ALBERT

All Library Books, journals and Electronic Records Telegrafenberg

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
Filter
  • Articles  (12)
  • Autoradiography  (12)
  • Springer  (12)
  • American Meteorological Society
  • American Physical Society (APS)
  • Blackwell Publishing Ltd
  • Cambridge University Press
  • De Gruyter
  • Springer Nature
  • Wiley
  • 2000-2004
  • 1980-1984  (12)
  • 1960-1964
  • 1935-1939
  • 1930-1934
  • 2001
  • 1984  (12)
  • 1956
  • 1938
  • 1936
  • Medicine  (12)
  • Geosciences
  • Process Engineering, Biotechnology, Nutrition Technology
Collection
  • Articles  (12)
Source
Keywords
Publisher
Years
  • 2000-2004
  • 1980-1984  (12)
  • 1960-1964
  • 1935-1939
  • 1930-1934
Year
Topic
  • Medicine  (12)
  • Geosciences
  • Process Engineering, Biotechnology, Nutrition Technology
  • Biology  (13)
  • 1
    Electronic Resource
    Electronic Resource
    Autoradiographic localization of ornithine decarboxylase in mouse kidney by use of radiolabeled α-difluoromethylornithine (1984)
    Springer
    Cell & tissue research 235 (1984), S. 371-377 
    Details
    ISSN: 1432-0878
    Keywords: Ornithine decarboxylase ; Polyamines ; Mouse ; kidney ; Autoradiography
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Ornithine decarboxylase, a key enzyme in polyamine biosynthesis and cell growth, has been localized in mouse kidney by autoradiography after administration of radiolabeled α-difluoromethylornithine. This drug is an enzyme-activated irreversible inhibitor of ornithine decarboxylase and forms a covalent bond with the enzyme. It was found that ornithine decarboxylase is present in all cell types studied but that the highest content occurs in the proximal convoluted tubules followed by the distal convoluted tubules and the collecting tubules. The majority of the enzyme is located in the cytoplasm but about 10–15% is present in the nuclei (often associated with nucleolus-like components) of the cells of the proximal and distal convoluted tubules. The labeled ornithine decarboxylase was lost rapidly from both nucleus and cytoplasm of all the cell types examined, and labeling by radioactive α-difluoromethylornithine was greatly reduced if the mice were pretreated for 5 h with cycloheximide to block protein synthesis. These results indicate that ornithine decarboxylase turns over rapidly in all of the cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00217862
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Pathways of lymphocyte migration within the periarterial lymphoid sheath of rat spleen (1984)
    Springer
    Cell & tissue research 236 (1984), S. 661-667 
    Details
    ISSN: 1432-0878
    Keywords: Lymphocytes ; Spleen ; Autoradiography
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Male Wistar rats were injected intravenously with 5-(3H)uridine-labeled lymphocytes isolated from lymph nodes of syngeneic donors and enriched in T cells. After short periods of time (3 to 120 min after injection), labeled lymphocytes were localized in spleen compartments using autoradiography to identify routes of lymphocyte movement from blood into splenic parenchyma and to follow migration pathways of recirculating lymphocytes within the periarterial lymphoid sheath (PALS). Topographical analysis of labeled lymphocytes was performed in specific planes of PALS characterized by the diameter of the arterial vessel and termed PALS large, PALS medium, and PALS small (PALS L, PALS M, PALS S, respectively). Attention was also paid to accumulations of labeled lymphocytes close to the arterial vessel wall. Initially, labeled lymphocytes were localized in PALS S and PALS M near the terminal branching of arterial vessels and in the marginal zone (MZ). We conclude that lymphocytes emigrate from blood into splenic parenchyma within two white pulp compartments: in MZ, and directly within PALS through the wall of capillary vessels. The sequential accumulation of labeled cells near arterial vessels of increasing diameter suggests that the recirculating pool of lymphocytes migrates into the central part of PALS L by two routes: from MZ, and along arterial vessels from PALS S and PALS M.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00217236
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Homing of germinal-center cells into germinal centers of lymph node via afferent lymphatics (1984)
    Springer
    Cell & tissue research 238 (1984), S. 183-189 
    Details
    ISSN: 1432-0878
    Keywords: Lymphocyte migration ; Germinal centers ; Autoradiography ; Lymph nodes ; Lymphatic vessels ; B-lymphocytes, differentiation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Affinity of lymphoid cells for the microenvironment of germinal centers (GC), as detectable in transfer experiments by rapid homing in spleen GC from the blood, is a capacity expressed by only a subset of lymphoid cells, in particular by those constituting a GC. However, when introduced into the blood stream, these cells do not home into GC of lymph nodes and gut-associated lymphoid tissues. To investigate further this homing inability for high endothelial venule (HEV)-containing lymphoid tissues, GC cells isolated from donor rabbit appendix were labeled in vitro with 3H-leucine and injected into an afferent lymph vessel of recipient popliteal lymph nodes. Draining lymph nodes were removed 15 min to 24 h after cell administration and prepared for radioautography. For reference, the migration of cells isolated from Peyer's patches and thoracic duct lymph was also studied. By use of appendix GC cells, large numbers of labeled cells were found to migrate into GCs of the outer cortex centripetally, i.e., from the subcapsular sinus through the lymphocyte corona into the GC proper. The same was observed for cells from Peyer's patches, although in smaller numbers. Thoracic duct lymphocytes were only localized in the lymphocyte corona and the deep cortex. Thus, appendix GC cells and a subpopulation of cells from Peyer's patches can reach lymph node GC, but only when administered intralymphatically. We conclude that cells expressing affinity for the GC microenvironment do so for both spleen and lymph node GC, but do not have the capacity to interact with the wall of HEV; its implication for the understanding of the dynamics of a GC reaction is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00215160
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    Topographical and developmental studies on target sites of 1,25 (OH2) vitamin D3 in skin (1984)
    Springer
    Cell & tissue research 238 (1984), S. 489-496 
    Details
    ISSN: 1432-0878
    Keywords: Vitamin D ; Skin ; Autoradiography ; Hair ; Development
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Tritium-labeled 1,25 (OH2) vitamin D3, when injected into vitamin D-deficient adult and pregnant rats is concentrated and retained strongest in nuclei of cells in the outer root sheath of the hair, followed by the stratum granulosum, spinosum, and basale of the epidermis. In the hair follicle, in addition to the most heavily labeled outer root sheath, nuclear labeling exists also in cells of the hair bulb and of the inner root sheath, as well as in basal cells of the sebaceous gland. In contrast, cells of the dermal papilla and the connective tissue of the dermis are generally unlabeled, except for labeled cells in the outer connective tissue sheath at the infundibulum of vibrissae of 20-day fetal rats and a few scattered labeled cells in the dermis, probably macrophages. In the developing hair, in 18- and 20-day fetal rats, a distinct topographic pattern of labeled cells can be seen, which is characteristic of the different stages of hair follicle development. In the hair germ, heavily labeled cells appear first in the stratum spinosum. In the hair peg, they remain in this position in its juxtaepidermal portion; however, when a dermal papilla develops, heavily labeled cells assume a marginal position. This suggests a sequential epidermal-epidermal and mesenchymal-epidermal receptor induction. Injection of tritium labeled 25 (OH) vitamin D3 did not show nuclear concentration in these tissues and excess unlabeled 25 (OH) vitamin D3 — unlike excess 1,25 (OH2) vitamin D3 — did not prevent nuclear uptake of tritium labeled 1,25 (OH2) vitamin D3. The results indicate differential effects of 1,25 (OH2) vitamin D3 on different structures in the epidermis and dermis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00219863
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 5
    Electronic Resource
    Electronic Resource
    Localization of GABA high-affinity binding sites in the pancreas of neonatal rat (1984)
    Springer
    Cell & tissue research 235 (1984), S. 503-508 
    Details
    ISSN: 1432-0878
    Keywords: γ-Aminobutyric acid (GABA) ; Autoradiography ; Pancreas, neonatal rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The localization of gamma-aminobutyric acid (GABA) high-affinity binding sites was investigated in the exocrine and endocrine pancreas of neonatal rats by means of 3H-GABA autoradiography. GABA-binding was identified on Schwann cells and on the cells of the intralobular excretory ducts. In the endocrine part of the pancreas, no labelling was observed except in peripheral islet cells which, on the basis of their scarcity and distribution, could be somatostatin cells. Furthermore, peri-insular innervation showed considerable labelling.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00226946
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 6
    Electronic Resource
    Electronic Resource
    Autoradiographic demonstration of α2 adrenoceptors in the bovine neurohypophysis (1984)
    Springer
    Cell & tissue research 237 (1984), S. 387-389 
    Details
    ISSN: 1432-0878
    Keywords: Pituitary gland, pars nervosa ; Autoradiography ; α2Adrenoreceptors ; Clonidine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Autoradiography of sections from neurohypophyses treated with tritiated clonidine has shown specific binding to α2-adrenoceptor sites in the neurohypophysis but not in the intermediate lobe. Other studies have shown that α2 agonists such as clonidine can cause a fall in circulating antidiuretic hormone; it is therefore possible to speculate that this action could be a direct one on the neurohypophysis since the appropriate binding sites have been shown to exist.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00217164
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 7
    Electronic Resource
    Electronic Resource
    Development and ultrastructural autoradiographic studies of nucleolus-like bodies (nuages) in oocytes of a viviparous teleost (Xiphophorus helleri) (1984)
    Springer
    Cell & tissue research 238 (1984), S. 121-128 
    Details
    ISSN: 1432-0878
    Keywords: Ultrastructure ; Autoradiography ; Oocytes ; Nucleolus-like bodies ; Teleost
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Cytoplasmic granulo-fibrillar masses, usually termed nucleolus-like bodies (NLB) or nuages, have been described in several different cell types. They are sometimes associated with a mitochondrial arrangement, this association often being marked during certain phases of the oocyte cycle. In Xiphophorus helleri, NLB consist of fibrillar and granular material that gradually becomes more granular during meiotic prophase I, and is associated with mitochondrial arrangements during diplotene and dictyate of meiosis. Autoradiographic studies of uridine incorporation into the nucleolus and subsequently into NLB suggest that the latter represent a reserve of ribonucleoproteins that is later used in ribosomal maturation during vitellogenesis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00215152
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 8
    Electronic Resource
    Electronic Resource
    Synthesis and transport of the organic matrix of the spicules in the gorgonian Leptogorgia virgulata (Lamarck) (Coelenterata: Gorgonacea) (1984)
    Springer
    Cell & tissue research 235 (1984), S. 533-538 
    Details
    ISSN: 1432-0878
    Keywords: Gorgonians ; Organic matrix ; Autoradiography ; Calcification ; Leptogorgia virgulata
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The sequence of the synthesis and transport of the organic matrix of spicules has been elucidated in the gorgonian Leptogorgia virgulata by use of 3H-aspartic acid as the tracer in electron-microscopic autoradiography. The entire process of matrix synthesis and transport takes approximately 2 h. It seems that the protein moiety of the organic matrix is synthesized in the RER prior to 5 min following the initial 10 min incubation in the tracer. At the 5 min chase the label is moving from the RER to the Golgi complexes where the carbohydrate moiety of the matrix is presumed to be synthesized. At the 5 to 15 min chases the label is transported out of the Golgi complexes via Golgi vesicles. This phase continues for 30 min. From 60 to 120 min the 3H-aspartic acid moves to the spicules. After 120 min the majority of the label has moved into the spicules. Silver grain counts over both multivesicular and electron-dense bodies remain at relatively low and constant levels over 4 h indicating that neither organelle is involved in the synthesis and transport of the organic matrix.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00226950
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 9
    Electronic Resource
    Electronic Resource
    In-vitro uptake and metabolism of [3H]-5-hydroxytryptamine in the pineal glands of the rabbit, rat and hamster (1984)
    Springer
    Cell & tissue research 235 (1984), S. 539-549 
    Details
    ISSN: 1432-0878
    Keywords: Pineal gland ; Mammals ; Autoradiography ; [3H]-indoles
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Pineal glands of rat, rabbit and hamster were incubated during day or night in Merlis' fluid containing [3H]-5-hydroxytryptamine (= [3H]-HT) by the use of a 20-min pulse with or without postincubation in “cold” medium for 15, 30, 45 or 60 min. (1) Selective autoradiographic labeling was observed in sympathetic nerve terminals; this reaction was missing after bilateral surgical removal of the superior cervical ganglia. In contrast, a scarce and diffuse labeling was found in pinealocytes (Pi) and interstitial cells (IC) of both untreated and ganglionectomized animals. (2) With the use of thin-layer chromatography, it could be shown in the rat that the well-known indoles of the pineal gland are formed from [3H]-HT. (3) During preparation for electron microscopy (EM), the total loss of indoles from pineal glands was studied by means of liquid-scintillation counting; approximately 57% of the radioactivity of the pineal glands was released into EM-processing solutions, mainly into the glutaraldehyde fixative. In summary, our results show that in this type of experiment with pineal glands of mammals, the routinely used EM-procedures are inadequate to visualize the uptake and metabolism of exogenous indoles in Pi and IC. Furthermore, the data differ considerably from those obtained with the pineal organs of several reptilian and avian species when a similar cytological procedure is used. It appears that protein(s) located in the densely packed vesicles of the pineal cells of sauropsids, homologous to mammalian pinealocytes, may play a crucial role in indole binding (specific indole-binding proteins); this may help to interpret the diverging results obtained in different amniotes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00226951
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 10
    Electronic Resource
    Electronic Resource
    On the uptake and storage of 5-hydroxytryptamine, 5-hydroxytryptophan and catecholamines by adrenal chromaffin cells and nerve endings (1984)
    Springer
    Cell & tissue research 236 (1984), S. 189-195 
    Details
    ISSN: 1432-0878
    Keywords: Adrenal medulla ; 5-Hydroxytryptamine ; 5-Hydroxytryptophan ; Noradrenaline ; Autoradiography
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Light-microscopic autoradiographs of the adrenal medulla at various intervals after the intravenous injection of [3H] 5-HTP, [3H] 5-HT, [3H] noradrenaline and [3H] adrenaline have been studied. The distribution of silver grains following [3H] 5-HTP uptake was found to be uniform over each of the two main cell populations, adrenaline-storing (A) cells and noradrenaline-storing (NA) cells in the adrenal medulla, but A cells were twice as active as NA cells in incorporating the isotope, a situation very similar to that found after [3H] dopa uptake. 5-HT administration resulted in a pattern resembling the distribution of [3H] noradrenaline uptake, with A cells being 4 or 5 times more active than NA cells and a gradient of activity from the periphery of the medulla inwards. However, the time-course for the loss of radioactivity was not the same for both amines: levels of 5-HT activity were not significantly reduced after one week whereas the degree of [3H] noradrenaline labelling after one week was less than 10% of that at one hour. Thus 5-HT may be bound to sites in the adrenal medulla normally occupied by noradrenaline but it would appear that the release mechanism is different. There was no evidence of 5-HT uptake by adrenal nerve endings.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00216530
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...