publisher
  • Springer  (15)
  • American Institute of Physics
  • Cell Press
  • Sage Publications
Years
  • 2010-2014
  • 2005-2009
  • 1985-1989
  • 1975-1979  (15)
  • 1945-1949
Year
Topic
  • Biology  (15)
  • Computer Science
  • Electrical Engineering, Measurement and Control Technology
  • 1
    Electronic Resource
    Electronic Resource
    Early time course and specificity of auxin effects on turgor movement of the bean pulvinus (1978)
    Springer
    Planta 140 (1978), S. 107-109 
    Details
    ISSN: 1432-2048
    Keywords: Auxin ; Leaf movement ; Phaseolus ; Pulvinus ; Turgor movement
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Auxin application to the upper side of the pulvinus of primary leaves of Phaseolus vulgaris L. promoted bending away from the place of application. The effect had a latency of less than 20 min and was specifically induced by substances known as active auxins in growth tests (indoleacetic and 1-naphthaleneacetic acid) but not by inactive auxin analogs (2-naphthaleneacetic, 3-indolepropionic and benzoic acid); 2,4-dichlorophenoxyacetic acid, and L-(-)-2,4-dichlorophenoxyisopropionic acid were of intermediate activity. Auxin-promoted bending was reversible and presumably caused by turgor increase in the treated cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00384908
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  • 2
    Electronic Resource
    Electronic Resource
    Photosynthetic apparatus in chilling-sensitive plants (1978)
    Springer
    Planta 140 (1978), S. 121-128 
    Details
    ISSN: 1432-2048
    Keywords: Chilling ; Cold storage ; Cucumis ; Hill reaction ; Lycopersicon ; Phaseolus ; Photosynthesis ; Temperature (chilling)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Chloroplast isolated from the detached leaves of chilling-sensitive plants-Phaseolus vulgaris L., Cucumis sativus L., and Lycopersicon esculentum Mill.-stored in the cold for 2–4 days in the dark exhibit an almost complete loss of Hill reaction activity, which on illumination of leaves is restored to almost the original level. In contrast, illumination of either chloroplast suspensions or homogenates from leaves stored in the cold in the dark does not cause restoration of electron transport. Cold and dark storage of leaves of chilling-sensitive plants affects the electron transport before the site of electron donation by diphenylcarbazide and results in an increased sensitivity of the Hill reaction of isolated chloroplasts to exogenous linolenic acid. Illumination of leaves reverses these processes. When tomato plants are exposed to 0°C in intermittent light, Hill reaction activity is not affected while dark storage either at 0°C or 25°C results in a significant decrease of Hill reaction activity after 2–3 days followed by the restoration of electron transport to the original level after 1 or 2 days of the prolonged dark storage of plants. When tomato plants are stored either at 0°C in intermittent light, at 0°C in dark, or at 25°C in dark the sensitivity of the Hill reaction to exogenous linolenic acid remains increased despite a significant restoration of this activity. In conclusion, both darkness and the detachment of leaves from the plant are more effective than cold treatment in damaging photosystem II whereas both light and intact structure of the cell are required for restoration of Hill reaction activity in chloroplasts following cold and dark storage of detached leaves.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00384910
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  • 3
    Electronic Resource
    Electronic Resource
    Distorted phytochrome action spectra in green plants (1978)
    Springer
    Planta 138 (1978), S. 25-28 
    Details
    ISSN: 1432-2048
    Keywords: Action spectrum ; Chlorophyll ; Phaseolus ; Photomorphogenesis ; Phytochrome
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Red light absorption by photosynthetic pigments screens stem tissue of Phaseolus vulgaris L. seedlings to such an extent that the most effective wavelength for the phytochrome induction control of stem growth is approximately 628 nm. Screening is greater in the first internode than in the hypocotyl. The consequences are that, compared with etiolated seedlings, much higher irradiances are required for phytochrome control in green plants, and the efficiency of 660 nm light is markedly reduced. Even very high exposures at 660 nm may not achieve appreciable photoconversion of P r to P fr.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00392909
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  • 4
    Electronic Resource
    Electronic Resource
    Dihydrozeatin riboside, a minor cytokinin from the leaves of Phaseolus vulgaris L. (1978)
    Springer
    Planta 140 (1978), S. 151-153 
    Details
    ISSN: 1432-2048
    Keywords: Cytokinin ; Leaves ; Phaseolus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Dihydrozeatin riboside has been identified in the leaves of decapitated bean plants by Sephadex LH20 chromatography and combined gas chromatography-mass spectrometry. The relationship between the cytokinins isolated and identified from this system and those previously reported in Phaseolus is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00384914
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  • 5
    Electronic Resource
    Electronic Resource
    Stimulation of volume flow and ion flux by abscisic acid in excised root systems of Phaseolus vulgaris L. cv. Redland Pioneer (1978)
    Springer
    Planta 141 (1978), S. 37-43 
    Details
    ISSN: 1432-2048
    Keywords: Abscisic acid ; Ion flux ; Phaseolus ; Root (ion transport) ; Transport (ions) ; Xylem (ion transport)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Abscisic acid (ABA) caused a 7–8-fold increase in volume flow in excised bean root systems and this was coupled with an increase in 42K, 36Cl and 24Na flux into the xylem. The transport of 42K and 36Cl increased by a factor larger than the stimulation of volume flow, resulting in an increase in the concentration of those ions in the xylem exudate. Carbonyclcyanide-m-chlorophenyl hydrazone, on the other hand, eliminated ABA-stimulated 42K transport and caused a further inhibition of 42K flux, thus providing additional support for the proposition that ABA stimulation may involve an energised process of ion transport. ABA also increased the accumulation of 24Na and 36Cl in bean root tissue, but not that of 42K.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00387742
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  • 6
    Electronic Resource
    Electronic Resource
    Comparison of the in vitro translation products of mRNA isolated from suspension cultures of Phaseolus vulgaris grown on maintenance and induction medium (1978)
    Springer
    Planta 138 (1978), S. 41-48 
    Details
    ISSN: 1432-2048
    Keywords: Cell suspension cultures ; Differentiation ; mRNA ; Phaseolus ; Reticulocyte-lysate ; Translation (mRNA) ; Wheat-germ
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract 1. Bean cells grown in suspension culture on maintenance medium have been shown to differentiate (xylem and phloem production) when they are transferred to an induction medium containing an increased ratio of naphthylacetic acid to kinetin.-2. Conditions for the extraction and isolation of undegraded mRNA are described. The mRNA has been translated by 2 in vitro protein synthesizing systems; the wheat germ and the mRNA-dependent-reticulocyte-lysate preparations.-3. A greater proportion of the mRNA from cells on maintenance medium coded for high molecular weight products compared with that from cells on induction medium.-4. mRNA from cells on induction medium coded for a relatively large amount of a few polypeptides whereas that from cells on maintenance medium coded for a large number of polypeptides all present in fairly equal amounts.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00392912
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  • 7
    Electronic Resource
    Electronic Resource
    The activity of Triton X-100 soluble chlorophyllase in liposomes (1978)
    Springer
    Planta 140 (1978), S. 75-80 
    Details
    ISSN: 1432-2048
    Keywords: Chlorophyllase ; Chlorophyll a biosynthesis ; Liposomes ; Phaseolus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Chlorophyllase (chlorophyll-chlorophyllidohydrolase, EC 3.1.1.14) was isolated and purified from Phaseolus vulgaris L. chloroplasts and etioplasts dissolved in 1% Triton X-100 and 10% glycerol. A 100 and 40-fold purification, respectively, was achieved. Enzyme preparations from both sources had similar affinities for chlorophyll a when assayed in a Triton X-100 medium. When electrophoresed in sodium dodecyl sulphate polyacrylamide gels the major band in both preparations migrated as a peptide of 30,000 daltons. Chlorophyll containing liposomes were also used as a substrate for chlorophyllase. The rate of hydrolysis did not follow Michaelis-Menten kinetics. When chlorophyllide a or methyl chlorophyllide a was incorporated in the liposomes, then in the presence of phytol dissolved in methanol, methylchlorophyllide a and chlorophyll a were shown to be synthesized. Apparently the purified enzyme in the presence of lipids, is endowed with both synthetic and hydrolytic activity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00389383
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  • 8
    Electronic Resource
    Electronic Resource
    Photosynthetic apparatus in chilling-sensitive plants (1978)
    Springer
    Planta 140 (1978), S. 129-136 
    Details
    ISSN: 1432-2048
    Keywords: Chilling ; Chloroplasts ; Cold storage ; Cucumis ; Lycopersicon ; Phaseolus ; Photosynthesis ; Temperature (chilling)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The composition of free fatty acids (FFA) in relation to Hill reaction activity and photoperoxidation of lipids was studied in chloroplasts isolated from fresh, cold and dark-stored as well as illuminated leaves of Lycopersicon esculentum Mill., Phaseolus vulgaris L. and Cucumis sativus L. Following the cold and dark-storage of leaves the loss of Hill reaction activity is accompanied by approximately a 5-fold increase in the amount of FFA and by an increase in the percentage of unsaturated FFA, particularly that of linolenic acid. Illumination of the cold- and dark-stored leaves restores both Hill reaction activity and the content and composition of chloroplast FFA. Following the second and third cycles of cold storage and illumination of leaves the percentage of unsaturated fatty acids in chloroplasts increases while that of saturated ones decreases despite of the significant restoration of Hill reaction activity. Since the illumination of cold-stored leaves results in peroxidation of inhibitory fatty acids it seems likely that this phenomenon could, at least partially, be responsible for the restoration of Hill reaction activity. Inhibition of Hill reaction activity by exogenous linolenic acid in chloroplasts of fresh, cold-stored as well as cold-stored and illuminated leaves could be reversed following the incubation of chloroplast suspension with BSA, however only to a value measured in the absence of unsaturated fatty acid. All these results indicate that the inhibition of Hill reaction activity due to the cold and dark storage of leaves is caused by both inhibitory FFA released from chloroplast lipids as well as by damage to the thylakoid structure affecting the electron transport within photosystem II.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00384911
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  • 9
    Electronic Resource
    Electronic Resource
    Phytochrome involvement in the induction of resistance to dark abscission by malformin (1978)
    Springer
    Planta 141 (1978), S. 311-314 
    Details
    ISSN: 1432-2048
    Keywords: Abscission resistance ; Malformin ; Phaseolus ; Phytochrome
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The active portion of the visible spectrum which is required for malformin to produce leaves which are resistant to dark abscission from cuttings of Phaseolus aureus is red light. Abscission resistance was partially to almost completely lost by far irradiation prior to dark incubation. Although Ethrel, an ethylene releasing compound, stimulated dark abscission of resistant and control leaves, resistance was not lost because control leaves always abscised at a greater rate. The participation of phytochrome in the induction of abscission resistance by malformin is indicated.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00388349
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  • 10
    Electronic Resource
    Electronic Resource
    Characterization and subcellular localization of vicilin and phytohemagglutinin, the two major reserve proteins of Phaseolus vulgaris L. (1978)
    Springer
    Planta 142 (1978), S. 291-298 
    Details
    ISSN: 1432-2048
    Keywords: Cotyledons ; Phaseolus ; Phytohemagglutinin ; Protein (reserve) ; Seeds (protein) ; Vicilin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Extracts of bean (Phaseolus vulgaris L. cv. Greensleeves) cotyledons contained two abundant proteins: vicilin and phytohemagglutinin. Vicilin, a 6.9 S protein fraction at neutral pH, associated to an 18.0 S form at pH 4.5 and had 3 non-identical subunits with molecular weights (MW) of 52,000, 49,000 and 46,000. Phytohemagglutinin, a 6.4 S protein fraction, had 2 non-identical subunits with MW of 34,000 and 36,000. Phytohemagglutinin could be separated by isoelectrofocusing into a mitogenic and non-erythroagglutinating protein with a single subunit of MW=34,000, and a mitogenic and erythroagglutinating protein fraction which contained both subunits. Vicilin is apparently identical with the so called glycoprotein II (A. Pusztai and W.B. Watt, Biochim. Biophys. Acta 365, 57–71, 1970) and with globulin G1 (R.C. McLeester, T.C. Hall, S.M. Sun, F.A. Bliss, Phytochem. 2, 85; 1973), while phytohemagglutinin is identical with globulin G2 (McLeester et al., 1973). Since vicilin and phytohemagglutinin are internationally used names there is no need to introduce new names to describe P. vulgaris reserve proteins. Both proteins are catabolized in the course of seedling growth and are located in the protein bodies, indicating that they are reserve proteins. Vicilin isolated in its 18.0 S form from the cotyledons of young seedlings contains substantial quantities of smaller polypeptides, in addition the 3 original ones. We suggest that the presence of these small polypeptides represents partial breakdown of the vicilin prior to its complete catabolism.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00385080
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  • 11
    Electronic Resource
    Electronic Resource
    Embryo-suspensor relations in Phaseolus coccineus: cytokinins during seed development (1978)
    Springer
    Planta 143 (1978), S. 59-62 
    Details
    ISSN: 1432-2048
    Keywords: Cytokinins ; Embryo ; Phaseolus ; Seed development ; Suspensor
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Data are presented on the cytokinin status of seeds and seed components, at different stages of development in Phaseolus coccineus L., as determined with the soybean callus growth bioassay: A change in cytokinin types according to developmental stage occurred: from biologically very active less polar types (zeatin=Z) at early stages to more polar types (zeatin glucoside=Z9G and zeatin riboside=Zr), with relatively low biological activity, at intermediate and late stages of seed development: When cytokinins were analyzed separately in embryos (embryo proper) and suspensors at two embryonic stages: heart-shaped (A) and middle cotyledonary embryos (stage B) respectively, it was found that: i) at stage A, the suspensor showed cytokinin activity at the level of Z, 2iPA (2-isopentenyladenosine) and Zr, whereas more polar cytokinins (Z9G, Zr) were present in the embryo; ii) at stage B, when the embryo seems to become autonomous for cytokinin supply, there was a relative abundance of active cytokinins (Z, 2iPA) in the embryo to which Z9G activity in the suspensor corresponded. It is concluded that the suspensor plays an essential role in embryogenesis by acting as a hormone source to the early embryo.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00389052
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  • 12
    Electronic Resource
    Electronic Resource
    The estimation of sugar concentration in individual sieve-tube elements by negative staining (1978)
    Springer
    Planta 139 (1978), S. 19-24 
    Details
    ISSN: 1432-2048
    Keywords: Phaseolus ; Phloem ; Sieve tubes ; Sugar ; Translocation (phloem)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The presence of water-soluble compounds in sectioned plant tissue can be visualized in negative contrast by freeze-substitution in acetone, followed by embedment in Epon containing 6% Sudan B. The contents of mature sieve tubes and companion cells of bean (Phaseolus vulgaris L.) showed strong, and mostly uniform, negative staining. The degree of negative staining was measured by microspectrophotometry. Since, in sieve tubes, virtually all of the solute is the translocated sugar, the sugar concentration can be estimated by comparison with similar measurements made on sections from pith blocks which were infiltrated with sugar solutions and processed by the same procedures. Sieve tubes contained a solution of about 11.2% (w/v) sucrose; companion cells contained a similar concentration of sucrose. Negative staining, and therefore the sucrose concentration, in immature sieve-tube elements and companion cells was much less than in their mature counterparts.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00390804
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  • 13
    Electronic Resource
    Electronic Resource
    Levels of short-chain fatty acids and of abscisic acid in water-stressed and non-stressed leaves and their effects on stomata in epidermal strips and excised leaves (1978)
    Springer
    Planta 139 (1978), S. 281-287 
    Details
    ISSN: 1432-2048
    Keywords: Abscisic acid ; Beta ; Commelina ; Fatty acids ; Hordeum ; Phaseolus ; Stomata ; Water stress
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Straight-chain saturated fatty acids (C6-C11) and abscisic acid (ABA) accumulate in the leaves of Phaseolus vulgaris L. and Hordeum vulgare L. under water stress. ABA and certain of the fatty acids, particularly decanoic and undecanoic acid, can inhibit stomatal opening and cause stomatal closure in epidermal strips of Commelina communis L. depending on the incubating medium used. 10-4 M (±)-ABA inhibits opening in media containing either high or relatively low concentrations of KCl but causes closure only in the latter medium. The fatty acids (at 10-4 M) prevent opening in both media while significant closure of open stomata was caused only by undecanoic acid in both media and, additionally, by decanoic acid in the low-KCl medium. 10-4 M formic acid also caused stomatal closure and prevented opening to significant extents in the low-KCl medium (it was not tested in the high-KCl medium). The efficacy of undecanoic acid in causing 50% inhibition of opening is about three orders of magnitude lower than that of ABA. At a concentration of 10-3 M, nonanoic, decanoic and particularly undecanoic acid and all-trans-farnesol cause increased cell leakage in Beta vulgaris L. root tissue. Undecanoic acid (10-4 M) also causes some loss of guard cell integrity in C. communis within 1.5 h of treatment. ABA (10-4 M) reduces transpiration rates in barley and C. communis leaves when applied via the transpiration stream but decanoic and undecanoic acids did not have this effect. Transpiration was not affected when ABA or the fatty acids were applied to the leaf surfaces.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00388642
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  • 14
    Electronic Resource
    Electronic Resource
    Chlorophyllase activity in developing leaves of Phaseolus vulgaris L. (1978)
    Springer
    Planta 139 (1978), S. 79-83 
    Details
    ISSN: 1432-2048
    Keywords: Chlorophyllase ; Chlorophyll-proteins ; Etiolation ; Greening ; Phaseolus ; Plastid membranes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In crude extracts of primary leaves from dark grown seedlings of Phaseolus vulgaris L., relatively high hydrolytic activity of chlorophyllase (chlorophyll-chlorophyllido-hydrolase, EC 3.1.1.14) was observed. When plants were exposed to continuous illumination, the enzyme activity in the extracts was doubled within 3 days and both chlorophyll a and b were synthesized. However, when exposed to periodic illumination (1 min light-59 min dark) the enzyme activity was doubled within 1 to 2 days and chlorophyll a was synthesized but the formation of chlorophyll b was suppressed. When plants were transferred from periodic illumination to continuous illumination chlorophyll b was synthesized but the activity of chlorophyllase declined. Chloramphenicol blocked the increase in enzyme activity independent of the light regimes, but cycloheximide inhibited the activity more effectively during growth in the light. The presence of chlorophyllase activity in the leaves is discussed in relation to the chlorophyll-proteins and the membranes known to be present in chloroplasts. It is suggested that the enzyme is synthesized on plastid ribosomes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00390814
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  • 15
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    Interspecific hybridization of phaseolus vulgaris with P. lunatus and P. acutifolius (1978)
    Springer
    Theoretical and applied genetics 52 (1978), S. 209-215 
    Details
    ISSN: 1432-2242
    Keywords: Phaseolus ; Interspecific hybridization ; Embryo culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The influence of genotypic combinations on the growth of hybrid embryos between Phaseolus vulgaris and P. lunatus, and between P. vulgaris and P. acutifolius was examined. All embryos obtained from P. vulgaris × P. lunatus crosses developed only to a stage which appears to be comparable to the pre-heart-shape stage of selfed embryos. Reciprocal crosses were attempted, but pods abscised at a very early stage. Embryos derived from P. vulgaris × P. acutifolius and reciprocal crosses attained the cotyledon stage although no mature seeds were formed. A distinct characteristic of these embryos was the uneven development of the two cotyledons. The rate of growth and final size of these hybrid embryos seemed to be influenced by the genotypes of both parents. Immature embryos were cultured on defined medium and the effects of glutamine and gibberellin (GA3) were examined. Glutamine was effective in increasing the survival rate; gibberellin had no apparent effect. Plants derived from cultured embryos of P. vulgaris × P. lunatus, P. vulgaris × P. acutifolius and P. acutifolius × P. vulgaris were obtained.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00273891
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