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  • Springer Nature  (8.048)
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  • 11
    Digitale Medien
    Digitale Medien
    Some Amino Acid-Folic Acid Interrelationships in Tetrahymena pyriformis (1961)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 8 (1961), S. 0 
    Details
    ISSN: 1550-7408
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Biologie
    Notizen: DL-serine, DL-methionine or DL-serine + DL-methionine in excess inhibited the growth of Tetrahymena pyriformis H. Excess serine was most inhibitory at high concentration of folic acid, whereas the effect of excess methionine or methionine + serine was most pronounced at low levels of folic acid. Inhibition due to excess serine was relieved by raising the level of methionine or by adding pyrimethamine to lower the effective folic acid level, and was intensified by adding Dl.-ethionine or by raising the level of folic acid. Similarly, inhibition due to excess methionine was relieved by supplying more serine or adding DL-ethionine (which reduced the amount of available methionine) and was intensified by adding pyrimethamine. Inhibition by excess methionine + serine was reversed by increasing threonine, provided there was ample guanine present. Low levels of guanine or the presence of 8-azaguanine prevented this reversal. Comparisons are made with the work of others.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1550-7408.1961.tb01214.x
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  • 12
    Digitale Medien
    Digitale Medien
    Autogamy in Frontonia leucas (Ehrbg.) (1961)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 8 (1961), S. 0 
    Details
    ISSN: 1550-7408
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Biologie
    Notizen: Autogamy in Frontonia leucas is described for the first time. The process appears to occur at irregular intervals. From 7 to 10% of the individuals are affected. The beginning of autogamy is marked by a swelling of all the micronuclei which take part in the first two maturation divisions. The third division however affects only one of the second division products. Occasionally two or three may divide. A paroral cone is not prominent. But a small area close to the peristome is distinguishable as the region where the pronuclei fuse. The syn-karyon divides four times. Some of the division products disintegrate, after which 8 to 9 bodies are left which become differentiated into 4 to 5 macronuclear anlagen and 4 micro-nuclei. Mitotic division of the micronuclei results in their increase in number in the daughter individuals after metagamic divisions. Changes in the macronucleus during autogamy consist in its fragmentation and later absorption in the cytoplasm. There is some indirect evidence of a relationship between the dissolution of the old macronucleus and the development of the new.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1550-7408.1961.tb01217.x
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  • 13
    Digitale Medien
    Digitale Medien
    The Role of the Cytoplasm during Reproduction in a Malarial Parasite (Plasmodium lophurae) as Revealed by Electron Microscopy (1961)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 8 (1961), S. 0 
    Details
    ISSN: 1550-7408
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Biologie
    Notizen: An electron microscope study of Plasmodium lophurae maintained in vivo and in vitro provided information concerning the sequence of events during reproduction, and the role of the cytoplasm in this process.Contrary to the generally held opinion that nuclear fissions precede cytoplasmic division, it was found that the last nuclear fission takes place during advanced stages of cytoplasmic segmentation. This study also supplied evidence that in addition to repeated nuclear divisions, a number of changes occur in all major components of the cytoplasm. These changes are considered as preparatory for reproduction. The cytoplasm continues to be active during the formation of merozoites. At this stage a segregation of cytoplasmic components takes place resulting in the incorporation into the offspring of a condensed cytoplasm containing all the organelles. The watery part of the cytoplasm with the lipids and food vacuoles is withheld and at the end of reproduction forms the residual body, a separate structure bound by a membrane.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1550-7408.1961.tb01223.x
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  • 14
    Digitale Medien
    Digitale Medien
    Ophryogleua hypertrophica n.sp. (Giliata Hymenostomatida) (1961)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 8 (1961), S. 0 
    Details
    ISSN: 1550-7408
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Biologie
    Notizen: Ophryoglena hypertrophica is distinguished by its tomites and its pyriform theronts; by its elliptical and flattened macronucleus with 2 or 3 coupled micronuclei; by its large trophont; by its tomont covered with a thin mucous layer at the interior of which are formed 4 or 8 tomites closely bound one to another. Its physiological evolution is characteristic; the tomite when it comes out of the tomont undergoes a secondary encystment and then becomes the theront. Sometimes the tomite is rostrated and is not attracted by the tissues; the tomite undergoes as before a secondary encystment, but divides inside the cyst. This type also produces complete or partial particular palintomies and regularly forms resistant cysts.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1550-7408.1961.tb01225.x
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  • 15
    Digitale Medien
    Digitale Medien
    Chemotaxis and Sexual Behavior in Chlamydomonas (1961)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 8 (1961), S. 0 
    Details
    ISSN: 1550-7408
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Biologie
    Notizen: Intraspecific chemotaxis between gametes was demonstrated in Chlamydomonas moewusii Gerloff var. rotunda nov. var. which was previously used as C. sp. 24 (Tsubo Y. 1957). a heterothallic isogamous species from Japan. The reaction is unidirectional; the “+” gametes are attracted by the “—” gametes or cell-free supernatant of medium in which the :“-” gametes were suspended. In a study with 4 other isogamous heterothallic Chlamydomonas — C. moewusii Gerloff, C. eugametos Moewus, C. reinhardi Dangeard, and C. morewusii Gerloff var. tenuichloris nov. var. — none of them revealed any intraspecific chemotactic behavior. However, as with the “—” gametes of C. moewusii var. rotunda, both mating types of C. moewusii, C. eugametos, and C. moewusii var. tenuichloris were interspecifically attracted by the supernatant of the “—” culture of C. moewusii var. rotunda. Only C. rein- hardi showed no chemotactic behavior in intra- or interspecific combinations.Although chemotaxis occurred in the above-mentioaed combinations, neither agglutination nor pairing ner zygete formation followed at all in the same combinations. The“;–” cells of C. moewusii var. rotunda killed by osmium vaper and then washed no longer produced the chemotactic agent, but did agglutinate with living “+” cells. Therefore, evidently, chemo-taxis is a separate step from agglutination and zygote-for-mation yet does not seem necessary in the mating of isoga-mous Chlamydomonas. Nonetheless, since this activity appears not in the vegetative but in the gametic stage, it seems to concern the sexual activity of the cells. In preliminary studies the chemotactic agent produced by C. moewusii var. rotunda was shown to be volatile.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1550-7408.1961.tb01191.x
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  • 16
    Digitale Medien
    Digitale Medien
    Infection of Hydra by Microsporidia (1961)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 8 (1961), S. 0 
    Details
    ISSN: 1550-7408
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Biologie
    Notizen: A microsporidian infection in a laboratory clone of Hydra littoralis has been observed, and the parasite has been tentatively identified as a species of Plistophora. Infected hydra continue to bud and regenerate normally and show no significant physiological or morphological changes. Sexual crossing of infected and non-infected animals shows that the infection is transmitted by the ovum but not by the sperm. Continuous exposure of infected hydra to Fumidil B in solution resulted in the disappearance of all Plistophora spores after a five week period of treatment, and the clones of the treated animals have remained parasite-free for more than a year.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1550-7408.1961.tb01197.x
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  • 17
    Digitale Medien
    Digitale Medien
    Amino Acid Composition of Protozoa. Comparative Studies on Tetrahymena (1961)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 8 (1961), S. 0 
    Details
    ISSN: 1550-7408
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Biologie
    Notizen: Patterns for free and protein amino acids and related substances were determined for Tetrahymena limacis and 7 strains of T. pyriformis from axenic stationary phase cultures grown at 25° C by means of 2-dimensional chromatography in a butanol-acetic acid and phenol solvent system with ninhydrin and other polychromatic indicators. A uniform protein amino acid (PAA) pattern was observed in all strains. There were 14 color spots indicating 19 amino acids (including cysteic acid), identified as follows: alanine, arginine, aspartic acid, cysteine/cystine, cysteic acid, glutamic acid, glycine, leucine/isoleucine/phenylalanine, lysine/histidine, proline, serine, threonine, tyrosine, valine/methionine.The following free amino acids and related substances (FAAs) were identified with 14 spots (several different from these for PAA patterns) found in all strains: alanine, arginine, asparagine, aspartic acid, glutamic acid, glutamine, glycine, leucine/isoleucine/phenylalanine, lysine histidine. proline, serine, threonine, tyrosine, valine/methionine. T. limacis and strains LI and Gf-J of T. pyriformis exhibited these only. Chromatograms of 5 strains of T. pyriformis (PR. F. L3 WH52, HS), however, also contained 1 to 4 spots representing certain of the following substances: Cysteic acid, cysteine/cystine, taurine, and the unknowns X1, X2, and X3, having Rf's of 0.33, 0.79, and 0.72 respectively in 4:1 phenol-H2O system Excepting for F and L3, which were similar, the T. pyriformis strains showed quite different distributional patterns of these substances at 25°C. Other deviations in the distribution of the 6 compounds were noted in the chromatograms of 10° and 35° cultures of WH52 and HS. These findings on FAAs and PAAs are tabulated, along with those of previous investigators, to furnish comparisons on 13 strains of Tetrahymena pyriformis, T. limacis and 9 other species of protozoa.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1550-7408.1961.tb01202.x
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  • 18
    Digitale Medien
    Digitale Medien
    The Biology and Morphology of Crithidia acanthocephali n. sp., Leptomonas leptoglossi n. sp., and Blastocrithidia euschisti n. sp. (1961)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 8 (1961), S. 0 
    Details
    ISSN: 1550-7408
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Biologie
    Notizen: Three new species of Trypanosomatidae were isolated from three species of bugs: Leptomonas leptoglossi from Leptoglossus phyllopus, Crithidia acanthocephali from Acanthocephala femorata, and Blastocrithidia euschisti from Euschistus servus. All were cultured axenically and on avian embryo membranes. In addition to differences in morphology the three organisms displayed different growth rates in the chorio-allantoic fluids of duck and chick embryos incubated at 30°C. L. leptoglossi grew most abundantly. B. euschisti barely maintained itself while C. acanthocephali occupied an intermediate position.When the temperature of incubation was raised to 37°C, there was continued multiplication of L. leptoglossi and C. acanthocephali, but there was no growth of B. euschisti in either duck or chick embryos.It is suggested that the criteria of morphology, cultural characteristics in vitro and in vivo, plus physiological characters be used as future aids in classification of the Trypanosomatidae.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1550-7408.1961.tb01204.x
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  • 19
    Digitale Medien
    Digitale Medien
    Anaerobic Metabolism of Crithidia fasciculata (1961)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 8 (1961), S. 0 
    Details
    ISSN: 1550-7408
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Biologie
    Notizen: SYNOPSIS: Crithidia fasciculata was cultured in a modification of the nutrient medium described by Cowperthwaite in 1951. Carbon dioxide, lactic acid, succinic acid and ethyl alcohol were produced by the organisms during anaerobic conditions. Hexokinase, enolase, alcohol dehydrogenase and glucoses-phosphate dehydrogenase were demonstrated in ho-mogenates of the flagellates. Aldolase, phosphohexokinase and lactic acid dehydrogenase could not be demonstrated.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1550-7408.1961.tb01173.x
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  • 20
    Digitale Medien
    Digitale Medien
    Fine Structure of the Silverline and Fibrillar Systems of Three Tetrahymenid Ciliates (1961)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 8 (1961), S. 0 
    Details
    ISSN: 1550-7408
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Biologie
    Notizen: Application of fragmentation and thin-sectioning techniques to Tetrakymena pyriformis, Colpidium compiles and Glaucoma chattoni has permitted an analysis of the ultra-structure of their silverline and fibrillar systems. The classical silverline system consists of a mosaic of flat, membrane-bound blisters whose rims represent the sites of selective silver deposition. Cilia and protrichocysts emerge between adjacent blisters. I The pellicle consists of the membranes outlining the blisters, overlain by a continuous outer membrane that covers the whole cell and cilia. Fibrillar structures, which are not argentophilic, include: (1) tapering, striated kinetodesmal fibers arising singly from the kinetosomes, passing to the right and anteriad, and overlapping to form a loose bundle accompanying each kinety; (2) a longitudinal fibril band immediately beneath the pellicle at the right of each kinety, consisting of overlapping individual fibrils; (3) a transverse band of fibrils arising at the left side of each kinetosome and passing to the left under the pellicle; and (4) a set of postciliary fibrils arising at the right posterior edge of each kinetosome and passing posteriad under the pellicle. The fibrils of sets (2), (3), and (4) all are about 20 Mμ in diameter and appear tubular in cross-section; they are very unlike the heavier, solid kinetodesmal fibers. None of the fibril sets directly interconnect, although transverse and postciliary fibrils end in the vicinity of the longitudinal fibril band.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1550-7408.1961.tb01186.x
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